Protein culture media conditioned. Medical search. Frequent questions

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. (sigmaaldrich.com)

Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
Here we generated a novel, two-sided fusion protein incorporating the extracellular domains of SIRPα and CD40L, adjoined by a central Fc domain, termed SIRPα-Fc-CD40L. (aacrjournals.org)
Common culture formats used for in vitro enzyme induction studies comprise conventional and sandwich cultures, the former referring to plated hepatocytes maintained on rigid substratum (e.g., collagen), whereas in the latter cells are "sandwiched" by an additional layer of either collagen or a composite of extracellular matrix proteins, such as Matrigel ( LeCluyse, 2001 ). (aspetjournals.org)
The aim of this thesis is to investigate how the turnover (synthesis/breakdown) of cartilage extracellular matrix proteins responds to selected pro-inflammatory cytokines, thereby, gaining knowledge about cartilage biology, function and pathology. (lu.se)

Under HG conditions, reactive oxygen species release from monocytes was not inhibited by agents affecting mitochondrial metabolism but was inhibited in human endothelial cells. (diabetesjournals.org)
Changes were observed in the expression of proteins involved in metabolism, chromatin modification, post-transcriptional modification, and regulation of the transcription factor, c-Myc. (uwo.ca)
We also found that interleukin-17A can upregulate other pro-inflammatory cytokine release from chondrocytes and a release of both proteins and protein metabolism fragments into conditioned media. (lu.se)

Adhesion of MSCs to DLT molded in silicone and particularly in collagen initiates polygonal morphology and monolayer formation and enhances not only transcription of CEC typical genes such as ZO-1, Na/K-ATPase, PITX2, and COL-8 but also expression of the corresponding proteins. (lww.com)
The draft genome was queried and five potential genes were found that would encode for a heme containing protein in the 35-kda size range. (nasa.gov)
One of these genes is of particular interest, a di-heme cytochrome c peroxidase, occurs in an gene cluster with five other genes which encode for other proteins involved in electron transport. (nasa.gov)
Primers were developed for these six genes for use in RT-PCR to determine if any of these genes are up-regulated under Fe-replete conditions. (nasa.gov)
Understanding which proteins are involved in Fe-oxidation, and the genes that encode for their production could lead ultimately to an RNA or antibody assay for environmental studies. (nasa.gov)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

Conditioned media from the CBox exposed macrophage cultures was used to treat mouse endothelial cells (C166). (cdc.gov)
Moreover, we found that glioblastoma cells, notorious for their VEGF production, elicited a similar response when co-cultured with endothelial cells [11] . (plos.org)

To inactivate recombinant trypsin, a soybean trypsin inhibitor (STI) should be added to the medium. (researchsquare.com)
Recombinant Lucia Luciferase Protein is a positive control for luciferase reporter assays. (invivogen.com)
A dilution series of the recombinant Lucia luciferase protein can also be used to determine the linear range of the assay. (invivogen.com)
Activity of recombinant Lucia luciferase protein determined by measuring Relative Light Units (RLU) in a luminometer using QUANTI-Luc™ . (invivogen.com)
Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)

