Promoter ccaat enhancer binding proteins. Medical search. Frequent questions

They promote the expression of certain genes through interaction with their promoters. (wikipedia.org)
A.D. Friedman, W.H. Landschulz, S.L. McKnight, "CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells," Genes & Development, 3: 1314-22, 1989. (the-scientist.com)
Gene transcription is mainly regulated by transcription factors (TFs) that bind to specific DNA sequences (called motifs) located in the promoter regions of genes [ 1 ]. (biomedcentral.com)
We mapped all estrogen receptor and RNA polymerase II binding sites on a genome-wide scale, identifying the authentic cis binding sites and target genes, in breast cancer cells. (nature.com)
The C/EBP-α (CCAAT/enhancer-binding protein, alpha), is a family of transcription factor and recognizes the CGCAAT motif in the promoters of target genes. (gentarget.com)
Here, we identify the molecular chaperones heat shock proteins (Hsp) 90α and β as target genes of both pathways. (ashpublications.org)
We found that a sequestration of C/EBPα into complexes with Brm leads to a weak interaction of C/EBPα with promoters of liver-specific genes, expression of which is reduced in old animals. (utmb.edu)
Injection of either GH or the regulator of the amplitude of endogenous GH release, ghrelin, reduces the C/EBPα-Brm complex in livers of old mice, leading to a derepression of E2F targets, to increased interactions of C/EBPα with promoters of liver-specific genes, and to correction of their expression. (utmb.edu)
Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. (affbiotech.com)
Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. (affbiotech.com)

TGZ elevated DR5 expression at the promoter level through the CCAAT/enhancer-binding protein homologous protein (CHOP) binding site. (nih.gov)
We demonstrate for the first time that resveratrol induce CCAAT/enhancer-binding protein-homologous protein (CHOP). (dsmc.or.kr)
CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). (affbiotech.com)

CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells. (johnshopkins.edu)
Dive into the research topics of 'CCAAT/enhancer binding protein activates the promoter of the serum albumin gene in cultured hepatoma cells. (johnshopkins.edu)
Nuclear FoxO3a activates the atrogin1 promoter ( 27 ). (spandidos-publications.com)

These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation. (harvard.edu)
Results DIM, but not I3C, increased adipocyte differentiation through upregulation of peroxisome proliferator‐activated receptor γ and CCAAT/enhancer‐binding protein α. (researchgate.net)
Taken together, radiation-induced histone alterations at the CXCL12 promoter in hepatoma cells are linked to CXCL12 upregulation and increased aggressiveness in the tumor microenvironment. (molcells.org)

Because MMP-13 and its regulatory networks are suitable targets for the development of effective early treatment strategies for OA, we discuss the specific targets of MMP-13, including upstream regulatory proteins, DNA methylation, non-coding RNAs, and autophagy-related proteins of MMP-13, and their therapeutic potential to inhibit the development of OA. (biomedcentral.com)
An operating TATA container was determined in ITGB6 promoter upstream from the transcription-initiation site (TSS). (researchatlanta.org)
Using PCR generated, site-specific probes corresponding to the non-coding exons 1 and 2 and intron 2 of CHOP, rearrangements in five of seven tumors mapped to the 2.4 and 1.6 kbp PstI fragments that contain the first two exons and introns of the gene and the upstream promoter region. (lu.se)

JASPAR and TFSEARCH were used to analyze the potential transcription factor binding sites in the promoter region of chicken VNN1 and miRanda was used to search miRNA binding sites in 3' untranslated region (3'UTR) of chicken VNN1. (koreascience.kr)

CCAAT/enhancer-binding protein β (C/EBPβ), is a member of a family of transcription factors consisting of six structurally related basic leucine-zipper DNA-binding proteins. (biomedcentral.com)

The proximal region of the cNANOG promoter contains a positive transcriptional regulatory element (CCAAT/enhancer-binding protein ( CEBP )-binding site) in ESCs. (biomedcentral.com)
Direct binding of ZFP143 to the proximal region of the NANOG promoter regulates NANOG expression by modulating OCT4 binding [ 24 ]. (biomedcentral.com)

