Promoter upstream stimulatory factors. Medical search. Frequent questions

The transcription factors, stimulatory proteins 1 and 3 (Sp1 and Sp3), nuclear factor Y (NF-Y), upstream stimulatory factor (USF) and sterol regulatory element binding protein-1 (SREBP-1) have cognate binding sites on the promoter of the FAS gene. (hw.ac.uk)
Ear2 is an orphan nuclear hormone receptor that belongs to the chicken ovalbumin upstream promoter-transcription factors (COUP-TF) gene family [ 14 ]. (biomedcentral.com)
Binding of sequence-specific transcription factors and associated chromatin-modifying enzymes can induce post-translational modification of histone tails and can facilitate nucleosome removal [ 3 - 5 ], which can turn functional sequences such as promoters and enhancers into active, nucleosome-depleted sites [ 2 ]. (biomedcentral.com)
Enhancers promote transcription by providing a binding platform for transcription factors [ 12 ] that can act on (distal) target genes through three-dimensional chromatin looping [ 13 - 15 ]. (biomedcentral.com)
Biochemical studies suggest that in one case this transcription factors to implement particular genetic programs. (lu.se)
In occurs through the inhibition of DNA binding of cognate cis- hematopoiesis there exist several lineage branch points with regulatory motif while in the other case DNA binding is unaffected identified key transcription factors and external signals [3-5]. (lu.se)

Furthermore, it has been demonstrated that the binding of USF1 to the Chitinase 3-Like 1 (Chi3L1) promoter region can enhance the transcriptional activity of Chi3L1. (spandidos-publications.com)
Damcott CM , Feingold E, Moffett SP, Barmada M, Marshall JA, Hamman RF, Ferrell RE (2003) Variation in the FABP2 promoter alters transcriptional activity and is associated with body composition and plasma lipid levels. (umaryland.edu)
Luciferase assay was used to determine the rhBMP-6 or δEF1 driven transcriptional activity of the E-cadherin promoter in MDA-MB-231 cells. (biomedcentral.com)

pSTAT3 in turn cooperates with RelA/NF-κB by binding specific sequences within the proximal promoter of human IL-17A gene, thus inducing its expression. (pasteur.fr)
Quantitative CHIP assay was used to detect the direct association of δEF1 with the E-cadherin proximal promoter in MDA-MB-231 cells. (biomedcentral.com)

Factors mandating cellular consequence include cell surface-receptors, cytoplasmic proteins, and nuclear proteins. (janechin.net)
Protein kinase A can also phosphorylate specific proteins that bind to promoter regions of DNA, causing increases in transcription. (wikipedia.org)
In 1998 a family of cAMP-sensitive proteins with guanine nucleotide exchange factor (GEF) activity was discovered. (wikipedia.org)
Proteins that act both at the cell surface and intracellularly will be discussed in this thesis, with particular emphasis on the fibroblast growth factors. (beauty104.com.tw)
Recently, we elucidated the developmental role of tobacco ( Nicotiana tabacum L.) NtTTG2 in association with 12 genes that putatively encode AUXIN RESPONSIVE FACTOR (ARF) proteins, including NtARF8, NtARF17, and NtARF19. (biomedcentral.com)

It was also demonstrated that USF1 directly binds to the promoter region of 2,492 genes, which may be involved in the viral progression and cell proliferation pathways. (spandidos-publications.com)
Promoter region motif analysis indicated that ETV5 has more binding motifs and genes than USF1 itself for USF1‑regulated DEGs, indicating that USF1 may indirectly modulate gene expression by regulating ETV5 expression in Huh7 cells. (spandidos-publications.com)
In summary, the results demonstrated that USF1 binds to the promoter region of thousands of genes and affects a large part of DEGs indirectly. (spandidos-publications.com)
As a TF, USF1 has a bidirectional regulatory function, being able to regulate gene expression by activating or suppressing the promoter region of target genes ( 5 , 6 ). (spandidos-publications.com)
Following on from the achievements in molecular studies of monogenic disorders, recent studies have used strategies of hypothesis-free fine mapping of genes and loci to identify underlying factors in common complex diseases with major impacts on public health. (biomedcentral.com)
These diseases, which include cancers, coronary heart disease, schizophrenia, autism and multiple sclerosis, arise from complex interactions between environmental factors and variation in several different genes. (biomedcentral.com)
Maternal THs acted upstream of pax2a, pax7 and pax8 genes but downstream of shha and fgf8a signalling. (pharmaceuticalintelligence.com)
To exert stimulatory or repressive effects on transcription, accessible regulatory DNA elements must be in close spatial proximity to susceptible genes. (biomedcentral.com)
The latter pair inherits the state of its upstream master genes and further reinforces the decision due to several feedback loops, thereby leading to irreversible commitment. (lu.se)

