Proliferation by regulating hepatocellular carcinoma cells micro rna that regulates. Medical search. Frequent questions

Many of these miRNAs act as tumor suppressors and/or oncogenes, and are involved in cell activities that include development, differentiation, proliferation, apoptosis, metabolism and immunity ( 7 , 10 , 11 ). (spandidos-publications.com)
Recently, increasing evidences elucidated that miRNAs was involved in the cancer pathogenesis, including cell proliferation, metastasis, and apoptosis [ 6 ]. (hindawi.com)
Also, in gastric cancer, miR-455 inhibited human cell proliferation and invasion and promoted cell apoptosis [ 12 ]. (hindawi.com)
In addition, miR-455 inhibited cell viability, while it induced cell apoptosis in colorectal cancer [ 13 ]. (hindawi.com)
Cell apoptosis was analyzed by flow cytometry. (degruyter.com)
Moreover, TGFBR2 was a direct target of miR-128-3p, and its overexpression reversed the inhibitory effects of miR-128-3p overexpression on inflammation and apoptosis in LPS-induced HK2 cells. (degruyter.com)
miR-128-3p could inhibit apoptosis and inflammation by targeting TGFBR2 in LPS-induced HK2 cells, which might provide therapeutic strategy for the treatment of sepsis. (degruyter.com)
Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)
Induction of autophagy and apoptosis by miR-148a through the sonic hedgehog signaling pathway in hepatic stellate cells. (ajcr.us)
STAT1 is known to regulate several genes which are involved in cell growth, apoptosis, immune responses, and lipid metabolism. (thermofisher.com)
Annexin V-PE/PI and PI staining were used to analyze cell apoptosis and cell cycle distribution by flow cytometry, respectively. (biomedcentral.com)
Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)
AD is associated with abnormal proliferation, apoptosis and phenotype of VSMCs[ 4 ]. (ijpsonline.com)
Although KLF6 overexpression in human cancer cells inhibits cell proliferation, induces apoptosis, and attenuates cell migration, the effects of KLF6 on oral cancer remains poorly elucidated. (medsci.org)
KLFs regulate a wide range of cellular functions, including cell proliferation, apoptosis, differentiation, and neoplastic transformation, by binding to GC-rich promoter regions [ 1 , 2 ]. (medsci.org)
however, forced KLF6 expression in lung cancer cell lines can trigger cells to undergo apoptosis and reduce colony formation ability [ 6 ]. (medsci.org)
Then, the chemical count kit-8 cell viability assay, Annexin V-FITC apoptosis detection, EdU labeling proliferation detection, transwell and wound healing experiments were performed. (researchsquare.com)
The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. (researchsquare.com)
In this study, we constructed NFAT2 overexpressed cells (HepG2/NFAT2) based on HepG2 and investigated the effect of NFAT2 overexpression on HepG2 cells' viability, apoptosis, proliferation, invasion and migration. (researchsquare.com)
MicroRNA-543 promotes cell invasion and impedes apoptosis in pituitary adenoma via activating the Wnt/β-catenin pathway by negative regulation of Smad7. (cancerindex.org)
HP75 cells treated with overexpressed miR-543 exhibited increased cell proliferation, migration and invasion, while decreased cell apoptosis as well as expression of Cleaved caspase-3 and Cleaved caspase-8 were observed. (cancerindex.org)
Then, agomir-20b-5p and antagomir-20b-5p were transfected into MCF-7 and T47D breast cancer cells to detect cell migration, wound healing and proliferation, and lentivirus vectors silencing or overexpressing miR-20b-5p were transfected into T47D-CSCs to detect proliferation and apoptosis. (researchsquare.com)

