• The thyroid hormones (THs)-thyroxine (T4) and 3,5,3'-triiodothyronine (T3)-circulate in blood by reversibly binding to carrier proteins. (medscape.com)
  • How to Measure the Similarity Between Protein Ligand-binding Sites. (uni-marburg.de)
  • The Automatic Search for Ligand Binding Sites in Proteins of Known Three-dimensional Structure Using only Geometric Criteria. (uni-marburg.de)
  • A simple and fuzzy method to align and compare druggable ligand-binding sites. (uni-marburg.de)
  • By fragment screening using x-ray crystallography we identified four ligands revealing ligand-binding sites in conserved interfaces between SARS-CoV-2 nsp10 and nsp14/nsp16. (lu.se)
  • The mature protein comprises around 165 residues, and contains a number of potential N-glycosylation sites. (wikipedia.org)
  • VH is derived from a single protein domain of 35 residues [ 9 ]. (hindawi.com)
  • Accurate prediction of critical residues along with their specific and quantitative contributions to protein-protein binding free energy is extremely helpful to reveal binding mechanisms and identify drug-like molecules that alter protein-protein interactions. (utoronto.ca)
  • The approach provides explicit contribution of the entropic loss in binding free energy of individual residues directly from fluctuation of the interaction energy in MD simulation. (utoronto.ca)
  • Immobilized Metal Ion Affinity Chromatography (IMAC), developed by Porath (1), is based on the interaction of certain protein residues (histidines, cysteines, and to some extent tryptophans) with cations of transition metals. (gbiosciences.com)
  • This resin binds to six histidine residues (6x His), a common tag used in protein purification. (gbiosciences.com)
  • Thin-layer chromatography of dinitrophenyl derivatives prepared from Pronase-digested microsomal protein and sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis of solubilized microsomes indicated that the radiolabeled products were covalently bound to amino acid residues of microsomal protein. (aspetjournals.org)
  • Pumby adapts naturally occurring proteins called Pumilio homology domains (PumHDs), which consist of amino acids that bind to each of the four different ribonucleotide bases that make up RNA sequences. (genengnews.com)
  • First, they designed two Pumby proteins that would bind to adjacent RNA sequences. (genengnews.com)
  • The binding of trinucleoside diphosphates to long helical rods of tobacco mosaic virus (TMV) protein is shown to depend on base sequence, 5′ AAG 3′ binding being the strongest of the 25 trinucleoside diphosphate sequences measured. (nature.com)
  • From our binding constant data the most likely frame is predicted and shown to have two contiguous AAG sequences in a hairpin loop region. (nature.com)
  • Protein design methods use trial and error or more sophisticated methods like directed evolution or inverse folding to generate novel scaffolds or to find novel protein sequences folding into a defined scaffold, respectively. (hindawi.com)
  • Given the intimate relationship between a protein's structure and function, a way to design proteins with targeted properties is to start from a desired structure and find sequences able to fold into it, imposing additional constraints in the process [ 1 ]. (hindawi.com)
  • Similarities can be present at various levels which can be detected through comparison of protein sequences or their structural folds. (nih.gov)
  • It is a nuclear protein that is largely chromatin-bound and has a strong preference for binding to methylated DNA sequences in vivo . (portlandpress.com)
  • Cobalt resins have a higher selectivity for poly-His sequences, however have a low loading capacity, therefore Cobalt Chelating Resin should be used for valuable recombinant proteins in limited quantities. (gbiosciences.com)
  • Selective microfluidics-based ligand enrichment followed by sequencing, or SMiLE-seq, combines antibody arrays, mechanical trapping, and next-generation sequencing readouts to provide a new platform for characterizing DNA-protein interactions. (genomeweb.com)
  • By physically restraining bound molecules against an antibody array, they capture both transient interactions, which yeast two-hybrid or tandem mass spectrometry methods don't, and weak interactions, which are usually obliterated by the wash in protein-DNA microarrays. (genomeweb.com)
