Proliferation cell cycle or cell. Medical search. Frequent questions

The inhibition of cell proliferation was related to arrest of the cell cycle in the G0/G1 phase and increased apoptosis in response to PTGR1 knockdown as indicated by flow cytometry. (go.jp)
In contrast, no significant alteration of apoptosis in the BCa cells with downregulated LAPTM5 was noticed. (spandidos-publications.com)
Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
In HL-60 leukaemia cells, di-GA activated caspase 3 and dose-dependently induced apoptosis. (nature.com)
These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)
While some assays utilize antibodies to study cell health, proliferation, cell cycle or apoptosis, other types of experiments can rely on non-antibody based methods of assessment, often called non-antibody chemical probes. (biolegend.com)
Cellular division for any cell type is dependent on the inherent function, location and the response of cells to repair, apoptosis, or death. (biolegend.com)
The transcription factor MYC is a proto-oncogene regulating cell proliferation, cell cycle, apoptosis and metabolism. (oncotarget.com)
Through the genome-wide regulation of gene expression, MYC is involved in diverse cellular processes including cell growth, cell cycle, apoptosis, angiogenesis, cell differentiation and genomic instability [ 4 ]. (oncotarget.com)
Therefore, the search for novel agents, which induce apoptosis in cancer cells while displaying limited toxicity towards normal cells, is paramount. (uaeu.ac.ae)
Subsequent analysis of Bcl-2 family proteins and caspase 3/7 activation determined that oleuropein and hydroxytyrosol induced apoptosis in MIA PaCa-2 cells, while oleuropein displayed a protective effect on HPDE cells. (uaeu.ac.ae)
Gene expression analysis revealed putative mechanisms of action, which suggested that c-Jun and c-Fos are involved in oleuropein and hydroxytyrosol induced apoptosis of MIA PaCa-2 cells. (uaeu.ac.ae)
Open up in another window Shape 3 Leucine deprivation induces apoptosis of human being breast tumor BAY 293 cell(A and B) MDA-MB-231 or MCF-7 cells had been incubated in charge (+leu) or leucine-deficient (?leu) moderate for 48h, accompanied by dedication of cleaved caspases inside a and annexin-V assay in B. (C and D) Nude mice. (racetab.org)
We have previously shown the capacity of one PAPU compound, (E)-1-(3,4-dihydroxyphenethyl)-3-styrylurea (PAPU1), to induce caspase-dependent apoptosis in mela-noma cells. (molcells.org)
PAPU1 also induced the mitochondrial-mediated and caspase-depen-dent apoptosis in MCF-7 cells. (molcells.org)
Ganoderma lucidum inhibits proliferation and induces apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
In this study, we investigated the effects of Ganoderma lucidum on cell proliferation, cell cycle, and apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
Furthermore, Ganoderma lucidum induced apoptosis of PC-3 cells with a slight decrease in the expression of NF-kappaB-regulated Bcl-2 and Bcl-xl. (greenmedinfo.com)
These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)
Cell apoptosis was investigated using 4',6-diamidino-2-phenylindole (DAPI), annexin V, and propidium iodide (PI) fluorescence flow cytometry, which was also used to investigate the cell cycle. (medscimonit.com)
Cells are also programmed for death at the genetic level as a response to DNA damages through the process of apoptosis. (bdbiosciences.com)
Cell division, proliferation, and apoptosis and death are integral parts of life. (bdbiosciences.com)
The balance of cell proliferation and apoptosis is important for both development and normal tissue homeostasis. (bdbiosciences.com)
Apoptosis is an organized process that signals cells to self-destruct for cell renewal or to control aberrant cell growth. (bdbiosciences.com)
As cells become damaged or are no longer needed, they undergo apoptosis or programmed cell death, a normal physiological process that occurs during embryonic development and tissue homeostasis. (bdbiosciences.com)
Apoptosis controls the orderly death of damaged cells, whereas necrosis occurs as a result of tissue damage, causing the loss of both damaged and surrounding cells. (bdbiosciences.com)
Methods for detecting apoptosis or dead cells (viability) by cell preparation type. (bdbiosciences.com)
However, some cell types do not display characteristic features of apoptosis. (bdbiosciences.com)
In those cases, multiple aspects of apoptosis might need to be analyzed to confirm the mechanism of cell death. (bdbiosciences.com)
To investigate the effect of gambogic acid (GA) on apoptosis in the HT-29 human colon cancer cell line. (wjgnet.com)
Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling and Hoechst 33342 staining, and quantified by flow cytometry. (wjgnet.com)
End points are proliferation, cell cycle allocation, induction of apoptosis and necrosis. (uni-marburg.de)
Of note, an increased carbohydrate uptake and/or induction of death receptors of cancer cells was exploited to develop glycoconjugates that potentially induce cellular stress, ROS and apoptosis. (degruyter.com)
If DNA damage or abnormalities in spindle formation are detected at these checkpoints, the cell is forced to undergo programmed cell death, or apoptosis . (britannica.com)
For example, mutations in a protein called p53 , which normally detects abnormalities in DNA at the G1 checkpoint, can enable cancer-causing mutations to bypass this checkpoint and allow the cell to escape apoptosis. (britannica.com)
Most changes were subtle, but frequently the genes with altered expression were involved in basal cellular functions such as cell division, cell cycle regulation, apoptosis and cell signalling. (lu.se)
Effect of cadmium on P53 and mitogen-activated protein kinases in a murine macrophage cell line: relation to apoptosis. (cdc.gov)
It is immunotoxic in vivo and induces apoptosis in many cultured cells. (cdc.gov)
Present study was designed to determine the impact of cadmium on cell proliferation, cell cycle and apoptosis, and to investigate the possible involvement of p53 and MAPKs signaling pathways in J774A.1 murine macrophages. (cdc.gov)
Cd inhibited cell proliferation via cell cycle arrest and induced apoptosis in a dose-dependent manner. (cdc.gov)
Results suggested that p53 is not involved in Cd-induced cell cycle arrest and apoptosis in J774A.1 cells. (cdc.gov)
Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)

