• To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
  • With the development of next-generation sequencing (NGS), research has focused on comprehensive genomic analysis based on tumor molecular features, such as somatic mutations and copy number variation. (spandidos-publications.com)
  • Three partial genomic clones were generated and the sequence analysis revealed that they were identical at both nucleotide and amino acid levels. (scialert.net)
  • In silico identification by nucleotide sequence alignment between genomic and cDNA sequences revealed three putative introns in GBSS I. The longest intron was 121 bp long and was located between nucleotide 595 and 716. (scialert.net)
  • As a sub-objective, since PDE10A transcript variants were reported strictly through analyses of bovine genomic sequence, we also wanted to determine the nucleotide and amino acid sequences by experimental evidence. (plos.org)
  • Starting in the 90s, and the human genome project, genomic and mRNA sequences started to become more common in patents. (questel.com)
  • Sequence reads were assembled to the human reference genome using Burrows-Wheeler Aligner software (BWA) and the Genome Analysis Tool Kit (GATK) which identifies genomic variants. (umich.edu)
  • He also noted concern regarding delays in referring specimens to the laboratory for testing, and emphasized the need for improvement in timeliness of reporting and tracking of the viruses referred for genomic sequencing. (who.int)
  • He thanked the regional and global specialized laboratories for their efforts to provide timely results of the genomic sequencing of wild polioviruses, and for their help in planning and monitoring polio eradication activities in the Region. (who.int)
  • The NT gene (NTS) was sequenced for potential functional variants, evaluating its entire genomic and. (lu.se)
  • The NT gene (NTS) was sequenced for potential functional variants, evaluating its entire genomic and potentially regulatory regions, in DNA from 28 individuals, stratified by low and high pro-NT levels. (lu.se)
  • We compare all five currently sequenced Y. pestis genomes and the corresponding features in Yersinia pseudotuberculosis . (asm.org)
  • When two genomes from the same primate species are compared with each other, structural variants constitute 2 to 7 times more base pair differences than single nucleotide variants 2 . (nature.com)
  • Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on differentially expressed genes (DEGs). (spandidos-publications.com)
  • In a comparison excluding the composite sequencing data, the genomes and the open reading frames are fairly well conserved with differences only in 2, out of 80, genes. (usda.gov)
  • Only two ORFs, ICP4 and the US2 differed in amino acid (a.a.) lengths among the newly sequenced genomes. (usda.gov)
  • Exclusive to the genomes of the Serva vaccine strains, 7 non-synonymous mutation were identified in the predicted translation products of the genes encoding glycoproteins gB, gE, gL and gM and three nonstructural proteins UL28 (DNA packaging protein), UL5 (helicase-primase) and the immediate early protein ICP4. (usda.gov)
  • Overall our comparative sequence analysis of virulent GaHV-1 strains has identified both intragenic and intergenic deletions as well as single nucleotide polymorphism mutations that differentiate the Serva vaccine genome from the genomes of 4 virulent strains. (usda.gov)
  • L. H. Robert, Bonning B.C. (2003) Comparative analysis of the genomes of Rachiplusia ou and Autographa californica multiple nucleopolyhedroviruses Journal of General Virology 84, 1827-1842. (scirp.org)
  • Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)
  • Each run would cost about 8 to 9 thousand USD, thereby, de novo sequencing of mammalian genomes are in million dollar range. (wikidoc.org)
  • As of 2007, pyrosequencing is most commonly used for resequencing or sequencing of genomes for which the sequence of a close relative is already available. (wikidoc.org)
  • Six bat rotavirus genes (viral protein [VP] 2, VP6, VP7, nonstructural protein [NSP] 2, NSP3, and NSP5) shared ancestry with other mammalian rotaviruses but were distantly related. (cdc.gov)
  • Analysis of partial sequence of the VP1 gene indicated that it was distinct from cognate genes of other rotaviruses. (cdc.gov)
