Loading...
  • structural
  • The technique is shown to have some advantages over differential scanning calorimetry for the study of structural change, and can also be used to measure the affinity of carbohydrate binding proteins, such as Lectin, with monosaccharides. (technologynetworks.com)
  • Is the Subject Area "Structural proteins" applicable to this article? (plos.org)
  • Are you looking for a way to identify structural hotspots within your potential biotherapeutic protein? (bruker.com)
  • Clemmer's work on gas-phase separation methods for ion mobility-mass spectrometry (IM-MS) and their application to the structural analysis of intact proteins is considered a "particularly important milestone" in the application of IM-MS to the examination of biomolecular structures. (wikipedia.org)
  • Examples of structural inheritance include the propagation of prions, the infectious proteins of diseases such as scrapie (in sheep and goats), bovine spongiform encephalopathy ('mad cow disease') and Creutzfeldt-Jakob disease (although the protein-only hypothesis of prion transmission has been considered contentious until recently). (wikipedia.org)
  • amino acids
  • After two German chemists, Emil Fischer and Franz Hofmeister, independently stated in 1902 that proteins are essentially polypeptides consisting of many amino acids , an attempt was made to classify proteins according to their chemical and physical properties, because the biological function of proteins had not yet been established. (britannica.com)
  • The problem becomes even more challenging due to the fact that a typical protein can contain several hundreds of amino acids or several thousands of atoms. (biomedcentral.com)
  • From these frequencies a set of probability parameters were derived for the appearance of each amino acid in each secondary structure type, and these parameters are used to predict the probability that a given sequence of amino acids would form a helix, a beta strand, or a turn in a protein. (wikipedia.org)
  • by contrast, the large aromatic residues (tryptophan, tyrosine and phenylalanine) and Cβ-branched amino acids (isoleucine, valine, and threonine) prefer to adopt β-strand conformations. (wikipedia.org)
  • The unmodified protein has 358 amino acids, a predicted molecular weight of 40kDa, a charge of -11, and an isoelectric point of 5. (wikipedia.org)
  • The human alpha-synuclein protein is made of 140 amino acids and is encoded by the SNCA gene. (wikipedia.org)
  • The majority form of the protein, and the one most investigated, is the full-length protein of 140 amino acids. (wikipedia.org)
  • alpha helices
  • The method is based on analyses of the relative frequencies of each amino acid in alpha helices, beta sheets, and turns based on known protein structures solved with X-ray crystallography. (wikipedia.org)
  • The highly conserved and slowly evolving DUFF3314 region is predicted to form numerous alpha helices and may be vital to the function of the protein. (wikipedia.org)
  • A cross-program consensus predicted that UPF0575 protein C19orf67 forms four alpha helices and two beta sheets in the following locations in the amino acid sequence: Acetylation is likely to occur at the N-terminus while the C-terminus is unlikely to be modified. (wikipedia.org)
  • molecule
  • Proteins, when forming an interfacial film, are present as a monomolecular layer-i.e., a layer one molecule in height. (britannica.com)
  • The application of lateral pressure on a protein film causes it to increase in thickness and finally to form a layer with a height corresponding to the diameter of the native protein molecule. (britannica.com)
  • Experimental techniques are able to estimate distances between pairs of atoms of a given molecule, and the problem becomes the one of identifying the three-dimensional conformation of the molecule, i.e. the positions of all its atoms. (wikipedia.org)
  • crystallography
  • Leading experts in the fields of NMR spectroscopy, X-ray crystallography, protein engineering and molecular modeling offer provocative insights into current views on the protein folding problem and various aspects for future progress. (wiley.com)
  • Similar arguments, such as protein confinement by the crystal lattice and using cryogenic temperatures for collecting diffraction pattern could be (and are) applied to X-ray crystallography. (grantome.com)
  • Dynamic information from protein crystallography. (wikipedia.org)
  • interactions
  • From an inventory of interactions derived from the x-ray crystal structure of a protein, the stabilizing contribution of each amino acid be calculated using the free energies determined from thermodynamic studies of model systems. (springer.com)
  • Research in my lab focuses on atomic-level mechanisms of protein regulation, protein-ligand interactions, and computer-aided drug design. (ucsf.edu)
  • Do you want to understand more about the protein-ligand (drug/protein) interactions and their relationship to drug activity? (bruker.com)
  • Researchers within (bio)pharmaceutical sciences are increasingly tasked with gaining a deeper knowledge and understanding of the effects interactions and stressors such as pH or temperature, have on the conformational structure of biotherapeutic proteins. (bruker.com)
  • This is a very challenging task and researchers often use a variety of analytical tools to study the higher order structure of the biotherapeutics, monitor protein/protein-drug interactions and perform epitope mapping. (bruker.com)
  • particles
  • We characterized recombinant Env proteins and virus particles produced in mammalian cells that lack N-acetylglucosaminyltransferase I (GnTI), an enzyme necessary for the conversion of oligomannose N-glycans to complex N-glycans. (iavi.org)
