Proteases caspase 10. Medical search. Frequent questions

initiator caspases (caspases 2, 8, 9 and 10), which are able to hydrolyse and activate a second family of effector caspases (caspases 3, 6 and 7), which themselves are able to hydrolyse further cellular proteins to bring about programmed cell death. (guidetopharmacology.org)
caspases (e.g. caspase-3, -6, and -7) in a cascade-like manner, which cleave key cellular proteins that lead to the morphological changes associated with apoptotic cell death. (enzolifesciences.com)
Caspases mediate their effects through aspartate-specific cleavage of their target proteins, and at present almost 400 caspase substrates are known. (biomedcentral.com)
There are several methods developed to predict caspase cleavage sites from individual proteins, but currently none of them can be used to predict caspase cleavage sites from multiple proteins or entire proteomes, or to use several classifiers in combination. (biomedcentral.com)
A database was constructed with the developed tool, and it was used to identify caspase target proteins from tandem mass spectrometry data from two different proteomic experiments. (biomedcentral.com)
We have developed Pripper, a tool for reading an arbitrary number of proteins in FASTA format, predicting their caspase cleavage sites and outputting the cleaved sequences to a new FASTA format sequence file. (biomedcentral.com)
We show that Pripper is a valuable tool in identifying novel caspase target proteins from modern proteomics experiments. (biomedcentral.com)
Caspases mediate their effects through aspartate-specific cleavage of their target proteins, and several hundreds of caspase target proteins have been identified [ 3 ]. (biomedcentral.com)
Studies of amino acids in the motif have revealed that the motif is only moderately conserved between target proteins of different caspases. (biomedcentral.com)
The prediction of caspase cleavage sites from proteins is not a trivial task due to the heterogeneity of target sequences, and several different tools have been developed to predict caspase cleavage sites from individual proteins. (biomedcentral.com)
A proteins [41] exposed that caspases cut proteins mainly in disordered areas or coils, to a lower degree in -helices and hardly ever in -linens. (biopaqc.com)
The Weblogo alignment [29] of supplementary framework components around cleavage sites (20 proteins) demonstrated that 60% of components are symbolized by coils, 30% by -helices, and around 10% by -bed sheets (Amount 2), relative AUY922 inhibition to previously observations [39,41,44] (Desk S6). (biopaqc.com)
Most Vertebrate Caspase Cleavage Sites are Located within Hydrophilic Surroundings Proteins in aqueous conditions, such as the cytosol, tend to have hydrophobic residues hidden within their structure and hydrophilic amino acids exposed to the surface [45]. (biopaqc.com)
A lot of the vertebrate caspase cleavage sites can be found within a hydrophilic environment, needlessly to say (Amount 3a), and so are most likely exposed at the top of proteins. (biopaqc.com)
Protein and gene expression of apoptotic signaling pathway-related proteins such as caspase-3, cytochrome c, and PARP were determined to provide evidence for the mechanisms of action of Tan IIA in the treatment of psoriasis. (hindawi.com)
It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins. (bionotatki.com)
Description: Caspase-1 is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta, interleukin 18, and interleukin 33 precursor proteins. (bionotatki.com)
It belongs to a family of cysteine proteases known as caspases that always cleave proteins following an aspartic acid residue. (bionotatki.com)
Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9. (crossfirebeilstein.com)
Caspases are essential in cells for apoptosis , one of the main types of programmed cell death in development and most other stages of adult life, and have been termed "executioner" proteins for their roles in the cell. (wikidoc.org)
They are called cysteine proteases , because they use a cysteine residue to cut those proteins, and are called caspases because the cysteine residue cleaves their substrate proteins at specific asparagine residues. (wikidoc.org)
BCL2 proteins are described as 'crucial regulators of apoptosis' (PMID:15868100)and the intrinsic apoptosis pathway is initiated by the release of mitochondrial cytochrome c into the cytoplasm, which results in the activation of caspases (15102863) - help in generating a defintion for intrinsic apoptosis? (geneontology.org)
does all activation of caspases require other proteins also involved in apoptosis - therefore shouldn't this term be a part of apoptosis? (geneontology.org)
Caspases are a family of proteases, or enzymes that degrade proteins, which play an essential role in initiating and carrying out programmed cell death in animals. (sciencedaily.com)
We also identified for the first time 10 proteins previously classified by the Human Proteome Organization as "missing" in the human proteome. (ashpublications.org)
PIs also have a high affinity for the catalytic site of HIV-1 protease, which shares a 60% sequence homology with 2 proteins involved in lipid metabolism, cytoplasmic retinoic acid-binding protein type 1 (CRABP-1), and low-density lipoprotein receptor-related protein (LDLR-RP). (medscape.com)
Cystatin A (Cys A), a cysteine protease inhibitor, is a precursor of proteins involves in keratinocyte keratinization, and is expressed during the late phase of differentiation of these cells. (bvsalud.org)

