• Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. (wikipedia.org)
  • Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. (wikipedia.org)
  • ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. (wikipedia.org)
  • You need info about Mouse Angiopoietin-related protein 2 (ANGPTL2) ELISA Kit or any other Gentaur produtct? (gentaurshop.com)
  • High levels of vascular endothelial growth factor (VEGF) and angiopoietin-2 activate rapid angiogenesis within the germinal matrix. (medscape.com)
  • We have previously shown that VWF itself regulates angiogenesis, raising the hypothesis that some of the angiogenic activity of VWF may be mediated by its interaction with Angpt-2. (imperial.ac.uk)
  • TSP-2 is a potent inhibitor of tumor growth and angiogenesis. (quanterix.com)
  • Angiopoietin (angpt) 1 and angpt-2 are circulating proteins first ascribed opposing roles in embryonic angiogenesis. (elsevierpure.com)
  • Cartilage oligomeric matrix protein-angiopoietin-1 (COMP-Ang1) is an angiogenic factor for vascular angiogenesis. (koreamed.org)
  • We can assist you in finding the right ELISA kits, PCR kits, recombinant proteins, antibodies, provide you with datasheets, manuals, kit protocols and other information about the products. (gentaurshop.com)
  • Recombinant Human TGFβ2 is a 25.0 kDa protein composed two identical 112 amino acid polypeptide chains linked by a single disulfide bond. (quanterix.com)
  • This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant Angiopoietin-3. (reliatech.de)
  • To test this, tubule formation and angiopoietins expression had been assessed in endothelial cells exposed to various concentrations of recombinant SARS-CoV-2 NSP7 Protein (His) C-6His neutropil elastase. (dna-alkylating.com)
  • The sivelestat regimen, optimized via a pharmacokinetics study, had potent effects on vascular stabilization by upregulating angiopoietin-1 through the AKT Recombinant?Proteins HPGDS Protein pathway and preventing tight junction protein degradation. (dna-alkylating.com)
  • Among the range of proteins shown to co-localize to WPB is angiopoietin-2 (Angpt-2), a ligand of the receptor tyrosine kinase Tie-2. (imperial.ac.uk)
  • Conversely, one report describes expansion of a biopsy-proven capillary malformation following partial surgical excision in an adult in whom the newly expanded capillary malformation expressed marked elevations of both tyrosine kinase receptor (Tie2) and its ligand angiopoietin-1 and no increase in VEGF. (medscape.com)
  • Type 2 diabetes mellitus (T2DM) is one of the common metabolic diseases in the world. (frontiersin.org)
  • Type 2 diabetes mellitus (T2DM) is a progressive metabolic disease characterized by pancreatic β-cell dysfunction and peripheral insulin resistance, leading to defects in glucose metabolism and chronic low-grade inflammation. (frontiersin.org)
  • Although dysregulated lipid metabolism results in diabetic nephropathy (DN) development in patients with type 2 diabetes mellitus (T2DM), it is not understood whether betatrophin is associated with urinary albumin excretion and renal function. (biomedcentral.com)
  • Circulating Angiopoietin-like Protein 8 Is Independently Associated With Fasting Plasma Glucose and Type 2 Diabetes Mellitus. (phoenixpeptide.com)
  • However, regulation of Angptl8 in human type 2 diabetes mellitus (T2DM) and renal dysfunction has not been determined. (phoenixpeptide.com)
  • Plasma levels of angiopoietin-2 (ANGPT2) and angiopoietin-like 4 protein (ANGPTL4) reflect different pathophysiological aspects of cardiovascular disease. (uib.no)
  • Some genetic causes of primary lymphedema are known and previous studies have shown that dominant-negative mutations in angiopoietin 2 (ANGPT2) promote lymphangiogenesis in mice. (genengnews.com)
  • The mutations result in loss of the normal function of the ANGPT2 protein that is known to play a role in lymphatic and blood vessel maturation. (genengnews.com)
  • The ANGPT2 encodes the angiopoietin 2 protein, a growth factor that binds to receptors in blood and lymphatic vessels. (genengnews.com)
  • The mutations that resulted in primary lymphedema in patients provided investigators important insights into the function of the ANGPT2 protein and mechanisms that lead to lymphedema. (genengnews.com)
  • A model of the angiopoietin 2 growth factor secretion from lymphatic endothelial cells and the effect of ANGPT2 mutations on the development of lymphedema. (genengnews.com)
