Protein binds subunit ribosomal proteins. Medical search. Frequent questions

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. (ucsc.edu)
In bacterial ribosomes, the small (30S) ribosomal subunit is composed of 16S rRNA and 21 distinct proteins. (unboundmedicine.com)
Ribosomes, the site of protein synthesis, are a major target for natural and synthetic antibiotics. (proteopedia.org)
The vast majority of proteins entering the secretory pathway are synthesized on ribosomes docked at ER translocons and are co-transationally translocated into the ER lumen. (stanford.edu)
Ribosomes are the particles that catalyse mRNA-directed protein synthesis in all organisms. (embl.de)

Doxycycline inhibits bacterial protein synthesis by binding to the 30S ribosomal subunit. (nih.gov)
For the sake of energy preservation, bacteria, upon transition to stationary phase, tone down their protein synthesis. (nih.gov)
This is achieved by binding tRNA to the bacterial ribosomal subunit, blocking the initiation of protein synthesis. (internationaldrugmart.com)
The L16 ribosomal protein of the 50S or its N-terminal fragment are required for EF-P mediated peptide bond synthesis, whereas L11, L15, and L7/L12 are not required in this reaction, suggesting that EF-P may function at a different ribosomal site than most other translation factors. (embl-heidelberg.de)
Our goal is to elucidate the functional networks that coordinate protein synthesis and quality control in the early secretory pathway. (stanford.edu)
It works by inhibiting the synthesis of essential proteins in bacteria, thereby preventing their growth and reproduction. (tangorm.com)
While the crucial activities of decoding and peptide transfer are RNA based, proteins play an active role in functions that may have evolved to streamline the process of protein synthesis. (embl.de)
Pharmacology - Tobramycin, like the other aminoglycoside antibiotics, act on susceptible bacteria presumably by irreversibly binding to the 30S ribosomal subunit thereby inhibit-ing protein synthesis. (elephantcare.org)

The protein binds 5S rRNA to form a stable complex. (wikipedia.org)
In turn 5S rRNA binds specifically to three proteins, L25, L18 and L5, forming a separate domain of the bacterial ribosome. (wikipedia.org)
Ribosomal protein S15 is of particular interest because it binds primarily to 16S rRNA and is required for assembly of the small subunit and for intersubunit association, thus representing a key element in the assembly of a whole ribosome. (unboundmedicine.com)
It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome (By similarity). (proteopedia.org)
HAMAP-Rule:MF_01331_B] [ RL4_DEIRA ] One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. (proteopedia.org)
HAMAP-Rule:MF_01328_B] Makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit. (proteopedia.org)
EF-P binds near the streptomycine binding site of the 16S rRNA in the 30S subunit. (embl-heidelberg.de)
Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. (joplink.net)
Usually they decorate the rRNA cores of the subunits. (embl.de)
Many ribosomal proteins, particularly those of the large subunit, are composed of a globular, surfaced-exposed domain with long finger-like projections that extend into the rRNA core to stabilise its structure. (embl.de)
In the large subunit, about 1/3 of the 23S rRNA nucleotides are at least in van der Waal's contact with protein, and L22 interacts with all six domains of the 23S rRNA. (embl.de)
Proteins S4 and S7, which initiate assembly of the 16S rRNA, are located at junctions of five and four RNA helices, respectively. (embl.de)
In this way proteins serve to organise and stabilise the rRNA tertiary structure. (embl.de)
Ribosomal protein L31e, which is present in archaea and eukaryotes, binds the 23S rRNA and is one of six protein components encircling the polypeptide exit tunnel. (embl.de)
It is an essential nucleolar protein involved in processing and maturation of 27S pre-rRNA and biogenesis of 60S ribosomal subunits. (nih.gov)

50S ribosomal protein L25 is a protein that in Escherichia coli is encoded by the rplY gene. (wikipedia.org)
The Obg protein in Escherichia coli (ObgE) has been implicated in many diverse cellular functions, with proposed molecular roles in two global processes, ribosome assembly and stringent response. (rcsb.org)
In Escherichia coli, S4 is known to bind directly to 16S ribosomal RNA. (expasy.org)

A number of eukaryotic and archaebacterial large subunit ribosomal proteins can be grouped on the basis of sequence similarities. (embl.de)

As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (ucsc.edu)
Genes upregulated in the treated B. burgdorferi included a number of Erp genes and rplU , a 50S ribosomal protein. (frontiersin.org)

