Proliferation cell biology clone cells. Medical search. Frequent questions

The method of PCL thus rejuvenates old cells without de-differentiation and passage through an embryonic, pluripotent, stage. (wikipedia.org)
PCL consists in introducing a somatic adult or senescent cell nucleus or entire cell with enlarged membrane pores in an (activated) oocyte and to withdraw this treated cell before its de-differentiation and first cell division occurs. (wikipedia.org)
Using PCL cell de-differentiation and its age reprogramming might be, at least partially, separable. (wikipedia.org)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
The shortage of donors and the potential of universal HLA-null platelet products have stimulated research on the ex vivo differentiation of human pluripotent stem cells (hPSCs) to platelets. (hokudai.ac.jp)
Receptor tyrosine kinases (RTKs) play fundamental tasks in varied cell functions including proliferation differentiation survival migration and rate of metabolism (16). (exposed-skin-care.net)
TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12. (biolegend.com)
The transcription factor STAT1 plays a critical role in modulating the differentiation of CD4 + T cells producing IL-17 and GM-CSF, which promote the development of experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS). The protective role of STAT1 in MS and EAE has been largely attributed to its ability to limit pathogenic Th cells and promote Tregs. (jci.org)
TGF-β, IL-6, and IL-23 promote the differentiation of Th17 cells. (jci.org)
SETD2 loss causes growth arrest and differentiation of AML cells, and leads to increased DNA damage. (nature.com)
Expression of MLL-fusions enhances proliferation and blocks myeloid differentiation of hematopoietic progenitor cells, leading to their pathological accumulation. (nature.com)
Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)
However, in differentiated cells, loss of HP1β has the opposite effect, perturbing maintenance of the differentiation state and facilitating reprogramming to an induced pluripotent state. (biomedcentral.com)
We demonstrate an unexpected duality in the role of HP1β: it is essential in ESCs for maintaining pluripotency, while it is required for proper differentiation in differentiated cells. (biomedcentral.com)
This unique duality makes them an attractive system for potential regenerative medicine and cell therapies, but also for differentiation studies in vitro and for modeling diseases. (biomedcentral.com)
Wnt signaling intimately interacts with the BMP and Notch cascades to drive proliferation and inhibit differentiation in intestinal crypts and adenomas (17, 20). (hubrecht.eu)
To my knowledge this process is currently beyond the capacity of our current cell culture techniques, as B-cell differentiation is controlled by an elaborate cell-signaling process, and even if it was feasible, it would remain impractical for several reasons. (stackexchange.com)
Among these, the neoplastic tumor-suppressor genes (nTSGs) have been shown to fulfill two significant functions requisite for organ size control: first, establishing apicobasal cell polarity as a principle of epithelial tissue organization and appropriate timing of terminal differentiation and, second, exerting cell-proliferation control as a primary factor in tissue growth. (fsu.edu)
In summary, the three major areas of research in my lab are independent yet connected-proper differentiation of follicle cells is key to the establishment of oocyte polarity, and cell competition involves intercellular communication and regulation of cell proliferation. (fsu.edu)
Through the study of temporal and spatial regulation of important genes and pathways in our model systems, we will develop a more comprehensive picture of the molecular mechanisms orchestrating egg development and gain a deeper understanding of how basic cellular functions such as proliferation, differentiation, and growth are regulated in development. (fsu.edu)
Excessive proliferation of keratinocytes, abnormalities in the differentiation process, and continuous shedding of the thickened epidermis are characteristics of psoriasis (Figures 1 (a) and 1 (b)) [ 2 ]. (hindawi.com)
In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)
Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
Thus, exogenous AhR induced catecholaminergic differentiation in N2a-Rα cells. (biomedcentral.com)
The excessive activation of AhR resulted in neural differentiation of Neuro2a cells. (biomedcentral.com)
Activated AhR may disrupt the strictly regulated brain formation with irregular differentiation occurring rather than cell death. (biomedcentral.com)
In-depth study of small non-coding RNA molecules confirmed that microRNA (miR) has an important regulatory role in cell proliferation, differentiation, metabolism, apoptosis and development processes ( 3 ). (spandidos-publications.com)
function of proliferative responses provoked in the induction cell products favor immediate type hypersensitivity reactions, phase of contact allergy, the murine local lymph node assay serving as growth and differentiation factors for mast cells and (LLNA), has been accepted as a stand-alone method for skin eosinophils, the cellular effectors of the clinical manifestations of sensitization testing (NIH, 1999). (cdc.gov)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis. (biomedcentral.com)
Retroviral vectors expressing short hairpin RNAs (shRNAs) against CDC2 caused efficient deletion of CDC2, cellular G2/M arrest concluding in apoptosis and inhibition of proliferation in human glioma cells U251 and SHG-44 cell lines ex vivo. (biomedcentral.com)
In the mosaic Drosophila wing imaginal discs, mahj-/- or lgl-/- cells adjacent to wild-type cells undergo apoptosis, whereas mahj-/- or lgl-/- cells that are not adjacent to wild-type cells do not. (fsu.edu)
The nonautonomous apoptosis in these mutant cells is suppressed by inhibition of the JNK pathway. (fsu.edu)
Furthermore, overexpression of Mahj in lgl-/- mutant cells suppresses JNK activation and blocks apoptosis of lgl-/- mutant cells in the wild-type wing-disc epithelium. (fsu.edu)
G) Quantification of apoptotic cells in the mahj1 mosaic wing discs showing non-cell-autonomous apoptosis in mahj−/− cells. (fsu.edu)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
The degree of methylation affects the expression of target genes and has a significant impact on cell proliferation, apoptosis, cytokine secretion, and cell activity ( 6 , 7 ). (frontiersin.org)
IM combined with IR can synergistically inhibit the proliferation, apoptosis, clonogenesis, invasion, migration and cell cycle of GIST cells. (researchsquare.com)

Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
Expression of KLRB1C/NKR-P1C protein is correlated with the ability to lyse tumor cells in vitro and to mediate rejection of bone marrow allografts. (bdbiosciences.com)
Soluble Axl (sAxl) is present in cell-conditioned medium of CUDC-305 (DEBIO-0932 ) tumor cells growing in vivo and in vitro and in the sera of humans mice and rats (23 50 However the identities of the sAxl-generating protease(s) and the mechanism(s) that account for this process remain unknown. (exposed-skin-care.net)
This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing. (biolegend.com)
Lgr5 resides in Wnt receptor complexes and mediates signaling of the Wnt-agonistic R-spondins (31), explaining the unique dependence of Lgr5 stem cells on secreted R-spondins in vivo and in vitro. (hubrecht.eu)
Additionally, MarinBio specializes in in vitro cell-based assay testing services for clinical and pre-clinical studies, including method development, qualification, validation, transfer, lot-release testing, and potency testing according to guidelines from both FDA and International Council for Harmonization (ICH). (marinbio.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
This study examines the effect of RPL5 on the progression of colon adenocarcinoma (COAD), including cell proliferation, migration and determines its underlying molecular mechanism using CRC cell lines in vitro. (biomedcentral.com)
The DNMT3A-mutated AML cells attenuated M1 macrophage polarization and resisted its killing effect in vitro and in vivo . (frontiersin.org)
In this report the role of versican was examined in adhesion of EOC spheroids and cells to peritoneal mesothelial cell monolayers in vitro as well as in formation of peritoneal tumors using an in vivo xenograft mouse model. (biomedcentral.com)
Forty-two of 104 tetramer pos T-cell clones, isolated from 16 of 17 UCB samples, showed male-specific lysis in vitro. (ashpublications.org)
Under in vitro conditions, changes in the proliferation and invasion ability were detected in myeloma cells. (spandidos-publications.com)
We transfected human and rabbit peripheral blond mononaclear cells (PBMC) with the ACH molecular clone of human T-cell lymphotropic virus type 1 (HTLV-1) to study its in vitro and in vivo properties. (wustl.edu)
This clone will facilitate investigations into the role of viral genes on biological properties of HTLV-1 in vitro and in vivo. (wustl.edu)
In vitro, CCK8, transwell, wound healing and colony formation assays were conducted to explore the biological functions of METT7A in cancer cell. (bvsalud.org)

Downregulation of CDC2 could potentialy inhibit human gliomas cells growth ex vivo and in vivo. (biomedcentral.com)
The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. (biolegend.com)
They can inhibit the growth of tumor cells, kill them via phagocytosis, and promote anti-tumor immunity. (frontiersin.org)
Imatinib is specifically designed to inhibit the tyrosine kinase activity of bcr-abl kinase in Ph1-positive leukemic CML cell lines. (medscape.com)
It inhibits 16 of 18 imatinib-resistant forms of Bcr-Abl expressed in murine myeloid cell lines, but does not inhibit T315I and V299L mutant cells. (medscape.com)

Long-term clonal culturing of organoids from Lgr5 stem cells. (hubrecht.eu)
However, in principle, there is no impediment to removing B-cell progenitors in an individual, conditioning them to produce antibodies (which would be an artificial analogue of the natural process of 'Clonal Selection'), and re-injecting these differentiated B-cells into the patient. (stackexchange.com)
There is a means of growing B-cells in culture to produce antibodies of precise specificity (i.e. monoclonal antibodies - note that clonal selection occurs in a living host for this technique, the B-cells are subsequently harvested then immortalized in culture). (stackexchange.com)
Multiple myeloma (MM) is characterized by the clonal expansion of malignant plasma cells within the bone marrow. (oncotarget.com)
In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
Abnormal expression and mutation of the RBPJ gene may be involved in CD59 clonal proliferation in paroxysmal nocturnal hemoglobinuria. (cdc.gov)

