Protein activators b cell specific activator protein. Medical search. Frequent questions

It is involved in mediating the transcriptional activity of these proteins. (nih.gov)
This resulted in the hormone-dependent transcriptional activity of octamer-dependent reporters in these cells. (nih.gov)
Thus, these results demonstrate that BOB.1 / OBF.1 itself is a non-redundant protein in B cells and absolutely required for octamer-dependent transcriptional activity. (nih.gov)
Comparative functional multiomics showed that blood proteins induce distinct receptor-mediated transcriptional programs in microglia and macrophages, such as redox, type I interferon and lymphocyte recruitment. (nature.com)
We report a blood-induced microglia gene network and show that blood proteins elicit distinct receptor-mediated transcriptional changes and signaling programs in innate immune cells. (nature.com)
Impossible Foods has filed a patent for materials and methods that involve overexpression of a transcriptional activator called retrograde regulation protein 1 (Rtg1) to increase the expression of one or more polypeptides. (just-drinks.com)
In addition to the Rtg and polypeptide encoding nucleic acids, the yeast cell can also contain a third exogenous nucleic acid encoding a transcriptional activator, such as Mxr1, Mit1, or Trm1. (just-drinks.com)
This transcriptional activator is operably linked to one of the promoter elements. (just-drinks.com)
Furthermore, the yeast cell can be modified to express multiple transcriptional activators, each linked to a different promoter element. (just-drinks.com)
The use of different promoter elements, transcriptional activators, and heme biosynthesis enzymes allows for precise control and optimization of protein expression in yeast cells. (just-drinks.com)

tPA can be manufactured using recombinant biotechnology techniques, producing types of recombinant tissue plasminogen activator (rtPA) such as alteplase, reteplase, and tenecteplase. (wikipedia.org)
The concentration of our recombinant proteins is measured using the absorbance at 280nm. (antibodies-online.com)
tPA produced by such means are referred to as recombinant tissue plasminogen activator ( rtPA ). (wikidoc.org)

We first genetically engineered proteasome activators involved in either non-ubiquitinated protein degradation pathways (PA28ƒ×) or subunits of PA700, the 26S proteasome ubiquitinated pathway (S5a) into transducible lentiviral vectors. (science20.com)
These remarkable results demonstrate for the first time that it is possible to intervene therapeutically in the proteolytic pathways and organelles that participate in the specific degradation of misfolded and abnormal proteins. (science20.com)
Signaling pathways and cell mechanics involved in wound closure by epithelial cell sheets. (cytoskeleton.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)

Following compromise of the UPS, the overexpression of proteasome activator PA28 can specifically recover proteasome function and improve cell viability in both HD model and patient cells. (science20.com)
Following compromise of the UPS, overexpression of the proteasome activator subunit PA28γ, but not subunit S5a, recovered proteasome function in the HD cells. (science20.com)

For these studies we use neurons that are maintained in cell culture as well as genetically-modified strains of mice that are at increased risk for developing neurodegenerative conditions analogous to those observed in humans. (uml.edu)
A recently filed patent (Publication Number: US20230193338A1) describes a yeast cell that has been genetically modified to express specific proteins. (just-drinks.com)
The patent also describes methods for expressing the polypeptides using the genetically modified yeast cells. (just-drinks.com)
Overall, this patent presents a novel approach to genetically modifying yeast cells for the expression of specific proteins. (just-drinks.com)

Marengo's Selective T Cell Activation Repertoire (STAR) platform is a multi-specific fusion protein library that targets specific TCR Vβ variants fused to different co-stimulatory moieties to develop potent T cell activators. (labiotech.eu)

Specific rtPAs include alteplase , reteplase , and tenecteplase . (wikidoc.org)

