• Retinoids and retinoid analogues exert their activities by interacting with nuclear receptors (RARs [retinoic acid receptors] or RXRs [retinoic X receptors]) on cells and by activating genes that contain RARE (retinoic acid response elements) or RXRE (retinoic X response elements) in their promoters (Michel, Jombard, & Démarchez, 1998). (medscape.com)
  • The AP-1 transcription complex controls the expression of a subset of genes that are expressed early in response to extracellular mitogenic stimuli or to stress. (medscape.com)
  • Our in silico findings report that there is cross-regulation between both pathways at the level of gene transcription regulation, mediated by the presence of binding sites for both factors in promoters of genes essential for these pathways. (biomedcentral.com)
  • In this paper we examine evidence for a direct crosstalk of the NF-κB and IRF3 signaling pathways at the lowest level, between main transcription factors and genes coding for these transcription factors. (biomedcentral.com)
  • We analyzed respective gene promoters' sequences using in silico methods for identification of transcription binding sites, mainly in the NF-κB- and IRF-coding genes. (biomedcentral.com)
  • This study shows that the nucleosome remodeller dMi-2 is recruited to ecdysone-regulated genes to limit transcription. (sdbonline.org)
  • At the cellular level, the actions of glucocorticoids are mediated by a 94-kd protein, the glucocorticoid receptor (GR). The human (h) GR belongs to the steroid/thyroid/retinoic acid superfamily of nuclear receptors and functions as a ligand-dependent transcription factor that regulates the expression of glucocorticoid-responsive genes positively or negatively. (medscape.com)
  • Here, it is demonstrated that TGFβ facilitates Tβ4 expression and leads to the activation of myocardin-related transcription factors (MRTF), which are coactivators of serum response factor (SRF) and regulate the expression of genes critical for the epithelial-mesenchymal transition (EMT) and tumor metastasis. (aacrjournals.org)
  • The DNA-binding domain (DBD) of the hGRα corresponds to amino acids 420-480 and contains 2 zinc finger motifs through which the hGRα binds to specific DNA sequences, the glucocorticoid-response elements (GREs) in the promoter region(s) of target genes. (medscape.com)
  • Dimerization is required for specific binding to a DNA sequence known as the TPA response element (TRE) found in the promoter region of many genes. (biomedcentral.com)
  • 100 genes ( 1 ). (cdc.gov)
  • Conclusion】 The expression trends of ADAMTS 1, PI 3 K and A k t genes and proteins were consistent and showed a positive correlation in ovary and granulosa cells of Mongolian sheep,suggested that ADAMTS 1 gene played an important role in twin birth of Mongolian sheep mediated by PI3K/Akt signaling pathway. (chvm.net)
  • These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
  • 2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)
  • This TF induction was dependent on signal transducer and activator of transcription-6 signaling and poly ADP ribose polymerase activity. (haematologica.org)
  • Stimulation of TLRs (Toll-Like Receptors) by a pathogen induces activation of signal transduction cascades, which leads to translocation of nuclear factor-κB (NF-κB) to the nucleus [ 1 ], activation of interferon regulatory factors 3/7 (IRF3/7) and/or activator protein-1 (AP-1), which cooperate to induce transcription of various cytokines such as alpha/beta interferon (IFN-α/β) to counteract infection [ 2 - 4 ]. (biomedcentral.com)
  • Using gene-silencing experiments and specific pharmacological inhibitors, we have ascertained that EGF and TGFα induce GPR30 expression through the EGFR/ERK transduction pathway, and the recruitment of c-fos to the activator protein-1 site located within GPR30 promoter sequence. (unical.it)
  • Transcription factor binding sites, including that for activator protein (AP)-1, were found in this region. (quantichrome.com)
