Protein being assayed. Medical search. Frequent questions

Upon simultaneous binding of two PLA probe-coupled antibodies to two cognate protein interaction partners, the PLA probes are brought into proximity. (medscape.com)
The CosMx Protein Assays rely on antibodies coupled to unique oligonucleotide tags designed for an imaging-based readout on the CosMx™ Spatial Molecular Imager (SMI). (nanostring.com)
For this research, recombinant protein at high levels of purity, as well as information on catalytic mechanisms guided by crystal structures and selective antibodies are vital. (jove.com)
Many assays in basic and clinical science research rely exclusively on antibodies. (nih.gov)
The rigor and reproducibility of many assays commonly used by researchers in the obesity research field could be substantially improved by applying Mass Spectrometry (MS) instead of relying only on antibodies. (nih.gov)
The use of highly reproducible antibodies, such as the recombinant monoclonal antibodies, only for the enrichment of the proteins and peptides of interest in combination with MS assays for developing targeted Immuno-MRM assays, is also an option. (nih.gov)
CALIXAR's approach allows to preserve the original structure and function of membrane proteins (GPCRs, Ion Channels, Transporters, Receptors, Anchors and Viral Proteins) providing solutions for pharmaceutical industries, biotechnology companies and academic teams to develop conformational antibodies, formulate new vaccines, carry out Structure Based Drug Discovery and/or HTS assays. (calixar.com)
Cloud-Clone, a company headquartered in Houston USA with a facility located in Wuhan China, produces ELISA kits, proteins, and antibodies. (caltagmedsystems.co.uk)
This assay uses readily-available reagents and straightforward techniques that avoid the difficulty of purifying proteins or generating antibodies required for other binding studies. (nih.gov)
Antibodies to the HIV-1 major group-specific antigen (GAG) protein p24, and its precursor p55, are the earliest detected after infection by Western blot and tend to decrease or become undetectable with onset or progression of clinical symptoms (4-9). (cdc.gov)
In contrast, antibodies to the envelope (ENV) precursor protein gp160 and the final ENV proteins (gp120 and gp41) can be detected in specimens from virtually all HIV-infected persons regardless of clinical stage (4-9). (cdc.gov)
Although the overall sensitivity and specificity of the Western blot for detection of antibodies to the various viral proteins are high, there has been substantial debate regarding the interpretive criteria. (cdc.gov)
Labeled myristoylated proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources, or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis. (transcriptionfactor.org)
An Enzyme-Linked Immunosorbant Assay (ELISA) for the semi-quantitative detection of IgA antibodies to tissue transglutaminase (endomysium) in human serum. (cdc.gov)

The Advanced Protein Assays are powerful reagents in that they are very tolerant to many laboratory chemicals that can often interefere with other types of protein assays. (cytoskeleton.com)
Included in the kit are TGR5 Reporter Cells, two optimized media for use in recovering the cryopreserved cells and for diluting test samples respectively, the reference activator Lithocholic Acid (LCA), Luciferase Detection Reagents, and a cell culture-ready assay plate. (indigobiosciences.com)
Bulk volumes of TGR5 assay reagents are available to accommodate high throughput screening applications. (indigobiosciences.com)
BD Biosciences offers a complete suite of assays and reagents for visualizing key cellular events. (bdbiosciences.com)
Choose from a variety of BD Biosciences assays and reagents for visualizing cellular events such as apoptosis, cell proliferation and the cell cycle. (bdbiosciences.com)
The proposed assay protocols must employ reagents and readouts that can be used in the HTS environment. (nih.gov)
Human IgG antibody Laboratories manufactures the protein assay reagent a #5000113 reagents distributed by Genprice. (fgf-erk.com)
The kit contains sufficient reagents for the evaluation of 960 assays in ten 96-well plates or 192 assays in eight 24-well plates. (fgf-erk.com)