In recent years, interest in the possible molecular regulators of cell proliferation and differentiation in a wide range of regeneration models has grown significantly, but the cell kinetics of this process remain largely a mystery. (mdpi.com)
In most biological systems the down-regulation of proliferation is necessary for initiation of key steps in the differentiation process. (elsevierpure.com)
We have examined regulatory mechanisms controlling expression of the cell cycle-dependent histone genes, which are functionally coupled with DNA synthesis, in proliferating cells and during the onset of differentiation in several phenotypes including adipocytes. (elsevierpure.com)
Even though its sequence along with its expressional pattern in cancer cell lines are evident, the functional aspects concerning cell proliferation, viability, differentiation and cell cycle regulation of NDRG1 remains vague. (scirp.org)
These results revealed that NDRG1 is functional in prostate cancer cells and able to induce expression of differentiation factors through p53 independent pathway. (scirp.org)
T. Napso, N. Azzam, A. Lerner and F. Fares, " N-myc Downstream Regulated Gene 1 Increases Differentiation Factors Level in Human Prostate Cancer Cells without Affecting Cell Proliferation and Cell Cycle Profiles," Journal of Cancer Therapy , Vol. 4 No. 2, 2013, pp. 568-574. (scirp.org)
A Novel Gene Which Is Up-Regulated during Colon Epithelial Cell Differentiation and Down-Regulated in Colorectal Neoplasms," Laboratory Investigation: A Journal of Technical Methods and Pathology, Vol. 77, No. 1, 1997, pp. 85-92. (scirp.org)
The hallmark of embryonic stem (ES) cells is their ability for self-renewal (capability of unlimited cell division without the loss of pluripotency) as well as for differentiation into all cell types of the adult organism. (uni-muenchen.de)
A high expression of Pes1, Bop1 and WDR12 was observed in ES cells, which strongly decreased during in vitro differentiation. (uni-muenchen.de)
The following methods were applied: at first proliferation assays with photometric analysis were conducted, then an annexin-V-detection was applied for differentiation of the treated cells in vital, necrotic and apoptotic groups. (uni-marburg.de)
On June 8th 2022, a paper titled "Trunk Neural Crest Migratory Position and Asymmetric Division Predict Terminal Differentiation" was published on Frontiers in Cell and Developmental Biology. (biologists.com)
This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. (hindawi.com)
Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
This study was therefore aimed at addressing three key issues: First, is there a dose-dependent effect of EPO on the proliferation and osteogenic differentiation of BMSCs? (hindawi.com)
They inhibit CELL DIFFERENTIATION and induce CELL PROLIFERATION by modulating different CELL CYCLE regulators. (bvsalud.org)
Cancer stem cells are a selective clonal subset of tumour cells that have avoided various cell regulatory mechanisms, including terminal differentiation, and yet have retained the self-renewal properties and proliferative potential of adult stem cells. (who.int)

Unexpectedly, we found that their depletion in primary human lung fibroblasts failed to induce cell cycle arrest but strongly suppressed cell cycle progression. (nih.gov)
We show that the effects on cell cycle progression stemmed from reduced ribosome content and translational capacity, which suppressed the accumulation of cyclins at the translational level. (nih.gov)
It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Some studies showed that blocking cell cycle progression ameliorated fibrosis. (nature.com)
Moreover, TSPAN12 accelerated mitotic progression by controlling cell cycle. (molcells.org)
Cell cycle checkpoints are control mechanisms in the eukaryotic cell cycle which ensure its proper progression. (wikipedia.org)
Each checkpoint serves as a potential termination point along the cell cycle, during which the conditions of the cell are assessed, with progression through the various phases of the cell cycle occurring only when favorable conditions are met. (wikipedia.org)
Progression through these checkpoints is largely determined by the activation of cyclin-dependent kinases by regulatory protein subunits called cyclins, different forms of which are produced at each stage of the cell cycle to control the specific events that occur therein. (wikipedia.org)
As the eukaryotic cell cycle is a complex process, eukaryotes have evolved a network of regulatory proteins, known as the cell cycle control system, which monitors and dictates the progression of the cell through the cell cycle. (wikipedia.org)
Those complexes, in turn, activate different downstream targets to promote or prevent cell cycle progression. (wikipedia.org)
Flow cytometry indicated a decreased G 1 cell cycle progression and the accumulation of cells in the S and G 2 /M phases in GCD2 cells. (elsevierpure.com)
Flow cytometry indicated a decreased G1 cell cycle progression and the accumulation of cells in the S and G2/M phases in GCD2 cells. (elsevierpure.com)
Unpredictably, cell cycle progression, cell proliferation and DNA synthesis were unaffected fol lowing NDRG1 expression. (scirp.org)
However, the pathway downstream NDRG1, involving p21 and c8/18, regulating cell cycle progression and DNA synthesis is unfunctional. (scirp.org)
Flow cytometric analysis showed that PAPU1 blocked cell cycle progression in the G 0 /G 1 phase, and reduced the proportion of cells in G 2 /M phase. (molcells.org)
Increased levels of POGLUT2 could result in the dysregulated immune cell infiltration and tumor microenvironment and showed a significant regulatory effect on the progression of breast cancer through Notch-related signaling. (frontiersin.org)
A series of signal transduction events starting from the activation of cyclin-dependent kinase 2 (CDK2) to binding of E2F -target transcription to phosphorylation of Rb helps maintain cell-cycle progression. (bdbiosciences.com)
Generally, cells have to closely coordinate growth and cell cycle progression during proliferation to prevent premature division. (uni-muenchen.de)
Proliferation and cell cycle progression of the serum-free cultures closely mimicked that of serum-dependent cells. (lu.se)