The activity of the adenovirus major late promoter is substantially Increased as the infection proceeds from the early to late phase. (princeton.edu)

Little or no protein interaction at promoter sequences was detected early (5 hr) after infection but strong interactions at the major late transcription factor (MLTF/USF) binding site and at the TATA box were evident late (12 hr) after infection. (princeton.edu)
Glucose and GLP-1 also synergistically activated NFAT (nuclear factor of activated T-cells)-mediated transcription from a minimal promoter construct containing tandem NFAT consensus sequences. (diabetesjournals.org)
Two potential promoters and two repeat sequences (RS, 16 bp), spaced to each other by 1661 bp, were also found upstream of the βhsd gene C. testosteroni. (idexlab.com)
Our data reveal that the βhsd gene undergoes complex regulation involving the two promoters, a loop structure via the two repeat sequences, and the steroid Testosterone . (idexlab.com)

Upstream stimulatory factor 1 is a protein that in humans is encoded by the USF1 gene. (wikipedia.org)
Upstream transcription factor 1 (USF1) is a canonical transcription factor (TF) and is associated with the pathogenesis of several cancers, but its biological functions and molecular targets in HCC remain unclear. (spandidos-publications.com)
The study also validated the direct interaction between USF1 and the promoter of ETV5 using ChIP‑qPCR. (spandidos-publications.com)
For instance, a previous study by the authors validated that USF1 binds to the core promoter of APOBEC3G and increases its transcription level in hepatocytes ( 7 ). (spandidos-publications.com)

To gain insight into the regulation of this promoter, we analyzed protein-DNA interactions by in vivo DMS and DNasel footprinting during the course of adenovirus infection. (princeton.edu)
We have previously identified NFAT (nuclear factor of activated T-cells) as a key regulator of insulin gene transcription in pancreatic β-cells that is activated by the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin) in response to increased [Ca 2+ ] i ( 10 ). (diabetesjournals.org)
[1] Adenylate cyclase is activated by a range of signaling molecules through the activation of adenylate cyclase stimulatory G ( G s )-protein-coupled receptors. (wikipedia.org)
In this study we used phosphorylation of the transcription factor cAMP response element-binding protein as a functional readout to identify cells responding to EGF and FGF-2. (beauty104.com.tw)
An unusual cellular factor potentiates protein-DNA complex assembly between Oct-1 and Vmw65. (mcmaster.ca)
Upon examining the genome with bioinformatics tools, a gene (brp) coding for a regulator protein (BRP) for 3,17β-HSD expression was found upstream of the βhsd gene. (idexlab.com)

Gefitinib and erlotinib would be effective for tumors with epidermal growth factor receptor (EGFR) mutations or increased EGFR gene copy numbers ( 6 ). (aacrjournals.org)
Homeostatic osteoclastogenesis is effectively induced by RANKL (Receptor activator of nuclear factor kappa-B ligand). (frontiersin.org)
CD28 is an important co-stimulatory receptor for T lymphocytes that, in humans, delivers TCR-independent signal leading to the up-regulation of pro-inflammatory cytokines. (pasteur.fr)
Growth factors that signal through tyrosine-kinase receptor families include the epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and transforming-growth factor-α (TGF-α). (janechin.net)
Transforming growth factor-β1 (TGF-β) signals through a serine/threonine-kinase receptor pathway. (janechin.net)
This can either occur by cell surface receptor interactions, as in the case with peptide growth factors, or by interactions with intracellular molecules, as in the case with steroid hormones. (beauty104.com.tw)

Association of an Insulin Gene Promoter Insertion Polymorphism with Type 2 Diabetes and Diabetic Nephropathy in African Americans. (uky.edu)
The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. (nih.gov)

Nuclear extracts prepared from uninfected cells as well as cells harvested at 5 and 12 hr after infection contained similar levels of MLTF/USF footprint activity, therefore the lack of a detectable interaction early after infection is not due to reduced levels of the factor early in the viral growth cycle. (princeton.edu)

Here we show that CD28-mediated up-regulation of IL-17A gene expression depends on RelA/NF-κB and IL-6-associated STAT3 transcriptions factors. (pasteur.fr)
Finally, by using specific inhibitory drugs, we also identified class 1A phosphatidylinositol 3-kinase (PI3K) as a critical upstream regulator of CD28-mediated RelA/NF-κB and STAT3 recruitments and trans-activation of IL-17A promoter. (pasteur.fr)
Lastly, IL-21 also promotes activation of the STAT3 promoter and other transcriptional factors. (intechopen.com)

a cis-acting sequence that increases the utilization of (some) eukaryotic promoters, and can function in either orientation and in any location (upstream or downstream) relative to the promoter. (insdc.org)

