• Mounting evidence has demonstrated that AQP4, a water channel protein, is closely correlated with brain edema and could be an optimal therapeutic target for the reduction of ischemic brain edema. (benthamscience.com)
  • Mass photometry measurement of water channel aquaporin 4 (AQP4) sample solubilized using SMALPs. (the-scientist.com)
  • The target antigen of NMO-IgG was recently identified as aquaporin-4 (AQP4) water channel protein, which is mainly expressed in brain and spinal cord. (go.jp)
  • The water channel protein aquaporin-4 (AQP4) is expressed in astrocytes and mediates water flux across the blood-brain and blood-spinal cord barriers. (aston.ac.uk)
  • The organization of aquaporin -4 (AQP4) into large plasma membrane assemblies provides an opportunity to image membrane -bound AQP4 antibodies (AQP4- IgG ) and evaluate changes in their spatial distribution due to alterations in AQP4 isoform expression and AQP4- IgG epitope specificity . (bvsalud.org)
  • Preliminary study on the association of AQP4 promoter polymorphism with anti-aquaporin-4 antibody positivity in southern Han Chinese patients with idiopathic demyelinating disorders of central nervous system. (cdc.gov)
  • Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). (wikipedia.org)
  • Annotation of Selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants. (lu.se)
  • Phylogeny of major intrinsic proteins. (lu.se)
  • In HS, intrinsic defects in erythrocyte membrane proteins result in RBC cytoskeleton instability. (medscape.com)
  • This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. (antibodies-online.com)
  • Specific membrane intrinsic proteins of the aquaporin family can facilitate CO 2 diffusion across membranes. (unl.edu)
  • Membrane proteins: Aquaporins--channels without ions. (ox.ac.uk)
  • Hereditary spherocytosis (HS) is a familial hemolytic disorder associated with a variety of mutations that lead to defects in red blood cell (RBC) membrane proteins. (medscape.com)
  • Because membrane proteins represent 40 percent of drug targets, understanding them is crucial for drug discovery. (the-scientist.com)
  • Yet, performing structure-function analyses on membrane proteins is challenging because in their native states they are embedded within the plasma membrane's hydrophobic lipid bilayer. (the-scientist.com)
  • Because of this, membrane proteins are inherently unstable in aqueous solution, requiring distinct strategies for their solubilization, purification, and maintenance. (the-scientist.com)
  • 1 Mass photometry is a bioanalytical characterization technology that can help researchers characterize membrane protein properties, such as oligomerization or other complex formations and assess the purity of samples containing membrane proteins. (the-scientist.com)
  • Even after careful optimization, detergents do not necessarily resemble the native lipid bilayer, and researchers do not always obtain stably solubilized membrane proteins with intact functions. (the-scientist.com)
  • It is challenging for researchers to assess the quality of mimetics and identify which samples contain highly concentrated, stable membrane proteins. (the-scientist.com)
  • To overcome these difficulties, researchers working with membrane proteins would benefit from a method that can quickly and accurately characterize sample quality and composition. (the-scientist.com)
  • Mass photometry offers a powerful solution for the characterization of membrane proteins. (the-scientist.com)
  • Mass photometry provides the mass distribution of membrane proteins and other sample components at the single-molecule level within a few minutes using very little sample. (the-scientist.com)
  • Moreover, mass photometry works with a variety of membrane proteins and mimetics including detergents, nanodiscs, styrene maleic acid copolymers form self-assembling lipid-protein particles (SMALPs), and amphipols, making it compatible with extant membrane protein purification workflows. (the-scientist.com)
  • Recently determined structures have shed new light on the way that aquaporins act as passive, but selective, pores for the transport of small molecules--such as water or glycerol--across membranes. (ox.ac.uk)
  • Structure and Stability of the Spinach Aquaporin SoPIP2;1 in Detergent Micelles and Lipid Membranes. (lu.se)
  • Red cell membranes isolated from individuals with autosomal recessive HS have only 40-50% of the normal amount of spectrin (relative to band protein 3). (medscape.com)
  • Research associates at Amrita's Coimbatore campus have concluded that E.coli aquaporins can be effectively used to develop membranes for desalination of water. (amrita.edu)