  • There are two major proteins on the surface of influenza virus particles. (wikipedia.org)
  • structures
  • Comparative Analysis of Protein Three-Dimensional Structures and an Approach to the Inverse Folding Problem (T. Blundell). (wiley.com)
  • To that end, this reference sheds light on the methods used for protein structure prediction and reveals the key applications of modeled structures. (acronymfinder.com)
  • The Chou-Fasman method is an empirical technique for the prediction of secondary structures in proteins, originally developed in the 1970s by Peter Y. Chou and Gerald D. Fasman. (wikipedia.org)
  • The original Chou-Fasman parameters were derived from a very small and non-representative sample of protein structures due to the small number of such structures that were known at the time of their original work. (wikipedia.org)
  • Other extended structures such as the polyproline helix and alpha sheet are rare in native state proteins but are often hypothesized as important protein folding intermediates. (wikipedia.org)
  • Low frequency collective vibrations are thought to be sensitive to local rigidity within proteins, revealing beta structures to be generically more rigid than alpha or disordered proteins. (wikipedia.org)
  • Within these structures, alpha-synuclein interacts with phospholipids and proteins. (wikipedia.org)
  • They identified that the beta-alpha-beta unit in proteins is nearly always right handed and this explained remarkable similarities between protein structures. (wikipedia.org)
  • yeast
  • Recent emergence of oseltamivir and zanamivir resistant human influenza A(H1N1) H274Y has emphasized the need for suitable expression systems to obtain large quantities of highly pure and stable, recombinant neuraminidase through two separate artificial tetramerization domains that facilitate the formation of catalytically active neuraminidase homotetramers from yeast and Staphylothermus marinus, which allow for secretion of FLAG-tagged proteins and further purification. (wikipedia.org)
  • Prions based on heritable protein structure also exist in yeast. (wikipedia.org)
  • vitro
  • Proteins are commonly the first biomolecules that NPs encounter when they interact with biological systems either in vitro or in vivo . (icmab.es)
  • interacts
  • We believe that a complete understanding of the behavior and morphology of BSA-SPIONs and how the protein interacts with SPIONs is crucial for improving NP surface design and expanding the potential applications of SPIONs in nanomedicine. (icmab.es)
  • It has been shown that alpha-synuclein significantly interacts with tubulin, and that alpha-synuclein may have activity as a potential microtubule-associated protein, like tau. (wikipedia.org)
  • Hydrogen
  • In recent years Hydrogen Deuterium Exchange Mass Spectrometry (HDX-MS) has emerged as a valuable technique within the arsenal of analytical tools available for providing such insights into protein structure conformation. (bruker.com)
  • Other helices, such as the 310 helix and π helix, are calculated to have energetically favorable hydrogen-bonding patterns but are rarely observed in natural proteins except at the ends of α helices due to unfavorable backbone packing in the center of the helix. (wikipedia.org)
  • T = hydrogen bonded turn (3, 4 or 5 turn) E = extended strand in parallel and/or anti-parallel β-sheet conformation. (wikipedia.org)
  • scrapie
  • The protein can exist in multiple isoforms, the normal PrPC, and as Protease resistant PrPRes like the disease-causing PrPSc(scrapie) and an isoform located in mitochondria. (wikipedia.org)
  • tissues
  • The protein product is less abundant than most of the human proteome in many tissues. (wikipedia.org)
  • Expression of the protein is most predominant in the nervous system but occurs in many other tissues throughout the body. (wikipedia.org)
  • however
  • However, large proteins present special challenges. (rice.edu)
  • However, it is not well understood to what extent protein conformations represented by these distributions correspond to conformations in solution. (grantome.com)
  • However, for a folded protein or a protein complex the picture may be more complex, since many bonds allow for substantial freedom and may produce an ensemble of conformations that exchange slow and can get trapped. (grantome.com)
  • The proline content by percentage was found to be higher in UPF0575 than 95% of comparable human proteins.However, the amount of asparagine the protein is less abundant when compared to the human proteome. (wikipedia.org)
  • pathways
  • Folding versus aggregation: polypeptide conformations on competing pathways. (springer.com)
  • CAVER 3.0: a tool for the analysis of transport pathways in dynamic protein structures. (nih.gov)
  • Characteristics of individual transport pathways, including their geometry, physico-chemical properties and dynamics are instrumental for understanding of structure-function relationships of these proteins, for the design of new inhibitors and construction of improved biocatalysts. (nih.gov)
  • Arrestin binding to the receptor blocks further G protein-mediated signaling and targets receptors for internalization, and redirects signaling to alternative G protein-independent pathways, such as β-arrestin signaling. (wikipedia.org)
  • Mitochondria
  • In the cells of extant organisms, the vast majority of the proteins present in the mitochondria (numbering approximately 1500 different types in mammals) are coded for by nuclear DNA, but the genes for some, if not most, of them are thought to have originally been of bacterial origin, having since been transferred to the eukaryotic nucleus during evolution. (wikipedia.org)
  • regulate
  • Bacterial origins regulate orisome assembly, a nuclei-protein complex assembled on the origin responsible for unwinding the origin and loading all the replication machinery. (wikipedia.org)