HAMAP-Rule:MF_01331_B] The globular domain of the protein is located by the polypeptide exit tunnel on the outside of the subunit while an extended beta-hairpin forms part of the wall of the tunnel. (proteopedia.org)
In addition to globular domains, many of the proteins have long, extended regions, either in the termini or in internal loops, which make extensive contact to the RNA component and are involved in stabilizing RNA tertiary structure. (caltech.edu)
Analysis of the protein-RNA interactions in the context of ribosomal assembly shows that the primary binders are globular proteins that bind at RNA multihelix junctions, whereas proteins with long extensions assemble later. (caltech.edu)

A phylogenetically conserved ribosomal protein L16p/L10e organizes the architecture of the aminoacyl tRNA binding site on the large ribosomal subunit. (rcsb.org)
The codons of the mRNA are exposed on the ribosome to allow tRNA binding. (embl.de)

3] So-called breast milk allergy is a status of food allergy induced in breastfed babies by heterologous proteins (typically cow's milk proteins) ingested by their mothers and appearing in their breast milk. (medscape.com)
Therefore, it became apparent that each of these fungi possesses considerable potential as expression hosts for producing numerous small molecules and heterologous proteins 14 . (nature.com)

Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. (ucsc.edu)

This highly complex 'protein biogenesis' process is assisted by a diverse network of folding catalysts and protein-modifying enzymes and is scrutinized by molecular chaperones and other 'quality control' factors which ensure that only correctly folded and assembled proteins exit the ER and proceed to distal compartments of the secretory pathway. (stanford.edu)

To elucidate the structural basis of ribosome-antibiotic interactions, we determined the high-resolution X-ray structures of the 50S ribosomal subunit of the eubacterium Deinococcus radiodurans, complexed with the clinically relevant antibiotics chloramphenicol, clindamycin and the three macrolides erythromycin, clarithromycin and roxithromycin. (proteopedia.org)

We present a detailed analysis of the protein structures in the 30 S ribosomal subunit from Thermus thermophilus, and their interactions with 16 S RNA based on a crystal structure at 3.05 Å resolution. (caltech.edu)

This gene encodes a ribosomal protein that is a component of the 60S ribosome subunit. (nih.gov)
however, these fusions actually involve a ribosomal protein L22 pseudogene located at 3q26, and this gene actually maps to 1p36.3-p36.2. (ucsc.edu)
KJ892031 - Synthetic construct Homo sapiens clone ccsbBroadEn_01425 RPL22 gene, encodes complete protein. (ucsc.edu)

A cation-binding pocket formed between alpha2 and beta 6 is similar to that of the archaeal L10 protein but appears to be better ordered. (rcsb.org)

One example is the conserved bacterial ribosome silencing factor (RsfS) that binds to uL14 protein onto the large ribosomal subunit and prevents its association with the small subunit. (nih.gov)
Here we describe the binding mode of Staphylococcus aureus RsfS to the large ribosomal subunit and present a 3.2 Å resolution cryo-EM reconstruction of the 50S-RsfS complex together with the crystal structure of uL14-RsfS complex solved at 2.3 Å resolution. (nih.gov)
We attempt to correlate the structure with a large body of biochemical and genetic data on the 30 S subunit. (caltech.edu)
The proteins are named in accordance with the subunit of the ribosome which they belong to - the small (S1 to S31) and the large (L1 to L44). (embl.de)

Tetracyclines are readily absorbed and are bound to plasma proteins in varying degree. (nih.gov)
20%) to plasma proteins. (elephantcare.org)

Protein S15 interacts in the minor groove with a G-U/G-C motif and a three-way junction. (unboundmedicine.com)
HAMAP-Rule:MF_01328_B] This protein is located close to the polypeptide exit tunnel, and interacts with the modified macrolide azithromycin, which blocks the tunnel. (proteopedia.org)

Here, using pre-steady state fast kinetics we demonstrate that ObgE is an anti-association factor, which prevents ribosomal subunit association and downstream steps in translation by binding to the 50S subunit. (rcsb.org)

ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)

BC058887 - Homo sapiens ribosomal protein L22, mRNA (cDNA clone MGC:64893 IMAGE:6141451), complete cds. (ucsc.edu)
X59357 - Human mRNA for Epstein-Barr virus small RNAs (EBERs)associated protein (EAP). (ucsc.edu)
Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)

Nop4p has four RNA recognition motifs (RRMs), also termed RBDs (RNA binding domains) or RNPs (ribonucleoprotein domains). (nih.gov)