  • No sequences were obtained for the VP3 and NSP1 genes of the bat rotavirus. (cdc.gov)
  • Comparison of the genome sequences of phenotypically different strains could help to identify genes involved in virulence. (usda.gov)
  • Sequence features of the 13 protein-coding genes, two ribosomal RNAs and 22 transfer RNAs are described. (unboundmedicine.com)
  • The genetic study showed promising results in "silencing" the genes that translate into tau protein, the primary component behind dementia. (medicaldaily.com)
  • Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
  • It is now clear that Armadillo and beta-catenin bind directly to members of the T-cell factor/lymphoid enhancer factor subfamily of HMG box DNA-binding proteins, forming bipartite transcription factors that regulate Wingless/Wnt responsive genes in both Drosophila and vertebrates. (embl.de)
  • The genes are flanked on either side by highly conserved transcription start and stop signals and have intergenic sequences varying in length from 9 to 42 nt. (umd.edu)
  • We cloned and sequenced a 924-nt fragment that covered part of the 5′UTR and the VP4/VP2 capsid genes. (plos.org)
  • Bioinformatics strategies were used to find other homologous genes and proteins, which can also degrade HCH present in various bacteria for controlling of pollution and offer clue to prevent its entry in the food chain. (ispub.com)
  • Mutations causing growth defects in both vertebrate and tick hosts included in genes coding for a predicted alpha/beta hydrolase, a putative dicarboxylate amino acid:cation symporter, a T4SS protein, and predicted membrane-bound proteins. (bvsalud.org)
  • The tick non-essential genes included genes coding for many metabolic pathway- and outer membrane-associated proteins. (bvsalud.org)
  • Blood and the pleural fluid (purulent aspect) samples were subjected to qPCR (16S rRNA and dsb genes) and sequencing, which identified Ehrlichia canis and Anaplasma platys coinfection. (bvsalud.org)
  • Because longer genes encode longer BPRPs, we investigated the possibility of exon sequencing BPRP alleles encoded by PRB1 and PRB2. (umich.edu)
  • Of the 16 genes sequenced, 2 had a crossover between PRB1 and PRB2 and 4 had stop mutations. (umich.edu)
  • Current diagnostic PCR assays target a variety of SARS CoV-2 genes and the vast majority target sequences in regions of the SARS CoV-2 genome that are highly conserved. (who.int)
  • The S gene is one of the structural genes of the virus which encodes for a protein that sits on the surface of the SARS CoV-2 virus. (who.int)
  • There has been several studies related to the development and evolution of immunological processes, but a full scale genome and sequence based analysis has been carried out now, including 893 human genes involved in immune related processes. (lu.se)
  • Results of the analysis has been presented in the ImmTree database ( http://structure.bmc.lu.se/idbase/ImmTree ) which contains the trees and other related data for all the 893 genes. (lu.se)
  • GCS also uses expertise to identify specific genes and studies, as well as extensive sequencing genomics to explore how the variants may genetic profiles that contribute to the work. (who.int)
  • Sanger dideoxy sequencing, 454 pyrosequencing and Illumina sequencing were used to determine the nucleotide sequences of four groups of virulent GaHV-1 strains (groups I, III, V and VI). (usda.gov)
  • It also has two larger regions of unknown function, which are conserved in orthologs, and several key amino acids, the function of which in this particular protein has not yet been discovered. (wikipedia.org)
  • The Open reading frame (ORF) of the AnpeNPV consists of 738 nucleotides encoding 245 amino acids with molecular masses of 29 kDa. (scirp.org)
  • HM038439), was 731 nucleotides long and encoded a partial protein of 124 amino acids with predicted molecular weight of 13.65 kDa and calculated 10.01 isoelectric point. (scialert.net)
  • METHODS: We have used a combination of a multiple sequence alignment of orthologous BRCA1 sequences and a measure of the chemical difference between the amino acids present at individual residues in the sequence alignment to classify missense variants and in-frame deletions detected during mutation screening of BRCA1 . (xenbase.org)
