Protein binds interleukin receptor clone cells. Medical search. Frequent questions

Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. (thermofisher.com)
The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. (thermofisher.com)
Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. (thermofisher.com)
IL-11 exerts its biological activities through binding to a specific high-affinity receptor having an apparent molecular mass of 150 kDa. (rndsystems.com)
The encoded protein is secreted and binds hydroxyapatite with high affinity. (origene.com)
The functional high affinity IL-12 receptor is composed of at least IL12rb1 and IL12rb2. (lu.se)
These newly developed protein binders, showing high affinity towards their targets as well as keeping their initial binding to HSA, present another possibility to combine the advantages of small engineered scaffold proteins with those of typical larger proteins, allowing for more convenient production in bacteria leading to lower production costs and making them ideal candidates for future therapeutics. (kth.se)
IL-13 binds IL-13Rα1 with a minimal affinity and IL-4Rα binds to create a high-affinity cytokine-binding heterodimer then. (gasyblog.com)
IL-13Rα2 binds IL-13 specifically and with high affinity. (gasyblog.com)
Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. (wikidoc.org)
Fab m909 bound with relatively high affinity (equilibrium dissociation constant 57 nM) to FRβ. (biomedcentral.com)
Among the four isoforms of FRs identified (α, β, γ, and δ), α and β isoforms of FR are glycosylphosphatidylinositol (GPI)-anchored proteins with two N-glycosylation sites, and both have high affinity (K D of approximately 1 nM) for folate [ 2 ]. (biomedcentral.com)

Type I IL-4 receptor expression was increased on M1-like macrophages but did not lead to a differing ability of these cytokines to modulate M1-like macrophage superoxide production. (bvsalud.org)
Overall, this study did not identify major differences in the ability of IL-4 and IL-13 to modulate macrophage function, suggesting that the opposing roles of these cytokines in cardiovascular disease are likely to be via actions on other cell types. (bvsalud.org)
IL-1 alpha and IL-1 beta are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. (rndsystems.com)
IL-17 cytokines, receptors and signaling. (betalifesci.com)
Specific binding modules were achieved by performing phage display selections towards the targets Tumor necrosis factor alpha (TNF⍺) and Interleukin-17c (IL-17c), both proinflammatory cytokines involved in many different inflammatory diseases and therefore interesting targets for therapeutic applications. (kth.se)
The cytokines of a possible significance in that range include interleukin 17 family (IL-17) containing the group of IL-17A-F ligands and IL-17RA-E receptors. (czytelniamedyczna.pl)
Cytotoxins (perforin and granzyme) and cytokines (TNF-α and IFN-γ) were dramatically released from the BsAb-armed T cells after engaging cancer cells, resulting in a remarkable anti-cancer efficacy. (biomedcentral.com)

Moreover, treatment with imiquimod downregulated MHC class I molecules on tumor cells possibly resulting in a reduced binding affinity for inhibitory receptor NKG2A expressed on γδ T cells. (aacrjournals.org)
This CD86 antibody was made by transfecting human CD86 monoclonal antibody gene-vector clones into a cell line for in vitro expression, then purifying the antibody using affinity chromatography from the tissue culture supernatant (TCS). (cusabio.com)
The obtained antiserum was precipitated by saturated ammonium sulfate and then purified by Protein A affinity chromatography. (hindawi.com)
The CR1 receptor preferentially binds C3b that is covalently attached to immune complexes, and it has a weaker affinity for bound C4b and iC3b. (medscape.com)
To provide high-density cell cultures with sufficient iron, elevated concentrations of iron salts are required that utilize low-affinity nontransferrin receptor pathways. (genengnews.com)
Iron-bound transferrin has an increased affinity for the transferrin receptor, which is then transported into the cell via receptor-mediated endocytosis. (genengnews.com)
Furthermore, IL-17 family members exhibit binding affinity towards specific receptors, triggering downstream signaling pathways that regulate immune cell functions and immune responses. (betalifesci.com)
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (origene.com)
The S1 subunit (PDB I.D-6VSB) of the spike protein being reported to acts as receptor binding domain (RBD), binding to ACE-2 receptor with higher affinity. (ijpsonline.com)
Binds to IL-12 with a low affinity. (lu.se)
Therefore, a new combinatorial protein library based on ABD-derived affinity proteins (ADAPTs) was generated, originating from a bacterial albumin-binding domain. (kth.se)
The evident advantage of these fusion proteins and their competency to be functionally produced in E. coli as well as the possibility to avoid an additional step of conjugation or coupling of affinity proteins to cytotoxic payloads, makes this approach a promising alternative for current procedures and another reason why ESPs are on the rise. (kth.se)

The IL6 receptor is a protein complex consisting of an IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. (wikipedia.org)
The protein encoded by this gene is a subunit of the interleukin 13 receptor. (thermofisher.com)
This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. (thermofisher.com)
This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. (thermofisher.com)
Type I and II receptor subunit (IL-2RÎ³, IL-4Rα and IL-13Rα1) and M2 marker (MRC-1, CCL18, CCL22) expression was assessed via RT-qPCR in IL-4- and IL-13-treated human primary macrophages. (bvsalud.org)
The study incorporates disciplines such as 5-HT5A receptor, Interleukin 5 receptor alpha subunit, Transfection and Interleukin 10 receptor, alpha subunit in addition to Molecular biology. (research.com)
However in the bleomycin model of lung fibrosis a controversial part for the IL-13Rα2 subunit was proposed which suggested that activation of this receptor led to induction of TGF-β and the development of lung fibrosis [18]. (gasyblog.com)
The protein level of TLR4 was significantly increased and the ratio of phosphorylated NF-κB p65 versus p65 subunit was also elevated in PM 2.5 group. (medsci.org)
B29), and the -subunit of the E-mail: [email protected] interleukin (IL)-7 receptor [5, 6]. (lu.se)

