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Matrix Attachment Region Binding ProteinsPromoter Regions, GeneticGA-Binding Protein Transcription FactorBase SequenceMolecular Sequence DataDNA-Binding ProteinsTranscription, GeneticBinding SitesGene Expression RegulationTranscription FactorsAmino Acid SequenceCloning, MolecularProtein BindingRNA, MessengerSp1 Transcription FactorCell LineDNATransfectionMutationTranscriptional ActivationGenes, ReporterDNA MethylationPlasmidsGene Expression Regulation, BacterialRegulatory Sequences, Nucleic AcidEscherichia coliProto-Oncogene Proteins c-etsTranscription Initiation SiteElectrophoretic Mobility Shift AssayOperonBacterial ProteinsNuclear ProteinsRepressor ProteinsDNA FootprintingProtein SubunitsDNA PrimersSequence Homology, Nucleic AcidResponse ElementsRestriction MappingChromatin ImmunoprecipitationTrans-ActivatorsLuciferasesChloramphenicol O-AcetyltransferaseConsensus SequenceEnhancer Elements, GeneticHeLa CellsTATA BoxCpG IslandsGenes, Bacterial5' Flanking RegionGABA Plasma Membrane Transport ProteinsRecombinant Fusion ProteinsGene Expression Regulation, EnzymologicMacromolecular SubstancesSequence Analysis, DNASequence DeletionMutagenesis, Site-DirectedExonsDeoxyribonuclease ITumor Cells, CulturedPolymerase Chain ReactionGene ExpressionGenesDNA-Directed RNA PolymerasesCells, CulturedSequence Homology, Amino AcidDNA, BacterialIntronsReverse Transcriptase Polymerase Chain ReactionSequence AlignmentGenes, RegulatorCell Line, TumorCarrier ProteinsRecombinant ProteinsEscherichia coli ProteinsPolymorphism, GeneticGene SilencingCell Nucleusbeta-GalactosidaseOligodeoxyribonucleotidesKineticsHistonesProto-Oncogene Protein c-ets-15' Untranslated RegionsChromatinSignal TransductionChromosome MappingDNA Mutational AnalysisAllelesBlotting, WesternDNA, ComplementaryGenotypeOrganic Anion TransportersOrgan SpecificityConserved SequenceSaccharomyces cerevisiaeMultigene FamilyGene DeletionGene Expression Regulation, NeoplasticProto-Oncogene Proteins
Mediator complex subunitAlpha subunitOverexpressionGABPAProtein transcription factorProteinsEnhancer-promoterGenesGeneTERTMotifsAnkyrin repeatTranscription factorsFunctionalRegulatorSequencesMiceBetaComplexHumanDomainIdentifySiteAnalysisEncodesProteinTranscription factorRetinoic AcidNuclear respiratory factorProteinsResponsive elementAlphaCellRegulationDomains
Mediator complex subunit2
Alpha subunit2
Overexpression3
  • Furthermore overexpression of both GABPβ and GABPα subunits in Cos1 cells led to increased endogenous CyP40 mRNA amounts. (techuniq.com)
  • GA-binding proteins selectively bind at these positions, respectively, causing an activation of the promoter and overexpression of TERT. (oncotarget.com)
  • Overexpression of METTL23 resulted in increased transcriptional activity at the THPO promoter, whereas knockdown of METTL23 with siRNA resulted in decreased expression of ATP5B, thus revealing the importance of METTL23 as a regulator of GABPA function. (bvsalud.org)
GABPA3
  • Promoters of housekeeping genes contain built-in activating motifs for factors such as GABPA and YY1, which decrease the responsiveness of promoters to distal enhancers. (nature.com)
  • GABP is a multimeric protein consisting of GABPA and GABPB with its isoforms GABPB1, GABPB1-L, GABPB1-S, GABPB2. (oncotarget.com)
  • GABP, of which GABPA is a component, is known to regulate the expression of genes such as THPO ( thrombopoietin ) and ATP5B ( ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide ) and is implicated in a wide variety of important cellular functions. (bvsalud.org)
Protein transcription factor1
Proteins7
  • Three tandemly organized Ets sites within this important region were defined as binding components for the multimeric Ets-related transcription aspect GA binding proteins (GABP). (techuniq.com)