  • By MALDI-TOF mass spectrometry, we identified this protein to be cMyBP-C. These data were confirmed by immunostaining using the p-PKD-Ser744/748 antibody, which displayed a striated pattern similar to the one observ ed for a regular cMyBP-C antibody. (scirp.org)
  • Antibody responses targeting the M protein have been shown to favor the conserved C region. (lu.se)
  • However, analogous proteins may have structural homology although this is not a prerequisite. (hindawi.com)
  • We combine NMR relaxation experiments with molecular dynamics (MD) simulations to derive a highly detailed picture of how the conformational entropy changes when the protein binds different ligands. (lu.se)
  • It is suggested that Chip cooperates with different LIM domain proteins and other factors to structurally support remote enhancer-promoter interactions. (nih.gov)
  • Protein-protein interactions are a common biochemical process, important in a great many biological systems, and are controlled by their molecular structure. (thenakedscientists.com)
  • Hydrophobicity was one of the most important physicochemical properties, due to the fact that it is involved in protein interactions, for example, by forming hydrophobic cores. (hindawi.com)
  • Possible DNA-binding interactions are discussed on the basis of comparisons to other winged helix proteins. (rcsb.org)
  • That year, the two published a paper in Science describing a method to trap low-affinity DNA-protein interactions, specifically those of transcription factors. (genomeweb.com)
  • The transcription factors bind to any number of DNA motifs in the library, those interactions are trapped, and then the readout matches up each motif with the transcription factor via the barcode. (genomeweb.com)
  • Deplancke also worked in advanced bioinformatics to derive predictive models for binding interactions. (genomeweb.com)
  • Predictions of hot stops for some important protein-protein interactions are discussed. (utoronto.ca)
  • Machine learning methods provide a cost-effective and complementary approach to experimental drug bioactivity profiling, allowing for prioritization of the most potent drug candidates and protein target interactions for further verification in the laboratory. (videolectures.net)
  • This lecture will introduce the concept of kernel learning and demonstrate how kernels can be used to model drug-protein interactions. (videolectures.net)
  • Protein-protein interactions are crucial in numerous cellular functions and biological processes that take place inside our body. (lu.se)
  • Binding of serum folic acid activity (FAA) to the protein fractions during and after pregnancy was studied by DEAE Sephadex A-50 gel chromatography. (karger.com)
  • A minor proportion of the THs is bound on serum lipoproteins. (medscape.com)
  • Inherited or acquired variations in the concentration and/or affinity of these proteins may produce substantial changes in serum total TH levels measured by commercially available assays. (medscape.com)
  • More specifically, low TBG is suggested because this protein carries the majority of the serum TH. (medscape.com)
  • It interacts also with anticoagulant protein S and with serum amyloid P component. (lu.se)
  • At a constant concentration of TCPy (200 microM), levels of protein bound to TCPy in cell culture media (Advanced DMEM:F12 supplemented with 2% fetal bovine serum) decreased as much as 52% with an increasing co-exposure to 2,4-D (0-2300 microM), indicating that these chemicals compete for common binding sites on proteins. (cdc.gov)
  • Inherited thyroxine-binding globulin deficiency is a genetic condition that typically does not cause any health problems. (medlineplus.gov)
  • Researchers have identified two forms of inherited thyroxine-binding globulin deficiency: the complete form (TBG-CD), which results in a total loss of thyroxine-binding globulin, and the partial form (TBG-PD), which reduces the amount of this protein or alters its structure. (medlineplus.gov)
  • Although inherited thyroxine-binding globulin deficiency does not cause any health problems, it can be mistaken for more serious thyroid disorders (such as hypothyroidism). (medlineplus.gov)
  • Therefore, it is important to diagnose inherited thyroxine-binding globulin deficiency to avoid unnecessary treatments. (medlineplus.gov)
  • The complete form of inherited thyroxine-binding globulin deficiency, TBG-CD, affects about 1 in 15,000 newborns worldwide. (medlineplus.gov)
  • Inherited thyroxine-binding globulin deficiency results from mutations in the SERPINA7 gene. (medlineplus.gov)
