• In this study, it was observed that the ion implantation stimulated the neural proliferation and the implantation of different ions on cell culture surfaces was essential to determine the effects of this technique on adhesion, proliferation, differentiation and apoptosis properties of cells in details. (omicsonline.org)
  • The differentiation, proliferation, prognosis, and safety of MSCs for in vivo therapy remain controversial. (molvis.org)
  • In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
  • Symmetric cell division prompts multiplication and asymmetric cell division is an enlightening advance for differentiation. (researchsquare.com)
  • We were able to quantify cellular proliferation and differentiation rates of many cell types across the entire brain in a single experiment, which wasn't possible using conventional approaches," Cao says. (scitechdaily.com)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Thus, neurotrophic signaling may trigger apoptosis rather than survival pathways in cells expressing the p75 receptor in the absence of Trk receptors. (wikipedia.org)
  • While some assays utilize antibodies to study cell health, proliferation, cell cycle or apoptosis, other types of experiments can rely on non-antibody based methods of assessment, often called non-antibody chemical probes. (biolegend.com)
  • Cellular division for any cell type is dependent on the inherent function, location and the response of cells to repair, apoptosis, or death. (biolegend.com)
  • Learn more about our wide selection of assays for cell proliferation, cell viability, and apoptosis . (biotium.com)
  • Wnt-5a has since been suggested as a candidate ligand for ROR1, and ROR1 has been implicated to function as a pseudokinase, promoting proliferation and resistance to apoptosis in cancer cells through interaction with Wnt-5a, and TCL1-co-activation of AKT. (peprotech.com)
  • Flow cytometry is a technique for counting and examining microscopic particles, such as cells and beads, by suspending them in a stream of fluid and passing them by a detection apparatus. (nih.gov)
  • The labeled cells can be visualized using fluorescent imaging, quantified using flow cytometry, and isolated using fluorescence-activated cell sorting (FACS).The ALDEFLUOR™ fluorescent reagent system offers a novel approach to the non-immunological identification, evaluation and isolation of neural stem and progenitor cells. (stemcell.com)
  • 5(6)-Carboxyfluorescein diacetate N -succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. (sigmaaldrich.com)
  • Investigating FlowSight® imaging flow cytometry as a platform to assess chemically induced micronuclei using human lymphoblastoid cells in vitro. (swansea.ac.uk)
  • Kits to covalently label dead cells, allowing cells with permeable plasma membranes to be excluded from analysis in flow cytometry. (biotium.com)
  • Fixable cytoplasmic stains for monitoring cell division by flow cytometry. (biotium.com)
  • Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)
  • Oligodendrocytes, the myelinating cells of the central nervous system (CNS), are generated from oligodendrocyte precursor cells (OPCs) that express neurotransmitter receptors. (nature.com)
  • Conditional knockout of α1A adrenergic receptors in OPCs suppresses spontaneous and locomotion-induced Ca 2+ increases and reduces OPC proliferation. (nature.com)
  • BDNF binds at least two receptors on the surface of cells that are capable of responding to this growth factor, TrkB (pronounced "Track B") and the LNGFR (for low-affinity nerve growth factor receptor, also known as p75). (wikipedia.org)
  • First identified during PCR-based cloning of a human neuroblastoma cell line in search of tyrosine kinases similar to tropomyosin-receptor-kinase (Trk) neurotropic receptors, ROR1, along with the related receptor tyrosine kinase (RTK) ROR2, was catalogued as an "orphan" receptor due to the fact its related ligand remained elusive. (peprotech.com)
  • Sokullu E, Dağcı T, Gözen O, Ersoy F, Öztarhan A (2017) The Effects of Carbon and Gold Ion Implanted Surfaces on Neuronal Stem Cells' Functions. (omicsonline.org)
  • In November 2017, Bayer and Loxo Oncology entered into a global collaboration for the joint development and commercialization of the TRK inhibitors larotrectinib and BAY 2731954 (LOXO-195). (worldpharmanews.com)
  • In July 2017, a multi-center Phase I/II trial in patients with TRK fusion cancers who have progressed while receiving another TRK inhibitor or are intolerant to another TRK inhibitor was initiated. (worldpharmanews.com)
  • Whether you're starting from primary cells, tissue samples, or pluripotent stem cells, explore our complete portfolio of products to support your neural culture workflow. (stemcell.com)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • These strat- egies provide a robust way to visualize and isolate specific cell pop- ulations of differentiated pluripotent stem cells. (lu.se)
