Protein beta arrestin. Medical search. Frequent questions

It is concluded that PTH stimulates ERK1/2 through several distinct signal transduction pathways: an early G protein-dependent pathway meditated by PKA and PKC and a late pathway independent of G proteins mediated through beta-arrestins. (duke.edu)
It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. (antikoerper-online.de)
Arrestins (abbreviated Arr) are a family of proteins which are important in the regulation of signal transduction G-protein coupled receptors, which at first was discovered, as part of a conserved two step mechanism for regulating the activity of G protein-coupled receptors (GPCR) in rhodopsin visual system by Ursula Kuhn found Scott court, and β-called Global Martin J. Lohse system and kidney and colleagues. (arrestins.com)
Chemokine-binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization. (lsbio.com)

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals, as well as having signalling roles in their own right. (wikipedia.org)
Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. (wikipedia.org)
The valuable source of large-scale genomic information initiated attempts to identify the origin(s) of G protein-coupled receptors (GPCR), count and categorize those genes, and follow their evolutionary history. (springer.com)
Schioth HB, Fredriksson R (2005) The GRAFS classification system of G-protein coupled receptors in comparative perspective. (springer.com)
Sequence comparison does not support an evolutionary link between halobacterial retinal proteins including bacteriorhodopsin and eukaryotic G-protein-coupled receptors. (springer.com)
Violin JD, Lefkowitz RJ (2007) Beta-arrestin-biased ligands at seven-transmembrane receptors. (springer.com)
We are primarily using cytomegaloviruses to examine how the pathogens alter signaling pathways directed by G-protein coupled receptors (GPCRs) to facilitate robust replication in tissues important for host-host dissemination. (uc.edu)
Many types of opioid receptors (e.g., μ , κ , δ ) exist in the nervous system, and they are all typical inhibitory G protein-coupled receptors (GPCRs) [ 30 ]. (hindawi.com)
Here we provide evidence that beta-arrestin, otherwise known to be involved in the regulation of G protein-coupled receptors, serves as an adaptor to bring the oncoprotein E3 ubiquitin ligase MDM2 to the IGF-1R. (duke.edu)
G protein-coupled receptor activation leads to the membrane recruitment and activation of G protein-coupled receptor kinases, which phosphorylate receptors and lead to their inactivation. (duke.edu)
Stimulation of Gi-coupled receptors leads to the activation of mitogen-activated protein kinases (MAP kinases). (duke.edu)
The beta-adrenergic receptor kinase 1 (beta ARK1) is a member of the G protein-coupled receptor kinase (GRK) family that mediates the agonist-dependent phosphorylation and desensitization of G protein-coupled receptors. (duke.edu)
Heart failure is accompanied by severely impaired beta-adrenergic receptor (betaAR) function, which includes loss of betaAR density and functional uncoupling of remaining receptors. (duke.edu)
Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. (antikoerper-online.de)
Virtually all G protein coupled receptors (GPCRs) rapidly undergo desensitization by a common pathway upon activation by ligand binding. (aatbio.com)
Regulatory proteins that down-regulate phosphorylated G-protein membrane receptors, including rod and cone photoreceptors and adrenergic receptors. (lookformedical.com)
A family of serine-threonine kinases that are specific for G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
A subclass of beta-adrenergic receptors (RECEPTORS, ADRENERGIC, BETA). (lookformedical.com)
The adrenergic beta-2 receptors are more sensitive to EPINEPHRINE than to NOREPINEPHRINE and have a high affinity for the agonist TERBUTALINE. (lookformedical.com)
A ubiquitously expressed G-protein-coupled receptor kinase subtype that has specificity for the agonist-occupied form of BETA-ADRENERGIC RECEPTORS and a variety of other G-PROTEIN-COUPLED RECEPTORS. (lookformedical.com)
A family of G-protein-coupled receptors that was originally identified by its ability to bind N-formyl peptides such as N-FORMYLMETHIONINE LEUCYL-PHENYLALANINE. (lookformedical.com)
Since LPA1 receptors, like all G protein-couple receptors, cannot be easily visualized by an antibody, it is hard to discern the actual localization of the protein. (molcells.org)
Protease-activated receptors (PARs) and the neurokinin 1 receptor (NK1R) belong to the G protein-coupled receptor (GPCR) family. (scientificarchives.com)
G protein-coupled receptors (GPCRs) are a large family of cell membrane receptors. (scientificarchives.com)
Phosphatidic acid phospholipase A1 mediates ER-Golgi transit of a family of G protein-coupled receptors. (academictree.org)
Calcium/calmodulin-dependent serine protein kinase (CASK) is a new intracellular modulator of P2X3 receptors. (shengsci.com)

