Protein cysteine endopeptidases. Medical search. Frequent questions

This approach uses degenerate oligonucleotides encoding the highly conserved pentapeptides QACRG and GSWFI that are present in all known apoptotic cysteine proteases. (deathbase.org)
Subfamily-Specific Fluorescent Probes for Cysteine Proteases Display Dynamic Protease Activities during Seed Germination. (ox.ac.uk)
Cysteine proteases are an important class of enzymes implicated in both developmental and defense-related programmed cell death and other biological processes in plants. (ox.ac.uk)
Because there are dozens of cysteine proteases that are posttranslationally regulated by processing, environmental conditions, and inhibitors, new methodologies are required to study these pivotal enzymes individually. (ox.ac.uk)
Here, we introduce fluorescence activity-based probes that specifically target three distinct cysteine protease subfamilies: aleurain-like proteases, cathepsin B-like proteases, and vacuolar processing enzymes. (ox.ac.uk)
When applied on germinating seeds, these probes reveal dynamic activities of aleurain-like proteases, cathepsin B-like proteases, and vacuolar processing enzymes, coinciding with the remobilization of seed storage proteins. (ox.ac.uk)
This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. (enigmadiagnostics.com)
Furthermore, Bromelain Plus CLA contains ficin derived from figs. Ficin is a highly active member of the cysteine endopeptidase family of proteases that not only hydrolyzes protein like materials, but it also has vermifuge properties. (letstruck.com)

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. (cancerindex.org)
USP40 has several biochemical functions, for example, cysteine-type endopeptidase activity, ubiquitin-specific protease activity. (creativebiomart.net)
Cathepsin B, also known as CTSB, is a lysosomal cysteine protease. (biolegend.com)
Papain is a cysteine protease enzyme (EC 3.4.22.2) that has the endopeptidase activity to cleave immunoglobulin G molecules in the hinge reason. (gbiosciences.com)
Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. (deathbase.org)
We have developed a PCR approach to clone new apoptotic Ced-3/Ice-like cysteine protease genes. (deathbase.org)
Our data suggest that Mch2 is a Ced-3/interleukin-1 beta converting enzyme-like cysteine protease and could be another important mediator of apoptosis in mammalian cells. (deathbase.org)
Pripper is not restricted to predicting only caspase cut sites, but it gives the possibility to scan protein sequences for any given motif(s) and predict cut sites once a suitable cut site prediction model for any other protease has been developed. (biomedcentral.com)

Disengagement of mom and daugther centriole is usually a prerequisite for centriole duplication and it is offered by proteolytic cleavage of cohesin, a,glue, protein complex that is certainly also responsible for sister chromatide cohesion. (pkc-inhibitors.com)
OTUB1, YpkA and the small GTPase ras homologue gene family member A (RhoA) were found to be part of the same protein complex, suggesting that RhoA levels are modulated by OTUB1. (ox.ac.uk)
Striations result from the complex organization of the myofibrillar proteins, responsible for the muscle contraction. (earthwormexpress.com)

Multiple gene mutations have been identified and considered as important substrates for the development of MDS, such as RNA splicing, histone manipulation, DNA methylation, transcription factors, kinase signaling, DNA repair, cohesin proteins, and other signal transduction elements. (frontiersin.org)

For immunization with such antigens, proteins should be less suitable than peptides, which do not require proteasome digestion in dendritic cells. (ox.ac.uk)

Cysteine peptidases with a tertiary structure similar to that of the serine-type aspartyl dipeptidase are included in clan PC. (embl.de)

FUNCTIONS IN: cysteine-type endopeptidase activity, cys. (riken.jp)
This was most likely the result of an increase in the gene expression and enzyme activity of L-cysteine desulfhydrase, which is responsible for H2S synthesis. (bvsalud.org)
Functional assays confirm the identity of a catalytic cysteine in these endopeptidases and crystal structures provide information on the structure-activity relationships of Ssp1 and, by comparison, of related effectors. (dundee.ac.uk)
We report on separase transcription, protein expression, and Separase proteolytic activity. (pkc-inhibitors.com)
Predicted to allow carboxypeptidase activity and cysteine-type endopeptidase activity. (enigmadiagnostics.com)
It has similarity to the SABP2 methyl salicylate esterase from tobacco but no enzymatic activity has been identified for this protein. (or.jp)
They appear to be involved in some of the same fundamental cell activities as regular proteasomes, such as regulating the amount of various proteins in cells (protein homeostasis), cell growth and division, the process by which cells mature to carry out specific functions (differentiation), chemical signaling within cells, and the activity of genes. (medlineplus.gov)
Sjöberg A., Trouw L., McGrath F., Hack E. C. and Blom A. M. (2006) Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of C4b-binding protein. (lu.se)