  • Human Tie-1 cDNA encodes a 1138 amino acid (aa) residue precursor protein with a 24 residue putative signal peptide, a 735 residue extracellular domain and a 354 residue cytoplasmic domain. (rndsystems.com)
  • Mouse ANG 3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. (reliatech.de)
  • Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for the vascular endothelium. (wikipedia.org)
  • Given the contrasting effects of angpt-2 and angpt-1 on the vascular endothelium, these two factors may play an important role in the pathophysiology of septic shock in children, and further studies are warranted. (elsevierpure.com)
  • We hypothesized that neutrophil elastase is among the key determinants of vascular endothelium disruption/destabilization and impacts angiopoietins expression soon after spinal cord injury. (dna-alkylating.com)
  • Norata pointed out there are at least two approaches to targeting the protein, one a monoclonal antibody, evinacumab (Evkeeza, Regeneron Pharmaceuticals), that reduces the circulating form of ANGPTL3. (medscape.com)
  • A newly approved agent, evinacumab , is a monoclonal antibody targeted against angiopoietin-like protein 3 and is used as an adjunct with other lipid-lowering therapies for the treatment of homozygous familial hypercholesterolemia. (medscape.com)
  • We discovered LNA043-a derivative of angiopoietin-like 3 (ANGPTL3)-as a potent chondrogenesis inducer using a phenotypic screen with human mesenchymal stem cells. (nih.gov)
  • The drug, ARO-ANG3 (Arrowhead Pharmaceuticals), is a hepatocyte-targeted small interfering ribonucleic acid (siRNA) designed to silence expression of the angiopoietin-like protein 3 (ANGPTL3) gene. (medscape.com)
  • Approached for comment, G. Danilo Norata, PhD, professor, Department of Pharmacological and Biomolecular Sciences, University of Milan, Italy, said that ANGPTL3 is "emerging as an interesting protein in the field of dyslipidemia. (medscape.com)
  • Mouse Angiopoietin3 (ANG3), is a secreted glycoprotein belonging to the angiopoietin family. (reliatech.de)
  • To determine the temporal expression of vascular growth factors during the lifespan of the primate corpus luteum, experiments were designed to detect mRNA for vascular endothelial growth factor (VEGF), angiopoietin (Ang)-1 and Ang-2 and to localize protein expression for VEGF in macaque luteal tissue during the menstrual cycle. (oregonstate.edu)
  • Reverse transcription-polymerase chain reaction products equated to cDNA for VEGF, Ang-1 and Ang-2 in all corpora lutea. (oregonstate.edu)
  • These data suggest transcriptional control of VEGF, Ang-1 and Ang-2, as well as post-transcriptional control of VEGF, in macaque corpus luteum. (oregonstate.edu)
  • [ 6 ] These angiogenic vessels have few supporting pericytes, immature basal lamina, and reduced glial fibrillary acidic protein (GFAP) expression within the ensheathing astrocyte endfeet. (medscape.com)
  • The delirium-associated proteins identified as biomarker candidates are involved with inflammation, glial dysfunction, vascularization, and hemostasis, highlighting the multifactorial pathophysiology of delirium. (medrxiv.org)
  • Therefore, our result suggests that inhibiting neutropil elastase by administration of sivelestat is actually a promising therapeutic method to inhibit glial scar and promote functional recovery by upregulating angiopoietin-1 following spinal cord injury. (dna-alkylating.com)
  • Keyword phrases: Neutrophil elastase, Spinal cord injury, Glial scar, Angiopoietins, Functional recovery, Neuropathic painIntroduction Spinal cord injury (SCI) is usually a clinically devastating condition that could bring about either temporary or permanent disability in young adults [49, 76]. (dna-alkylating.com)
  • RESULTS: Static-binding assays revealed that Angpt-2 bound to VWF with high affinity (KD,app ∼3 nM) in a pH and calcium-dependent manner. (imperial.ac.uk)
  • The IgG2 fraction of the culture supernatant was purified by Protein A/G affinity chromatography. (reliatech.de)
  • For this reason, they are extensively used in a wide range of applications from protein production to bacterial biofilms and endothelial cell experiments. (imperial.ac.uk)
  • In this study, we aimed to identify plasma protein biomarkers and develop a predictive model for postoperative delirium in older patients undergoing cardiac surgery, while also uncovering possible pathophysiological mechanisms. (medrxiv.org)
