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  • Manipulating the Mouse Embryo
  • This fourth edition of The Mouse Manual - Manipulating the Mouse Embryo - appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. (cshlpress.com)
  • The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. (cshlpress.com)
  • ICSI
  • The high prevalence of oocytes with CLCG over 75% has a negative effect on embryos and the general ICSI clinical outcomes. (biomedcentral.com)
  • Urman B , Ata B, Yakin K, Alatas C, Aksoy S, Mercan R, Balaban B. Luteal phase empirical low molecular weight heparin administration in patients with failed ICSI embryo transfer cycles: a randomized open-labeled pilot trial. (bulenturman.com)
  • bovine
  • When comparing immunohistochemical patterns of antibodies against 5-methyl-cytosine, H3K4me3 and H3K9me2 modifications we observe similar pronuclear distribution and dynamics in mouse, bovine and rabbit zygotes. (biomedcentral.com)
  • transfer
  • Clinical pregnancy rate per embryo transfer was lower in the antagonist group than in the agonist group (30.8% vs. 40.4%) although this difference did not reach statistical significance (P = 0.17). (biomedcentral.com)
  • 0.05) of gestational sacs vanished in the group with exclusive transfer of pitted embryos compared to the unaffected group. (springer.com)
  • An evaluation of the effect of anesthetic technique on reproductive success after laparoscopic pronuclear stage transfer. (springer.com)
  • It also serves as a fundamental technique in the preparation of recipients and donors for embryo transfer procedures, as well as in the recovery of a large number of embryos used later for both biotechnological purposes and in basic studies. (biomedcentral.com)
  • Levels of mtDNA carryover ranged from 0-5% with the pronuclear method and 0-2% in the spindle transfer method. (mitochondrialdiseasenews.com)
  • show that pronuclear transfer could eliminate defective mitochondria . (edu.au)
  • The objective of this work was to determine which embryonic morphokinetic parameters up to D3 of in vitro development have predictive value for implantation for the selection of embryos for transfer in clinical practice based upon information generated from embryo transfers with known implantation data (KID). (springer.com)
  • A total of 800 KID embryos (100% implantation rate (IR) per transfer and 0% IR per transfer) cultured in an incubator with Time-Lapse system were retrospectively analysed. (springer.com)
  • IVF with Blastocyst Transfer Centres in Dehradun are pre-eminent and globally benchmarked fertility and IVF Centres with world-class infrastructure and cutting-edge technology. (elawoman.com)
  • When the embryo is ready for transfer it is called early blastocyst. (elawoman.com)
  • This process is referred to as embryo transfer. (elawoman.com)
  • The embryos can develop in an incubator up to the 5thday, called the blastocyst stage due to improved cell culture media (blastocyst culture) at IVF with Blastocyst Transfer Centres in Dehradun. (elawoman.com)
  • Although fresh embryo transfer is the golden standard in IVF and millions of couples worldwide achieved a live birth, huge progress has been recorded in various aspects of Assisted Reproduction. (frontiersin.org)
  • parthenogenetic
  • Konyukhov, B. 2005-04-27 00:00:00 We studied the effects of three growth factors, fibroblast growth factor (FGF4), transforming growth factor α (TGFα), and transforming growth factor β1 (TGFβ1), on development of diploid parthenogenetic embryos of C57BL/6 mice, which are not capable of developing to somatic stages. (deepdyve.com)
  • When FGF4 or TGFβ1 2.4 were added to the nutrient medium, 2.4 or 1.6%, respectively, of parthenogenetic embryos reached the somatic stages in utero. (deepdyve.com)
  • In the latter case, some parthenogenetic embryos reached the stage of 25-27 pairs of somites and were 2.0-2.5 mm long. (deepdyve.com)
  • The data we obtained suggest that, when combined, the growth factors FGF4, TGFα, and TGFβ1 possessed a synergistic effect leading to a significant improvement of the development of parthenogenetic C57BL/6 embryos. (deepdyve.com)
  • Pubmed ID: 11779183 Paternally expressed imprinted genes (Pegs) were systematically screened by comparing gene expression profiles of parthenogenetic and normal fertilized embryos using an oligonucleotide array. (jove.com)