MicroRNAs (miRNAs) are small, endogenous, non-coding RNAs, which act as key post-transcriptional regulators of target-gene expression. (spandidos-publications.com)
1,000 human miRNAs have been identified that regulate ~1/3 of the coding genes in the human genome ( 9 ). (spandidos-publications.com)
MicroRNAs (miRNAs) are regulatory small non-coding RNAs that can regulate gene expression by binding to gene elements, such as the gene promotor 5?UTR, mainly in the 3?UTR of mRNA. (molcells.org)
One miRNA targets many mRNAs, which can be regulated by many miRNAs, leading to a complex metabolic network. (molcells.org)
MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
MicroRNAs (miRNAs) are a kind of small noncoding RNAs that regulate the expression of protein through targeting the 3′UTR of messenger RNA (mRNA), resulting in mRNA degradation or suppression of translation [ 5 ]. (degruyter.com)
microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
microRNAs (miRNAs) have been emerged as a class of novel noncoding RNA regulator, and their length are approximately 22 nucleotides in length [ 4 ]. (hindawi.com)
Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). (biomedcentral.com)
A series of studies indicate that lncRNAs serve as competing endogenous RNAs (ceRNA) by sponging miRNAs, and modulate the targets of miRNAs [ 15 , 16 ]. (biomedcentral.com)
Another level of regulation may lie on microRNAs (miRNAs), which are endogenously expressed small non-coding RNAs of 18-24 nucleotides in length that regulate gene expression on the posttranscriptional level [ 14 ]. (biomedcentral.com)
microRNAs also play a crucial role in endothelial inflammation, senescence and susceptibility to atherosclerosis: endothelial inflammation is critically regulated by miRNAs such as miR-126 and miR-10a, and endothelial aging is additionally controlled by miR-217 and miR-34a [ 15 ]. (biomedcentral.com)
A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
In the case of RNA molecules that bind target RNA segments, such as microRNAs (miRNAs), the altered base pairing can change binding specificities. (biomedcentral.com)
Model based on GA and DNN for prediction of mRNA-Smad7 expression regulated by miRNAs in breast cancer. (cancerindex.org)
miRNAs regulate proteins expressions by arresting or degrading the mRNAs. (cancerindex.org)
MicroRNAs (miRNAs) have become a hot topic in cancer research, but the underlying mechanism of its involvement in cancer remains unclear. (researchsquare.com)
Recent studies, including our previous findings, have demonstrated that miRNAs play an important role in regulating biological functions [6-8]. (researchsquare.com)
Because of their unique expression profile and special functions, miRNAs have been used as molecular markers to define the direction and processes of cell differentiation by regulating one or several target genes. (researchsquare.com)
MiRNAs can also control the self-renewal or proliferation of stem cells [9]. (researchsquare.com)
Previous studies have confirmed that miRNAs play an important role in gene regulation in CD44 + CD24 -/low breast cancer stem cells (BCSCs). (researchsquare.com)
moreover, normal cell regulation can be disrupted by tumor-suppressive or oncogenic miRNAs. (scirp.org)
MicroRNAs (miRNAs) negatively regulate gene expression post-transcriptionally by binding to the 3'-untranslated region (3'-UTR) of the target mRNA sequences and have been shown to be involved in various types of cancers. (biomedcentral.com)
MicroRNAs (miRNAs), with the length of 18-22 nucleotides, have been shown to downregulate target mRNA expression post-transcriptionally by binding to the 3'-untranslated region (UTR) of the target mRNA sequences [ 9 ]. (biomedcentral.com)
DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)

Thus, the pivotal roles of miR-455 in cell viability and metastasis in HCC still needed to be explored. (hindawi.com)
Modulation of PAK4 signaling has been shown to lead to significant functional implications in a number of disease conditions, exemplified by oncogenesis, cancer cell invasion and metastasis. (wikipedia.org)
Muc1 promotes migration and lung metastasis of melanoma cells. (ajcr.us)
MicroRNA-328 is associated with (non-small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration. (genosensor.co)
Our study is the first to provide demonstrate that KLF6 functions as a tumor suppressor gene and prevents the metastasis of oral cancer cells. (medsci.org)
Therefore, besides the regulation of immune cells, NFAT2 also exerts modulatory effect of proliferation, invasion, metastasis and malignancy in breast cancer, lung cancer, melanoma and leukemia [13-15]. (researchsquare.com)
Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
The tumor microenvironment (TM) in close contact with cancer cells is highly related to tumor growth and cancer metastasis. (peerj.com)
The abnormality of those functions in the organic microenvironments promoted the metastasis of HCC and initiated the biogenesis of a secondary HCC in a PM when the PM encountered the invasion of cancer cells. (peerj.com)