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. (cusabio.com)
IL-15 regulates T and natural killer cell activation and proliferation. (thermofisher.com)
IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2. (thermofisher.com)
Measured by its ability to neutralize IL‑11-induced proliferation in the T11 mouse plasmacytoma cell line. (rndsystems.com)
Cell Proliferation Induced by IL‑11 and Neutralization by Human IL‑11 Antibody. (rndsystems.com)
Recombinant Human IL-11 (Catalog # 218-IL ) stimulates proliferation in the T11 mouse plasmacytoma cell line in a dose-dependent manner (orange line). (rndsystems.com)
These members actively participate in the activation of immune cells, production of inflammatory mediators, and facilitate processes such as immune cell proliferation and tissue repair. (betalifesci.com)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
We found that the expanded cells possessed significantly improved immune effector functions, including proliferation, differentiation, and cancer cell killing, both in vitro and in the humanized mouse model. (nature.com)
Tyrosine 343 in the erythropoietin receptor positively regulates erythropoietin‐induced cell proliferation and Stat5 activation. (sagepub.com)
The EP4 receptor antagonist, L-161,982, blocks prostaglandin E2-induced signal transduction and cell proliferation in HCA-7 colon cancer cells. (research.com)
Their role as the factors directly influencing not only growth and proliferation of cancer cells, but also as the factors modulating their apoptosis or regulating neoangiogenesis process is demonstrated. (czytelniamedyczna.pl)
The anti-CD3 moiety of the BsAb bound to the T cell surface and stimulated the differentiation and proliferation of T cells in PBMCs. (biomedcentral.com)
We hypothesized that, in CBD, failure of IL-10 to modulate the beryllium-specific, cell-mediated immune response would result in persistent, maximal cytokine production and T lymphocyte proliferation, thus contributing to the development of granulomatous lung disease. (cdc.gov)
bronchoalveolar lavage cells from control and CBD subjects to evaluate the beryllium salt-specific production of endogenous IL-10 and the effects of exogenous human rIL-10 (rhIL-10) on HLA expression, on the production of IL-2, IFN- , and TNF- , and on T lymphocyte proliferation. (cdc.gov)
We of both mitogen- and Ag-induced accessory cell-dependent T lym- phocyte proliferation. (cdc.gov)
They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. (bvsalud.org)

Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation. (cusabio.com)
This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. (thermofisher.com)
Receptor-interacting protein kinase 1 (RIPK1) is a key component of the tumor necrosis factor (TNF) receptor signaling complex that regulates both pro- and anti-apoptotic signaling. (mdpi.com)
It has been reported that RNF4 negatively regulates TNF-α-induced activation of the nuclear factor-κB (NF-κB) through downregulation of transforming growth factor β-activated kinase 1 (TAK1) activity, indicating the possibility that RNF4-mediated TAK1 suppression results in enhanced sensitivity to cell death. (mdpi.com)
Molecular cloning of L-JAK, a Janus family protein-tyrosine kinase expressed in natural killer cells and activated leukocytes. (sagepub.com)
His Molecular biology research integrates issues from Alpha, Gene expression, Complementary DNA, Protein kinase C and Gene isoform. (research.com)
His work carried out in the field of Receptor brings together such families of science as Prostaglandin E, Prostaglandin and Kinase. (research.com)
This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. (nih.gov)
This protein kinase is calcium-independent and phospholipid-dependent. (nih.gov)
This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. (nih.gov)
Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes. (jci.org)
Glucose has been reported to increase the de novo synthesis of diacylglycerol (DAG) and translocate and activate protein kinase C (PKC) in rat adipocytes. (jci.org)

Adenoviral-mediated delivery of a viral chemokine binding protein blocks CC-chemokine activity in vitro and in vivo. (ox.ac.uk)
Although still allowed in France, Diaclone decided to replace this in vivo production process with an entirely in vitro cell culture method, an alternative technology with an equivalent cost to the old ascitic method but also with higher reproducibility and quality. (diaclone.com)
The large majority of clones are produced by in vitro methods, hybridoma cell cultures or recombinant technologies. (diaclone.com)
The precursor protein (pre-IL-33) was cleaved by caspase-1 into a mature 18-kDa protein (IL-33) in in vitro experiments using a recombinant protein [ 2 ]. (hindawi.com)
Consistent with the in vitro functions of IL‑11, in vivo administration of rhIL-11 in normal mice was found to enhance the generation of Ig producing cells and platelets, and to increase the cycling rates of bone marrow-derived CFU-GM, BFU-E, and CFU-GEMM progenitors. (rndsystems.com)
An in vitro technique is described for assessing the chemotactic activity of soluble substances on motile cells. (typeset.io)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
To apply allogeneic Vγ9Vδ2 T cells in adoptive immunotherapy, the methodology used to obtain adequate cell numbers with optimal effector function in vitro needs to be optimized, and clinical safety and efficacy also need to be proven. (nature.com)
For in vivo test: In vitro lifestyle and embryo freezing After IVF, oocytes encircled with cumulus cells had been placed in fresh new TCM-199 moderate, as well as the embryos had been co-cultured with cumulus cells, as previously defined [21]. (bioskinrevive.com)
Each binder was then examined for their usefulness as a real therapeutic by successfully evaluating its ability to block the interaction of the cytokine and its specific receptor in vitro . (kth.se)
After proving these new fusion proteins have similar binding kinetics to the target, as well as comparable enzymatic activities, as their free counterpart, the cytotoxic effects were put to the test in vitro . (kth.se)
Antibody-dependent cell-mediated cytotoxicity (ADCC) was evaluated with FRβ positive CHO cells as target cells and isolated peripheral blood monocytes as effector cells in an in vitro assay. (biomedcentral.com)
PM 2.5 decreased the protein level of SIRT1 but not the mRNA expression in vitro and in vivo experiments. (medsci.org)