  • NTRK gene fusions are genomic alterations that result in constitutively-activated chimeric TRK fusion proteins, which act as an oncogenic driver, promoting cell proliferation and survival in tumor cell lines. (worldpharmanews.com)
  • The development of targeted therapies in pancreatobiliary cancers has been hampered by the difficulty in obtaining biopsies with sufficient tumor cell content for genomic sequencing analyses. (aacrjournals.org)
  • The mechanisms that control tumor cell dormancy remain poorly understood, making it difficult to predict which patients will recur and thus benefit from more rigorous screening and treatments. (biomedcentral.com)
  • ATP-Glo ™ Bioluminometric Cell Viability luciferase assay. (biotium.com)
  • ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. (biotium.com)
  • To study PDX cells preclinically, we used both bioluminescence imaging (BLI) to evaluate cell viability and magnetic particle imaging (MPI), an emerging imaging technology to allow for detection and quantification of iron nanoparticles. (biorxiv.org)
  • Data on cell proliferation were obtained by labelling replicating cells, and by careful assessment of cell viability for each experimental sample. (johnshopkins.edu)
  • In the currently available test systems for migration inhibition of NCC (MINC), the manual generation of a cell-free space results in extreme operator dependencies, and limits throughput. (johnshopkins.edu)
  • Moreover, inflammatory cell infiltration and fibrosis were found in the control group with only SIS, but not in the experimental autologous USC-seeded SIS group. (biomedcentral.com)
  • These porous microstructures allow cell infiltration into the matrix and have fewer heterogeneous cellular proteins, thereby preventing complications, such as inflammatory reactions, fibrosis, calcification, and graft shrinkage. (biomedcentral.com)
  • OBJECTIVE: Human adipose-derived mesenchymal stem cell exosomes (ADSC-Exos) are active constituents for treating liver fibrosis. (bvsalud.org)
  • METHODS: The cell models of hepatic fibrosis were established by inducing LX-2 cells with TGF-ß1. (bvsalud.org)
  • AiViva has developed innovative approaches to address high unmet medical needs through focal therapies that target diseases of neovascularization, abnormal cell proliferation, and fibrosis. (tmcnet.com)
  • NT3 upregulates cellular proliferation, extracellular matrix protein production, and collagen deposition in human aortic valve interstitial cells through the Trk-Akt-cyclin D1 cascade. (cusabio.com)
  • TRK fusions can be found in many types of solid tumors and affect both children and adults. (worldpharmanews.com)
  • Fusions involving the tropomyosin-related kinase (TRK) family of neurotrophin receptor tyrosine kinases have been identified in various human cancers and suggested to function as oncogenic drivers. (aacrjournals.org)
  • Preclinical studies demonstrated that LOXO-101 inhibited the proliferation of cancer cell lines harboring oncogenic TRK fusions in a dose-dependent manner and resulted in suppression of tumor growth in vivo , consistent with previous studies. (aacrjournals.org)
  • In biological radiation research, cellular responses after irradiation are usually studied in vitro, i.e. on cells in so-called cell culture. (unibw.de)
  • To solve these problems, we are developing a new method here at the institute: We microscope the cells after irradiation with phase contrast and analyze the resulting videos with the algorithm CeCILE (Cell Classification and In-vitro Lifecycle Evaluation), which is based on artificial intelligence. (unibw.de)
  • HADSCs were induced to confirm that they can express the characteristics of endothelial cells (ECs) in vitro. (molvis.org)
  • Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
  • Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)
  • article{ff9b980d-2e89-453c-b22b-428d2e903c6f, abstract = {{Although long-term repopulating hematopoietic stem cells (HSC) can self-renew and expand extensively in vivo, most efforts at expanding HSC in vitro have proved unsuccessful and have frequently resulted in compromised rather than improved HSC grafts. (lu.se)
  • The TRK inhibitor LOXO-101 inhibits the growth of TRK fusion-positive cells in vitro and in vivo . (aacrjournals.org)
  • Compared with other closely related isolates, representative isolates of the RdJ clone more effectively evaded immune function related to monocytic cells, as evidenced by decreased phagocytosis rate and increased numbers of viable unphagocytosed (free) bacteria after in vitro exposure to monocytes. (cdc.gov)
  • Sensitization of cancer cells to conventional drugs using multi-target agents that block survival and oncogenic pathways, alone or in combination, is an emerging strategy to overcome drug resistance. (researchsquare.com)
  • Rapamycin works by inhibiting mTOR, which is involved in cell survival and proliferation. (stjude.org)
  • They have complementary mechanisms to hinder the carcinogenic process by searching free radicals, inhibiting survival and multiplication of malignant cells, as well as reducing invasiveness and angiogenesis of tumors. (researchsquare.com)