  • Researchers have also described non-inherited forms of thyroxine-binding globulin deficiency, which are more common than the inherited form. (medlineplus.gov)
  • Non-inherited thyroxine-binding globulin deficiency can occur with a variety of illnesses and is a side effect of some medications. (medlineplus.gov)
  • Inherited thyroxine-binding globulin deficiency has an X-linked pattern of inheritance. (medlineplus.gov)
  • In males (who have only one X chromosome), a mutation in the only copy of the gene in each cell causes partial or complete inherited thyroxine-binding globulin deficiency. (medlineplus.gov)
  • Most of the time, these hormones circulate in the bloodstream attached to thyroxine-binding globulin and similar proteins. (medlineplus.gov)
  • Then, using the advanced robotic system in Schuldiner's lab, they systematically looked at the ability of a protein to reach the endoplasmic reticulum membrane in the absence of each gene in the yeast cell, finding three that appeared to be necessary to the process of transporting these particular "problematic" proteins. (weizmann.ac.il)
  • Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL. (harvard.edu)
  • The existence of mRNAs retaining both nuclear cap binding protein and EJC in the distal sites of neuronal processes suggests that some localized mRNAs have not yet undergone the "very first translation," which contribute to the spatio-temporal regulation of gene expression. (frontiersin.org)
  • The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. (sinica.edu.tw)
  • This gene provides instructions for making thyroxine-binding globulin. (medlineplus.gov)
  • Some mutations in the SERPINA7 gene prevent the production of a functional protein, causing TBG-CD. (medlineplus.gov)
  • In females (who have two X chromosomes), a mutation in one of the two copies of the gene in each cell reduces the amount of thyroxine-binding globulin. (medlineplus.gov)
  • Thyroxine-binding globulin (TBG) is a 395-amino acid, 54kd polypeptide that is synthesized in the liver and encoded by a single gene copy. (medscape.com)
  • The search specifically identifies the DNA of the gene that produces the ricin protein. (cdc.gov)
  • In molecular biology, the auxin binding protein family is a family of proteins which bind auxin. (wikipedia.org)
  • These RNA-binding proteins could also be used to build molecular assembly lines that would bring together enzymes needed to perform a series of reactions that produce a drug or another molecule of interest. (genengnews.com)
  • However, molecular weight is also important due to potential steric incompatibilities within protein cores. (hindawi.com)
  • The team led by Dr. Kuo-Chiang Hsia (Institute of Molecular Biology, Academia Sinica) and Dr. Wei-Yi Chen (Institute of Biochemistry and Molecular Biology, College of Life Sciences, National Yang Ming Chiao Tung University) presents a cryo-EM structure of Kap114p, one of the Kap-βs, in complex with TATA box binding protein and reveals a non-canonical function beyond nuclear transport that modulates TBP-dependent transcription. (sinica.edu.tw)
  • In order to explore the nature of interaction of these bacteria to their hosts, a molecular level study was proposed primarily to isolate and characterize surface layer and secretory proteins. (amrita.edu)
  • The ability of GAS to bind to fibronectin (Fn), a high molecular weight glycoprotein of the extracellular matrix, has long been known to be essential for the pathogen's evolutionary success and fitness. (lu.se)
  • Okamoto H, Mori Y, Tani Y, Nakagomi Y, Sano T, Ohyama K, Saito H, Oiso Y. Molecular analysis of females manifesting thyroxine-binding globulin (TBG) deficiency: selective X-chromosome inactivation responsible for the difference between phenotype and genotype in TBG-deficient females. (medlineplus.gov)
  • In the ideal scenario, we would be able to attribute the contributions from individual molecular groups to the enthalpy and entropy of binding. (lu.se)
  • In the first part of this thesis, the interaction between the rat CD2 (rCD2) adhesion protein and its ligand rat CD48T92A (rCD48T92A), a high-affinity mutant of the wild type rat CD48, was used to establish a new method of obtaining single-cell binding affinities of T cells interacting with SLBs using imidazole titrations. (lu.se)
  • When both proteins find their target sequence, the GFP molecules join and become fluorescent-a signal to the researchers that the target RNA is present. (genengnews.com)