Taken together, our results suggested that decreased LAPTM5 inhibited proliferation and viability, as well as induced G0/G1 cell cycle arrest possibly via deactivation of ERK1/2 and p38 in BCa cells. (spandidos-publications.com)
Learn about cell viability assays. (abcam.com)
Pancreatic cancer cells (MIA PaCa-2, BxPC-3, and CFPAC-1) and non-tumorigenic pancreas cells (HPDE) were treated with oleuropein, hydroxytyrosol and tyrosol to determine their effect on cell viability. (uaeu.ac.ae)
Outcomes Leucine deprivation decreases viability of tumor cells We 1st determined the result of leucine deprivation on viability of tumor cells. (racetab.org)
Open up in another window Shape 1 The result of leucine deprivation on cell viability(A and B) Cells had been incubated in charge BAY 293 (+leu) or leucine-deficient (?leu) moderate for indicated period, accompanied by MTT assay. (racetab.org)
The MTT assay assessed cell viability, and autophagy was detected by electron microscopy. (medscimonit.com)
Relative cell viability was assessed using MTT assays. (wjgnet.com)
The combination of NV651 and cisplatin, a DNA damage reagent, resulted in a synergistic effect in cell viability, and a significant increase on cell death in HCC cell lines. (lu.se)

Cell cycle flow cytometry assay was used to analyze the effect of G6PC overexpression on the proliferation cycle of liver cancer cells . (bvsalud.org)
Cell cycle flow cytometry showed that G6PC overexpression had significantly inhibited the Huh7 cells G(1)/ S phase transition. (bvsalud.org)
Cellcycle-analysis was applied via flow cytometry. (uni-marburg.de)
Via flow cytometry in both cell lines a clear transformation of cell cycle induced by treatment with the drugs could be demonstrated, but these effects seem to be rather a single effect of flavopiridol. (uni-marburg.de)

The main mechanism of action of the cell cycle checkpoints is through the regulation of the activities of a family of protein kinases known as the cyclin-dependent kinases (CDKs), which bind to different classes of regulator proteins known as cyclins, with specific cyclin-CDK complexes being formed and activated at different phases of the cell cycle. (wikipedia.org)
The E2F gene family is a group of transcription factors that target many genes that are important for control of the cell cycle, including cyclins, CDKs, checkpoint regulators, and DNA repair proteins. (wikipedia.org)
Western blot result showed that G6PC overexpression had down-regulated the proliferation in cell -cycle related proteins expression. (bvsalud.org)
Col-lectively, our results suggest that G 1 cell cycle regulatory proteins and mitochondrial pathways are the crucial targets of PAPU1 in the chemoprevention of breast cancer cells. (molcells.org)
During the first stage called G1, cells monitor their environment and, when the requisite signals are received, the cells synthesize RNA and proteins to induce growth. (bdbiosciences.com)
Several cell cycle-associated proteins, such as Ki-67, are also used as indicators of cell proliferation. (bdbiosciences.com)
During this work, the function of the proteins Pes1, Bop1 and WDR12, which were shown previously to be involved in ribosome biogenesis of mature cell lines, was investigated in mouse ES cells. (uni-muenchen.de)
Localization of the proteins was predominantly nucleolar and the formation of a stable complex (PeBoW-complex), including all three proteins, was experimentally validated in mature mouse cells as well as in mouse ES cells. (uni-muenchen.de)
The proteins that play a role in stimulating cell division can be classified into four groups- growth factors , growth factor receptors , signal transducers, and nuclear regulatory proteins ( transcription factors ). (britannica.com)
Cells use special proteins and checkpoint signaling systems to ensure that the cell cycle progresses properly. (britannica.com)
However, the cell cycle and its checkpoint systems can be sabotaged by defective proteins or genes that cause malignant transformation of the cell, which can lead to cancer . (britannica.com)
Cells depend on their DNA for coding information to make various classes of proteins that include enzymes, certain hormones, transport proteins, and structural proteins that support life. (cdc.gov)
Specialized cell structures called ribosomes are the cellular organelles that actually synthesize the proteins (RNA transcription). (cdc.gov)

