• Fundamental Study of the Structure of the Glycerol-3-Phosphate Acyltransferase (GPAT) Protein from Chlorella Sp. (iium.edu.my)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) in Tissue homogenates, cell lysates and other biological fluids. (noveoninc.com)
  • Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. (noveoninc.com)
  • Description: A sandwich ELISA kit for detection of Glycerol-3-Phosphate Acyltransferase, Mitochondrial from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids. (noveoninc.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Rat Glycerol-3-Phosphate Acyltransferase, Mitochondrial (GPAM) in samples from tissue homogenates or other biological fluids. (noveoninc.com)
  • 1992). The NS2B can be an essential membrane protein in the endoplasmic reticulum (Clum et al. (findadig.com)
  • Here, we show that mouse Stbd1 is a transmembrane endoplasmic reticulum (ER)-resident protein with the capacity to induce the formation of organized ER structures in HeLa cells. (imperial.ac.uk)
  • PORCN selectively catalyzes the palmitoylation of Wnt proteins in the endoplasmic reticulum, a process which is essential for Wnt secretion and thus the induction of Wnt signaling. (tocris.com)
  • I am a plant cell biologist and protein biochemist at Oxford Brookes University with expertise in the structure and function of the plant endoplasmic reticulum (ER), membrane proteins and auxin biosynthesis using biochemical techniques as well as high-resolution live cell imaging. (brookes.ac.uk)
  • The synthesis of E, M, and S proteins occurs in the ribosomes associated with the endoplasmic reticulum (ER). (news-medical.net)
  • This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. (wikipedia.org)
  • The systematic name of this enzyme class is acyl-[acyl-carrier protein]:O-(2-acyl-sn-glycero-3-phospho)ethanolamine O-acyltransferase. (wikipedia.org)
  • Lecithin-cholesterol acyltransferase (LCAT) is a lipoprotein-associated enzyme which plays a large role in the esterification of free cholesterol, the maturation of high density-lipoprotein (HDL) particles, and the intravascular stage of reverse cholesterol transport (RCT). (medscape.com)
  • The ABHD5 gene provides instructions for making a protein that turns on (activates) an enzyme called adipose triglyceride lipase (ATGL). (medlineplus.gov)
  • The ABHD5 protein and the ATGL enzyme are found on the surface of lipid droplets. (medlineplus.gov)
  • lux E makes the proteins needed for the enzyme acyl-protein synthetase. (igem.org)
  • The extracellular signaling protein Sonic hedgehog (Shh) requires dual lipidation for functional signaling, whereby N-terminal palmitoylation is performed by the enzyme Hedgehog acyltransferase (Hhat). (imperial.ac.uk)
  • Function in phospholipid biosynthesis and have either glycerolphosphate, 1-acylglycerolphosphate, or 2-acylglycerolphosphoethanolamine acyltransferase activities. (embl-heidelberg.de)
  • My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)
  • Yeast microsomes expressing EaDAcT possessed acetyl-CoA diacylglycerol acetyltransferase activity but lacked long-chain acyl-CoA diacylglycerol acyltransferase activity. (nih.gov)
  • Functional annotation resulted in the identification of almost all genes involved in diverse lipid-metabolic pathways, including the novel fatty acid desaturase/epoxygenase, diacylglycerol acyltransferase 1 and 2, and phospholipid:diacylglycerol acyltransferases. (researchsquare.com)
  • The purpose of the present study was to test the hypothesis that lecithin:cholesterol acyltransferase (LCAT) deficiency would accelerate atherosclerosis development in low density lipoprotein (LDL) receptor (LDLr-/-) and apoE (apoE-/-) knockout mice. (eurekamag.com)
  • Apo-A1 is produced in the liver and intestines and activates lecithin-cholesterol acyltransferase (LCAT) in the peripheral tissues, which transforms free cholesterol to cholesterol ester and facilitates its transportation to the liver, were it is degraded. (medscape.com)