Up-regulated CKS2 promotes tumor progression and predicts a poor prognosis in human colorectal cancer. (ajcr.us)
Up regulation of the long non-coding RNA NEAT1 promotes esophageal squamous cell carcinomacell progression and correlates with poor prognosis. (ajcr.us)
This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
Although microRNA-29a-3p was reported to inhibit laryngocarcinoma progression, the potential mechanisms have not been explored clearly. (hindawi.com)
MiR-29a-3p was enriched in hBMSC-derived Exo, and the Exo from miR-29a-3p mimics transfected hBMSCs could inhibit laryngocarcinoma cell malignant phenotypes in vitro and prevent tumor progression in vivo . (hindawi.com)
In conclusion, hBMSC-derived Exo with upregulated miR-29a-3p inhibited laryngocarcinoma progression via regulating PTEN, providing a potential diagnostic and therapeutic target in this disease. (hindawi.com)
Previous studies revealed that inhibition of miR-744-3p could restore PTEN expression, and then suppressed laryngeal squamous cell carcinoma progression [ 20 ], suggesting that upregulation of PTEN could contribute to cancer development. (hindawi.com)
The loss of KLF6 expression is also correlated with cancer progression, tumor recurrence, and short survival time in head and neck carcinomas [ 7 ]. (medsci.org)
EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
Additionally, miR-146a silencing or restoration of FLAP led to a reduction of HepG2 cell proliferation, cell cycle progression, migration, and invasion. (biomedcentral.com)
LncRNA AGPG has been shown to play a role in esophageal cancer by binding to and stabilizing PFKB3, which increases glycolytic flux and cell cycle progression in esophageal cancer cells. (ijbs.com)

Further, high NEK2 expression promoted proliferation, colony formation, migration and invasion of HCC cell lines. (oncotarget.com)
microRNA-506 regulates proliferation, migration and invasion in hepatocellular carcinoma by targeting F-spondin 1 (SPON1). (ajcr.us)
Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)
KLF6 overexpression attenuated the migration and invasion of oral cancer SAS cells. (medsci.org)
Furthermore, NFAT2 participates in the early growth response factor 2 (Egr2)-regulated anergic phenotype which impairs the migration and invasion of leukaemia cells [16]. (researchsquare.com)
Next, the effects of miR-637 on glioma cell migration and invasion were determined by using the transwell assay. (scirp.org)
In addition, miR-637 suppressed glioma cell migration and invasion. (scirp.org)
Consequently, we aimed to assess miR-637 expression in gliomas to determine the effects of miR-637 on glioma cell migration and invasion, as well as to explore the possible signaling pathways involved. (scirp.org)
This study showed that miR-146a has a stimulatory role in HepG2 cells and promotes HepG2 cell migration and invasion by targeting FLAP mRNA. (biomedcentral.com)