  • Launch Cyclophilins and FK506 binding protein (FKBPs) are mobile goals TKI-258 for the immunosuppressive medications cyclosporin A and FK506 respectively and screen a peptidyl- prolyl isomerase (PPIase) function that's thought to catalyze proteins folding (Galat and Metcalfe 1995). (techuniq.com)
  • All GA-binding proteins progress through the glioma grades and have the highest expression levels in secondary glioblastomas. (oncotarget.com)
  • The present study confirms the upregulation of TERT in primary glioblastomas while all GABP proteins rise with the malignancy of the gliomas. (oncotarget.com)
  • Further investigations must be made to elucidate the relation between TERT and all GABP proteins as it may play a key role in the gliomagenesis. (oncotarget.com)
  • ADP ribosylation factors (ARFs), which are members of the Ras superfamily of GTP-binding proteins, are critical components of vesicular trafficking pathways in eukaryotes. (embl.de)
  • Like Ras, ARFs are active in their GTP-bound form, and their duration of activity is controlled by GTPase-activating proteins (GAPs), which assist ARFs in hydrolyzing GTP to GDP. (embl.de)
Enhancer-promoter4
  • Here we design a high-throughput reporter assay called enhancer × promoter self-transcribing active regulatory region sequencing (ExP STARR-seq) and applied it to examine the combinatorial compatibilities of 1,000 enhancer and 1,000 promoter sequences in human K562 cells. (nature.com)
  • We identify simple rules for enhancer-promoter compatibility, whereby most enhancers activate all promoters by similar amounts, and intrinsic enhancer and promoter activities multiplicatively combine to determine RNA output ( R 2 = 0.82). (nature.com)
  • Together, this systematic assessment of enhancer-promoter compatibility suggests a multiplicative model tuned by enhancer and promoter class to control gene transcription in the human genome. (nature.com)
  • van Arensbergen, J., van Steensel, B. & Bussemaker, H. J. In search of the determinants of enhancer-promoter interaction specificity. (nature.com)
Genes5
  • Analyzing 5′-upstream non-protein-encoding regions of the human mitochondrial function-associated genes, we speculate that mitochondrial functions could be recovered or improved at a transcriptional level. (intechopen.com)
  • Promoters of variably expressed genes lack these motifs and show stronger responsiveness to enhancers. (nature.com)
  • Fig. 4: Promoter classes correspond to enhancer responsive versus ubiquitously expressed genes. (nature.com)
  • This project in the first part is simply a promoter and 3' UTR analysis of some 30 genes that appear at the same time in the postsynaptic region of skeletal muscle. (edu.au)
  • Genes having at least one occurrence of the highly conserved motif M53 RYTGCNNRGNAAC in the regions spanning 4 kb centered on their transcription starting sites [-2kb, +2kb]. (gsea-msigdb.org)
Gene7
  • Association of single nucleotide polymorphisms in the nuclear respiratory factor-2 beta subunit-encoding the GABPB1 gene within the occupational environment. (nih.gov)
  • GA-binding protein subunit beta-1 is a protein that in humans is encoded by the GABPB1 gene. (wikipedia.org)
  • GABP is certainly a common focus on of mitogen and stress-activated pathways and could integrate these different extracellular signals to modify CyP40 gene appearance. (techuniq.com)
  • Gene regulation in the human genome is controlled by distal enhancers that activate specific nearby promoters 1 . (nature.com)
  • UC San Francisco researchers have discovered how a mutation in a gene regulator called the TERT promoter -- the third most common mutation among all human cancers and the most common mutation in the deadly brain cancer glioblastoma -- confers "immortality" on tumor cells, enabling the unchecked cell division that powers their aggressive growth. (eurekalert.org)