  • Aquaporins or proteins embedded in cell membranes that regulate the flow of water in all living beings can be isolated to make membranes to do the job. (amrita.edu)
  • Approximately 25 percent of proteins localize to membranes, where they act as receptors and signaling molecules in biological processes, including respiration, neurotransmission, and molecular transport. (the-scientist.com)
  • 1 Aquaporins: Proteins that form pores in cell membranes to create water channels. (suntory.com)
  • At around the same time, a group at the University of Utah found that mammalian taste cells make proteins called aquaporins, which serve to channel water through cell membranes. (popsci.com)
  • Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)
  • In addition, basal expression levels of tight junction protein-1 encoding ZO1 were lower in R91W;Nrl −/− than in wt retinas. (nature.com)
  • Amyloid Precursor Protein (APP) Controls the Expression of the Transcriptional Activator Neuronal PAS Domain Protein 4 (NPAS4) and Synaptic GABA Release. (uclouvain.be)
  • Astrocytes may be recognized as such by their expression of glial fibrillary acidic protein, glutamine synthetase, glutamate transporter 1 (GLT1), aquaporin-4, aldehyde dehydrogenase 1 family member L1, and other proteins. (jneurosci.org)
  • Aquaporin-1 water channel protein in lung: ontogeny, steroid-induced expression, and distribution in rat. (hopkinsmedicine.org)
  • Expression of Aquaporin-1 water channel protein in mouse erythro-leukemia cells: genetic regulation of corticosteroid and chemical induction. (hopkinsmedicine.org)
  • Genomic organization and developmental expression of aquaporin-5 in lung. (hopkinsmedicine.org)
  • Keratinocyte growth factor modulates alveolar epithelial cell phenotype in vitro: expression of aquaporin-5 (AQP5). (hopkinsmedicine.org)
  • The effects of trifluoperazine on brain edema, aquaporin-4 expression and metabolic markers during the acute phase of stroke using photothrombotic mouse model. (lightsource.ca)
  • Western blot analysis detected expression of alpha amylase and aquaporin 5 protein, suggesting that primary cells were acinar in origin. (cdc.gov)
  • Association Between the Single Nucleotide Polymorphism and the Level of Aquaporin-4 Protein Expression in Han and Minority Chinese with Inflammatory Demyelinating Diseases of the Central Nervous System. (cdc.gov)
  • Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)
  • Role of Protein Kinases. (routledge.com)
  • Structural and functional studies of the glycerol channel aquaporin 7. (lu.se)
  • Aquaporin-9 Protein Is the Primary Route of Hepatocyte Glycerol Uptake for Glycerol Gluconeogenesis in Mice. (lu.se)
  • Our results delineate an approach to infer spatial relationships within protein arrays using stimulated emission depletion nanoscopy, offering insight into how information on single antibody fluorescence events can be used to extract information from dense protein assemblies under a biologic context. (bvsalud.org)
  • This review focuses on emerging topics encompassing the functional involvement of aquaporin channel proteins (AQPs) and membrane transport systems, also allowing permeation of NO and hydrogen peroxide, a major ROS, in oxidative stress physiology and pathophysiology. (hindawi.com)
  • This notion has been challenged by the discovery of new membrane transport functions, especially those exerted by aquaporins (AQPs), a family of membrane channel proteins widespread in nature [ 10 , 11 ]. (hindawi.com)
  • In this project, the aim of the research is to understand the roles of kinetoplastid aquaporins (AQPs) in trypanosomatid parasites with respect to drug resistance and transport. (gla.ac.uk)
  • Aquaporin-5 (AQP-5) is a protein that in humans is encoded by the AQP5 gene. (wikipedia.org)
  • AQP5+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH) Human AQP5 genome location and AQP5 gene details page in the UCSC Genome Browser. (wikipedia.org)
  • Abnormal distribution of AQP5 water channel protein in salivary glands from Sjgrens syndrome patients. (hopkinsmedicine.org)
  • 1] "The protective role of aquaporins in the freeze-tolerant insect Eurosta solidaginis: functional characterization and tissue abundance of EsAQP1. (tcdb.org)
  • Mass photometry advances membrane protein characterization by offering fast and precise mass measurements of samples containing membrane mimetics. (the-scientist.com)
  • Compared to other protein characterization techniques, such as SDS-PAGE and HPLC, mass photometry offers several unique advantages. (the-scientist.com)
  • One of the proteins, aquaporin, was developed into a recombinant tick aquaporin protein vaccine. (feedstuffs.com)
  • Aquaporin, Aqp1 in the gall fly. (tcdb.org)
