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cdc42 GTP-Binding Proteincdc25 PhosphatasesCDC2 Protein KinaseCdc20 ProteinsCell Cycle ProteinsProtein-Serine-Threonine Kinasescdc42 GTP-Binding Protein, Saccharomyces cerevisiaeProtein KinasesPhosphatidylinositol 3-KinasesMAP Kinase Signaling SystemSaccharomyces cerevisiae ProteinsUbiquitin-Protein Ligase ComplexesF-Box ProteinsAnaphase-Promoting Complex-CyclosomeCDC2-CDC28 KinasesCalcium-Calmodulin-Dependent Protein KinasesSchizosaccharomyces pombe ProteinsProtein Kinase InhibitorsFungal ProteinsCyclin BPhosphorylationSchizosaccharomycesMitosissrc-Family KinasesSaccharomyces cerevisiaeCDC28 Protein Kinase, S cerevisiaeTelomere-Binding ProteinsCell CycleProtein BindingMolecular Sequence DataMutationCyclin-Dependent Kinase Inhibitor Proteinsp21-Activated KinasesAmino Acid SequenceProtein Kinase Cp38 Mitogen-Activated Protein KinasesCyclic AMP-Dependent Protein KinasesCyclin-Dependent KinasesSignal TransductionMitogen-Activated Protein Kinase 1Ubiquitin-Protein LigasesProtein Structure, TertiaryRecombinant Fusion ProteinsJNK Mitogen-Activated Protein KinasesProtein-Tyrosine KinasesMitogen-Activated Protein Kinase KinasesGTP-Binding ProteinsCytokinesisMitogen-Activated Protein Kinase 3MAP Kinase Kinase KinasesSequence Homology, Amino AcidLigasesCreatine KinaseHeLa CellsGenes, cdcCasein Kinase IINuclear ProteinsCasein KinaseseIF-2 KinaseRibosomal Protein S6 KinasesPyruvate KinaseGuanine Nucleotide Exchange FactorsMAP Kinase Kinase 1Models, BiologicalDNA ReplicationReceptor Protein-Tyrosine KinasesMAP Kinase Kinase 4Mitogen-Activated Protein KinasesThymidine KinaseTelomereExtracellular Signal-Regulated MAP KinasesEnzyme ActivationPhosphotransferases (Alcohol Group Acceptor)1-Phosphatidylinositol 4-KinaseAurora Kinasesrho-Associated KinasesProto-Oncogene ProteinsGlycogen Synthase Kinase 3Base SequenceI-kappa B KinaseIsoenzymesTransfectionCyclin-Dependent Kinase 2KineticsProtein Kinase C-alphaBlotting, WesternProtein Kinase C-deltaCell LineIntracellular Signaling Peptides and ProteinsPhosphoproteinsSubstrate SpecificityDiacylglycerol KinaseRecombinant ProteinsTyrosineAMP-Activated Protein KinasesBinding SitesProto-Oncogene Proteins c-aktG2 PhaseSerinePhosphoprotein Phosphatases
PhosphorylationCyclinsMitoticCyclin-dependent protein kinasesP34CDC2PhosphorylatesCDK1MitosisSpecificitySubstrateCerevisiaeSerineInhibitorCDNASubunitGenesCDK4High-throughputGeneEmbryoRegulatoryMolecularCdc6AntibodyNuclearPromotesFunctionalDetectsPhaseActivitySubstratesInteractionRolesAbsenceOccurComplexesComplexInteractionsMajor
Phosphorylation9
  • This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). (thermofisher.com)
  • v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity. (ox.ac.uk)
  • In particular, hormonal activation of a conditional v-Jun-estrogen receptor fusion protein in quiescent, growth factor-deprived cells stimulates cyclin E-cdk2 activity and triggers Rb phosphorylation and DNA synthesis. (ox.ac.uk)
  • instead of attempting to predict individual phosphorylation sites, we search for proteins that contain high densities of strong and weak consensus matches that are closely spaced in the primary amino acid sequence. (biomedcentral.com)
  • The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. (wikidata.org)
  • mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2. (wikidata.org)
  • Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. (nature.com)
  • Although we did not identify any highly Clb2-specific substrates, we found that Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets. (nature.com)
  • Cyclin-dependent kinases are regulated by phosphorylation and dephosphorylation events. (jefferson.edu)
Cyclins7
  • The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). (thermofisher.com)
  • Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. (thermofisher.com)
  • A relatively well understood example is the network that controls progression of the cell cycle, where cyclin-dependent kinases (CDKs) couple with various cyclins over the cell cycle to regulate critical processes [ 2 - 4 ]. (biomedcentral.com)
  • Cell-cycle events are controlled by cyclin-dependent kinases (CDKs), whose periodic activation is driven by cyclins. (nature.com)
  • Fisher, D. L. & Nurse, P. A single fission yeast mitotic cyclin B p34 cdc2 kinase promotes both S-phase and mitosis in the absence of G1 cyclins. (nature.com)
  • It is widely assumed that degradation of mitotic cyclins causes a decrease in mitotic cdc2/CDC28 kinase activity and thereby triggers the metaphase to anaphase transition. (ox.ac.uk)
