Proliferation cell membranes clone cells. Medical search. Frequent questions

More and more evidence has proved that RAS signals regulate immune cells' recruitment, activation, and differentiation while assisting tumor cells to evade immune surveillance. (bvsalud.org)
In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)
Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
In normal human subjects a small proportion of peripheral blood T-cells simultaneously express both CD4 and CD8 differentiation antigens. (uninsubria.it)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
Activated T cells induce proliferation and differentiation of resting B cells in vitro through their CD40 molecules and lymphokine receptors. (uthscsa.edu)
Differentiation of CH12.LX cells and proliferation of splenic B cells in response to both forms of CD40L was greatly enhanced by exposure to mIg‐mediated signals, with greatest enhancement seen when cells were cultured with Ag prior to receiving other signals. (uthscsa.edu)
Besides, it also participates in cell proliferation, differentiation, regeneration and motion via binding to different proteins [ 3 ]. (biomedcentral.com)
Receptor tyrosine kinases (RTKs) play fundamental tasks in varied cell functions including proliferation differentiation survival migration and rate of metabolism (16). (exposed-skin-care.net)
Mosaic analyses reveal that Id2a function in facilitating both cell cycle progression and neuronal differentiation in the retina is non-cell-autonomous, suggesting that Id2a functions upstream of the extrinsic pathways that regulate retinogenesis. (biologists.com)
Overexpression of FLI1 in erythroblasts causes inhibition of differentiation and ultimately the development of pre-T cell lymphoblastic leukemia/lymphoma [ 11 ]. (biomedcentral.com)
In this study, we investigated how higher-order chromatin structure modulates differential expression of the human INK4b-ARF-INK4a locus during progenitor cell differentiation, cellular ageing and senescence of cancer cells. (biomedcentral.com)
We found that INK4b and INK4a , but not ARF , are upregulated following the differentiation of haematopoietic progenitor cells, in ageing fibroblasts and in senescing malignant rhabdoid tumour cells. (biomedcentral.com)
During progenitor cell differentiation and ageing, PcG silencer EZH2 attenuates, causing loss of PRC binding and transcriptional activation of INK4b and INK4a . (biomedcentral.com)
Developmentally regulated EZH2 levels are one of the factors that can determine the higher order chromatin structure and expression pattern of the INK4b-ARF-INK4a locus, coupling human progenitor cell differentiation to proliferation control. (biomedcentral.com)
Development and homeostasis require the coordinate regulation of cell proliferation and differentiation. (biomedcentral.com)

Bcl-2 protein blocks apoptosis (programmed cell death), and thereby may contribute to tomorigenesis by prolonging cell survival rather than by accelerating the rate of cell proliferation. (bdbiosciences.com)
Abundant evidence implicates this protein in the suppression of apoptosis in many types of cells. (bdbiosciences.com)
24h after TCR transduction, CD107a expression correlated with low expansion rates due to apoptosis of ADRB3 specific T cells in contrast to CHM1 specific transgenic T cells. (oncotarget.com)
Under experimental normoxic condition (21 % O 2 ), deferasirox (DFX) used at 10 μM for 3 days reduced proliferation, led cell cycle arrest in S and G2-M phases and induced cytotoxicity and apoptosis in U251 and U87 GBM cells. (biomedcentral.com)
Irradiation at a dose of 16 Gy repressed proliferation, cytotoxicity and apoptosis, but only in U251 cells, while no synergy with DFX was observed in either cell line. (biomedcentral.com)
Cell cycles and cell apoptosis were detected with flow cytometry. (biomedcentral.com)
But Stathmin downregulation did not induce significant cell apoptosis. (biomedcentral.com)
Furthermore, knockdown of the Stathmin expression also induced the cell cycle arrest at G2/M phase, but no significantly statistical difference on apoptosis rate in U373 and U87-MG cells. (biomedcentral.com)
In cancer, the PD-1/PD-L1 system prevents the proliferation of T lymphocytes and causes the release of cytokines and cytotoxicity, which leads to the apoptosis of tumor-specific T cells, thereby preventing the immune response to cancer cells. (ac.ir)
Experiments using the MTT assay, cell cycle analysis, apoptosis assay, real-time PCR and western blot were performed to establish the expression levels of related genes. (biomedcentral.com)
The results show that downregulation of Rab21 can significantly inhibit cell growth and remarkably induce cell apoptosis in T98G and U87 cell lines. (biomedcentral.com)
Silencing Rab21 resulted in significantly increased expression of apoptosis-related proteins (caspase7, Bim and Bax) in glioma cells. (biomedcentral.com)
We inferred that Rab21 silencing can induce apoptosis and inhibit proliferation in human glioma cells, indicating that Rab21 might act as an oncogene and serve as a novel target for glioma therapy. (biomedcentral.com)
We found that a significant increase occurred in Rab21 expression in T98G and U87 glioma cell lines and that downregulation of Rab21 using specific-siRNA transfected with two glioma cell lines (T98G and U87) significantly inhibits cell growth and remarkably induces cell apoptosis. (biomedcentral.com)
In addition to inducing necrosis and apoptosis, ROS induces autophagic cell death. (biomedcentral.com)
The aim of this study was to analyze the effects of gga-miR-155 on the proliferation, apoptosis and invasiveness of an MDV-transformed lymphocyte line MSB1 and elucidate the underlying mechanisms. (biomedcentral.com)
While overexpression of gga-miR-155 increased proliferation, decreased the proportion of G1 phase cells relative to that in S and G2 phases, reduced apoptosis rates and increased invasiveness. (biomedcentral.com)
The gga-miR-155 promotes the proliferation and invasiveness of the MDV-transformed lymphocyte line MSB1 and inhibits apoptosis by targeting the RORA gene. (biomedcentral.com)
To this end, we overexpressed and inhibited gga-miR-155 in an MDV-transformed cell line using mimics and inhibitors respectively, and analyzed their growth, proliferation, apoptosis and invasiveness to explore the possible role of gga-miR-155 in MDV-mediated tumorigenesis. (biomedcentral.com)
Furthermore, we predicted and demonstrated that Retinoid Acid Receptor-Related Orphan Receptor Alpha (RORA) is one of the targets of gga-miR-155, and the gga-miR-155 regulated the proliferation, cell cycle, apoptosis and invasiveness of MSB1 cells by targeting RORA. (biomedcentral.com)