This allows the conversion of an antibody-based detection of proteins into an oligonucleotide-based detection system, thus amenable to amplification, to generate a signal that can be detected and quantified by fluorescence microscopy. (medscape.com)
This creates a high local concentration of a given fluorophore - corresponding to the presence and localization of the target protein complex - which can be visualized and quantified by fluorescence microscopy. (medscape.com)
Andromeda measures the thermal unfolding of a protein by detecting changes in fluorescence during a thermal ramp. (calixar.com)
In vitro, R669C was significantly more thermally stable than the wild type protein as judged by a fluorescence assay. (manchester.ac.uk)
To this end, a fluorescence-based thermal stability readout was investigated as a drug binding assay to screen potential allocrites of wild type human P â glycoprotein. (manchester.ac.uk)
Since the myosin fusion protein and its partner are labeled with different fluorescent tags, an unambiguous fluorescence overlap will be visible as discrete points along the periphery of the cell. (nih.gov)

Appropriate assays might include but are not limited to: o Biochemical or cell-based assays of activity or interaction involving proteins and/or other biological molecules. (nih.gov)

When SDS concentrations are below critical micelle concentration (known as CMC, 0.00333%W/V to 0.0667%) in a Coomassie dye solution, the detergent tends to bind strongly with the protein, inhibiting the protein binding sites for the dye reagent. (wikipedia.org)
By using the Bradford protein assay, one can avoid all of these complications by simply mixing the protein samples with the Coomassie brilliant blue G-250 dye (Bradford reagent) and measuring their absorbances at 595 nm, which is in the visible range and may be accurately measured by the use of a mobile smartphone camera. (wikipedia.org)
To accomplish this, they incubate the antiserum with a color-sensitive reagent that changes from green to purple in the presence of protein. (nih.gov)
This single reagent can be used for the quantitative determination of total protein in urine (proteinuria) and cerebrospinal fluid (blood-brain barrier). (bioassaysys.com)
The Protein Assay Reagent A #5000113 reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (fgf-erk.com)
CytoSelect Cell Proliferation Assay Reagent (Fluorometric) provides a fluorometric format for measuring and monitoring cell proliferation. (fgf-erk.com)

Expression and purification of fumarylacetoacetate hydrolase domain-containing proteins is described with examples (expression in E. coli , FPLC). (jove.com)
This article presents a series of consecutive methods for the expression and purification of FAHD proteins, mainly FAHD protein 1 (FAHD1) orthologues among species (human, mouse, nematodes, plants, etc. (jove.com)
CALIXAR is unique on the market and at the vanguard of Membrane protein extraction/solubilization, purification and characterization services. (calixar.com)
Expression And Purification Of The Multidrug Resistance Protein P â Glycoprotein And High â Throughput Assay Development For Drug Discovery. (manchester.ac.uk)
TaDa permits genome-wide profiling of DNA- or chromatin-binding proteins without cell sorting, fixation, or affinity purification. (nih.gov)
To improve the yield of these high-sensitivity forms of RBD and support the increased demand for this antigen in serology assays, we investigated several protein expression variables including DNA elements such as promoters and signal peptides, cell culture expression parameters, and purification processes. (nih.gov)
Potential Applications crystallography researching infectious diseases researching signal transduction drug and vaccine development medical diagnostics Benefits and Advantages allows facile manipulation of membrane and other insoluble protein simplified purification of insoluble proteins permits using insoluble proteins in microarrays, microtiter plates, etc. (elsevierpure.com)

To utilize the GST-S protein as a useful biomarker for environmental contamination, we developed a polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) in the intertidal copepod Tigriopus japonicus. (nih.gov)
Purified proteins are used for crystallization and antibody production and employed for enzyme assays. (jove.com)
Proteins of similar or lower quality may be employed in enzyme assays or used as antigens in detection systems (Western-Blot, ELISA). (jove.com)
In bacteria, the monopolar localization of enzymes and protein complexes can result in a bimodal distribution of enzyme activity between the dividing cells and heterogeneity of cellular behaviors. (nature.com)
This results in a particle suspension that can be used in any assay requiring fluidity, such as in enzyme assays, microtiter plate screens, micro-array probings, or injections. (elsevierpure.com)