  • Porcupine (PORCN) is a membrane bound O-acyltransferase (MBOAT) that is involved in the acylation (palmitoylation) of Wnt proteins. (tocris.com)
  • Interestingly PORCN has been shown to be the first acyltransferase that is itself palmitoylated. (tocris.com)
  • Other names in common use include acyl-[acyl-carrier, protein]:O-(2-acyl-sn-glycero-3-phospho)-ethanolamine, and O-acyltransferase. (wikipedia.org)
  • 3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-3-O-((3R)- hydroxytetradecanoyl)-alpha-D-glucosamine = UDP-2-N,3-O-bis((3R)-3- hydroxytetradecanoyl)-alpha-D-glucosamine + a holo-[acyl-carrier- protein]. (cathdb.info)
  • LpxD from Escherichia coli prefers (R,S)-3-hydroxymyristoyl-[acyl- carrier protein] over (R,S)-3-hydroxypalmitoyl-[acyl-carrier protein]. (cathdb.info)
  • After 16 weeks of atherogenic diet (0.1% cholesterol, 10% calories from palm oil) consumption, LDLr-/- LCAT-/- double knockout mice, compared with LDLr-/- mice, had similar plasma concentrations of free (FC), esterified (EC), and apoB lipoprotein cholesterol, increased plasma concentrations of phospholipid and triglyceride, decreased HDL cholesterol, and 2-fold more aortic FC (142 +/- 28 versus 61 +/- 20 mg/g protein) and EC (102 +/- 27 versus 61+/- 27 mg/g). (eurekamag.com)
  • We have previously identified NFAT (nuclear factor of activated T-cells) as a key regulator of insulin gene transcription in pancreatic β-cells that is activated by the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin) in response to increased [Ca 2+ ] i ( 10 ). (diabetesjournals.org)
  • The protein encoded by this gene belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. (thermofisher.com)
  • Our lab focuses on investigating the structure, function, and regulation of proteins involved in cell signaling, epigenetics, and gene regulation. (bu.edu)
  • These results demonstrate the utility of deep transcriptional profiling with multiple tissues as a gene discovery strategy for low-abundance proteins. (nih.gov)
  • The roles of b-catenin are 'classically' defined: as an adhesion protein and as a signaling protein, transducing extracellular signals to the nucleus to modify gene expression. (chemdiv.com)
  • from loquat fruitlets and used to study the characteristics of different proteins. (researchensemble.com)
  • This library represents a selection of drug-like compounds aimed at modulating protein-protein interaction (PPI) of β-catenin with different proteins involved in significant physiological processes. (chemdiv.com)
  • However, peptide aggregation in vivo typically occurs in a more complex environment, surrounded by either different proteins or lipids, or both. (lu.se)
  • Phospholipid/glycerol acyltransferase is not found in the viruses or the archaea and is under represented in the bacteria. (embl-heidelberg.de)
  • An uncharacterized membrane-bound O-acyltransferase (MBOAT) family member was the most abundant acyltransferase in the endosperm but was absent from the aril. (nih.gov)
  • RNA genome can be translated to an individual polyprotein precursor which can be processed to create 10 adult proteins by co- and post-translational digesting (Rice and Lindenbach, 2003). (findadig.com)
  • Post-translational modifications (PTMs) are important modulators of biology and inhibition of protein lipidation has emerged as a promising therapeutic strategy for treatment of parasitic diseases. (imperial.ac.uk)
  • We use chemical, semi-synthetic, biophysical, and cellular approaches to elucidate mechanisms for how post-translational modifications regulate protein function. (bu.edu)
  • Palmitoylation is a post-translational modification where fatty acids, such as palmitic acid, are transferred to target proteins. (tocris.com)
  • In addition it can also accept monoamine substrates to catalyze post-translational modifications (e.g., protein serotonylation). (bvsalud.org)