In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
New antitumor strategies using drugs targeted to intracellular signaling involved in cell proliferation and survival, angiogenesis, and metastasis have become promising in recent years. (researchgate.net)
B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
Stathmin as a critical protein involved in microtubule polymerization, is necessary for survival of cancer cells. (biomedcentral.com)
Autophagy usually serves as a survival mechanism in response to stress conditions, but excessive induction of autophagy results in cell death. (biomedcentral.com)
In particular, the loss of ALKBH6 in human pancreatic cancer cells increases alkylating agent-induced DNA damage and significantly decreases cell survival. (frontiersin.org)
Overall, our data suggest that ALKBH6 is required to maintain the integrity of the genome and promote cell survival of pancreatic cancer cells. (frontiersin.org)
The extremely low 5-year overall survival of patients is, in part, due to pancreatic cancer cells having several mechanisms of resistance to different chemotherapeutic treatments, one of which is their capacity to efficiently repair alkylating agent-induced DNA damage. (frontiersin.org)
CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand. (biolegend.com)

Autologous as well as allogeneic CD8 + T cells transduced with tumor antigen specific T cell receptors (TCR) may cause significant tumor lysis upon adoptive transfer. (oncotarget.com)
NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
To address whether the expression of the CD8 receptor on the cell membrane was associated to differences in the physiology of the T-cells, we isolated, from the same clone, CD4 single positive (SP) cells from those co-expressing CD4/CD8 receptors (DP cells) and stimulated them in vitro with antigen presenting cells (APC) carrying TSHR. (uninsubria.it)
Long-term engraftment of allogeneic cells necessitates eluding immune-mediated rejection, which is currently achieved by matching for human leukocyte antigen (HLA) expression, immunosuppression, and/or delivery of donor-derived cells to sanctuary sites. (ashpublications.org)
The present study was designed to test the hypothesis that signals delivered via the B cell antigen (Ag) receptor (membrane immunoglobulin, mIg) and major histocompatibility complex (MHC) class II molecules enhance B cell responsiveness to CD40‐mediated signals, providing specificity to the Ag‐nonspecific, MHC‐unrestricted CD40 signal. (uthscsa.edu)
Bishop, GA, Warren, WD & Berton, MT 1995, ' Signaling via major histocompatibility complex class II molecules and antigen receptors enhances the B cell response to gp39/CD40 ligand ', European Journal of Immunology , vol. 25, no. 5, pp. 1230-1238. (uthscsa.edu)
Methods & Materials In this study, the extracellular part of the humanized PD-1 protein was cloned and expressed, and the protein was injected as an antigen into a camel (Camelus dromedarius) to obtain a camel polyclonal antibody against PD-1 protein. (ac.ir)
Antibody- and T-cell-based approaches to targeting of B-cell maturation antigen have shown efficacy. (msdmanuals.com)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation. (rupress.org)
We show that Per8p is an integral membrane protein of the peroxisome and that it is concentrated in the membranes of newly formed organelles. (rupress.org)
Maturation occurs in the cell membrane resulting in a 180-kDa heterodimeric protein composed of a 40-kDa α-chain and a 150-kDa transmembrane β-chain with intrinsic tyrosine kinase activity ( 5 , 6 ). (spandidos-publications.com)
RON is activated in response to macrophage-stimulating protein (MSP), and then induces an invasive program ( 8 ) consisting of cell proliferation, migration, and invasion, all of which are important at multiple points during tumorigenesis. (spandidos-publications.com)
Bcl-2 is an intracellular membrane protein and resides primarily in the nuclear envelope, outer mitochondrial membrane and endoplasmic reticulum. (bdbiosciences.com)