  • When multiple sequence alignments were made, the zinc finger binding domains were the areas with the most conservation. (wikipedia.org)
  • Genome sequence alignments were used to identify two regions (5' terminus and the unique short/repeat junction) that contained various intragenic deletions. (usda.gov)
  • Simulate multi sequence alignments along phylogenetic trees. (haskell.org)
  • Handle evolutionary sequences and multi sequence alignments. (haskell.org)
  • force] [--no-elynx-file] COMMAND Analyze, and simulate multi sequence alignments. (haskell.org)
  • filter-columns Filter columns of multi sequence alignments. (haskell.org)
  • simulate Simulate multi sequence alignments. (haskell.org)
  • sub-sample Sub-sample columns from multi sequence alignments. (haskell.org)
  • Beside of the trees, other data like alignments of the sequences, statistics on nucleotide changes, domain and ontology data are also available. (lu.se)
  • Thus, eRF1 exploits two rRNA nucleotides also used during transfer RNA selection to drive messenger RNA compaction. (nature.com)
  • The unknown bacterium was readily distinguished from reference strains by 16S rRNA gene sequencing and biochemical tests. (microbiologyresearch.org)
  • Binding of eRF1 flips nucleotide A1825 of 18S ribosomal RNA so that it stacks on the second and third stop codon bases. (nature.com)
  • In serotype 4b, gtcA appears to be the first member of a bicistronic operon which includes a gene with homology to Bacillus subtilis rpmE , encoding ribosomal protein L31. (asm.org)
  • These ribosomal sequences was functional for the phylogenetic analysis and molecular taxonomic of any bacteria. (ispub.com)
  • We found 453 single nucleotide polymorphisms in protein-coding regions, which were used to assess the evolutionary relationships of these Y. pestis strains. (asm.org)
  • Within L. monocytogenes , sequences homologous to gtcA were found in all serogroup 4 isolates but not in strains of any other serotypes. (asm.org)
  • The nucleotide sequences of four virulent strains of gallid herpesvirus type 1, the causative agent of infectious laryngotracheitis, were determined. (usda.gov)
  • Two samples were co-infected with HRV-A and HRV-B or HRV-C. By comparative analysis of the VP4/VP2 sequences of the 66 HRVs, we showed a high diversity of strains in HRV-A and HRV-B species, and a prevalence of 51.5% of strains that belonged to the recently identified HRV-C species. (plos.org)
  • The Committee concluded that additional work may be needed to assess the viability of the stocks held in VECTOR, and that further molecular characterization of additional strains may be valuable in helping to identify strains from which further DNA sequences could be determined. (who.int)
  • RESULTS: In the present analysis we were able to classify an additional 50 missense variants and two in-frame deletions as probably deleterious and 92 missense variants as probably neutral. (xenbase.org)
  • Transient mismatch repair gene transfection for functional analysis of genetic hMLH1 and hMSH2 variants. (xenbase.org)
  • Genetic bioinformatic techniques to complement combines large population-based studies analysis comprises either candidate more traditional approaches for the study with laboratory and bioinformatics gene or genome-wide genotyping of rare genetic variants. (who.int)
  • To assess the performance of this new methodology, we performed a comprehensive comparison of AmpliSeq with RNA-seq using two well-established next-generation sequencing platforms (Illumina HiSeq and Ion Torrent Proton). (biomedcentral.com)
  • Methods: All exons of PRB1 and PRB2 from 8 healthy adults were sequenced using an Illumina HiSeq 2000 (100 bp paired sequencing readouts with TruSeq™ Exome Enrichment Kit). (umich.edu)
  • In an analysis of synonymous and non-synonymous mutations, it was discovered that non-synonymous mutation in glycoproteins B, E, L and M were exclusively found in the vaccine strain Serva. (usda.gov)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Sequence analysis of BRCA1 and BRCA2: correlation of mutations with family history and ovarian cancer risk. (xenbase.org)
  • Three mutations caused the bacterial defective growth only in the tick vector, which represented putative membrane proteins. (bvsalud.org)