  • A few other mechanisms in E. coli that variously regulate initiation are DDAH (datA-Dependent DnaA Hydrolysis, which is also regulated by IHF), inhibition of the dnaA gene (by the SeqA protein), and reactivation of DnaA by the lipid membrane. (wikipedia.org)
  • secondary structure
  • Short coding motifs, which appear to lack secondary structure, include those that label proteins for delivery to particular parts of a cell, or mark them for phosphorylation. (wikipedia.org)
  • A polyproline helix is a type of protein secondary structure which occurs in proteins comprising repeating proline residues. (wikipedia.org)
  • genome
  • With full-genome sequences available, structure prediction can be done more quickly through a combination of experimental and modeling approaches, especially because the availability of large number of sequenced genomes and previously solved protein structures allows scientists to model protein structure on the structures of previously solved homologs. (wikipedia.org)
  • Genes
  • In facultative aerobes the bacterial members of this family encode a single domain as a stand-alone protein, and genes that encode for either putative histidine kinases or diguanylate cyclases are found in the same predicted operon. (alusdomundo.com)
  • These vesicles are complex structures made of proteins encoded by at least 14 genes. (wikipedia.org)
  • coli
  • Since protein expression in Escherichia coli represents the most facile approach (Mahalik et al. (springeropen.com)
  • The T4 phage initiates an Escherichia coli infection by binding OmpC porin proteins and Lipopolysaccharide (LPS) on the surface of E. coli cells with its long tail fibers (LTF). (wikipedia.org)
  • Annotation
  • p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc. (uniprot.org)
  • Domains
  • This protein belongs to the LDLR family and is made up of a number of functionally distinct domains, including 3 EGF-like domains, 7 LDL-R class A domains, and 6 LDL-R class B repeats. (wikipedia.org)
  • Like other GT-B proteins, SPS contains two Rossman fold domains that are named the A domain and the B domain. (wikipedia.org)
  • this binding is important for many protein-protein interactions and even for interactions between the domains of a single protein. (wikipedia.org)
  • sequences
  • Noncoding" sequences are not translated into proteins, and nucleic acids with such motifs need not deviate from the typical shape (e.g. the "B-form" DNA double helix). (wikipedia.org)
  • Promoters contain specific DNA sequences such as response elements that provide a secure initial binding site for RNA polymerase and for proteins called transcription factors that recruit RNA polymerase. (wikipedia.org)
  • folds
  • Experimental methods of protein structure determination require proteins that express and/or crystallize well, which may inherently bias the kinds of proteins folds that this experimental data elucidate. (wikipedia.org)
  • A genomic, modeling-based approach such as ab initio modeling may be better able to identify novel protein folds than the experimental approaches because they are not limited by experimental constraints. (wikipedia.org)
  • cleavage
  • A typical TAP tag consists of the calmodulin-binding protein (CBP), a tobacco etch virus (TEV) protease cleavage site, and the immunoglobulin G-binding (IgG) units of protein A (ProtA) of Staphylococcus aureus (Fig. 2B). (pharmacologicalsciences.us)
  • mainly
  • This structure is somewhat similar to that adopted in the fibrous protein collagen, which is composed mainly of proline, hydroxyproline, and glycine. (wikipedia.org)
  • mechanism
  • Arrestins were first discovered as a part of a conserved two-step mechanism for regulating the activity of G protein-coupled receptors (GPCRs) in the visual rhodopsin system by Hermann Kühn, Scott Hall, and Ursula Wilden and in the β-adrenergic system by Martin J. Lohse and co-workers. (wikipedia.org)
  • Images of an aerolysin oligomer derived from electron microscopy and molecular dynamics simulations have helped to construct a model of the protein in its heptameric conformation, and to outline a mechanism by which this assembly might insert into lipid bilayers to form ion channels. (wikipedia.org)
  • poorly
  • However, at a common temperature of measurement, the higher configurational entropy of a cold-adapted enzyme may foster conformations that bind ligands poorly, leading to high Km values relative to warm-adapted orthologs. (antibody-antibodies.com)
  • important
  • This chapter describes the current research regarding polyQ misfolding, with emphasis on the species populated during aggregation, suggesting an important role of protein context in modulating the aggregation pathway. (springer.com)
  • Arrestins (abbreviated Arr) are a small family of proteins important for regulating signal transduction at G protein-coupled receptors. (wikipedia.org)
  • methods
  • We facilitate antibody generation for our clients by: consulting with our clients to find the best and most cost effective methods to meet their needs, generating of protein Immunogens using a number of expression systems, design custom protein immunogens to produced antibodies against linear as well as conformational epitopes. (ichorbiologics.com)
  • highly
  • Bhattacharyya AM, Thakur AK, Wetzel R. polyglutamine aggregation nucleation: thermodynamics of a highly unfavorable protein folding reaction. (springer.com)
  • These highly acidic proteins are overwhelmingly negative in charge and are able to remain in solution even at high salt concentrations. (wikipedia.org)
  • order
  • These octomers do not form higher order oligomers, and three of the eight proteins are glycosylated themselves. (wikipedia.org)