  • Recognizing similarities and deriving relationships among protein molecules is a fundamental requirement in present-day biology. (nih.gov)
  • Inferring functional similarities between protein molecules by comparing their binding sites is still largely exploratory and not as yet a routine protocol. (nih.gov)
  • These programs are utilized to screen entire libraries of molecules for a possible ligand of a binding site and to furthermore estimate in which conformation the ligand will most likely bind. (uni-marburg.de)
  • Each protein is also attached to half of a green fluorescent protein (GFP) molecule. (genengnews.com)
  • Furthermore, the team discovered that each time an mRNA molecule is translated, the GFP gets knocked off, and when translation is finished, another GFP binds to it, enhancing the overall fluorescent signal. (genengnews.com)
  • In this thesis, total internal fluorescence microscopy (TIRF) and single-molecule imaging were used to observe and characterize protein-functionalized supported lipid bilayers (SLBs) interacting with immune cells to obtain the binding kinetics of various protein-protein pairs. (lu.se)
  • For this reason, the influence of an adhesion molecule, rat CD2, on the obtained binding kinetics of the human CD4 co-receptor was initially examined showing that the accumulation of CD4 was influenced when having a high concentration of bound CD2 inside the cell-SLB contacts. (lu.se)
  • In this study, using direct visualization of single actin filament elongation, we show that actin polymerization kinetics at both filament ends are strongly influenced by the binding of proteins to the lateral filament surface. (cipsm.de)
  • When these genes were missing, the researchers observed accumulations of protein within the cell - proteins that had not managed to reach their destination outside of it. (weizmann.ac.il)
  • The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
  • and positive regulation of protein phosphorylation. (nih.gov)
  • Protein phosphorylation plays an important role in physiological processes, such as muscle contraction. (scirp.org)
  • Phosphorylation of cMyBP-C is essential for normal cardiac function, since dephosphorylation of this protein leads to its degradation and has been associated with cardiomyopathy. (scirp.org)
  • Cohen, P. (2002) The origins of protein phosphorylation. (scirp.org)
  • Barefield, D. and Sadayappan, S. (2010) Phosphorylation and function of cardiac myosin-binding protein c in health and disease. (scirp.org)
  • High- and low-resolution structures of the MORN1 protein from Trypanosoma brucei and homologous proteins from the parasites Toxoplasma gondii and Plasmodium falciparum were obtained using a combination of macromolecular crystallography, small-angle X-ray scattering, and electron microscopy. (biorxiv.org)
  • Hoping to make the assembly of RNA-binding proteins less of a trial-and-error and more programmable task, the MIT researchers tested many amino acid combinations. (genengnews.com)
  • Ultimately, the researchers found a particular set of amino acids that will bind each of the four bases at any position in the target sequence. (genengnews.com)
  • To achieve that, the researchers attached a protein called a translation initiator to the Pumby protein. (genengnews.com)
  • To begin, the researchers identified proteins that need to pass through the endoplasmic reticulum transit area, but do not receive assistance from either known pathway. (weizmann.ac.il)
  • Researchers from Case Western Reserve University School of Medicine have found unexpected similarities between proteins that were thought to be fundamentally different. (labmanager.com)
  • In a paper published in Nature Methods this week, a Switzerland-based team of researchers, led by bioengineer Bart Deplancke of the École Polytechnique Fédérale de Lausanne (EPFL), showcased the assay's ability to determine DNA-binding specificities for transcription factors. (genomeweb.com)
  • Researchers have provided new insight on how two proteins help influenza A virus particles fight their way to human cells. (infectioncontroltoday.com)
  • affigel 15 and peptide binding protein elution. (bio.net)
  • Dear Netters, We are interested in purifying a protein (from detergent extracted microsome preps) which binds a particular peptide. (bio.net)