  • Some mutations in the S gene may lead to changes in the spike protein which result in inhibition of contact and entry of the virus into human cells, however in the case of the VOC, they contain mutations in the S gene that enhance the process of contact and entry into human cells, increasing transmissibility of the virus. (who.int)
  • Analysis of these large elucidatinG the etioloGical role sites, and also patterns of genetic genome-wide studies also includes a of obeSity and related riSk factorS mutations that are observed in tumours. (who.int)
  • In conclusion, putative peanut trypsin inhibitors have been isolated and reveal high similiarity at the nucleotide and amino acid level to peanut allergen Ara h 3 and Ara h 4. (usda.gov)
  • Results: Three putative positive clones were isolated, purified, subcloned and sequenced. (usda.gov)
  • One assembled sequence of 5,662 nucleotides, believed to be a complete viral genome, encodes a single open reading frame containing a RdRp and a putative capsid protein similar to that of the positive-strand RNA containing nodaviruses, tetraviruses, and birnaviruses. (virology.ws)
  • Whole exome sequencing was carried out on an Italian family with moyamoya-complicated neurofibromatosis type 1 to identify putative genetic modifiers independent of the NF1 locus and potentially involved in moyamoya pathogenesis. (cancerindex.org)
  • These include early mathematical models of virus-host interaction, and more recently the analysis of viral nucleotide and protein sequences to track their function, epidemiology, and evolution. (doe.gov)
  • Direct sequencing of viral communities from the environment, known as viral metagenomics, is one approach being taken to discover archaeal viruses. (virology.ws)
  • Analysis of the RNA viral sequences revealed coding regions for a predicted RNA dependent RNA polymerase (RdRp), a hallmark of RNA viruses. (virology.ws)
  • Another viral sequence encoded a protein with 70% amino acid homology to the predicted RdRp. (virology.ws)
  • The viral S gene is important as it codes for the Spike protein which is the molecule that makes contact with, and allows entry of the virus into susceptible host cells, causing infection. (who.int)
  • Looking at the intron sequences next to the beginning and end of each exon and comparing those to favorable ones, it was found that all of the splice regions on ZNF800 are favorable. (wikipedia.org)
  • While whole gene duplications and deletions are relatively well-studied, the biology of subexonic ( i.e. , within coding exon sequences), copy number variation remains elusive. (nature.com)
  • From a series of 20,000 full sequence tests of BRCA1 carried out at Myriad Genetic Laboratories, a total of 314 different missense changes and eight in-frame deletions were observed. (xenbase.org)
  • Historical trend of newly published sequence patents from 2000 to 2020 available in Orbit BioSequence. (questel.com)
  • A total of six pairs of cancerous and adjacent healthy tissue were obtained from patients with HNSCC and genome‑wide transcriptional sequencing was performed. (spandidos-publications.com)
  • E. A. van Strien, D. Zuidema, R.W. Goldbach, Vlak, J. M. (1992) Nucleotide sequence and transcriptional analysis of the polyhedrin gene of Spodoptera exigua nuclear polyhedrosis vi-rus. (scirp.org)
  • The sequence reads were analysed using CLC Genomics Workbench 6.0 and phylogenetic trees were constructed using PhyML version 3.0. (scielo.br)
  • The genomics revolution has provided an unprecedented amount of sequence information from both viruses and their hosts. (doe.gov)
  • Two recombinant phage, HJ1 and HJ4, containing homologous inserts of 350 and 600 bp, respectively, produced proteins that reacted with antibody. (ncsu.edu)
  • Sixteen single-nucleotide polymorphisms (SNPs) were genotyped at and around the G72/G30 locus, which covered a 157-kb region encompassing the entire complementary DNA sequences of G72 and G30. (nih.gov)
  • Nucleotide sequences contained numerous single nucleotide polymorphisms (SNPs), including amino acid to stop codon conversions and crossover events that deleted portions of PRB1 and/or PRB2. (umich.edu)
  • Bioinformatics tools are software programs that are designed for extracting the meaningful information from the mass of molecular biology/biological databases and carry out sequence or structural analysis. (academicjournals.org)
