Granulosa cellsInhibitionProliferationMiceStudied in humansTumor cellsDamageUnclearProliferativeEndothelial cellsSignificantlyProteinsCancersTissuesTherapeuticToxicityRegenerationProcessesPathwayPeripheral bloodEffectivenessSensitivityReceptorsEffectsSelectivelyExcessiveStem cellsHumanSuppressionWhite bloodResistantRegulationAgentsCycleResponseCancerDrugResearchExpressionImmuneShowNormal cellsShownTestHighAmount
Granulosa cells1
- In this study, transplantation of EVs from human induced pluripotent stem cell-derived MSCs (iPSC-MSC-EVs) resulted in significant restoration of ovarian follicle numbers, improved granulosa cell proliferation, and inhibition of apoptosis in chemotherapy-damaged granulosa cells, cultured ovaries, and in vivo ovaries in mice. (zoores.ac.cn)
Inhibition3
- Previous studies have shown that Dermal Papilla Cells (DPC) from balding hair follicles were more susceptible to go into senescence after exposure to oxidative stress and growth inhibition by androgens than DPC from non-balding hair follicles. (imedpub.com)
- Alopecia Aerate (AA), an organ-specific autoimmune disease thought to result from a collapse of HF-IP, in contrast, and shows striking defects in NK cell inhibition/ containment. (imedpub.com)
- The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates. (thermofisher.com)
Proliferation4
- In this assay, over 40 actives were screened for their ability to stimulate the proliferation of hair follicle dermal papilla cells and protect against induced cellular senescence. (imedpub.com)
- Of the promising actives, three types of activities were observed actives that stimulated cellular proliferation without an observable protection against induced senescence. (imedpub.com)
- Actives that stimulated cellular proliferation and protected against induced senescence. (imedpub.com)
- [ 13 ] The toxin binds directly to the major histocompatability complex (MHC) class II proteins on target cells, subsequently stimulating the proliferation of large numbers of T lymphocytes. (medscape.com)
Mice4
- In both mice and a Phase 1 human clinical trial, long periods of not eating significantly lowered white blood cell counts. (usc.edu)
- In mice, fasting cycles then "flipped a regenerative switch": changing the signaling pathways for hematopoietic stem cells, which are responsible for the generation of blood and immune systems, the research showed. (usc.edu)
- Following the cecal ligation and puncture (CLP) procedure, intravenous dosing of AVR-25 (10 mg/kg, 6-12 h post-CLP) alone and in combination with antibiotic imipenem protected both young adult (10-12 week old) and aged (16-18 month old) mice against polymicrobial infection, organ dysfunction, and death. (nature.com)
- This compound has been shown to selectively shield normal cells against S-phase poisons 8 tubulin poisons 9 10 a Polo-like kinase 1 inhibitor (PLK1I)12 and an Aurora kinase inhibitor.7 Most importantly oral administration of nutlin-3 protects mice against PLK1I-induced neutropenia.12 Table?1. (immune-source.com)
Studied in humans1
- In fact, animal studies show that glutamine may actually decrease tumor growth while increasing susceptibility of cancer cells to radiation and chemotherapy, though such effects have not yet been studied in humans. (peacehealth.org)
Tumor cells2
- This study investigated the role of TIGAR in DNA damage response (DDR) induced by genotoxic drugs and hypoxia in tumor cells. (nature.com)
- 5) increasing the intrinsic radioresistance of normal cells through ketone bodies but decreasing that of tumor cells by targeting glycolysis. (springer.com)
Damage15
- Due to its high antioxidant content, GSE can help prevent disease and protect against oxidative stress, tissue damage, and inflammation. (healthline.com)
- Therefore, GSE likely protected against further heart damage. (healthline.com)
- Whether the elevated TIGAR expression protects DNA damage induced by chemotherapeutic agents or hypoxia has not been explored. (nature.com)
- To define a role of TIGAR in DNA damage, TIGAR expression was knocked down with siRNA in HepG2 cells. (nature.com)
- Epirubicin, a DNA damaging anticancer agent and CoCl 2 , which was used to imitate hypoxia condition, was applied to induce DNA damage in TIGAR knockdown HepG2 cells. (nature.com)
- DNA damage in HepG2 cells after TIGAR knockdown with or without treatment of epirubicin or CoCl 2 was detected by Comet assay. (nature.com)
