Promoting the phosphorylation. Medical search. Frequent questions

Seahorse was used to measure the level of oxidative phosphorylation. (biomedcentral.com)
In the early reprogramming process, fatty acid oxidation upregulated oxidative phosphorylation and downregulated protein kinase C activity. (biomedcentral.com)
We demonstrated that fatty acid oxidation promotes reprogramming by enhancing oxidative phosphorylation and inhibiting protein kinase C activity in the early stage of the reprogramming process. (biomedcentral.com)
Severe overdose causes impaired oxidative phosphorylation and mitochondrial dysfunction, which can result in cellular death. (medscape.com)
Inhibition of oxidative phosphorylation will promote anaerobic metabolism. (medscape.com)
Glucose is the major substrate for ATP synthesis through glycolysis and oxidative phosphorylation (OXPHOS), whereas intermediary metabolism through the tricarboxylic acid (TCA) cycle utilizes non-glucose-derived monocarboxylates, amino acids, and alpha ketoacids to support mitochondrial ATP and GTP synthesis. (molvis.org)
Mitochondria are the powerhouses that generate energy by oxidative phosphorylation (OXPHOS) to support cellular activities. (upstate.edu)

Tyrosine phosphorylation sites of PIK3CA/p110α are identified with Mass-Spectrum. (biomedcentral.com)
Phospho-proteomics analysis and Western blots are used to demonstrate downstream signaling pathways of PIK3CA/p110α tyrosine phosphorylation. (biomedcentral.com)
In vitro kinase assay is applied to identify the kinase of PIK3CA/p110α tyrosine phosphorylation. (biomedcentral.com)
Tyrosine phosphorylation of PIK3CA/p110α is stimulated by growth factors such as EGF, HGF and PDGF. (biomedcentral.com)
Tyrosine phosphorylation of RTKs, or adaptor proteins such as IRS-1 facilitate their binding with p85 regulatory subunits of PI3K, and then bring PI3K complex on membrane [ 2 ]. (biomedcentral.com)
β1 integrin tyrosine phosphorylation by oncogenic kinases, such as Src, has been predicted to induce tumorigenesis by disrupting adhesion and modifying integrin signaling. (iucc.ac.il)
We conclude that a Src/ FAK signaling unit inhibits differentiation to promote tumorigenesis downstream of β1 integrin and independent of βintegrin tyrosine phosphorylation. (iucc.ac.il)
Investigation of the relationship between PTN and VEGF revealed that PTN, through the PTN/RPTPβ/ζ signaling pathway, increased tyrosine phosphorylation of β-catenin, leading to an increase in VEGF. (nih.gov)
Indeed, we found that rapsyn associated with CD4-β loop chimeras in a phosphorylation-dependent manner, and that agrin increased the ratio of rapsyn binding to wild type AChR but not to AChR-β 3F/3F , which lacks β loop tyrosine phosphorylation sites. (jneurosci.org)
Indeed, we have shown previously that mutation of a tyrosine phosphorylation site in the long cytoplasmic loop of the β subunit impairs agrin-induced cytoskeletal anchoring and aggregation of mutant AChR in muscle cells ( Borges and Ferns, 2001 ). (jneurosci.org)

Interestingly, inhibition of FAK signaling pathway attenuated the phosphorylation of AKT, but inhibition of AKT signaling pathway did not affect the phosphorylation of FAK. (oncotarget.com)
Post-translational modifications (PTMs) are crucial for regulating PI3K/AKT signaling pathway, notably phosphorylation. (biomedcentral.com)
Required for phosphorylation of GLI transcription factors which inhibits them and prevents transcriptional activation of Hedgehog signaling pathway target genes (By similarity). (rcsb.org)