This expression was dependent on activation of either NF-κB, JAK1/JAK2 or BTK pathways since these pathways were activated in tumor B-cells and ex vivo treatment with the inhibitory molecules PHA-408, ruxolitinib and ibrutinib led to decrease of its expression. (springer.com)
Treatment of LMP1/CD40-expressing lymphomatous mice with an anti-PD-L1 monoclonal antibody induced tumor regression with decreased spleen content, activation and proliferation rate of B-cells as well as a marked increase in T-cell activation, as assessed by CD62L and CD44 expression. (springer.com)
NF-κB, one of the most cited transcription factor in B-cell lymphomas, is able to increase tumor cell expression of PD-L1 either directly or indirectly [ 3 ]. (springer.com)
In this study, we reveal from the gene expression profiles of several MKCL clones that the proliferation arrest is correlated with the expression levels of specific cyclin-dependent kinase inhibitors. (hokudai.ac.jp)
Retroviral vectors expressing short hairpin RNAs (shRNAs) targeted against CDC2 were designed and transducted into human glioma cell line ex vivo in order to downregulate the expression of CDC2. (biomedcentral.com)
A previous study demonstrated that SLAMF1, SLAMF2, and SLAMF7 receptors are rather downregulated on CLL cells as compared to their normal B cell counterpart [ 12 ], suggesting that high expression of these molecules may have detrimental (e.g., antiproliferative) effects in the CLL context. (nature.com)
CD8 expression on mature T cells is non-overlapping with CD4. (biolegend.com)
Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. (biolegend.com)
TotalSeq™-D reagents are designed to profile protein expression at single cell level. (biolegend.com)
Conversely, the expression of constitutively active AKT (myr-AKT) overcame the effects of 4HPR and β-catenin-silencing on cell migration. (biomedcentral.com)
In addition, we found that BMP-2, a 4HPR target with antiangiogenic activity, decreased prostate cancer cell proliferation, migration and invasion by down-regulating the pathway described involving AKT phosphorylation, β-catenin stability and cyclin D1 expression. (biomedcentral.com)
They play a crucial role in cell-based assays in drug discovery , focusing on signaling pathways, gene expression , among other endpoints. (marinbio.com)
Fugu B7-H1/DC inhibited T cell proliferation concomitant with increasing levels of both IL-10 and IFN-γ expression, whereas both B7-H3 and B7-H4 promoted T cell growth following IL-2 induction and the suppression of IL-10. (aai.org)
NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
The expression level of RPL5 in colon cancer tissues and cell lines was significantly higher than that in adjacent tissues and NCM460 cells, respectively, and its expression level was higher in HCT116 cells and RKO cells. (biomedcentral.com)
The activation of exogenous AhR in N2a-Rα cells was confirmed using RNAi, with si-AhR suppressing the expression of exogenous AhR. (biomedcentral.com)
Morphologically, N2a-Rα cells bore spontaneous neurites exhibiting axon-like properties with the localization of NF-H. In addition, cdc42 expression was increased in comparison to the control cell line. (biomedcentral.com)
After the co-culture, the increase in pro-inflammatory cytokine expression in the mutant cells was significantly lower than that in the control group, while that in immunosuppressive factors was not significantly different. (frontiersin.org)
Resistin significantly promoted the expression of inflammatory proteins in AML cells. (frontiersin.org)
Furthermore, spheroids with reduced expression of versican failed to disaggregate to complete monolayers when seeded atop monolayers of peritoneal mesothelial cells. (biomedcentral.com)
Failure of spheroids lacking versican to disaggregate as efficiently as controls could be attributed to a reduced cell migration that was observed in the absence of versican expression. (biomedcentral.com)
Importantly, both spheroids and cells with reduced expression of versican demonstrated significantly impaired ability to generate peritoneal tumors when injected intraperitoneally into athymic nude mice. (biomedcentral.com)
The aim of the present study was to assess the effects of sprouty homolog 2 (SPRY2) gene regulation by miR-21 on the occurrence, development and tumor metastasis in multiple myeloma (MM). The miR‑21 expression lentiviral vector (LV)‑anti‑miR‑21 and a liposome transfection method were used to screen MM cell lines with stable silent SPRY2. (spandidos-publications.com)
Real‑time quantitative PCR and western blot analysis showed that in the MM cell lines with high endogenous miR‑21 expression (RPMI8226 and KM3), SPRY2 expression was significantly lower. (spandidos-publications.com)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)

MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)
To reveal the roles of CDC2/Cyclin B1 in human malignant gliomas, CDC2 in glioma cell line, it was down-regulated by retrovirus vectors expressing short hairpin RNAs (shRNAs) ex vivo and in vivo. (biomedcentral.com)
BALB/c-nu/nu mice were used for the in vivo proliferation experiment. (biomedcentral.com)

We propose that Per8p is a component of the molecular machinery that controls the proliferation of this organelle. (rupress.org)
Gliomas are the most common and aggressive primary brain tumors for which unfortunately no effective treatment modalities exist despite advances in molecular biology as the knowledge base to unravel the extremely complex molecular mechanisms of tumorigenesis is limited. (biomedcentral.com)
The Organoid group, previously Clevers group, studies the molecular mechanisms of tissue development and cancer of various organs using organoids made from adult Lgr5 stem cells. (hubrecht.eu)
In APC-deficient colon carcinoma cells, we demonstrated that ß-catenin accumulates and is constitutively complexed with the TCF family member TCF4, providing a molecular explanation for the initiation of colon cancer (5). (hubrecht.eu)
The observation of this intercellular communication and that MM cells modify the microenvironment is highly suggestive that one of the first biologically relevant changes necessary for progression of MGUS or SMM to MM is for a member(s) of the clone to evolve and acquire the molecular changes needed to reprogram its immediate microenvironment into a supportive, growth-promoting microenvironment. (oncotarget.com)
Molecular Biology of the Channel Catfish Gonadotropin Receptors: 2. (biolreprod.org)
T cells have been recently identified by using key molecular markers such as CD4 and CD8 ( 4 , 5 ). (aai.org)
Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
Studies have shown that abnormal proliferation is an important part of the development of liver cancer [ 3 ].Therefore, actively exploring the molecular mechanisms that affect the proliferation of HCC can not only help to understand its occurrence and development, but also lead to the discovery of key targets. (biomedcentral.com)
We conducted our research via a combination of silico analysis and molecular biology techniques to investigate the biological function of METTL7A in the progression of cancer. (bvsalud.org)
This study integrates a couple of melanoma single cell and transcriptome sequencing datasets and performs a series of silico analyses as nicely as validation of molecular biology techniques. (bvsalud.org)

Upon immobilization sAxl advertised cell migration and induced the phosphorylation of Axl and phosphatidylinositol 3-kinase. (exposed-skin-care.net)
Experimental autoimmune encephalomyelitis (EAE), which serves as an animal model of MS, is also induced by the activation of myelin reactive CD4 + T cells and their subsequent migration in the CNS, where they mediate inflammatory responses, resulting in demyelination and neurodegeneration. (jci.org)
We found that 4HPR impairs DU145 and PC3 prostate cancer cells migration and invasion by down-regulating FAK and AKT activation and by enhancing β-catenin degradation, causing the downregulation of target genes like cyclin D1, survivin and VEGF. (biomedcentral.com)
We found that 4HPR-induced inhibition of PC cell migration and invasion correlates with decreased FAK and AKT phosphorylation, activation of the glycogen synthase kinase 3β(GSK3β) and β-catenin destabilization. (biomedcentral.com)
Knockdown of RPL5 significantly inhibited the proliferation and migration of HCT16 and RKO cells, and arrested the cell cycle in G0/G1 phase. (biomedcentral.com)
Moreover, TBHQ could partially reverse the inhibitory effect of siRPL5 on the proliferation and migration of colon cancer cells. (biomedcentral.com)
Collectively, RPL5 promoted colon cell proliferation and migration, at least in part, by activating the MAPK/ERK signaling pathway. (biomedcentral.com)
RPL5 promoted colon cell proliferation and migration, at least in part, by activating the MAPK/ERK signaling pathway, which may serve as a novel therapeutic target for cancers in which MAPK/ERK signaling is a dominant feature. (biomedcentral.com)
Here, we demonstrate that RPL5 promotes COAD cell proliferation and migration via the MAPK/ERK signaling pathway. (biomedcentral.com)
A scratch test/wound healing assay was used to detect the cell migration ability. (spandidos-publications.com)
Cell function assays showed that METTL7A knockdown promoted proliferation, invasion, migration and clone formation of melanoma cells. (bvsalud.org)

The diverse repertoire of T-cell receptors (TCR) plays a key role in the adaptive immune response to infections. (elifesciences.org)
Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)
Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)
Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)
In B cells, this class of receptors has been shown to be expressed in distinct patterns associated with development and activation [ 11 ]. (nature.com)
Two other Wnt target genes, RNF43 and ZNRF3, encode stem cell-specific E3 ligases that downregulate Wnt receptors in a negative feedback loop (35). (hubrecht.eu)
The fugu B7 molecules bound to activated T cells, indicating that putative B7 receptors were expressed upon T cells. (aai.org)
These observations indicate that fugu B7s regulate T cell responses via receptors upon T cells. (aai.org)
In addition, orphan receptors similar to mammalian costimulatory receptors such as CD28 upon the T cell have been reported in fish species ( 6 , 7 ). (aai.org)
The GnRH secreted from the hypothalamus activates specific receptors on the pituitary gland to stimulate the synthesis and secretion of FSH and LH from gonadotropic cells of the pituitary gland. (scielo.br)

