Protein characterization protein stability. Medical search. Frequent questions

Develops and applies analytical methods to support product characterization, formulation development, and stability testing of mAbs, bispecifics and other modalities. (biospace.com)
The company also provides a variety of process development activities, including cell line optimization, analytical methods development and product characterization. (globenewswire.com)

The objective of the present study was to report the stability of novel antiviral drug, valganciclovir based on the information obtained from forced degradation studies. (jascoinc.com)

Sendai virus M protein is found in two distinct isoforms defined by monoclonal antibodies. (microbiologyresearch.org)
With over 10 years of experience producing monoclonal antibodies and recombinant proteins in batch, fed-batch and perfusion modes, Avid's services include cell banking, stability testing, clinical and commercial product manufacturing and purification, bulk packaging, final product filling and regulatory strategy, submission and support. (globenewswire.com)
Can DSC calorimetry gauge long‑term stability for monoclonal antibodies? (europeanpharmaceuticalreview.com)
Can calorimetry provide accurate estimates of long‑term stability for monoclonal antibodies? (europeanpharmaceuticalreview.com)
Differential scanning calorimetry (DSC) has been one of the most widely used techniques to characterise the temperature stability of monoclonal antibodies (mAbs) and provide important information in formulation optimisation. (europeanpharmaceuticalreview.com)
Close to 60% of biopharmaceuticals (such as enzymes, proteins and monoclonal antibodies) must be lyophilized as they are insufficiently stable for ready-to-use solution dosage forms. (pharmaceutical-technology.com)

Monitor the hydrodynamic properties of biomacromolecules and protein-protein interactions. (utmb.edu)
This information can be used to analyze protein-protein interactions, the presence of oligomeric states, and conformational rearrangement of molecules. (utmb.edu)
Recently we have implemented an in vitro assay that functionally resembles the DNA repair reaction and assays to study protein-protein and protein-DNA interactions. (uu.nl)
1 Using nano differential scanning calorimetry to make such measurements can also be used to provide general structure information and investigate binding events, particularly for protein-ligand interactions. (tainstruments.com)
The proposed project aims to identify the dynamics of RNA-protein interactions during DNA damage response (DDR) using high-throughput "omics" approaches. (europa.eu)
3) Protein interactions and enzyme characterization. (manchester.ac.uk)
Here, we present an interdisciplinary characterization of the interactions between Rad51 and these factors. (woofahs.com)
Comparison between the effect on the L3 phase of encapsulated beta-galactosidase (238 kDa), aspartic protease (34 kDa), myoglobin (17.6 kDa) and phytoglobin BvPgb 1.2 (38.4 kDa) demonstrated the importance of specific lipid-protein interactions on the L3 structure both in bulk and dispersions, as well as the stability of the encapsulated proteins. (lu.se)

When compared to similar techniques like isothermal titration calorimetry (ITC) it has reduced protein consumption. (utmb.edu)

Thus, our biophysical characterization is a first step towards structure determination of the BteA N-terminal domain. (nih.gov)

You have hands-on experience and trouble-shooting proficiency with analytical and characterization techniques for biomolecules, such as column chromatographic (HPLC/UPLC), capillary electrophoresis and/or biophysical techniques (such as DLS, CD, FTIR, DSC, AUC) for protein characterization. (biospace.com)
Encapsulation in self-assembled lipid structures has been shown to increase the stability of large biomolecules, including proteins and nucleic acids (NAs), which has many potential applications in the food and pharmaceutical industries. (lu.se)

Genomic characterization of SARS-CoV-2 in Egypt: insights into spike protein thermodynamic stability. (cdc.gov)

Concomitantly, substantial progress was made in pharmaceutical formulation development of peptides and proteins, inasmuch as many challenges in formulating these compounds in products with optimal therapeutic effects and shelf life were successfully approached. (uni-muenchen.de)