We studied here phosphorylation of 5-HT 1A R by cyclin-dependent kinase 5 (Cdk5), a neuron-specific membrane-bound Ser/Thr kinase that is activated by binding of the p35 Cdk5 activator. (elsevierpure.com)
Cyclin-dependent kinase 5 promotes proteasomal degradation of the 5-HT 1A receptor via phosphorylation Biochemical and Biophysical Research Communications , 510 (3), 370-375. (elsevierpure.com)
NO deficiency appears to lower neuronal calpain nitrosylation, resulting in enzyme activation, which, in turn, leads to tau phosphorylation by activating cyclin-dependent kinase. (genewhisperer.com)

Inhibition of protein kinase C also promoted reprogramming. (biomedcentral.com)

The phosphorylation stimulated the degradation of 5-HT 1A R by the proteasome, resulting in neutralization of the inhibitory action of 5-HT 1A R on intracellular cAMP concentration. (elsevierpure.com)
These results suggest that Cdk5-p35 modulates 5-HT signaling through phosphorylation-dependent degradation of 5-HT lA R. (elsevierpure.com)
NSP1 promotes cellular mRNA degradation and prevents host mRNA translation. (cdc.gov)
Phytochromes enhance SOS2-mediated PIF1 and PIF3 phosphorylation and degradation to promote Arabidopsis salt tolerance. (bvsalud.org)

Here we showed that social defeat stress enhanced plantar incision-induced AMPA receptor GluA1 phosphorylation at the Ser831 site in the spinal cord and greatly prolonged plantar incision-induced pain. (jneurosci.org)
In addition, stress hormones enhanced GluA1 phosphorylation and AMPA receptor-mediated electrical activity in the spinal cord. (jneurosci.org)
Therefore, spinal AMPA receptor phosphorylation contributes to the mechanisms underlying stress-induced pain transition. (jneurosci.org)
We showed that the pheromone-responsive Gβγ dimer promotes the polarization of the pheromone receptor by interacting with Yck1/2 and locally inhibiting receptor phosphorylation. (johnshopkins.edu)
We also found that receptor phosphorylation is essential for chemotropism, independently of its role in inducing receptor internalization. (johnshopkins.edu)
A mathematical model supports the idea that the interaction between Gβγ and Yck1/2 results in differential phosphorylation and internalization of the pheromone receptor and accounts for its polarization before the initiation of directed secretion. (johnshopkins.edu)
Activated nongenomically by L-thyroxine (T4), mitogen-activated protein kinase (MAPK) complexed in 19-20 min with endogenous nuclear thyroid hormone receptor (TRβ1 or TR) in nuclear fractions of 293T cells, resulting in serine phosphorylation of TR. (tmu.edu.tw)
Transfection into CV-1 cells of TR gene constructs in which one or both zinc fingers in the TR DNA-binding domain were replaced with those from the glucocorticoid receptor localized the site of TR phosphorylation by T4-activated MAPK to a serine in the second zinc finger of the TR DNA-binding domain. (tmu.edu.tw)
Here, we define a phosphorylation-dependent binding site on the receptor that mediates agrin-induced clustering. (jneurosci.org)
Together, these findings suggest that agrin-induced phosphorylation of the β subunit motif increases the stoichiometry of rapsyn binding to the AChR, thereby helping to stably cluster the receptor and anchor it at high density in the postsynaptic membrane. (jneurosci.org)
To analyze whether and how the quantitative ratio between receptor and rafts affects LRP6 phosphorylation and WNT/β-catenin pathway activation, we present a computational modeling study, that for the first time employs realistic raft numbers in a compartment-based pathway model. (frontiersin.org)
Our simulation experiments indicate, that for receptor/raft ratios smaller than 1, i.e., when the number of raft compartments clearly exceeds the number of pathway specific membrane proteins, we observe significant decrease in LRP6 phosphorylation and downstream pathway activity. (frontiersin.org)
Here, we apply computational modeling to analyze whether and how the quantitative ratio between receptor and rafts affects LRP6 phosphorylation and WNT/β-catenin pathway activation. (frontiersin.org)
Human cytomegalovirus G protein-coupled receptor US28 promotes latency by attenuating c-fos. (uc.edu)
Platelet receptors for ADP include the P2Y12 receptor, which sends signals to suppress adenylate cyclase, decreases cyclic adenosine monophosphate (cAMP) levels, and promotes activation of the glycoprotein IIb/IIIa receptor (assembled on the activated platelet surface membrane from glycoproteins IIb and IIIa). (msdmanuals.com)