  • Examine, modify, and simulate molecular sequences in a reproducible way. (haskell.org)
  • As a first step towards understanding the molecular genetics and pathogenicity of APMV-4, we have sequenced the complete genome of APMV-4 strain duck/Hong Kong/D3/75 and determined its pathogenicity in embryonated chicken eggs. (umd.edu)
  • Transcriptome analysis has enhanced our understanding of the molecular constituents of cells and tissues. (biomedcentral.com)
  • Methods: Thirty-two degenerate oligonucleotides DNA primers of 24 nucleotides each were synthesized based on the published amino acid sequences of peanut BBTI. (usda.gov)
  • Recently, metagenomics a new approach to identify culturable and non culturable bacteria with the help of 16S rDNA sequences isolate total DNA from HCH contaminated soil and amplified 16S rDNA through universal primers and confirmed its total population with denaturing gradient gel electrophoresis and sequencing. (ispub.com)
  • 7 Primers designed to amplify the partial groEL gene encoding heat-shock protein of Anaplasma phagocytophilum EphplgroELF (5′-ATGGTATGCAGTTTGATCGC-3′) and EphplgroELR (5′-TCTACTCTGTCTTTGCGTTC-3′) were used and expected to yield a 625-bp product for Anaplasma phagocytophilum and for Anaplasma platys , respectively. (who.int)
  • Direct DNA sequencing was performed using the same PCR primers. (who.int)
  • If the sequence result was of low quality, the amplicon was cloned into a plasmid vector using a TOPO TA cloning kit (Invitrogen, Carlsbad, California) and then sequenced using the primers provided with the kit. (who.int)
  • and 3) To isolate, sequence and analyze at least one resulting positive peanut trypsin inhibitor clone. (usda.gov)
  • The effect of dialysis on shear stress and shear strain at failure of whey protein concentrate/isolate gels. (ncsu.edu)
  • In addition, in silico analysis of FI proteins suggests that they represent recent acquisitions of conjugative plasmids, i.e., are not shared by members of the same plasmid species. (frontiersin.org)
  • Epidemiology Group (GEP) is to including in silico, expression, and work over the 2016-2017 biennium are contribute to understanding the causes sequencing studies, which are often described here. (who.int)
  • The genome sequence of the multinucleocapsid nucleo-polyhedrovirus of the Chinese oak silkworm Antheraea pernyi. (scirp.org)
  • Real-time DNA sequencing using detection of pyrophosphate release. (wikidoc.org)
  • The objective of this document is to outline the available methods and tools for screening and sequencing patient specimens for the detection of VOC or VOI, with a focus on those that offer a rapid turn-around time so as to support rapid implementation of public health actions for COVID 19 control. (who.int)
  • This gene encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. (cancerindex.org)
  • ABSTRACT The kinetic compensation effect in the heat denaturation of whey proteins was demonstrated using two different kinetic compensation relationships, i.e. (ncsu.edu)
  • Our designed tool is a set of search programs for the windows platform and is used to perform fast searches for comparison of nucleotide sequences, mutation identification and single nucleotide polymorphism (SNP). (academicjournals.org)
  • Sequence analysis revealed a partial cDNA clone of 643 bp with a start codon and with 96% and 93% homology with peanut allergens Ara h 4 and Ara h 3 cDNA clones, respectively. (usda.gov)
  • ZNF800 contains 6 functioning C2H2 zinc finger protein domains, a number of SNP's, and multiple experimentally-shown phosphorylation and SUMOylation sites. (wikipedia.org)
  • These were compared to experimentally shown phosphorylation sites found on NCBI Nucleotide and matching phosphorylation sites were bolded. (wikipedia.org)
  • it is generally believed that all the copies in an organism are identical or nearly identical in nucleotide sequence. (ispub.com)
  • A secondary goal that should be sought by a bioinformatics facility is to make algorithms and analysis methods accessible for non-computational biologists. (lu.se)
  • METHODS For the sample detected as G10P[14], 10% suspension was prepared and used for RNA extraction and sequence independent amplification. (scielo.br)