Learn about the assays used to assess cell proliferation and the cell cycle. (abcam.com)
Furthermore, POGLUT2 knockdown of breast cancer cells was established, followed by in vitro biological function assays and in vivo tumor growth study. (frontiersin.org)
Analysing the results of the proliferation assays synergistic effects when combining Flavorpiridol and Lonafarnib could not be shown in SKOV3-cells but in BG1-cells. (uni-marburg.de)

Functional analysis revealed that knockdown of PTGR1 significantly inhibited proliferation and colony formation by PC cells. (go.jp)

This protein is a molecular chaperone with specific function in cell signal transduction. (nih.gov)

Furthermore, we established a BCa cell model with downregulated LAPTM5, revealing a significantly delayed growth rate in the BCa cells with knockdown of LAPTM5. (spandidos-publications.com)
Moreover, cell cycle arrest at G0/G1 phase was triggered by decreased LAPTM5 as well, which could lead to delayed BCa cell growth. (spandidos-publications.com)
Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
In turn, the plant produces GA, which seems to combat the improper growth signals and re-establishes cell-cycle control. (nature.com)
Cancer cells obtain a growth advantage through uncontrolled cell proliferation ( Hanahan and Weinberg, 2011 ), which may be caused by mutations that help them adapt to the microenvironment through selective pressure. (molcells.org)
All living organisms are the products of repeated rounds of cell growth and division. (wikipedia.org)
The growth rate of the cells was reduced when grown in ( significantly? (racetab.org)
As there is a substantial growth-inhibitory aftereffect of leucine deprivation, we explored whether leucine deprivation got any inhibitory influence on cell routine development. (racetab.org)
The inhibition of cell growth was also demonstrated by cell cycle arrest at G2/M phase. (greenmedinfo.com)
The article also underscores the various possible mechanisms by which honey may inhibit growth and proliferation of tumors or cancers. (mdpi.com)
Cell proliferation is an increase in the number of cells as a result of growth and division. (bdbiosciences.com)
Pack LR, Daigh LH, Meyer T. Putting the brakes on the cell cycle: mechanisms of cellular growth arrest. (bdbiosciences.com)
One important factor for cell growth is ribosome biogenesis. (uni-muenchen.de)
First, a growth factor must bind to its receptor on the cell membrane . (britannica.com)
Objective: Serum is usually added to growth media when mammalian cells are cultured in vitro to supply the cells with growth factors, hormones, nutrients and trace elements. (lu.se)
Results and Conclusions: The adaptation process was characterized by initial cell population growth. (lu.se)
Results and Conclusions: The adaptation process was characterized by initial cell population growth arrest, and after that extensive cell death, followed by proliferation and long-term survival of clonal cultures. (lu.se)
These agents inhibit cell growth and proliferation. (medscape.com)
The malformations have a normal endothelial cell growth cycle that affects the veins, the capillaries, or the lymphatics, and they do not involute. (medscape.com)

mitotically active cells of intact segments do not significantly contribute to the blastemal cellular sources. (mdpi.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
These data provide new insight concerning the role of autophagy in cell cycle arrest and cellular proliferation, uncovering a number of novel therapeutic possibilities for GCD2 treatment. (elsevierpure.com)
this limitation has been overcome via ectopic expression of human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, to produce large quantities of these cells as an attractive source for cellular transplantation [ 16 - 18 ]. (hindawi.com)
Cell Biochemistry & Function publishes original research articles and reviews on the mechanisms whereby molecular and biochemical processes control cellular activity with a particular emphasis on the integration of molecular and cell biology, biochemistry and physiology in the regulation of tissue function in health and disease . (wiley.com)
Redirection of glutamine to cellular energetics destabilizes glutathione homeostasis in glioblastoma cells and other cancer cells [ 9 ]. (degruyter.com)
In this manuscript, we report the in vitro mutagenicity of waterpipe smoke condensate (WSC), the alteration in cellular parameters of lung alveolar cells in response to WSC exposure and discuss the implication of cellular responses in the pathophysiology of chronic obstructive pulmonary disease (COPD). (who.int)
However, WSC induced cell cycle arrest and cellular senescence mediated by the p53-p21 pathway. (who.int)

Inhibition of ERK suppressed DNA synthesis and had an additive effect with Cd-inhibited proliferation suggesting that Cd-induced ERK activity is not responsible for the G2/M arrest and subsequent inhibition of cell proliferation. (cdc.gov)

Glucose-6 phosphatase catalytic subunit inhibits the proliferation of liver cancer cells by inducing cell cycle arrest]. (bvsalud.org)