Background We aimed to assess the specificity of SARS-CoV-2 antibody detection assays among people with known tissue-borne parasitic infections. (medrxiv.org)
Results The specificity of assays was 95-98% overall. (medrxiv.org)

For several analytes and peptide/protein hormones, mass spectrometric assays that are more specific and less variable than previous measurements have been already developed, and some are already commonly used in high-throughput clinical chemistry laboratories. (nih.gov)
HIGH THROUGHPUT MOLECULAR SCREENING ASSAY DEVELOPMENT RELEASE DATE: January 20, 2004 RFA Number: RFA-RM-04-012 (Reissued for FY2005, see RFA-RM-05-011 ) Department of Health and Human Services (DHHS) PARTICIPATING ORGANIZATION: National Institutes of Health (NIH) (http://www.nih.gov) This RFA is developed as an NIH roadmap initiative (http://nihroadmap.nih.gov). (nih.gov)
There are a number of characteristics that make an assay suitable for high throughput approaches. (nih.gov)
Proposals should include assay development plans that are sufficient to demonstrate reproducibility in a low-to-moderate throughput setting, i.e., tens or hundreds of compounds, and must be feasible for adaptation to an automated, high-throughput screening approach. (nih.gov)

Multiplexed targeted mass spectrometry assays for prostate cancer-associated urinary proteins. (nih.gov)
Herein, we report the development of sensitive targeted mass spectrometry assays for simultaneous quantification of 10 prostate cancer-associated proteins in urine. (nih.gov)
We have adapted and streamlined mass spectrometry based functional assays, described by others, to develop a rapid, robust, broad-band and fieldable protocol with the primary goal of RIP toxin detection in unknown powder materials. (listlabs.com)
Complementary to intact protein detection and proteomics methods, this protocol involves monitoring the depurination of selected DNA substrates by mass spectrometry. (listlabs.com)

For assay validation, the reproducibility was assessed and demonstrated no significant difference between intra- and inter-experimental variation (n = 3). (medscape.com)
A study by Morimont et al indicated that the interlaboratory reproducibility and intralaboratory precision are excellent for the endogenous thrombin potential (ETP)-based APCR assay, an assay recommended for use in contraceptive agent development. (medscape.com)
Several studies reported higher sensitivity and reproducibility of FCA versus 51 chromium release assay [ 6 , 12 , 13 , 15 ]. (hindawi.com)

The DC (detergent compatible) protein assay is a colorimetric assay for protein concentration following detergent solubilization. (genmallestore.com)

Several diseases are caused by aggregated proteins, in particular neurodegenerative diseases such as Alzheimer's (and other Tauopathies like FTD), Parkinson's, Huntington's, ALS, and many others, which still have a paucity of treatments. (selvita.com)
Typically, proteins are related to neurodegenerative diseases as Parkinson's (e.g. alpha-synuclein), Alzheimer's disease (e.g. beta-amyloid) and others. (selvita.com)
It causes oxidative damage to proteins, lipids and nucleic acids, which can further lead to the development of cancer, asthma, ischemia, diabetes, and cardiovascular and neurodegenerative diseases. (researchandmarkets.com)

Multiple Reaction Monitoring) for proteins and peptides of primary interest to the obesity research community (e.g. (nih.gov)
Binding screening studies allowed Selvita to develop the SAR of compounds able to bind specifically to these proteins (or peptides) and to be used, as example, as a warhead for PROTACs. (selvita.com)

BioAssay Systems Total Protein Assay Kit is based on an improved pyrogallol red-molybdate protein dye-binding assay. (bioassaysys.com)
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you. (bioassaysys.com)