An expression vector capable of encoding full-length CCAAT/enhancer-binding protein (C/EBP) has been constructed and tested in transient transfection assays for its capacity to activate transcription from the promoter of the serum albumin gene. (johnshopkins.edu)
When tested in cultured hepatoma cells, the C/EBP expression vector achieved potent trans-activation of the albumin promoter. (johnshopkins.edu)
Expression vectors that encoded defective forms of C/EBP failed to activate the albumin promoter. (johnshopkins.edu)
The CCR5 promoter polymorphisms were significantly associated with cervical intraepithelial neoplasia by altering the expression of CCR5 on the cell surface in a Chinese Han population. (biomedcentral.com)
Variability of the promoter region of CCR5 gene might be the reason for differing CCR5 expression levels. (biomedcentral.com)
An increase in CCL3 expression and promoter activity was observed in NP cells after TNF α or IL-1_ treatment. (shu.ac.uk)
We have previously reported that C/EBP beta specifically binds to the CRE on the proximal Pgc-1 alpha promoter and increases forskolin-sensitive Pgc-1 alpha and Ucp1 expression in white 3T3-L1 preadipocytes. (elsevierpure.com)
We conclude that the increased cAMP stimulation of Pgc-1 alpha expression is regulated by the combinatorial effect of transcription factors acting at the CRE on the proximal Pgc-1 alpha promoter. (elsevierpure.com)
The expression of Lcn2 is induced during 3T3-L1 adipogenesis in a CCAAT/enhancer-binding protein-dependent manner. (diabetesjournals.org)
Resveratrol-induced CHOP mRNA (and also protein) expression was inhibited by JNK specific inhibitor, but not ERK, p38 MAPK, PI3K and NF-κB inhibitors. (dsmc.or.kr)
Resveratrol-induced expression of CHOP involves the putative Sp1 site within the CHOP promoter region. (dsmc.or.kr)
It also allows to generate your own reporting cell line in your desired cell type for study or screen of pathway specific gene-knockdown, over-expression, or chemical / drug/protein treatment in the cell based assay. (gentarget.com)
We also found that MC and PN were responsible for the anti-adipogenetic effect of KSS and significantly suppressed the expression of CCAAT/enhancer-binding proteins-δ (C/EBP-δ) mRNA 3 days after the induction of differentiation. (elsevierpure.com)
Taken together, these results suggest that PN suppresses C/EBP-δ expression by inhibiting Dex-induced GR promoter activity at the early stage of differentiation and, consequently, delays differentiation into mature adipocytes. (elsevierpure.com)
Additionally, CCAAT enhancer binding protein (C/EBP)β levels are elevated in brain specimens from HIV-1 patients and the transcription factor contributes to astrocyte TIMP-1 expression. (utmb.edu)
TIMP-1 and C/EBPβ mRNA and protein expression were evaluated at 12 and 24 h post-treatment, respectively. (utmb.edu)
Compared with rabbits breathing room air, hypoxia in rabbits reduced the amount of CYP1A1, 1A2, 2B4, 2C5, and 2C16 proteins and increased the expression of CYP3A6. (aspetjournals.org)
In vivo, in rats, hypoxia increased the mRNA and protein expression of P-glycoprotein but did not affect the mRNA of breast cancer resistance protein and organic anion-transporting polypeptide 2. (aspetjournals.org)
In vivo, the increase in CYP3A6 by hypoxia is associated with the increased expression and nuclear translocation of hypoxia-inducible factor-1 (HIF-1), activator protein 1 (AP-1), and constitutive androstane receptor ( Fradette and du Souich, 2003 ). (aspetjournals.org)
CCAAT/enhancer-binding protein beta (Cebpb) is a key factor of Runx2 expression and our previous study has reported two CCD signs including hyperdontia and elongated coronoid process of the mandible in Cebpb deficient mice. (biomedcentral.com)

