Protein cd63. Medical search. Frequent questions

SDS-PAGE Analysis Purified CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5). (neobiotechnologies.com)
Analysis of Protein Array containing more than 19,000 full-length human proteins using CD63-Monospecific Mouse Recombinant Monoclonal Antibody (rMX-49.129.5) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. (neobiotechnologies.com)
Immobilized human CLDN18.2-VLP protein at 0.5μg/per well binds to Anti-CLDN18.2 monoclonal antibody. (dimabio.com)
Immobilized human CD63-synthetic nanodisc protein at 0.2μg/per well binds to Anti-CD63 monoclonal antibody (DMC100425). (dimabio.com)

CD63 had functions in mediating signal transduction processes and then regulate variety of cellular processes such as cell proliferation, activation and motility. (abeomics.com)
The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. (networkin.info)
Abundant proteins, including membrane trafficking molecules, cytoskeleton molecules, chaperones, signal transduction proteins and cytoplasmic enzymes have been included in secreted exosomes [ 9 ]. (oncotarget.com)

Cluster of differentiation 63 (CD63) is a member of the CD family and the transmembrane 4 superfamily,also known as the tetraspanin family. (abeomics.com)
The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. (neobiotechnologies.com)
Human mast cell secreted exosomes carrying the tetraspanin membrane protein CD63 were analyzed by measuring their diffusion-limited binding rate to an SPR sensor surface functionalized with anti-CD63 antibodies. (fu-berlin.de)
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. (networkin.info)

Exosomes are 40-100 nm soluble microvesicles with a bi-lipid membrane and a cargo abundant in proteins and RNAs, which are now believed to be important for intercellular communication 9 . (nature.com)
These EV contain a lipid bilayer associated to membrane proteins. (frontiersin.org)
The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. (neobiotechnologies.com)
For single pass membrane proteins, DIMA offers Extracellular Domain (ECD) proteins that demonstrate exceptional activities and stabilities. (dimabio.com)
Full length membrane proteins are often unstable and rapidly lose activity after solubilization in buffer. (dimabio.com)
DIMA Biotech has pooled expertise in the cutting-edge technologies of membrane proteins and developed several development platforms for different proteins. (dimabio.com)
Rab GTPases, as intracellular transport proteins, are master regulators of exocytic and endocytic membrane trafficking [ 10 ]. (oncotarget.com)

CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). (biolegend.com)

Munc13-4 is an essential protein in the intracellular trafficking and exocytosis of lytic granules. (wikipedia.org)
CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. (neobiotechnologies.com)
In the tumor microenvironment, cell migration and invasion are regulated by many factors such as intracellular signaling pathways, growth factors or onco-proteins [ 2 ]. (oncotarget.com)

Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication due to their ability to transfer bioactive lipids, proteins and different species of RNA into cells. (selectbiosciences.com)
Structure, function and organization of lipids and proteins in biological membranes. (biomembranes.nl)
Biogenesis of membranes and the transport and sorting of lipids and proteins. (biomembranes.nl)

Thrombin-activated human peripheral blood platelets were stained with CD63 (clone H5C6) Alexa Fluor® 488 (filled histogram) or mouse IgG1, κ Alexa Fluor® 488 isotype control (open histogram). (biolegend.com)

It has reported that CD63 protein associated with tumor progression and served as a blood platlet activation marker and the deficiency of this protein may be associated with Hermansky-Pudlak syndrome. (abeomics.com)
CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression. (neobiotechnologies.com)

Quality control test: Antibody Reactive Against Recombinant Protein. (novusbio.com)
ZEB1 (NP_110378, 801 a.a. ~ 900 a.a) partial recombinant protein with GST tag. (novusbio.com)
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. (novusbio.com)

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) (BME100001) in a linear range of 0.061-1.606 ng/ml. (dimabio.com)

CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. (biolegend.com)
CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. (neobiotechnologies.com)
Patients with Hermansky-Pudlak syndrome have been reported to lack the CD63 marker on platelets. (medscape.com)

CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6. (biolegend.com)
and transmembrane proteins CD63, CD9, and CD81. (elsevierpure.com)

Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)

CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. (biolegend.com)

The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion. (wikipedia.org)
As a secretory small GTPase, Rab3D is a vital regulator for protein secretion. (oncotarget.com)