and protein kinase activity. (nih.gov)
Orthologous to human MAPK1 (mitogen-activated protein kinase 1). (nih.gov)
Transient activation of AMP-activated protein kinase at G1/S phase transition is required for control of S phase in NIH3T3 cells. (nih.gov)
A beta-adrenergic receptor kinase-like enzyme is involved in olfactory signal termination. (duke.edu)
We have previously shown that second-messenger-dependent kinases (cAMP-dependent kinase, protein kinase C) in the olfactory system are essential in terminating second-messenger signaling in response to odorants. (duke.edu)
Bbeta-adrenergic receptor kinase-1 levels in catecholamine-induced myocardial hypertrophy: regulation by beta- but not alpha1-adrenergic stimulation. (duke.edu)
beta2-Adrenergic receptor regulation by GIT1, a G protein-coupled receptor kinase-associated ADP ribosylation factor GTPase-activating protein. (duke.edu)
Chronic human heart failure is characterized by abnormalities in beta-adrenergic receptor (betaAR) signaling, including increased levels of betaAR kinase 1 (betaARK1), which seems critical to the pathogenesis of the disease. (duke.edu)
Direct evidence that Gi-coupled receptor stimulation of mitogen-activated protein kinase is mediated by G beta gamma activation of p21ras. (duke.edu)
Essential role of beta-adrenergic receptor kinase 1 in cardiac development and function. (duke.edu)
Expression of a beta-adrenergic receptor kinase 1 inhibitor prevents the development of myocardial failure in gene-targeted mice. (duke.edu)
Functional desensitization of the isolated beta-adrenergic receptor by the beta-adrenergic receptor kinase: potential role of an analog of the retinal protein arrestin (48-kDa protein). (duke.edu)
The beta-adrenergic receptor kinase is an enzyme, possibly analogous to rhodopsin kinase, that multiply phosphorylates the beta-adrenergic receptor only when it is occupied by stimulatory agonists. (duke.edu)
Functionally active targeting domain of the beta-adrenergic receptor kinase: an inhibitor of G beta gamma-mediated stimulation of type II adenylyl cyclase. (duke.edu)
The beta-adrenergic receptor kinase (beta ARK) phosphorylates its membrane-associated receptor substrates, such as the beta-adrenergic receptor, triggering events leading to receptor desensitization. (duke.edu)
We use a number of model systems (pituitary cells, cell lines, transgenic animals) to study signal pathways for a hypothalamic peptide hormone, TRH, which acts via a G protein coupled receptor to increase cytoplasmic calcium and protein kinase C activity. (rochester.edu)
Paroxetine is a direct inhibitor of g protein-coupled receptor kinase 2 and increases myocardial contractility. (rochester.edu)
Although it is highly homologous to G-PROTEIN-COUPLED RECEPTOR KINASE 2, it is not considered to play an essential role in regulating myocardial contractile response. (lookformedical.com)
A G-protein-coupled receptor kinase subtype that is primarily expressed in the MYOCARDIUM and may play a role in the regulation of cardiac functions. (lookformedical.com)
A protein kinase C activator, 4 beta-phorbol 12 beta-myristate 13 alpha-acetate, had opposite effects on PGE1-mediated IP release and cyclic GMP formation (inhibitory) and cyclic AMP formation (stimulatory). (shengsci.com)
The present study investigated the role of calcium/calmodulin-dependent serine protein kinase (CASK) in interacti. (shengsci.com)