Figure 4: Identification of enriched motifs within the estrogen receptor binding sites and validation of transcription factor binding. (nature.com)

H3K4 methylation was also enriched and H3K9 methylation was decreased at its promoter region. (molcells.org)

The discovery of leptin more than a decade ago established a paradigm in which secreted proteins from adipocytes coordinate energy balance and glucose homeostasis ( 2 , 3 ). (diabetesjournals.org)

C/EBPβ can interact with CREB, NF-κB, and other proteins, leading to a trans-activation potential. (wikipedia.org)
In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. (harvard.edu)
CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. (harvard.edu)
Employing chromatin immunoprecipitation, we demonstrate that C/EBP beta, CREB, ATF-2, and CHOP10 are bound to the Pgc-1 alpha proximal CRE, but CHOP10 does not bind in HIB-1B cell lysates. (elsevierpure.com)
Forskolin stimulation and C/EBP beta overexpression in 3T3-L1 cells increased C/EBP beta and CREB but displaced ATF-2 and CHOP10 binding to the Pgc-1 alpha proximal CRE. (elsevierpure.com)

RCAN binding to calcineurin, a Ca 2+ /calmodulin-dependent phosphatase, inhibits calcineurin activity, thereby regulating different physiological events via dephosphorylation of important substrates. (molcells.org)
The reduced proliferative capacities of the liver of old animals are associated with the CCAAT/enhancer-binding protein α (C/EBPα)-Brm complex, which inhibits E2F-dependent promoters. (utmb.edu)

Taken together, the present studies suggest that induction of CHOP protein may be involved, at least in part, in resveratrol-induced apoptosis. (dsmc.or.kr)

RCAN1 is upregulated by stress factors, such as protein aggregates, elevated intracellular calcium, oxidative stress, and glucocorticoid, prevents excess and dangerous over-activation of calcineurin. (molcells.org)
In addition, PN inhibited dexamethasone (Dex)-induced glucocorticoid receptor (GR) promoter activity. (elsevierpure.com)

Binding of complement to a foreign substance, or antigen, amplifies and augments the body's innate immune system by means of its role as an opsonin (a factor that enhances phagocytosis of unwanted particles) and as a chemoattractant (a factor that recruits cells to areas of inflammation). (medscape.com)
After a complex process of education and maturation, a circulating lymphocyte can bind to an antigen. (medscape.com)
To demonstrate the flexibility and modular nature of the liposome system, 10 recombinant surface antigens representing distinct influenza virus strains were bound simultaneously to generate a highly multivalent protein particle that with 5 ng individual antigen dosing induced antibodies in mice that specifically recognized the constituent immunogens and conferred protection against heterologous H5N1 influenza virus challenge. (cdc.gov)
The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a candidate vaccine antigen that binds angiotensin-converting enzyme 2(ACE2), leading to virus entry. (cdc.gov)
Antigen binding via His-tag insertion into the CoPoP bilayer results in a serum-stable and conformationally intact display of the RBD on the liposome surface. (cdc.gov)

Then, we measured the promoter activity of various 5′ flanking regions of cNANOG in chicken PGCs and ESCs using the luciferase reporter assay. (biomedcentral.com)

This family of proteins comprises two subfamilies that share a DNA-binding and dimerization domain called the Rel homology domain (RHD) [ 4 ] and form homo- or hetero- dimers. (oncotarget.com)

Transfections were used to determine the role of NF-κB,CCAAT/enhancer binding protein (C/EBPβ), and MAPK on cytokine-mediated CCL3 promoter activity. (shu.ac.uk)

Therefore, identification of regulatory elements within the promoter region is considered crucial to understand the mechanism underlying transcriptional regulation in specific cell types. (biomedcentral.com)