Chloroplast-located proteins which are encoded by the nuclear genome have to be imported from the cytosol into the organelle in a post-translational manner. (cipsm.de)

The protein encoded by this gene is a lysosomal cysteine proteinase and a member of the C1 peptidase family. (enigmadiagnostics.com)

This enzyme catalyses the following chemical reaction: Hydrolysis of proteins with broad specificity for peptide bonds, similar to those of papain and chymopapain This enzyme is isolated from the papaya plant, Carica papaya. (wikipedia.org)
The protein is 216 amino acids in length, and is 68% identical in sequence to papain, 65% to chymopapain and 81% to glycyl endopeptidase. (wikipedia.org)
Clan CA includes proteins with a papain-like fold. (embl.de)
Following papain digestion the Fab fragments are separated from undigested IgG and the Fc region with the supplied Protein A Spin Column. (gbiosciences.com)
In addition to these enzymes, Bromelain Plus CLA also contains inositol, papain, ficin, and the amino acid L-cysteine. (letstruck.com)

By the time the nuclear DNA was susceptible to heavy label by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling, the ricinosomes had released into the cytoplasm their content of cysteine endoprotease, which became activated because of the cleavage of its propeptide. (bibbase.org)
Koh H, Lee KH, Kim D, Kim S, Kim JW, Chung J: Inhibition of Akt and its anti-apoptotic activities by tumor necrosis factor-induced protein kinase C-related kinase 2 (PRK2) cleavage. (drugbank.com)
Separase, a cysteine endopeptidase, conducts cleavage of cohesin. (pkc-inhibitors.com)
Caspases mediate their effects through aspartate-specific cleavage of their target proteins, and at present almost 400 caspase substrates are known. (biomedcentral.com)
There are several methods developed to predict caspase cleavage sites from individual proteins, but currently none of them can be used to predict caspase cleavage sites from multiple proteins or entire proteomes, or to use several classifiers in combination. (biomedcentral.com)
Three different pattern recognition classifiers were developed for predicting caspase cleavage sites from protein sequences. (biomedcentral.com)
We have developed Pripper, a tool for reading an arbitrary number of proteins in FASTA format, predicting their caspase cleavage sites and outputting the cleaved sequences to a new FASTA format sequence file. (biomedcentral.com)
Caspases mediate their effects through aspartate-specific cleavage of their target proteins, and several hundreds of caspase target proteins have been identified [ 3 ]. (biomedcentral.com)
The prediction of caspase cleavage sites from proteins is not a trivial task due to the heterogeneity of target sequences, and several different tools have been developed to predict caspase cleavage sites from individual proteins. (biomedcentral.com)

While most cathepsins are exclusively endopeptidases, CTSB exhibits both carboxypeptidase and endopeptidase activities. (biolegend.com)

Wang HG, Rapp UR, Reed JC: Bcl-2 targets the protein kinase Raf-1 to mitochondria. (drugbank.com)

Are there multiple proteolytic pathways contributing to c-Fos, c-Jun and p53 protein degradation in vivo? (cnrs.fr)
What pathways are this gene/protein implicaed in? (cancerindex.org)
In addition, proteins with the corresponding master regulatory pathways had been analyzed. (pkc-inhibitors.com)

After the pathogenic bacterium Legionella pneumophila is phagocytosed, it injects more than 250 different proteins into the cytoplasm of host cells to evade lysosomal digestion and to replicate inside the host cell. (cipsm.de)

A cysteine endopeptidase from adenoviruses, the type example of peptidase family C5 , with a protein fold unlike that known for any other peptidase [2]. (enzyme-database.org)

This entry represents the catalytic domain of UCH proteins of the UBP1 group. (embl.de)

Cysteine peptidases are often active at acidic pH and are therefore confined to acidic environments, such as the animal lysosome or plant vacuole. (embl.de)