For example, exosomes and nanovesicles secreted by living cells serve as intercellular couriers of mRNA, microRNAs (miRNAs), long noncoding RNAs (lncRNAs), and proteins and have thus garnered considerable interest [ 3 ]. (jcancer.org)

Lymphocyte migration regulation related proteins were differentially expressed in the urine exosome of lung cancer patients, and WASL, STK10 and WNK1 may serve as potential biomarkers for lung cancer diagnosis. (biomedcentral.com)
AIM OF THE STUDY: This study aims to analyze differentially expressed proteins (DEPs) in the plasma exosomes of patients with temporal lobe epilepsy (TLE) and after the Chaihu-Longgu-Muli Decoction (CLMD) therapy and to explore the biomarkers of TLE and the potential targets of CLMD in treating TLE. (bvsalud.org)

For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. (neobiotechnologies.com)
It also binds tetraspanins such as CD9, CD63 and CD151. (bio-techne.com)

Furthermore, pumilio homolog 2 (PUM2), a RNA-binding protein, mediated the packaging of miRNA-130a into exosomes. (dovepress.com)
But changes in the expression of lymphocyte migration regulation related proteins in urine exosomes remain unclear. (biomedcentral.com)
This study is to investigate the expression changes of lymphocyte migration regulation related proteins in urine exosomes of lung cancer patients, and further verify their correlation with the development and progression of lung cancer. (biomedcentral.com)
Lung cancer patients had reduced expression of WASL and increased expression of STK10 and WNK1 proteins in urine exosomes compared to normal people. (biomedcentral.com)
The proposed quantification method is based on a calibration and control measurements performed with proteins and synthetic lipid vesicles and takes into account (i) the influence of the broad size distribution of the exosomes on the surface coverage, (ii) the fact that their size is comparable to the ∼150 nm probing depth of SPR, and (iii) possible deformation of exosomes upon adsorption. (fu-berlin.de)
However, discriminating exosomes from microvesicles is difficult based on their physical properties or protein composition. (biomedcentral.com)
Among them, RPL6, NCL, and LDHA protein levels were downregulated and APOA1 protein level was upregulated in the plasma exosomes of TLE patients. (bvsalud.org)

Figure A. A647 conjugated mouse anti rat CD63 ( MCA4754A647 ) and FITC conjugated mouse IgG1 isotype control ( MCA1209F ). (bio-rad-antibodies.com)
Figure B. A647 conjugated mouse anti rat CD63 ( MCA4754A647 ) and FITC conjugated mouse anti rat CD61 ( MCA1773F ). (bio-rad-antibodies.com)

Evidence indicates that Alix, an accessory protein of the endosomal sorting complex required for transport (ESCRT), is involved in the biogenesis of extracellular vesicles (EVs). (spandidos-publications.com)
EVs released from HLSCs were enriched with miRNAs and expressed Alix and several RNA-binding proteins, including Argonaute 2 (Ago2), a member of the Argonaute family known to be involved in the transport and the processing of miRNAs. (spandidos-publications.com)

CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. (neobiotechnologies.com)

Analyzing the data of urine exosome proteomics, a total of 12 lymphocyte related proteins were identified, 5 of which were lymphocyte migration regulation related proteins. (biomedcentral.com)

The recombinant mouse CD63 consists 361 amino acids with a predicted molecular mass of 39.9 kDa. (abeomics.com)

CD63 is a cellular surface glycoprotein characterized by the presence of four bydrophobic domains. (abeomics.com)
The encoded protein is a cell surface glycoprotein that is known to complex with integrins. (networkin.info)

Exosome contain proteins, miRNAs, and mRNAs, and the exosomal lipid bilayer protects this genetic information from degradation. (biomedcentral.com)

A DNA sequence encoding the mouse Cd63 (NP_031679.1) (Ala103-Ile203) was expressed with the Fc region of human IgG1 at the N-terminus. (abeomics.com)

Next, they found that TIMP-1 from fibroblasts causes increased tumour proliferation and invasion when it interacts with the CD63 receptor on cancer cells. (ub.edu)
Receptor-ligand interaction A: Human SIRPα, hFc-His tagged protein without freeze-thaw treatment. (dimabio.com)