  • Platonov, E. 2010-04-11 00:00:00 The action of two types of substances has been studied: 5-azadeoxycytidine and retinoic acid, which have a demethylation effect on DNA in the development process of diploid parthenogenetic mouse embryos. (deepdyve.com)
  • The effect of 5-azadeoxycytidine on hybrid mice (CBA × C57BL/6)F1 in vitro for 6 h, in the presence of single cell parthenogenetic embryos during the S-phase of the cell cycle has been studied. (deepdyve.com)
  • post-implantation
  • Electrophysiological systems utilizing negative capacitance have been routinely used for microinjection of a variety of biomolecules into mammalian oocytes, as well as pre-implantation and post-implantation embryos in develomental biology studies.1-6 The system which is no longer available, the intracellular amplifier WPI Cyto721, allows the needle to pierce the cell membrane with minimal physical trauma. (wpiinc.com)
  • cytoplasmic
  • Rienzi L, Ubaldi F, Minasi MG, Iacobelli M, Martinez F, Tesarik J, Greco E: Blastomere cytoplasmic granularity is unrelated to developmental potential of day 3 human embryos. (springer.com)
  • Our cytoplasmic injection protocol improved generation of transgenic embryos and resulted in the first transgenic buffalo calves produced by this method. (biomedcentral.com)
  • viability
  • More recently this technique has been applied to the microinjection of CRISPR/Cas9 reagents into two-cell stage mouse embryos.7 The authors demonstrated significant increase in the knock-in efficiency and high viability of embryos using their method. (wpiinc.com)
  • In contrast to conventional microinjection, in targeted microinjection using the MICRO-ePORE™, the membrane does not tear and thus allows for superior viability of embryos. (wpiinc.com)
  • MICRO-ePORE™ was designed for a range of applications including generation of CRISPR/Cas9 mediated knock-in mice with large insertions by microinjection into two-cell stage embryos with high viability.7 The MICRO-ePORE™ has delivered accurate microinjection of morpholino oligomers (anti-sense "knockdown") in zebrafish tails. (wpiinc.com)
  • early
  • The rate of mature oocytes, rate of presence of multinucleated blastomers, amount of fragmentation in embryos and rate of early-cleaved embryos was similar in the two groups. (biomedcentral.com)
  • Past attempts to produce human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryos. (pubmedcentralcanada.ca)
  • The roadblock responsible for failure is early embryonic arrest of human SCNT embryos precluding derivation of stable NT-ESCs. (pubmedcentralcanada.ca)
  • Notably, the mouse oocyte and early embryo are transcriptionally inactive. (mdpi.com)
  • Full understanding of post-transcriptional regulation of gene expression in early embryos is crucial to understand the reprogramming of the embryonic genome, it might help driving reprogramming of stem cells in vitro and will likely improve in vitro culturing of mammalian embryos for assisted reproduction. (mdpi.com)
  • We will review here the current knowledge on the post-transcriptional control of gene expression in mouse early embryos and discuss some of the unanswered questions concerning this fascinating field of biology. (mdpi.com)
  • somatic
  • Gene expression and differentiation profiles in human NT-ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming of somatic cells to a pluripotent state. (pubmedcentralcanada.ca)
  • time-lapse
  • Hashimoto S, Kato N, Saeki K, Morimoto Y. Selection of high-potential embryos by culture in poly (dimethylsiloxane) microwells and time-lapse imaging. (springer.com)
  • Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis. (springer.com)
  • embryonic genome
  • This oocyte-to-embryo transition needs an efficient and tightly regulated translation of the maternally-inherited mRNAs, which likely contributes to embryonic genome activation. (mdpi.com)
  • mitochondria
  • Another concern is that the group focusing on PNT in the UK has shown that some of the mutated mitochondria are transferred to the new embryo (6). (ivf.net)
  • Elective
  • Elective freezing of all embryos, followed by frozen-thawed ET cycles emerged to prevent risk of Ovarian Hyperstimulation Syndrome and to allow endometrium recovery after Controlled Ovarian Stimulation, leading to better IVF outcomes. (frontiersin.org)