Centromeric heterochromatin formation was augmented upon transfer to the rich medium in wild-type cells. (nih.gov)
A needle is inserted into the back of the patients hip bone and cells are removed to be grown under controlled in vitro conditions in a lab. (wikipedia.org)
An issue related to culturing mesenchymal stem cells is the insufficient number of cells that can be produced. (wikipedia.org)
During long-term culture, mesenchymal stem cells age, lose their ability to differentiate, and have a higher chance to undergo malignant transformation. (wikipedia.org)
Genetic mutations in stem cells as well as conditions at target tissue may result in formation of a cancerous tumor. (wikipedia.org)
O 2 − release, protein kinase C (PKC) activity, and translocation of PKC-α and -βII and p47phox were increased in THP-1 cells (human monocytic cell line) under HG (15 mmol/l glucose) conditions, whereas AT supplementation inhibited these changes. (diabetesjournals.org)
PKC activity is increased in retina, aorta, heart, and renal glomeruli of diabetic rats as well as in cultured vascular cells or tissues exposed to HG conditions ( 6 ). (diabetesjournals.org)
Recent studies indicate that nutrient fluctuations and insulin resistance increase proinsulin synthesis in β cells beyond the capacity for folding of nascent polypeptides within the endoplasmic reticulum (ER) lumen, thereby disrupting ER homeostasis and triggering the unfolded protein response (UPR). (jci.org)
These findings suggest that CHOP is a fundamental factor that links protein misfolding in the ER to oxidative stress and apoptosis in β cells under conditions of increased insulin demand. (jci.org)
In conclusion, Vero cells were grown on Cytodex 1 with serum-free media and a high amount of rabies virus was obtained. (researchsquare.com)
Adherent Vero cells can be used in different kinds of microcarriers for industrial production in cell culture type bioreactors. (researchsquare.com)
This monomeric protein is expressed in CHO cells. (invivogen.com)
Additionally, saikosaponins A and D inhibited mRNA expression of parathyroid hormone-related protein as well as cell viability and invasion in metastatic human breast cancer cells. (hindawi.com)
Results Strong hypoxia-inducible factor-2 protein expression was detected in CD138 + multiple myeloma plasma cells in patients' biopsy specimens. (haematologica.org)
This technique requires that whole proteins or selected peptide antigens are added to blood cells, allowing the simultaneous analysis of both major histocompatibility complex class I and II restricted T-cell responses ( 7 ). (cdc.gov)
PBMC were cultured in complete Roswell Park Memorial Institute 1640 medium, 10% v/v heat-inactivated fetal calf serum, 2 mM L-glutamine, and 10 U/mL penicillin/streptomycin at a concentration of 10 6 cells/mL. (cdc.gov)
Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
F urther complicating matters, human ES cells are typically co-cultured with feeder layers of mouse fibroblast cells. (ddw-online.com)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
Several growth factors have been identified that promote growth of human ES cells in culture, most notably basic fibroblast growth factor (bFGF). (ddw-online.com)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
Xeno-free media that do not rely upon high levels of bFGF have been developed that contain only humanised or synthetic components and are engineered to support the undifferentiated growth and expansion of human ES cells. (ddw-online.com)
Use of progenitor cell lines can eliminate the need to culture challenging human ES cells. (ddw-online.com)
Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
Identifying the right cocktail of media conditions, supplements and growth factors that successfully drive stem cells toward a desired lineage on a reproducible basis is a time-consuming, iterative exercise. (ddw-online.com)
TNT cellular networks are upregulated between cancer and stromal cells under hypoxic and other conditions of physiologic and metabolic stress. (frontiersin.org)
iPS cells were cultured in a medium without glucose and supplemented with galactose, as well as 1 mM of calcium lactate, sodium lactate or lactic acid. (spandidos-publications.com)
After 7 days of culture, the cells were subjected to reverse transcription‑quantitative PCR analysis. (spandidos-publications.com)
The galactose‑1‑phosphate concentration was significantly higher in cells cultured in HDI than in those cultured with ReproFF. (spandidos-publications.com)
It was suggested that lactate promoted the survival of iPS cells cultured in a medium without glucose and supplemented with galactose. (spandidos-publications.com)
Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
Cells die in glucose-deprived media ( 16 ). (spandidos-publications.com)
A Hepatocyte selection medium (HSM) was developed to enrich hepatocytes from co-cultures with iPS cells ( 18 ). (spandidos-publications.com)
Prof. Dr. Paul Honegger and his group at the University of Lausanne developed aggregating cultures of mechanically dissociated primary fetal brain cells which provide an excellent system for neurobiological studies of cellular growth and differentiation. (forschung3r.ch)
The latter limits the choice of cell culture models, possibly excluding cultures of normal, untransformed cells, which are the most interesting and versatile for basic research. (forschung3r.ch)
The further identification of serum components that are obviously required by cultured cells is of less high priority. (forschung3r.ch)
Of all the components taken into account for cultured cells, the lipids are the most neglected ones. (forschung3r.ch)
This 3D primary cell culture system prepared from embryonic rat brain cells (2-5) contains all types of brain cells (i.e., neurons, astrocytes, oligodendrocytes, and microglia) that are enabled to grow and mature in serum-free medium, and to form highly differentiated structures such as myelinated axons, synapses, and functional neuronal networks. (forschung3r.ch)
GDNF production in individual cells in the culture was analyzed by Immunocytochemistry. (sld.cu)
Cell culture process development begins with cell line generation which typically consists of a common host cell such as HEK 293 or CHO cells, an expression or infection system and selection, followed by process and media optimization in small scale systems such as 96-well plates or bench-scale bioreactors. (beckman.com)
These small highly conserved actin-binding proteins are essential regulators of actin dynamics in living cells. (rupress.org)
But in some cases, red or white blood cells in your urine may mean that you have a medical condition that needs treatment, such as a urinary tract infection (UTI) , kidney disease , or liver disease . (medlineplus.gov)
Researchers from Lund have developed a completely animal-free preparation for cell culture, a medium that can be used to grow human cells, including for cancer research. (lu.se)
Scientists also added various protein extracts from animals to make the cells grow. (lu.se)
We show that it is possible to grow cells successfully with a completely defined and animal product-free medium," says Stina Oredsson. (lu.se)
Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)

