Mannose 6-phosphReceptorsLipidIonsResiduesKinasesMacromoleculesStructuralPhosphatidylinositolWater moleculesGuanineHeterotrimericDifferentiationPutativeMembranesMoleculesNucleicGenesSubunitsRegulatorsHomologyPurificationMolecularTarget proteinGeneAntibodiesNuclearCellAffinityAntibodyClassificationCommonlyTargetsSiteSimilarRegulationSitesAdditionSpeciesLargeOccurDiverseConcentrationCrystal structureFactorFamilyShowsDomainsBloodReleaseTotalMajor
Mannose 6-phosph2
- Mannose-6-phosphate receptor binding protein 1 (M6PRBP1) is a protein which in humans is encoded by the M6PRBP1 gene. (wikipedia.org)
- The mannose-6-phosphate receptor-binding properties of TIP47 are disputed, despite the designation of M6PRBP1 as TIP47's gene symbol. (wikipedia.org)
Receptors3
- Our structural and functional assays demonstrate the different binding modes of chemically distinct agonists of S1PRs, reveal the mechanical switch that activates these receptors, and provide a framework for understanding ligand selectivity and G protein coupling. (rcsb.org)
- Mannose 6-phosphate receptors (MPRs) deliver lysosomal hydrolase from the Golgi to endosomes and then return to the Golgi complex. (wikipedia.org)
- M6PRBP1 has been shown to interact with both Mannose 6-phosphate receptors. (wikipedia.org)
Lipid4
- TIP47 protein is most commonly described in the scientific literature as a coat protein for lipid droplets. (wikipedia.org)
- The perilipin family of structural lipid droplet proteins: stabilization of lipid droplets and control of lipolysis" (PDF). (wikipedia.org)
- The PX domain is a phosphoinositide (PI) binding module present in many proteins with diverse functions such as cell signaling, vesicular trafficking, protein sorting, and lipid modification, among others. (nih.gov)
- 1994). High-res- belongs to the family of intracellular lipid bind- olution crystal structures (Sacchetti ni et al. (lu.se)
Ions3
- Proteins that bind to and are involved in the metabolism of phosphate ions. (uams.edu)
- It binds to metal ions and may aid in the transport of copper. (sigmaaldrich.com)
- The chromatography medium provides very high binding capacity for histidine-tagged proteins and shows negligible leakage of Ni 2+ ions. (sigmaaldrich.com)
Residues2
- and binds plasminogen, via lysine residues. (leibniz-hki.de)
- Two basic residues are key in binding with phosphoinositides: one forms hydrogen bonds with the 3-phosphate of PI(3)P and another forms hydrogen bonds with the 4-and 5-hydroxyl groups of PI(3)P. (nih.gov)
Kinases4
- Ser/Thr protein kinases such as the Akt/Rac family, the beta-adrenergic receptor kinases, the mu isoform of PKC and the trypanosomal NrkA family. (embl.de)
- Tyrosine protein kinases belonging to the Btk/Itk/Tec subfamily. (embl.de)
- Screen Quest™ assay kits, a set of HTS-ready tools for high throughput screening of biochemical and cellular targets such as protein kinases, proteases, HDAC, cell apoptosis and cytoxicity, GPCR, ion channels, ADME/metabolism and transporters. (stratech.co.uk)
- it is involved in skeletal muscle contraction, excitation-contraction coupling in cardiac and smooth muscle, and activation of protein kinases and enzyme phosphorylation. (msdmanuals.com)
Macromolecules1
- It also has a role in binding of heme groups, macromolecules and phosphate groups in the binding sites of proteins. (sigmaaldrich.com)
Structural4
- Figure 5: Structural comparison of α-DG-binding LG domains. (nature.com)
- The structural comparison of binding sites is especially useful when applied on distantly related proteins as a comparison solely based on the amino acid sequence is not sufficient in such cases. (uni-marburg.de)
- Matching of structural motifs using hashing on residue labels and geometric filtering for protein function prediction. (uni-marburg.de)
- Moreover, the structure raises interesting possibilities about how type 2 secretion-system substrates may interact with the secretion machinery and demonstrates the utility of new artificial intelligence protein structure-prediction algorithms in making challenging structural targets tractable. (bvsalud.org)
Phosphatidylinositol2
Water molecules2
- A single glucuronic acid-β1,3-xylose disaccharide repeat straddles a Ca 2+ ion in the LG4 domain, with oxygen atoms from both sugars replacing Ca 2+ -bound water molecules. (nature.com)
- [ 1 , 2 ] PEG, a water-soluble linear polymer, appears to accelerate antibody-RBC binding by steric exclusion of water molecules in the diluents and to promote antibody detection. (medscape.com)