Biological activity of ABD-GCSF protein was assessed by proliferation assay on NFS-60 cells. (sciencegate.app)

Protein unc-13 homolog D, also known as munc13-4, is a protein that in humans is encoded by the UNC13D gene. (wikipedia.org)

Specifically, LAMP-1 and CD63 were identified as CDV-enriched markers and further confirmed by western blot analysis and flow cytometry (FACS). (selectbiosciences.com)

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. (neobiotechnologies.com)

DIMA Biotech offers a wide range of protein products made from mammalian cell expression systems. (dimabio.com)

Beyond the P protein, the Henipavirus P gene also encodes the V and W proteins which share with P their N-terminal, intrinsically disordered domain (NTD) and possess a unique C-terminal domain. (sciencegate.app)

Mouse anti Rat CD63 antibody 6H1 ( MCA4754GA ) used to demonstrate CD63 expressing cells by immunofluorescence. (bio-rad-antibodies.com)

based in the Barcelona Science Park, and professor at the University of Barcelona (UB), in collaboration with the Hospital Clínic has determined that the TIMP-1 protein is involved in the progression of lung adenocarcinoma. (ub.edu)

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind its native ligand Human SIRPα, hFc-His tagged protein (PME100009) in a linear range of 3.3-26.37 ng/ml. (dimabio.com)
ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PME100497) can bind Human ACE2, hFc Tagged protein (Stored at -20℃ or 37℃ for 24h, 48h and 72h) in a linear range of 0.488-49.83 ng/ml. (dimabio.com)

Immunohistochemical staining and western blot analysis were utilized to detect the protein expression of Foxp3、CD4、MBP、Notch1 and Hess1 in brain tissue. (researchsquare.com)
The protein level of key genes was detected using Western blot. (bvsalud.org)
Neuronal injury in the hippocampal dentate gyrus and ribosomal protein L6 (RPL6) expression in the brain tissues were detected using H&E staining, Nissl staining, and Western blot. (bvsalud.org)

Notably, a partial overlap was observed between CD63 and LAMP-1 positive EV subpopulations , suggesting distinct molecular properties and, perhaps, functional roles. (selectbiosciences.com)

APJ‑suppressed fibroblasts treated with an antagonist or small interfering RNA showed myofibroblast‑like properties, including increased proliferation and migratory abilities, via accelerated phosphorylation of Sma‑ and Mad‑related protein 2/3 (Smad2/3). (spandidos-publications.com)
TIMP-1 is a protein unusually secreted by fibroblasts, a type of non-malignant cell that accompanies cancer cells and is the main component of what is called the tumour microenvironment. (ub.edu)
The results first showed that elevated levels of TIMP-1 in lung cancer are due to fibroblasts secreting "pathological levels" of this protein. (ub.edu)

Structural and molecular information on Henipavirus W proteins is lacking. (sciencegate.app)

Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)

a Immunofluorescence of CD63 (green) and mTOR (red) in INS1 cells treated with control (1% BSA, 11 mM glucose, "1% BSA") or glucolipotoxic (1% BSA, 33.3 mM Glucose and 0.4 mM Palmitate, "Glc/Pal") media for 20 h. (bio-rad-antibodies.com)
Genetic materials or proteins conveyed by EVs functionally change the recipient cells [ 13 ], and therefore visualization and tracking of EVs currently receive great attention as a way to reveal their physiology. (biomedcentral.com)

Anatomy39

Organisms10

Diseases6

Chemicals and Drugs145

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes36

Disciplines and Occupations1

Information Science3

Monoclonal Antibody4

Signal transduction3

Tetraspanin4

Membrane7

Antigen1

Intracellular3

Lipids3

Alexa Fluor1

Associated with Hermansky-Pudlak s2

Recombinant Protein3

Clone1

Platelets3

CD812

Nucleic acids1

Platelet activation1

Secretion2

RNAs1

Differentially expressed2

Binds2

Exosomes7

FITC2

Alix2

CD371

Proteomics1

Amino acids1

Glycoprotein2

Contain proteins1

IgG11

Receptor2

Biological1

Homolog1

Markers1

Molecules1

Mammalian1

Encodes1

Demonstrate1

Progression1

ELISA2

Western Blot3

Overlap1

Fibroblasts3

Molecular1

Exosomal1

Cells2