Treatment of cells with the MAPK kinase inhibitor, PD 98059, prevented both T4-induced nuclear MAPK-TR co-immuno-precipitation and serine phosphorylation of TR. (tmu.edu.tw)
Thus, T4 activates MAPK and causes MAPK-mediated serine phosphorylation of TRβ1 and dissociation of TR and the co-repressor SMRT. (tmu.edu.tw)

Protein phosphorylation is crucial for signaling transduction of this pathway. (biomedcentral.com)
Her expertise in protein phosphorylation and biological mass spectrometry have resulted in a number of professional appointments[citation needed]. (wikipedia.org)
Her most cited publications[citation needed] focus on biochemical, structural, cellular and gas-phase studies of protein phosphorylation, protein kinase complexes and glycan conformation, and several highly-accessed reviews[citation needed] on the subject of ion mobility mass spectrometry for biological molecules. (wikipedia.org)
She is highly cited[citation needed] for her analysis of protein phosphorylation by mass spectrometry, and in 2019 work in her lab led to the discovery that non-canonical protein phosphorylation on a broad range of amino acids, including Arg, Asp, Cys, Glu, His and Lys is widespread in human cells. (wikipedia.org)

M2.2 protein interferes with MAVS and inhibits IRF7 phosphorylation. (cdc.gov)

PIP3 phosphatase PTEN is inactivated upon phosphorylation in its C-terminal region by several serine/threonine kinases [ 12 ]. (biomedcentral.com)
We herein demonstrated that CADM1 interacts with Hippo pathway core kinases and enhances the phosphorylation of YAP1, and also that the membranous co-expression of CADM1 and LATS2 predicts a favorable prognosis in lung adenocarcinoma. (cancerindex.org)

cSH2 domain S690 phosphorylation decreases p85 binding affinity to tyrosine-phosphorylated proteins resulting in less PI3K membrane localization [ 15 ]. (biomedcentral.com)

Phosphorylation of Clb5-specific targets during S phase was reduced by replacing Clb5 with Clb2 or by mutating the substrate RXL motif, confirming the importance of Clb5 specificity in vivo . (nature.com)
Figure 3: Clb5-specific substrate phosphorylation occurs in vivo . (nature.com)

Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. (rcsb.org)
Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. (rcsb.org)
The phosphorylation of MLC primarily regulates the force production and maintenance in the smooth muscle [ 6 ]. (hindawi.com)

The regulation of HIF-1α occurs at the posttranslational level involving modifications of hydroxylation, acetylation and phosphorylation ( 3 - 7 ). (spandidos-publications.com)

EA caused a significant increase in the MLC dephosphorylation and MLCK phosphorylation levels in the group compared to the sham EA and model groups. (hindawi.com)
It is suggested that the phosphorylation of MLC catalyzed by Ca 2+ -calmodulin- (CAM-) dependent MLCK and dephosphorylation catalyzed by MLCP help regulate the contraction and relaxation of smooth muscle [ 8 , 9 ]. (hindawi.com)

First, authors investigated whether an HSD induces phosphorylation of tau. (genewhisperer.com)

Although we did not identify any highly Clb2-specific substrates, we found that Clb2-Cdk1 possessed higher intrinsic kinase activity than Clb5-Cdk1, enabling efficient phosphorylation of a broad range of mitotic Cdk1 targets. (nature.com)

Overexpression of AtVPS34 promoted stomatal closure while PI3K inhibitors blocked that after spray inoculation. (frontiersin.org)