Yeast protein NAM9 [ 3 ]. (expasy.org)
Yeast ribosomal proteins: XII. (expasy.org)

These nucleic acid binding domains are predominantly found in elongation factor P, where they adopt an OB-fold, with five beta-strands forming a beta-barrel in a Greek-key topology ( PUBMED:15210970 ). (embl-heidelberg.de)

Many ribosomal proteins share similar α+β sandwich folds, but we show that the topology of this domain varies considerably, as do the ways in which the proteins interact with RNA. (caltech.edu)

Doxycycline has a high degree of lipoid solubility and a low affinity for calcium binding. (rxlist.com)

Replication of bacteria happens on the ribosome, and medications belonging to the category of tetracyclines prevent translation initiation of proteins. (internationaldrugmart.com)
It belongs to a class of antibiotics called tetracyclines and works by preventing the bacteria from producing proteins essential for their survival. (tangorm.com)

Ribosomal protein L10 in mitochondria serves as a regulator for ROS level in pancreatic cancer cells. (nih.gov)

AK097075 - Homo sapiens cDNA FLJ39756 fis, clone SMINT2018681, moderately similar to 60S RIBOSOMAL PROTEIN L22. (ucsc.edu)
Membrane proteins must be threaded co-translocationally into the lipid bilayer to become membrane-integrated, often with complex topologies and typically form hetero- or homo- oligomers. (stanford.edu)

Image analysis of the matched maps identified 7 proteins that were either over- or down-expressed: activated protein kinase c receptor (LACK), alpha tubulin (×2), prostaglandin f2-alpha synthase, protein disulfide isomerase, vesicular transport protein and a hypothetical protein. (who.int)

NAM9 nuclear suppressor of mitochondrial ochre mutations in Saccharomyces cerevisiae codes for a protein homologous to S4 ribosomal proteins from chloroplasts, bacteria, and eucaryotes. (expasy.org)

There are 17994 Elong-fact-P_C domains in 17994 proteins in SMART's nrdb database. (embl-heidelberg.de)
Most of the proteins interact with multiple RNA elements, often from different domains. (embl.de)

The latter is constrained by a conserved base triple and stacking interactions, and locked into place by magnesium ions and protein side chains, mainly through interactions with the unique three-dimensional geometry of the backbone. (unboundmedicine.com)
Here we report the details of antibiotic interactions with the components of their binding sites. (proteopedia.org)
With 20 different polypeptide chains, the 30 S subunit adds significantly to our data base of RNA structure and protein-RNA interactions. (caltech.edu)

The protein belongs to the L22E family of ribosomal proteins. (ucsc.edu)
Obg proteins are a family of P-loop GTPases, conserved from bacteria to human. (rcsb.org)

These immundominant allergens were identified by nanoscale UPLC-MS/MS to be 60S ribosomal protein L10 (25 kDa), electron transfer flavoprotein B subunit (29 kDa), ketol acid reductoisomerase (44 kDa), and heat shock protein 70 (65 kDa). (cdc.gov)

Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20? (joplink.net)

We found that antibiotic binding sites are composed exclusively of segments of 23S ribosomal RNA at the peptidyl transferase cavity and do not involve any interaction of the drugs with ribosomal proteins. (proteopedia.org)

however, upon binding to guanosine tetraphosphate (ppGpp), the global regulator of stringent response, ObgE exhibits an enhanced interaction with the 50S subunit, resulting in increased equilibrium dissociation of the 70S ribosome into subunits. (rcsb.org)

Detailed knowledge of antibiotic binding sites is central to understanding the mechanisms of drug action. (proteopedia.org)

Anatomy4

Organisms2

Diseases1

Chemicals and Drugs16

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Phenomena and Processes17

Information Science3

Ribosomes5

Synthesis8

RRNA15

Escherichia3

Basis of sequence similarities1

Genes2

Globular3

TRNA2

Heterologous proteins2

Distinct proteins1

Biogenesis1

Eubacterium1

Thermus1

Gene3

Archaeal1

Large4

Plasma proteins2

Interacts2

Prevents1

Abstract1

MRNA3

Motifs1

Yeast2

Nucleic acid1

Folds1

Doxycycline1

Tetracyclines2

Mitochondria1

Homo2

Disulfide isomerase1

Saccharomyces1

Domains2

Interactions3

Family2

Electron1

Sterile1

Involve1

Exhibits1

Antibiotic1