  • Receptor-tyrosine-kinase-like orphan receptor 1 (ROR1) is a tumor-associated, surface protein predominantly expressed during embryogenesis, where it is involved in organ morphogenesis, nervous system development, and neural progenitor cell maintenance and survival. (peprotech.com)
  • Virtually absent from normal pediatric and adult tissues, with the exception of low-level expression in a subset of immature b cell precursors known as hematogones and adipocytes, ROR1 is notably overexpressed, and considered a survival factor, in a number of B lymphoid and epithelial malignancies: including chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), marginal zone lymphoma, lung adenocarcinoma. (peprotech.com)
  • Additionally, the incidence of brain metastasis (BM) is increasing, as neuroimaging techniques improve and treatments that allow for longer patient survival permits more time for cells to metastasize to the central nervous system ( 6 , 7 ). (biorxiv.org)
  • Genes enriched in dormant breast cancer cells correlate with recurrence-free survival in breast cancer patients. (biomedcentral.com)
  • Overall survival and QoL will not be tracked. (medscape.com)
  • The study concluded that low tumor burden and a low proliferation index represent independent prognostic factors for long progression-free survival, while previous transcatheter arterial chemoembolization (TACE) techniques represent independent prognostic factors for early tumor progression and shorter progression-free survival. (medscape.com)
  • Unlike antibodies, the T cells recognize flu viruses based on shared internal proteins. (stjude.org)
  • Larotrectinib is a CNS active TRK inhibitor designed to inhibit these proteins. (worldpharmanews.com)
  • By identifying in spinal fluid how the characteristic mutant proteins of Huntington's disease spread from cell to cell, researchers have created a new method to quickly and accurately track the presence and proliferation of these neuron-damaging compounds - a discovery that may accelerate the development of new drugs to treat this incurable disease. (neurodegenerationresearch.eu)
  • The researchers added that the cell-to-cell „seeding" property of these mutant proteins seems to be a critical part of the disease's progression. (neurodegenerationresearch.eu)
  • Although the vast majority of neurons in the mammalian brain are formed prenatally, parts of the adult brain retain the ability to grow new neurons from neural stem cells in a process known as neurogenesis. (wikipedia.org)
  • Primary antibodies are available for the characterization of neural stem cells, neural progenitor cells and their progeny. (stemcell.com)
  • Using this surface modification method, it was aimed to improve surface characteristics and achieve a bioactive surface for neural stem cells. (omicsonline.org)
  • Even though the integration of stem cells was promising, neural stem cell studies still have many milestones to reach. (omicsonline.org)
  • We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
  • They develop from progenitor cells-descendants of adult stem cells that differentiate into specialized cell types. (scitechdaily.com)
  • Here, we demonstrated the feasibility of using autologous urine-derived stem cells (USCs) seeded on small intestinal submucosa (SIS) to repair a urethral defect in a rabbit model. (biomedcentral.com)
  • The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • With the first-ever approved TRK inhibitor, larotrectinib, and BAY 2731954 progressing through clinical development, we have two very promising compounds in our precision oncology portfolio and we are committed to expanding this portfolio by bringing forward highly differentiated and promising additional projects. (worldpharmanews.com)
  • The orally available, highly selective small-molecule TRK family inhibitor LOXO-101 is currently being tested in a phase I dose-escalation study in patients with advanced solid tumors. (aacrjournals.org)
  • This enhancement enables the meticulous labeling and tracking of the dynamics of rare progenitor cells in mammalian organs. (scitechdaily.com)
  • For the current study, the researchers analyzed more than 10,000 newborn progenitor cells from across entire mouse brains spanning three ages (young, mature, and elderly) with a synthetic molecule known as 5-ethynyl-2-deoxyuridine (EdU). (scitechdaily.com)
  • Downloaded from periodic thymic colonization by progenitor cells guarantees stage. (lu.se)
  • Neuroscientists have long sought better tools to study different cells in the brain, including neurons (which transmit electrical and chemical messages) and microglia (immune cells responsible for maintaining brain health). (neurosciencenews.com)
  • Then, targeting single neurons for analysis, they used the devices to record the electrical activity of those same cells over the course of the mice's adult lives. (neurosciencenews.com)