  • In the present study we provide evidence that the activator protein-1 (AP-1) transcription factor plays a critical role in this mechanoinduction process. (elsevierpure.com)
  • This is an all-inclusive cell-based luciferase reporter assay kit targeting the Human Activator Protein-1 (AP-1). (indigobiosciences.com)
  • INDIGO's AP-1 reporter assay utilizes proprietary mammalian cells that have been engineered to provide constitutive expression of the Human Activator Protein-1 (AP-1). (indigobiosciences.com)
  • In addition to AP-1 Reporter Cells, this kit provides two optimized media for use during cell culture and in diluting the user's test samples, a reference activator, Luciferase Detection Reagent, and a cell culture-ready assay plate. (indigobiosciences.com)
  • Activator protein-1 (AP-1) and nuclear factor-kB (NF-kB) have been shown to play pivotal roles in tumor initiation, promotion, and progression. (cdc.gov)
  • Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)
  • Hepatocyte growth factor receptor (HGF receptor) is a protein that in humans is encoded by the MET gene. (wikipedia.org)
  • 2004. Cloning of the rat beta-catenin gene (Ctnnb1) promoter and its functional analysis compared with the Catnb and CTNNB1 promoters. . (oregonstate.edu)
  • Additionally, they can regulate gene expression by inhibiting the activity of other transcription factors, such as AP-1. (medscape.com)
  • Responsiveness of the α5 gene toward collagen was determined by transfection of recombinant plasmids bearing the CAT reporter gene under the control of various segments from the human α5 promoter into primary cultured rabbit (RCECs) and human (HCECs) CECs cultured on BSA or on collagens. (nih.gov)
  • Electrophoretic mobility shift assays (EMSAs) and Western blot analyses were used to monitor both the DNA binding and expression of the transcription factors required to ensure basal transcription of the α5 gene. (nih.gov)
  • These collagen-dependent regulatory influences also correlated with alterations in either the expression or the DNA binding of the transcription factors Sp1/Sp3, NFI and AP-1 that ensure basal transcription of the α5 gene. (nih.gov)
  • RA, but not these 'dissociating' retinoids, induced transcription of an interleukin-6 promoter-based reporter gene transiently transfected into HeLa cells together with RARs. (nih.gov)
  • TP53 (p53) binds to the p53 response element in the promoter of the MSH2 gene. (reactome.org)
  • In multiple human cancers, Tβ4 levels correlate positively with TGFβ1 and the tumor-associated gene expression levels through processes that respectively depend on TGFβ receptor 1 (TGFBR1) and MRTF expression. (aacrjournals.org)
  • Transcription factor involved in regulating gene activity following the primary growth factor response. (abcam.cn)
  • The 5′-flanking region of the Sema3A gene was cloned, and a critical region for Sema3A promoter activity within -134 bp of the start codon was identified. (quantichrome.com)
  • We have isolated a genomic clone of the human TIMP-1 gene. (uea.ac.uk)
  • Constructs of -738/+95 to -194/+21 are inducible with serum or phorbol ester to a similar extent to the endogenous TIMP-1 gene. (uea.ac.uk)
  • These results and further mapping with 5' deletion mutants from the -738/+95 region have demonstrated that an AP-1 site at -92/-86 is essential for basal expression of the gene. (uea.ac.uk)
  • Currently there is intense interest to define the mechanism of action of glucagon-like peptide-1 (GLP-1) in regulating β-cell function, including insulin gene transcription. (diabetesjournals.org)
  • In this study, GLP-1 (100 nmol/l), in the presence of glucose (11 mmol/l), induced a ∼71-fold increase in insulin gene promoter activity in INS-1 pancreatic β-cells, an effect that was an order of magnitude larger than with either stimulant alone. (diabetesjournals.org)
  • These data suggest that the synergistic action of glucose and GLP-1 to promote insulin gene transcription is mediated through NFAT via PKA- and calcineurin-dependent pathways in pancreatic β-cells. (diabetesjournals.org)