Thus, unlike other tumor suppressors, RPL5/RPL11 play an essential role in normal cell proliferation, a function cells have evolved to rely on in lieu of a cell cycle checkpoint. (nih.gov)
In this study, we investigated how TSPAN12 regulates OC cell proliferation both in vitro and in vivo and found that it contributed to tumor proliferation and poor prognosis in this disease through cyclin and cyclin-dependent kinase (CDK) pathways. (molcells.org)
The purpose of BAY 293 our current research would be to investigate the result of leucine deprivation on breast tumor cells and also to elucidate the root mechanisms. (racetab.org)
Four varieties of tumor cells were examined: malignant melanoma A375, lung caner A549, ovarian carcinoma breast and A2780 cancer MCF-7 and MDA-MB-231 cells. (racetab.org)
Open up in another window Shape 2 Leucine deprivation inhibits breasts tumor cell proliferation(A) MDA-MB-231 or CORIN MCF-7 cells had been seeded in 12-well plates and incubated in charge (+leu) or leucine-deficient (?leu) moderate. (racetab.org)
We aimed to determine the signaling pathway regulating cell metabolism and the mechanism related to the regulation of human tumor-associated macrophages (TAMs) in gastric cancer (GC). (cancerindex.org)
POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)
Treatment of mouse NIH3T3 cells with 5FU, a potent inhibitor of rRNA maturation, confirmed an activation of this checkpoint, leading to weak induction of the tumor suppressor p53, induction of the Cdk-inhibitor p21, an increase in active, hypo-phosphorylated Rb, and to accumulation of cells in the G1- and S-phase with an increase of cells in G1-phase. (uni-muenchen.de)

In addition, Plk1 also prevented partial epithelial-mesenchymal transition (pEMT) of tubular epithelial cells via autophagy pathway. (nature.com)
This study investigates the role of impaired proliferation, altered cell cycle arrest, and defective autophagy flux of corneal fibroblasts in granular corneal dystrophy type 2 (GCD2) pathogenesis. (elsevierpure.com)
known to be essential for autophagy) gene knockout cells. (elsevierpure.com)

G6PC inhibits the proliferation, cell -cycle related expression, and migration of liver cancer cells by inhibiting the G(1)/ S phase transition. (bvsalud.org)
Reishi inhibits proliferation and induces programmed cell death in human prostate cancer cells. (greenmedinfo.com)
Our data demonstrate that Ganoderma lucidum inhibits cell proliferation in a dose- and time-dependent manner by the down-regulation of expression of cyclin B and Cdc2 and by the up-regulation of p21 expression. (greenmedinfo.com)
Lonafarnib inhibits the farnesylation of H-Ras and the attachment of Ras to the cell membrane. (uni-marburg.de)
FOXC1 silencing inhibits the epithelial‑to‑mesenchymal transition of glioma cells: Involvement of β‑catenin signaling. (cancerindex.org)
Also inhibits DNA synthesis by blocking incorporation of DNA precursor and inhibiting cell proliferation. (medscape.com)
Inhibits DNA synthesis and thus cell proliferation by causing DNA crosslinks and denaturation of double helix. (medscape.com)
Inhibits topoisomerase II and causes DNA strand breakage, causing cell proliferation to arrest in the late S or early G 2 portion of the cell cycle. (medscape.com)

In proliferating cells transcription of the histone genes is up-regulated at the onset of S phase. (elsevierpure.com)
The decision to commit to a new round of cell division occurs when the cell activates cyclin-CDK-dependent transcription which promotes entry into S phase. (wikipedia.org)
Finally, transcription factors within the nucleus must initiate the transcription of genes involved in cell proliferation. (britannica.com)

On the other hand, the niche notion might coincide with place purpose variation being a latest research discovered that G-CSF in ADSCs can modulate the cell mobility and metabolism of hyaluronan [51]. (translateinthetownships.com)
This indicates the intricate control of metabolism and redox balance for survival of cancer cells that can be targeted by therapeutic agents. (degruyter.com)

A number of techniques are used to assess cell proliferation. (bdbiosciences.com)

This study aimed to investigate the effects of xanthoxyletin, a plant-derived coumarin, on human oral squamous cancer cells in vitro and in mouse xenografts in vivo . (medscimonit.com)
The primary remit of the journal is on mammalian biology both in vivo and in vitro but studies on the biochemistry and function of cells in situ are especially encouraged . (wiley.com)
H-29 cells were used for in vitro experiments in this study. (wjgnet.com)
Flavopiriol blocks the ATP-binding site of cyclin-dependent cinases and leads to a G1- respectively G2-cellcycle arrest in vitro. (uni-marburg.de)
The aim of this study is to investigate potential synergistic effects of Flavopiridol and Lonfarnib inhibiting the proliferation of human ovarian cancer cells in vitro. (uni-marburg.de)
This study supports evidence in literature pointing to a certain activity of these drugs and at the same time emphasizes the different sensibility of human ovarian cancer cell lines to targeted therapeutics in vitro. (uni-marburg.de)
NV556 treatment in an in vitro 3D human liver Extracellular Matrix (ECM) cultured with a TGFβ1-activated human hepatic stellate cell line led to decreased collagen production. (lu.se)

High abundance of glucose in the cytoplasm of a cancer cell also increases flux into other metabolic pathways such as hexosamine biosynthetic pathway (HBP) [ 7 ]. (degruyter.com)

As loss of RPL5/RPL11 abrogates ribosome biogenesis and protein synthesis to the same extent as loss of other essential 60S RPs, we reasoned the loss of RPL5 and RPL11 would induce a p53-independent cell cycle checkpoint. (nih.gov)