Peroxidase activity assay shows this protein contains heme. (nasa.gov)
Assay conditions, such as culture format and duration, media supplements etc., were reported previously to alter the phenotype and induction response of cultured hepatocytes ( LeCluyse, 2001 ). (aspetjournals.org)

lcn2 expression and secretion increased after inflammatory stimulation in cultured astrocytes. (jneurosci.org)
The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
Fibrogenic particulates induced high levels of IL-1beta mRNA expression and protein secretion that are required for macrophage-guided myofibroblast transformation. (cdc.gov)

Although this approach eliminates the need for feeder layers, the system still contains undefined animal proteins, as well as possible contaminants. (ddw-online.com)
It has worked very well, despite both animal ethics and scientific concerns - which is why no one has really tackled developing a medium with defined content and without animal proteins. (lu.se)

Marinobacter aquaeolei , a litho-heterotroph that is closely related to many deep-sea, Fe(II)-oxidizing autotrophic bacteria, to examine a potential mechanism of Fe-oxidation by tracking changes in protein expression resulting from changing Fe concentrations. (nasa.gov)
To gain insight into this mechanism, we used mass spectrometry to investigate changes in protein expression in hESCs cultured in hypoxia. (uwo.ca)

a protein found in the blood that is produced in response to foreign substances (e.g., bacteria or viruses) invading the body. (cdc.gov)
A urinalysis also measures other substances in the urine, including proteins, acid and sugar levels, cell fragments, and crystals. (medlineplus.gov)

They are capable of secreting a broad range of biomolecules such as proteins, nucleic acids, exosomes, microRNAs, and membrane vesicles, collectively known as secretomes. (techscience.com)

Here, we report the use of a targeted liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) method for concurrent quantification of multiple cytochrome P450 (P450), UDP-glucuronosyltransferase (UGT), and transporter proteins in cultured primary human hepatocytes. (aspetjournals.org)
In general, P450 and UGT expression was lower in hepatocyte cultures than that in liver, and CYP2C9 was found to be the most abundant P450 isoform expressed in cultured hepatocytes. (aspetjournals.org)
The sandwich culture format and 0.1 μM DEX in media retained the protein expression in the hepatocytes closest to the levels found in liver. (aspetjournals.org)
Direct protein quantification was applied successfully to study in vitro induction in sandwich cultured primary hepatocytes in a 24-well format using the prototypical inducers rifampicin, omeprazole, and phenobarbital. (aspetjournals.org)
Cultured hepatocytes offer the possibility to assess long-term effects, such as enzyme induction (i.e., changes of the expression levels of molecular entities of the hepatocytes). (aspetjournals.org)

He had a single propagated in RPMI 1640 medium supple- lesion at the inoculation site which had not mented with 15% fetal calf serum and L- healed in spite of extensive treatment for 13 glutamine. (who.int)

After two days of culture in HSM, the expression levels of α-fetoprotein (AFP) and albumin, which are hepatocyte markers, were upregulated, suggesting that hepatocyte differentiation was initiated ( 19 ). (spandidos-publications.com)
Then, combine the ECM solution, the 2%thiolated hyaluronic acid, the 2%thiolated gelatin, the crosslinkers, and hepatocyte culture media in the ratios shown here. (jove.com)

Macrophage-conditioned media was collected and applied to cultures of human pulmonary fibroblast line WI38-VA13 to induce myofibroblast transformation. (cdc.gov)

The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
We then determined the protein cargo in the EV-enriched secreted fractions of T. crassiceps and M. corti conditioned media by LC-MS/MS. Among the identified proteins, eukaryotic vesicle-enriched proteins were identified as expected, but also proteins used for cestode disease diagnosis, proteins related to neurotransmission, lipid binding proteins as well as host immunoglobulins and complement factors. (conicet.gov.ar)

hESCs in feeder free culture were incubated in 1% oxygen or 20% oxygen for 48 and 72 hours. (uwo.ca)

Diagnosis requires synovial fluid analysis and culture. (msdmanuals.com)

The resulting duplication of the lnp1(+) gene, which encodes an endoplasmic reticulum membrane protein, suppressed lem2∆ phenotypes, whereas the lem2∆ lnp1∆ double mutant showed a severe growth defect. (nih.gov)
A combination of mutations in Lem2 and Bqt4, which encodes a nuclear membrane protein that anchors telomeres to the nuclear membrane, caused synthetic lethality. (nih.gov)