  • SOMAscan analysis of 1,305 proteins in the plasma from 57 older adults undergoing cardiac surgery requiring cardiopulmonary bypass was conducted to define delirium-specific protein signatures at baseline (PREOP) and postoperative day 2 (POD2). (medrxiv.org)
  • Plasma levels of angpt-1 and angpt-2 were measured via a commercially available enzyme-linked immunosorbent assay. (elsevierpure.com)
  • Plasma angpt-2 levels were significantly elevated in children with septic shock when compared with healthy children, as well as critically ill children with either SIRS or sepsis, and circulating angpt-2 levels seemed to correlate with disease severity and outcome. (elsevierpure.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Mouse Angiopoietin Like Protein 8 (ANGPTL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (bioanalitica.it)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Angiopoietin Like Protein 8 (ANGPTL8) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (bioanalitica.it)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Angiopoietin Like Protein 8 (ANGPTL8) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (bioanalitica.it)
  • T ie-1/Tie and Tie-2/Tek are receptor tyrosine kinases with unique structural characteristics including two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains, followed by three fibronectin type III-like repeats in the extracellular region, and a split tyrosine kinase domain in the cytoplasmic region. (rndsystems.com)
  • They have also been shown to migrate to the cytoplasm to modulate cytoplasmic proteins and RNA function [ 12 ]. (biomedcentral.com)
  • In this study, we investigated a novel role of non-receptor protein tyrosine phosphatase N-2 (PTPN-2) in Ang-1 mediated stabilization of tight junction proteins. (johnshopkins.edu)
  • Angiopoietins (ANGPTs) are vascular development elements that play an essential function in vascular stabilization. (dna-alkylating.com)
  • Extracellular matrix is a fibrillar network composed of structural (collagens) and adhesion (fibronectin) proteins crucial for tissue architecture and biological pathway Histol Histopathol 27:1515-1528, 2012 . (springer.com)
  • This fibrillar network is composed of many structural proteins, such as collagens, and adhesion proteins, including fibronectin and proteoglycans (perlecan, decorin) [ 13 , 14 ]. (springer.com)
  • It has the characteristic structural motifs of angiopoietins including the coiled coiled domain near the amino-terminus and a fibrinogen like domain at the C-terminus. (reliatech.de)
  • In the developed vasculature, angpt-1 protects against vascular leak, whereas angpt-2 promotes increased vascular permeability. (elsevierpure.com)
  • Furthermore, we found that kidney fibrosis increases the circulating levels of the pro-angiogenic factor Angiopoietin 2 that increased vascular permeability of the lungs, but not the kidneys. (tmc.edu)
  • Interestingly, our study revealed that depletion of PTPN-2 by siRNAs abolishes Ang-1 ability to promote tyrosine dephosphorylation of Occludin, resulting Occludin disassociation from ZO-1 and endothelial hyperpermeability. (johnshopkins.edu)
  • Summary Collectively, our findings suggest that in brain endothelial cells blocking PTPN-2 mediated tyrosine phosphorylation of Occludin is a novel mechanism to maintain BBB function, and may offer a key therapeutic strategy for neuro-inflammatory disorders associated with BBB disruption. (johnshopkins.edu)
  • Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein. (cdc.gov)
  • Both bind the tyrosine kinase with immunoglobulin-like loop and epidermal growth factor homology domains (Tie) 2 receptor on endothelial cells, but angpt-1 is a Tie-2 agonist, whereas angpt-2 antagonizes Tie-2 signaling. (elsevierpure.com)
  • Obesity is defined as an expansion of white adipose (WAT) tissue that is associated with low grade inflammation which possibly contributes to the development of health complications such as diabetes, liver pathologies, dyslipidemia, cardiovascular diseases as well as some cancers [ 2 - 4 ]. (springer.com)
  • TSP-2 regulates collagen matrix formation by altering fibroblast behavior during development and in areas of tissue remodeling in the adult. (quanterix.com)
  • TSP-2 is a 150 kDa calcium-binding protein that regulates cellular interactions with extracellular matrix. (quanterix.com)
  • As expression of serum amyloid A, an acute phase protein, is known to play a role in the VS test classification, it is to be expected that the VeriStrat classification is associated with other proteins related to the acute response and/or chronic inflammation, as well. (biomedcentral.com)