It is a quick and accurate spectroscopic analytical procedure used to measure the concentration of protein in a solution. (wikipedia.org)
The increase of absorbance at 595 nm is proportional to the amount of bound dye, and thus to the amount (concentration) of protein present in the sample. (wikipedia.org)
While other detergents interfere with the assay at high concentration, the interference caused by SDS is of two different modes, and each occurs at a different concentration. (wikipedia.org)
This can cause underestimations of protein concentration in solution. (wikipedia.org)
A high concentration of buffer will cause an overestimated protein concentration due to depletion of free protons from the solution by conjugate base from the buffer. (wikipedia.org)
This will not be a problem if a low concentration of protein (subsequently the buffer) is used. (wikipedia.org)
Additionally, the absorption maxima at 280 nm requires that proteins contain aromatic amino acids such as tyrosine (Y), phenylalanine (F) and/or tryptophan (W). Not all proteins contain these amino acids, a fact which will skew the concentration measurements. (wikipedia.org)
Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration. (activemotif.com)
After exposure to 4 metals (Ag, As, Cd, and Cu) to T. japonicus, the amount of TJ-GST-S protein was significantly elevated in a concentration-dependent manner. (nih.gov)
This allows them to offer a custom protein expression and peptide synthesis service, which can be catered to individual requirements, including lead time, yield, concentration, purity, modification. (caltagmedsystems.co.uk)
Total protein concentration can be determined by several methods. (bioassaysys.com)
The color intensity at 600nm is directly proportional to the total protein concentration in the sample. (bioassaysys.com)

The measles virus sandwich-capture IgM antibody assay, offered through many local health departments and through the CDC, is the quickest method of confirming acute measles. (medscape.com)

The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a commonly used antigen for serology assays critical to determining the extent of SARS-CoV-2 exposure in the population. (nih.gov)
However, in a recent study, the protein with a mobility of 160 kilodaltons (kd) present in commercially available Western blots and in viral lysate antigen preparations was identified as a multimer of the gp41 protein (10,11). (cdc.gov)

Also a recombinant TJ-GST-S protein was purified as a standard for ELISA development. (nih.gov)

This imaging-based readout yields a series of fluorescent protein images to provide relative protein abundance at single cell and subcellular resolution. (nanostring.com)
Here we demonstrated that constitutively expressed fluorescent proteins can be used to track the growth of individual strains in different liquid media. (biorxiv.org)
Furthermore, we were able to track the growth of two different strains simultaneously by using fluorescent proteins with differential excitation and emission wavelengths. (biorxiv.org)

The Western blot assay is a method in which individual proteins of an HIV-1 lysate are separated according to size by polyacrylamide gel electrophoresis. (cdc.gov)

Unlike conventional approaches that would alter the native structure of membrane proteins, CALIXAR's approach allows the conservation of the functional and structural integrities of the targets/ antigens. (calixar.com)
INDIGO's TGR5 assay kit is all-inclusive, offering everything needed to perform the assay to quantify any functional activities, either activating or inhibitory, that a test compound may exert against TGR5. (indigobiosciences.com)

CALIXAR proposes Thermal Stability Assays to get information about expression levels, stability and ligand binding of membrane proteins. (calixar.com)
CALIXAR offers the access to cutting-edge technologies and to characterize ligand interaction with any membrane protein targets. (calixar.com)
With the first Monolith X (Nanotemper) installed in France, CALIXAR increases its analytical capabilities in ligand binding to any type of membrane protein. (calixar.com)
Thanks to the MicroScale Thermophoresis (MST) combined with isothermal spectral shift detection, CALIXAR offers to determine interactions of membrane proteins with unlabeled ligands in solution, without the need for immobilization. (calixar.com)
CALIXAR offers ligand binding characterization to any membrane protein using radiolabeled [3H]-ligand. (calixar.com)

Atellica CH Total Protein II- In vitro diagnostic use in the quantitative determination of total protein in human serum and plasma (lithium heparin) using the Atellica CH Analyzer. (fda.gov)
For quantitative determination of total protein. (bioassaysys.com)

In the kinesin assay microtubules are present to stimulate ATPase, whereas for myosin the S1 domain is generated by proteolytic cleavage and this domain is used either directly on F-actin, or using a reconstituted thin filament / sarcomere assay. (cytoskeleton.com)