  • The rate of insulin gene transcription in pancreatic β-cells is regulated by a complex integration of signals derived from nutrients, hormones, and neurotransmitters ( 1 - 5 ). (diabetesjournals.org)
  • We have previously identified NFAT (nuclear factor of activated T-cells) as a key regulator of insulin gene transcription in pancreatic β-cells that is activated by the calcium/calmodulin-dependent protein phosphatase 2B (calcineurin) in response to increased [Ca 2+ ] i ( 10 ). (diabetesjournals.org)
  • NFAT binds to three distinct NFAT elements within the rat I insulin promoter and activates insulin gene transcription. (diabetesjournals.org)
  • Two signaling pathways arising from glucose metabolism converge to activate NFAT-mediated insulin gene transcription. (diabetesjournals.org)
  • One pathway results from the direct effect of increased [Ca 2+ ] i , which activates calcineurin, and in turn, it upregulates insulin gene transcription via NFAT. (diabetesjournals.org)
  • The second pathway also involves glucose metabolism but appears to be driven by glucose-derived factors that target insulin gene transcription independently of [Ca 2+ ] i . (diabetesjournals.org)
  • The stretch response of the MMP-1 gene was also completely inhibited by curcumin. (elsevierpure.com)
  • Collectively, these findings indicate that AP-1-mediated transcription plays an important role in the regulation of gene expression in bladder muscle in response to mechanical forces. (elsevierpure.com)
  • Figure 1: Summary of estrogen receptor and RNA PolII binding sites and correlation with nucleotide and gene number. (nature.com)
  • Reporter Cells contain an engineered luciferase reporter gene functionally linked to tandem TPA response element (TRE) sequences positioned immediately upstream of a minimal promoter. (indigobiosciences.com)
  • The present study examines effects of metallic nickel fine and nanoparticles on tumor promoter or suppressor gene expressions as well as on cell transformation in JB6 cells. (cdc.gov)
  • Conclusion】 The PERP 1 gene had the function of promoting granulosa cell apoptosis,and regulating its expression was of great significance for follicular development. (chvm.net)
  • Objective】 This study was aimed to analyze the expression pattern of a disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAMTS1) on PI3K/Akt pathway in ovary of twin and singleton Mongolian sheep at different stages of estrous cycle,and explore the mechanism of ADAMTS 1 gene influencing reproductive traits in Mongolian sheep. (chvm.net)
  • Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
  • Classical polarization is usually associated with a proinflammatory response, including the increased production of tumor necrosis factor (TNF)-α, IL-1, and IL-6. (haematologica.org)
  • Coronel L , Riege K , Schwab K , Förste S , Häckes D , Semerau L , Bernhart SH , Siebert R, Hoffmann S , Fischer M . Transcription factor RFX7 governs a tumor suppressor network in response to p53 and stress. (mitteldeutsches-krebszentrum.de)
  • Here we have investigated nc886 in gastric cancer where its expression is suppressed due to CpG DNA hypermethylation at its promoter region in a cohort of paired tumor/normal tissues from 88 gastric cancer patients. (oncotarget.com)
  • this fragment contains 1718 bp 5' flanking sequences, exon 1, a 929 bp intron 1 and part of exon 2. (uea.ac.uk)
  • Computer analysis reveals 10 consensus sequences for Sp1, six for activating protein 1 (AP-1), six for polyoma enhancer A3 (PEA3), 12 for AP-2 and five CCAAT boxes. (uea.ac.uk)
  • Glucose and GLP-1 also synergistically activated NFAT (nuclear factor of activated T-cells)-mediated transcription from a minimal promoter construct containing tandem NFAT consensus sequences. (diabetesjournals.org)
  • Several transcription regulatory sequences were identified in the promoter region. (elsevierpure.com)
  • In addition to a TATA box at position -29 relative to the transcription initiation site and two Spl GGGCGG recognition sequences at nucleotide positions -42 to -37 and -50 to -45, the promoter contains a sequence motif matching the transcription activating factor AP-1. (elsevierpure.com)