The allelopathic potential of extracts of Azadirachta indica L., which is one of the mostdominant weeds in tropical regions of South-west Asia, was investigated under laboratory conditions. (researchgate.net)

Therefore, selection of the appropriate culture format and conditions for a specific in vitro study is crucial with regard to the in vivo predictability of the data generated. (aspetjournals.org)

To meet volume demands, biopharmaceutical companies have built large-scale facilities with multiple 10,000 L (or even larger) cell culture bioreactors. (beckman.com)

Design and Methods The expression of hypoxia-inducible factor protein was examined in patients' bone marrow biopsy specimens using immunohistochemistry. (haematologica.org)
Promoter analyses revealed increased hypoxia-inducible factor-2 binding to the CXCL12 promoter under hypoxic conditions. (haematologica.org)
The mixture of conditions in the microenvironment exemplified by hypoxia-induced ovarian cancer TNTs playing a crucial role in tumor growth, as just one example, is a potential avenue of investigation that will uncover their role in relation to other known factors, including EVs. (frontiersin.org)
Immunofluroescence localization of Oct-4 revealed that cultures incubated in hypoxia were less differentiated than cultures incubated in normoxia. (uwo.ca)

In Paper I, we showed that miR-96 can upregulate the mRNA and protein expression of adhesion proteins E-Cadherin and EpCAM through direct interaction with target sites in the mRNA coding sequence. (lu.se)

LB Broth (Lennox) has been used for culturing Escherichia coli and bacterial artificial chromosomes (BACs). (sigmaaldrich.com)
In this study, batch cultures of the bacterium were initiated in artificial seawater medium with various concentrations of Fe or "biologically unavailable" ferrozine chelated Fe. (nasa.gov)

Chronic ER stress promotes apoptosis, at least in part through the UPR-induced transcription factor C/EBP homologous protein (CHOP). (jci.org)

Our results suggest that lcn2 acts in an autocrine manner to induce cell death sensitization and morphological changes in astrocytes under inflammatory conditions and that these phenotypic changes may be the basis of reactive astrocytosis in vivo . (jneurosci.org)
In the adult brain, the lack of these proteins can induce apoptotic neuronal death (7, 8). (sld.cu)

Once conditions are optimized and defined, the process typically transitions to a scalability pilot study followed by preclinical toxicology studies and ultimately scale-up to manufacture at commercial volumes. (beckman.com)
sentences are usually mal to fight shop національне питання and at Typically more at disease of dumping condition dermoid. (shotglass.org)

Use of human feeders derived under xeno-free conditions can maintain the xeno-free nature of the culture system and allow for the use of low levels of bFGF. (ddw-online.com)
But in 2018, researchers in Lund started using different human proteins needed for cell survival in a cell culture medium. (lu.se)

Fluorophores for STORM and SMLM include synthetic dyes, fluorescent proteins (FPs), and even Quantum Dots (QDots). (microscopyu.com)

Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)

Cell culture methods often lead to the reduction of animals used for biological research and industrial applications. (forschung3r.ch)
Using cell culture, animal and patient studies, we investigated the biological role of microRNA-96 (miR-96) and microRNA-379 (miR-379) in prostate cancer bone metastasis. (lu.se)

We found that shRNA knockdown of intrinsic ST6GAL1 expression resulted in decreased ST6GAL1 cargo in the exosome-like vesicles as well as decreased breast tumor cell growth and invasive behavior in 3D in vitro cultures. (nature.com)
Interestingly, while T. crassiceps and M. corti metacestodes secrete membranous structures into the culture media, similar vesicles were observed in the interface of the germinal and laminated layers of E. multilocularis metacestodes and were hardly detected in culture supernatants. (conicet.gov.ar)

Most of these cell culture systems rely on the use of fetal bovine serum (FBS), a supplement of undefined and variable composition. (forschung3r.ch)

Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)
The third is by the addition of additives such as proteins or growth hormones to the culture medium to promote growth. (wikipedia.org)
Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli . (sigmaaldrich.com)
LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture. (sigmaaldrich.com)
Another is to adapt useful cell lines individually for survival and growth in serum-free culture media. (forschung3r.ch)
Previous work showed that this culture system was ideal for the study of hormones, growth factors and other signaling molecules on cellular growth, maturation and homeostasis. (forschung3r.ch)
Producing highly productive cell lines and optimized cell culture conditions requires an understanding of the contributions of many key variables: cell culture media, cell growth rates, cell viability, protein expression levels, and other quality attributes including size, aggregation status, glycosylation and stability. (beckman.com)
Predictive microbiology uses mathematical models to predict the growth of bacteria based on environmental conditions. (bvsalud.org)
Downregulation of miR-379 enhanced incidence of bone metastasis in mice, increased colony formation potential in osteoblast-conditioned media, and increased cell growth. (lu.se)