These professional antigen-presenting cells are able to discriminate unmodified self from non-self thanks to pattern recognition receptors such as the Toll like Receptors (TLR) and Scavenger Receptors (SR). SR were originally identified by their ability to bind and internalize modified lipoproteins and microorganisms but also particles and TLR agonists. (biomedcentral.com)
DC are professional antigen-presenting cells that are essential for initiating adaptive immune responses. (biomedcentral.com)
It is suggested that the reaction due to cell injury is similar to that due to antibody-antigen interaction because damage to cells causes the local formation of antibody-antigen complexes. (typeset.io)
THE local humoral and cellular events which occur in the skin following cell injury are very similar to those which follow the intradermal injection of soluble antigen in an immunized animal which has antibodies in its circulation. (typeset.io)
The usual explanation of this similarity is that the antibody-antigen reaction causes a certain degree of cell damage, with consequent release of the pharmacologically active substances which are also liberated following injury to cells from other causes. (typeset.io)
γδ T cells, specifically the Vγ9Vδ2 subset, which is the dominate subset among γδ T cells in human peripheral blood, recognize target cells in a human leukocyte antigen (HLA)-independent manner. (nature.com)
Docking results of the multi-epitope vaccine peptide with human leukocyte antigen class I and II alleles predicted efficient binding and the resulted docked models were stable during simulation. (ijpsonline.com)
Functional recombinant FRβ protein was produced in insect cells and used as antigen to isolate a mAb, m909, from a human naïve Fab phage display library. (biomedcentral.com)
The binding of specific T cell receptors (TCR) to major histocompatibility complex (MHC)/antigen complexes on cancer cells induces the TCR/CD3-mediated signal transduction pathway and stimulates the release of specific toxins (perforin and granzyme) for cancer elimination [ 6 , 7 ]. (biomedcentral.com)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)
If the mouse gene for only the antigen-binding hypervariable regions of the variable region is used, the product is termed "humanized. (msdmanuals.com)
Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
The evidence for an autoimmune etiology includes pathological findings of infiltrating T cells, the presence of antigen-antibody complexes in affected cartilage, cellular and humoral responses against collagen type II and other collagen antigens, and the observation that immunosuppressive regimens most often suppress the disease. (medscape.com)

CD86, also known as B7-2, is a transmembrane glycoprotein that is typically found on APCs such as macrophages, dendritic cells, and B cells. (cusabio.com)
However, chemotaxis of both CCR5-transfected cells and primary macrophages was inhibited by more than 90% by plasma from Ad35K-infected animals compared with control plasma from animals injected with AdGFP. (ox.ac.uk)
In addition to their action on macrophages and airway epithelial cells, DEP also modulate the functions of dendritic cells (DC). (biomedcentral.com)
DEP exert immunoregulatory functions through their first action on resident cells in the lung including macrophages, airway epithelial cells, and dendritic cells (DC). (biomedcentral.com)
Whereas alveolar macrophages mainly reside in the alveolar region of the lung, immature myeloid DC (mDC) constitute a dense network in close proximity to airway epithelial cells [ 13 ]. (biomedcentral.com)
Therefore, we co-cultured AML cells with macrophages. (frontiersin.org)
Macrophages are the most abundant white blood cells in the immune microenvironment and have a high degree of heterogeneity. (frontiersin.org)
Sections were first stained by indirect immunoperoxidase and numbers of monocytes/macrophages (MO/MA), T cells, B cells, and NK cells counted. (biomedcentral.com)
Studies have indicated that FRβ is over-expressed in activated macrophages in autoimmune diseases and some cancer cells. (biomedcentral.com)
m909 was not only able to select FRβ positive, activated macrophages from synovial fluid cells of arthritis patients as efficiently as folate, but also able to mediate ADCC in FRβ positive cells. (biomedcentral.com)

Eosinophils are a prominent feature in the pulmonary inflammation that is associated with allergic airway diseases, suggesting that inhibition of interleukin-5 is a viable treatment. (biomedcentral.com)
Importantly, inhibition of miR-146a-3p upregulated SIRT1 protein and suppressed M1 polarization in PM 2.5 treated RAW264.7 cells. (medsci.org)

Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. (wikipedia.org)
The T helper (Th) 2 immune response mediated by interleukin (IL)-4/13 is generally believed to be central in the pathogenesis of AD. (bvsalud.org)
It has been shown that innate lymphoid cells (ILCs) play a major effector cell role in the immune response in tissue homeostasis and inflammation and fascinating details about the interaction between innate and adaptive immunity. (bvsalud.org)
The tumor microenvironment of malignant gliomas is usually immunosuppressive,22 and this has been shown after CAR T?cell infusion.10 Immune checkpoint receptors (e.g. (campaignfornonviolentschools.org)
Susceptibility to infection in humans is strongly heritable, and among the many loci that influence it, those that encode proteins vital to the innate immune response are of central importance. (mhmedical.com)
Complement and complement receptors play a critical role in immune defense by initiating the rapid destruction of invading microorganisms, amplifying the innate and adaptive immune responses, and mediating solubilization and clearance of immune complexes. (medscape.com)
Defects in the expression of complement or complement receptors may result in loss of tolerance to self-proteins and the development of immune complex-mediated autoimmune diseases such as systemic lupus erythematosus (SLE). (medscape.com)
They tend to enhance the effects of complement and are highly important in the binding of opsonized immune complexes on B cells. (medscape.com)
Opsonized immune complexes (coated by C3b and C4b) bind to CR1, mostly on red blood cells, and are cleared through the liver where they can be transferred to CR3-bearing phagocytes and endocytosed. (medscape.com)
CR1 and CR2 have been shown to influence the immune environment in a B-cell receptor-independent manner. (medscape.com)
They actively promote inflammatory responses, facilitate the recruitment and activation of inflammatory cells, and contribute to the development and progression of immune-related diseases like autoimmune disorders, allergic reactions, and infections. (betalifesci.com)
The question proposed in the chapter is whether the postulated recognition factors of the immune system are detectable in serum or are attached to certain cells as natural antibodies. (typeset.io)
KEGG enrichment and gene set enrichment analysis (GSEA) showed that a large number of genes were enriched in inflammatory immune-related pathways, such as the toll-like receptor signaling pathway. (frontiersin.org)
Spike glycoprotein (PDB Id: 6VSB) of the coronavirus was selected and analysed using immune epitope database server for prediction of potential immunogenic B and T-cell epitopes. (ijpsonline.com)
Population conservation studies of the selected protein and predicted epitopes were also performed using population conservancy analysis tool of immune epitope database resource. (ijpsonline.com)
In silico immune evaluation using C-ImmSim server showed that the peptide could concurrently elicit cell-mediated and humoral immune responses. (ijpsonline.com)
Immune simulation studies significantly anticipated high levels of IgM and IgG, T-helper, T-cytotoxic cells, INF-γ. (ijpsonline.com)
After that, we examined the result of cultured conditioned mass media (CM) of IVF embryos on splenic immune system cells and Madin-Darby bovine kidney (MDBK) cells with stably presented ISG15 promoter-reporter constructs. (bioskinrevive.com)
His G protein-coupled receptor research is multidisciplinary, incorporating perspectives in PELP-1 and Immune receptor. (research.com)
In addition to immune defects that affect IgE synthesis, defects of cell-mediated immunity have also been reported, consistent with impaired TH 1 responses. (medscape.com)
Binding of Fab and IgG1 m909 to FRβ was measured by ELISA, surface plasmon resonance, immune fluorescence staining, and flow cytometry. (biomedcentral.com)
Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)

Functional murine interleukin 6 receptor with the intracisternal A particle gene product at its cytoplasmic domain. (wikipedia.org)
In gastric cancer patients, tuft cell and ILC2 gene signatures predict worsening survival in intestinal-type gastric cancer where ~40% of the corresponding cancers show enriched co-existence of tuft cells and ILC2s. (bvsalud.org)
Checkpoint inhibitors also can be directly delivered by the adoptively transferred CAR T?cells via gene modification, which aims to reduce the adverse effects that can be caused by systemic delivery of checkpoint inhibitors.32, 34 Chitinase-IN-1 This is an additional strategy we will utilize to further liberate the function of IL-13R2 CAR T?cells in immunotherapy of malignant gliomas. (campaignfornonviolentschools.org)
In this study, the cloning and expression of mouse IL-33 gene in Escherichia coli (E. coli ), purification of recombinant protein, and generation of polyclonal antibody against IL-33 are described. (hindawi.com)
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. (origene.com)
In the same 12 months IL-13 was reported to direct cells towards Th2 pathway with induction of B cell production of IgE [2] and its gene position was mapped in close proximity to IL-4 on chromosome 5q 23-31 [1]. (gasyblog.com)
Mice that are homozygous for a targeted disruption of the LDL receptor gene (LDLR-/- mice) were fed a diet that contained 1.25% cholesterol, 7.5% cocoa butter, 7.5% casein, and 0.5% cholic acid. (jci.org)
However, the commercial production of cancer-specific T cells is often hampered by laborious cell culture procedures, the concern of retrovirus-based gene transfection, or insufficient T cell purity. (biomedcentral.com)
However, the gene transfer technology used in current CAR T cells primarily depends on retrovirus or lentivirus systems, which will randomly insert the CAR gene into the T cell genome and may pose a potential carcinogenic risk [ 19 , 20 ]. (biomedcentral.com)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

Interleukin 6 receptor (IL6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. (wikipedia.org)
At the C-terminal region of the cell membrane, a conserved sequence called SEFIR defines the IL-17R family as a novel cytokine receptor family. (betalifesci.com)
Defective lymphoid development in mice lacking expression of the common cytokine receptor gamma chain. (sagepub.com)
The molecular basis of X-linked severe combined immunodeficiency: defective cytokine receptor signaling. (sagepub.com)

The reconstituted cells were then washed and stained with BD Horizon™ BUV395 Mouse Anti-Human CD4 antibody (Cat. (bdbiosciences.com)
The CD86 recombinant monoclonal antibody is a highly specific antibody that recognizes the human protein CD86. (cusabio.com)
Our findings suggest a role for ILC2 and tuft cells, along with their associated cytokine IL13 and IL25 as gatekeepers and enablers of metaplastic transformation and gastric tumorigenesis, thereby providing an opportunity to therapeutically inhibit early-stage gastric cancer through repurposing antibody-mediated therapies. (bvsalud.org)
With over 30 years of experience Diaclone is respected for its excellence in Monoclonal Antibody development and our highly respected B-clones ('B-' for Besancon) are utilized across the Life Science industry in research, clinical and discovery applications by research, pharmaceutical and manufacturing organizations worldwide. (diaclone.com)
Only a few monoclonal antibody clones with better specificity & productivity from ascites production have been conserved in the catalogue, in accordance with the strict regulations of veterinary agencies. (diaclone.com)
The prepared antibody can be useful for the study of expression and distribution of IL-33 in various tissues at protein level and for the elucidation of its biofunctions and regulation mechanism in inflammatory diseases. (hindawi.com)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TACE with 1131 at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). (qedbio.com)
In this study, we developed a non-genetic engineering technology for rapidly manufacturing a large amount of cancer-specific T cells by utilizing a unique anti-cancer/anti-CD3 bispecific antibody (BsAb) to directly culture human peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
CAR comprises an extracellular, cancer-specific antibody fused with the intracellular signaling activation domains of CD3 and co-activation ligands, such as CD28 or 4-1BB [ 17 , 18 ], which provide engineered T cells with an MHC‐independent mechanism through which to kill cancer cells directly. (biomedcentral.com)