  • Transcriptional regulation of aquaporins in accessions of Arabidopsis in response to drought stress. (lu.se)
  • The effects of the loss of TIP1;1 and TIP1;2 aquaporins in Arabidopsis thaliana. (lu.se)
  • Some of these aquaporins, such as PIP1;2 in Arabidopsis thaliana , have been suggested to exert control over g m and the magnitude of the CO 2 assimilation flux, but the evidence for a direct physiological role of aquaporins in determining g m is limited. (unl.edu)
  • This study presents the most in-depth analysis of g m in Arabidopsis aquaporin knock-out mutants to date. (unl.edu)
  • Background: Vasopressin induced trafficking of aquaporin-2 (AQP2) containing vesicles has been studied in kidney cell lines using conventional fluorescent proteins as tags. (johnshopkins.edu)
  • Unexpected complexity of the Aquaporin gene family in the moss Physcomitrella patens. (lu.se)
  • Researchers subsequently discovered a whole family of the proteins in animals, plants, and even bacteria. (britannica.com)
  • Aquaporin-5 (AQP-5) is a water channel protein. (wikipedia.org)
  • The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. (lu.se)
  • Reconstitution of water channel function and 2D-crystallization of human aquaporin 8. (lu.se)
  • Two different aquaporins were later found to play a major role in the mechanism by which human kidneys concentrate urine and return the extracted water to the blood. (britannica.com)
  • T3001Aquaporins are proteins that selectively conduct water molecules in and out of mammalian cells, while preventing the passage of ions and other solutes. (topsan.org)
  • Aquaporins are multifunctional water and solute transporters highly divergent in living organisms. (benthamscience.com)
  • Takata K, Matsuzaki T, Tajika Y. Aquaporins: water channel proteins of the cell membrane. (benthamscience.com)
  • He was elected to the American Society for Clinical Investigation in 2006 for work on aquaporin water channels. (hopkinsmedicine.org)
  • Dr. King's research focus early in his tenure was the biology of aquaporin water channel proteins in the respiratory tract as well as other tissues. (hopkinsmedicine.org)
  • Tunnels and channels facilitate the transport of small molecules, ions and water solvent in a large variety of proteins. (plos.org)
  • Aquaporins are involved in water circulation in humans and many other organisms. (suntory.com)
  • TFP acts on doughnut-shaped water channel proteins, called aquaporins, in brain cells. (lightsource.ca)
  • Water rushes from the outside through these doughnut-shaped proteins, into the cells that then swell," Dr. Salman said. (lightsource.ca)
  • The aquaporins, which are common in other types of cells, provide a possible way for water to stimulate taste cells directly. (popsci.com)
  • Aquaporins are proteins that regulate the flow of water in and out of cells. (lu.se)
  • diabetes insipidus: Types and causes: …in a gene known as AQP2 (aquaporin 2), which encodes a specific form of aquaporin. (britannica.com)
  • Characteristics of individual transport pathways, including their geometry, physico-chemical properties and dynamics are instrumental for understanding of structure-function relationships of these proteins, for the design of new inhibitors and construction of improved biocatalysts. (plos.org)
  • The structure of the yeast aquaporin that we have determined can be used to create inhibitors for human aquaporins, and this may in the long term lead to drugs that slow the growth of a cancer tumour", says Karin Lindkvist. (expertsvar.se)
  • Here, we propose a molecular mechanism where the AQP7 mobility in adipocytes is dependent on perilipin 1 and protein kinase A. Biochemical analyses combined with ex vivo studies in human primary adipocytes, demonstrate that perilipin 1 binds to AQP7, and that catecholamine activated protein kinase A phosphorylates the N-terminus of AQP7, thereby reducing complex formation. (lu.se)
  • An emerging theme is that TonEBP is a stress protein that mediates the cellular response to a range of pathological insults, including excess caloric intake, inflammation and oxidative stress. (nature.com)
  • Using a photoconvertible fluorescent protein tag on AQP2 might allow the simultaneous tracking of two separate populations of AQP2 vesicle after subcellular local photoconversion. (johnshopkins.edu)
  • Methods: A spacer was used to link a photoconvertible fluorescent protein (mEos2) to the amino-terminus of AQP2. (johnshopkins.edu)
  • Find Aquaporin 4 ELISA Kits for a variety of species such as anti-Cow Aquaporin 4, anti-Human Aquaporin 4, anti-Mouse Aquaporin 4. (antibodies-online.com)
  • For each protein, basic characteristics about its composition and length, its human relatives and relatedness to proteins in other species, and direct links to resources at NCBI are included. (cshlpress.com)