Variation Within the Gene Encoding the Upstream Stimulatory Factor 1 Does Not Influence Susceptibility to Type 2 Diabetes in Samples from Populations with Replicated Evidence of Linkage to Chromosome 1q. (uky.edu)
Insulin Promoter Factor 1 Variation is Associated with Type 2 Diabetes in African Americans. (uky.edu)

bFGF is a more potent angiogenic factor than VEGF ( vascular endothelial growth factor ) or PDGF ( platelet-derived growth factor ). (beauty104.com.tw)

Osteoclast differentiation is induced by the master osteoclastogenic factor, RANKL, which acts in concert with M-CSF and ITAM-mediated co-stimulatory signaling. (frontiersin.org)

Epidermal growth factor (EGF) and fibroblast growth factor 2 (FGF-2) induce the proliferation of neural precursor cells isolated from specific regions of the embryonic and adult brain. (beauty104.com.tw)

Antibiotic therapy is the key factor that alters the colonic flora. (medscape.com)

Both glucose and GLP-1 stimulate insulin promoter activity, stabilize proinsulin mRNA, and increase proinsulin biosynthesis ( 14 ). (diabetesjournals.org)

a transcriptional cis regulatory region that when located between an enhancer and a gene's promoter prevents the enhancer from modulating the expression of the gene. (insdc.org)
To characterize the BRP functional DNA domain, different fragments of the βhsd upstream regulatory region were tested in a cotransformation system. (idexlab.com)

These results indicate that DNA replication participates in the regulation of adenovirus late gene expression by facilitating the binding of a transcription factor to the major late promoter. (princeton.edu)
Glucose metabolism is central to the regulation of β-cell function ( 11 ), and GLP-1 is a potent hormonal incretin that has been shown to markedly enhance the stimulatory effects of glucose on β-cells ( 12 , 13 ). (diabetesjournals.org)

This gene encodes a member of the basic helix-loop-helix leucine zipper family and can function as a cellular transcription factor. (wikipedia.org)
Comparison of in vivo and in vitro footprints revealed that the in vivo interaction late after infection results from binding of the cellular transcription factor MLTF/USF. (princeton.edu)
Upstream transcription factor (USF) 1 belongs to the basic helix-loop-helix leucine zipper family and serves as a cellular transcription factor (TF). (spandidos-publications.com)

Viral DNA replication was required for MLTF/USF binding at the major late promoter. (princeton.edu)

Evaluation of Sequence Variants in the Pre-B Cell Leukemia Transcription Factor 1 Gene: A Positional and Functional Candidate for Type 2 Diabetes and Impaired Insulin Secretion. (uky.edu)
However, FGFs 11-14, also known as FGF homologous factors 1-4 (FHF1-FHF4), have been shown to have distinct functional differences compared to the FGFs. (beauty104.com.tw)

Commonalities in cell-specific and/or temporal-specific aspects of gene expression do not appear to be main factors governing alignment within the locus. (biomedcentral.com)

This is consistent with our analysis of the E-cadherin promoter demonstrating that BMP-6 was a potent transcriptional activator. (biomedcentral.com)

Rasd1 inhibited transcriptional repression by Ear2 on a renin promoter-luciferase reporter construct both in the presence and absence of all-trans-retinoic acid. (biomedcentral.com)

TNF (Tumor necrosis factor) is a pleiotropic cytokine that plays an important role in immunity and inflammatory bone destruction. (frontiersin.org)

In this study, GLP-1 (100 nmol/l), in the presence of glucose (11 mmol/l), induced a ∼71-fold increase in insulin gene promoter activity in INS-1 pancreatic β-cells, an effect that was an order of magnitude larger than with either stimulant alone. (diabetesjournals.org)
Soluble suppressor activity obtained from allopregnant mouse decidua that blocks the cytolytic effector response to IL-2 is related to transforming growth factor-beta. (mcmaster.ca)

Although these factors possess remarkably similar sequence homology, they do not bind FGFRs and are involved in intracellular processes unrelated to the FGFs (Olsen et al. (beauty104.com.tw)

Delineation and characterisation of mutations in the factor IX gene of haemophilia B patients. (edu.au)
Furthermore, two-point base pair mutations in any of the three identified NFAT sites within the rat insulin I promoter resulted in a significant reduction in the combined effect of glucose and GLP-1. (diabetesjournals.org)

