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  • cytoplasm
  • However, if the researchers treated human SH-SY5Y neuroblastoma cells with fibrils of either TDP-43 or FUS, TDP-43 drained out of the nucleus and accumulated in liquid droplets in the cytoplasm. (alzforum.org)
  • Taken together, the results demonstrate that independently of stress granules, TDP-43 phase-separates in the cytoplasm in response to various stressors, and that this can both interfere with nucleocytoplasmic transport and siphon away nuclear TDP-43, resulting in cell death. (alzforum.org)
  • Chook and Shorter wondered if a tightly bound NIR protein might affect the ability of proteins like FUS to undergo phase transition in the cytoplasm. (alzforum.org)
  • occurs
  • In the literature it is generally agreed that successful coalescence of agglomerates occurs only when the agglomerates have excess surface liquid, making the surface plastically deformable. (pharmacologicalsciences.us)
  • behavior
  • The phase behavior of a synthetic phospho-polymethacrylate analog of the Pc3 phosphoproteins, in the presense of Mg( II ), was characterized to determine whether it is chemically possible for the natural adhesive components to be packaged and stored as liquid complex coacervates. (rsc.org)
  • irreversible
  • The disadvantages of these methods include irreversible adsorption of the sample onto the solid matrix, deactivation and/or contamination of the stationary phase, poor sample recovery, and difficulty in scaling up the production of the samples [ 15 ]. (hindawi.com)
  • different
  • Investigators showed that the structure of NPM1 lets it bind to a wide variety of proteins as well as to RNA in different, specific ways that promote phase separation and retain NPM1 and other proteins in the nucleolus. (phys.org)
  • Dormann and her colleagues went on to show that, in ALS and FTD, these phase changes of FUS are promoted in two different ways. (eurekalert.org)
  • further comprising separating the sample components utilizing the different residence time or the unique position of each component in the separation channel. (google.com)
  • structures
  • Borrowing knowledge from fields such as physical chemistry and soft matter physics-where theories explaining liquid-liquid phase separation have informed the development of products ranging from stabilizers in processed foods to cosmetics, from therapeutic ointments to paints-biologists are now developing a new understanding of the nearly two dozen types of membraneless structures characterized so far. (the-scientist.com)
  • nuclear
  • Four papers in the April 19 Cell reveal that nuclear import receptors (NIRs) potently inhibit and even reverse phase transition and fibril formation of several pathogenic RBPs. (alzforum.org)
  • Eftekharzadeh thinks Nup98 might promote the formation of tangles and compromise the nuclear pore complex in the process. (alzforum.org)
  • molecular
  • These molecular networks are essential for phase separation as well as retention of NPM1 and other proteins in the nucleolus and ribosome assembly. (phys.org)
  • Previous work showed that the unfolding of NPM1 may be one of the molecular mechanisms involved in dismantling the liquid-like structure of the nucleolus during cell division. (phys.org)
  • mobile phase
  • 4. Method according to claim 2 further comprising applying an additional force to transport the mobile phase. (google.com)
  • 8. Method according to claims 1 further comprising increasing the residence time of the sample in the separation channel by moving the retentive layer and the mobile phase with respect to a detection point in a mutually opposite direction. (google.com)
  • Baseline separation of the three hormones was achieved using a mobile phase consisting of 1% acetic acid and acetonitrile (75:25, v/v) at pH 4.0. (usda.gov)
  • results
  • The results presented illustrate that the content of the three plant hormones depended on fruit type, fermentation time, and also the number of microorganisms present in the liquid biofertilizers. (usda.gov)
  • reverse phase
  • The method involved sample cleanup using a sep-pack Oasis®MAX cartridge containing mixed-mode anion-exchange and reverse phase sorbents that provided optimum recovery of 85.6%, 91.9%, and 94.3%, respectively, for the three hormones IAA, GA3, and ABA. (usda.gov)
  • analysis
  • The present invention relates to a new separation method and a device therefor, especially to a device and method for chromatographic chemical analysis. (google.com)
  • Method
  • 3. Method according to claim 2 further comprising selecting a motion velocity of the moving channel element to effect a continuous separation. (google.com)
  • 10. Method according to claim 1 further comprising varying the channel temperature during the separation. (google.com)
  • 11. Method according to claim 1 further comprising imposing one or more spatial temperature gradients within the separation channel. (google.com)
  • The advantage of this method is that there exists more accurate liquid dispensing system for precise volume delivery of low viscosity suspensions. (google.com)
  • interface
  • An LED die is mounted onto a stage and a nano-liter pipette is arranged such that an interface separation of approximately 1 millimeter is achieved between the tip of the pipette and the surface of the LED die. (google.com)