Emulsions prepared in this manner exhibited superior oxidative stability than those prepared by conventional methods utilizing homogenizers. (bvsalud.org)
Given the diversity of biological functions and biomedical applications borne by EV, there is an urgent need for efficient and reliable methods of EV characterization and isolation, to improve our understanding on EV and fully exploit their potential. (selectbiosciences.com)
Analysis of Host Cell Proteins (HCPs): Methods, Challenge. (pharmiweb.com)
To effectively assess and control the risks associated with HCPs in therapeutic protein products, it is crucial to develop methods that can identify and monitor all HCP components. (pharmiweb.com)
However, the major challenge with MS-based methods is the large concentration difference between HCPs (usually in low ppm levels) and the target protein (high concentration). (pharmiweb.com)
This book discusses flavor, texture, and characterization methods to improve the freshness or shelf-life of a product. (chipsbooks.com)
It was the aim of this thesis to evaluate the applicability of asymmetrical flow field flow fractionation (AF4) in pharmaceutical protein analytics, to compare AF4 performance with established state-of-the-art methods and to reveal the effectivity of inherent AF4 characteristics in demanding analytical tasks. (uni-muenchen.de)
Currently there exist methods to stabilize proteins with enzymatic functions or with certain characteristic reactions. (lu.se)

These limitations include a lack of available tools for biochemical characterization of enzymes involved in glycosylation. (biorxiv.org)
His research interest includes molecular characterization, structure-function relationship of enzymes that is useful for industrial purpose. (omicsonline.org)
The structure determination of this protein will be helpful to improve the stability of the enzymes by mutational studies and thus modulate the function of these important enzymes. (omicsonline.org)
Enzymes used in biotechnology have to have increased stability to function in harsh conditions. (lu.se)

Molecular characterization of these benign yet rapidly proliferating tumors has been limited to evaluating a few mutations in few genes. (frontiersin.org)
Systems that previously were considered too complex for a molecular characterization now successively become available to fundamental studies. (lu.se)

You have strong technical knowledge base and proven experience with biophysical/biochemical characterization, bioanalytical method development or biomolecule product development. (biospace.com)

TA Instruments Nano DSC is an ultrasensitive nano differential scanning calorimetry instrument for reliable measurements of antibody and other macromolecule stability. (tainstruments.com)
Designed to be easy to use and capable of performing measurements with as little as 2 µg of sample, the Nano DSC is the ideal nano differential scanning calorimetry instrument for monitoring antibody stability for routine and research purposes. (tainstruments.com)

Proteins are biological molecules that have an important application as a catalyst for various reactions. (uconn.edu)
Hsp90's diverse array of clientele implicate the chaperone in several associated biological functions and place it at the intersection of various fundamental cellular pathways, where it acts as a central hub in maintaining numerous protein interaction networks 1 . (nature.com)
Yet, due to the proneness of proteins to degradation - what can affect pharmaceutical relevant features such as biological activity and immunogenicity -, scrutinizing the homogeneity of protein formulations is of utmost importance. (uni-muenchen.de)
Proteins are involved in virtually all biological processes and their structure is key to their function. (manchester.ac.uk)
To provide core knowledge of the form, function and role of protein molecules in biological processes and further enable an understanding of biological phenomena at the molecular level. (manchester.ac.uk)
We have established protocols that allow for reliable liposome synthesis and for physical, chemical and biological characterization. (uni-regensburg.de)

Due to the AF4 feature to discretionarily alter the resolution power within one separation run, the fractionation of higherorder aggregates and insoluble, precipitated protein was successfully performed. (uni-muenchen.de)
Aura PTx is a complete protein therapeutic stability solution that enables characterization of subvisible particle aggregates from developability assessment through product release. (bioprocessintl.com)
Aura PTx is a high throughput analytical instrument that images, counts, sizes, and identifies subvisible particle aggregates, a critical quality attribute and the most important biologic stability parameter. (bioprocessintl.com)
They are intracellularly located as protein 'aggregates' within cellular protein vacuoles, and also within the cytoplasm. (indexindex.com)

Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication due to their ability to transfer bioactive lipids, proteins and different species of RNA into cells. (selectbiosciences.com)
Glucose is a direct energy source, and galactose may be used as a component of complex proteins or lipids (glycoproteins and glycolipids). (researchgate.net)
The ATP8B1 gene provides instructions for making a protein that helps to control the distribution of certain fats, called lipids, in the membranes of liver cells. (medlineplus.gov)
In the first part of my thesis, the encapsulation of different proteins in a lipid sponge phase (L3) system composed of food grade lipids was studied. (lu.se)