  • In order to determine the specificity I perpared a 6 mM solution of the peptide in binding buffer (MES 100 mM 1 % CHAPS detergent) after binding the protein and washing the column I added 2 ml of the elution buffer (6 mM peptide in wash buffer) and the column (1 ml) swelled up to 5 ml and would not settle. (bio.net)
  • In this presentation, we will show results of our approach for the hydration of water, of a peptide model in different osmolyte mixtures, and finally for the protein cytochrome C. The potential utility of this approach for studying ion and osmolyte-specific effects in protein solution thermodynamics will be indicated. (aiche.org)
  • Even though the CD4 co-receptor increases the sensitivity of T cell signalling manyfold, it binds to its ligand, peptide major histocompatibility complex II (pMHCII), with the lowest binding affinity known to this day. (lu.se)
  • A peptide mimicking the binding site of laminin onto PrPC (Ln-γ1) binds to PrPC and induces intracellular Ca2+ increase in neurons via the complex PrPC-mGluR5. (bvsalud.org)
  • Lastly, CD4 binding studies showed that the co-receptor did not noticeably affect the TCR-MHC II binding at physiological levels of hCD4 in the SLB. (lu.se)
  • The prion protein (PrPC) has been suggested to operate as a scaffold/receptor protein in neurons , participating in both physiological and pathological associated events. (bvsalud.org)
  • Additional protein binding (at physiological conditions) and kinetic cellular transport experiments quantifying chemical transport and binding rates were conducted to further explore implications of chemical mixtures transport. (cdc.gov)
  • We will focus on quantitative binding affinity prediction in order to fully characterize the activity spectrum of a drug. (videolectures.net)
  • Estrogen receptor-alpha (ERalpha) promotes proliferation of breast cancer cells, whereas tumor suppressor protein p53 impedes proliferation of cells with genomic damage. (nih.gov)
  • To infect cells without getting stuck in the mucus, influenza A relies on a balance between two proteins on the surface of its viral particles: the receptor-binding protein hemagglutinin (HA) and the cleaving protein neuraminidase (NA). (infectioncontroltoday.com)
  • Functional maturation of steroid hormone receptors requires ordered assembly into a large multichaperone complex consisting of receptor monomer, an Hsp90 dimer, the p23 cochaperone, and an FK506-binding protein (FKBP) family member or alternate peptidylprolyl isomerase-related cochaperone. (unboundmedicine.com)
  • Protein binding site comparisons are frequently used receptor-based techniques in early stages of the drug development process. (uni-marburg.de)
  • The results showed a relatively small spread in the rCD2-rCD48T92A binding affinity values despite the considerable spread of receptor densities within the cell population. (lu.se)
  • PrPC, laminin , and metabotropic glutamate receptor 5 (mGluR5) form a protein complex on the plasma membrane that can trigger signaling pathways involved in neuronal differentiation. (bvsalud.org)
  • To design the protein, they first identified hot-spots of interaction on the virus protein. (thenakedscientists.com)
  • In this talk we develop an efficient approach (Interaction Entropy) to computing quantitative residue-specific contributions to protein-protein and protein-ligand binding free energies. (utoronto.ca)
  • Studies for an extensive set of realistic protein-protein interaction systems and for specific protein-ligand binding systems showed that by including the entropic contribution, the computed residue-specific binding free energies are in better agreement with the corresponding experimental data. (utoronto.ca)
  • 1. Duan, L.L., X. Liu, and J.Z.H. Zhang, Interaction Entropy: A New Paradigm for Highly Efficient and Reliable Computation of Protein-Ligand Binding Free Energy. (utoronto.ca)
  • Zhang, J.Z.H., Interaction entropy for protein-protein binding. (utoronto.ca)
  • Computational Alanine Scanning with Interaction Entropy for Protein-Ligand Binding Free Energies, J. Chem. (utoronto.ca)
  • Through this interaction, mRNAs and their associative proteins form messenger ribonucleoprotein particles (mRNPs) that are actively transported along the cytoskeleton to intracellular destinations. (frontiersin.org)
  • Understanding how proteins interact entails studying the binding affinity (strength) and the lifetime (duration) of the protein-protein interaction to better decompose how an immune response is initiated and how we can explore this knowledge to treat diseases. (lu.se)