  • But recent research has suggested that more than 50 types of human cancers may be caused not by a defective TERT gene itself, but by mutations in the TERT promoter -- a region of DNA where protein complexes called transcription factors can influence when and how the TERT gene is activated. (eurekalert.org)
  • Targeted high-throughput sequencing of the exons in the candidate region identified a homozygous 4-bp deletion (c.169_172delCACT) in the METTL23 ( methyltransferase like 23) gene , which is predicted to result in a frameshift and premature truncation (p.His57Valfs*11). (bvsalud.org)
TERT6
  • The research, published September 10, 2018 in Cancer Cell , found that patient-derived glioblastoma cells with TERT promoter mutations depend on a particular form of a protein called GABP for their survival. (eurekalert.org)
  • These findings suggest that the ß1L subunit is a promising new drug target for aggressive glioblastoma and potentially the many other cancers with TERT promoter mutations," said study senior author Joseph Costello, PhD, a leading UCSF neuro-oncology researcher. (eurekalert.org)
  • These mutations enable a transcription factor called GABP to bind to the TERT promoter and activate it, other studies had found, which was strange because in healthy cells GABP and TERT usually have nothing to do with one another. (eurekalert.org)
  • Costello's team, led by graduate students Andrew Mancini and Ana Xavier-Magalhaes, studied human glioblastoma cell lines and primary tumor cells derived from advanced-stage glioblastoma patients and showed that the cells' mutations create two adjacent sequences of DNA in the TERT promoter that make a perfect landing pad for a particular form of the GABP transcription factor complex containing four subunits, one of which was GABP-ß1L. (eurekalert.org)
  • The researchers showed that this GABP-ß1L-containing form of GABP is required to activate TERT and drive cancer growth, but that it appears not to be essential for healthy cells. (eurekalert.org)
  • Enhanced expression of TERT in gliomas is a result of two hotspot mutations, C228T and C250T, at the promoter region. (oncotarget.com)
Motifs2
  • Here, we present a comparative analysis of the human, mouse, rat and dog genomes to create a systematic catalogue of common regulatory motifs in promoters and 3' untranslated regions (3' UTRs). (gsea-msigdb.org)
  • The promoter analysis yields 174 candidate motifs, including most previously known transcription-factor binding sites and 105 new motifs. (gsea-msigdb.org)
Ankyrin repeat1
  • The structure of the IkappaBalpha ankyrin repeat domain, bound to a partially truncated NF-kappaB heterodimer (p50/ p65), has been determined by X-ray crystallography at 2.7 A resolution. (embl.de)
Transcription factors1
Functional1
  • Functional research of the proximal promoter series in conjunction with mutational evaluation confirmed these websites to be essential for basal promoter function. (techuniq.com)
Regulator1
  • Our outcomes recognize GABP as an integral regulator of CyP40 appearance. (techuniq.com)
Sequences1
  • Fig. 5: P2 promoters contain built-in enhancer sequences. (nature.com)
Mice1
  • However, before we can gain a proper understanding of their role in mice that are missing these ion channel subunits, we need to know their developmental expression profile in the developing mouse. (edu.au)
Beta3
  • Each repeat folds into a helix-loop-helix structure with a beta-hairpin/loop region projecting out from the helices at a 90 o angle. (embl.de)
  • integrin subunit beta 8 [Source. (gsea-msigdb.org)
  • ATP synthase F1 subunit beta [So. (gsea-msigdb.org)
Complex1
Human1
  • However, the degree to which human enhancers and promoters are intrinsically compatible has not yet been systematically measured, and how their activities combine to control RNA expression remains unclear. (nature.com)
Domain2
  • PAPbeta, a protein that binds to and is phosphorylated by the non-receptor tyrosine kinase PYK2, contains several modular signaling domains including a pleckstrin homology domain, an SH3 domain, ankyrin repeats and an ARF-GAP domain. (embl.de)
