• The open reading frame of the Zika virus reads as follows: 5′-C-prM-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-N​S5-3′ and codes for a polyprotein that is subsequently cleaved into capsid (C), precursor membrane (prM), envelope (E), and non-structural proteins (NS). (kenyon.edu)
  • It is then translated into a polyprotein that is subsequently cleaved to form all structural and non-structural proteins. (kenyon.edu)
  • Each RNA encoded a single polyprotein, flanked by the 5′ and 3′ non-coding regions (NCR) and followed by a poly (A) tail. (springer.com)
  • The 5'-UTR is the main target detected in nucleic acid-based assays, while serologic assays detect HCV antibodies directed against peptides c22-3, c33c, and c100-3 that are derived from the various viral proteins. (aacc.org)
  • Of the structural proteins, GP2, GP3, GP4, and GP5 are glycosylated and present on the viral envelope, along with the nonglycosylated M and E proteins. (hindawi.com)
  • The two G-protein coupled receptors GPR40 (Ffar1) and GPR120 are activated by medium and long chain fatty acids. (jneurosci.org)
  • For sweet, bitter and umami, the taste sensation is initiated by tastants binding to G-protein coupled receptors (for review see Lindemann, 2001 ). (jneurosci.org)
  • First, the virion attaches to the host cell membrane receptors via the envelope protein which induces virion endocytosis. (kenyon.edu)
  • The receptors, which are alternately spliced versions of MCP-1 receptor protein may be used in an assay to identify antagonists of MCP-1 which are therapeutically useful in the treatment of atherosclerosis. (google.com)
  • These proteins bind to the surface of ice crystals and prevent water molecules from binding to the ice crystals, preventing the ice crystals from growing. (creation.com)
  • Additionally, the EF1a-RFP-T2A-Puro cassette can be excised by transient expression of Cre recombinase, leaving only the GFP fusion protein and a single LoxP site. (biocat.com)
  • DIRAS3 is required for autophagy and that re-expression of DIRAS3 protein in ovarian cancer cells will induce autophagic cell death in culture. (atlasgeneticsoncology.org)
  • Oligosaccharide-Protein Conjugate Vaccines Induce and Prime for Oligoclonal IgC Antibody Responses to the Haemophilus influenzae by Capsular Polysaccharide in Human Infants",J. Exp. (patentgenius.com)
  • Genetic code is universal. (coursehero.com)
  • Heritable traits in the population likely explain why some smokers develop lung cancer ( 3 - 6 ), and the identification of genetic variants that regulate the activation and detoxification of tobacco smoke carcinogens is necessary to identify high-risk smokers and develop targeted interventions. (aacrjournals.org)
  • Apart from providing an optimized cloning strategy that generates fully exchangeable genetic elements for multigene engineering, the GB2.0 toolkit offers an ever-growing open collection of DNA parts, including a group of functionally tested, premade genetic modules to build frequently used modules like constitutive and inducible expression cassettes, endogenous gene silencing and protein-protein interaction tools, etc. (plantphysiol.org)
  • Trtikova M, Wikmark OG, Zemp N, Widmer A, Hilbeck A (2015) Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions. (springer.com)
  • The TrpGFP mice were made with a construct containing, 5′ to 3′, 11kb of mouse Trpm5 5′ flanking region, the coding region of enhanced green fluorescent protein (eGFP), an internal ribosome entry site (IRES) and a truncated human CD4 gene to be used in other studies as a surface marker of the cells that express the transgene. (jneurosci.org)
  • The transgene consists of the coding region for the herpes simplex virus-thymidine kinase (HSV-TK) under the control of the human glial fibrillary acidic protein gene promoter. (jneurosci.org)
  • This analysis extended the region of GLC1K from D20S846 to rs6081603 in affected individuals, and the region was further reduced to 9 Mb if unaffected recombinant individuals were included in the analysis. (molvis.org)
  • Using recombinant breakpoint mapping and haplotypes based on a combination of SNP and microsatellite markers, the GLC1K region has been reduced to a maximum of 12.7 Mb and a minimum of 9 Mb. (molvis.org)
  • The recombinant lipase (ReLipA) was secreted as an active protein with the highest activity of 1961 U mL −1 in a 5-L fermentor by high cell-density fermentation. (biomedcentral.com)
  • The present invention provides a recombinant DNA that is composed of a promoter, a protein coding region, and the hybrid 3′UTR in a continuous and directional orientation. (freepatentsonline.com)
  • 2. The recombinant nucleic acid of claim 1, wherein said first region and said second region are in proximity to each other or are adjacent to each other or overlap with each other or one encompasses the other. (freepatentsonline.com)
  • 3. The recombinant nucleic acid of claim 2, wherein the 3′ end of the first region is adjacent to the 5′ end of the second region or the 3′ end of the second region is adjacent to the 5′ end of the first region or the first region is located within the second region. (freepatentsonline.com)
  • The DIRAS3 gene ORF contains three motifs typical of Ras/Rap family members: (1) a highly conserved GTP binding domain, (2) a putative effector domain YLPTIENTY, and (3) the membrane localizing CAAX motif at the COOH terminus. (atlasgeneticsoncology.org)
  • 4 , 5 This achieved two fortuitous things that transformed the putative SAS gene into an AFP gene: it stripped it of any SAS function, and enabled a change in reading frame, which altered the signal peptide, enabling the new protein to be secreted. (creation.com)
  • 3. The method of claim 2 , wherein the MCP-1RA polypeptide comprises SEQ ID NO: 2. (google.com)
  • From the analysis of restriction maps of the Amy region in eight sibling species belonging to the Drosophila melanogaster species subgroup, we herein show that the patterns of duplication of the Amy gene are almost the same in all species. (genetics.org)
  • This means that concerted evolution occurred only in the Amy coding region and that differentiated evolution between the duplication occurred in the flanking regions. (genetics.org)
  • Researchers have recently posited a detailed evolutionary scenario for the evolution of an antifreeze protein from such a gene duplication event in a species of Antarctic eelpout (ray-finned fish), Lycodichthys dearborni . (creation.com)
  • This novel type of protein processing was confirmed by a nucleotide sequencing study together with amino acid sequencing of two PGA subunits. (nih.gov)
  • We thus conclude that adaptive protein evolution occurred in the lineage of D. erecta that is a "specialist" species for host plants and probably also occurs in the process of speciation in general. (genetics.org)
  • Epigenetic analysis revealed a low degree of methylation, making it difficult to associate the coding region variants with methylation status. (springer.com)
  • Translational efficiency is regulated by the length of the 3′ untranslated region," Molecular and Cellular Biology, 1996, vol. 16, No. 1, pp. 146-156. (freepatentsonline.com)
  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. (biology-online.org)
  • p>This section provides any useful information about the protein, mostly biological knowledge. (uniprot.org)
  • ] The E protein composes the majority of the virion surface and is involved with aspects of replication such as host cell binding and membrane fusion. (kenyon.edu)
  • The 3´ homology arm can be cloned into the MCS using Cold Fusion cloning or traditional cloning using the provided restriction sites. (biocat.com)
  • 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone is a potent and abundant procarcinogen found in tobacco smoke, and glucuronidation of its major metabolite, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), by UDP-glucuronosyltransferases (UGT) including UGT2B17 is an important mechanism for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone detoxification. (aacrjournals.org)
  • 2 So do these AFPs represent a neo-Darwinian mechanism producing a new protein? (creation.com)