The well-established and robust radioactive method enables high-sensitive detection of membrane protein / ligand interactions. (calixar.com)
More work needs to be done to make this assay robust for a high â throughput format. (manchester.ac.uk)
The assay must be robust, reproducible and have a readout that is amenable to automated analysis. (nih.gov)
BioVision offers EZClick™ Myristoylated Protein Assay Kit, a highly specific, simple and robust method for labeling and detection of myristoylated proteins. (transcriptionfactor.org)

After reconstitution into porcine brain polar lipids, purified R669C basal ATPase activity was significantly higher than that of the reconstituted wild type protein, although the activity could not be significantly stimulated by a well-characterised allocrite (verapamil). (manchester.ac.uk)
1. CenPE and Eg5 kinesin motor IC50 determinations using microtubule stimulated ATPase assays, with monthly analysis of 10 to 30 compounds, results turnaround within one week. (cytoskeleton.com)
2. Calcium activated sarcomeric myosin S1 ATPase assays with tropomyosin/troponin coated F-actin (thin filaments) as substrate. (cytoskeleton.com)

This was set up in a medium â throughput fashion and was found to be a good assay to determine if drugs could interact with wild type human P â glycoprotein, although it could not be used to differentiate between substrates and inhibitors. (manchester.ac.uk)

Here, we present a multi-omics approach allowing the simultaneous targeted quantification of mRNA and protein expression in single cells and investigate its performance to dissect the heterogeneity of human immune cell populations. (nanostring.com)

The proposed assays should be highly reproducible, easily transferable to other laboratories, easy to multiplex, and validated in human plasma or serum. (nih.gov)
INDIGO's assays have been demonstrated to provide fast, accurate, reproducible results, and we are dedicated to supporting scientists through reliable science, easy-to-use products, and a highly qualified technical support team. (indigobiosciences.com)

CosMx Protein Assays provide unbiased spatial proteomic analysis of tissue sections to expand the number of markers you can profile compared to traditional immunohistochemical and immunofluorescent methods. (nanostring.com)

Our ligand binding assays services help scientists and researchers who want to obtain accurate ligand binding parameters, compare several ligands in term of binding properties or just identify a specific interaction of their ligands with the membrane target of interest. (calixar.com)
A2AR ligand binding assays using microscale thermophoresis (MST) combined with isothermal spectral shift. (calixar.com)
Ligand binding assays were performed on full-length wild-type A2A adenosine receptor either in cell membrane fractions ( A ) or purified in solution ( B ) and with CGS21680 agonist or ZM241385 inverse agonist using a Monolith X (Nanotemper) combining microscale thermophoresis with isothermal spectral shift detection. (calixar.com)
The VENTANA PD-L1 (SP263) Assay is a test done in a laboratory using samples from non-small cell lung cancer (NSCLC) patients which doctors use to see if patients have programmed cell death ligand (PD-L1) in their cancer. (fda.gov)

To eliminate systematic errors from possible contamination with serum proteins and low-density lipoprotein (LDL) or very-LDL (VLDL) in BDS-CEC, the deviation of each CEC-BDS from the regression equation was compared with serum protein, LDL, and triglyceride (TG) levels. (portlandpress.com)

A protein fused to a fragment of myosin X will carry its binding partners to the cell periphery. (nih.gov)
Available for licensing are a variety of cDNAs which may be used for generating fluorescently-tagged myosin X fusion proteins, for use in the assay described above. (nih.gov)
Also available are a number of constructs incorporating other fluorescently-tagged myosins, kinesins, myosin and kinesin binding partners and a variety of PDZ scaffold proteins. (nih.gov)

Here, we describe a method to detect microcystins in human plasma by using immunocapture followed by a protein phosphatase inhibition assay. (nih.gov)