The interaction of CD54 and the corresponding ligand CD11a/CD18 expressed on γδ T cells is responsible for triggering effector function in γδ T cells. (aacrjournals.org)
Effector proteins interacting with the Fc portion of immunoglobulin M (IgM) include complement and complement receptors. (medscape.com)
Unlike folate-drug conjugates, m909 selectively binds to FRβ, does not recognize FRα, and has at least one effector function. (biomedcentral.com)
Collectively, the BsAb-armed T cell technology represents a simple, time-saving, and highly safe method to generate highly pure cancer-specific effector T cells, thereby providing an affordable T cell immunotherapy to patients. (biomedcentral.com)
However, most effector T cells generated using these established cultivation methods lack cancer specificity [ 8 ]. (biomedcentral.com)

IL-1 is a name that designates two proteins, IL-1 alpha and IL-1 beta, that are the products of distinct genes, but recognize the same cell surface receptors. (rndsystems.com)
Here, we examined peritoneal inflammatory cell patterns to determine their concordance with selected enhanced genes. (biomedcentral.com)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa. (rndsystems.com)
His primary scientific interests are in Receptor, Biochemistry, Molecular biology, Prostaglandin E2 receptor and Cell biology. (research.com)
The scientist's investigation covers issues in Receptor, Biochemistry, Cell biology, Molecular biology and Endocrinology. (research.com)
IL-13 a T-helper type 2 (Th2) cytokine was first defined in 1993 discovered by molecular cloning in turned on human T lymphocytes [1]. (gasyblog.com)

Here, we report the pro-tumorigenic properties of the crosstalk between intestinal tuft cells and type 2 innate lymphoid cells (ILC2) that is evolutionarily optimized for epithelial remodeling in response to helminth infection. (bvsalud.org)
We demonstrate that tuft cell-derived interleukin 25 (IL25) drives ILC2 activation, inducing the release of IL13 and promoting epithelial tuft cell hyperplasia. (bvsalud.org)
Moreover, IL-17A possesses the ability to induce epithelial cells to generate a plethora of inflammatory mediators, actively participating in the orchestration of inflammatory reactions and tissue damage. (betalifesci.com)

Mature human and mouse IL-1 beta share approximately 75% amino acid sequence identity and human IL-1 beta has been found to be active on murine cell lines. (rndsystems.com)
Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1 alpha -stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T1165. (rndsystems.com)

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. (research.com)

Here, we investigated the modulation of γδ T cell-mediated tumor cell lysis by TLR ligands. (aacrjournals.org)
γδ T-cell cytotoxicity and granzyme A/B production were enhanced after pretreatment of tumor cells with TLR3 [poly(I:C)] or TLR7 ligand (imiquimod). (aacrjournals.org)
Poly(I:C) treatment of pancreatic adenocarcinomas followed by coculture with γδ T cells resulted in an upregulation of CD54 on the tumor cells. (aacrjournals.org)
While the resulting tuft cell - ILC2 feed-forward circuit promotes gastric metaplasia and tumor formation, genetic depletion of tuft cells or ILC2s, or therapeutic targeting of IL13 or IL25 alleviates these pathologies in mice. (bvsalud.org)
The Hu08BBz exhibited a 75% reduction in orthotopic tumor growth using low-dose CAR T?cell infusion. (campaignfornonviolentschools.org)
They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)
Vγ9Vδ2 T cells are promising candidates for cellular tumor immunotherapy. (nature.com)
In this respect, the introduction of checkpoint inhibitors to unleash the activity of tumor-reactive T cells has been a milestone in cancer immunotherapy. (nature.com)
It is perhaps a paradox that the peritoneum which is organized to protect the integrity of intraabdominal organs by facilitating infiltration of inflammatory cells to sites of injury and infection, might also serve to facilitate the promotion of tumor growth and spread. (biomedcentral.com)
As EOC advances and penetrates the capsular layer of the ovary, it also carries the potential to expose the peritoneal surface to tumor-cell secreted products. (biomedcentral.com)
Furthermore, a previously developed ADAPT targeting the human epidermal growth factor receptor 2 (HER2) was genetically fused to an improved Horseradish Peroxidase (HRP) variant, thereby combining the idea of tumor-targeted therapy through the ADAPT with the utilization of HRP to enzymatically catalyze the prodrug IAA into its active form. (kth.se)
PM 2.5 increased the mRNA and protein expression of M1 markers including interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and inducible nitric oxide synthase (iNOS) in RAW264.7 cells. (medsci.org)

Interleukin-5 is produced by a number of cell types, and is responsible for the maturation and release of eosinophils in the bone marrow. (biomedcentral.com)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

Distinct tumorigenic potential of abl and raf in B cell neoplasia: abl activates the IL-6 signaling pathway. (sagepub.com)

Mice with C5a receptor-deficient dendritic cells promote induction of Treg and Th17 cells. (medscape.com)
In addition they found that administration of IL-13 conferred an asthma-like phenotype to non-immunized T cell-deficient mice by an IL-4Rα-dependent pathway [3]. (gasyblog.com)
Massive xanthomatosis and atherosclerosis in cholesterol-fed low density lipoprotein receptor-negative mice. (jci.org)
We conclude that LDL receptors are largely responsible for the resistance of wild type mice to atherosclerosis. (jci.org)
Notably, the BsAb-armed T cells did not cause obvious cytokine release syndrome or tissue toxicity in SCID mice bearing human tumors. (biomedcentral.com)