PC cells usually remain confined in the organ, while a small proportion of carcinomas acquire the ability to metastasize and approximately 80% of patients who have died of advanced hormone refractory PC have clinical evidence of bone metastasis. (biomedcentral.com)
As a consequence, 4HPR led to the regulation of genes controlling cell proliferation, angiogenesis and metastasis. (biomedcentral.com)
Taken together these data suggest that versican regulates the development of peritoneal metastasis originating from cells and spheroids. (biomedcentral.com)
Background: Cancer stem cells (CSCs) have been characterized to be responsible for multidrug resistance, metastasis, recurrence, and immunosuppressive in head and neck squamous cell carcinoma (HNSCC). (bvsalud.org)

Using mice with selective deletion of STAT1 in T cells (STAT1 CD4-Cre ), we identified a potentially novel mechanism by which STAT1 regulates neuroinflammation independently of Foxp3 + Tregs. (jci.org)
Protein phosphorylation regulates actomyosin-driven vesicle movement in cell extracts isolated from the green algae, Chara corallina. (bio.net)
We revealed the mechanism by which NCAPG regulates the proliferation of HCC: NCAPG inhibits PTEN through its interaction with CKII, and then activates the PI3K-AKT pathway to promote the proliferation of HCC. (biomedcentral.com)

An MTT assay was used to assess cell proliferation. (spandidos-publications.com)
A Transwell assay was used to detect the cell invasion ability. (spandidos-publications.com)
The effect of NCAPG on the proliferation of HCC cell lines was evaluated using an EdU incorporation assay, a Cell Counting Kit-8 assay and Fluorescence in situ hybridization (FISH). (biomedcentral.com)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
Chemotherapy may be used, particularly in preparation for bone marrow or hematopoietic stem cell transplantation. (medscape.com)

CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck. (biolegend.com)

KLRB1C/NKR-P1C activates NK-cell cytotoxicity, while KLRB1B/NKR-P1B functions as an inhibitory receptor. (bdbiosciences.com)
Our cell-based assays are strategically designed based on various factors such as disease states, proliferation, and cytotoxicity. (marinbio.com)

Abnormalities in cell cycle regulation are reported to be strongly associated with tumorigenesis and progression of tumors. (biomedcentral.com)
They are known to be involved in the regulation of NK and T cell responses, mostly by homotypic interactions except for SLAMF2 (CD48) and SLAMF4 that interact with each other [ 10 ]. (nature.com)
Since angiogenesis and metastatic spread are strictly related, in this study we analyzed the regulation of multiple signaling pathways responsible for cancer cell invasion. (biomedcentral.com)

Effector CD4 + T cell subsets have been defined based on their secretion of cytokines, as well as based on transcription factors and cytokines required for their development and maintenance. (jci.org)

Escape from immune control must be important in the natural course of B-cell lymphomas, especially for those with activation of NF-κB. (springer.com)
The pre-clinical LMP1/CD40-expressing transgenic mouse model is characterized by B-cell specific CD40 signaling responsible for NF-κB continuous activation with a spleen monoclonal B-cell tumor after 1 year in 60% of cases. (springer.com)
NF-κB constitutive activation is found either in aggressive diffuse large B-cell lymphomas (DLBCL) with an activated phenotype (ABC-DLBCL), or in indolent B-cell lymphomas such as chronic lymphocytic leukemia, Waldenström Macroglobulinemia, marginal zone B-cell lymphomas (MZL) [ 4 ]. (springer.com)
Here, we wanted to explore the putative interest of PD-L1 immune therapy against B-cell lymphoma with NF-κB activation. (springer.com)
These data provide evidence that anti-OX40 prior to surgery is safe and can increase activation and proliferation of CD4+ and CD8+ T cells in blood and tumor. (bepress.com)
At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
TIGIT is a negative regulator of NK and T cell activation. (biolegend.com)
Expansion of Th1 cells is then promoted by IL-12-induced STAT4 activation. (jci.org)
In mammals, professional APCs induce adaptive immunity via the activation of T cells. (aai.org)
Despite its importance, the evolutionary process of the T cell activation system via APCs remains poorly understood because of the lack of an effective model organism in lower vertebrates that could allow one to manipulate leukocytes and to use suitable markers for the identification of APCs. (aai.org)
Thus, the evolutionary history of the T cell activation system via APCs remains unclear. (aai.org)
N2a-Rα cells exhibited constant activation of the exogenous AhR. (biomedcentral.com)