PDE4D3 levels in the RyR2 complex were reduced in failing human hearts, contributing to PKA-hyperphosphorylated, "leaky" RyR2 channels that promote cardiac dysfunction and arrhythmias. (nih.gov)

Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation (By similarity). (rcsb.org)

Collectively, these results unraveled a novel and broad role of PI3K in plant immunity which promoted stomatal closure and PR gene expression possibly via regulating ROS production. (frontiersin.org)
For instance, it can be activated by oncogenic mutations of PTEN, VHL, the RAS/mitogen-activated protein kinase (MAPK) pathway and the phosphorylation of phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway. (spandidos-publications.com)

Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR (By similarity). (rcsb.org)

RIOK2 phosphorylation by RSK stimulates cytoplasmic maturation of late pre-40S particles, which is required for optimal protein synthesis and cell proliferation. (iric.ca)
Phosphorylation of pheromone receptors by yeast casein kinase 1 or 2 (Yck1/2) stimulates their internalization. (johnshopkins.edu)
PSMC5/RPT6 activation by phosphorylation stimulates proteasome. (rcsb.org)

The level of myosin light chain (MLC) phosphorylation is an important determinant of smooth muscle contraction. (hindawi.com)

RIOK2 phosphorylation by RSK promotes synthesis of the human small ribosomal subunit. (iric.ca)
Cells expressing the unphosphorylatable mutant (S188A) of Orc2 had defects in DNA synthesis under stress, suggesting that this phosphorylation event is critical to maintain DNA replication under stress. (uky.edu)

Interestingly, targeted mutation of the GluA1 phosphorylation site Ser831 significantly inhibited stress-induced prolongation of incisional pain. (jneurosci.org)
The sequence contains a conserved tyrosine (Y390) whose phosphorylation is induced by agrin and whose mutation abolished clustering of β loop chimeras and their ability to inhibit agrin-induced clustering of the endogenous AChR. (jneurosci.org)

RIOK2 phosphorylation facilitates its release from pre-40S particles and its nuclear re-import, prior to completion of small ribosomal subunits. (iric.ca)
this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (By similarity). (rcsb.org)

Hypoxia is an important micro-environmental factor in promoting tumor progression. (spandidos-publications.com)

In conclusion, Lasp1 facilitated tumor proliferation and invasion of NSCLC through directly binding to FAK and enhancing the phosphorylation of FAK (Tyr397) and AKT (Ser473). (oncotarget.com)

Proliferation and invasion assay showed that Lasp1 dramatically promoted the ability of proliferation and invasion of NSCLC cells. (oncotarget.com)

Phosphorylation of the AChR β subunit is correlated with increased rapsyn/AChR binding, suggesting that the effect of βY390 phosphorylation on clustering is mediated by rapsyn. (jneurosci.org)

A prominent example for the decisive impact of lipid rafts on signaling is LRP6, whose localization to the same lipid rafts domain as the kinase CK1γ is crucial for its successful phosphorylation and the subsequent activation of the signalosome, hence WNT/β-catenin signaling. (frontiersin.org)

PIK3CA/p110α phosphorylation at Y317 and Y508 play important role in tumorigenesis of colorectal cancer through two independent pathways. (biomedcentral.com)
Here, we find graded ERK phosphorylation correlating with cell differentiation in patient-derived colorectal cancer organoids with and without KRAS mutations. (researchgate.net)

Thus, Clb5 and Clb2 use distinct mechanisms to enhance the phosphorylation of S-phase and M-phase substrates. (nature.com)
Consequently, PP2A can increase the activity of caspase 3 by dual mechanisms and thereby promote the apoptotic response in human neutrophils. (lu.se)
Various mechanisms, including endothelial cell nitric oxide and prostacyclin, promote blood fluidity by preventing platelet aggregation and dilating intact blood vessels. (msdmanuals.com)