Interestingly, while GT1b enhanced caspase-3 activity, DEVD, a caspase-3 inhibitor, failed to rescue DA neuronal death. (biomedcentral.com)
To this end, the authors generated a transgenic rat line expressing baculovirus p35, a broad-spectrum caspase inhibitor, in central neurons. (asahq.org)
Apoptosis was partially blocked by the caspase-3 inhibitor Ac-DEVD-CHO. (hindawi.com)
This suggests that it might assist the proper folding of this giant protease and offers a first mechanistic explanation for the genetic evidence that securin is not only an inhibitor but also an activator of separase. (uni-bayreuth.de)
The CP inhibitor E-64 and a caspase inhibitor were used to inhibit apoptosis. (avma.org)
The caspase inhibitor reduced the amount of apoptosis in BUECs. (avma.org)
Activity profiling and crystal structures of inhibitor-bound SARS-CoV-2 papain-like protease: A framework for anti-COVID-19 drug design. (salvesenlab.org)
Protease inhibitor studies revealed metalloproteinases as being primarily responsible for proteolytic processing (as opposed to degradation) during storage. (ashpublications.org)
AA005 induces a unique type of cell death in colorectal adenocarcinoma cells, characterized by lack of caspase-3 activation or apoptotic body formation, sensitivity to poly (ADP-ribose) polymerase inhibitor Olaparib (AZD2281) but not pan-caspase inhibitor Z-VAD.fmk, and dependence on apoptosis-inducing factor (AIF). (biomedcentral.com)

Given the role of caspases in apoptosis, caspase inhibitors may provide neuronal protection after cardiac arrest. (asahq.org)
The initiation of this cascade reaction is regulated by caspase inhibitors. (wikidoc.org)
Cell death is frequently considered to be 'caspase-dependent' when it is suppressed by broad-spectrum caspase inhibitors such as N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-fmk). (geneontology.org)
Design, synthesis, and in vitro evaluation of aza-peptide aldehydes and ketones as novel and selective protease inhibitors. (salvesenlab.org)
Most experts currently believe that HIV type 1 (HIV-1) protease inhibitors (PIs) and nucleoside reverse transcriptase inhibitors (NRTIs), especially stavudine and zidovudine, are implicated. (medscape.com)

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. (neuromics.com)
Thereafter, hydrophilicity of cleavage sites facilitating caspase digestion of the protein would make the respective substrates more important for apoptosis. (biopaqc.com)
The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. (crossfirebeilstein.com)
The exact contribution that the cleavage of many caspase substrates makes to the biochemistry and morphology of apoptosis is unclear. (wikidoc.org)
Evaluation of the effects of phosphorylation of synthetic peptide substrates on their cleavage by caspase-3 and -7. (salvesenlab.org)
System-wide identification of metalloproteinase and other proteinase substrates and their respective cleavage sites suggests novel mechanisms of the effect of proteases on protein activity and platelet function during storage. (ashpublications.org)