Activation of this enzyme can occur via the interaction of its caspase recruitment domain with CARD SIGNALING ADAPTOR PROTEINS. (umassmed.edu)
Here we report that beta-amyloid precursor protein (APP) and death receptor 6 (DR6, also known as TNFRSF21) activate a widespread caspase-dependent self-destruction program. (deathbase.org)
A database was constructed with the developed tool, and it was used to identify caspase target proteins from tandem mass spectrometry data from two different proteomic experiments. (biomedcentral.com)
We show that Pripper is a valuable tool in identifying novel caspase target proteins from modern proteomics experiments. (biomedcentral.com)

The cells of the endosperm of castor bean seeds (Ricinus communis) undergo programmed cell death during germination, after their oil and protein reserves have been mobilized. (bibbase.org)

All taken together, these observations underline the complexity of the mechanisms responsible for the selective destruction of proteins within cells. (cnrs.fr)
The nutrients in Elite Endurance Protein enhance overnight muscle reconditioning through muscle repair, recovery & and development, help relieve stress, relax muscles and rejuvenate cells and tissues, induce calmness, and promote a strong gut and sleep. (unived.in)
Infestation with WCM (apart from or with CCN) also significantly restricted the electron transport on the photosystem I acceptor side, therefore produced reactive oxygen species oxidized lipids in cells of WCM and double infested plants and proteins in cells of WCM-infested plants. (bvsalud.org)
In all living cells, protein synthesis occurs on ribonucleoprotein particles called ribosomes. (cipsm.de)
The CTL failed to lyse autologous EBV-transformed B cells, even though the latter express the protein. (ox.ac.uk)
Immunoproteasomes are specialized versions of proteasomes, which are large complexes that recognize and break down (degrade) unneeded, excess, or abnormal proteins within cells. (medlineplus.gov)
Because the protein produced from the PSMB8 gene may be involved in the maturation of adipocytes, studies suggest that a shortage of this protein may interfere with the normal development and function of these cells. (medlineplus.gov)
Trouw L., Nilsson S., Goncalvez I., Landberg G. and Blom A. M. (2005) C4b-binding protein binds to necrotic cells and DNA, which limits DNA release and inhibits complement activation. (lu.se)
Epithelial cells adjacent to the area of the defect flatten, lose their hemidesmosome attachments, and migrate on transient focal contact zones that are formed between cytoplasmic actin filaments and extracellular matrix proteins. (medscape.com)

Elite Endurance Protein is specifically formulated with 30 grams per serving of a complete blend of both essential and non-essential amino acids sourced from pea isolate, which is a complete protein. (unived.in)
Studies of amino acids in the motif have revealed that the motif is only moderately conserved between target proteins of different caspases. (biomedcentral.com)

The cysteine endoprotease is distinguished by a C-terminal KDEL sequence, although it is not retained in the lumen of the endoplasmic reticulum and is a marker for ricinosomes. (bibbase.org)
Comparisons with Ssp2-Rap2a orthologues suggest that the specificity of these immunity proteins for neutralizing effectors is fold-dependent and that in cases where the fold is conserved sequence differences contribute to the specificity of effector-immunity protein interactions. (dundee.ac.uk)

The new gene encodes a approximately 34-kilodalton protein that is highly homologous to human CPP32, Caenorhabditis elegans cell death protein CED-3, mammalian Ich-1 (Nedd2), and mammalian interleukin-1 beta converting enzyme. (deathbase.org)
Encodes a protein predicted to act as a carboxylesterase. (or.jp)

A cysteine peptidase is a proteolytic enzyme that hydrolyses a peptide bond using the thiol group of a cysteine residue as a nucleophile. (embl.de)
All of these alterations have deep effects on muscle proteins and also on proteolytic enzyme systems (Huff Lonergan et al. (earthwormexpress.com)

Caricain is regarded as a cysteine endopeptidase, that is, it functions through the action of a cysteine residue at its active site and it is capable of hydrolysing peptide bonds that are well within the N-terminus and C-terminus of the substrate. (wikipedia.org)
Hydrolysis involves usually a catalytic triad consisting of the thiol group of the cysteine, the imidazolium ring of a histidine, and a third residue, usually asparagine or aspartic acid, to orientate and activate the imidazolium ring. (embl.de)
In only one family of cysteine peptidases, is the role of the general base assigned to a residue other than a histidine: in peptidases from family C89 (acid ceramidase) an arginine is the general base. (embl.de)