  • Even after one year, we didn't see any degradation of the individual neurons or proliferation of the microglia we were interested in recording with the devices," Liu says. (neurosciencenews.com)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
  • His lab specializes in optimizing methods for single-cell sequencing, an increasingly popular approach to analysis that homes in on the genetic expression and molecular dynamics of individual cells. (scitechdaily.com)
  • This innovative technique allowed the researchers to analyze tens of thousands of gene expressions and the chromatin landscapes of these newborn cells as they grew into families of cell types with different molecular functions. (scitechdaily.com)
  • Those only capture static information-the current molecular state of a cell at a single moment. (scitechdaily.com)
  • The breakthrough format of nanotechnology offers innovative solutions, giving researchers greater analytical capacity, improved data quality and at the same time consuming less sample volume in the storage and screening of molecular, cell and tissue libraries. (ddw-online.com)
  • Correspondence with low molecular weight chemicals that results in the priming of chemical-specific, Prof. Dr. Stefan F. Martin, Allergy Research skin-homing CD8+ Tc1/Tc17 and CD4+ Th1/Th17 cells. (cdc.gov)
  • This radiolabeled monoclonal antibody from Telix Pharmaceuticals targets carbonic anhydrase IX (CAIX), an enzyme that regulates cell proliferation and is highly expressed in many tumor types. (medscape.com)
  • AiViva is a clinical stage biotech company led by a team of seasoned industry experts with proven track record in drug development and commercialization. (tmcnet.com)
  • BAY 2731954 (LOXO-195) is an, oral and selective investigational new drug in clinical development rationally designed for the treatment of patients with cancers that have acquired resistance to initial TRK therapy. (worldpharmanews.com)
  • Dormant breast cancer cells exhibit a distinct gene expression signature regardless of metastatic site. (biomedcentral.com)
  • In this study, we developed an efficient system for isolating rare dormant breast cancer cells in experimental metastases models and performed scRNA-seq comparing gene expression in dormant vs proliferative breast cancer cells. (biomedcentral.com)
  • Our studies suggest that the gene expression signature we identified in disseminated dormant breast cancer cells exists in the primary site and can be utilized to predict which patients are more likely to experience a breast cancer recurrence. (biomedcentral.com)
  • Quantify live cell numbers based on their endogenous esterase activity and plasma membrane integrity using green fluorescence. (biotium.com)
  • Autologous USCs were obtained and characterized, and their capacity to differentiate into urothelial cells (UCs) and smooth muscle cells (SMCs) was tested. (biomedcentral.com)
  • More important, people found that, during the pathogenesis of asthma, smooth muscle cells could differentiate into the migration phenotype. (scirp.org)
  • Researchers used the drug to track how blocking mTOR affected the immune response of mice following H3N2 vaccination. (stjude.org)
  • Rockefeller University researchers have developed TrackerSci, a groundbreaking method for tracking the development and aging of brain cells, which could revolutionize the understanding of neurological diseases and aging. (scitechdaily.com)
  • Researchers at Rockefeller University are taking the same approach with newborn brain cells-but these neonates will keep their ID tags for life, so that scientists can track how they grow and mature, as a means for better understanding the brain's aging process. (scitechdaily.com)
  • Taking advantage of single-cell sequencing technologies, researchers have now profiled rare dormant cancer cells and have identified critical intrinsic signaling pathways that govern dormancy in myeloma and prostate cancer [ 6 , 7 ]. (biomedcentral.com)
  • Fig. 8: Selective deletion of Adra1a from OPCs reduces their proliferation. (nature.com)
  • For additional reagents (including antibodies) on cell cycle analysis or DNA replication, check here . (biolegend.com)
  • Antibodies are produced by B cells to recognize and defend against viruses. (stjude.org)
  • This led us to the B-cell response and evidence that the cross-reactive antibodies provide crucial protection against different flu strains," said first author Rachael Keating, Ph.D., a St. Jude scientist. (stjude.org)
  • Zombie dyes, Tag-it™ Violet, and CFSE-DA can be used on live cells for tracking applications. (biolegend.com)
  • CFSE is used to track lymphocyte migration and proliferation. (sigmaaldrich.com)
  • The finding was surprising because previous research had highlighted a likely role for white blood cells known as CD8+ and CD4+ memory T cells for broadening the immune response against different flu strains. (stjude.org)
  • The development of immune checkpoint blockers (ICB) aimed at enhancing antitumor T-cell responses has revolutionized cancer treatment. (aacrjournals.org)