This enzyme catalyses the following chemical reaction Strict requirement for Asp at position P1 and has a preferred cleavage sequence of Leu-Gln-Thr-Asp!Gly Caspase-10 is an initiator caspase, as are caspase-2 (EC 3.4.22.55), caspase-8 (EC 3.4.22.61) and caspase-9 (EC 3.4.22.62). (wikipedia.org)
Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. (bionotatki.com)

Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. (neuromics.com)
CASP2 , CASP8 , CASP9 and CASP10 ) cleave inactive pro-forms of effector caspases, thereby activating them. (wikidoc.org)
Effector caspases (e.g. (wikidoc.org)
Initiator caspases possess a longer prodomain than the effector caspases, whose prodomain is very small. (wikidoc.org)
This allows them to autoactivate, so that they can then proceed to activate the effector caspases. (wikidoc.org)

The antibody recognizes an epitope in the large domain subunit of Caspase-3. (thermofisher.com)
As such it will recognize pro Caspase-3 and the large subunit cleavage fragment. (thermofisher.com)
Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. (bionotatki.com)
They are first synthesized as inactive pro-caspases , that consist of a prodomain, a small subunit and a large subunit. (wikidoc.org)

This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. (thermofisher.com)
The inflammasome complex subsequently activates Casp-1, which in turn initiates downstream responses, including the maturation and release of interleukin-1β (IL-1β) and IL-18 ( 4 , 5 , 10 , 11 ). (frontiersin.org)
The NLRP3 inflammasome is a multiprotein complex that activates cysteine protease caspase-1, which leads to the maturation and secretion of pro-inflammatory cytokines, such as interleukin-1β (IL)-1β and IL-18 ( 7 ). (spandidos-publications.com)
the apoptosome (regulated by cytochrome c and the Bcl-2 family ) which activates caspase-9 . (wikidoc.org)

Caspase-10 (EC 3.4.22.63, FLICE2, Mch4, CASP-10, ICE-like apoptotic protease 4, apoptotic protease Mch-4, FAS-associated death domain protein interleukin-1beta-converting enzyme 2) is an enzyme. (wikipedia.org)
Caspases are heterotetrameric, being made up of two pairs of subunits, generated by a single gene product, which is proteolysed to form the mature protein. (guidetopharmacology.org)
Full-length recombinant human caspase-3 protein was used as immunogen. (thermofisher.com)
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. (thermofisher.com)
It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. (thermofisher.com)
Initiator caspases (e.g. caspase-8 and -10) are activated by dimerization of the zymogen on a dedicated adaptor protein. (enzolifesciences.com)
Three different pattern recognition classifiers were developed for predicting caspase cleavage sites from protein sequences. (biomedcentral.com)
Pripper is not restricted to predicting only caspase cut sites, but it gives the possibility to scan protein sequences for any given motif(s) and predict cut sites once a suitable cut site prediction model for any other protease has been developed. (biomedcentral.com)
Recognition of the inflammatory ligand results in the formation of inflammasomes ( 3 , 6 ), which are multi-protein signaling hubs that initiate immune responses by recruiting and activating proteases, such as caspase-1 (Casp-1) ( 4 - 7 ). (frontiersin.org)
Full-length recombinant human Caspase 7 protein. (neuromics.com)
The NLRP3 inflammasome consists of the NLRP3 scaffold, the apoptosis-associated speck-like protein containing a caspase recruitment domain (CARD) (ASC) adaptor and caspase-1 ( 5 , 6 ). (spandidos-publications.com)
Modulation of the NALP3 protein inflammasomes induces regulatory T cells to express cytotoxic T-lymphocyte antigen 4, IL-10, and transforming growth factor-beta (TGF-β). (medscape.com)
Horvitz and his colleague Junying Yuan [2] found in 1993 that the protein encoded by the ced-3 gene was a cysteine protease with similar properties to the mammalian interleukin-1-beta converting enzyme (ICE) (now known as caspase 1) which at the time was the only known caspase. (wikidoc.org)
Cytochrome c then forms a multi-protein complex known as the 'apoptosome' and initiates activation of the caspase cascade through caspase 9. (geneontology.org)
Extended subsite profiling of the pyroptosis effector protein gasdermin D reveals a region recognized by inflammatory caspase-11. (salvesenlab.org)
The findings, outlined in a paper titled "VPEg Exhibits a Caspase-like Activity that Contributes to Defense Against Pathogens" were reported in the Sept. 23, online issue of Current Biology, and involve research on the key plant protein, vacuolar processing enzyme or VPEg, in Arabidopsis thaliana, or thale cress, that is required for this process. (sciencedaily.com)
Conclusion: Human AAT is an acute phase protein with a broad-protease inhibitory and immunomodulatory activities used as a therapeutic for emphysema patients with inherited AAT deficiency. (lu.se)

Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. (thermofisher.com)
Caspase-1 is a proteolytic enzyme that processes the inactive precursor of IL-1β into the mature form, and is termed IL-1β converting enzyme (ICE). (spandidos-publications.com)
Moreover, a spectrofluorometric enzyme assay was used to measure the protease activity of caspase-3 and -9. (cdc.gov)
Caspase-9 enzyme activity was similar in the SSC and control samples (P (cdc.gov)
However, the function of ICAD/DFF45 is to restrain the enzyme CAD (Caspase Activated DNase). (wikidoc.org)
Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. (bvsalud.org)

Caspases are a family of proteases that have central functions in programmed cell death (apoptosis) and inflammation. (biomedcentral.com)

NETosis Occurs Independently of Neutrophil Serine Proteases. (salvesenlab.org)
In particular, one collagen and two serine proteases were found to have experienced strong positive selection. (prelekara.sk)
Wang S, Olt S, Schoefmann N, Stuetz A, Winiski A, Wolff-Winiski B. SPINK5 knockdown in organotypic human skin culture as a model system for Netherton syndrome: effect of genetic inhibition of serine proteases kallikrein 5 and kallikrein 7. (medscape.com)

Altogether 15 different caspases have been identified in mammals, and they are grouped into two major subfamilies, inflammatory and apoptotic caspases [ 2 ]. (biomedcentral.com)
reported that NLRP1A generates a functional caspase-1-containing inflammasome in vivo to drive IL-1β-dependent inflammatory disease ( 12 ). (frontiersin.org)
Inflammasomes are intracellular multiprotein complexes that regulate the activity of caspase-1 and can be activated by various cellular dangers that trigger the processing and release of pro-inflammatory cytokines to engage innate immunity ( 3 ). (spandidos-publications.com)
Thus, more macrophages are attracted, causing further release of cytotoxic oxidants and proteases, inflammatory cytokines, and arachidonic metabolites. (medscape.com)
Description: Caspase-3 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. (crossfirebeilstein.com)
Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. (crossfirebeilstein.com)
This is because they are inflammatory caspases, which in concert with CASP1 , are involved in cytokine maturation. (wikidoc.org)
Resurrection of an ancient inflammatory locus reveals switch to caspase-1 specificity on a caspase-4 scaffold. (salvesenlab.org)