The underlying molecular mechanisms by which androgen-independent PC cells acquire the capacity to proliferate remain largely unclear. (go.jp)
In the present study, we examined the anti-proliferative effects of PAPU compounds on MCF-7 human breast cancer cells and determined the molecular mechanisms involved. (molcells.org)
Thus, Ganoderma lucidum exerts its effect on cancer cells by multiple mechanisms and may have potential therapeutic use for the prevention and treatment of cancer. (greenmedinfo.com)
Overall, the results suggest that ES cells use different mechanisms as mature cells to coordinate their proliferation rate with ribosome biogenesis. (uni-muenchen.de)
The mechanisms influenced by Flaoprirol and Lonafarnib are relevant on ovarian cancer cells. (uni-marburg.de)

Further exploration showed that TSPAN12 overexpression accelerated proliferation and colony formation of OVCAR3 and SKOV3 OC cells. (molcells.org)
Knockdown of TSPAN12 expression in A2780 and SKOV3 cells decreased both proliferation and colony formation. (molcells.org)

When the genetic information containing the "blueprint" for these substances is disrupted, cell homeostasis is disrupted, resulting in a wide-range of immediate and/or delayed toxicological effects. (cdc.gov)

Analysis of the changes of migration and invasion, showed significant reduced LAPTM5 suppressed cell metastasis. (spandidos-publications.com)
Overexpression of cyclophilins has been observed in HCC increasing proliferation, metastasis and promoting chemoresistance. (lu.se)

Cell proliferation and clone formation assay were used to observe the proliferation of liver cancer cells . (bvsalud.org)
Transwell and scratch assay were used to observe the invasion and migration of liver cancer cells . (bvsalud.org)
Huh7 and SK-Hep1 cells proliferation assay showed that the number of proliferating cells in the AdG6PC group was significantly lower than the other two groups (P (bvsalud.org)
Clone formation assay showed that the number of clones was significantly lower in AdG6PC than the other two groups (P liver cancer cells . (bvsalud.org)
Transwell assay showed that the number of cell migration was significantly lower in AdG6PC than the other two groups (P (bvsalud.org)

The cell cycle checkpoints play an important role in the control system by sensing defects that occur during essential processes such as DNA replication or chromosome segregation, and inducing a cell cycle arrest in response until the defects are repaired. (wikipedia.org)

Cyclin A2, Cyclin D1, Cyclin E2, CDK2, and CDK4) were significantly involved in the regulation of cell cycle downstream of TSPAN12. (molcells.org)
Scratch repair rate was significantly lower in AdG6PC than the other two groups (P liver cancer cells . (bvsalud.org)
The proliferation rates of homozygous (HO) GCD2 corneal fibroblasts at 72 h, 96 h, and 120 h were significantly lower (1.102 ± 0.027, 1.397 ± 0.039, and 1.527 ± 0.056, respectively) than those observed for the wild-type (WT) controls (1.441 ± 0.029, 1.758 ± 0.043, and 2.003 ± 0.046, respectively). (elsevierpure.com)
p21 and p53 expression was also significantly lower in GCD2 cells compared to the WT. (elsevierpure.com)
Xanthoxyletin significantly inhibited the proliferation of oral cancer cells (IC₅₀, 10-30 µM) with lower cytotoxicity for normal cells. (medscimonit.com)

During EMT, cells will undergo transformation from epithelial phenotype to mesenchymal phenotype ( 14 ) and many characteristics of cells will change including loss of cell-cell adhesion and acquisition of aggressive and metastatic ability ( 15 ). (spandidos-publications.com)
After mitosis, the cell will undergo the G 0 /G 1 phase when the cells rest to become ready for the next round of replication. (biolegend.com)
Most cancer cells undergo metabolic reprogramming that support their survival and proliferation. (degruyter.com)
as they undergo cell division. (lu.se)
Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)

Western blot was used to detect the effect of G6PC overexpression on the cell -cycle protein expression in liver cancer cells . (bvsalud.org)
Cyclophilin overexpression is a common event in fibrotic tissues playing a key role in different stages of the fibrotic process, including inflammation, hepatocyte death, and activation of the hepatic stellate cells leading to increased collagen production. (lu.se)

Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. (spandidos-publications.com)
LAPMT5 is a lysosomal membrane protein preferentially expressed in immune cells ( 5 , 6 ) and hematopoietic cells ( 7 ), having a close interaction with the Nedd4 ( 8 ), a member of the E3 ubiquitin ligases family ( 8 ). (spandidos-publications.com)
Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. (nih.gov)
Cycloheximide, an inhibitor of protein synthesis, did not alter Cd-induced cytotoxicity indicating that J774A.1 cell death by Cd is independent of de novo protein synthesis including p53. (cdc.gov)
DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)

2022, 23,13 ofFascinatingly, it was also found to manage proliferation and self-renewal of grownup neural stem cells as being a crucial mechanism for JAK/STAT and ERK pathway activation [49]. (translateinthetownships.com)
One factor supposed to be involved in self-renewal is the rapid proliferation rate of ES cells, which is coupled to an unusual cell cycle distribution with the majority of cells in S-phase and a very short G1-phase. (uni-muenchen.de)
Thus, the PeBoW-complex seems to be an essential factor for the rapid proliferation of ES cells and might therefore also be involved in self-renewal. (uni-muenchen.de)
Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)