Oesterhelt D (1998) The structure and mechanism of the family of retinal proteins from halophilic archaea. (springer.com)
A 48-Kd protein of the outer segment of the retinal rods and a component of the phototransduction cascade. (lookformedical.com)
The delivery of proteins to the presynaptic terminals of guinea pig retinal ganglion cells by two of the major components of axonal transport, and the subsequent persistence and turnover of those proteins were examined in this study. (shengsci.com)

Say you have a G-protein coupled receptor (GPCR) of unknown function-with this assay you can determine whether it effects a stimulatory or inhibitory response, or induces necrosis under your experimental conditions, Dr. Jansson proposed as an example. (genengnews.com)
The binding of beta-arrestin, a cytoplasmic protein, to an activated receptor deactivates the GPCR signaling and initiates the translocation of the receptor into the cell where the ligand is removed, and the receptor is recycled back to the cell membrane. (aatbio.com)
Since desensitization only occurs with an activated receptor, monitoring beta-arrestin translocation and subsequent receptor recycling provides a reliable method to detect the activation of a GPCR target. (aatbio.com)
Cell Meter™ Beta-Arrestin translocation GPCR Signaling Kit provides a powerful functional assay to screen activities of target compounds against known or orphan GPCR targets via fluorescence imaging. (aatbio.com)
Mix 3 µg of DNA [for example, 1.5 µg of Beta-arrestin-GFP DNA stock solution and 1.5 µg DNA of the GPCR that you are interested] with 200 µL of serum-free medium. (aatbio.com)
This image set is of Transfluor assay where an orphan GPCR (G-protein coupled receptor) is stably integrated into a beta-arrestin GFP (green) expressing U2OS cell line also showing nucleus (red). (cellimagelibrary.org)

The protein encoded by this gene is an orphan G protein-coupled receptor whose ligand is unknown. (nih.gov)

Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (arrestins.com)

The purpose of the present study was to identify the contributions of distinct signaling mechanisms to PTH-stimulated activation of the mitogen-activated protein kinases (MAPK) ERK1/2. (duke.edu)

Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA038474 (shown in green). (cellimagelibrary.org)

Here we find that in Caenorhabditis elegans, mouse, rat and human neurons, dendritically and axonally polarized transmembrane proteins are recognized by endocytic machinery in the AIS, robustly endocytosed and targeted to late endosomes for degradation. (stanford.edu)
RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. (antikoerper-online.de)

Considering the signaling pathway of TGFβ1 (an inhibitor of iNOS mRNA expression), silencing of RNA for TAK-1 upregulates membrane-bound fractalkine tripartite motif 28 (TRIM28)/transcriptional intermediary factor 1β (TIF1β) functions as an E3 ubiquitin ligase and specificity protein 1 negatively regulate TGFβ1 levels to upregulate the generation of peroxynitrite, leading to increased shedding of membrane-bound fractalkine via SP/NK1R signaling. (scientificarchives.com)
Accumulation of misfolded proteins and alterations in the ubiquitin-proteasome pathway are associated with various neurodegenerative conditions of the CNS and PNS. (shengsci.com)

Fujisawa Y, Kato H, Iwasaki Y (2001) Structure and function of heterotrimeric G proteins in plants. (springer.com)
They share a common structure and signal through HETEROTRIMERIC G-PROTEINS. (lookformedical.com)

This later phase of ERK1/2 activation at 30-60 min was blocked by depletion of cellular beta-arrestin 2 and beta-arrestin 1 by small interfering RNA. (duke.edu)
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport. (lookformedical.com)

My work on the role of CXC-Chemokines that have pleiotropic functions in cancer progression and response to therapy have elucidated the potential role of scaffold proteins (e.g., beta-arrestins) in cancer progression and response to treatment. (augusta.edu)

Scholars@Duke publication: Distinct beta-arrestin- and G protein-dependent pathways for parathyroid hormone receptor-stimulated ERK1/2 activation. (duke.edu)
Scholars@Duke publication: {beta}-Arrestin is crucial for ubiquitination and down-regulation of the insulin-like growth factor-1 receptor by acting as adaptor for the MDM2 E3 ligase. (duke.edu)