We assessed a colloidal gold immunochromatographic assay (GICA) for rapid detection of influenza A (H7N9) and compared it with reverse-transcription-polymerase chain reaction (RT-PCR) and viral culture. (hindawi.com)
Then we compared the results of the GICA with viral culture and the RT-PCR assay. (hindawi.com)
We found that the GICA specifically reacted with recombinant HA protein, virus lysates, and clinical samples from H7 subtype viruses and was more sensitive than viral culture but less sensitive than RT-PCR. (hindawi.com)

The Molecular probes handbook is an excellent assay review resource as well as a plug for their own products. (bio.net)
Recombinant SUV39H1 (82-412) protein corresponding to amino acids 82-412 of SUV39H1 protein (accession number NP_003164.1) was expressed in E. coli cells and contains an N-terminal GST tag with a molecular weight of 64.6 kDa. (activemotif.com)
The assay is not affected by anticoagulation with standard or low molecular weight heparin or with warfarin. (medscape.com)
The goal of this RFA is to initiate a continuously evolving stream of scientifically and technologically outstanding assays that can be automated and used for screening at the Molecular Libraries Screening Centers. (nih.gov)
Emphasis will be placed on assays that provide insight into targets, either cellular or molecular, that have not been the focus of current HTS approaches. (nih.gov)
o Assays of cellular or molecular phenotypes. (nih.gov)

Sodium dodecyl sulfate (SDS), a common detergent, may be found in protein extracts because it is used to lyse cells by disrupting the membrane lipid bilayer and to denature proteins for SDS-PAGE. (wikipedia.org)
relies on the PLA technique, which can be applied to detect single proteins, protein isoforms, modified proteins or protein-protein complexes in either biofluids, such as plasma or serum, or in situ in fixed cells or tissues. (medscape.com)
These data largely confirmed that the multiplexing PLA assay was specific for the chosen binary protein complexes in the context of the PAE cells. (medscape.com)
This cryo-preservation method allows for the immediate dispensing of healthy, division-competent reporter cells into assay-ready plates. (indigobiosciences.com)
There is no need for intermediate treatment steps such as spin-and-rinse of cells, viability determinations or cell titer adjustments prior to assay setup. (indigobiosciences.com)
Dynamin-like proteins are membrane remodeling GTPases with well-understood functions in eukaryotic cells. (nature.com)
Folding confers to proteins the spatial characteristics that define their role in the cells, however the folding process might not always work properly. (selvita.com)
In this case proteins might accumulate and aggregate in cells thus becoming toxic. (selvita.com)
Investigators at the National Institute on Deafness and Other Communication Disorders (NIDCD) have developed an assay for the detection of protein-protein interactions in living cells. (nih.gov)
The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues. (fgf-erk.com)
We use Alkynyl Myristic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation, followed by click reaction with and azide-containing dye. (transcriptionfactor.org)

Simple simultaneous analysis of samples from 96- well microplates for filtration-based receptor binding assays. (calixar.com)
Saturation radioligand binding assays were performed on purified full-length wild-type A2A adenosine receptor with [3H]-CGS21680 agonist ( A ) or [3H]-ZM241385 inverse agonist ( B ). (calixar.com)
Because bile acids modulate signaling pathways for several nuclear receptors, this GPCR assay pairs well with INDIGO's existing assays for Farnesoid X Receptor (FXR) , Pregnane X Receptor (PXR) , and Vitamin D Receptor (VDR) . (indigobiosciences.com)

The researchers are determining the amount of protein in an antiserum in the assay plate. (nih.gov)
Researchers working on pre-clinical drug discovery related to bile acid metabolism and inflammation will find INDIGO's TGR5 assay very useful," said Bruce Sherf, PhD, INDIGO's Chief Technology Officer. (indigobiosciences.com)
INDIGO can also perform the TGR5 assay in its own lab as a service for researchers. (indigobiosciences.com)
It is assessed using a number of assays that help healthcare researchers gain a better understanding of the mechanisms and responses of the condition. (researchandmarkets.com)
Researchers at the Biodesign Institute of Arizona State University have developed a simple method for transitioning any protein, polypeptide, or peptide into a format for manipulation in a solution assay or experiment. (elsevierpure.com)