Differential scanning fluorimetry (DSF) that measures the intrinsic fluorescence from your protein (Tryptophans and Tyrosines), hence no reporter dye is needed. (lu.se)

abstract = "Self-assembling protein nanocontainers are promising candidates for an increasingly wide scope of purposes. (vu.nl)
Abstract Homologous recombination (HR) is essential for maintaining genome stability. (woofahs.com)

Select appropriate experimental techniques for protein purification and characterization (conformation and stability), and describe the relative precision of various approaches and the assumptions implied by each method. (manchester.ac.uk)

We have investigated liposomes for nucleic acid, protein, and whole cell detection, and their use for bioimaging and for multiplexing. (uni-regensburg.de)

The subunits associated into polymers depending on the protein concentration, pH value and ionic strength of the milieu and, therefore, a value for the MW of the native protein(s) is not given. (indexindex.com)
Allows a better characterisation of synthetic and natural polymers and proteins in solution. (lu.se)

In our present view, these areas are found for systems where several types of macromolecules (proteins, polysaccharides or polyelectrolytes) and/or amphiphiles interact to form a functional or destructive unit. (lu.se)

In this study, we describe the stability assessment of 5 mAbs and 6 associated coformulated mAb mixtures before and after thermal stress. (chi-peptalk.com)
The observed data suggest that the stabilities of antibody coformulations were found to be comparable to the stabilities of individual parental mAbs. (chi-peptalk.com)
THE fasted growing segment in the pharmaceutical industry is biologics (protein drugs), especially mAbs. (europeanpharmaceuticalreview.com)
A rigorous kinetic analysis not only provides T m s but, importantly, the denaturation rates and activation energies - two critical parameters for estimating the long-term stability of mAbs at lower temperatures. (europeanpharmaceuticalreview.com)

Solution molecular biophysics and thermodynamic techniques are used both as a primary research tool as well as means to structural studies (for example in the exploration of conditions for crystallization of proteins). (utmb.edu)
Thermodynamic data such as protein stability parameters and binding constants can also be obtained. (utmb.edu)
However, for a vast number of proteins there is no general method to improve their thermodynamic stability. (lu.se)

Emulsion stability was improved with increasing soy protein isolate (SPI) concentrations. (bvsalud.org)
At SPI concentrations higher than 20 mg/mL, the emulsions exhibited improved stability against changes in temperature , pH and salt concentration. (bvsalud.org)
Mass spectrometers often struggle to identify and detect low concentrations of HCPs amidst high concentrations of target proteins. (pharmiweb.com)
Concentrations and stability of methyl methacrylate, glutaraldehyde, formaldehyde and nickel sulfate in commercial patch test allergen preparations. (cdc.gov)

For some types of measurements, non-destructive approaches such as Raman spectroscopy may be appropriate, but this is not always suitable for evaluating antibody stability. (tainstruments.com)

A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. (biorxiv.org)
Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)
PRM analysis using high-resolution, high-precision mass spectrometry facilitates accurate identification and quantification of target proteins/peptides. (pharmiweb.com)

Parallel reaction monitoring (PRM) is a targeted proteomics technology that enables the selective detection of specific proteins and peptides for absolute quantification. (pharmiweb.com)

Anti-PnMas1 and anti-PnMas2 antibodies co-labelled two high-molecular-weight (~400 kDa) protein complexes in native gels but anti-PnMas3 antibodies labelled a 65 kDa protein complex. (springer.com)
Learn how ITC can be used to guide protein (RNA)/ligand crystallization and to resolve common issues with crystallization of complexes. (malvernpanalytical.com)

Substitution of Val 113 in Sendai virus (SeV) M protein generates non-functional polypeptides, characterized by their exclusion from virus particles and by their ability to interfere with virus particle production. (microbiologyresearch.org)
The authors concluded that the contribution of particular side chains to the conformational stability of FXN might be highly subordinated to their impact on both the protein function and the stability of the functional supercomplex. (curefa.org)
For example, proteins delivered as drugs must maintain their functional form at elevated temperatures and when formulated in drug delivery systems. (lu.se)

The BCC offers biomolecular crystallisation and characterisation services to CSIRO clients. (www.csiro.au)
The Biomolecular Crystallisation and Characterisation Centre (BCC), is Australia's only full-service crystallisation centre. (www.csiro.au)
Please visit our Biomolecular Crystallisation and Characterisation website for full details of how to use the service. (www.csiro.au)