In Huntington's disease (HD) the disease provoking mutation in the huntingtin gene eventually causes the cell to build up intranuclear and cellular inclusions of protein-aggregates, made up primarily of huntingtin. (science20.com)
Huntington's disease (HD) is a severe autosomal-dominant neurodegenerative disorder caused by a mutation within a gene, encoding huntingtin protein. (frontiersin.org)
At the molecular level, HD occurs due to an increase in the number of CAG repeats in the first exon of the gene encoding the huntingtin protein. (frontiersin.org)
More recently, his group invented tools for cleavage-free large gene insertion via mining microbial recombination protein, and developed single-cell tracking approach for studying cancer biology and cancer immunology. (stanford.edu)
What we were able to show is that the epigenetic activator Ash1is recruited to a target gene through cell-type specific non-coding RNA" he said. (biologynews.net)
The paper examined how the activator Ash1 binds to target DNA elements, known as Trithorax-reponse elements (TREs), located in the gene Ultrabithorax (Ubx). (biologynews.net)
Proven by the use of apoptosis-resistant cellular models and autophagic assays, such isoquinoline alkaloid-induced cytotoxic effect involves energy- and autophagy-related gene 7 (Atg7)-dependent autophagy that resulted from direct activation of AMP activated protein kinase (AMPK). (oncotarget.com)

Our data provide an interactive resource for investigation of the immunology of blood proteins that could support therapeutic targeting of microglia activation by immune and vascular signals. (nature.com)
Moreover, our study provides a resource for the investigation of the immunology of blood proteins in inflammatory, autoimmune and neurodegenerative diseases. (nature.com)

Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world's largest industries. (just-drinks.com)

The patent claim describes a yeast cell comprising exogenous nucleic acids encoding Rtg and a polypeptide, operably linked to promoter elements. (just-drinks.com)
Inducible promoter elements, such as methanol-inducible promoter elements, can be used to regulate the expression of the proteins. (just-drinks.com)

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antibodies-online.com)
In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antibodies-online.com)
Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antibodies-online.com)

In conclusion, isoquinoline alkaloid is a new class of compound that induce autophagic cell death in drug-resistant fibroblasts or cancers by exhibiting its direct activation on AMPK. (oncotarget.com)

From these results, we hypothesized that enhancing UPS function would improve catalytic degradation of abnormal proteins in HD. (science20.com)
We determined how to enhance UPS function and influence catalytic protein degradation and cell survival in HD. (science20.com)
This process involves the lysosomal degradation of cellular components such as misfolded proteins or damaged organelles. (oncotarget.com)

Rho GTPases mediate the regulation of cochlear outer hair cell motility by acetylcholine. (cytoskeleton.com)
Widespread domain present in Gyp6 and Gyp7, thereby giving rise to the notion that it performs a GTP-activator activity on Rab-like GTPases. (embl.de)

In Huntington's disease (HD) brains and other tissues, UPS activity is inhibited and intraneuronal nuclear protein aggregates of mutant huntingtin in HD brains indicate dysfunction of the UPS. (science20.com)

The yeast cell contains two exogenous nucleic acids, one encoding a retrograde regulation protein (Rtg) and the other encoding a polypeptide. (just-drinks.com)

The paper explains how proteins, known as epigenetic activators (such as Ash1 from the fruit fly Drosophila), bind to their target DNA and activate genes that determine what function a cell will have in the body. (biologynews.net)

We determined how this intervention altered cell survival after exposure to toxins known to simulate pathological mechanisms in HD. (science20.com)

To address the therapeutic hypothesis experimentally, we transduced UPS subunits into HD skin fibroblasts or HD mutant protein expressing striatum-derived neurons. (science20.com)
Proteasome activators involved in either the ubiquitinated or the non-ubiquitinated proteolysis were overexpressed in HD patients' skin fibroblasts or mutant huntingtin-expressing striatal neurons. (science20.com)
PA28γ also improved cell viability in mutant huntingtin-expressing striatal neurons exposed to pathological stressors, such as the excitotoxin quinolinic acid and the reversible proteasome inhibitor MG132. (science20.com)
Here we have used the induced pluripotent stem cell technology to produce patient-specific terminally differentiated GABA-ergic medium spiny neurons modeling a juvenile form of HD (HD76). (frontiersin.org)
Here we have also observed greater expression of huntingtin and an activator of store-operated calcium channels STIM2 in HD76 neurons. (frontiersin.org)
The most vulnerable cells in HD are the striatal medium spiny neurons ( Vonsattel and DiFiglia, 1998 ). (frontiersin.org)

Adaptive immune mechanisms recognize and neutralize foreign molecules or microorganisms in a specific manner. (lu.se)

We confirmed that isoquinoline alkaloid is structurally varied from the existing direct AMPK activators. (oncotarget.com)