Mouse anti PTEN tumour suppressor antibody, clone A2b1, is specific for PTEN, a tumour suppressor protein located on the human chromosome 10 that is often mutated in various types of advanced cancers. (bio-rad-antibodies.com)
PTEN exhibits protein phosphatase activity and can suppress the growth of glioma cells. (bio-rad-antibodies.com)
Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
Normally, upon the stimulations by growth factors (GFs), the receptor tyrosine kinases (RTKs) are activated, and then recruit PI3K protein complex on the membrane to generate phosphatidylinositol-3,4,5-triphosphate (PIP3) from phosphatidylinositol-4,5-biophosphate (PIP2) [ 2 ]. (biomedcentral.com)
The second messenger PIP3 recruits and activates 3-phosphoinositide dependent protein kinase-1 (PDK1) and AKT serine/threonine kinase on the membrane [ 2 ]. (biomedcentral.com)
Full length human recombinant protein of human CD27 (NP_001233) produced in HEK293T cell. (thermofisher.com)
Expression levels of Stathmin protein in each group were determined by using Western Blot, and the proliferation and migration ability of the cells with downregulated Stathmin were evaluated through CCK8 assay and transwell invasion assay, respectively. (biomedcentral.com)
According to the results from Western Blotting, Stathmin protein expression level decreased significantly in the U373 and U87-MG cells after transfected with pLV3-si-Stathmin, respectively, compared with those transfected with pLV3-NC. (biomedcentral.com)
In glioblastoma cells, the cell proliferation and migration were greatly inhibited after the downregulation of Stathmin protein. (biomedcentral.com)
The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. (origene.com)
This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. (origene.com)
Hosseininejad-Chafi M, Kianmehr Z, Pooshang Bagheri K, Kazemi-Lomedasht F, Behdani M. Expression of Recombinant Human Programmed Cell Death Protein-1 and Development of Camel Polyclonal Antibody. (ac.ir)
Aims Programmed cell death protein-1 (PD-1) is a membrane receptor expressed on the surface of T and B lymphocytes, monocytes, natural killers, and dendritic cells. (ac.ir)
A common heterophilic ligand for these RTK family members is definitely Gas6 a vitamin K-dependent protein that is widely secreted by most cells including the lungs intestine Serping1 and vascular endothelium (43). (exposed-skin-care.net)
Gas6 is the product of growth arrest-specific gene 6 which was in the beginning cloned from serum-starved fibroblasts and shares about CUDC-305 (DEBIO-0932 ) 44% sequence identity and related website organization with protein S a negative regulator of blood coagulation (48). (exposed-skin-care.net)
Over two dozen proteins involved in protein import and membrane insertion, peroxins, encoded by PEX genes, have been characterized. (tcdb.org)
In the budding yeast Saccharomyces cerevisiae , the minimal transport machinery includes the membrane proteins Pex13 and Pex14 and the cargo-protein-binding transport receptor, Pex5. (tcdb.org)
Pex4p is a membrane protein that may facilitate cycling of Pex5p back to the cytosol. (tcdb.org)
Evidence for GO induced autophagy includes increased levels of isoform B-II microtubule-associated protein 1 light chain 3 (LC3), aggregation of green fluorescence protein-tagged LC3, and increased numbers of autophagic vacuoles in cells. (biomedcentral.com)
Protein samples were separated on a 10% polyacrylamide SDS gel and transferred onto nitrocellulose membranes. (liveconscience.com)
CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. (biolegend.com)
Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)
Diagnosis typically requires demonstration of M-protein (sometimes present in urine and not serum but rarely absent entirely) and/or light-chain proteinuria, and excessive plasma cells in the bone marrow. (msdmanuals.com)
In 15 to 20% of patients, plasma cells secrete only Bence Jones protein. (msdmanuals.com)
DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)