These data point to 4HPR as a negative regulator of AKT phosphorylation, effectively targeting the β-catenin pathway and inducing a relatively benign phenotype in prostate cancer cells, limiting neoangiogenesis and cell invasion. (biomedcentral.com)

This non-migratory phenotype was similarly produced in both cell lines by stable silencing of β-catenin. (biomedcentral.com)

constitutively released by murine main cells such as dendritic and transformed cell lines. (exposed-skin-care.net)
The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. (biolegend.com)
Dendritic cells (DCs), 4 monocytes/macrophages, and B cells have been identified as major professional APCs ( 1 , 2 ). (aai.org)

Here, we explored the mechanism by which NCAPG enhances the proliferation of HCC. (biomedcentral.com)

CMI) , cellular i. immune responses that are initiated by an antigen-presenting cell interacting with and mediated by T lymphocytes ( e.g., graft rejection, delayed-type hypersensitivity). (theodora.com)
T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)
The resulting diverse naïve repertoire contains T-cell clones that recognize epitopes of yet unseen pathogens, and can participate in the immune response to infection or vaccination. (elifesciences.org)
As reviewed recently, various publications have indicated that disruption of immune checkpoints is also a critical step in B-cell non-Hodgkin's Lymphomas (NHL) [ 3 ]. (springer.com)
Spleen from mice were collected and immune cells were filtered through a sterile nylon membrane. (springer.com)
Are B Cells unique to an individual's immune system? (stackexchange.com)
The advantage in using T-cells is that these leukocytes can orchestrate an immune response, mobilizing the several, diverse elements of the immune system to respond to a specific antigen. (stackexchange.com)
In fish, a variety of specific immune responses such as acute allograft rejection indicate that T cells are involved in these immune activities in a similar manner to those of mammals ( 3 ). (aai.org)
Macrophages are the most abundant white blood cells in the immune microenvironment and have a high degree of heterogeneity. (frontiersin.org)
The presence of immune cells in UCB that are not directed against maternal foreign Ags is remarkable and may explain the reported clinical observation of improved HSCT outcome with younger sibling donors. (ashpublications.org)
Correlation with the level of immune cell infiltration was evaluated by multiple algorithms. (bvsalud.org)

The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)

Therefore, our study suggests that the STAT1 pathway can be manipulated to limit autoreactive T cells during autoimmunity directed against the CNS. (jci.org)

For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 10 6 cells in 100 µl volume. (biolegend.com)
Here we question whether solely transmaternal cell flow may immunize UCB. (ashpublications.org)
The immunization is presumably caused by transmaternal cell flow of male microchimerism present in the mother. (ashpublications.org)
3 In this study, we hypothesize that younger siblings are better donors as they might have come into contact with nonshared Ags of their older siblings through transmaternal cell flow. (ashpublications.org)
Although the effect of birth order could not be confirmed, 4 the possibility of transmaternal cell flow has been speculated about for a long time. (ashpublications.org)
A consequence of the latter cell flow was recently demonstrated in a clinical study in cord blood transplantation. (ashpublications.org)
Flow cytometry was used for analysis of cell cycle. (spandidos-publications.com)
All patients can evolve into trisomy 8+ MDS [12], cells, since acquiring adequate samples diagnosed with MDS have a reduced in which 8+ appears to confer a favour- for flow cytometric analysis renders the life expectancy compared with age- able prognosis [13]. (who.int)

In mammals, physiological Wnt signaling is intimately involved with the biology of adult stem cells and self-renewing tissues (18,19). (hubrecht.eu)
These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (32, 47, 53). (hubrecht.eu)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Processes involved in later stage disease, like development of androgen independence as a consequence of androgen depletion therapy, neoangiogenesis and homing of metastatic cells in lymphatic or bone tissues are generally undetectable at early stages. (biomedcentral.com)
27 Jun, 2007 06:08 pm Stem cells have the potential to become all the cells and tissues in the human body. (scitizen.com)
Successful colonization of the abdomen by EOC cells and spheroids to a large extent depends on their ability to attach to mesothelial surfaces of the peritoneal organs and tissues. (biomedcentral.com)
Methods: We depicted the landscape of HNSCC transcriptome profile by single-cell RNA-sequencing analysis of 20 HNSCC tissues from public databases, to reveal the Cell components, trajectory changes, signaling network, malignancy status and functional enrichment of CSCs within tumors. (bvsalud.org)

The cell cycle is a complex process with myriad genes involved and elaborate and complex signaling mechanisms to allow for this critical cellular process. (biomedcentral.com)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
Over the last decade, significant investigation in the MM field has demonstrated that the interaction of malignant PCs with BM stromal cells within the microenvironment is essential for survival of the PC clone [ 5 , 6 ]. (oncotarget.com)