Lasp1 (LIM and SH3 domain protein 1) promotes tumor proliferation and invasion in multiple cancer entities including non-small cell lung cancer (NSCLC). (oncotarget.com)

Lastly, hydroquinone and NU7026 exhibited synergistic effects on promoting apoptosis by increasing the protein levels of pro-apoptotic proteins Bax and caspase-3 but decreasing the protein expression of anti-apoptotic protein Bcl-2. (cdc.gov)

Moreover, hydroquinone increased the phosphorylation of Akt to activate Akt, whereas co-treatment with NU7026 prevented the activation of Akt by hydroquinone. (cdc.gov)

Subsequent western blot results revealed that Lasp1 promoted the expression of Cyclin A2, CyclinB1, and Snail, and inhibited the expression of E-cadherin. (oncotarget.com)

She has published over 70 research articles, has edited two books ('Quantitative Proteomics' published by RSC Press and 'Histidine Phosphorylation: Methods and Protocols published by Springer Press ) and she currently holds two patents. (wikipedia.org)

To dissect the mechanism pertinent to this observation, we showed that Orc2-S188 phosphorylation associates with DNA replication origin and that cells expressing Orc2-S188A mutant fail to maintain the functional pre-replicative complex (pre-RC) under DNA replication stress. (uky.edu)
Furthermore, we show expression of MSTN by CML mesenchymal stromal cells, and that myostatin propeptide has a direct and instant effect on CML cells, independent of myostatin, by demonstrating binding of myostatin propeptide to the cell surface and increased phosphorylation of STAT5 and SMAD2/3. (haematologica.org)

The results in this dissertation also demonstrate that the protein phosphatase type 2A (PP2A) can directly and independently decrease the phosphorylation levels of both p38 and caspase 3. (lu.se)

Here we compare the specificity of two budding yeast cyclins, the S-phase cyclin Clb5 and the M-phase cyclin Clb2, in the phosphorylation of 150 Cdk1 (Cdc28) substrates. (nature.com)

GLI transcription factor phosphorylation is inhibited by interaction of PRKACA with SMO which sequesters PRKACA at the cell membrane (By similarity). (rcsb.org)
Wnt/β-catenin signaling acts cell-autonomously to promote cardiomyocyte regeneration in the zebrafish heart. (uni-ulm.de)
Mitochondria are also the integrators of stress signals that promote cell death. (upstate.edu)
The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. (cancerindex.org)

Furthermore, palmitoylcarnitine or acetyl-CoA, the primary and final products of Cpt1-mediated fatty acid oxidation, also promoted reprogramming. (biomedcentral.com)

Reduction of 5'-nucleotidase expression or activity may promote metabolic flexibility in type 2 diabetes. (diva-portal.org)
RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). (rcsb.org)

However, phosphorylation sites of PIK3CA/p110α and their underlying mechanism in tumorigenesis are largely unknown. (biomedcentral.com)
Either Y317 or Y508 phosphorylation is essential for tumorigenesis of CRC. (biomedcentral.com)

Anatomy25

Organisms9

Chemicals and Drugs159

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes45

Information Science3

Oxidative phosphorylation7

Tyrosine phosphorylation10

Signaling pathway3

Cyclin-depend3

Inhibition1

Degradation4

Receptor15

Serine phosphorylation2

Protein phosphorylation4

Inhibits1

Kinases2

Decreases1

Substrate2

Regulates3

Acetylation1

Dephosphorylation2

Induces1

Kinase activity1

Overexpression1

Dysfunction1

Inactivation1

PI3K2

MTORC11

Stimulates3

Determinant1

Synthesis2

Mutation2

Nuclear2

Tumor progression1

Ser4731

Assay1

Subunit1

Localization1

Colorectal cancer2

Mechanisms3

Proliferation1

Proteins1

Activation1

Subsequent1

Methods1

Cells2

Decrease1

Substrates1

Cell4

Fatty1

Activity2

Tumorigenesis2