Downregulation of miR-590-5p inhibited proliferation and invasion of hepatocellular carcinoma cells (HCCs). (molcells.org)
We also showed that expression of TGF-beta RII, which has been regarded as a regulator of tumor proliferation, invasion, and migration in hepatocellular carcinoma, is regulated by miRNA-590-5p. (molcells.org)
We also found that downregulation of miR-590-5p was associated with an elevation of TGF-beta RII and inhibition of proliferation and invasion in HepG2 cells. (molcells.org)
Furthermore, overex-pression of miR-590-5p was associated with upregulation of TGF-beta RII and could promote proliferation and invasion in L02 cells. (molcells.org)
Thus, the role of TGF-beta RII in regulating proliferation and invasion of human HCCs is controlled by miR-590-5p. (molcells.org)
In other words, miR-590-5p promotes proliferation and invasion in human HCCs by directly targeting TGF-beta RII. (molcells.org)
Cell viability and invasion were measured by CCK8 and Transwell assays. (hindawi.com)
miR-455 suppressed cell proliferation in vitro and in vivo, and it inhibited the abilities of cell invasion and EMT in HCC. (hindawi.com)
RAB18 reversed partial roles of miR-455 on cell viability and invasion in HCC. (hindawi.com)
miR-455 inhibited cell viability and invasion by directly targeting the 3′-UTR of RAB18 mRNA of hepatocellular carcinoma. (hindawi.com)
However, miR-455 promoted cell invasion and migration in triple-negative breast cancer [ 14 ]. (hindawi.com)
LIN28B suppresses microRNA let-7b expression to promote CD44+/LIN28B+ human pancreatic cancer stem cell proliferation and invasion. (ajcr.us)
MicroRNA-320a inhibits proliferation and invasion of breast cancer cells by targeting RAB11A. (ajcr.us)
MiR-449a suppresses cell invasion by inhibiting MAP2K1 in non-small cell lung cancer. (ajcr.us)
The miR mimics or inhibitor was transfected into laryngocarcinoma cell lines M4E and Hep2 for the investigation of the biological functions (proliferative, invasion, migratory rates, and apoptotic rates) of this miRNA. (hindawi.com)
EIF5B promotes HCC proliferation and invasion by increasing ASAP1 expression [ 5 ]. (techscience.com)

metastatic potential of HCC cells by selectively regulating epithelial-mesenchymal transition (EMT) regulatory proteins such as N-cadherin, E-cadherin, vimentin and fibronectin. (nos-nop.org)
Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)

Finally, IGF‑1R protein, phosphoinositide 3‑kinase/Akt signaling pathway‑associated proteins and cyclin pathway‑associated proteins were differentially expressed between miR‑497‑overexpressing cells and miR-497-silenced cells. (spandidos-publications.com)
Existing studies have shown that there is crossover between Renin-Angiotensin-Aldosterone (RAAS) signaling pathway and TGF-β pathway, but little is known about whether ARB drug losartan can block Angiotensin-II (Ang-II) signaling pathway, inhibit the activation of Transforming Growth Factor- Beta (TGF-β) pathway, and regulate vascular remodeling. (ijpsonline.com)
This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. (cancer-genetics.org)
In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
Consequently, Wnt signaling pathway appears as primary target of LMWH in sensitizing A2780cis cells for cisplatin toxicity. (oncotarget.com)

In this review, we focus on the relationship between RNA m 6 A methylation and gastrointestinal cancer, especially their role, mechanism and potential clinical application as biomarkers and therapeutic targets for gastrointestinal cancer. (biomedcentral.com)
miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer. (genosensor.co)
Therefore, research on hepatocellular transformations in initiation and development of HCC and identifying effective biomarkers for novel therapeutic option are still emergency challenges. (researchsquare.com)
MicroRNAs are regulatory molecules and suggested as non-invasive biomarkers for molecular diagnostics and prognostics. (biomedcentral.com)
MicroRNAs are suggested as non-invasive biomarkers for molecular diagnostics and prognostics, and some microRNAs show promising results as therapeutic targets in human trials [ 1 - 3 ]. (biomedcentral.com)
Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. (techscience.com)