Recepteur d'origine nantais (RON) is a receptor tyrosine kinase (RTK) normally expressed at low levels in epithelial cells. (spandidos-publications.com)
Beta-3-adrenergic receptor (ADRB3) as well as chondromodulin-1 (CHM1) are over-expressed in Ewing Sarcoma (ES) but not on T cells. (oncotarget.com)
Since the SLAMF low signature was strongly associated with an unmutated CLL immunoglobulin heavy chain (IGHV) status in large datasets, we investigated the impact of SLAMF1 and SLAMF7 on the B cell receptor (BCR) signaling axis. (nature.com)
In this study we characterized a subset of CD4+ clones, from a healthy donor, that is specific for the thyrotropin receptor (TSHR) and that showed cells co-expressing the CD8 receptor. (uninsubria.it)
The ATP-dependent dislocation of the PTS1 receptor from the peroxisomal membrane into the cytosol is mediated by the AAA peroxins Pex1p and Pex6p ( Platta et al . (tcdb.org)
The interferon-α/β receptor (IFNAR) is a virtually ubiquitous membrane receptor which binds endogenous type I interferon (IFN) cytokines. (wikidoc.org)
IFNAR is a heteromeric cell surface receptor composed of two subunits, referred to as the low affinity subunit, IFNAR1 , and the high affinity subunit, IFNAR2 . (wikidoc.org)
BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)

The human RON gene was originally cloned from keratinocytes ( 1 ). (spandidos-publications.com)
IFNAR1 is the low affinity subunit, originally cloned in 1990, and is composed of four fibronectin type II-like (FNII-like) subdomains, termed SD1-4. (wikidoc.org)

Among these RON variants, RONΔ160 is located at the plasma membrane, whereas RONΔ165 and RONΔ155 are retained in the cytoplasm. (spandidos-publications.com)
Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. (thermofisher.com)

These proteins control the dynamics of the actin cytoskeleton and thus represent key regulatory molecules that are active during cell migration ( 12 ). (spandidos-publications.com)
Mutation at Y317 of p110α reduces the proliferation, migration, and invasion of cancer cells through Src-MLC2 pathway, while mutation at Y508 of p110α impairs AKT signaling. (biomedcentral.com)
Stathmin downregulation affected the biological behaviors of U373 and U87-MG glioblastoma cells, inhibiting the proliferation and migration of tumor cells. (biomedcentral.com)
In the present study, we demonstrated that downregulation of Stathmin expression inhibited the cell proliferation, cell migration and tumor formation in nude mice of U373 and U87-MG glioblastoma cells. (biomedcentral.com)
So, Stathmin affects glioblastoma progression through regulating cell proliferation, cell cycle and cell migration. (biomedcentral.com)
Upon immobilization sAxl advertised cell migration and induced the phosphorylation of Axl and phosphatidylinositol 3-kinase. (exposed-skin-care.net)

Ectodomain dropping has emerged as an important posttranslational mechanism to regulate the functions of various integral membrane-bound proteins including adhesion molecules cytokines growth factors and their receptors (57 60 Both. (exposed-skin-care.net)

Low levels of ROS can promote cell proliferation but high levels induce cell death [ 13 ]. (biomedcentral.com)

So, this study was designed to elucidate the function of Stathmin gene in the tumorigenesis and progression of glioblastoma cells. (biomedcentral.com)

This expression was dependent on activation of either NF-κB, JAK1/JAK2 or BTK pathways since these pathways were activated in tumor B-cells and ex vivo treatment with the inhibitory molecules PHA-408, ruxolitinib and ibrutinib led to decrease of its expression. (springer.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Proteins targeted to the peroxisome (in plants called glyoxisomes) are synthesized in the cytoplasm of the cell and are targeted to the peroxisome post-translationally, possibly via multiple pathways. (tcdb.org)
The activation of both MAPK and PI3K signaling pathways has been implicated in the stimulation of proliferation by IRS-1 [ 11 ]. (biomedcentral.com)

One important insight came from the discovery that the increased motility and invasiveness of cancer cells is reminiscent of the epithelial-mesenchymal transition (EMT) that occurs during embryonic development ( 2 ). (spandidos-publications.com)
Rab23 is implicated as a metastatic or tumorigenic biomarker as it shows differential expression in tumor cells and has been demonstrated to mediate proliferation and invasiveness in tumor cells [ 12 ]. (biomedcentral.com)
Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)

Our results demonstrated high potential of IL-15 for NK cell expansion, while IL-21 triggered NK cell maturation and functionality. (frontiersin.org)

Accordingly, Rad51b-c.92delT variant reduced replication fork progression of patient-derived lymphoblastoid cell lines and pluripotent reprogramming efficiency of primary mouse embryonic fibroblasts. (nature.com)
Id2a-deficient retinas possess increased numbers of cells occupying S phase, at the expense of mitotic cells, and kinetic analyses demonstrate that Id2a is required for S-phase progression and/or the transition from S to M phase. (biologists.com)
Among potential antineoplastic agents, antioxidants are known to exert protective mechanisms on healthy cells that may prevent disease progression. (biomedcentral.com)
The FA2 gene of Chlamydomonas encodes a NIMA family kinase with roles in cell cycle progression and microtubule severing during deflagellation. (bio.net)