  • The AP-1 transcription factor complex binds the MSH2 promoter cooperatively with TP53 (Scherer et al. (reactome.org)
  • Gel-shift analysis shows that protein binds specifically to this region, but competition studies suggest that it is unlikely to be LBP-1. (uea.ac.uk)
  • Both the overexpression of Met/HGFR, as well as its autocrine activation by co-expression of its hepatocyte growth factor ligand, have been implicated in oncogenesis. (wikipedia.org)
  • Notably, the separate overexpression of EcR-B1 or a transforming growth factor- β receptor Baboon , whose activation can upregulate the EcR-B1 expression, in MB neurons rescued the miR-34 -induced gamma axon pruning phenotype. (sdbonline.org)
  • The NTD of the hGRα contains a major transactivation domain, termed activation function (AF)-1, which is located between amino acids 77 and 262 of the hGRα and is ligand-independent. (medscape.com)
  • Transient overexpression of UBCv1 impaired activation of NF-κB and AP-1 transcription factors induced by several agonists of these pathways. (mdpi.com)
  • Targets transcription factors involved in the regulation of inflammatory responses and in the immune response: suppresses NF-kappa-B activation, and activates AP-1 (By similarity). (proteopedia.org)
  • Consistent with the promoter and gel-shift studies, curcumin, an inhibitor of AP-1 activation, suppressed the HB-EGF mRNA induction after stretch. (elsevierpure.com)
  • Dose-response activation of AP-1 using PMA. (indigobiosciences.com)
  • Our results demonstrate that metallic nickel nanoparticles caused higher activation of AP-1 and NF-kB, and a greater decrease of p53 transcription activity than fine particles. (cdc.gov)
  • Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)
  • Tiling a given promoter region with several sgRNAs can produce more robust transcriptional activation 9 - 11 , but this requirement presents enormous challenges for scalability, and in particular for establishing pooled, genome-wide GOF screens. (cdc.gov)
  • Th2 cells have a differentiation process which is dependent on IL-4, which causes the activation of the transcription factor STAT 6 . (bvsalud.org)
  • As the material increased in purity, there are more high-aspect-ratio particulates and a corresponding distinct increase in cytotoxicity, nuclear factor-B transcription, and inflammasome activation. (cdc.gov)
  • These results illustrate the combined impact of genetic, lifestyle, and environmental factors in modulating immunological responsivity and emphasize the importance of the exposome in shaping biological responses. (cdc.gov)
  • This study aims to clarify the time-dependent molecular pathways by which TMAO mediates endothelial dysfunction through transcriptomics and metabolomics analyses in human microvascular endothelial cells (HMEC-1). (nature.com)
  • However, the KEGG signalling pathways namely "tumour necrosis factor (TNF)" and "cytokine-cytokine receptor interaction" were downregulated at 24H but activated at 48H. (nature.com)
  • Our results emphasize the need for new endocrine agents able to block widespread actions of estrogen without exerting any stimulatory activity on transduction pathways shared by the steroid and growth factor-signaling networks. (unical.it)
  • AP-1 is one of the transcription factors found at the receiving end of multiple signaling pathways. (biomedcentral.com)
  • An operating TATA container was determined in ITGB6 promoter upstream from the transcription-initiation site (TSS). (researchatlanta.org)
  • We demonstrate that epidermal growth factor (EGF) and TGFα transactivate the GPR30 promoter and accordingly up-regulate GPR30 mRNA and protein levels only in endometrial and tamoxifen-resistant breast cancer cells. (unical.it)
  • Both glucose and GLP-1 stimulate insulin promoter activity, stabilize proinsulin mRNA, and increase proinsulin biosynthesis ( 14 ). (diabetesjournals.org)
  • Stretch also specifically increased mRNA levels for matrix metalloproteinase (MMP)-1, the promoter of which contains a functional AP-1 element, but not for MMP-2, the promoter of which does not contain an AP-1 element. (elsevierpure.com)