Guanine1
- Regulators of small G-proteins like guanine nucleotide releasing factor GNRP (Ras-GRF) (which contains 2 PH domains), guanine nucleotide exchange proteins like vav, dbl, SoS and Saccharomyces cerevisiae CDC24, GTPase activating proteins like rasGAP and BEM2/IPL2, and the human break point cluster protein bcr. (embl.de)
Heterotrimeric1
- Herein, we present five cryo-electron microscopy structures of S1PRs bound to diverse drug agonists and the heterotrimeric Gi protein. (rcsb.org)
Differentiation1
- IFABP and the binding thermodynamics (Richieri growth and differentiation (Glatz & van der et al. (lu.se)
Putative1
- Mouse protein citron, a putative rho/rac effector that binds to the GTP-bound forms of rho and rac. (embl.de)
Membranes3
- The PX domain is involved in targeting of proteins to PI-enriched membranes, and may also be involved in protein-protein interaction. (nih.gov)
- Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways. (embl.de)
- a sensitive and robust tool for detection of proteins in solutions and solid surfaces (such as gels and membranes). (stratech.co.uk)
Molecules1
- These programs are utilized to screen entire libraries of molecules for a possible ligand of a binding site and to furthermore estimate in which conformation the ligand will most likely bind. (uni-marburg.de)
Nucleic1
- iFluor™ superior fluorescent labelling dyes, optimised for labelling proteins and nucleic acids. (stratech.co.uk)
Genes1
- TIP47 belongs to the peripilin protein family and shares significant homology with the other genes of this family, including perilipin and adipophilin. (wikipedia.org)
Subunits1
- Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
Regulators4
- Candida albicans uses human complement regulators such as factor H and factor H-like protein 1 (FHL-1) for immune evasion. (leibniz-hki.de)
- The 3 human complement regulators, when attached to candida Gpd2, became functionally active, and the attached host proteins assist in inactivation of the complement cascade or cleave fibrinogen in the extracellular matrix component fibrinogen. (leibniz-hki.de)
- Thus, candida Gpd2 is a novel fungal immune evasion protein that binds several human complement regulators and, in addition, binds human cells. (leibniz-hki.de)
- The canonical TGFβ signalling pathway involves ligand-dependent assembly of a heteromeric receptor complex, receptor-kinase activation and subsequent phosphorylation and activation of SMAD proteins, which are transcriptional regulators that consequently accumulate in the nucleus. (nature.com)
Homology1
- Pleckstrin homology (PH) domains are small modular domains that occur in a large variety of proteins. (embl.de)
Purification1
- To prevent the binding of host cell proteins with exposed histidines, it is essential to include imidazole at a low concentration in the sample and binding buffer ( Optimizing purification of histidine-tagged proteins ). (sigmaaldrich.com)
Molecular1
- A knowledge gap in understanding of the T2SS is the molecular role of a small "pseudopilin" protein. (bvsalud.org)
Target protein2
- Binding sites of other proteins which are similar to the binding site of the target protein can offer hints for possible side effects of a new drug prior to clinical studies. (uni-marburg.de)
- 500 mM imidazole in the elution buffer ensures complete elution of the target protein. (sigmaaldrich.com)
Gene1
- The protein encoded by this gene interacts with the cytoplasmic domains of both cation-independent and cation-dependent MPRs, and is required for endosome-to-Golgi transport. (wikipedia.org)
Antibodies1
- The AHG detects bound RBC antibodies that do not produce direct agglutination (sensitizing antibody). (medscape.com)
Nuclear1
- Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
Cell2
- Several S. cerevisiae proteins involved in cell cycle regulation and bud formation like BEM2, BEM3, BUD4 and the BEM1-binding proteins BOI2 (BEB1) and BOI1 (BOB1). (embl.de)
- The presence of agglutination with the addition of AHG indicates antibody binding to a specific red cell antigen. (medscape.com)
Affinity2
- The interaction with RAB9 has been shown to increase the affinity of this protein for its cargo. (wikipedia.org)
- The chelating binding mode accounts for the high affinity of this protein-carbohydrate interaction. (nature.com)
Antibody1
- AHG is an animal antibody that binds to the F c portion of human immunoglobulin. (medscape.com)