  • 2. Methods and Materials 2.1 Components The DENV2 NS2B-NS3pro expression plasmid encoding the protease precursor found in this research provides the hydrophilic domains of NS2B cofactor (48 proteins) as well as the NS3pro domains (185 residues) (Yon et al. (findadig.com)
  • Protein Expression and Purif. (bu.edu)
  • Expression, purification, and characterization of mouse glycine N -acyltransferase (mGLYAT) in Escherichia coli . (bu.edu)
  • Age-dependent association between protein expression of the embryonic stem cell marker Cripto-1 and survival of glioblastoma patients. (uib.no)
  • Protein Expression and Purification. (lu.se)
  • Identification of an arylalkylamine N -acyltransferase from Drosophila melanogaster that catalyzes the formation of long-chain N -acylserotonins. (bu.edu)
  • Calcium-dependent acyltransferase that catalyzes cross-linking of proteins at a GLUTAMINE in one chain with primary amine such as in LYSINE in another chain. (bvsalud.org)
  • Scientists have reported that in viruses such as SARS-CoV-1 and mouse hepatitis virus (MHV), both S and E proteins undergo S-acylation. (news-medical.net)
  • S-acylation is regarded as a hallmark of viral envelope proteins. (news-medical.net)
  • It has also characterized Spike acylation and has also identified other acylated viral proteins and host enzymes involved in the process of infection. (news-medical.net)
  • In this study, researchers have used two assays to monitor protein acylation. (news-medical.net)
  • Further, this research has also shown that following synthesis, acylation of the S protein takes place in the ER by ZDHHC20. (news-medical.net)
  • Researchers have also studied how S-acylation modifies the turnover rate of proteins. (news-medical.net)
  • E. coli-derived human Abhd5 recombinant protein (Position: R169-D349). (thermofisher.com)
  • Additionally, they found a rapid degradation of spike protein following its synthesis when ZDHHC enzymes were silenced. (news-medical.net)
  • Our chemical total syntheses of these Abies sesquiterpenoids have enabled (i) the validation of beshanzuenone C's weak PTP1B inhibiting potency, (ii) identification of new synthetic analogs with promising and selective protein tyrosine phosphatase SHP2 inhibiting potency, and (iii) preparation of azide-tagged probe molecules for target identification via a chemoproteomic approach. (acs.org)
  • Mechanistic analysis of ghrelin- O -acyltransferase using substrate analogs. (bu.edu)
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
  • CoVs are enveloped by a protein membrane that consists of a minimum of three structural proteins, namely, Spike (S), Membrane (M), and Envelope (E). They also contain accessory factors such as Orf3a. (news-medical.net)
  • It really is proposed that DHNs can defend proteins and membranes from unfavorable structural adjustments due to dehydration. (researchensemble.com)
  • Apolipoprotein A-I (Apo-A1) is a structural and functional protein that constitutes approximately 70% of the protein in high density lipoprotein (HDL). (medscape.com)
  • The response to GLP-1 was mimicked by forskolin and largely inhibited by the protein kinase A (PKA) inhibitors, H89 and myristoylated PKI(14-22) amide, indicating partial mediation via a cAMP/PKA pathway. (diabetesjournals.org)
  • The serine/threonine kinase protein kinase D1 (PKD1) is a stress-responsive kinase and sensor for reactive oxygen species, which can initiate cell survival through NF-κB signaling. (biologists.com)
  • Cloning and characterization of murine 1-acyl-sn-glycerol 3-phosphate acyltransferases and their regulation by PPARalpha in murine heart. (nih.gov)
  • These DHN proteins are all highly hydrophilic, but they differ significantly in size, ranging from 188 to 475 amino acids, and in biochemical properties, such as theoretical pI, aliphatic index, and instability index. (researchensemble.com)
  • However, vegetation use multiple mechanisms to increase their tolerance to freezing temps, such as accumulation of compatible osmolytes (soluble sugars, glycine betaine, and proline) and improved levels of antioxidants and soluble proteins in cell cytoplasm [2]C[3]. (researchensemble.com)