  • Protein kinases that control cell cycle progression in all eukaryotes and require physical association with CYCLINS to achieve full enzymatic activity. (jefferson.edu)
Mitotic4
  • The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis. (wikidata.org)
  • Destruction of the CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast. (ox.ac.uk)
  • Two observations made on the budding yeast Saccharomyces cerevisiae are inconsistent with this scenario: (i) anaphase occurs in the presence of high levels of kinase in cdc15 mutants and (ii) overproduction of a B-type mitotic cyclin causes arrest not in metaphase as previously reported but in telophase. (ox.ac.uk)
  • Recent breakthroughs have uncovered more and more DNA replication licensing machinery proteins (ORC, Cdc6, Cdt1, geminin, etc.) functioning in other cell cycle events, including centrosome replication, mitotic events, transcription and so on. (intechopen.com)
Cyclin-dependent protein kinases2
  • Differential expression of genes for cyclin-dependent protein kinases in rice plants , 1999, Plant Physiol. (github.io)
  • A rice homolog of Cdk7/MO15 phosphorylates both cyclin-dependent protein kinases and the carboxy-terminal domain of RNA polymerase II , 1998, The Plant Journal. (github.io)
P34CDC21
Phosphorylates2
  • In the cell nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. (wikipedia.org)
  • p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II. (prosci-inc.com)
CDK12
  • About 24% of these proteins were phosphorylated more efficiently by Clb5-Cdk1 than Clb2-Cdk1. (nature.com)
  • Targets of the cyclin-dependent kinase Cdk1. (nature.com)
Mitosis2
  • Stern, B. & Nurse, P. A quantitative model for the cdc2 control of S phase and mitosis in fission yeast. (nature.com)
  • Kinase destruction is therefore implicated in the exit from mitosis rather than the entry into anaphase. (ox.ac.uk)
Specificity4
  • Dual specificity protein kinase CLK1 is an enzyme that in humans is encoded by the CLK1 gene. (wikipedia.org)
  • This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. (wikipedia.org)
  • Because of the low specificity of the CDK consensus, however, databases of protein sequences are expected to contain large numbers of matches by chance. (biomedcentral.com)
  • Brown, N. R., Noble, M. E., Endicott, J. A. & Johnson, L. N. The structural basis for specificity of substrate and recruitment peptides for cyclin-dependent kinases. (nature.com)
Substrate2
Cerevisiae3
  • This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. (thermofisher.com)
  • We show that it is possible to predict proteins that are likely to be targets of CDKs in S. cerevisiae by searching for proteins that contain clustered matches to the CDK consensus. (biomedcentral.com)
  • We also show that human CDK targets are enriched for proteins that contain clustered consensus matches and, by searching human cell cycle genes, we predict several putative CDK targets, including the human orthologs of Schizosaccharomyces pombe CDC5 (CDC5L) and S. cerevisiae Cdc20p (CDC20). (biomedcentral.com)
Serine2
  • The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine-rich proteins. (wikipedia.org)
  • To examine the involvement of interchromatin granule clusters (IGCs) in transcription and pre-mRNA splicing in mammalian cell nuclei, the serine-arginine (SR) protein kinase cdc2-like kinase (Clk)/STY was used as a tool to manipulate IGC integrity in vivo. (rupress.org)
Inhibitor1
  • Moreover, mH2A1.1-OE proteomic profile identified several upregulated proteins associated to DNA and histones hypermethylation, including S-adenosylhomocysteine hydrolase, a strong inhibitor of DNA methyltransferase and of the methyl donor S-adenosyl-methionine (SAM). (bvsalud.org)
CDNA2
  • Characterization by cDNA cloning of two new human protein kinases. (wikipedia.org)
  • Isolation and characterization of cDNA clones encoding cdc2 homologues from Oryza sativa: a functional homologue and cognate variants , 1992, Mol Gen Genet. (github.io)
Subunit1
Genes2
  • We use basic and clinical research to understand the cells, proteins, and genes that lead to successful recovery of function, and also how complications develop that impact quality of life after stroke. (stanford.edu)
  • In 7 MM, 3 MGUS and 3 healthy subjects by gene expression profile, we identified a total of 551 upregulated and 343 downregulated genes in MM-HDN, involved in chemokine signaling pathway and FC-gamma receptor mediated phagocytosis conveying in the activation of STAT proteins. (bvsalud.org)
CDK42