Furthermore, PCL is feasible in a few hours in opposition to classical cloning or induced pluripotent stem cells (iPS) which all need weeks or months. (wikipedia.org)

Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells. (bepress.com)
Analyses of CD8+ TIL show increases in tumor-antigen reactive, proliferating CD103+ CD39+ cells in 25% of patients with evaluable tumor tissue (N = 4/16), all of whom remain disease-free. (bepress.com)
however, the antigen is also expressed on a rare, specialized population of T lymphocytes (NK-T cells) and some cultured monocytes. (bdbiosciences.com)
CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. (biolegend.com)
This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

Second-line chemotherapy regimens vary, depending on whether hematopoietic stem cell transplantation (HSCT) is being considered. (medscape.com)
Although treatment strategies changed from traditional chemotherapy and autologous hematopoietic stem cell transplantation to novel targeted drug therapy, the outcome was not improved ( 2 ). (spandidos-publications.com)

Here we describe the results from a phase Ib clinical trial (NCT02274155) in which 17 patients with locally advanced head and neck squamous cell carcinoma (HNSCC) received a murine anti-human OX40 agonist antibody (MEDI6469) prior to definitive surgical resection. (bepress.com)
Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes 3 . (biolegend.com)
This antibody has also been used for depletion of CD8a + cells. (biolegend.com)
This Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60. (sigmaaldrich.com)
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. (biolegend.com)
However, there are only a few of these cells for each antibody. (stackexchange.com)
F) Magnified images of (E). (E and F) Apoptotic cells were labeled with anti-cleaved Caspase-3 antibody (red). (fsu.edu)

The specificity of T-cells is determined by the T-cell receptor (TCR), a heterodimer of alpha and beta protein chains. (elifesciences.org)
The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation. (rupress.org)
A common heterophilic ligand for these RTK family members is definitely Gas6 a vitamin K-dependent protein that is widely secreted by most cells including the lungs intestine Serping1 and vascular endothelium (43). (exposed-skin-care.net)
Gas6 is the product of growth arrest-specific gene 6 which was in the beginning cloned from serum-starved fibroblasts and shares about CUDC-305 (DEBIO-0932 ) 44% sequence identity and related website organization with protein S a negative regulator of blood coagulation (48). (exposed-skin-care.net)
T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. (biolegend.com)
TotalSeq™-D antibodies are compatible with Mission Bio's Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein. (biolegend.com)
These assays not only measure the quantitative effects of drugs but are also crucial for evaluating DNA/RNA/protein/small molecules/nanoparticles in biology assays . (marinbio.com)
The ribosomal protein RPL5 is a component of the ribosome, a large ribonucleoprotein complex responsible for protein synthesis in cells. (biomedcentral.com)
DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)

In summary, this study demonstrates the significance of MV shedding and MV-mediated intercellular communication on malignant plasma cell proliferation, and identifies the role of MV-enriched CD147 in this process. (oncotarget.com)

Since the SLAMF low signature was strongly associated with an unmutated CLL immunoglobulin heavy chain (IGHV) status in large datasets, we investigated the impact of SLAMF1 and SLAMF7 on the B cell receptor (BCR) signaling axis. (nature.com)

In collaboration with Dr. Fujita at University College London, we found that Mahj-knockdown mammalian MDCK cells were also eliminated by wildtype cells through cell competition. (fsu.edu)

Here, we describe the characterization of one of these mutants, per8, and the cloning of the PER8 gene. (rupress.org)
Cloning and characterization of an active fragment of luciferase from a luminescent marine alga, Pyrocystis lunula. (bio.net)

In mammals, APCs induce adaptive immunity that in turn activates T cell responses ( 1 ). (aai.org)

The signaling mechanisms of IL-21 in different cell types have been identified. (intechopen.com)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)

Several studies shown that Axl could mediate cell adhesion and aggregation through homotypic ectodomain associations (9 23 Both CUDC-305 (DEBIO-0932 ) murine and human being Axl proteins undergo proteolytic processing to yield a soluble form of this molecule. (exposed-skin-care.net)
No immunoreactivity is seen with murine EC, EG or ES cells. (sigmaaldrich.com)
The undifferentiated murine neuroblastoma Neuro2a cell line (ATCC) was stably transfected with AhR cDNA and the established cell line was named N2a-Rα. (biomedcentral.com)

Notably PCL rejuvenates exclusively one single tissue or organ, in contrast to classical cloning PCL is therefore unable to reconstitute an entire organism. (wikipedia.org)
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P). (biolegend.com)
These cells represent the epithelial stem cells of the small intestine and colon (23), the hair follicle (24), the stomach (28) and many other tissue stem cell types. (hubrecht.eu)
MarinBio has extensive experience with cells and cell banks at both non-GMP/‑GLP and GMP/GLP levels of many tissue cell types and clones, in addition to custom development of cells, clones, or sub-clones. (marinbio.com)
8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)