LCN2 protein induced upregulation of glial fibrillary acidic protein (GFAP), cell migration, and morphological changes similar to characteristic phenotypic changes termed reactive astrocytosis. (jneurosci.org)

All cell culture conditions and virus growing kinetics in serum-free media were investigated. (researchsquare.com)
The cell culture-based rabies vaccine was established in 1967. (researchsquare.com)
The ultimate goal remains to find a chemically defined culture medium appropriate for a broad range of cell culture systems. (forschung3r.ch)
Are lipids essential for cell culture? (forschung3r.ch)
Knowing that lipids, and particularly cholesterol, are absent from most conventional culture media, the working hypothesis of the present investigation was that the LPs represented the still undefined serum component(s) required for cell culture. (forschung3r.ch)
When the final cell culture process is established, companies move forward with the commercialization process which includes process characterization, scale-up, technology transfer and manufacturing process validation. (beckman.com)
She has developed an animal-free preparation for cell culture - and is giving away the recipe! (lu.se)
Cell culture was initiated in the early 20th century with simple salt solutions. (lu.se)
Adding FBS to a cell culture medium is like working with a black hole. (lu.se)

Fermented alt-seafood company Aqua Cultured Foods says it has successfully doubled it outputs after overhauling its fermentation technology. (thefishsite.com)
Food tech startup Aqua Cultured Foods announced that it has successfully made advancements in its novel fermentation technology that doubles its biomass output in the same time frame. (thefishsite.com)

Unlike plant-based processed foods formulated with starches and protein isolates, products made from microbial fermentation retain naturally occurring fibre, protein and other micronutrients. (thefishsite.com)

Cell infection, at an MOI of 0.3 with serum-free medium conditions, yielded a maximal rabies virus titer of 1.82×10 7 FFU/mL at 5 days. (researchsquare.com)
In his present investigation he used this serum-free aggregating brain cell cultures as a reference culture system. (forschung3r.ch)
In the present project (No 109-08 ) the utility of blood serum or plasma proteins for FBS replacement was investigated. (forschung3r.ch)
To avoid the use of serum in culture media, two approaches are currently being employed. (forschung3r.ch)

The effects of culture format (i.e., sandwich culture versus conventional culture) and of dexamethasone (DEX) media concentrations on mRNA, protein, and activity levels were determined for three donors, and protein expression was compared with that in liver. (aspetjournals.org)

Inner nuclear membrane proteins interact with chromosomes in the nucleus and are important for chromosome activity. (nih.gov)
Lem2 and Man1 are conserved members of the LEM-domain nuclear membrane protein family. (nih.gov)
These genetic interactions imply that Lem2 cooperates with the nuclear membrane protein network to regulate genome stability. (nih.gov)
Protein extractions from these incubations show the presence of a circa 35-kDa protein located in the cell membrane in the Fe replete conditions. (nasa.gov)

2. Virkon and tissue culture hood with UV-light. (lu.se)
3. 15-cm tissue culture-treated plastic dishes. (lu.se)

Since cholesterol is practically insoluble in aqueous solutions, it is generally absent from culture media. (forschung3r.ch)

Aqua's techniques for "growing" protein through microbial fermentation rely on manipulating a series of environmental factors such as ingredients in its culture medium, temperature, humidity, and oxygen levels. (thefishsite.com)

Under healthy physiological conditions, activities of osteoclasts and osteoblasts are tightly controlled by both systemic and local factors, maintaining bone mineral density and bone microarchitecture. (hindawi.com)

Anatomy38

Organisms24

Diseases2

Chemicals and Drugs89

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes48

Disciplines and Occupations4

Technology, Industry, Agriculture4

Information Science7

Health Care6

Production of recombinant proteins1

Extracellular4

Metabolism3

Genes6

Endothelial cells2

Recombinant5

Cells46

Assay2

Secretion3

Animal proteins2

Changes in protein expression2

Substances2

MicroRNAs1

Hepatocytes5

1640 medium1

Hepatocyte2

Fibroblast1

Eukaryotic2

Feeder-free1

Diagnosis1

Encodes2

Extracts1

Vitro study1

Bioreactors1

Hypoxia4

Adhesion1

Artificial2

Apoptosis1

Induce2

Typically2

Human2

Synthetic1

Stem1

Biological2

Vesicles2

Fetal1

Growth9

Upregulation1

Cell culture9

Successfully2

Isolates1

Serum4

Concentrations1

Membrane4

Tissue culture2

Aqueous solutions1

Temperature1

Bone1