Macrophages are essential inflammatory cells which regulate the features of immune reactions within tumors. (cancerindex.org)

Expression, gene mutation and amplification, methylation, and copy number alteration (CNA) of POGLUT2 were evaluated using The Cancer Genome Atlas (TCGA), Cancer Cell Line Encyclopedia (CCLE), and Genotype-Tissue Expression (GTEx) databases. (frontiersin.org)
This mini review highlights the development of compounds that target cancer cells by taking advantage of redox or metabolic alteration in cancer cells. (degruyter.com)

Temperature-Induced uncoupling of cell cycle regulators. (nih.gov)

Several pathways involved in cell cycle and DNA repair were affected upon NV651 treatment. (lu.se)
A number of direct and indirect radiation interaction pathways can produce damage to the DNA of irradiated cells. (cdc.gov)

Furthermore, previous studies suggested that knockdown of LAPTM4B , another important subtype of the LAPTM family inhibited proliferation of hepatocellular carcinoma ( 11 ), prostate ( 12 ) and breast cancer cells ( 13 ). (spandidos-publications.com)

When conditions are right, cells enter the S stage of the cell cycle and commit to DNA synthesis and replicate their chromosomal DNA. (bdbiosciences.com)
During the synthesis (S) phase of the cell cycle, DNA polymerases incorporate a variety of nucleosides (deoxyadenosine, deoxyguanosine, deoxycytidine and thymidine) into the newly extending strands of DNA. (bdbiosciences.com)
Consequently, ROS-modulation has emerged as an anticancer strategy with synthesis of various ROS-inducing or responsive agents that target cancer cells. (degruyter.com)

Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)
Downloaded from periodic thymic colonization by progenitor cells guarantees stage. (lu.se)
Malignant transformation and uncontrolled proliferation of an abnormally differentiated, long-lived hematopoietic progenitor cell results in a high circulating number of blasts, replacement of normal marrow by malignant cells, and the potential for leukemic infiltration of the central nervous system (CNS) and testes. (msdmanuals.com)

We also assessed the effect of WSC on the expression of genes involved in cell cycle arrest and inflammation. (who.int)

Hence, ribosomal stress does not lead to checkpoint activation via the p53-p21-Rb pathway in ES cells. (uni-muenchen.de)
We could confirm the increased polyploidy upon Mps1 inhibition in neuroblastoma cell lines and PDX model, leading to mitotic catastrophe activating the caspase-dependent mitochondrial apoptotic pathway. (lu.se)

We have previously demonstrated that Ganoderma lucidum down-regulated the expression of NF-kappaB-regulated urokinase plasminogen activator (uPA) and uPA receptor (uPAR), which resulted in suppression of cell migration of highly invasive human breast and prostate cancer cells. (greenmedinfo.com)

Amputation-induced proliferation occurred predominantly within the epidermal and intestinal epithelium, as well as wound-adjacent muscle fibers, where clusters of cells at the same stage of the cell cycle were found. (mdpi.com)
Treatment with PAPU compounds inhibited predominantly proliferation in these cells, where the PAPU1 was the most efficient form. (molcells.org)

To investigate the effects of glucose-6-phosphatase catalytic subunit (G6PC) recombinant adenovirus on proliferation and cell cycle regulation of liver cancer cells . (bvsalud.org)

The use of human telomerase reverse transcriptase-immortalized bone marrow mesenchymal stromal cells (hTERT-BMSCs) as vehicles to deliver antinociceptive galanin (GAL) molecules into pain-processing centers represents a novel cell therapy strategy for pain management. (hindawi.com)
Bone marrow stem cells, including the pluripotent hematopoietic stem cells (HSCs) and bone mesenchymal stem cells (BMSCs), are being considered as potential targets for cell and gene therapy-based approaches against a variety of different diseases. (hindawi.com)
The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)

and syncytial blastoderm mitotic cell cycle. (nih.gov)

Compared to the eukaryotic cell cycle, the prokaryotic cell cycle (known as binary fission) is relatively simple and quick: the chromosome replicates from the origin of replication, a new membrane is assembled, and the cell wall forms a septum which divides the cell into two. (wikipedia.org)
As a biologically important example we have studied the complex formed by cyclins and cyclin-dependent kinases (CDKs), which play an essential role in the control of the eukaryotic cell cycle. (lu.se)

SKOV-3 and BG1 were used as ovarian cancer cell lines. (uni-marburg.de)

Taken together, our study establishes crucial roles for the AtASF1A and AtASF1B genes in chromatin replication, maintenance of genome integrity and cell proliferation during plant development. (nih.gov)

The olive biophenols, oleuropein, hydroxytyrosol and tyrosol, have displayed cytotoxicity towards cancer cells without affecting non-tumorigenic cells in cancers of the breast and prostate. (uaeu.ac.ae)
These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)

Fluorescent or nonfluorescent cytoplasmic proliferation dyes can also be used as a measure cell proliferation. (bdbiosciences.com)

50], in which IL-15 was found for being differentially expressed in distinct subsets of stromal cells akin to this examine. (translateinthetownships.com)