  • 2000). An additional p53 response element in closer proximity to the MSH2 transcription start site has been reported that does not involve a nearby AP-1 binding site (Warnick et al. (reactome.org)
  • 2000). We of glial cell line-derived neurotrophic factor (GDNF) in models of have recently reported that this vector directs transgene expression to Parkinson's disease (Georgievska et al. (lu.se)
  • Objective】 The purpose of this study was to investigate the regulatory role and expression mechanism of TP53 apoptotic effector factor (PERP1) in follicular development. (chvm.net)
  • Topical retinoids also demonstrate inhibition of various immune factors, including the activity of leukocytes, the release of pro-inflammatory cytokines and other mediators, and the expression of transcription factors and toll receptors involved in immunomodulation (Wolf, 2002). (medscape.com)
  • This study examined the roles of hormone-dependent transcription factors (Nuclear Receptors), in accessory gland development, function and male fertility of a genetically tractable insect model, Drosophila melanogaster. (sdbonline.org)
  • APOE associated to the LVP allows the initial virus attachment to cell surface receptors such as the heparan sulfate proteoglycans (HSPGs), syndecan-1 (SDC1), syndecan-1 (SDC2), the low-density lipoprotein receptor (LDLR) and scavenger receptor class B type I (SCARB1) (PubMed:12970454, PubMed:12356718, PubMed:12913001, PubMed:28404852, PubMed:22767607). (proteopedia.org)
  • In contrast to monocytes, human macrophages did not show increased tissue factor expression upon stimulation with lipopolysaccharide and interferon-γ. (haematologica.org)
  • Using 5′ RACE, we identified three transcription start sites and several splice variants of ChemR23 in both monocytes and macrophages. (aai.org)
  • Activated IRF3, together with NF-κB and AP-1, accumulate in the nucleus, bind to the IFNβ promoter, and stimulate transcription. (cdc.gov)
  • The inability of proinflammatory activated macrophages to respond to lipopolysaccharide and interferon-γ with an increase in TF production seems to be due to an increase in TF promoter methylation and was reversible when treating these macrophages with a demethylation agent. (haematologica.org)
  • The present study investigated the methylation of CpG sites in the cyclooxygenase (COX)‑2 promoter via nuclear factor (NF)‑κB transcriptional regulation and elucidated its effect on the COX‑2 transcriptional expression in a ketamine‑induced ulcerative cystitis (KIC) animal model. (spandidos-publications.com)
  • Moreover, in the ketamine group, the level of Ten‑Eleven‑Translocation methylcytosine dioxygenase for demethylation as determined by reverse transcription‑quantitative PCR assay was increased in comparison with the control group, but that was not the case for the level of DNA methyltransferases for methylation. (spandidos-publications.com)
  • The mechanism of epigenetic regulation involves the CpG site methylation of promoter regions and the modification of DNA and histones by altering chromatin structure ( 9 - 11 ). (spandidos-publications.com)
  • Transient transfection of promoter-chloramphenicol O-acetyltransferase reporter constructs into primary human connective tissue fibroblasts shows that a 904 bp fragment that hybridizes to a murine TIMP-1 promoter fragment contains a functional promoter. (uea.ac.uk)
  • AP-1 is activated by a variety of physiological and environmental stimuli such as growth factors, cytokines, stress, ultraviolet radiation, and bacterial and viral infections. (indigobiosciences.com)
  • H)-1 and TH2 cytokines have been demonstrated in the airways of subjects with different asthma phenotypes, reported causes of occupational asthma in the work-place. (cdc.gov)
  • The region hybridizing with a murine TIMP-1 promoter fragment has been subcloned and analysed further. (uea.ac.uk)
  • Hepatocyte growth factor/Scatter Factor (HGF/SF) and its splicing isoform (NK1, NK2) are the only known ligands of the HGF receptor. (wikipedia.org)