  • CDK4 is a member of the Ser/Thr protein kinase family. (thermofisher.com)
  • Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. (ayassbioscience.com)
High-throughput1
  • Over the past decade, yeast two-hybrid, mass spectrometry and other high-throughput experimental have generated a mass of protein-protein interaction (PPI) data. (biomedcentral.com)
Gene2
  • The protein encoded by this gene is a member of the Ser/Thr protein kinase family. (technuc.com)
  • Protein kinases are critical to cellular signalling and post-translational gene regulation, but their biological substrates are difficult to identify. (biomedcentral.com)
Embryo1
  • The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes. (wikidata.org)
Regulatory1
  • Our data suggest that regulatory modules may exist in protein sequence as clusters of short sequence motifs. (biomedcentral.com)
Molecular2
  • A protein complex consists of a group of proteins and multiple PPIs at the same time and place, forming single multi-molecular machinery [ 4 ]. (biomedcentral.com)
  • It is necessary to construct dynamic PPI networks for both identifing protein complexes and further understanding molecular systems. (biomedcentral.com)
Cdc61
  • The Clb5-specific targets include several proteins (Sld2, Cdc6, Orc6, Mcm3 and Cdh1) involved in early S-phase events. (nature.com)
Antibody2
  • CDC2 (Ab-161) antibody was raised against a peptide sequence around aa.159~163 (T-Y-T-H-E) derived from Human CDC2. (prosci-inc.com)
  • This antibody detects endogenous level of total CDC2 protein. (prosci-inc.com)
Nuclear1
  • Conversely, overexpression of a catalytically inactive mutant, Clk/STY(K190R), causes retention of hypophosphorylated SR proteins in nuclear speckles. (rupress.org)
Promotes1
  • Schindler K, Benjamin KR, Martin A, Boglioli A, Herskowitz I, Winter E. The Cdk-activating kinase Cak1p promotes meiotic S phase through Ime2p. (jefferson.edu)
Functional1
  • Since most proteins are only functional after assembly into protein complexes, protein complexes are critical in many biological processes [ 5 ]. (biomedcentral.com)
Detects1
  • 11 ] present COACH algorithm to identify protein complexes, which detects the core structure and attachments of complex respectively. (biomedcentral.com)
Phase1
  • We show that v-Jun enables cells to express cyclin A and cyclin A-cdk2 kinase activity in the absence of growth factors and that deregulation of cdk2 is required for S-phase entry. (ox.ac.uk)
Activity1
  • The execution of anaphase and the destruction of CDC28 kinase activity therefore appear to take place independently of one another. (ox.ac.uk)
Substrates1
  • Identification of a cyclin-cdk2 recognition motif present in substrates and p21-like cyclin-dependent kinase inhibitors. (nature.com)
Interaction1
  • Recently, researchers have tried to integrate various dynamic information with static protein-protein interaction (PPI) networks to construct dynamic PPI networks. (biomedcentral.com)
Roles1
  • A multifunctional CDC2 kinase-related kinase that plays roles in transcriptional elongation, CELL DIFFERENTIATION , and APOPTOSIS . (nih.gov)
Absence1
  • The behaviour of esp1 mutants shows in addition that kinase destruction can occur in the absence of anaphase completion. (ox.ac.uk)
Occur1
  • We also find that these clusters tend to occur in disordered or unfolded regions near the termini of the protein. (biomedcentral.com)
Complexes1
  • Then, we use the uncertain model to identify dynamic protein complexes in the dynamic uncertain PPI networks. (biomedcentral.com)
Complex3
  • The experimental results show that our approach can effectively deal with the uncertain data in dynamic uncertain PPI networks, and improve the performance for protein complex identification. (biomedcentral.com)
  • 10 ] propose CORE algorithm to identify protein-complex cores by calculating the p -values for all pairs of proteins. (biomedcentral.com)
  • A protein complex is formed by a group of proteins at the same time, which interacted with each other by associated polypeptide chains. (biomedcentral.com)
Interactions1
  • Our data suggest that the protein-protein interactions responsible for the clustering of interchromatin granules are disrupted when SR proteins are hyperphosphorylated and stabilized when SR proteins are hypophosphorylated. (rupress.org)
Major1
  • This graph shows the total number of publications written about "Cyclin-Dependent Kinases" by people in this website by year, and whether "Cyclin-Dependent Kinases" was a major or minor topic of these publications. (jefferson.edu)