- In a double-blind study, Japanese researchers found that the combination of vitamin E , vitamin C, and N-acetyl cysteine (NAC) -all antioxidants-protected against chemotherapy-induced heart damage without interfering with the action of the chemotherapy. (peacehealth.org)
- Acetyl-L-carnitine in the amount of 1,000 mg three times per day for eight weeks has been shown to improve nerve damage (neuropathy) caused by the chemotherapy drug cisplatin. (peacehealth.org)
- In a double-blind trial, intravenous administration of calcium and magnesium before and after administration of oxaliplatin prevented the development of oxaliplatin-induced nerve damage. (peacehealth.org)
- However, in another double-blind trial, the same treatment regimen as in the other study did not prevent oxaliplatin-induced nerve damage. (peacehealth.org)
- While chemotherapy saves lives, it causes significant collateral damage to the immune system. (usc.edu)
- It is well established that can induce sufficient DNA damage to lead to cellular senescence, in vitro . (imedpub.com)
- Chemotherapy can significantly reduce follicle counts in ovarian tissues and damage ovarian stroma, causing endocrine disorder, reproductive dysfunction, and primary ovarian insufficiency (POI). (zoores.ac.cn)
- Nevertheless lots of the medicines currently used in the medical center cause DNA damage and indiscriminately target rapidly dividing cells in S- or M?phase thereby causing undesired mutagenic and cytotoxic events in proliferative normal cells. (immune-source.com)
- Radiosensitizers are chemical agents that have the potential to increase the lethal effect of radicals induced during irradiation [1-6].The extent of radiation damage to cells is dependent on the amount of oxygen available to the cell [1,2,7,8]. (who.int)
Unclear1
- It remains unclear how important this effect is or if people taking chemotherapy should take taurine supplements. (peacehealth.org)
Proliferative1
- Actives that protected against induced senescence with no cellular proliferative activity. (imedpub.com)
Endothelial cells1
- Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. (thermofisher.com)
Significantly2
- In another study, patients receiving high-dose paclitaxel and melphalan had significantly fewer episodes of oral ulcers and bleeding when they took 6 grams of glutamine four times daily along with the chemotherapy. (peacehealth.org)
- By flow cytometry, many fewer NK function-activating receptors and significantly more killer cell Iglike were found to be expressed on peripheral blood CD56 + NK cells of healthy controls than on those of AA patients. (imedpub.com)
Proteins2
- The protection induced by respond by trying to overproduce ribosomal proteins. (cdc.gov)
- Like nutlin-3 they induce p53 proteins and transcriptional activity however they differ. (immune-source.com)
Cancers3
- The Cdk5-AMT signal pathway involved in regulation of DDR by TIGAR defines a new role of TIGAR in cancer cell survival and it suggests that TIGAR may be a therapeutic target for cancers. (nature.com)
- Though cancer cells use glutamine as a fuel source, studies in humans have not found that glutamine stimulates growth of cancers in people taking chemotherapy. (peacehealth.org)
- Keywords: actinomycin D chemoprotection cyclotherapy leptomycin B Nutlin-3 p53 tenovin-6 Launch Chemotherapy approaches purpose at developing remedies that eradicate cancers cells effectively and selectively. (immune-source.com)
Tissues1
- Cyclotherapy strategies purpose at improving the therapeutic windows of standard chemotherapy by protecting healthy cells.1-3 For instance if transient cell cycle arrest ARRY334543 (Varlitinib) in G1 or G2 was induced in normal tissues only their level of sensitivity to S- and M-phase poisons would be decreased while leaving the tumor vulnerable to the treatment. (immune-source.com)
Therapeutic1
- Recent studies have suggested that extracellular vesicles (EVs) secreted from mesenchymal stem cells (MSCs) exert therapeutic effects in various degenerative diseases. (zoores.ac.cn)
Toxicity1
- It is worth mentioning that many of the electron-affinic radiosensitizers of hypoxic cells also have the property of preferential or selective toxicity directed against hypoxic cells even in the absence of radiation [1,2]. (who.int)
Regeneration2