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (arrestins.com)

In the present study, we demonstrated that the synthetic GPR52 agonist, 3-BTBZ, equipotently induces cAMP accumulation, ERK1/2 phosphorylation, and β-arrestin-1 and -2 recruitment in transfected HEK293T cells. (monash.edu)
Furthermore, knock down of β-arrestin-2 in frontal cortical neurons abolished 3-BTBZ-induced ERK1/2 phosphorylation, but not cAMP accumulation. (monash.edu)

Probable beta-arrestin(arr-1) is a recombinant protein expressed in E. coli. (arrestins.com)
All of our recombinant proteins are manufactured in strictly controlled facilities and by using a well established technology which guarantees full batch-to-bact consistency and experiment reproducibility. (arrestins.com)
Note The recombinant protein can start to be detected as early as 16 hours after transfection. (aatbio.com)
Full-length recombinant human caspase-3 protein was used as immunogen. (thermofisher.com)
Recombinant Human beta-Arrestin 1 is produced by our E.coli expression system and the target gene encoding Met1-Arg418 is expressed with a 6His tag at the C-terminus. (arrestins.com)

The beta-arrestin isoform 1 appeared to be more strongly associated with the receptor than isoform 2, and in a molecular context it was 4-fold more efficient in inducing polyubiquitination of IGF-1R, a reaction that required the presence of beta-arrestin and MDM2. (duke.edu)
To our knowledge this is the first study demonstrating a defined molecular role of beta-arrestin with direct relevance to cell growth and cancer. (duke.edu)
Several isoforms of the protein with molecular sizes of 47 kDa and 52 kDa exist due to multiple ALTERNATIVE SPLICING. (lookformedical.com)

Pertussis toxin B-pentamer mediates intercellular transfer of membrane proteins and lipids. (uc.edu)
Those factors may include receptor species orthologs, receptor dimerization, receptor density, gylcosylation, the presence of specific G-protein heterotrimers, RGS-proteins and arrestins, compartmentation of signalling proteins and lipid composition of the membrane, to name a few factors. (aspetjournals.org)

Monitor the beta-arrestin translocation induced by the receptor activation under a fluorescence microscope with the FITC filter (Ex/Em = 488/530 nm). (aatbio.com)

Conversely, in the two studied human melanoma cell lines both beta-arrestin isoforms seemed to be involved in IGF-1R ubiquitination. (duke.edu)

Beta-arrestin-2, also known as arrestin beta-2, is an intracellular protein that in humans is encoded by the ARRB2 gene. (wikipedia.org)
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. (lookformedical.com)

Furthermore, stimulation of hPTH1R with PTH analogues, [Trp1]PTHrp-(1-36) and [d-Trp12,Tyr34]PTH-(7-34), selectively activated G(s)/PKA-mediated ERK1/2 activation or G protein-independent/beta-arrestin-dependent ERK1/2 activation, respectively. (duke.edu)
These results suggest a β-arrestin-2-dependent mechanism for GPR52-mediated ERK1/2 signaling, which may link to cognitive function in vivo. (monash.edu)

However, because depletion of beta-arrestin 1 almost completely eliminated degradation, and IGF-1 induced down-regulation of the receptor in these cells, whereas beta-arrestin 2 only had a partial effect, beta-arrestin 1 seems to the more important isoform in affecting the expression of IGF-1R. (duke.edu)

This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc. (arrestins.com)

Clathrin also interacts with cytoskeletal proteins. (lookformedical.com)

Abcam: antibodies, proteins, kits. (abcam.com)

Cardiac beta ARK1 inhibition prolongs survival and augments beta blocker therapy in a mouse model of severe heart failure. (duke.edu)

Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. (wikipedia.org)

Gavin BA , Dolph MJ, Deleault NR, Geoghegan JC , Khurana V , Feany MB , Dolph PJ , Supattapone S . Accelerated accumulation of misfolded prion protein and spongiform degeneration in a Drosophila model of Gerstmann-StrÃ¤ussler-Scheinker syndrome. (academictree.org)