  • All three statistical methods demonstrate AmpliSeq as a highly accurate method for differential gene expression analysis. (biomedcentral.com)
  • Currently, a limitation of the method is that the lengths of individual reads of DNA sequence are in the neighborhood of 300-500 nucleotides, shorter than the 800-1000 obtainable with chain termination methods (e.g. (wikidoc.org)
  • Hence, any substantial structural perturbation due to salt, pH, alcohol, or heat treatment leads to irreversible changes in protein structure, even if a simulated physiological environment is restored. (ncsu.edu)
  • We confirmed the close relationship of their structural proteins but showed apparent additional recombination events in the 2A gene and 3′UTR of the HRV-Ca strain. (plos.org)
  • When analyzing a fragment of the 5′ UTR, we characterized at least two subspecies of HRV-C: HRV-Cc, which clustered differently from HRV-A and HRV-B, and HRV-Ca, which resulted from previous recombination in this region with sequences related to HRV-A. The full-length sequence of one strain of each HRV-Ca and HRV-Cc subspecies was obtained for comparative analysis. (plos.org)
  • Soil Virus Offers Insight into Maintaining Microorganisms Through a collaborative effort, researchers have identified a protein in soil viruses that may promote soil health. (doe.gov)
  • However whether or not the hosts of these viruses are Archaea or Bacteria cannot be determined by these metagenomic analyses. (virology.ws)
  • Phylogenic analysis of the nucleotide sequences of viruses of all five genera of the family Paramyxoviridae showed that APMV-4 is more closely related to the APMVs than to other paramyxoviruses, reinforcing the classification of all APMVs in the genus Avulavirus of the family Paramyxoviridae. (umd.edu)
  • If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)
  • Phylogenetic analysis revealed that cassava GBSS I belongs to dicot subgroup and is closely related to GBSS I of potato, sweet potato and buckthorn. (scialert.net)
  • Phylogenetic analysis showed that 7 out of 9 of haplotypes bearing three copies grouped in a separate cluster. (unboundmedicine.com)
  • Phylogenetic analysis with DNA amplification technologies. (who.int)
  • Several techniques based on the polymerase chain reaction (PCR) to facilitate the characterization and phylogenetic analysis of variola virus isolates were described. (who.int)
  • On the basis of 16S rDNA phylogenetic analysis of bacteria was done. (ispub.com)
  • Bacterial toxins can be single proteins or oligomeric protein complexes that are organized with distinct AB structure-function properties. (springer.com)
  • Short sequence reads are generated by either single-end or paired-end sequencing. (biomedcentral.com)
  • Predicting the functional consequences of non-synonymous single nucleotide polymorphisms: structure-based assessment of amino acid variation. (xenbase.org)
  • Characterization of disease-associated single amino acid polymorphisms in terms of sequence and structure properties. (xenbase.org)
  • Latest platform of Pyrosequencing (GS FLX from 454 Life Sciences which is owned by Roche) can generate 100 million nucleotide data in a 7 hour run with a single machine. (wikidoc.org)
  • Essentially, the method allows sequencing of a single strand of DNA by synthesizing the complementary strand along it. (wikidoc.org)
  • Improved performance of Pyrosequencing using single-stranded DNA-binding protein. (wikidoc.org)
  • Termination of protein synthesis occurs when a translating ribosome encounters one of three universally conserved stop codons: UAA, UAG or UGA. (nature.com)
  • Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle developed by Mostafa Ronaghi and Pål Nyrén (published in Analytical Biochemistry 1996 and Science 1998). (wikidoc.org)
  • Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
  • To examine the role of protein kinase C as a chronic regulator of proximal tubule Na/H antiporter activity, the effect of phorbol 12-myristate 13-acetate (PMA) on the Na/H antiporter was studied in cultured proximal tubule cells. (jci.org)
  • Short-term activation of protein kinase C by 5 min exposure to PMA caused an acute increase in Na/H antiporter activity that was not prevented by cycloheximide or actinomycin D and did not persist 24 h later. (jci.org)