The storage proteins are not glycoproteins. (indexindex.com)

Our Surface Plasmon Resonance (SPR) Biacore T100 biosensor instrument is a high-throughput equipment that allows the study of protein-protein and protein-ligand kinetics and thermodynamics. (utmb.edu)

The Jasco J-815 Circular Dichroism Spectrometer housed in the SBL allows for the quick and easy determination of secondary and tertiary structures of protein samples. (utmb.edu)
You will learn how proteins are produced, how they fold into their secondary and tertiary structures and how they interact with each other. (manchester.ac.uk)

Aggregation of VSV M protein is reversible and mediated by nucleation sites: implications for viral assembly. (microbiologyresearch.org)
The Theoretical Section encompasses Chapter 2, wherein the family of field-flow fractionation techniques is introduced, as well as Chapter 3 (attending to protein aggregation) and Chapter 4, which provides an insight into multi-angle light scattering. (uni-muenchen.de)
The larger the activation energy, the faster the decrease in rate of denaturation/aggregation at lower temperatures and consequently the better long-term stability. (europeanpharmaceuticalreview.com)
Used to study the aggregation state of proteins. (lu.se)

Blackman LM, Arikawa M, Yamada S, Suzaki T, Hardham AR (2011) Identification of a mastigoneme protein from Phytophthora nicotianae . (springer.com)
The data generated can be used by pharmaceutical clients to support their regulatory submissions and chemistry, manufacturing and controls (CMC) activities including product characterisation, stability, commercial release testing and identification and quantification of low level impurities and process residuals. (intertek.com)
The latter allows the identification of even very small crystals hidden in precipitate, or discrimination of protein crystals from salt crystals. (lu.se)

BteA, a 69-kDa cytotoxic protein, is a type III secretion system (T3SS) effector in the classical Bordetella, the etiological agents of pertussis and related mammalian respiratory diseases. (nih.gov)

Allows setting up crystallisation drops with volumes as low as 50 nl protein + 50 nl reservoir and 1-3 protein samples per condition, thus 96-288 experiments can be done in one plate. (lu.se)
The effect of buffer conditions and additives on protein stability can be probed, which is useful in preparation for a crystallisation experiment. (lu.se)

The MWCO-based HCP characterization technique involves the following steps: first, the sample is treated with surfactant to release the interaction between the target protein and HCPs. (pharmiweb.com)
Yet, the retaining impact of sample-membrane interaction was evidenced to decrease the effective diffusion coefficient, resulting in successful fractionations of proteins differing ~1% in size (i.e. (uni-muenchen.de)
Distinguish between various modes of protein-protein interaction and catalysis. (manchester.ac.uk)

The transient receptor potential melastatin 4 (TRPM4) protein is an intracellular Ca 2+ -activated non-selective cation channel, which is impermeable to Ca 2+ . (frontiersin.org)
Methylenediphenyl diisocyanate binds membrane and intracellular proteins of intact THP-1 cells. (cdc.gov)

Antibody stability is crucial for antibody performance in therapeutics. (tainstruments.com)
Another alternative is nano differential scanning calorimetery (DSC), which is one of the best ways of measuring antibody stability with minimal sample preparation and consumption. (tainstruments.com)
How can I measure antibody stability easily and with lower sample consumption? (tainstruments.com)
What is Nano DSC, and how is it used to measure antibody stability? (tainstruments.com)
This nano differential scanning calorimeter can be used to measure with great sensitivity antibody stability by performing temperature ramps on very small amounts of sample. (tainstruments.com)
Why is measuring antibody stability important? (tainstruments.com)
Measuring antibody stability during the research and development phase of new therapeutic antibodies is very important as the stability is often one of the design parameters that needs to be considered as part of the research process. (tainstruments.com)
Why is Nano DSC the best option for measuring antibody stability? (tainstruments.com)
Data can be imported directly into the NanoAnalyze software for model fitting and multi-batch file processing to accelerate the extraction of important parameters for your antibody stability analysis. (tainstruments.com)
Contact TA Instruments today for an expert opinion on how nano differential scanning calorimetery will simplify your antibody stability analysis. (tainstruments.com)