In the described multiplex in situ PLA, one of the PLA probes possesses a unique tag sequence identifier and functions as a linkage between the readout signal and the origin of this tag and thereby the targeted protein in the complex. (medscape.com)
By applying different tag sequences in the PLA probes and different fluorophores, the present study reports on multiplex detection of six binary protein complexes. (medscape.com)
By combining general PLA probes for EGFR, HER2 and HER3 with specific PLA probes for the same proteins, it was possible to detect all combinations of homo- and heterodimers of the three receptors. (medscape.com)

You can leverage CALIXAR's proprietary technology as well as other leading-in-class membrane protein technology that is not found anyplace else on the market. (calixar.com)
We are continuously integrating best-of-the-art membrane protein characterization technology for our clients' projects. (calixar.com)
Gain access to CALIXAR's Membrane Protein Expression Technology. (calixar.com)

It was adapted to a 96 well plate format to make it high â throughput, but this was not as effective as the previously developed fluorimeter assay due to the limitations of the plate reader and the transient nature of the transport readout. (manchester.ac.uk)
For example, it must be possible to reduce the assay to a 96-well or higher density format, and the assay should have a simple readout. (nih.gov)

INDIGO Biosciences is a leading provider of cell-based luciferase reporter assays. (indigobiosciences.com)

This has a significantly positive impact on the quality of the protein and on the success of proteomics, drug discovery applications (including vaccine, small molecules, and antibody development). (calixar.com)

For full details of the clinical application and interpretation of the assays, reference should be made to the PRU Handbook of Clinical Immunochemistry , (7th edition 2001) ISBN 0-948722-09-6. (sas-centre.org)
These limitations prevented it from becoming routine test in clinical setting and introduced many alternative methods, including flowcytometry-based NK cytotoxicity assay (FCA). (hindawi.com)
GICA specifically reacted with recombinant HA proteins, virus lysates, and clinical samples from H7 subtype viruses. (hindawi.com)

Covered methods are protein expression in E. coli , affinity chromatography, ion exchange chromatography, preparative and analytical gel filtration, crystallization, X-ray diffraction, and photometric assays. (jove.com)
This manuscript describes methods for FAHD protein expression in E. coli , affinity chromatography, ion exchange chromatography, ammonium sulfate precipitation, preparative and analytical gel filtration, crystallization, X-ray diffraction, and photometric assays. (jove.com)
The Andromeda system (Nanotemper) gives protein thermal unfolding data to help scientists to define optimal expression conditions that ensure the highest expression level together with the best thermal stability of membrane proteins. (calixar.com)
This combined approach guarantees that our Membrane Proteins are expressed in the best conditions with optimized yields and expression rates. (calixar.com)
It reduces/blocks the target gene expression through gene interference, to detect the accuracy of the antibody at recognising the target protein. (caltagmedsystems.co.uk)
Several polymorphisms of human P â glycoprotein have been associated with altered expression and drug resistance in vivo, but there is no consensus on their effect on the function of the protein. (manchester.ac.uk)

Unlike other protein assays, the Bradford protein assay is less susceptible to interference by various chemical compounds such as sodium, potassium or even carbohydrates like sucrose, that may be present in protein samples. (wikipedia.org)
Some colorless compounds such as proteins can be quantified at an Optical Density of 280 nm due to the presence of aromatic rings such as Tryptophan, Tyrosine and Phenylalanine but if none of these amino acids are present then the absorption cannot be measured at 280 nm. (wikipedia.org)
It is a protein that can bind and transport a wide variety of unrelated hydrophobic compounds. (manchester.ac.uk)
Selvita scientists have been working for many years on the production of different aggregation-prone proteins and to develop assays for screening compounds that are able to bind to pre-aggregated proteins or disturb their aggregation kinetic. (selvita.com)

The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. (wikipedia.org)
The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie brilliant blue G-250. (wikipedia.org)
During the formation of this complex, the red form of Coomassie dye first donates its free electron to the ionizable groups on the protein, which causes a disruption of the protein's native state, consequently exposing its hydrophobic pockets. (wikipedia.org)