  • WW domain binding protein 1 like. (gsea-msigdb.org)
Identify1
  • You can't create a drug to target a promoter itself, but if we could identify how GABP was binding to the mutated promoter in these cancers, we might have a remarkably powerful new drug target. (eurekalert.org)
Site1
  • This matches the MIF [GeneSymbol=MIF] transcription factor binding site V$MIF1_01 (v7.4 TRANSFAC). (gsea-msigdb.org)
Analysis1
  • Homozygosity mapping and linkage analysis identified a candidate region on chromosome 17 with a maximum multipoint logarithm of odds score of 6.01. (bvsalud.org)
Encodes3
  • This gene encodes the GA-binding protein transcription factor, beta subunit. (wikipedia.org)
  • SDHD encodes subunit D of the succinate dehydrogenase complex, an integral membrane protein. (nih.gov)
  • This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. (cancerindex.org)
Protein12
  • GA-binding protein subunit beta-1 is a protein that in humans is encoded by the GABPB1 gene. (wikipedia.org)
  • This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. (wikipedia.org)
  • 7. Regulation of the human protein S gene promoter by liver enriched transcription factors. (nih.gov)
  • 15. Identification of a responsible promoter region and a key transcription factor, CCAAT/enhancer-binding protein epsilon, for up-regulation of PHGPx in HL60 cells stimulated with TNF alpha. (nih.gov)
  • PAPbeta, a protein that binds to and is phosphorylated by the non-receptor tyrosine kinase PYK2, contains several modular signaling domains including a pleckstrin homology domain, an SH3 domain, ankyrin repeats and an ARF-GAP domain. (embl.de)
  • The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. (lookformedical.com)
  • boule homolog, RNA binding protein [S. (gsea-msigdb.org)
  • damage specific DNA binding protein 2. (gsea-msigdb.org)
  • FYN binding protein 1 [Source:HGNC Sy. (gsea-msigdb.org)
  • G protein subunit alpha q [Source:HGN. (gsea-msigdb.org)
  • RB binding protein 4, chromatin remod. (gsea-msigdb.org)
  • protein_coding" "Cz04g30080.t1","No alias","Chromochloris zofingiensis","Mitochondrial import inner membrane translocase subunit Tim21 [Interproscan]. (ntu.edu.sg)
Transcription factor4
  • 14. Transcriptional activity and Sp 1/3 transcription factor binding to the P1 promoter sequences of the human AbetaH-J-J locus. (nih.gov)
  • 16. Transcription factor GABP/NRF-2 controlling biogenesis of mitochondria regulates basal expression of peroxiredoxin V but the mitochondrial function of peroxiredoxin V is dispensable in the dog. (nih.gov)
  • The motif does not match any known transcription factor binding site. (gsea-msigdb.org)
  • The promoter analysis yields 174 candidate motifs, including most previously known transcription-factor binding sites and 105 new motifs. (gsea-msigdb.org)
Retinoic Acid1
Nuclear respiratory factor1
  • 3. Is nuclear respiratory factor 2 a master transcriptional coordinator for all ten nuclear-encoded cytochrome c oxidase subunits in neurons? (nih.gov)
Proteins3
  • ADP ribosylation factors (ARFs), which are members of the Ras superfamily of GTP-binding proteins, are critical components of vesicular trafficking pathways in eukaryotes. (embl.de)
  • Like Ras, ARFs are active in their GTP-bound form, and their duration of activity is controlled by GTPase-activating proteins (GAPs), which assist ARFs in hydrolyzing GTP to GDP. (embl.de)
  • Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. (lookformedical.com)
Responsive element2
Alpha1
Cell1
  • 19. A three-kilobase fragment of the human Robo4 promoter directs cell type-specific expression in endothelium. (nih.gov)
Regulation1
  • 11. Transcriptional regulation of the human PNRC promoter by NFY in HepG2 cells. (nih.gov)
Domains1
  • It shows a stack of six IkappaBalpha ankyrin repeats facing the C-terminal domains of the NF-kappaB Rel homology regions. (embl.de)