This effect is probably due to the increase in GAP activity, which can be observed in a lysate from cells overexpressing Gyp1p. (embl.de)

Innate immune cells integrate environmental signals to rapidly activate target genes and perform specialized cellular functions 5 . (nature.com)
One cellular organelle with a central role of clearing such protein build up in the cell is the ubiquitin proteasome system (UPS). (science20.com)
The manuscript shows that cellular changes due to expression of huntingtin protein with longer CAG repeats can reduce the ubiquitin proteasome system (UPS) function in Huntington¡¦s disease cells. (science20.com)
HIV persistence may arise from ongoing residual virus replication and/or from latently-infected cells defined as the cellular reservoir in which long-lived resting memory CD4+ T cells harbouring an integrated but transcriptionally silent provirus represent the largest pool in the blood (Chomont et al. (europa.eu)
AMP activated protein kinase (AMPK), maintains normal energy balance by regulating cellular metabolisms in an AMP/ADP ratio-dependent manner, is responsible for the proper mechanistic modulation of autophagy [ 2 ]. (oncotarget.com)

The yeast cell can have the exogenous nucleic acids stably integrated into its genome or expressed from a replication-competent plasmid. (just-drinks.com)
The yeast cell can also contain a fourth exogenous nucleic acid encoding heme biosynthesis enzymes, which are involved in the production of heme, an important molecule in various biological processes. (just-drinks.com)
The yeast cells are cultured under conditions suitable for the expression of the exogenous nucleic acids. (just-drinks.com)
A yeast open reading frame sharing homology with the two known yeast Rab GTPase-activating proteins (GAPs), Gyp6p and Gyp7p, was found in a data base search. (embl.de)
Gyp1p also stimulated the GTPase activity of several other yeast Rab proteins including Ypt1p, Ypt7p, and Ypt51p but showed no GAP activity on Ypt6p and Ypt32p. (embl.de)
The finding that yeast Rab GAPs share homology with proteins in other species, such as Caenorhabditis elegans and human, suggests the existence of a conserved Rab GAP family. (embl.de)

The BOB.1 / OBF.1 / OCA-B protein (henceforth designated as BOB.1 / OBF.1) is a B cell-specific co-activator of the Oct1 and Oct2 transcription factors. (nih.gov)

1) Antibody deficiency disorders are defects in immunoglobulin-producing B cells . (lu.se)

signal transducer and activator of. (wikigenes.org)

Cell Biol. (cytoskeleton.com)

All the above proteins belong to family S1 in the classification of peptidases [ ( PUBMED:7845208 ) ] and originate from eukaryotic species. (embl.de)
The ~200 amino acid TBC/rab GTPase-activating protein (GAP) domain is well conserved across species and has been found in a wide range of different proteins from plant adhesion molecules to mammalian oncogenes. (embl.de)

However, how microglia integrate extracellular signals at sites of cerebrovascular damage and the specificity of blood proteins controlling innate immune cell polarization in disease remain poorly understood. (nature.com)
To discover the molecular programs controlling microglial and macrophage polarization by blood proteins, we developed an unbiased blood-innate immunity multiomic and genetic loss-of-function pipeline consisting of deep sequencing of blood-induced transcriptomes, functional single-cell and oxidative stress transcriptomics, global phosphoproteomics and integration with innate immune signatures from AD and MS models (Extended Data Fig. 1 ). (nature.com)
Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells. (cytoskeleton.com)

Blood protein extravasation through a disrupted blood-brain barrier and innate immune activation are hallmarks of neurological diseases and emerging therapeutic targets. (nature.com)
In my laboratory researchers are examining the roles played by axonal cytoskeletal proteins in nervous system development, and in the etiology of Alzheimer's and other related neurodegenerative diseases. (uml.edu)

Tissue-type plasminogen activator, short name tPA, is a protein that facilitates the breakdown of blood clots. (wikipedia.org)
Tissue plasminogen activator (abbreviated tPA or PLAT ) is a protein involved in the breakdown of blood clots . (wikidoc.org)