Treatment of LMP1/CD40-expressing lymphomatous mice with an anti-PD-L1 monoclonal antibody induced tumor regression with decreased spleen content, activation and proliferation rate of B-cells as well as a marked increase in T-cell activation, as assessed by CD62L and CD44 expression. (springer.com)
To test this hypothesis, both an Ag‐specific mouse B cell clone CH12.LX, and freshly isolated resting splenic B cells were cultured with either soluble or membrane‐bound forms of the T cell ligand for CD40 (CD40L), in the presence or absence of additional signals provided by Ag or anti‐IgM, interleukin‐4, and class II‐specific monoclonal antibody (mAb). (uthscsa.edu)

Tumor cell motility is the hallmark of invasion and an essential step in metastasis. (spandidos-publications.com)
Rab21 is a member of the Rab subfamily, which has been identified as required for tumor-associated fibroblasts to promote the invasion of squamous carcinoma cells. (biomedcentral.com)

Repair of mitomycin-C-induced chromosomal aberrations was impaired in RAD51B/Rad51b-c.92delT human and mouse somatic cells in vitro and in explanted mouse bone marrow cells. (nature.com)
To address this issue, the expression levels of Snail, RhoA and fibronectin, as well as MMP‑2, were reduced in the breast tumor cell lines MDA‑MB‑231 and MDA‑MB‑435S, and their biological responses were studied in vitro and in vivo. (spandidos-publications.com)
The growth and metastasis abilities of cells are evaluated in vitro and in vivo. (biomedcentral.com)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
Soluble Axl (sAxl) is present in cell-conditioned medium of CUDC-305 (DEBIO-0932 ) tumor cells growing in vivo and in vitro and in the sera of humans mice and rats (23 50 However the identities of the sAxl-generating protease(s) and the mechanism(s) that account for this process remain unknown. (exposed-skin-care.net)
This cell line would serve as potent in vitro model for the exploration of the pathogenesis of ESCC. (biomedcentral.com)
Therefore these genotypically unique cell lines likely relevant for malignant mesothelioma formation are expected to serve as useful in vitro models in particular to compare with in vivo studies in mice of the same genotype. (liveconscience.com)

Investigation of the subcellular distribution of the bcl-2 oncoprotein: residence in the nuclear envelope, endoplasmic reticulum, and outer mitochondrial membranes. (bdbiosciences.com)

In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)

Voltage-gated hydrogen channel 1 is a voltage-gated proton channel that has been shown to allow proton transport into phagosomes and out of many types of cells including spermatozoa, electrically excitable cells and respiratory epithelial cells. (wikipedia.org)
In this process, epithelial cells acquire fibroblast-like properties such as the functional loss of E-cadherin. (spandidos-publications.com)

Cell viability of non-cancerous RWPE-1 cells was affected neither by GT3 nor AT. (biomedcentral.com)

RhoA has been implicated in the formation of stress fibers and cell adhesion in fibroblasts. (spandidos-publications.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)

Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)
Using loss-of-function and overexpression assays in zebrafish, we demonstrate that Id2a levels modulate retinoblast cell cycle kinetics and thereby influence neuron and glia formation in the retina. (biologists.com)
Overexpression of IRS-1 resulted in inhibition of basal autophagy, and reduced oxidative stress-induced autophagy and cell death. (biomedcentral.com)
Our results suggest that overexpression of IRS-1 promotes cells growth, inhibits basal autophagy, reduces oxidative stress-induced autophagy, and diminishes oxidative stress-mediated autophagy-dependent cell death. (biomedcentral.com)

Knockdown of autophagy-related gene 5 inhibited basal autophagy and diminished oxidative stress-induced autophagy and cell death. (biomedcentral.com)

Then pLV3-si-Stathmin and pLV3-NC were stably co-transfected into U373 and U87-MG glioblastoma cells. (biomedcentral.com)

The HRP substrate (Millipore Luminata Forte) was incubated for 3?min within the membrane and analyzed on a Western blot reader (FluorChem E System Bucher Biotec Basel Switzerland). (liveconscience.com)

Here, we describe the characterization of one of these mutants, per8, and the cloning of the PER8 gene. (rupress.org)
Translocation of bcl-2 sequences from chromosome 18 onto the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B-cells deregulates bcl-2 gene expression, resulting in high levels of bcl-2 mRNA. (bdbiosciences.com)
The lentiviral interference vector pLV3-si-Stathmin targeting Stathmin gene and the control vector pLV3-NC were established for the co-transfection of 293T cells together with the helper plasmids. (biomedcentral.com)
Furthermore, gga-miR-155 directly targeted the RORA gene and downregulated its expression in the MSB1 cells. (biomedcentral.com)

Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
In this study, we addressed feeder cell-free expansion methods using common γ-chain cytokines, especially IL-15 and IL-21. (frontiersin.org)
In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
Many tumor microenvironments, including lymphoma and carcinoma, are enriched in immune suppressive cells that contribute to immune exhaustion by means expression of inhibitory ligands, suppressive cytokines, and tumor-promoting factors. (researchgate.net)

Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)

IL-6 signals via binding to either the membrane bound IL-6Rα (classic signaling) or soluble (s)IL-6Ra (trans-signaling) that then form a complex with gp130 to activate the JAK/STAT signaling pathway. (jneurosci.org)
Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. (thermofisher.com)
Several studies shown that Axl could mediate cell adhesion and aggregation through homotypic ectodomain associations (9 23 Both CUDC-305 (DEBIO-0932 ) murine and human being Axl proteins undergo proteolytic processing to yield a soluble form of this molecule. (exposed-skin-care.net)
2011) addresses two of these aspects, the translocation of soluble proteins into the peroxisomal matrix and the biogenesis of the peroxisomal membrane. (tcdb.org)
Most peroxisomal membrane proteins do not have PTS1 or PTS2, and insertion requires several peroxins distinct from those required for import of soluble matrix proteins. (tcdb.org)

Deferiprone, a bidentate iron chelator, has been shown to have antiproliferative and cytotoxicity activities in neuroblastoma cell lines [ 7 ]. (biomedcentral.com)

NF-κB, one of the most cited transcription factor in B-cell lymphomas, is able to increase tumor cell expression of PD-L1 either directly or indirectly [ 3 ]. (springer.com)
The present data provide evidence that Snail promotes tumor cell motility and angiogenesis which is mainly mediated through the regulation of RhoA activity. (spandidos-publications.com)
Previous studies reported that several members of Rab GTPase family are involved in the development of glioma, and abnormal expression of Rab small GTPases is known to cause aberrant tumor cell behavior. (biomedcentral.com)
In human malignancies, including glioma, abnormal expression of Rabsled to aberrant tumor cell behavior. (biomedcentral.com)
The karyotype demonstrated tumor cell patterns with aneuploidy and complex chromosomal aberrations. (biomedcentral.com)

We hypothesized lysosome-associated membrane glycoprotein 1 (LAMP1, CD107a) to be a marker for fratricide in TCR transgenic CD8 + T cells. (oncotarget.com)
Platelets play a primary role in this process, interacting with subendothelium-bound von Willebrand factor (vWf) via the membrane glycoprotein (GP) Ib complex. (medscape.com)

Finally, we discussed advances in RAS-targeted immunotherapies, including cancer vaccines and adoptive cell therapies, with a particular focus on combination strategies with personalized therapy and great potential to achieve lasting clinical benefits. (bvsalud.org)
When germ-free cell cultures became a laboratory routine, hopes were high for using this novel technology for treatment of diseases or replacement of cells in patients suffering from injury, inflammation, or cancer or even refreshing cells in the elderly. (hindawi.com)
13 Genome Plasticity and B Cell, University of Paris-Sud, Centre National de la Recherche Scientifique, Cancer Institute Gustave Roussy, Villejuif, France. (jci.org)
Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent malignancies and a major cause of cancer related death worldwide, especially in China. (biomedcentral.com)
Sustainable and uncontaminated cancer cell lines provide available resources for researchers to utilize. (biomedcentral.com)
Cancer cells produce greater levels of ROS than normal cells do because of increased metabolic stresses. (biomedcentral.com)
However, excessive production of ROS kills cancer cells. (biomedcentral.com)
It is not fully understood how cancer cells grow rapidly and survive in the presence of high ROS levels. (biomedcentral.com)
In non-small cell lung cancer, the FLI1 expression score is associated with the stage of SCLC. (biomedcentral.com)
Using this CasIP assay, we identified FECR1, a FLI1 exonic circular RNA that binds to the FLI1 promoter and epigenetically activates FLI1 in breast cancer cells. (biomedcentral.com)
Here, we tested whether ALKBH6 plays a significant role in preventing alkylating DNA damage and decreasing genomic instability in pancreatic cancer cells. (frontiersin.org)
Several alkylating agents are commonly used in chemotherapy but may induce DNA damage to cancer cells as well as normal cells. (frontiersin.org)
The INK4b-ARF-INK4a tumour suppressor locus controls the balance between progenitor cell renewal and cancer. (biomedcentral.com)
The INK4b-ARF-INK4a tumor suppressor locus (Figure 1A ) plays a central role in controlling the equilibrium between progenitor cell renewal and cancer risk [ 1 - 8 ]. (biomedcentral.com)
The effects of GT3 and AT were studied on androgen sensitive LNCaP and androgen independent PC-3 prostate cancer cells. (biomedcentral.com)
arations verified the presence of cancer cells in all samples. (lu.se)
Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)
Multiple myeloma is a cancer of plasma cells that produce monoclonal immunoglobulin and invade and destroy adjacent bone tissue. (msdmanuals.com)