  • The shortest forms, lacking the hydrophobic stretch, may be soluble cytoplasmic proteins. (lu.se)
  • Among these, DHNs, also called LEA II (late embryogenesis abundant) proteins have been evaluated. (researchensemble.com)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • Artemisinin inhibits NRas palmitoylation by targeting the protein acyltransferase ZDHHC6. (mpg.de)
  • Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. (thermofisher.com)
  • Starch binding domain-containing protein 1 (Stbd1) is a carbohydrate-binding protein that has been proposed to be a selective autophagy receptor for glycogen. (imperial.ac.uk)
  • In this review we summarize the latest insights into protein lipidation in protozoan parasites. (imperial.ac.uk)
  • We discuss how recent chemical proteomic approaches have delivered the first global overviews of protein lipidation in these organisms, contributing to our understanding of the role of this PTM in critical metabolic and cellular functions. (imperial.ac.uk)
  • Thereby, the researchers have identified ZDHHC20 as the key acyltransferase responsible for the lipidation of SARS-CoV-2 Spike, E, and Orf3a. (news-medical.net)
  • E and M proteins are essential for viral assembly, whereas, S protein mediates the attachment of the virus to the host cell receptors, and also takes part in the transfer of the viral genome to the host. (news-medical.net)
  • These proteins were firstly discovered in 1989. (chemdiv.com)
  • Every proteins in this family Enzastaurin distributor members includes at least one duplicate of a lysine-wealthy amino acid sequence known as the K-segment, that is generally located close to the carboxyl terminus. (researchensemble.com)
  • A multifunctional role of Atg8-family proteins (Atg8 from yeast and human LC3 and GABARAP subfamilies, all referred to here as ATG8s) in macroautophagy/autophagy is carried out by two protein domains, the N-terminal helical domain (NHD) and ubiquitin-like (UBL) domain. (bvsalud.org)
  • The viral protease cleavage sites have in common a set of basic proteins, K and R, accompanied by G, S, or A on the P1 placement (Lindenbach and Grain, 2003). (findadig.com)
  • I took up a position at Oxford Brookes University in 2012 investigating the role of reticulon proteins in ER tubulation and viral trafficking in order to develop my international reputation in ER research and advanced imaging. (brookes.ac.uk)
  • I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
  • Orthologous to human AGPAT5 (1-acylglycerol-3-phosphate O-acyltransferase 5). (nih.gov)
  • The mission of our lab is to understand the basic biological mechanisms of proteins that are essential to human health and disease. (bu.edu)
  • Goat Anti-Mouse IgG 2a -HRP antibody with minimal reactivity to human proteins for use in ELISA, immunohistochemistry / immunocytochemistry, western blot, and ELISpot assays. (southernbiotech.com)
  • A truncated C-terminal fragment of Mycobacterium tuberculosis HSP70 enhances cell-mediated immune response and longevity of the total IgG to influenza A virus M2e protein in mice. (southernbiotech.com)
  • Here I pursued my scientific interests in subcellular protein localisation, bioinformatics, and mathematical modelling. (brookes.ac.uk)
  • Beshanzuenones C ( 2 ) and D ( 3 ) are isolated from the critically endangered Chinese fir tree species Abies beshanzuensis and demonstrated weak inhibiting activity against protein tyrosine phosphatase 1B (PTP1B). (acs.org)
  • Recent studies have demonstrated that ATP-binding cassette protein A1 (ABCA1) is highly regulated in macrophages and mediates the efflux of cholesterol and phospholipids to apolipoproteins, a process necessary for HDL formation. (jci.org)
  • Protein Expr Purif, 172, pp. 105637. (unipv.it)
  • Probing the chemical mechanism and critical regulatory residues of Drosophila melanogaster arylalkylamine N -acyltransferase like 2. (bu.edu)