All CosMx Protein assays undergo extensive antibody validation to ensure high quality data. (nanostring.com)
Proteins typically undergo a proper folding via intracellular chaperone machinery. (selvita.com)

The global oxidative stress assay market size reached US$ 719.3 Million in 2021. (researchandmarkets.com)

The APCR assay uses snake venoms to activate factor V and prothrombin, thus triggering the lower portion of the clotting pathway and eliminating interference by factor VIII and by lupus anticoagulants (in the absence of phospholipid complexes). (medscape.com)

Despite the capability of several common mass spectrometers to analyze many analytes at once (e.g., by using Multiple Reaction Monitoring), most of these assays have not been multiplexed. (nih.gov)

All CosMx Protein Assays are validated for use in multiplex, so you can focus on the biology of interest without wasting time troubleshooting assay performance. (nanostring.com)
Cloud-Clone also provides Multiplex Assay Kits which enable profiling of multiple proteins in a single well and custom services to cater to individual requirements. (caltagmedsystems.co.uk)
Cloud-Clone Multiplex assay kits enable fast and efficient profiling of multiple proteins in a single well using the Multiplex xMAP technology. (caltagmedsystems.co.uk)

Possibility to develop customized assays (transferable to our clients). (calixar.com)

Multiplexing assays provide more data from each sample of plasma, serum, or tissue culture supernatant than do ELISAs, a critical feature when sample size is limited. (caltagmedsystems.co.uk)
The laboratory treats a small slice of the tissue with the VENTANA PD-L1 (SP263) Assay. (fda.gov)
A study by Ellery et al indicated that in people with hemophilia, decreased factor VIII or IX levels may give rise to a compensatory procoagulant response, including an increase in platelet factor V. The investigators found that plasma protein S, platelet protein S, and plasma tissue factor pathway inhibitor-α were, respectively, one-third, 26%, and 9% lower than in controls, while platelet factor V was 50% higher. (medscape.com)

SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. (activemotif.com)
Here, we show that direct interaction between the inactive diguanylate cyclase (GGDEF) domain of PdeB and the FimV domain of the polar landmark protein HubP is crucial for full function of PdeB as a phosphodiesterase. (nature.com)
PdeB regulates abundance and activity of at least two crucial surface-interaction factors, the BpfA surface-adhesion protein and the MSHA type IV pilus. (nature.com)

The colloidal gold immunochromatographic assay (GICA) is a recently developed immunochromatographic technique for the identification of influenza A viruses with several notable advantages, such as the lack of requirement for any sample pretreatment, low sample volume requirement, ease of operation, rapid turnaround time, low cost, no cross-reactions, and no equipment requirements [ 11 ]. (hindawi.com)
Positive and negative control serum specimens are run simultaneously to allow identification of viral proteins. (cdc.gov)

Anatomy1

Organisms1

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes8

Information Science4

Antibodies14

Reagents8

Fluorescence6

Cell-Based Assays1

Reagent6

Purification7

Enzyme5

Specificity2

High throughput4

Mass spectrometry4

Reproducibility3

Colorimetric assay1

Neurodegenerative diseases3

Peptides2

BioAssay Systems2

Concentration12

Antibody assay1

Antigen2

ELISA1

Fluorescent3

Western-Blot1

Functional2

Calixar5

Quantitative determination of total protein2

Microtubules1

Robust4

ATPase3

Substrates1

Quantification1

Reproducible2

Proteomic analysis1

Ligand4

Serum Proteins1

Myosin3

Phosphatase1

Viral Culture3

Molecular6

Cells11

Receptor3

Researchers5

Probes3

Membrane Protein3

Readout2

Luciferase1

Drug Discovery1

Clinical3

Expression6

Compounds4

Coomassie3

Undergo2

20211

Phospholipid1

Analytes1

Multiplex3

Transferable1

Tissue3

Interaction3

Identification2