In either methanol or methylamine medium, conditions that normally induce the organelles, per8 cells contain no peroxisome-like structures and peroxisomal enzymes are located in the cytosol. (rupress.org)
2023 ). Imaging fluorescence cross-correlation spectroscopy showed that cargo import correlates with transient focusing of GFP-Pex13 and GFP-Pex14 on the peroxisome membrane. (tcdb.org)
This subcomplex also exists in the peroxisome membrane. (tcdb.org)

The defects suggest faulty specification of different cell types and result in impairment of processes as diverse as cell proliferation and commitment, cell adhesion and cell recognition. (biologists.com)

They are known to be involved in the regulation of NK and T cell responses, mostly by homotypic interactions except for SLAMF2 (CD48) and SLAMF4 that interact with each other [ 10 ]. (nature.com)
The TNF superfamily members are known for the regulation of cell proliferation and death. (thermofisher.com)

The ciliary epithelium, a bilayer of secretory neuroepithelial cells, is the site of synthesis of aqueous humor (inflow system), and it regulates its secretion by the coordinated action of a complex transport system that involves ion channels and active transporters. (yale.edu)
The ciliary epithelium, a bilayer of secretory neuroepithelial cells, is the site of synthesis of aqueous humor (inflow system), and regulates its secretion by the coordinated action of a complex transport system involving ion channels and active ion secretion, from the stromal to the aqueous-humor surface, followed by osmotic water movement. (yale.edu)

However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)
Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
1 Section of Molecular Cell and Developmental Biology, Institute for Cell and Molecular Biology, The University of Texas at Austin, Austin, TX 78722, USA. (biologists.com)

If Extramacrochaetae par-ticipates in cell specification by dimerizing with basic-region-helix-loop-helix proteins, the variety of defects and tissues affected by the insufficiency of extramacrochaetae suggests that helix-loop-helix proteins are involved in many embryonic developmental processes. (biologists.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
Tissues and Cells. (lu.se)

In this study, we characterized the roles of Rab21 (Rab GTPase 21), a member of Rab GTPase family, in glioma cells. (biomedcentral.com)

Clone 4D7 recognizes human Bcl-2. (bdbiosciences.com)
For immunohistochemistry of paraffin-embedded sections, the monoclonal mouse anti-human Bcl-2 (clone Bcl-2/100, Cat. (bdbiosciences.com)
In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
The aim of the present study was, therefore, to investigate the cytostatic and cytotoxic impact of the new iron chelator deferasirox (DFX) on human GBM cells in well-defined clinical situations represented by radiation therapy and mild-hypoxia. (biomedcentral.com)
IOP elevation in human glaucoma results from an obstruction of the outflow system leading to an abnormal increase in eye pressure, death of retinal ganglion cells and loss of vision. (yale.edu)
Human glioma cell lines U251, T98G, SHG44, U87 and U373 were obtained from the Cell Bank of Chinese Academy of Sciences. (biomedcentral.com)
Human cell lines are now widely used in laboratories worldwide. (biomedcentral.com)
PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with CD137 (clone 4B4-1) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram). (biolegend.com)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

Therefore, ex vivo NK cell expansion protocols are currently being developed. (frontiersin.org)
In a proof of concept in vivo study, we also observed a therapeutic effect of adoptively transferred IL-15 expanded and IL-21 boosted NK cells in combination with image guided high precision radiation therapy using a luciferase-transduced RMS xenograft model. (frontiersin.org)
In summary, this two-phased feeder cell-free ex vivo culturing protocol combined efficient expansion and high cytolytic functionality of NK cells for treatment of radiation-resistant RMS. (frontiersin.org)
MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
However, despite constitutive B cell expression of CD40 and lymphokine receptors, widespread nonspecific polyclonal B cell activation by activated T cells is seldom observed in vivo. (uthscsa.edu)

Rabs interact with a lot of effector and affector proteins involved in membrane fusion and directed transport of organelles along cytoskeletal frameworks. (biomedcentral.com)

p15 INK4b and p16 INK4a are closely related proteins and both act on the Rb-pathway through the inhibition of the proliferation-promoting cyclin-dependent kinases CDK4 and CDK6. (biomedcentral.com)
however, inhibition results in a partial block of cell death. (biomedcentral.com)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)