  • For most of these proteins, getting to the outside of the cell involves passage through an organelle called the endoplasmic reticulum. (weizmann.ac.il)
  • The first pathway for transferring proteins into the endoplasmic reticulum was discovered in the 1980s," says Schuldiner. (weizmann.ac.il)
  • But this finding was not the whole story: Although many proteins use the SRP pathway to get to the endoplasmic reticulum membrane, this shuttle system seemed to have trouble identifying other outward-bound proteins. (weizmann.ac.il)
  • These are proteins that, if they are not efficiently transported to the endoplasmic reticulum, the cell dies. (weizmann.ac.il)
  • one on the ribosome (the cell's protein-manufacturing complex) and the other two at the "gates" of the endoplasmic reticulum. (weizmann.ac.il)
  • A protein involved in transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS. (umassmed.edu)
  • Candidate proteins were tested using a highly efficient yeast-based assay, and two of the designed proteins, HB36 and HB80, were shown to bind well with the 1918 H1N1, as well as others strains of H1N1 and H5N1. (thenakedscientists.com)
  • 97 Pages Report] The protein binding assay market is projected to reach USD 425.7 million in 2023 from USD 231.3 million in 2017, at CAGR of 10.7% during the forecast period. (marketsandmarkets.com)
  • Top-down and bottom-up approaches were used to validate the size of the global protein binding assay market and estimate the size of various other dependent submarkets. (marketsandmarkets.com)
  • The key players operating in the protein binding assay market are Thermo Fisher Scientific (US), GE Healthcare (US), Danaher (US), and Merck (Germany). (marketsandmarkets.com)
  • NEW YORK (GenomeWeb) - A new microfluidics-driven DNA-protein binding assay has its creators dreaming of launching a startup to cash in on several potential applications. (genomeweb.com)
  • The assay could help accelerate efforts to map where transcription factors bind the genomes of humans and model organisms, Deplancke said. (genomeweb.com)
  • Bacteria often can't support over-expression needed to get workable quantities of protein for an assay, and in vitro expression often can't synthesize a fully functional transcription factor. (genomeweb.com)
  • A membrane-bound protein, is a protein that is bound (attached) to a biological membrane, may refer to: Integral membrane protein (permanently attached or built in) Peripheral membrane protein (temporarily attached) This disambiguation page lists articles associated with the title Membrane-bound protein. (wikipedia.org)
  • This first entails getting across the membrane of this organelle, to which the proteins are targeted, with the help of a special "shuttle" that conducts them to a sort of "transit area," checking them first to see if they have the proper "passports. (weizmann.ac.il)
  • 1. Membrane-bound and free polyribosomes were isolated from parathyroid glands of normal dogs by a discontinuous density-gradient technique. (portlandpress.com)
  • 4. The results indicate that it is possible to isolate and directly study the protein synthetic activity of membrane-bound and free parathyroid ribosomes. (portlandpress.com)
  • Based on primary sequence comparisons, β subunits are predicted to be modular structures composed of five domains (A-E) that are related to the large family of membrane-associated guanylate kinase proteins. (jneurosci.org)
  • MORN (membrane occupation and recognition nexus) repeat proteins have a wide taxonomic distribution, being found in both prokaryotes and eukaryotes. (biorxiv.org)
  • In functional terms, they are often assumed to be lipid-binding modules that mediate membrane targeting, but direct evidence for this role is actually lacking. (biorxiv.org)
  • A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. (harvard.edu)
  • The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. (harvard.edu)
  • Here we report a class of mRNA granules in human neuronal processes that are enriched in the nuclear cap-binding protein complex (CBC) and exon junction complex (EJC) core components, Y14 and eIF4AIII. (frontiersin.org)
  • Most natural antibodies bind to the variable head of the protein, leaving room for the virus to evolve immunity, but the tail is highly conserved across strains, making it an excellent target for attack. (thenakedscientists.com)
  • Such antibodies (Abs) circumvent antigenic escape and efficiently bind to various M types. (lu.se)