- We could not predict that prolonged fasting would have such a remarkable effect in promoting stem cell-based regeneration of the hematopoietic system," said corresponding author Valter Longo , the Edna M. Jones Professor of Gerontology and the Biological Sciences at the USC Davis School of Gerontology , and director of the USC Longevity Institute. (usc.edu)
- During each cycle of fasting, this depletion of white blood cells induces changes that trigger stem cell-based regeneration of new immune system cells. (usc.edu)
Processes1
- Brain infection is thought to occur by means of direct neuronal transmission of the virus from a peripheral site to the brain via the trigeminal or olfactory nerve and indirect immune-mediated processes inducing neuroinflammation. (medscape.com)
Pathway1
- Previous study revealed that the protective effect of TIGAR in cell survival is mediated through the increase in PPP (pentose phosphate pathway) flux. (nature.com)
Peripheral blood2
- CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. (thermofisher.com)
- Applications Tested: This 12G5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. (thermofisher.com)
Effectiveness2
- Vitamin C appears to increase the effectiveness of chemotherapy in animals and with human breast cancer cells in test tube research. (peacehealth.org)
- In contrast pre-incubation of p53-mutant cells with tenovin-6 or leptomycin B reduces the effectiveness of vinca alkaloids suggesting that these p53 activators could ARRY334543 (Varlitinib) be effective as chemoprotectants if combined with S- but not M-phase poisons. (immune-source.com)
Sensitivity1
- Light sensitivity: This tandem dye is sensitive photo-induced oxidation. (thermofisher.com)
Receptors1
- SEB is a "bacterial superantigen" because it can form a "bridge" between the MHC II on the antigen-presenting cells and the T-cell receptors on both CD4 and CD8 T cells, thereby bypassing the normal antigen processing and presenting mechanism. (medscape.com)
Effects4
- A comprehensive review of antioxidants and chemotherapy leaves open the question of whether supplemental antioxidants definitely help people with chemotherapy side effects, but it clearly shows that antioxidants need not be avoided for fear that the actions of chemotherapy are interfered with. (peacehealth.org)
- It gives the 'okay' for stem cells to go ahead and begin proliferating and rebuild the entire system," explained Longo, noting the potential of clinical applications that mimic the effects of prolonged fasting to rejuvenate the immune system. (usc.edu)
- The results of this study suggest that fasting may mitigate some of the harmful effects of chemotherapy," said co-author Tanya Dorff , assistant professor of clinical medicine at the USC Norris Comprehensive Cancer Center and Hospital. (usc.edu)
- The in vitro effects of metronidazole on the production of reactive oxygen species by polymorphonuclear (PMN) cells were studied by means of nitroblue tetrazolium and luminol-dependent chemiluminescence. (who.int)
Selectively1
- Metronidazole selectively radiosensitizes hypoxic cells without influencing the radiation response of normal well-oxygenated cells [5,7-9]. (who.int)
Excessive1
- The chemotherapy drug cisplatin may cause excessive loss of magnesium and potassium in the urine. (peacehealth.org)
Stem cells2
- The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
- PKA is the key gene that needs to shut down in order for these stem cells to switch into regenerative mode. (usc.edu)
Human3
- What we started noticing in both our human work and animal work is that the white blood cell count goes down with prolonged fasting. (usc.edu)
- However, because few perifollicular NK cells are found around healthy human anagen HFs, we asked how HFs escape from NK cell attack. (imedpub.com)
- A/Swine/Iowa/30 were protected against death and major a new drug that kills bacteria without disturbing the human weight loss and had undetectable virus in respiratory tis- host. (cdc.gov)
Suppression1
- This study suggests that this happens via an active NK cell suppression. (imedpub.com)
White blood2
- The spleen preparation had a significant stabilizing effect on certain white blood cells. (peacehealth.org)
- Prolonged fasting forces the body to use stores of glucose, fat and ketones, but also breaks down a significant portion of white blood cells. (usc.edu)
Resistant1
- Hypoxic cancer cells are known to be relatively resistant to radiation killing compared with well oxygenated healthy cells [2,3,7-9]. (who.int)