  • The AF-1 plays an important role in the interaction of the receptor with molecules necessary for the initiation of transcription, such as coactivators, chromatin modulators, and basal transcription factors, including RNA polymerase II, TATA-binding protein (TBP), and a host of TBP-associated proteins (TAFIIs). (medscape.com)
  • E1/E2 heterodimer binding on CD81 activates the epithelial growth factor receptor (EGFR) signaling pathway (PubMed:22855500). (proteopedia.org)
  • Figure 4: Identification of enriched motifs within the estrogen receptor binding sites and validation of transcription factor binding. (nature.com)
  • Figure 5: Involvement of cooperating transcription factors at estrogen receptor binding sites. (nature.com)
  • Estrogen receptor-alpha directs ordered, cyclical, and combinatorial recruitment of cofactors on a natural target promoter. (nature.com)
  • Mouse monoclonal to CD64.CT101 reacts with high affinity receptor for IgG (FcyRI), a 75 kDa type 1 trasmembrane glycoprotein. (researchatlanta.org)
  • All collagen types repressed the transcriptional activity directed by the full-length α5 promoter/CAT construct in confluent CECs. (nih.gov)
  • The main challenge for large bone defect repair and regeneration remains the inadequate recruitment of mesenchymal stem cells (MSCs), reduced vascularization, and decreased growth factors stimulation within the scaffold construct to support cell viability and tissue growth. (frontiersin.org)
  • Rat bladder SMC were transiently transfected with a series of 5' deletion mutants of a promoter-reporter construct containing 1.7 kb of the mouse HB-EGF promoter that was previously shown to be stretch responsive. (elsevierpure.com)
  • The human GR is a modular protein composed of distinct regions illustrated in panel B in the image below, as follows: (1) The amino-terminal A/B region, also called immunogenic or N-terminal domain (NTD) and (2) the C, D, and E regions, which correspond to the DNA-binding domain, the hinge region, and the ligand-binding domain, respectively. (medscape.com)
  • Furthermore, this resource has allowed the identification of cis -regulatory sites in previously unexplored regions of the genome and the cooperating transcription factors underlying estrogen signaling in breast cancer. (nature.com)
  • Formation of Activated Protein 1 (AP-1) complex. (reactome.org)
  • The calcium-mediated transient up-regulation of Sema3A expression was significantly suppressed by mitogen-activated protein kinase [ MAPK ]/extracellular signal-regulated kinase [ERK] ( MEK ) 1/2 or AP-1 inhibitors. (quantichrome.com)
  • The +21/+58 region contains a putative binding site for the transcription factor leader-binding protein 1 (LBP-1). (uea.ac.uk)
  • The response to GLP-1 was mimicked by forskolin and largely inhibited by the protein kinase A (PKA) inhibitors, H89 and myristoylated PKI(14-22) amide, indicating partial mediation via a cAMP/PKA pathway. (diabetesjournals.org)
  • The c-Jun dimerization protein 2, JDP2 is an AP-1 repressor protein that potently inhibits AP-1 transcription. (biomedcentral.com)
  • Most of the important insights regarding the specific function of AP-1 proteins have been established through the use of mice with either loss- or gain-of-function manipulations of various members of the bZIP protein family [ 13 ]. (biomedcentral.com)
  • In influenza viruses, the nonstructural protein 1 inhibits the interferon-mediated antiviral response. (cdc.gov)
  • Binding to dsRNA by these proteins may result in initiation of helicase activity, concomitant with a conformational change that leads to recruiting additional cellular factors, including the recently identified IPS-1/MAVS protein ( 5 , 6 ). (cdc.gov)
  • Here we describe a different method of transgene regulation by the use of the human glial ®brillary acidic protein (GFAP) promoter. (lu.se)
  • Deletions from the 3' end also implicate a region across the exon 1/intron 1 boundary and especially +21 to +58 in basal expression. (uea.ac.uk)