Toll-like receptor (TLR) agonists are considered adjuvants in clinical trials of cancer immunotherapy. (aacrjournals.org)
12] Studies in toll-like receptor (TLR)-mediated signaling in patients with HIES did not reveal anty abnormalities either. (medscape.com)

Diaclone Monoclonal Antibodies are highly specific, high quality mouse monoclonal antibodies for the detection of a broad range of human proteins. (diaclone.com)
Diaclone now offers an extensive selection of monoclonal antibodies produced in cell culture and preservative free. (diaclone.com)
The high titer (1:32000) polyclonal antibodies with high specificity were obtained by immunizing rabbits with the purified recombinant protein. (hindawi.com)
The present review addresses the data that relate interleukin-5 to pulmonary inflammation and function in animal models, and the use of neutralizing anti-interleukin-5 monoclonal antibodies for the treatment of asthma in humans. (biomedcentral.com)
Clinical trials with humanized antibodies against interleukin-5 have begun. (biomedcentral.com)
Furthermore, levels of antibodies to matrilin 1, an extracellular matrix protein predominantly expressed in tracheal cartilage, were significantly higher in patients with relapsing polychondritis, especially in those with respiratory symptoms, than in patients with Wegener granulomatosis , systemic lupus erythematosus , or RA and in healthy controls. (medscape.com)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Human recombinant protein fragment corresponding to amino acids 31-267 of human IL15RA produced in E.coli. (thermofisher.com)
The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli . (hindawi.com)
The rabbits were immunized with the purified recombinant protein. (hindawi.com)

Proportions of CD68+ cells and CD3+ cells that coexpressed MO/MA differentiation factors (CD163, CCR1, CXCR8, VCAM1, and phosphorylated cytosolic phospholipase A 2 [pcPLA 2 ]), which had demonstrated expression in EOC peritoneal samples, were determined by multicolor immunofluorescence. (biomedcentral.com)

It is a single-chain membrane glycoprotein of approximately 200 kd that has 4 allotypic forms on myeloid cells (eg, erythrocytes, granulocytes, monocytes), lymphoid cells (mostly B cells), follicular dendritic cells, and glomerular podocytes. (medscape.com)
Moreover, γδ T cells can directly kill target cells without the involvement of dendritic cells (DCs) and perform dual functional roles in antitumor and anti-infective immunity. (nature.com)

Ad35K-infected cell culture medium, containing recombinant 35K, potently reduced migration of CCR5-transfected 293 cells by 95% in response to the CC-chemokine RANTES, but had no effect on cells transfected with the CX3CR1 fractalkine receptor. (ox.ac.uk)
The chemokine (C-X-C motif) ligand 1 (CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROα, KC, neutrophil-activating protein 3 (NAP-3) and melanoma growth stimulating activity, alpha (MGSA-α). (wikidoc.org)
CXCL8 (IL-8) and the chemokine receptor CCR1 were coexpressed more frequently on MO/MA than on CD3+ cells contrasting with CD68+/CD163+ cells that coexpressed CXCL8 less often. (biomedcentral.com)
PF4 is chemotactic for neutrophils , fibroblasts and monocytes , and interacts with a splice variant of the chemokine receptor CXCR3 , known as CXCR3B. (wikidoc.org)

The complement system, interacting with its regulatory molecules and cellular receptors, plays a central role in the induction and regulation of immunity. (medscape.com)
They are single-chain molecules present on host cellular membranes and belong to the complement control protein family. (medscape.com)
This enables cells to communicate and respond to external environments, maintain cellular homeostasis, and transduce signals. (mdpi.com)
Human dNK cell function is differentially regulated by extrinsic cellular engagement and intrinsic activating receptors in first and second trimester pregnancy. (nih.gov)
Spike glycoprotein has two subunits, S1-responsible for its binding to host cell membrane receptors and S2-responsible for the fusion of viral and host cellular membrane[ 8 , 11 ]. (ijpsonline.com)
Therefore, if procalcitonin enters the circulation, it remains unchanged, with a half-life of around 30 hours, with no evidence that serum procalcitonin binds to cellular receptors of calcitonin or any specific procalcitonin receptors. (medscape.com)

This specificity occurs because only those cells possess the interleukin-5 receptor. (biomedcentral.com)
Small engineered scaffold proteins (ESPs) gain more and more popularity as biological drugs, due to their specificity and applicability in diagnostics and therapy. (kth.se)

CD86 expression is modest in resting B cells, but it rises in response to BCR, CD40, IL-4R, LPS receptor, or 2-adrenergic receptor stimulation. (cusabio.com)
The coding region of IL-33 mature protein was cloned into the prokaryotic expression vector pET-44. (hindawi.com)
Among healthy individuals, the number of CR1 receptors on the red blood cells varies widely, yet the phenotypic expression, regulated by 2 codominant alleles, is stable. (medscape.com)
In humans, interleukin-5 is a very selective cytokine as a result of the restricted expression of the interleukin-5 receptor on eosinophils and basophils. (biomedcentral.com)
After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. (origene.com)
The view that IL-13 is usually pivotal in asthma was further supported by associations with genetic polymorphisms increased expression in disease and the biological effects it exerts on airway inflammatory and structural cells. (gasyblog.com)
Overexpression of miR-146a-3p downregulated the expression of SIRT1 protein in untreated RAW264.7 cells. (medsci.org)
Our data demonstrate that beryllium-stimulated bronchoalveolar lavage cells produce IL-10, and the neutralization of endogenous IL-10 does not increase significantly cytokine production, HLA expression, or T lymphocyte pro- liferation. (cdc.gov)