  • Very rarely, and in the context of anti-TH antibodies in autoimmune thyroid disease, immunoglobulins also may bind TH. (medscape.com)
  • The methyl-CpG-binding protein MeCP2 was discovered over 15 years ago as part of a search for proteins that selectively bind methylated DNA. (portlandpress.com)
  • At the end of the lecture, we will also go through the summary of the results from the Illuminating the Druggable Genome (IDG)-DREAM Drug-Kinase Binding Prediction Challenge. (videolectures.net)
  • Is the Subject Area "Protein structure prediction" applicable to this article? (plos.org)
  • Matching of structural motifs using hashing on residue labels and geometric filtering for protein function prediction. (uni-marburg.de)
  • Retention within the lumen of the ER correlates with an additional signal located at the C terminus, represented by the sequence Lys-Asp-Glu-Leu, known to be responsible for preventing secretion of proteins from the lumen of the ER in eukaryotic cells. (wikipedia.org)
  • The LIM-domain binding protein, binds to the LIM domain pfam00412 of LIM homeodomain proteins which are transcriptional regulators of development. (nih.gov)
  • Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. (nih.gov)
  • Immobilized metal affinity chromatography (IMAC) resin utilizing cobalt (Co 2+ ) for the purification of 6x histidine tagged proteins. (gbiosciences.com)
  • The surface layer proteins were extracted through lithium chloride treatment followed by their isolation and purification by fibronectin and gelatin affinity chromatography. (amrita.edu)
  • The nuclear poly(A) binding protein, PABP2, forms an oligomeric particle covering the length of the poly(A) tail. (harvard.edu)
  • As TMV has a stoichiometry of three nucleotides per protein subunit, the sequence of TMV RNA suggested to be the nucleation site for self-assembly of the virus has three possible binding frames. (nature.com)
  • This protein helps the virus to invade cells, and is found in many strains of influenza. (thenakedscientists.com)
  • Mucosal barriers are the body's first line of defense against influenza A infection, containing sialic acid decoys that bind the virus. (infectioncontroltoday.com)
  • To explore this, Vahey and his colleague Daniel Fletcher, also at the University of California, Berkeley, and Faculty Scientist at the Lawrence Berkeley National Laboratory, used fluorescent labelling and super-resolution microscopy to study the organisation of HA and NA on influenza A. Their work revealed that the proteins are distributed asymmetrically in such a way that the binding and cleaving leads to persistent directional motion. (infectioncontroltoday.com)
  • The paper 'Influenza A virus surface proteins are organized to help penetrate host mucus' can be freely accessed online at https://doi.org/10.7554/eLife.43764. (infectioncontroltoday.com)
  • These bisected N -glycans and their non-bisected counterparts were used to construct a distinctive glycan microarray to study their recognition by a wide variety of glycan-binding proteins (GBPs), including plant lectins, animal lectins, and influenza A virus hemagglutinins. (rsc.org)
  • SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
  • Pocketome via Comprehensive Identification and Classification of Ligand Binding Envelopes. (uni-marburg.de)
  • Specific binding/wash and elution buffers are available. (gbiosciences.com)
  • Our new HOOK™ 6X His Protein Purification kits are now available and include everything required for purification of 6X His tagged proteins from yeast or bacteria, including lysis buffers, lytic enzymes, resin, columns and binding, wash and elution buffers. (gbiosciences.com)
  • A sub - family of RHO GTP- BINDING PROTEINS that is involved in regulating the organization of cytoskeletal filaments. (dictionary.net)
  • Sso10a is a member of a group of DNA-binding proteins thought to be important in chromatin structure and regulation in the hyperthermophilic archaeon Sulfolobus solfataricus. (rcsb.org)
  • Regulation of Amyloid ß Oligomer Binding to Neurons and Neurotoxicity by the Prion Protein-mGluR5 Complex. (bvsalud.org)
  • Enables kinase binding activity and protein serine/threonine kinase activator activity. (nih.gov)
  • One of the upstream kinases, which phosphorylate cMyBP-C, is protein kinase D (PKD). (scirp.org)
  • If there is a shortage (deficiency) of thyroxine-binding globulin, the amount of circulating thyroid hormones is reduced. (medlineplus.gov)