  • Binding of AP-1, as evaluated by electrophoretic mobility shift assay, to a synthetic oligonucleotide containing an AP-1 binding site increased in response to stretch, and binding was inhibited by excess unlabeled DNA corresponding to nucleotides -993 to -973 from the HB-EGF promoter, a region that contains a previously recognized composite AP-1/Ets site. (elsevierpure.com)
  • A large superfamily of transcription factors that contain a region rich in BASIC AMINO ACID residues followed by a LEUCINE ZIPPER domain. (lookformedical.com)
  • Moreover, DNA hypomethylation of the COX‑2 promoter region located from ‑1,522 to ‑829 bp might contribute to transcriptional regulation of COX‑2 expression and induce a pro‑inflammatory response in KIC. (spandidos-publications.com)
  • A moderate increase in α5 promoter activity was observed in subconfluent RCECs grown on CIV but not on CI. (nih.gov)
  • Induces up-regulation of FAS promoter activity, and thereby contributes to the increased triglyceride accumulation in hepatocytes (steatosis) (PubMed:14602201). (proteopedia.org)
  • The stretch-mediated increase in promoter activity was completely ablated with deletion of nucleotide positions -1301 to -881. (elsevierpure.com)
  • Stretch-induced promoter activity was significantly inhibited by site-directed mutagenesis of the AP-1 or Ets components of this site. (elsevierpure.com)
  • We sought to examine the potential of JDP2 to inhibit c-Jun/AP-1 oncogenic activity in mice. (biomedcentral.com)
  • The dimer composition of AP-1, the cell type and the stage of development are all important in the regulation of AP-1 activity. (indigobiosciences.com)
  • We conclude that members of the NF-κB family may directly impact regulation of IRF family, while IRF members impact regulation of NF-κB family rather indirectly, via other transcription factors such as AP-1 and SP1. (biomedcentral.com)
  • Represses cell cycle negative regulating factor CDKN1A, thereby interrupting an important check point of normal cell cycle regulation (By similarity). (proteopedia.org)
  • These results demonstrate that the calcium-mediated transient up-regulation of Sema3A in NHEKs is involved in the MEK/ ERK and AP-1 signaling axis. (quantichrome.com)
  • Glucose metabolism is central to the regulation of β-cell function ( 11 ), and GLP-1 is a potent hormonal incretin that has been shown to markedly enhance the stimulatory effects of glucose on β-cells ( 12 , 13 ). (diabetesjournals.org)
  • Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)
  • Primer extension experiments demonstrated the existence of two possible transcription initiation sites at nucleotide -29 and 32 position, 5' to the start of the translation. (elsevierpure.com)
  • transcription initiation[10]. (researchatlanta.org)
  • 2003. Mutational analysis of Ctnnb1 and Apc in tumors from rats given 1,2-dimethylhydrazine or 2-amino-3-methylimidazo[4,5-f]quinoline: mutational 'hotspots' and the relative expression of beta-catenin and c-jun. . (oregonstate.edu)
  • NF-κB also controls expression of the downstream IFN auto-amplification loop through STAT1, IRF-1, −5, and −7 transcription factors. (biomedcentral.com)
  • Calcium-inducible MAPK/AP-1 signaling drives semaphorin 3A expression in normal human epidermal keratinocytes. (quantichrome.com)
  • We previously demonstrated that cyclic mechanical stretch induces expression of the SMC, fibroblast, and epithelial cell mitogen heparin-binding epidermal growth factor-like growth factor (HB-EGF) in bladder SMC, an observation that suggests that this growth factor may be involved in compensatory bladder hypertrophy. (elsevierpure.com)
  • Compared with the control group, the expression of NOX2, nuclear factor-κB (NF-κB), and Snail was increased in vivo and in vitro but decreased with TMZ treatment. (dovepress.com)
  • There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
  • Jakobsson expression under a human GFAP (hGFAP) promoter. (lu.se)