  • Long-term activation of protein kinase C by 2 h exposure to PMA caused a dose-dependent increase in Na/H antiporter activity 24 h later. (jci.org)
  • This latter effect was due to protein kinase C activation in that it was inhibited by sphingosine and was not seen with 4 alpha-PMA, an inactive analogue. (jci.org)
  • In conclusion, short-term activation of protein kinase C leads to a transient increase in Na/H antiporter activity that is independent of transcription and translation, whereas long-term activation of protein kinase C causes a persistent increase in antiporter activity that is dependent on transcription and translation and is associated with increased mRNANa/H abundance. (jci.org)
  • These encoding the protein kinase US2 and the immediate early protein ICP4. (usda.gov)
  • Gupta DD, Saha S, Chakrabarti MK (2005) Involvement of protein kinase C in the mechanism of action of Escherichia coli heat-stable enterotoxin (STa) in a human colonic carcinoma cell line, COLO-205. (springer.com)
  • W. B. Wang, S. Y. Zhu, L. Q. Wang, F. Yu, Shen W. D. (2005) Cloning and sequence analysis of the Antheraea pernyi nucleo-polyhedrovirus gp64 gene. (scirp.org)
  • Cloning and expression of the gene for lactacin F, a Lactobacillus acidophilus bacteriocin, using an amino acid sequence-derived DNA probe. (ncsu.edu)
  • Site-specific integration in Lactobacillus acidophilus ADH: Cloning and sequence analysis of the attachment site of the bacteriophage phiadh. (ncsu.edu)
  • CLONING, EXPRESSION, AND NUCLEOTIDE-SEQUENCE OF THE LACTOBACILLUS-HELVETICUS-481 GENE ENCODING THE BACTERIOCIN HELVETICIN-J. JOURNAL OF BACTERIOLOGY, 172(11), 6339–6347. (ncsu.edu)
  • The salivary MUC7 gene provides an opportunity for studying such variation, as it harbors copy number variable subexonic repeat sequences that encode for densely O-glycosylated domains (PTS-repeats) with microbe-binding properties. (nature.com)
  • ORF2 could encode an 11,808-Da protein. (ncsu.edu)
  • ORF3 could encode a 37,511-Da protein. (ncsu.edu)
  • Expression of the polh gene of AnpeNPV in E. coli was examined by SDS-PAGE, Western blot and Mass-spectrum analysis. (scirp.org)
  • S. Gomi, C. E. Zhou, W. Y. Yih, K. Majima, Maeda S. (1997) Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV. (scirp.org)
  • Although a high level of PDE10A gene expression is observed in the testis, information on the identity of the isoforms or on the cell type that express the PDE10 protein is lacking. (plos.org)
  • Whole transcriptome sequencing (RNA-seq) represents a powerful approach for whole transcriptome gene expression analysis. (biomedcentral.com)
  • Our study indicates that AmpliSeq excels in the limiting areas of RNA-seq for gene expression quantification analysis. (biomedcentral.com)
  • Thus, AmpliSeq stands as a very sensitive and cost-effective approach for very large scale gene expression analysis and mRNA marker screening with high accuracy. (biomedcentral.com)
  • Though accepted as the gold-standard for measuring gene expression, this method is lower throughput and not amenable to perform global expression analyses. (biomedcentral.com)
  • The number of sequencing reads that map to each transcript is used to infer the abundance of mRNA molecules. (biomedcentral.com)
  • Circular RNAs (circRNAs) are closed, long, non-coding RNAs that are formed via direct reverse splicing of precursor mRNA that function by regulating RNA transcription and protein production, as well as sponging miRNAs ( 4 ). (spandidos-publications.com)
  • Whole-transcriptome sequencing (RNA-seq) technology has emerged as a revolutionary platform for genome-wide quantification of mRNA transcripts. (biomedcentral.com)
  • The nuclear localization sequences of the BRCA1 protein interact with the importin-alpha subunit of the nuclear transport signal receptor. (xenbase.org)
  • Third, unless your sequence is very short, you will always want to find sequences similar to yours, not just identical. (questel.com)
  • Membrane structure and function: Structure of model membrane, lipid bilayer and membrane protein diffusion, osmosis, ion channels, active transport, ion pumps, mechanism of sorting and regulation of intracellular transport, electrical properties of membranes. (pathfinderacademy.in)