Furthermore, 35K delivered by intra-peritoneal injection more than halved biogel-induced inflammatory cell recruitment in peritoneal exudates compared to AdGFP medium. (ox.ac.uk)
Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. (rndsystems.com)
A soluble version of the type II receptor is induced by anti-inflammatory agents such as glucocorticoids, IL-4 and IL-13. (rndsystems.com)
IL-33 can promote survival, adhesion, and cytokine production in human mast cells [ 11 ], but the mechanism behind its involvement in inflammatory conditions remains unclear. (hindawi.com)
Primarily synthesized by T cells, particularly CD4+ Th17 cells, IL-17A plays a critical role in the onset and progression of diverse inflammatory responses and autoimmune diseases. (betalifesci.com)
It exerts its influence by fostering the infiltration and activation of inflammatory cells, thereby stimulating essential host defense mechanisms. (betalifesci.com)
RNA-seq results showed that transcription factors and macrophage inflammatory proteins were significantly downregulated in the DNMT3A mutant clones. (frontiersin.org)
The peritoneum and its extension, the intestinal serosa, include a vast surface area for transit of inflammatory cells into the abdominal cavity. (biomedcentral.com)
Specifically T cells mast cells and eosinophils will be the predominant way to obtain IL-13 in asthma using a contribution in the macrophage in COPD [1 6 Various other inflammatory cells and structural cells possess the capacity to create IL-13 Hydralazine hydrochloride in airways disease. (gasyblog.com)
Procalcitonin is also produced by the neuroendocrine cells of the lung and intestine and is released as an acute-phase reactant in response to inflammatory stimuli, especially those of bacterial origin. (medscape.com)

CR1 also regulates complement activation by acting as a cofactor for factor I in the cleavage and degradation of bound C3b and C4b to its inactive forms. (medscape.com)

Structural and functional studies on the human hepatic interleukin-6 receptor. (wikipedia.org)
Among nonhematopoietic cell populations, IL-11, like IL-6 and LIF, can stimulate the synthesis of hepatic acute-phase proteins. (rndsystems.com)

Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. (wikipedia.org)
CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis . (wikidoc.org)
Evidence of a critical part for interleukin-13 in the pathogenesis of asthma Animal models A considerable weight of evidence supporting a role for IL-13 in airways disease is derived from animal models. (gasyblog.com)

May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. (cusabio.com)

The IL-1 receptor type I is a 80 kDa transmembrane protein with demonstrated IL-1 signaling function. (rndsystems.com)
Endocytosis is a fundamental process involved in trafficking of various extracellular and transmembrane molecules from the cell surface to its interior. (mdpi.com)
IL-17R is a transmembrane protein with two fibronectin III-like domains (FN domains) located outside the cell membrane, responsible for mediating ligand-receptor binding. (betalifesci.com)
Contagious human coronavirus (SARS-CoV-2) binds to the receptors of human host cells by transmembrane spike glycoprotein that makes homotrimers protruding from viral surface region[ 9 , 10 ]. (ijpsonline.com)

YAC stands for yeast artificial chromosome, a vector (carrier) created and used in the laboratory to clone pieces of DNA. (definithing.com)

EP(4) prostanoid receptor coupling to a pertussis toxin-sensitive inhibitory G protein. (research.com)

These effects are thought to be at least partially independent of its iron-binding function as apo transferrin has been shown to have growth-promoting effects. (genengnews.com)
It is a recombinant analog of human transferrin that has shown equivalence to serum-derived human transferrin (hTf) and superiority to bovine transferrin (bTf) in stimulating cell growth and productivity for a number of cell lines. (genengnews.com)
Cells were then washed in 10 mL Dulbecco's PBS, spun and resuspended in serum-free, growth factor-free medium. (genengnews.com)
The metabolic dye MTS was added to the remaining media for analysis of viable cell numbers and absorbance was measured from each of the six replicate wells to determine growth. (genengnews.com)
To examine the effect of rTransferrin on CHO cell growth and productivity, two concentrations (1 and 5 µg/mL) of the supplement were tested. (genengnews.com)
5 µg/mL) displayed similar growth and productivity effects in CHO and Sp2/0 cells to iron supplements in common use within the industry, rTransferrin was compared with serum-derived hTf or bTf (each at 5 µg/mL) and three different inorganic iron salts. (genengnews.com)
Human growth hormone and extracellular domain of its receptor: crystal structure of the complex. (sagepub.com)
Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein (PubMed:12441351). (qedbio.com)
It is conceivable that FRs are useful when folate supply is low or when rapid cell growth requires elevated uptake of folate. (biomedcentral.com)

A total of five major Open Reading Frames (ORF) were found in COVID-19: the ORF 1a/b region coding replicase and other enzymes, the spike or S protein ORF, M or membrane glycoprotein ORF, E or small membrane protein ORF and the N or nucleocapsid ORF ( fig. 1 )[ 5 ]. (ijpsonline.com)

Transferrin is an extracellular antioxidant, binding iron so tightly that little unbound iron is available to catalyze the production of free radicals. (genengnews.com)

Note the image below, as well as the article Pediatric Complement Receptor Deficiency may be helpful. (medscape.com)

The IL-33 cDNA sequences encode 270 and 266 amino acid polypeptides for human and mouse, respectively, corresponding to full-length proteins with calculated masses of 30 and 29.9 kDa [ 2 ]. (hindawi.com)
The human IL-11 cDNA encodes a 199 amino acid residue precursor polypeptide with a 21 amino acid residue hydrophobic signal that is processed proteolytically to generate the 178 amino acid residue mature protein. (rndsystems.com)

Proteins from this family, due to their multidirectional activity, participate both in the processes regulating tissues homeostasis, but also play a role in various states of pathology, also in neoplasmic process.The data available do not allow an unequivocal assessment of the role of IL-17 family proteins in the course of neoplasmic disease. (czytelniamedyczna.pl)