Trypsin is a serine endopeptidase that specifically cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues. (gbiosciences.com)

MMPs are an important family of metal-dependent endopeptidases and represent the major class of enzymes responsible for the degradation or resorption of all components of the extra-cellular matrix (ECM) 38 . (bvsalud.org)

During growth, genes associated with protein ubiquitination/deubiquitination, cell cycle progression, and proteasomal degradation were affected, while genes linked to protein and carbohydrate catabolism were affected during early development. (frontiersin.org)
Overall, this study reveals the impact of cln5 -deficiency on gene expression in D. discoideum , provides insight on the genes and proteins that play a role in regulating Cln5-dependent processes, and sheds light on the molecular mechanisms underlying CLN5 disease. (frontiersin.org)

Four clans of cysteine peptidases share structural similarities with serine and threonine peptidases and asparagine lyases. (embl.de)
Blom A. M. , Kask L. and B. Dahlbäck (2001) Structural requirements for the complement regulatory activities of C4b-binding protein. (lu.se)
Kask L., Hillarp A., Ramesh B., Dahlbäck B., and Blom A. M. (2002) Structural requirements for the intra-cellular subunit polymerization of the complement inhibitor C4b-binding protein. (lu.se)

However, like most CLN proteins, the association of CLN5 with a defined biological pathway is still under investigation. (frontiersin.org)

These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins. (embl.de)

The identified mutation changes a single protein building block (amino acid) in the protein produced from the PSMB8 gene, replacing the amino acid glycine with the amino acid valine at protein position 201 (written as Gly201Val or G201V). (medlineplus.gov)
This mutation greatly reduces the production of this protein, which impairs the normal assembly of immunoproteasomes and causes the immune system to malfunction. (medlineplus.gov)
This mutation replaces the amino acid threonine with the amino acid methionine at protein position 75 (written as Thr75Met or T75M). (medlineplus.gov)

Members of the Alb3/Oxa1/YidC protein family function as insertases in chloroplasts, mitochondria, and bacteria. (cipsm.de)
The encoded protein is also known as cathepsin X and cathepsin P. This gene is ubiquitously expressed in cancer cell lines and primary tumors and, like other members of this family, may be involved in tumorigenesis. (enigmadiagnostics.com)
In this study, susceptibility to Yersinia enterocolitica and Yersinia pseudotuberculosis invasion was found to be increased upon overexpression of the deubiquitinating enzyme otubain 1 (OTUB1), a member of the ovarian tumour domain-containing protein family. (ox.ac.uk)

An enzymatically inactive form of the protein, that is associated with the propeptide, may be involved in cancer cell invasion and proliferation. (enigmadiagnostics.com)

Contiene varios DEDOS DE ZINC y actúa en la respuesta inmunitaria y en la INFLAMACIÓN modulando las señales del TNF-ALFA, la IL1-BETA o de patógenos vía RECEPTORES TIPO TOLL parando la actividad de NF-KAPPA B. (bvsalud.org)

As with some other plant cysteine endopeptidases, caricain exhibits charge heterogeneity. (wikipedia.org)

Clean Organic Plant-Based Vegan Protein! (unived.in)

USP40 has direct interactions with proteins and molecules. (creativebiomart.net)
We selected proteins and molecules interacted with USP40 here. (creativebiomart.net)
The Protein A Resin binds the IgG and Fc molecules and the Fab are rapidly collected due to the spin-format design. (gbiosciences.com)

Researchers speculate that mutations in different areas of the gene may have different effects on protein function. (medlineplus.gov)

Chemicals and Drugs24

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes6

Information Science3

Proteases8

Protease8

Complex3

Signal Transduction1

Peptides1

Serine-type1

Activity8

Post-translational1

Lysosomal cysteine1

Papain5

Cleavage9

CTSB1

Kinase1

Pathways3

Cytoplasm1

Peptidase family1

Catalytic domain1

Vacuole1

Caspase4

Germination1

Cells9

Amino acids2

Sequence2

Encodes2

Proteolytic enzyme2

Residue3

Peptide bonds1

Class of enzymes1

Genes2

Structural3

Biological1

Enzymes1

Mutation3

Family3

Propeptide1

Zinc1

Exhibits1

Plant1

Molecules3

Function1