Drosophila CIAPIN1 homologue is required for follicle cell proliferation and survival. (nih.gov)
Pretreatment with PD98059 increased Cd-induced cytotoxicity suggesting that ERK activation may be a survival response in the present cell system. (cdc.gov)

Nonetheless, a shared attribute amongst them is inside the recruitment of specialised immune cells. (translateinthetownships.com)
In inflammatory conditions, these molecules are secreted from cells to mediate the migration of immune cells toward the site of interest. (translateinthetownships.com)
The presence of chemotactic molecules enveloped within extracellular vesicles may have less of the chemoattractant property as being a bypass of surface receptors on immune cells, consequently increasing the chance of EV interaction with other cells by membrane fusion events. (translateinthetownships.com)
Indeed, the potent pathotropic migratory properties of BMSCs and ability to circumvent both the complications associated with immune rejection of allogenic cells and many of the moral reasons associated with embryonic stem cell use suggest that BMSCs are most promising stem cells as a potential target for the clinical use of genetically engineered stem cells [ 14 , 15 ]. (hindawi.com)
Neutrophils are the first immune cells that infiltrate the damaged tissue as early as 24 h after injury. (springer.com)
however, direct antiproliferative effects against malignant cells and modulation of host immune response may play important roles. (medscape.com)

In the final stages of the process, dying cells become fragmented into apoptotic bodies and consequently are eliminated by phagocytic cells without significant inflammatory damage to surrounding cells. (bdbiosciences.com)

Moreover, some studies demonstrated that LAPTM5 was highly expressed in malignant B lymphomas and involved in B cell malignancies ( 10 ), involving in negative regulation of cell surface T and B cell receptor by promoting lysosome degradation ( 6 ). (spandidos-publications.com)

We have identified a histone H4 proximal promoter element, designated Site II, that mediates cell cycle transcriptional control. (elsevierpure.com)

For additional reagents (including antibodies) on cell cycle analysis or DNA replication, check here . (biolegend.com)

Here, an hTERT-BMSCs/Tet-on/GAL cell line was constructed using a single Tet-on-inducible lentivirus system, and subsequent experiments demonstrated that the secretion of rat GAL from hTERT-BMSCs/Tet-on/GAL was switched on and off under the control of an inducer in a dose-dependent manner. (hindawi.com)
The construction of this cell line is the first promising step in the regulation of GAL secretion from hTERT-immortalized BMSCs, and the potential application of this system may provide a stem cell-based research platform for pain. (hindawi.com)

The affect for cross-cell communication with out stimulating chemotaxis is an intriguing mode, which may be utilised a lot more in alignment with intracellular signalling or to provide comparable or other cytokine molecules. (translateinthetownships.com)
To make the decision of whether to enter the cell cycle or not, cells integrate information from a variety of intracellular and extracellular signals. (bdbiosciences.com)

In mature cells, disruptions in ribosome biogenesis are directly linked to the cell cycle machinery by a p53-dependent activation of the G1/S-phase checkpoint, leading to an arrest of cells in G1-phase. (uni-muenchen.de)
Moreover, a putative crosstalk between ribosome biogenesis and proliferation of ES cells was assessed. (uni-muenchen.de)
Impaired ribosome biogenesis is known to activate a p53-dependent checkpoint in mature cell lines, which leads to an arrest of cells in G1-phase. (uni-muenchen.de)

G 2 is a checkpoint stage of interphase where the cell has two sets of dsDNA and must commit to mitosis. (biolegend.com)
The G1 checkpoint, also known as the restriction point in mammalian cells and the start point in yeast, is the point at which the cell becomes committed to entering the cell cycle. (wikipedia.org)
This is linked to the lack of a functional G1/S-phase checkpoint, which allows the cells to enter the S-phase almost directly after mitosis. (uni-muenchen.de)

Anatomy12

Organisms6

Diseases3

Chemicals and Drugs55

Analytical, Diagnostic and Therapeutic Techniques and Equipment13

Phenomena and Processes49

Disciplines and Occupations1

Information Science3

Apoptosis42

Differentiation19

Progression19

Viability8

Flow Cytometry4

Proteins13

Assays3

Inhibited proliferation and colony formation1

Signal Transduction1

Growth19

Cellular8

Inhibition of cell proliferation1

Inducing cell cycle arrest1

Tumor8

Autophagy3

Inhibits8

Transcription3

Metabolism2

Assess cell proliferation1

Vitro7

Cytoplasm1

Induce1

Mechanisms5

Colony formation2

Homeostasis1

Metastasis2

Assay5

Checkpoints1

Significantly5

Undergo5

Overexpression2

Protein6

Self-renewal4

Tumors1

Alteration2

Regulators1

Pathways2

Knockdown1

Synthesis3

Progenitor3

Genes involved1

Pathway2

Prostate cancer1

Predominantly2

Subunit1

Bone marrow3

Mitotic cell cycle1

Eukaryotic cell cycle2

Ovarian1

Chromatin1

Cancers2

Cytoplasmic1

Stromal cells1

Survival2

Immune6

Apoptotic1

Negative regulation of cell1

Histone1

Reagents1

BMSCs2

Intracellular2

Ribosome3

Checkpoint3