For example, we can 'tag' signaling proteins with green fluorescent protein (GFP) and then follow their movements in the cell during hormonal stimulation in real time. (rochester.edu)

Neurons are highly polarized cells that face the fundamental challenge of compartmentalizing a vast and diverse repertoire of proteins in order to function properly1. (stanford.edu)

The LIN-14 transcription factor likely directly represses dma-1 gene expression through a transcriptional means, whereas the LIN-41 tripartite motif protein likely indirectly promotes dma-1 gene expression through a posttranscriptional means. (stanford.edu)

Whereas mitophagy mediated through an Atg7 (autophagy related 7)-dependent mechanism serves as an essential mechanism to maintain mitochondrial quality during the initial development of obesity cardiomyopathy, Rab9 (Ras-related protein Rab-9A)-dependent alternative mitophagy takes over the role during the chronic phase. (bvsalud.org)

In contrast, loss-of-function mutations in the let-7 microRNA gene prevent a developmental decline in dendritic growth ability, whereas loss-of-function mutations in its direct target, the lin-41 tripartite motif protein gene, cause further decline. (stanford.edu)

Eye proteins are the biological molecules that make up the various structures of the eye and are essential for its proper function. (lookformedical.com)

It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. (thermofisher.com)
In summary, the overexpression of LPA 1 did not affect neuronal migration, and the protein expression of LPA 1 was mainly located in the ventricular zone and cortical plate within the developing mouse cerebral cortex. (molcells.org)
Neuronal polarity requires sorting and maintaining a unique set of proteins to the neuron's distinct axonal and somatodendritic domains. (stanford.edu)

Aggregates containing ubiquitin and peripheral myelin protein 22 (PMP22) have been observed in the Trembler J mouse model of Charcot-Marie-Tooth disease ty. (shengsci.com)

The protein may interact with the agonist DOI in 5-HT2A receptor signaling. (wikipedia.org)
Kim JY, Haastert PV, Devreotes PN (1996) Social senses: G-protein-coupled receptor signaling pathways in Dictyostelium discoideum . (springer.com)
G-protein-coupled receptor signaling and neural tube closure defects. (nih.gov)
In addition, we use biochemical and genetic approaches to identify proteins that interact with the receptor during biosynthesis, signaling and desensitization. (rochester.edu)
The Role of Membraneembedded DUOX2 on Ectodomain Shedding via G protein-coupled Receptor Signaling. (scientificarchives.com)

Arrestin quenches G-protein activation by binding to phosphorylated photolyzed rhodopsin. (lookformedical.com)

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. (antikoerper-online.de)
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. (thermofisher.com)
The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). (antikoerper-online.de)

Arrestin beta 2 is crucial for the development of tolerance to morphine and other opioids. (wikipedia.org)
In this way, beta-arrestin acts as a crucial component in the ubiquitination and down-regulation of the receptor. (duke.edu)

False positive non-synonymous polymorphisms of G-protein coupled receptor genes. (nih.gov)

Anatomy7

Organisms3

Chemicals and Drugs33

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Psychiatry and Psychology1

Phenomena and Processes17

Information Science3

Signal transduction4

Receptors26

Kinase21

Retinal3

GPCR6

Ligand1

Freeze-thaw cycles1

Kinases1

Antibody1

Transmembrane proteins2

Pathway2

Heterotrimeric2

Cellular2

Scaffold1

Scholars@Duke2

CDNA1

Phosphorylation2

Recombinant5

Molecular3

Membrane2

Translocation1

Isoforms1

Intracellular2

ERK12

Depletion1

ELISA1

Clathrin1

Antibodies1

Cardiac1

Adrenergic1

Accumulation1

Stimulation1

Neurons1

Gene Expression1

Mechanism1

Tripartite Motif1

Molecules1

Neuronal3

Ubiquitin1

Signaling5

Rhodopsin1

Family3

Crucial2

Genes1