The ability of class II MHC‐mediated signals to enhance Ag‐specific B cell responsiveness to CD40‐mediated signaling may selectively promote the activation of B cell clones capable of cognate interactions with helper T cells. (uthscsa.edu)

To qualify as stem cells, self-renewal has to be shown as well [ 10 ]. (hindawi.com)

Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)

However, direct injection of IL-2 has been shown to be accompanied by severe side effects, such as vascular leak syndrome, activation-induced cell death, and strong induction of regulatory CD4 pos T cells, which did not occur after IL-15 administration ( 15 , 16 ). (frontiersin.org)
Escape from immune control must be important in the natural course of B-cell lymphomas, especially for those with activation of NF-κB. (springer.com)
The pre-clinical LMP1/CD40-expressing transgenic mouse model is characterized by B-cell specific CD40 signaling responsible for NF-κB continuous activation with a spleen monoclonal B-cell tumor after 1 year in 60% of cases. (springer.com)
NF-κB constitutive activation is found either in aggressive diffuse large B-cell lymphomas (DLBCL) with an activated phenotype (ABC-DLBCL), or in indolent B-cell lymphomas such as chronic lymphocytic leukemia, Waldenström Macroglobulinemia, marginal zone B-cell lymphomas (MZL) [ 4 ]. (springer.com)
Here, we wanted to explore the putative interest of PD-L1 immune therapy against B-cell lymphoma with NF-κB activation. (springer.com)
Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)
In B cells, this class of receptors has been shown to be expressed in distinct patterns associated with development and activation [ 11 ]. (nature.com)
RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. (thermofisher.com)
However, the mechanism of action of GT3 in these cells also include a significant activation of ERK which is essential for the apoptotic effect of GT3. (biomedcentral.com)

The membrane bound docking subcomplex includes several Pex proteins, Pex13p, 14p and 17p. (tcdb.org)

There are abundant esophageal squamous cell carcinoma (ESCC) cell lines around the world. (biomedcentral.com)

They can be activated rapidly via germ-line encoded receptors that recognize the presence of stress ligands or absence of self-antigens on target cells ( 1 - 5 ). (frontiersin.org)
Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
A previous study demonstrated that SLAMF1, SLAMF2, and SLAMF7 receptors are rather downregulated on CLL cells as compared to their normal B cell counterpart [ 12 ], suggesting that high expression of these molecules may have detrimental (e.g., antiproliferative) effects in the CLL context. (nature.com)

cSH2 domain S690 phosphorylation decreases p85 binding affinity to tyrosine-phosphorylated proteins resulting in less PI3K membrane localization [ 15 ]. (biomedcentral.com)

In addition, when platelets are activated, negatively charged phospholipids move from the inner to the outer leaflet of the membrane bilayer. (medscape.com)

Molecular simulation is consistent with a water-filled pore that can function as a "water wire" for allowing hydrogen bonded H+ to cross the membrane. (wikipedia.org)
We propose that Per8p is a component of the molecular machinery that controls the proliferation of this organelle. (rupress.org)
however, additional higher molecular weight bands have been observed in some cell types. (bdbiosciences.com)

CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. (thermofisher.com)
High-dose melphalan followed by autologous peripheral blood stem cell transplantation may also be used. (msdmanuals.com)

Two clones were selected to evaluate the test performance of three assays to measure antibodies to Pgp3: multiplex bead assay (MBA), enzyme-linked immunosorbent assay (ELISA), and lateral flow assay (LFA). (cdc.gov)
Overall, each assay demonstrated high accuracy and precision when tested using either clone, and the clones were stable when stored at -20C and 4C for almost 2years. (cdc.gov)

Anatomy37

Organisms13

Diseases2

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes50

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science3

Health Care1

Differentiation19

Apoptosis20

Survival9

Antigen9

Protein30

Receptor8

Originally cloned2

Plasma membrane2

Migration6

Regulate1

Promote cell proliferation1

Tumorigenesis1

Pathways4

Invasiveness3

Maturation1

Progression4

Antibody2

Invasion2

Vitro8

Endoplasmic reticulum1

Malignant2

Epithelial cells2

Viability1

Fibroblasts2

Overexpression4

Oxidative stress1

Glioblastoma cells1

Western Blot1

Gene4

Cytokines4

Characterization2

Soluble5

Cytotoxicity1

Tumor cell5

Glycoprotein2

Cancer18

Peroxisome3

Adhesion1

Regulation2

Regulates2

Progenitor cells3

Biology3

Tissues3

Glioma cells1

Human11

Mammalian1

Vivo5

Proteins involved1

Inhibition2

Bone marrow2

Cognate1

Stem Cells1

Cellular2

Activation9

Bound1

Abundant1

Receptors3

Affinity1

Bilayer1

Molecular3

Peripheral2

Assay2