  • Tβ4 levels are transcriptionally regulated via the novel cis -acting element AGACAAAG, which interacts with Smad and T-cell factor/lymphoid enhancer factor (TCF/LEF) to synergistically activate the Tβ4 promoter downstream of TGFβ. (aacrjournals.org)
  • It has been hypothesized that the AP-1 may play a substantial role in the inflammation and immune response (Michel et al. (medscape.com)
  • The NF-κB and IRF transcription factor families are major players in inflammation and antiviral response and act as two major effectors of the innate immune response (IIR). (biomedcentral.com)
  • The variety of AP-1 dimer configurations confer versatility to this transcription factor in regulating numerous physiological (cell proliferation, differentiation, apoptosis, migration) and pathological activities (cancer, inflammation, transplant rejection) in the cell. (indigobiosciences.com)
  • The goal of this study was to investigate the impact of multiple exposomal factors (genetics, lifestyle factors, environmental/occupational exposures) on pulmonary inflammation and corresponding alterations in local/systemic immune parameters. (cdc.gov)
  • Dose-dependent inhibition of AP-1 Human AP-1 Reporter Cells were treated with an ~EC80 concentration of PMA (10 nM) and then challenged with the inhibitors JNK Inhibitor V, CC-401, PD98059, CAY 10561, and PKC inhibitors Bisindolylmaleimide I and XI (all from Cayman Chemical, USA). (indigobiosciences.com)
  • Within this scholarly research the cloning and functional characterization from the individual ITGB6 promoter is described. (researchatlanta.org)
  • The principal application of this reporter assay is in the screening of test compounds to quantify any functional activities, either activating or inhibitory, that they may exert against AP-1. (indigobiosciences.com)
  • Ketamine treatment increased the binding of NF‑κB and permissive histone H3 lysine‑4 (H3K4)m3, but caused a decrease in the repressive histone H3K27m3 and H3K36m3 on the COX‑2 promoter ranging from ‑1,522 to ‑1,331 bp as determined by a chromatin immunoprecipitation assay. (spandidos-publications.com)
  • Prevaccination and 1-month-postvaccination sera were tested by hemagglutination inhibition (HI) assay against 4 cell culture propagated vaccine reference viruses. (cdc.gov)
  • Although the promoters P1 and P3 are used equally in unpolarized macrophages, stimulation with LPS or IFN-γ leads to increased transcription from P3 in inflammatory M1 macrophages. (aai.org)
  • In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)
  • As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. (oncotarget.com)
  • 20(1): 441, 2022 Oct 08. (bvsalud.org)
  • 1 Thus, it is imperative to understand the mechanisms involved in inhibition of myocardial fibrosis. (dovepress.com)
  • We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. (frontiersin.org)
  • This binding initiates a cascade of events that culminates in differentiation into Th1 cells, by increasing the transcription factor T-bet 12 . (bvsalud.org)
  • Using chromatin immunoprecipitation assays this research has confirmed for the very first time that transcription elements STAT3 and C/EBPα get excited about the positive legislation of ITGB6 transcription in dental squamous cell carcinoma cells. (researchatlanta.org)
  • While many factors are involved in the etiology of cancer, it has been clearly established that diet significantly impacts one's risk for this disease. (mdpi.com)
  • Significantly, the actions of both GLP-1 and forskolin were abolished by the selective Ca 2+ /calmodulin-dependent phosphatase 2B (calcineurin) inhibitor, FK506, as well as by the chelation of intracellular Ca 2+ by BAPTA (bis(2-aminophenoxy)ethane- N,N,N ′ ,N ′-tetraacetate). (diabetesjournals.org)
  • HD-IIV3 recipients had similar postvaccination antibody titers compared with SD-IIV4 recipients, whereas RIV4 recipients had significantly higher 1-month-postvaccination antibody titers against vaccine reference viruses for all outcomes. (cdc.gov)
  • It was subsequently shown to regulate several functions unrelated to catabolism, and to be both a negative and a positive regulator of transcription. (bvsalud.org)