The first subfamily of proteins (c-Rel, RelB, p65/RelA) contains a C-terminal transactivation domain. (oncotarget.com)
The second subfamily of proteins (p105 and p100) has a C-terminal region that contains multiple copies of ankyrin repeats, instead of a transactivation domain, and can bind to and inhibit Rel proteins. (oncotarget.com)

IκBζ, an atypical member of the nuclear IκB family of proteins, is expressed at low levels in most resting cells, but is induced upon stimulation of Toll-like/IL-1 receptors through an IRAK1/IRAK4/NFκB-dependent pathway. (oncotarget.com)
Nuclear extracts and cytoplasmic protein from RA-FLS treated with cytokines were analyzed. (biomedcentral.com)

Alan D. Friedman (Johns Hopkins Oncology Center, Baltimore): "CCAAT/enhancer binding protein (C/EBP) is perhaps best known as the prototypical basic region-leucine zipper DNA-binding protein (Science, 243:1681-88, 1989). (the-scientist.com)
The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced differentiation of bone marrow cells. (harvard.edu)
In the version of this article initially published online, the estrogen receptor and RNA PolII binding data were aligned incorrectly in the top panel of Figure 3, which represents estrogen receptor binding at the ESR1 gene region. (nature.com)
PkDBPα region II (PkDBPαII) from Peninsular Malaysia and Malaysian Borneo has been shown to occur as distinct haplotypes, and the predominant haplotypes from these geographical areas demonstrated differences in binding activity to human erythrocytes in erythrocyte binding assays. (afpm.org.my)

[ 1 ] Neutrophils move to the site of invasion by means of chemotaxis, which occurs in response to microbial products, activated complement proteins, and cytokines. (medscape.com)

C/EBP proteins are involved in different cellular responses, such as in the control of cellular proliferation, growth and differentiation, in metabolism, and in immunity. (wikipedia.org)
Brain and muscle Arnt-like protein-1 ( BMAL1 ) knockout mice exhibit accelerated aging, abnormal glucose metabolism, and impaired adipocyte differentiation, among other phenotypes, which are effects associated with the BMAL1 gene. (go.jp)
We have used Southern blot technique to test whether a gene of the CCAAT/enhancer binding protein (C/EBP) family, CHOP, which maps to 12q13 and is assumed to be involved in adipocyte differentiation, could be the 12q gene in question. (lu.se)

In contrast, c-Rel and RelB had little effect on promoter activity. (shu.ac.uk)

C/EBP proteins also contain activation domains at the N-terminus and regulatory domains. (wikipedia.org)
Many studies have investigated germ cell-specific gene promoters to understand their regulatory mechanisms. (biomedcentral.com)
All six members of this family exhibit a high sequence similarity in their C-terminal DNA binding domain and a more diverge regulatory N-terminus [ 1 , 2 ]. (biomedcentral.com)

Myostatin binding to type IIB activin receptor (ActRIIB) on muscle surface induces the recruitment and activation of activin receptor-like kinase 5 (ALK5), and eventually leads to forkhead box O3 (FoxO3a)-dependent transcription to promote muscle protein breakdown via the ubiquitin-proteasome system ( 23 ). (spandidos-publications.com)

C/EBPε has two, which lead to four isoforms due to an alternative use of promoters and splicing. (wikipedia.org)

p100 and p105 can however undergo limited proteolysis to generate p52 and p50, respectively, which can form heterodimers with Rel proteins to form transcriptional activators [ 5 ]. (oncotarget.com)
Binds DNA as a dimer and can form stable heterodimers with C/EBP delta. (lu.se)

Using a combination of the Sp1 cDNA transfection, the luciferase reporter assay and Sp1 inhibitor assay, we found that Sp1 site is required for resveratrol-mediated activation of the CHOP promoter. (dsmc.or.kr)

Once bound to DNA, C/EBPs can recruit so-called co-activators (such as CBP) that in turn can open up chromatin structure or recruit basal transcription factors. (wikipedia.org)

TIMP-1 promoter-driven luciferase plasmids were used to evaluate TIMP-1 promoter activity in inhibitor-treated astrocytes. (utmb.edu)