Marengo's foundational discovery of activation of T cell subsets via TCR Vβ is unprecedented and highly differentiated from the current immuno-oncology technologies we have seen," said Howard Mayer, executive vice president and head of R&D at Ipsen. (labiotech.eu)
Marengo brings a precision medicine approach to the field of Immuno-oncology with a focus on T cell activation and this collaboration is an important validation of our STAR platform beyond our lead candidate. (labiotech.eu)

Resistance of cancer cells towards apoptosis is the major cause of these symptomatic behaviours. (oncotarget.com)

However, how blood proteins polarize innate immune cells remains largely unknown. (nature.com)
2) T cell deficiencies affect the function in killing infected cells or helping other immune cells. (lu.se)

Researchers at the University of California, Riverside have discovered a molecular mechanism directing the fate and function of cells during animal development. (biologynews.net)

Cdc42 protein: GST tagged: human dominant negative (Cat. (cytoskeleton.com)
Cdc42 protein: His tagged: human constitutively active (Cat. (cytoskeleton.com)
Cdc42 protein: His tagged: human wild type (Cat. (cytoskeleton.com)
Human RASA3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antibodies-online.com)
The reconstituted cells were then washed and stained with BD Horizon™ BUV395 Mouse Anti-Human CD4 antibody (Cat. (bdbiosciences.com)

To show this, a hormone-regulated conditional allele of BOB.1 / OBF.1 was introduced into the BOB.1 / OBF.1-deficient B cells. (nih.gov)

These results demonstrate the specific functional enhancements of the UPS that can provide neuroprotection in HD cells. (science20.com)
The feature of this platform is to fine-tune the T cell response in selected T cell subsets to generate endogenous, highly functional, cancer-killing T cells for solid tumors. (labiotech.eu)

The pathological hallmark of HD is the intraneuronal nuclear protein aggregates of mutant huntingtin. (science20.com)

It is a serine protease (EC 3.4.21.68) found on endothelial cells lining the blood vessels. (wikipedia.org)
Untreated (dashed line histogram) or treated (solid line histogram) peripheral blood mononuclear cells (PBMC) from the BD Phosflow™ T Cell Kit Lyophilized Cells (Catalog No. 560760) were reconstituted in BD Pharmingen™ Stain Buffer (Cat. (bdbiosciences.com)

The fact that these epigenetic activators, such as Ash1, turn on the expression of specific target genes has been known for some time. (biologynews.net)

T cell immunodeficiencies and severe combined immunodeficiencies (SCIDs) are marked with opportunistic infections caused by common environmental microorganisms. (lu.se)

Deficiencies are highly variable with regard to symptoms, phenotype, genotype, severity, etc, because many cells and molecules are required for both natural and adaptive immunity . (lu.se)

Cytoskeleton is the pre-eminent supplier of Rho and Ras family small G-proteins (SGPs). (cytoskeleton.com)

Induction of pre-B cells with lipopolysaccharide led to increased Oct2 levels but did not significantly increase octamer-dependent transcription in BOB.1 / OBF.1-deficient B cells. (nih.gov)

The lack of activity is a direct consequence of lack of the co-activator. (nih.gov)

Glandular (tissue) kallikreins (including EGF-binding protein types A, B, and C, NGF-gamma chain, gamma-renin, prostate specific antigen (PSA) and tonin). (embl.de)

To function, each living cell needs both to build new and to degrade old or damaged proteins. (science20.com)
The transgenic transcription of non-coding TRE RNA can change the type and function of cells. (biologynews.net)

Anatomy22

Organisms8

Diseases8

Chemicals and Drugs128

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes66

Disciplines and Occupations1

Information Science3

Transcriptional10

Recombinant3

Pathways4

Overexpression2

Genetically4

Potent1

Alteplase1

Gene7

Immunology2

World's1

Promoter2

Purification3

Induce1

Degradation3

GTPases2

Huntington's1

Regulation1

Drosophila1

Survival1

Neurons6

Recognize1

Structurally1

Lysate1

Cellular5

Yeast6

Transcription factors1

Defects1

Signal1

Biol1

Species2

Polarization3

Diseases2

Breakdown of blood2

Activation2

Apoptosis1

Immune cells2

Molecular1

Human5

Show1

Functional2

Nuclear1

Blood2

Target1

Severe1

Molecules1

Family1

Induction1

Direct1

Types1

Function2