Activation of caspases-8 and -10 by FLIP(L)". The Biochemical Journal. (wikipedia.org)
Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. (thermofisher.com)
Processing of caspases is required for activation of downstream signaling and dsRNA stimulation inducese the processing of these caspases. (reactome.org)
The nonapoptotic caspase function of both caspase-8 and -10 does not require the protease activity and the DED-containing prodomains are sufficient for NF-kB activation. (reactome.org)
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. (sigmaaldrich.com)
Involved in the activation cascade of caspases responsible for apoptosis execution. (lu.se)
We demonstrated that UVB irradiation induces IL-1β upregulation and IL-1β-dependent inflammation via caspase-1 activation in these Nlrp1b KI mice. (frontiersin.org)
the second stimulus is DAMPs that induce the activation of caspase-1 ( 3 , 5 , 8 , 9 ). (spandidos-publications.com)
and second, the activation of the cysteine protease, caspase-1 ( 15 ). (spandidos-publications.com)
These findings suggest that neuronal apoptosis after cardiac arrest is not primarily initiated by activation of caspases. (asahq.org)
Mitochondrial regulation of apoptosis further downstream was investigated, showing changes in the mitochondrial membrane potential, cytochrome c release into the cytoplasm, and enhanced activation of cleaved caspase-3 and Poly ADP-ribose polymerase (PARP). (hindawi.com)
A fluorometric assay was used to detect BUEC caspase 3 activation. (avma.org)
Apoptotic signaling is initiated either through extrinsic or intrinsic stimulation, resulting in the activation of caspases [ 12 ]. (biomedcentral.com)
The prodomain of the initiator caspases contain domains such as a CARD domain (e.g. caspases-2 and -9) or a death effector domain (DED) (caspases-8 and -10) that enables the caspases to interact with other molecules that regulate their activation. (wikidoc.org)
should the term ['GO:0006919] activation of caspase activity' be a child of positive regulation of apoptosis or induction of apoptosis rather than directly to apoptosis? (geneontology.org)
Endothelial activation of caspase-9 promotes neurovascular injury in retinal vein occlusion. (salvesenlab.org)
Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur. (bvsalud.org)

Our data provide novel evidence that a Akt/GSK-3β/tau-dependent, but not caspase-3 signaling pathway plays a pivotal role in GT1b-mediated neurotoxic actions on mesencephalic DA neurons. (biomedcentral.com)
There was also no translocation of apoptosis-inducing factor (AIF) from mitochondria to the nucleus in apoptotic keratinocytes, indicating Tan IIA-induced apoptosis occurs mainly through the caspase pathway. (hindawi.com)
After polymers produced by 2-5OAS activate RNase L, RNase L begins to fragment mRNA and upregulates an apoptotic pathway involving caspases. (phoenixrising.me)
10) Cleavage of Mcl1 and Bcl-xL by separase transforms these pro-survival factors into proapoptotic fragments and triggers death in mitosis via the intrinsic pathway of apoptosis. (uni-bayreuth.de)
AA005 may trigger the cell death via mediated by AIF through caspase-3 independent pathway. (biomedcentral.com)

However, the level of apoptotic DNA fragmentation, cytochrome c, and caspases-3 protease activity tended to increase by 11% (P=0.056), 30% (P=0.061), and 39% (P=0.088) in the SSC muscle when compared to contralateral control muscle, respectively. (cdc.gov)
In response to apoptotic stimulus, permeabilized mitochondria release cytochrome c into the cytoplasm, where cytochrome c forms an apoptosome with Apaf-1 and caspase-9 and triggers the caspase cascade. (biomedcentral.com)

Curiously, the prevalence value for the two thirds of the human being caspase targets is around 1, suggesting that there is mostly no difference between the percentage of coils in areas immediately surrounding the cleavage site AUY922 inhibition and in more distant sequences (Number 2b). (biopaqc.com)

The following product was used in this experiment: Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) from Thermo Fisher Scientific, catalog # MA1-91637, RRID AB_1954999. (thermofisher.com)
Western Blot analysis of (A) RAW264.7 and (B) NIH-3T3 cell lysate using Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) (Product # MA1-91637). (thermofisher.com)
Human IgG antibody Laboratories manufactures the signalway antibody caspase-1 reagents distributed by Genprice. (bionotatki.com)
The Signalway Antibody Caspase-1 reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (bionotatki.com)
Caspase Assay Laboratories manufactures the caspase 3 antibody santa cruz reagents distributed by Genprice. (crossfirebeilstein.com)

caspases are synthesized as inactive zymogens that can be cleaved to form active enzymes following the induction of apoptosis by stress or death receptors. (enzolifesciences.com)
Until then, this essential protease is kept inactive by association with securin. (uni-bayreuth.de)
Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. (crossfirebeilstein.com)