  • In patients with a known primary malignancy, such as colorectal cancer or islet cell tumors, an initial investigation includes an ultrasonographic examination followed by spiral or multisection, 3-phase, contrast-enhanced CT scanning. (medscape.com)
  • Specifically, she is dedicated to understanding the design principles and developing nanoscale and macroscale biomaterials for medical applications, experimental investigations of cell therapies, and integration across multiple disciplines including materials science, chemistry, medical imaging, and life science. (stanford.edu)
  • The role of cell proliferation as an experimental confounder was tested experimentally by performing the cMINC in the presence of the proliferation-inhibiting drug cytosine arabinoside (AraC), and by a careful evaluation of mitotic events over time. (johnshopkins.edu)
  • Also, marine cell cultures may be more forgiving in terms of temperature, pH and oxygen requirements compared to mammalian cell cultures. (foodingredientsfirst.com)
  • New cells are continuously produced in the adult mammalian brain, a critical process associated with memory, learning, and stress. (scitechdaily.com)
  • Here, in the presence of KL+FL+megakaryocyte growth and development factor (MGDF), which recruits virtually all Lin(-)Sca-1(+)kit(+) bone marrow cells into proliferation and promotes their self-renewal under serum-free conditions, IL-3 and IL-11 revealed an indistinguishable ability to further enhance proliferation. (lu.se)
  • After induction, hADSCs expressed von Willebrand Factor (vWF), the cell marker of ECs. (molvis.org)
  • More research is needed with this mode of action and with cell proliferation, as the two could drive the induction of mutations and subsequent carcinogenicity. (cdc.gov)
  • REC-4881, developed by Recursion, inhibits the MEK pathway, a prime mover in cancer cell biology. (medscape.com)
  • This technique has uncovered shifts in cell production in aging brains and has broader applications for studying cell dynamics across various organs. (scitechdaily.com)
  • Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)
  • Sample suitability indicates whether this reagent can be used to label already fixed tissue sections or single cells in suspension or cell culture. (biolegend.com)
  • There can be symmetrical or asymmetrical division depending on the cell and tissue type. (biolegend.com)
  • Discriminating between these different models requires biological information about the cells and tissue of the brain at the start of the folding process. (elifesciences.org)
  • Notably, the pattern of cells and tissue fibers in the cortex had no influence over these mechanical properties and provided no pre-indication of where the sites of folding would occur. (elifesciences.org)
  • Her postdoctoral research in hydrogel and tissue engineering is complementary to her Ph.D. training in nanoparticles and cell tracking. (stanford.edu)
  • Certain techniques can detect brain activity from specific cells of interest for short-lived experiments in small areas of the brain, either in tissue recently removed from animals or by using probes or optogenetic techniques to capture activity in situ. (neurosciencenews.com)
  • A cell-based tissue-engineered urethra may be an alternative for patients who have complicated long strictures and need urethral reconstruction. (biomedcentral.com)
  • Autologous USCs can be used as an alternative cell source for cell-based tissue engineering for urethral reconstruction. (biomedcentral.com)
  • Another critical element required for successful urethral tissue engineering is the cell source. (biomedcentral.com)
  • Recently, the endocrine activity of adipose tissue cells has been intensively studied. (123dok.org)
  • We also point out a possible contribution of adipose tissue-associated mast cell secre- tory activity to the development of these diseases. (123dok.org)
  • third, whole tissue culture was also performed to further demonstrate the different effect of R- and S-albuterol on migration of airway smooth muscle cells. (scirp.org)
  • After mitosis, the cell will undergo the G 0 /G 1 phase when the cells rest to become ready for the next round of replication. (biolegend.com)
  • as they undergo cell division. (lu.se)
  • We identified a group of genes including Cfh, Gas6, Mme and Ogn that were highly expressed in dormant breast cancer cells present in the bone and lung. (biomedcentral.com)
  • T cells play a critical role in the control of cancer. (aacrjournals.org)
  • Cancer is characterized as the uncontrolled multiplication and spread of the body's particular cells. (researchsquare.com)
  • In clinical trials of patients with TRK fusion cancer, larotrectinib demonstrated an ORR of 75 percent (N=55) (95% CI: 61, 85%), including a 22 percent complete response (CR) rate. (worldpharmanews.com)
  • The goal of this study was to develop the first successful iron labeling method of breast cancer cells derived from patient brain metastases and validate this method with imaging during tumour development. (biorxiv.org)