Opsonins are often antibodies or components of the complement pathway that bind to the surface of target organisms to facilitate this phagocytosis. (medscape.com)
The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma following SARS-CoV-2 infection are unknown. (cdc.gov)
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. (affbiotech.com)

The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4). (affbiotech.com)

Long noncoding RNAs (lncRNAs) are a class of endogenous, non-protein coding RNAs that are highly linked to various cellular functions and pathological process. (biomedcentral.com)

Figure 5: Involvement of cooperating transcription factors at estrogen receptor binding sites. (nature.com)

GH-dependent elimination of the complex is mediated by the inhibition of cyclin D3-CDK4 activity and by elevation of a phosphatase, protein phosphatase 2A, which dephosphorylates C/EBPα and dissociates the complex. (utmb.edu)
The ERK1/2-selective inhibitor abrogated IL-1β-mediated increases in TIMP-1 promoter activity. (utmb.edu)

Those reporting lentivirus also contains a constitutively expressed fluorescent selection marker or an antibiotic selection marker under the RSV promoter, which makes it easier to select the stably infected signal reporting cells (to generate pathway specific sensor cell lines), and also provides internal reference for virus transduction efficiency when a fluorescent marker is under the RSV promoter. (gentarget.com)

In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. (harvard.edu)

(B, C) Western blotting for C/EBPβ protein consisting of 38 kD liver-enriched activator protein (LAP*), 36 kD LAP and 20 kD liver-enriched inhibitory protein (LIP) (B) Experiments with whole extracts of primary cultured passage-1 RA fibroblast-like synoviocytes (RA-FLS) from three patients (RA1, RA2 and RA3) and normal human fibroblast-like synoviocytes (HFLS). (biomedcentral.com)

Avian species do not have brown adipose tissue (BAT), and lack Uncoupling Protein 1 ( UCP1 ) [ 12 ]. (biomedcentral.com)

Moreover, mutated variants of the albumin promoter that lack the C/EBP-binding site failed to be trans-activated. (johnshopkins.edu)
Analysis of the mouse C3 promoter showed that it is directly regulated by C/EBPβ through a C/EBPβ consensus site located at position −616/-599 of the gene. (biomedcentral.com)

This protein is expressed in the mammalian nervous system and plays a significant role in the development and function of nerve cells. (wikipedia.org)
Various cell types can process and present these antigens to T cells, or antigens may be soluble and bound to B-cell receptors. (medscape.com)
The oncogenic CXCL12 mRNA and protein were more highly expressed in residual cancer cells from a hepatoma heterotopic murine tumor microenvironment and coculture of human hepatoma Huh7 and normal IMR90 cells after radiation. (molcells.org)

The dimerization is necessary to enable C/EBPs to bind specifically to DNA through a palindromic sequence in the major groove of the DNA. (wikipedia.org)
When C/EBP binds to C/EBP-TRE sequence, the downstream reporter is expressed as the result of activation for the minimal promoter. (gentarget.com)

Anatomy32

Organisms11

Diseases6

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment26

Phenomena and Processes79

Disciplines and Occupations1

Information Science3

Genes10

Homologous protein3

Activates3

Upregulation3

Upstream3

Sites in the promoter1

Basic-leucine zipper1

Proximal region2

Expression20

Motifs1

Methylation1

Adipocytes1

CREB5

Inhibits2

Apoptosis1

Glucocorticoid2

Antigen5

Regions1

Homology1

MAPK1

Underlying transcriptional1

Subfamily2

Nuclear2

Region4

Cytokines1

Differentiation3

RelB1

Regulatory3

Forkhead box1

Isoforms1

Heterodimers2

CDNA1

Chromatin1

Luciferase1

Antibodies3

Oxidative stress1

RNAs1

Involvement1

Activity2

Pathway1

Contrast1

Activator1

Ucp11

Site2

Cells3

Sequence2