  • In the Fp subunit of SQR, and the closely related membrane-associated fumarate reductase of anaerobic organisms, FAD is covalently attached via an 8α-N(3)-histidyl linkage to a histidine (His) in a conserved sequence in the N-terminal part of the polypeptide. (lu.se)
  • Amino acid substitution during functionally constrained divergent evolution of protein sequences. (xenbase.org)
  • Most notably, are the applications for genome sequencing and metagenomics. (wikidoc.org)
  • On the contrary, only two (93.8% of haplotypes) or three copies (6.2%) of an 8-bp repeated sequence motif was found in S. senegalensis. (unboundmedicine.com)
  • The armadillo (Arm) repeat is an approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila melanogaster segment polarity gene armadillo involved in signal transduction through wingless. (embl.de)
  • At first it was hypothesized that ZNF800 has an Ortholog in fungus, dating back to 1150 Millions of years ago, however, a BLAT search of the fungus sequence in the human domain gave no results, which lead to the conclusion that these sequences are not similar enough to prove they are truly related. (wikipedia.org)
  • 325 open reading frames (ORFs) were compared with those of the recently sequenced genome of the Serva vaccine strain. (usda.gov)
  • FINDINGS The next generation sequencing and phylogenetic analyses of the 11-segmented genome of the G10P[14] strain allowed classification as G10-P[14]-I2-R2-C2-M2-A3-N2-T6-E2-H3. (scielo.br)
  • Takao T, Hitouji T, Aimoto S, Shimonishi Y, Hara S, Takeda T, Takeda Y, Miwatani T (1983) Amino acid sequence of a heat-stable enterotoxin isolated from enterotoxigentic Escherichia coli strain 18D. (springer.com)
  • This protein is a critical in the linking of the virus to a host cell it can infect. (who.int)
  • Two complete open reading frames (ORF), designated ORF2 and ORF3, were identified within the sequenced fragment. (ncsu.edu)
  • Polymerase chain reaction (PCR) was conducted using groEL PCR-restriction fragment length polymorphism and sequence analysis. (who.int)
  • A core region of beta-catenin, composed of 12 copies of a 42 amino acid sequence motif known as an armadillo repeat, mediates these interactions. (embl.de)
  • Goupil S, Maréchal L, El Hajj H, Tremblay M-È, Richard FJ, Leclerc P (2016) Identification and Localization of the Cyclic Nucleotide Phosphodiesterase 10A in Bovine Testis and Mature Spermatozoa. (plos.org)
  • Several different tools were used on ExPASy Proteomics to analyze ZNF800 for likely protein modification sites, unique composition, and localization. (wikipedia.org)
  • Atomic coordinates have been deposited with the Protein Data Bank under accession codes 3JAG , 3JAH and 3JAI . (nature.com)
  • Analysis of the sequencing data revealed numerous discrepancies with the published sequence of a composite genome. (usda.gov)
  • A Better Way to Find RNA Virus Needles in the Proverbial Database Haystacks Researchers combed through more than 5,000 data sets of RNA sequences generated from diverse environmental samples around the world, resulting in a five-fold increase of RNA virus diversity. (doe.gov)
  • Data integrity is verified using SHA256 sums so that validation of past analyses is possible without the need to recompute the result. (haskell.org)
  • no-elynx-file Do not write data required to reproduce an analysis. (haskell.org)
  • If data is a multi sequence alignment, additionally analyze columns. (haskell.org)
  • http://www.nserc-crsng.gc.ca ) to FJR and PL. This funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
  • This study has been carried out a data mining centered analysis based on the existed data of NCBI's HomoloGene and TIGR's EGO databases. (lu.se)
  • Sperm intracellular cAMP levels depend on the activity of adenylyl cyclases, mostly SACY, though transmembrane-containing adenylyl cyclases are also present, and on the activity of cyclic nucleotide phosphodiesterases (PDE) whose role is to degrade cAMP into 5'-AMP. (plos.org)
  • The cleavage site of the F protein (DIPQR↓F) does not conform to the preferred cleavage site of the ubiquitous intracellular protease furin. (umd.edu)
  • A subset of these proteins is conserved across eukaryotic kingdoms. (embl.de)
  • A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor. (nature.com)
  • The adenomatous polyposis coli tumor suppressor protein is also implicated in beta-catenin signaling. (embl.de)