Description: Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. (bionotatki.com)
Description: CASP3 is a member of the cysteine-aspartic acid protease (caspase) family. (crossfirebeilstein.com)

Evaluation of the classifiers with quality measures indicated that all of the three classifiers performed well in predicting caspase cleavage sites, and when combining different classifiers the accuracy increased further. (biomedcentral.com)
b) Distribution of coil prevalence beliefs of individual caspase cleavage sites, computed as defined in the written text. (biopaqc.com)

The Caspase-8 substrate and pNA standard are especially light sensitive. (sigmaaldrich.com)
We further created the thought of caspase structural choices and hypothesized the substrate cleavage site should be more accessible for proteolysis if it is located not only in an unstructured region, but within the loop between two organized areas. (biopaqc.com)

121104-96-9 For caspase inhibition, cells have been preincubated for one h with 10 mM of the pancaspase inhibitior zVAD-fmk (R&D Systems, Wiesbaden, Germany), which binds the lively web sites of caspase-like proteases. (gpr44.com)
[ 8 ] Caspase inhibition in clonal striatal cells showed no correlation between the reduction of aggregates in the cells and increased survival. (medscape.com)

Both known caspase cleavage products as well as novel cleavage products were identified using the database demonstrating the usefulness of the tool. (biomedcentral.com)

initiator (apical) caspases and effector (executioner) caspases . (wikidoc.org)

Caspases have the ability to cleave after aspartic acid residues. (neuromics.com)

In addition, using immunohistochemical staining and quantification of terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL)-positive and activated caspase-3-positive cell death after intraperitoneal LPS challenges, we addressed the hypothesis that this exaggerated inflammation exacerbates the neurodegenerative process. (jneurosci.org)
Caspases are a family of calcium -dependent cysteine proteases , which play essential roles in apoptosis (programmed cell death), necrosis and inflammation . (wikidoc.org)
Caspase mechanisms in the regulation of inflammation. (salvesenlab.org)

The aim of this study was to evaluate serum levels of caspase-3, caspase-8, and caspase-9 in women with ovarian cancer. (mdpi.com)
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. (epigentek.com)
HCC cell lines were maintained in high glucose DMEM (Invitrogen, California, USA) supplemented with 10% fetal bovine serum (FBS) (Life Technologies) and 1% (100 U/mL) penicillin-streptomycin (Life Technologies), then sustained at 37°C in a humidified incubator at 5% CO 2 . (hindawi.com)

The caspases are a family of cysteine proteases that cleave after certain aspartate residues, and are primarily recognized as mediators of apoptosis. (enzolifesciences.com)

This caspase-cascade plays a central role in the induction and transduction of apoptotic signals, as well as in the regulation of immunity, cellular proliferation and differentiation [ 2 ]. (biomedcentral.com)
Our results suggest that the NLRP3/ASC/caspase-1 axis participates in the regulation of pro-imflammatory cytokine secretion in RAW264.7 cells, particularly the generation of IL-1β and IL-18. (spandidos-publications.com)
Regulation of the immune response involves the suppression of type 2 THCs (involved in humoral immunity) by interferon gamma and the production of IL-10 by type 2 THCs, which inhibits type 1 THCs. (medscape.com)

The most important caspase in this cascade is caspase-3, which is cleaved and activated to transduce the apoptotic signal [ 6 , 7 ]. (biomedcentral.com)

The caspases can be activated through either the intrinsic (mitochondrial mediated) or extrinsic (death receptor mediated) apoptotic pathways. (geneontology.org)

Following this discovery, the other mammalian caspases, in addition to caspases in other organisms such as the fruit fly Drosophila melanogaster , were soon identified and characterised. (wikidoc.org)

The cone-enriched apoptotic protease caspase-7 ( Casp7 ) is thought to be triggered by endoplasmic reticulum (ER) stress and plays a pivotal role in mice deficient in the cone cyclic nucleotide-gated channels, a deficiency that causes achromatopsia in humans and in mice with autosomal dominant rhodopsin mutations, in particular the T17M mutation. (molvis.org)