Regulation1
- In particular, prolonged fasting reduced the enzyme PKA, an effect previously discovered by the Longo team to extend longevity in simple organisms and which has been linked in other research to the regulation of stem cell self-renewal and pluripotency - that is, the potential for one cell to develop into many different cell types. (usc.edu)
Agents2
- People taking it also experienced stabilized body weight and a reduction in the fatigue and inertia that usually accompany this combination of chemotherapy agents. (peacehealth.org)
- Antimicrobial Agents and Chemotherapy. (cdc.gov)
Cycle1
- The 1st studies used low doses (LD) of DNA damaging providers to induce p53-dependent cell cycle arrest. (immune-source.com)
Response1
- Several small-molecule activators of the p53 tumor suppressor have been shown to induce a slight cytostatic response in normal cells in tradition. (immune-source.com)
Cancer6
- Results showed that TIGAR was increased and relocated to the nucleus after epirubicin or hypoxia treatment in cancer cells. (nature.com)
- We have previously reported that TIGAR plays a pro-survival role in cancer cells through increase PPP flux 19 . (nature.com)
- Patients with inoperable head and neck cancer were treated with a spleen peptide preparation (Polyerga) in a double-blind trial during chemotherapy with cisplatin and 5-FU. (peacehealth.org)
- Today, it constitutes the standard of care for many cancer patients, along with surgery and chemotherapy. (springer.com)
- Pharmacological activation of wild-type p53 has been found to protect normal cells in culture from cytotoxicity and nuclear aberrations caused by standard cancer therapeutics. (immune-source.com)
- Metronidazole (Flagyl) is a drug used to treat infections and has been studied in the treatment of cancer [10,11] as a radiosensitizer drug, especially for hypoxic cells [9]. (who.int)
Drug1
- With this line it has been demonstrated that pre-incubation with LD doxorubicin (LDDOX) protects normal cells against tubulin poisons.4 5 Similarly a LD of actinomycin?D (LDactD) another clinically approved anticancer drug has been used like a chemoprotectant in combination with the Aurora kinase inhibitor VX680.6 Unfortunately both LDDOX and LDactD also protected p53-deficient malignancy cell lines to a small degree. (immune-source.com)
Research3
- A modified form of vitamin A has been reported to work synergistically with chemotherapy in test tube research. (peacehealth.org)
- Although research remains incomplete, the idea that people taking chemotherapy should avoid antioxidants is not supported by scientific research. (peacehealth.org)
- By outlining how prolonged fasting cycles - periods of no food for two to four days at a time over the course of six months - kill older and damaged immune cells and generate new ones, the research also has implications for chemotherapy tolerance and for those with a wide range of immune system deficiencies, including autoimmunity disorders. (usc.edu)
Expression1
- Normally, Natural Killer (NK) cells attack cells with absent/low MHC class I expression. (imedpub.com)
Immune2
- When you starve, the system tries to save energy, and one of the things it can do to save energy is to recycle a lot of the immune cells that are not needed, especially those that may be damaged," Longo said. (usc.edu)
- Now, if you start with a system heavily damaged by chemotherapy or aging, fasting cycles can generate, literally, a new immune system. (usc.edu)
Show1
- We show that the NK cell inhibitor macrophage migration inhibitory factor is strongly expressed by the HF epithelium, and very few NK cells are observed in and around normal anagen HFs compared to AA with prominent aggregations of NK around. (imedpub.com)
Normal cells1
- With this context p53 status provides a way to distinguish between normal cells retaining wild-type p53 and malignancy cells lacking practical p53. (immune-source.com)
Shown1
- Taurine has been shown to be depleted in people taking chemotherapy. (peacehealth.org)
Test2
- A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. (thermofisher.com)
High1
- Herein we report the development of a high-throughput screening assay, using low, repeat dosing of to induce senescence. (imedpub.com)
Amount1
- The amount of Lactobacillus GG used was 10-20 billion organisms per day during the 24 weeks of chemotherapy. (peacehealth.org)