  • Thyroxine-binding globulin (TBG) deficiency is a benign condition that is either acquired or inherited. (medscape.com)
  • Several states of deficiency of this protein have been described that are either inherited or acquired. (medscape.com)
  • In addition to NMR relaxation methods, we use isothermal titration calorimetry to obtain the thermodynamic fingerprint of ligand binding, including the total (standard) free energy, enthalpy, and entropy of the process. (lu.se)
  • Sarel J. Fleishman at the University of Washington, along with colleagues in California, used cutting edge software and over 100,000 hours of parallel computing time to design new proteins that would bind to the tail of a common virus protein, Hemagglutinin, from the 1918 H1N1 'flu strain. (thenakedscientists.com)
  • Both proteins consist of three domains: two FKBP12-like domains termed FK1 and FK2 and a tetratricopeptide repeat domain that targets binding to Hsp90. (unboundmedicine.com)
  • the Cambridge Structural Database (CSD) and the Protein Data Bank (PDB). (rcsb.org)
  • To benefit from the enormous amount of structural data that is being rapidly accumulated, it is essential to have high throughput tools that enable large scale binding site comparison. (nih.gov)
  • The structural comparison of binding sites is especially useful when applied on distantly related proteins as a comparison solely based on the amino acid sequence is not sufficient in such cases. (uni-marburg.de)
  • In a collaborative effort, the Lund Protein Production Platform (LP3) , the FragMAX platform at the BioMAX beamline of the MAX IV laboratory, the Deuteration and Macromolecular Crystallization (DEMAX) of the European Spallation Source ESS and the group of Professor F. Kozielski at University College London published new results on non-structural protein from SARS-CoV-2. (lu.se)
  • MeCP2 is most highly expressed in neurons, and mice lacking this protein show symptoms that strikingly parallel those of Rett patients. (portlandpress.com)
  • Herein, we demonstrated that an existing complex containing PrPC-mGluR5 has an important role in AßO binding and activity in neurons . (bvsalud.org)
  • It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. (nih.gov)
  • Poly(A)-Binding Protein II" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
  • In convergent evolution, nonhomologous proteins evolve in separate biological contexts to catalyze the same or similar reactions. (hindawi.com)
  • MIR-1265 regulates cellular proliferation and apoptosis by targeting calcium binding protein 39 in gastric cancer and, thereby, impairing oncogenic autophagy. (nih.gov)
  • Recently, especially kernel-based methods have received significant attention in pharmacology offering, among others, the advantage of computationally efficient modelling of the nonlinearities between chemical and protein features and drug bioactivity profiles. (videolectures.net)
  • However, some strains lack the ability to efficiently bind Fn. (lu.se)
  • We, moreover, saw that our Abs could enhance Fn binding in 3 out of 5 emm type strains tested, belonging to different clades, making it likely that this is a more generalizable phenomenon. (lu.se)
  • By comparing NMR-derived order parameters between the free and ligand-bound protein, we estimate the contributions from conformational entropy to the free energy of ligand binding. (lu.se)
  • After intraperitoneal and oral administration of [ 14 C]HCP (5 mg/kg) to male rats treated with phenobarbital, radiocarbon was covalently bound mainly to proteins of the liver, plasma, and kidney. (aspetjournals.org)
  • Covalently bound radioactivity in the liver was associated primarily with proteins in the microsomal and 105,000 x g supernatant fractions. (aspetjournals.org)
  • In terms of predicting where in the genome the transcription factors bind, the models we're able to derive seem to be, in general, actually better than the other assays. (genomeweb.com)
  • The 95 amino acid residue protein contains a winged helix DNA-binding domain with an extended C-terminal alpha-helix that leads to dimerization by forming a two-stranded, antiparallel coiled-coil rod. (rcsb.org)
  • Nature's design of proteins (Why was that metal or amino acid selected? (lu.se)
  • In some cases similarities obscure at these levels could be present merely in the substructures at their binding sites. (nih.gov)
  • Addgene: Expression cloning of a cDNA encoding a retinoblastoma-binding protein with E2F-like properties. (addgene.org)