The growth of the protein stability analysis market is primarily driven by the growing adoption of open innovation models in pharmaceutical & biotechnology companies and increasing pharmaceutical outsourcing. (marketsandmarkets.com)
The extensive use of protein stability analysis in pharmaceutical & biotechnology companies for drug discovery applications will drive the market during the forecast period. (marketsandmarkets.com)
By end user, the protein stability analysis market is segmented into pharmaceutical and biotechnology companies, contract research organization, and academic & research institutes. (marketsandmarkets.com)
The large share of this segment is primarily due to the extensive use of protein stability analysis in pharmaceutical & biotechnology companies for drug discovery applications. (marketsandmarkets.com)
A fundamental problem in protein science, biomedicine, biotechnology and the pharmaceutical industry is to develop proteins with increased stability. (lu.se)

The 90 KDa heat shock protein (Hsp90) is a highly conserved molecular chaperone crucially involved in maintaining cellular homoeostasis in organisms from most kingdoms of life with the exception of archea 1 . (nature.com)
but molecular and cellular characterization has been limited. (frontiersin.org)
Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
The major proteins of the yam tuber, which were identified as storage proteins by virtue of their abundance (ca 85 % of the total protein content), amino acid composition (high in amide content) and cellular location within the tuber, were isolated by ion-exchange chromatography and characterized using, in particular, polyacrylamide gel techniques. (indexindex.com)

Moreover, in contrast to SE-HPLC, AF4 was capable of seizing undissolved and precipitated protein specimen, thus making AF4 a promising alternative in the analysis of protein pharmaceuticals. (uni-muenchen.de)
It is well documented that physio-chemical activity as well as long-term stability of protein-based pharmaceuticals are directly influenced by ice crystal formation and their contribution to surface area. (pharmaceutical-technology.com)

By using the NanoAnalyzer, single-particle characterization can be achieved which simultaneously measures the side scatter (40 -1000nm) and fluorescent properties of particles. (selectbiosciences.com)
For example, their mechanical properties give insight into the stability of a particle as a function of their internal content. (vu.nl)

The work supports a role for chemical modification in the recognition and subsequent degradation of a key protein subunit of PSII by a bacterial-type protease, suggesting that tryptophan oxidation of components of the photosynthetic apparatus after high light stress plays a critical role in initiating the PSII repair system. (elifesciences.org)

CDV-enriched proteins were found by proteomic analysis and confirmed by nFCM. (selectbiosciences.com)
Proteomic data analysis involves searching against the UniprotKB murine protein database, considering various modifications, cleavage deletions, and false discovery rate settings. (pharmiweb.com)

Due to enormous progress in recombinant DNA techniques and methodology, a multitude of biosynthetic, pharmaceutically relevant polypeptides and proteins became available in the past decade and have been employed in numerous pharmaceutical products. (uni-muenchen.de)

On the basis of products, the protein stability analysis market is divided into reagents & assay kits, instruments, consumables & accessories, and software. (marketsandmarkets.com)
The large share of this market segment can be attributed to the high consumption of reagents and assay kits in protein stability analysis techniques, increasing protein pharmaceutical R&D, and increased government funding for life science research. (marketsandmarkets.com)
The most commonly used method for HCP assay in therapeutic protein production is the enzyme-linked immunosorbent assay (ELISA). (pharmiweb.com)

In the current study, genes encoding two related proteins, PnMas1 and PnMas3, were identified in the genome of P. parasitica . (springer.com)
These irregular structures must be corrected to maintain genome stability, integrity and fidelity. (uu.nl)

Intrinsically disordered proteins and their contraction and folding can be monitored using this technique. (utmb.edu)
This research report categorizes the protein stability analysis market based on Product, Technique, End User, and Region. (marketsandmarkets.com)
In the MWCO-based technique, target protein samples containing HCPs are dried and dissolved in Tris HCl dissociation buffer containing surfactants. (pharmiweb.com)
The MWCO-based technique, combined with Shotgun proteomics analysis, offers a simple and efficient approach to identify HCPs in therapeutic protein drug products. (pharmiweb.com)
The emphasis of the course is on practical applications, for example selecting the best technique to address a particular question on protein structure and function. (manchester.ac.uk)
Used for studying the thermal unfolding of proteins using the Thermofluor technique with a dye as a reporter (differential scanning fluorimetry, DSF). (lu.se)