  • Imaging of PDX cells provides a powerful system to better develop personalized therapies targeting breast cancer brain metastasis. (biorxiv.org)
  • Insight into the mechanisms and pathophysiology of breast cancer brain metastasis have long relied on the use of immortalized cell lines that have been injected intracardially into the left ventricle of the mouse heart in order to selectively grow tumours within the brain. (biorxiv.org)
  • To overcome this challenge, we developed an efficient system to isolate and study rare dormant breast cancer cells from metastatic organs including bones, which represent a major site of metastasis. (biomedcentral.com)
  • After isolation of cells from the long bones, we used single cell RNA-sequencing (scRNA-seq) to profile proliferative and dormant PyMT-Bo1 breast cancer cells. (biomedcentral.com)
  • HADSCs were intraocularly injected into oxygen-induced retinopathy (OIR) mice to check the movement, fusion, proliferation, and prognosis in vivo. (molvis.org)
  • While these cell lines have been well characterized, they do not represent the tumour heterogeneity, metastatic behaviours, and histopathology seen clinically, and are unsuitable for evaluating therapies due to their fast progression in vivo ( 8 , 9 ). (biorxiv.org)
  • This microwell-based circular migration zone NCC function assay (cMINC) was further optimized for tox-icological testing of human pluripotent stem cell (hPSC)-derived NCCs. (johnshopkins.edu)
  • Migration of neural crest cells (NCCs) is one of the pivotal processes of human fetal development. (johnshopkins.edu)
  • Removal of the stopper barrier after cell attachment initiates migration. (johnshopkins.edu)
  • However, the effect of albuterol on the proliferation and migration has never been tested. (scirp.org)
  • Since (R)-, but not (S)-albuterol enhances bronchodilation, we expect the two racemic isomers would also affect proliferation and migration of tracheal cells differentially. (scirp.org)
  • By monitoring migratory properties of airway smooth muscle cells in the presence of albuterol isomers, the different effect of albuterol on proliferation and migration of airway smooth muscle cells is probed. (scirp.org)
  • R-albuterol, but not S-albuterol, inhibited cell migration. (scirp.org)
  • However, the different effects of R- and S-albuterol on smooth muscle cells proliferation and migration have never been tested. (scirp.org)
  • As a kind of well mature pharmaceutical for asthma treatment, it would be very important to distinguish the effect of two different isomers of albuterol on proliferation and migration activities of airway smooth muscle cells. (scirp.org)
  • The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
  • The most important endpoints are proliferation (number of divisions per cell), cell cycle progression and cell death. (unibw.de)
  • Cell divisions (black and pink) are also taken into account, so that it can be traced which cell has divided how often and thus the pedigrees, as well as the cell cycles, can be evaluated. (unibw.de)
  • Furthermore, high-resolution cell division tracking demonstrated that all HSC underwent a minimum of 5 cell divisions, suggesting that long-term repopulating HSC are not compromised by IL-3 stimulation after multiple cell divisions. (lu.se)
  • Some of the main factors influencing the production cost are the cost of serum-free growing medium and the doubling time of the fish cells (i.e. the pace of cell proliferation), she details. (foodingredientsfirst.com)
  • The dyes can also be used to track cell populations in co-culture. (biotium.com)
  • Cao studies how tissues and organs maintain stable populations of cells-a hallmark of health-so he and his team wanted to investigate how different cellular populations develop, and whether these varied neuronal cells decline in the same way or forge different paths. (scitechdaily.com)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • These models are more predictive of clinical outcomes and are superior to cell lines for preclinical drug evaluation and therapeutic strategies. (biorxiv.org)
  • Patient-derived xenografts, or PDXs, have begun to supplant traditional cell lines due to their retention of clinical biomarkers and heterogeneity from the original tumour ( 10 ). (biorxiv.org)
  • 1044 steady DU145 cells) in serum-free DMEM with saracatinib were put into the top chamber. (baxkyardgardener.com)
  • Microscopy with phase contrast also has the advantage that no dyes or other treatments are necessary, allowing the cells to be observed completely undisturbed for several days. (unibw.de)
  • Primary culture of rat airway smooth muscle cells was performed by enzyme digestion. (scirp.org)
  • The cell is the basic functional unit of our body, so changes to the cell essentially underlie virtually every disease and the aging process," says Cao, head of the Laboratory of Single-Cell Genomics and Population Dynamics. (scitechdaily.com)