Caspase 8 Colorimetric Activity Assay Kit, IETD Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD. (sigmaaldrich.com)

Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-8 activity. (sigmaaldrich.com)
Induction of apoptosis by CP30 is correlated with protease activity. (avma.org)
Although the presence of active caspases and DNA fragmentation is helpful in identifying possible apoptosis, they should not be employed as an exclusive means to demonstrate this process as apototic cell death can occur without DNA fragmentation or caspase activity. (geneontology.org)
Protease activity of the API2-MALT1 fusion oncoprotein in MALT lymphoma development and treatment. (medscape.com)

The Caspase-1 gene consists of 10 exons spanning at least 10.6 kb and is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas. (bionotatki.com)
The Caspase-1 gene consists of 10 exons spanning at least 10.6 kb and is mapped to 11q23, a site frequently involved in rearrangement in human cancers, including a number of leukemias and lymphomas, by Southern DNA blot analysis of rodent-human hybrids and by in situ hybridization to normal human metaphase chromosomes. (bionotatki.com)
The importance of caspases to apoptosis and programmed cell death was originally established by Robert Horvitz and colleagues who found that the ced-3 gene was required for the cell death that took place during the development of the nematode C. elegans . (wikidoc.org)
Genetic errors of the human caspase recruitment domain-B-cell lymphoma 10-mucosa-associated lymphoid tissue lymphoma-translocation gene 1 (CBM) complex: Molecular, immunologic, and clinical heterogeneity. (medscape.com)

Zika virus NS3 protease induces fetal brain calcification. (ccf.org)
Objective -To determine the effects of 2 conjugated linoleic acid (CLA) isomers (cis-9, trans-11 and trans-10, cis-12) on synthesis of prostaglandin (PG) E 2 and F 2α and expression of prostaglandin H synthase-2 (PGHS-2) of adult and fetal bovine endometrial epithelial cells in vitro. (avma.org)

Thus, identifying a specific caspase as part of a cell death mechanism can give insights into the underlying cause for cell death. (molvis.org)
Caspase 1 has been shown to induce cell necrosis or pyroptosis and may function in various developmental stages. (bionotatki.com)
It was purified from the cytosol of the THP monocytic cell line and found that the active protease was made up of two peptides, which they called p20 and p10 based on their apparent molecular masses by SDS-PAGE. (bionotatki.com)
Cell lines have been cultured at 37uC, five% CO2 in DMEM (Gibco, Karlsruhe, Germany) supplemented with ten% FCS and antibiotics (Biochrom, Berlin, Germany). (gpr44.com)
Apoptosis is a programmed form of cell death involving the degradation of cellular constituents by a group of cysteine proteases called caspases. (geneontology.org)
Caspase-like activities have also been shown to be required for the initiation of programmed cell death in plants, but the genes controlling those activities have not been identified. (sciencedaily.com)
Here we report that AA005 may trigger caspase-3-independent cell death, mediated by AIF. (biomedcentral.com)

Our data further confirm that caspase-3 is not a major effector of DA neuronal death in GT1b neurotoxicity. (biomedcentral.com)

Anatomy21

Organisms8

Diseases3

Chemicals and Drugs151

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes52

Disciplines and Occupations1

Information Science4

Health Care1

Proteins27

Inhibitor9

Inhibitors5

Substrates6

Initiator caspase2

Effector caspases5

Subunit4

Activates4

Protein17

Enzyme6

Family of proteases1

Serine Proteases3

Inflammatory8

Activation17

Pathway5

Cytochrome2

Human being c1

Antibody5

Inactive3

Cysteine-aspartic2

Cleavage sites2

Substrate2

Inhibition2

Known caspase1

Executioner caspases1

Aspartic acid1

Inflammation3

Serum3

Residues1

Regulation3

Cascade1

Mitochondrial1

Mammalian1

Casp71

Assay Kit1

Activity4

Gene4

Fetal2

Cell7

Neuronal1