Unlock the full potential of your drug discovery process with MarinBio's Cell-Based Assays Services. (marinbio.com)
Cell-based assays (bioassays) measure functional effects of drugs to establish their biological relevance and physiological importance. (marinbio.com)

B-F) Wing discs with mahj−/− (lacking GFP), wild-type (expressing GFP strongly), and mahj+/− clones (expressing GFP moderately) at 72 h (B), 96 h (C, E, and F), and 120 h (D) after clone induction. (fsu.edu)
We also proved that CKII was the key factor in the induction of proliferation by NCAPG. (biomedcentral.com)

2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Indeed, our experiments showed significantly decreased degranulation capacity of primary NK cells from CLL patients expressing low levels of SLAMF1 and SLAMF7. (nature.com)
RNA-seq results showed that transcription factors and macrophage inflammatory proteins were significantly downregulated in the DNMT3A mutant clones. (frontiersin.org)
High-risk MDS cases had a significantly higher percentage of apoptotic CD34+ cells and anti-apoptotic survivin+ cells than controls, particularly for trisomy 8 cases. (who.int)

The data demonstrate that versican is instrumental in facilitating cell and spheroid adhesion to the mesothelial cell monolayers, as its reduction with specific shRNAs led to decreased adhesion. (biomedcentral.com)

18 Sep, 2007 12:13 pm Rice University researchers have engineered musculoskeletal cartilages with human embryonic stem cells, with the hope of eventually using the neotissue. (scitizen.com)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

The key notion that exemplifies "partial" cloning from "classical" cloning is the separation of the mechanism(s) that "wipe clean" the specialization of a cell from those that "wipe-clean" the age of the cell. (wikipedia.org)
NCAPG, non-SMC subunit in the concentrate I complex, might promote the proliferation of hepatocellular carcinoma (HCC), but the mechanism is unclear. (biomedcentral.com)
As an alkylating agent, the mechanism of action of active metabolites may involve cross-linking of DNA, which may interfere with growth of normal and neoplastic cells. (medscape.com)

OX40, GITR, and 4-1BB), can enhance T cell immunity in tumor-bearing hosts. (bepress.com)
Remember first that the human body nearly always, although there are exceptions, rejects any non-genetically identical graft placed into it, and therefore one cannot prepare a general colony of B-cells to confer immunity to the population at large. (stackexchange.com)

Recently, genetic analyses in Drosophila have revealed various genes that regulate the competitive behavior of cells. (fsu.edu)

ACH transformed rabbit PBMC, as indicated by interleukin-2-independent proliferation of a transfectant culture. (wustl.edu)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Amongst the intestinal Wnt target genes (13), we found the Gpr49/Lgr5 gene to be unique in that it marks small cycling cells at crypt bottoms. (hubrecht.eu)
The Wnt target gene encoding the transcription factor Achaete scute-like 2 controls intestinal stem cell state (26). (hubrecht.eu)
Daughters of the small intestinal stem cells, the Paneth cells, serve as crypt niche cells by providing Wnt, Notch and EGF signals (33). (hubrecht.eu)

Classical cloning can rejuvenate old cells but the process demands that the old cells must artificially pass through an embryonic cell stage. (wikipedia.org)
Partial cloning affords the advantage that the old cells to be rejuvenated do not have to pass through the embryonic cell stage and are simply made younger. (wikipedia.org)
Thus the specialized functions of the adult cell are also "wiped clean" and returned to an embryonic cell type. (wikipedia.org)

Anatomy13

Organisms5

Diseases3

Chemicals and Drugs13

Analytical, Diagnostic and Therapeutic Techniques and Equipment16

Phenomena and Processes31

Disciplines and Occupations8

Technology, Industry, Agriculture1

Humanities2

Information Science3

Health Care1

Differentiation32

Apoptosis14

Vitro16

Inhibit5

Clonal7

Expression27

Vivo3

Molecular12

Migration11

Receptors11

Metastasis4

Regulates3

Assay3

Bone marrow6

Promotes1

Cytotoxicity2

Regulation3

Secretion1

Activation12

Invasion1

Phenotype1

Dendritic3

Enhances1

Immune11

Concomitant1

Pathway1

Flow8

Tissues7

Cellular2

Survival2

Pluripotent stem1

Antigen7

Hematopoietic2

Antibody7

Protein9

Intercellular communication1

Receptor1

Knockdown1

Characterization2

Activates1

Mechanisms2

Murine3

Tissue5

Assays2

Induction2

20021

Significantly3

Adhesion1

Human embryonic2

Mechanism3

Immunity2

Genes that regulate1

Transfectant1

Multipotent progenitor cells1

Intestinal3

Embryonic cell3