  • Additional links to NCBI resources are provided for human noncoding RNAs and repeated DNA elements and for proteins of interest from other species. (cshlpress.com)
  • The entire text of the Guide is searchable, and tools are available for identifying human protein sequences using those from other species. (cshlpress.com)
  • Vella J, Zammit C, Di Giovanni G, Muscat R, Valentino M. The central role of aquaporins in the pathophysiology of ischemic stroke. (benthamscience.com)
  • SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
  • Structural mechanism of plant aquaporin gating. (lu.se)
  • The study's results will be presented at the international conference Bioinformatica Indica 2010 during January 11-13, 2010 as a paper titled In-Silico Analysis (Structural and Sequence) of Aquaporins . (amrita.edu)
  • High-resolution x-ray structure of human aquaporin 5. (lu.se)
  • Amrita's study compared human and E.coli aquaporins and concluded that the latter were better. (amrita.edu)
  • Overexpression of wild-type human amyloid precursor protein alters GABAergic transmission. (uclouvain.be)
  • In the online version, these sections contain links to more information about proteins encoded by over 17,000 known or predicted human genes. (cshlpress.com)
  • To aid our understanding of the physiology of the epidermal barrier, we perform cell and molecular biology studies on proteins identified in association with rare skin diseases that manifest as barrier defects. (qmul.ac.uk)
  • Karin Lindkvist at the Department of Cell and Molecular Biology and Richard Neutze at the Department of Chemistry, University of Gothenburg have determined the three-dimensional structure of the yeast aquaporin. (expertsvar.se)
  • Spectrin deficiency can result from impaired synthesis of spectrin or from quantitative or qualitative deficiencies of other proteins that integrate spectrin into the red cell membrane. (medscape.com)
  • To preserve native membrane protein properties and structures, researchers typically use membrane mimetics to recreate a lipid environment in aqueous solutions. (the-scientist.com)
  • By quantifying the mass distribution of biomolecules in their samples, researchers gain valuable insights into sample purity, heterogeneity, and protein stoichiometry. (the-scientist.com)
  • Using mass photometry to count and measure the mass of the single membrane protein in solution allows researchers to gain valuable information on the purity and stability of their samples. (the-scientist.com)
  • Herein we present a new version of CAVER enabling automatic analysis of tunnels and channels in large ensembles of protein conformations. (plos.org)
  • TFP stops this from happening by preventing a signal that would normally cause more aquaporin channels to rise to the surface of brain cells. (lightsource.ca)
  • Cells strongly expressed the tight junction protein ZO-1 at points of cell-cell contact. (cdc.gov)
  • Additionally, selecting the best detergent to obtain large quantities of active, homogeneous, and stable protein requires laborious trial and error. (the-scientist.com)
  • Aquaporin-5 plays a role in the generation of saliva, tears and pulmonary secretions. (wikipedia.org)
  • Role of Aquaporins. (routledge.com)
  • In addition to its role in muscle tissue, it functions as an anchoring protein within the central nervous system such as in hippocampus and cerebellum. (frontiersin.org)
  • Role of aquaporin-4 in cerebral edema and stroke. (benthamscience.com)
  • Transport pathways play an essential role in the functioning of a large number of proteins. (plos.org)
  • These proteins have been found to play a role in both cancer and type 2 diabetes. (lu.se)
  • Centro Singular en Medicina Molecular y Enfermedades Crónicas. (fundacionareces.es)
  • It measures proteins' true molecular mass instead of relying on surrogate measures to predict molecular weights. (the-scientist.com)
  • Results indicated that the aquaporin protein was effective as an antigen in cattle vaccines to help prevent cattle fever tick infestations. (feedstuffs.com)
  • Dystrophin is part of a protein complex that connects the cytoskeleton to the extracellular matrix. (frontiersin.org)
  • What has remained, unclear is weather the TbAQP2 protein is acting as transmembrane transporter, while situated in the T. brucei flagellar pocket, with pentamidine being a permeant, or if the protein simply acts as a receptor for pentamidine to then be internalised together via receptor-mediated endocytosis or the natural turnover of TbAQP2. (gla.ac.uk)
  • Insights into Populus XIP aquaporins: evolutionary expansion, protein functionality, and environmental regulation. (inrae.fr)