Procalcitonin (PCT), a protein that consists of 116 amino acids, is the peptide precursor of calcitonin, a hormone that is synthesized by the parafollicular C cells of the thyroid and involved in calcium homeostasis. (medscape.com)

The sensory apparatus that allows detection of infectious microbes has been deciphered in large part, and it is now known that Toll-like receptors, NOD-like receptors, RIG-I-like helicases, C-type lectin receptors, and cytosolic sensors of DNA, most notably cyclic guanosine monophosphate/adenosine monophosphate synthetase, permit recognition of specific molecules of microbial origin. (mhmedical.com)
Vγ9Vδ2 T cells recognize pyrophosphates secreted by many microbes or overproduced by malignant cells in the context of butyrophilin 3A1 molecules. (nature.com)

Further trials designed to measure eosinophil accumulation and lung function in asthmatic persons are currently underway, and will help to define the role of interleukin-5 and eosinophils in general in this disease. (biomedcentral.com)

Traditionally, transferrin has been supplied in the form of serum-derived human transferrin (hTf), bovine transferrin (bTf), or, more recently, with the move toward serum-free medium (SFM), inorganic iron salts have been used to supply iron to mammalian cells. (genengnews.com)
TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). (qedbio.com)

KLRF1, an activating homodimeric C-type lectin-like receptor (CTLR), is expressed on nearly all natural killer (NK) cells and stimulates their cytoxicity and cytokine release (Kuttruff et al. (nih.gov)

In humans, this protein is encoded by the Cxcl1. (wikidoc.org)
Partial or complete deficiencies of the components of the complement system, including its receptors and regulatory proteins, are now described in humans and may be of a genetic or familial origin or acquired. (medscape.com)
The receptors are widely expressed in humans. (medscape.com)

IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell. (rndsystems.com)
The use of cell lines does, however, introduce a risk the immunoglobulin (Ig)-secreting plasma cell in the spleen, of obtaining cell line-specific features as a result of the trans- gut, or BM [1, 2]. (lu.se)

The pathway uses a novel mechanism in which cytosolic latent transcription factors, known as signal transducers and activators of transcription (STATs), are tyrosine phosphorylated by Janus family tyrosine kinases (Jaks), allowing STAT protein dimerization and nuclear translocation. (sagepub.com)
Binding of IL-13 to the receptor activates the tyrosine kinases Jak 1 Jak 3 and Tyk 2. (gasyblog.com)

Notably, IL-17R recruits Act-1 protein (also known as CIKS) to initiate intracellular signal transduction. (betalifesci.com)
His research integrates issues of Signal transduction and Prostaglandin in his study of Receptor. (research.com)
John W. Regan focuses on Receptor, Cell biology, Prostanoid, Signal transduction and Internal medicine. (research.com)
John W. Regan mostly deals with Receptor, Signal transduction, Prostanoid, Cell biology and Internal medicine. (research.com)

The density of CR1 receptors on cell surfaces varies with the cell type and with the activation of the cell for neutrophils and monocytes. (medscape.com)

Untreated (dashed line histogram) or treated (solid line histogram) peripheral blood mononuclear cells (PBMC) from the BD Phosflow™ T Cell Kit Lyophilized Cells (Catalog No. 560760) were reconstituted in BD Pharmingen™ Stain Buffer (Cat. (bdbiosciences.com)
Peripheral blood mononuclear cells (PBMC) were either left unstimulated (unshaded) or stimulated (shaded) with 100 ng/ml (final concentration) BD Pharmingen™ Recombinant Human IFNγ (Cat. (bdbiosciences.com)

The IL-17A protein, initially known as CTLA-8, was the first discovered member of this family, cloned from rodent-activated hybridoma T cells in 1993. (betalifesci.com)

The vaccinia virus (strain Lister) expresses a 35 kDa soluble protein ('35K') that binds and inactivates a wide range of CC chemokines. (ox.ac.uk)

All experiments performed on Mouse splenocytes treated with Concanavalin A for 24 hrs cells gated on live single cells, in the presence of 10% mouse serum. (bio-rad-antibodies.com)
Matured oocytes had been inseminated with frozen-thawed semen from order MK-0822 a Japanese Dark bull (altered to 2 107 cells/ml) for 5 h at 38.5C within a humidified atmosphere with 5% CO2 in the surroundings, in 1 ml of BO solution containing 10 mg/ml bovine serum albumin and 10 g/ml heparin. (bioskinrevive.com)
From this library, it was possible to achieve protein modules with the ability to simultaneously bind to its intended target as well as to human serum albumin (HSA), a feature that has been shown to increase the binder's half-life in the body. (kth.se)

Anatomy31

Organisms14

Diseases2

Chemicals and Drugs102

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes74

Disciplines and Occupations1

Information Science4

Health Care1

High-affinity12

Cytokines7

Affinity12

Subunit9

Proliferation17

Kinase12

Vitro13

Antigen14

Macrophages10

Inhibition2

Immune22

Gene11

Cytokine receptor4

Antibody9

Effector5

Genes5

Molecular4

Epithelial3

Murine2

Pathways1

Tumor12

Bone marrow2

Signaling pathway1

Mice5

Toll-Like Rec2

Antibodies7

Recombinant protein3

Differentiation1

Dendritic cells2

Chemokine4

Cellular6

Specificity2

Expression10

Inflammatory10

Regulates1

Hepatic2

Pathogenesis3

Immunity1

Transmembrane4

Chromosome1

Inhibitory1

Growth9

Enzymes1

Extracellular1

Deficiency1

CDNA2

Family proteins1

Amino acids1

Molecules2

Eosinophils1

Mammalian2

Lectin1

Humans3

Immunoglobulin2

Tyrosine kinases2

Signal4

Neutrophils1

Peripheral blood2

Hybridoma1

Soluble protein1

Serum3