  • SUMMARY Recently, it was reported that angiopoietin-like protein 8 (ANGPTL8) was the long-sought "betatrophin" that could control pancreatic beta cell proliferation. (phoenixpeptide.com)
  • Angiopoietin-like protein 8 (Angptl8) has recently been introduced as a novel adipokine/hepatokine that promotes pancreatic ß-cell proliferation and improves glucose tolerance in mouse models of insulin resistance. (phoenixpeptide.com)
  • Point mutations have a variety of effects on the downstream protein product consequences that are moderately predictable based upon the specifics of the mutation. (genengnews.com)
  • These consequences can range from benign (e.g. synonymous mutations) to catastrophic (e.g. frameshift mutations), with regard to protein production, composition, and function. (genengnews.com)
  • These ectatic vessels are lined by flat, benign-appearing endothelial cells, similar to the vessels of normal skin, with similar staining characteristics for endothelial antigens, including fibronectin, von Willebrand factor, and collagenous basement membrane proteins. (medscape.com)
  • So CCDS's gene number prediction represents a lower bound on the total number of human protein-coding genes. (wikipedia.org)
  • Human TGFβ exists as three isoforms (1,2,3), that are 70-80% homologous. (quanterix.com)
  • Elevated circulating lipasin/betatrophin in human type 2 diabetes and obesity. (phoenixpeptide.com)
  • Goat F(ab') 2 Anti-Rabbit IgG(H+L)-Alexa Fluor ® 555 antibody with minimal reactivity to mouse and human proteins for use in immunohistochemistry / immunocytochemistry and western blot assays. (southernbiotech.com)
  • Immunoglobulins were detected with serially diluted Goat F(ab') 2 Anti-Rabbit IgG(H+L), Mouse/Human ads-AF555 (SB Cat. (southernbiotech.com)
  • Tie-2 is a receptor for the angiopoietin (ANG) family: ANG-1, ANG-2, and ANG-3 (mouse)/-4 (human). (rndsystems.com)
  • CONCLUSIONS: Together, these data demonstrate a direct binding interaction between Angpt-2 and VWF that persists after secretion. (imperial.ac.uk)
  • Take the supernatant and measure the protein concentration mentioned in step2. (elabscience.com)
  • Centrifuge at 12,000 rpm for 2 min and collect the supernatant. (elabscience.com)
  • Our former study using gene expression microarray revealed the non-protein coding RNAs (ncRNAs) - nuclear-enriched Abundant Transcript 1 (NEAT1) elevated in peripheral blood from patients with CRC compared with healthy participants [ 8 ]. (biomedcentral.com)
  • Heparan sulfate proteoglycan 2 (Perlecan). (cdc.gov)
  • However, H2S reduced macrophage inflammatory protein-2 (MIP-2) release from unstretched macrophages (RAW264.7) co-cultured with stretched epithelial cells. (bvsalud.org)
  • Western blot analysis of extracts of various cell lines using Angiopoietin 2 Polyclonal Antibody at dilution of 1:3000. (elabscience.com)
  • The expression levels of neutropil elastase and angiopoietin-2 enhanced, and that of angiopoietin1 decreased after spinal cord injury in rats. (dna-alkylating.com)
  • Thrombospondin-2, C-reactive protein, TNF-receptor I, and placental growth factor were the analytes most highly associated with OS, all with Cox PH p -values ≤0.0001. (biomedcentral.com)
  • Following presentation of the interim analysis of this phase 2 trial, Arrowhead Pharmaceuticals announced that they are currently planning a phase 3 study "to further investigate ARO-ANG3" and that the company "intends to conduct a meeting with regulatory authorities in the second half of 2023 to discuss the proposed study design. (medscape.com)
  • Molecular cloning, expression, and characterization of angiopoietin-related protein. (wikipedia.org)
  • Changes in expression of vascular endothelial growth factor and angiopoietin-1 and -2 in the macaque corpus luteum during the menstrual cycle. (oregonstate.edu)
  • Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
  • In fact, while fibrotic lungs showed decreased expression of endothelial tight gap junction protein Claudin-5, the fibrotic kidneys had an elevated expression of Claudin-5. (tmc.edu)
  • Then, the expression of angiopoietin-1, angiopoietin-2, and neutrophil elastase was determined at 3 h and at 1, three, five, 7, 14, 21, and 28 days inside a clinically relevant model of moderate compression (35 g for 5 min at T10) spinal cord injury. (dna-alkylating.com)