These factors couple extracellular signals to intracellular processes to determine cell cycle progression. (janechin.net)

Dysregulation of processes driven by these factors is a severe liability to the cell and therefore represents potential areas for therapeutic intervention. (janechin.net)
The second pathway also involves glucose metabolism but appears to be driven by glucose-derived factors that target insulin gene transcription independently of [Ca 2+ ] i . (diabetesjournals.org)

Transforming growth factor-β in cancer therapy (ed. (shoyaku.ac.jp)
Comparative biochemical studies of rapamycin and wortmannin on transforming growth factor-beta 1 (TGF-beta1)-mediated G(1)-S cell cycle progression in C3H-10T1/2 embryonic fibroblasts. (janechin.net)
Knowledge about how growth factors and hormones function is pivotal in order to rationally try to stimulate or inhibit their effects for therapeutical purposes. (beauty104.com.tw)
After growth factor treatment for 6 d, a population of nestin-positive cells responding to both EGF and FGF-2 were identified. (beauty104.com.tw)
Fibroblast growth factors , or FGFs , are a family of growth factors involved in wound healing and embryonic development. (beauty104.com.tw)
Fibroblast growth factor was found in a cow brain extract by Gospodarowicz and colleagues and tested in a bioassay which caused fibroblasts to proliferate (first published report in 1974). (beauty104.com.tw)
They then further fractionated the extract using acidic and basic pH and isolated two slightly different forms that were named 'acidic fibroblast growth factor' (FGF1) and 'basic fibroblast growth factor' (FGF2). (beauty104.com.tw)
Not long after FGF1 and FGF2 were isolated, another group isolated a pair of heparin -binding growth factors which they named HBGF-1 and HBGF-2, whilst a third group isolated a pair of growth factors that caused proliferation of cells in a bioassay containing blood vessel endothelium cells which they called ECGF-1 and ECGF-2. (beauty104.com.tw)
Effect of the Concentration Levels of Growth Hormone and Insulin-like Growth Factor I on the Polymorphisms of the Il12p40 Gene in Lung Cancer Patients. (nih.gov)
Characterization of murine pregnancy decidua transforming growth factor beta. (mcmaster.ca)

However, whereas pK2.4-EGFP-4 could integrate into the chromosomal DNA of C. testosteroni and knock out the βhsd gene promoter, pBB2.4-EGFP-8 could replicate in C. testosteroni cells as a free plasmid DNA. (idexlab.com)
After integration of pK2.4-EGFP-4 into the βhsd gene promoter, 3,17β-HSD expression could not be induced such that EGFP expression in the mutant cells was at low levels. (idexlab.com)

Schloer S, Goretzko J, Pleschka S, Ludwig S, and Rescher U. (2020) Combinatory Treatment with Oseltamivir and Itraconazole Targeting Both Virus and Host Factors in Influenza A Virus Infection. (uni-muenster.de)

However, the IFN-γ-induced IFN regulatory factor-1 (IRF-1) and intercellular adhesion molecule 1 (ICAM-1) expression is not affected, suggesting that chlamydia may selectively target the IFN-γ signaling pathways required for MHC class II expression. (silverchair.com)
Chlamydial inhibition of MHC class II expression is correlated with degradation of upstream stimulatory factor (USF)-1, a constitutively and ubiquitously expressed transcription factor required for IFN-γ induction of class II transactivator (CIITA) but not of IRF-1 and ICAM-1. (silverchair.com)
In addition to down-regulating the expression of δEF1, BMP-6 also physically dislodged δEF1 from E-cadherin promoter to allow the activation of E-cadherin transcription. (biomedcentral.com)

NFAT binds to three distinct NFAT elements within the rat I insulin promoter and activates insulin gene transcription. (diabetesjournals.org)

We found that a 2.4 kb regulatory DNA fragment upstream of the 3,17β-HSD gene (βhsd) responds to steroids and triggers βhsd gene induction. (idexlab.com)

Anatomy8

Diseases2

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

Phenomena and Processes20

Information Science3

Transcription factors6

Transcriptional activity3

Proximal promoter2

Proteins5

Genes9

Adenovirus major late1

Sequences4

USF14

Protein6

Receptor6

Polymorphism2

Nuclear1

STAT33

Downstream1

Variation2

Platelet-derive1

Differentiation1

Epidermal1

Alters1

MRNA1

Regulatory region2

Regulation2

Cellular3

MLTF1

Functional2

Gene expression1

Potent1

Construct1

Cytokine1

Activity2

Bind1

Mutations2

Intracellular1

Driven2

Growth10

Cells2

Infection1

Expression3

Distinct1

Found1