Classification1
- Pocketome via Comprehensive Identification and Classification of Ligand Binding Envelopes. (uni-marburg.de)
Commonly1
- Domain commonly found in eukaryotic signalling proteins. (embl.de)
Targets1
- These models are being used to systematically elucidate proteins required for cutaneous carcinogenesis and to test their potential role as therapeutic targets. (stanford.edu)
Site2
- Protein binding site comparisons are frequently used receptor-based techniques in early stages of the drug development process. (uni-marburg.de)
- Moreover, binding site comparisons are used as an idea generator for bioisosteric replacements of individual functional groups of the newly developed drug and to unravel the function of hitherto orphan proteins. (uni-marburg.de)
Similar2
- A Geometric Algorithm to Find Small but Highly Similar 3D Substructures in Proteins. (uni-marburg.de)
- For linear gradient elution, a shallow gradient, over 20 column volumes, may separate proteins with similar binding strengths. (sigmaaldrich.com)
Regulation1
- Constam, D. B. & Robertson, E. J. Regulation of bone morphogenetic protein activity by pro domains and proprotein convertases. (nature.com)
Sites6
- In the present work, accelerated methods for the comparison of protein binding sites as well as an extended procedure for the assessment of ligand poses in protein binding sites are presented. (uni-marburg.de)
- Methods for the assessment of ligand poses in protein binding sites are also used in the early phase of drug development within docking programs. (uni-marburg.de)
- How to Measure the Similarity Between Protein Ligand-binding Sites. (uni-marburg.de)
- Efficient Similarity Retrieval for Protein Binding Sites based on Histogram Comparison. (uni-marburg.de)
- The Automatic Search for Ligand Binding Sites in Proteins of Known Three-dimensional Structure Using only Geometric Criteria. (uni-marburg.de)
- A simple and fuzzy method to align and compare druggable ligand-binding sites. (uni-marburg.de)
Addition1
- In addition, candida Gpd2 bound to human nonphagocytic cells but not to phagocytic U937 cells. (leibniz-hki.de)
Species1
- The heavy chain is responsible for both receptor some species of the genus Clostridium, in particular, Clostridium binding via its C-terminal (CT) binding domain [4,5] (HC) and botulinum, C. butyricum, C. baratii, and C. argentinense. (cdc.gov)
Large2
- Like-acetylglucosaminyltransferase (LARGE) synthesizes the matrix-binding heteropolysaccharide [-glucuronic acid-β1,3-xylose-α1,3-] n . (nature.com)
- The atomic details of matrix binding were revealed by a high-resolution crystal structure of laminin-G-like (LG) domains 4 and 5 (LG4 and LG5) of laminin-α2 bound to a LARGE-synthesized oligosaccharide. (nature.com)
Occur1
- Additionally, the structure of CbpD shows that post-translational modifications occur on the chitin-binding surface. (bvsalud.org)
Diverse1
- Pseudomonas aeruginosa secretes diverse proteins via its type 2 secretion system, including a 39â kDa chitin-binding protein, CbpD. (bvsalud.org)
Concentration1
- The optimal concentration of imidazole needed in the sample and buffer to obtain the best purity and yield differs from protein to protein. (sigmaaldrich.com)
Crystal structure1
- Figure 3: Crystal structure of laminin-α2 LG4-5 bound to oligosaccharide G6/7. (nature.com)
Factor1
- Glycerol-3-phosphate dehydrogenase 2 is a novel factor H-, factor H-like protein 1-, and plasminogen-binding surface protein of Candida albicans. (leibniz-hki.de)
Family2
- This protein also binds directly to the GTPase RAB9 (RAB9A), a member of the RAS oncogene family. (wikipedia.org)
- The domain family possesses multiple functions including the abilities to bind inositol phosphates, and various proteins. (embl.de)
Shows1
- This graph shows the total number of publications written about "Phosphate-Binding Proteins" by people in UAMS Profiles by year, and whether "Phosphate-Binding Proteins" was a major or minor topic of these publications. (uams.edu)
Domains2
Blood1
- Red blood cells (RBCs) carry numerous protein and carbohydrate antigens on their surface. (medscape.com)
Release2
Total1
- Total calcium (ie, protein-bound, complexed, and ionized calcium) is usually what is determined by clinical laboratory measurement. (msdmanuals.com)
Major1
- Pleckstrin, the protein where this domain was first detected, is the major substrate of protein kinase C in platelets. (embl.de)