Quantitative proteomics will be applied to identify the differentially mRNA-bound proteins after the exposure of human cultured cells to ionizing radiation. (europa.eu)

Whereas up to now cross flow intensity was deemed to predominantly account for contingent sample loss during AF4 experiments, analysis of proteins with varying hydrophilicity proved the preceding focusing step to contribute notably for that phenomenon. (uni-muenchen.de)

The sensitivity of protein to denature at high temperature limits its industrial application where a catalyst that works at high temperature is required. (uconn.edu)

Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. (biorxiv.org)

We probe the open conformation of human Hsp90α for druggable sites that overlap with these allosteric control elements, and identify three putative natural compound allosteric modulators: Cephalostatin 17, 20(29)-Lupene-3β-isoferulate and 3′-Bromorubrolide F. We assess the allosteric potential of these ligands by examining their effect on the conformational dynamics of the protein. (nature.com)
This will provide a global insight into the dynamics of RNAs and proteins during DDR. (europa.eu)

The BCC is designed around facilitating access to crystallization and characterisation services for the clients that do not have access to either the equipment or expertise required for their scientific research campaign. (www.csiro.au)

In this study, the authors prepared a set of FXN variants spanning a broad range of conformational stabilities. (curefa.org)

UTMB also pioneered the use of osmolytes in the stabilization of intrinsically unstructured regions of proteins ( 2 ) and the SBL has a long tradition of the use of protecting osmolytes in biophysical studies ( 3 ). (utmb.edu)

Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. (biorxiv.org)

Parameter control, characterization and stability of soy protein emulsion prepared by microfluidic technology. (bvsalud.org)

While single molecule manipulation techniques now routinely provide access to these, there is a need to move towards predictive tools that can rationally identify proteins with desired material properties. (whiterose.ac.uk)

Clients involved in biopharmaceutical product development will benefit from regulatory NMR data supporting protein characterisation. (intertek.com)
E7 from human papil omavirus type 16 cooperate to cancer: the European prospective investigation into doi:10.1093/carcin/bgp321 PMID:20047954 target the PDZ protein Na/H exchange regulatory cancer and nutrition study. (who.int)

Although the secondary structure was protected for thermal denaturation the activity was lost for most of the proteins studied and SDS-PAGE gel showed that covalent modification of protein with capric acid resulted in high temperature heating. (uconn.edu)
Describe the features of proteins which lead to successful folding, post-translational modification and degradation. (manchester.ac.uk)

The specifi c projects in the area that are currently pursued at the department can be classifi ed into four general themes: i) Colloidal stability and cooperative association. (lu.se)
Using a combination of small angle x-ray and neutron scattering (SAX/NS), dynamic light scattering (DLS) and cryoTEM, the colloidal stability, LNP morphology, lipid composition of the LNP core-shell structure and structure of the core were determined. (lu.se)

Anatomy12

Organisms8

Diseases1

Chemicals and Drugs106

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Phenomena and Processes87

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science7

Health Care3

Product characterization2

Forced degradation1

Monoclonal antibodies6

Interactions8

Isothermal Titration C1

Biophysical characterization1

Biomolecules2

Genomic1

Formulation development1

Methods8

Enzymes4

Molecular characterization2

Biochemical characterization1

Differential Scanning Ca2

Biological6

Aggregates4

Lipids4

Fluorescence1

ABSTRACT2

Purification1

Nucleic acid1

Polymers2

Macromolecules1

MAbs4

Thermodynamic3

Concentrations4

Spectroscopy1

Target proteins3

Peptides1

Complexes2

Functional3

Biomolecular3

Glycoproteins1

Kinetics1

Secondary and tertiary structures2

Aggregation4

Identification3

Effector1

Crystallisation2

Interaction3

Intracellular2

Antibody10

Biotechnology5

Cellular4

Pharmaceuticals2

Particle2

Subunit1

Proteomic2

Recombinant1

Assay3

Genome2

Technique6

Proteomics1

Experiments1

Sensitivity1

Glycosylation1

Dynamics2

Crystallization1

Conformational1

Intrinsically1

Enables1

Parameter1

Manipulation1

Regulatory2

Modification2

Colloidal2