However, whether miR-497 regulates other target genes in HCC is unknown. (spandidos-publications.com)
Identification of miRNA-regulated targeting genes is important for understanding their specific biological functions [ 11 ]. (degruyter.com)
Consequently, we hypothesized that some cancer-driver genes targeted by miR-31 are up-regulated in HCC as miR-31 was down-regulated in Anisole Methoxybenzene HCC. (nos-nop.org)
Next, to verify that miR-31 particularly binds to 3UTRs of also to interfere translation of these transcripts, mutant vectors harboring random mutation sequences of miR-31 biding sites from the 3UTR of and genes had been generated, and each vector was co-transfected with miR-31 into SNU-449 and SKHep-1 cells. (nos-nop.org)
After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of many different genes. (thermofisher.com)
Our analysis revealed a number of genes previously associated with hepatocellular carcinoma and uncovered their association network in the blood. (frontiersin.org)
This correlation indicates that linked genes are likely part of a shared regulatory mechanism in the cell. (frontiersin.org)
EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. (biomedcentral.com)
Stimulating the expression of angiogenic microRNAs or genes in EPCs of low activity (such as those from patients with cardiovascular diseases) might allow the development of novel therapeutic strategies. (biomedcentral.com)
ADARs have been shown to contribute to disease pathologies by editing of glutamate receptors, editing of serotonin receptors, mutations in ADAR genes, and by other mechanisms, including recently identified regulatory roles in microRNA processing. (biomedcentral.com)
We previously identified differentially expressed microRNAs with liver-specific target genes in plasma from children with chronic hepatitis B. To further understand the biological role of these microRNAs in the pathogenesis of chronic hepatitis B, we have used the human liver cell line HepG2, with and without HBV replication, after transfection of hepatitis B virus expression vectors. (biomedcentral.com)
RT-qPCR is the preferred method for microRNA studies, and a careful normalisation strategy, verifying the optimal set of reference genes, is decisive for correctly evaluating microRNA expression levels. (biomedcentral.com)
The aim of this study was to provide valid reference genes for the human HCC-derived cell line HepG2. (biomedcentral.com)
A panel of 739 microRNAs was screened to identify the most stably expressed microRNAs, followed by a PubMed search identifying microRNAs previously used as reference genes. (biomedcentral.com)
We identified miR-24-3p, miR-151a-5p, and miR-425-5p as the most valid combination of reference genes for microRNA RT-qPCR studies in our hepatitis B virus replicating HepG2 cell model. (biomedcentral.com)
This study is to explore the biogenesis mechanism of a secondary hepatocellular carcinoma (HCC) based on the function of RNA binding proteins (RBPs)-encoding genes in the physiological microenvironment (PM). (peerj.com)
The genes encoding RBPs and the relevant genes are involved in the transformation from PM to PTM, then constructing the TM by regulating protein synthesis. (peerj.com)
However, it is still enigmatic about the exact processes in which RBPs-encoding genes and the relevant genes participate in transcriptome level during the biogenesis of secondary tumor in the activity of hepatocellular carcinoma (HCC) cells. (peerj.com)

microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
Krüppel-like factors can bind to specific DNA motifs and regulate various cellular functions, such as metabolism, cell proliferation, and differentiation. (medsci.org)
Nuclear factor of activated T cells 2 (NFAT2, also known as NFATc and NFATc1) is an important member of NFAT family which plays a vital role in T cell activation and differentiation [4]. (researchsquare.com)

Examples of PAK4-regulated cellular processes include, dynamic reorganization of actin, and microtubule fibers, anchorage-independent growth, filopodium formation, and cell motility ITGB5, cell survival embryonic development, supports stem cell-like phenotypes, and gene expression. (wikipedia.org)
STAT1 is activated to regulate gene expression in response to extracellular signaling polypeptides including cytokines, interferons, and growth factors. (thermofisher.com)
MicroRNA 429 Regulates Mucin Gene Expression and Secretion in Murine Model of Colitis. (genosensor.co)
NcRNAs play a significant role in regulating gene expression in digestive system tumors. (ijbs.com)

HCC tissues derived from chemical-induced rat liver cancer models validated that miR-31 manifestation is significantly down-regulated, and that those cell cycle- and EMT-regulatory proteins are deregulated in rat liver cancer. (nos-nop.org)
Overall, we suggest that miR-31 functions like a tumor suppressor by selectively regulating cell cycle and EMT regulatory proteins in human being hepatocarcinogenesis providing a novel target FANCG for the molecular treatment of liver malignancies. (nos-nop.org)
m 6 A is catalysed by RNA methyltransferases METTL3, METTL14 and WTAP (writers), m 6 A is removed by the demethylases FTO and ALKBH5 (erasers) and interacts with m6A-binding proteins, such as YT521-B homology (YTH) domain-containing proteins. (biomedcentral.com)
Krüppel-like factors (KLFs) are highly conserved zinc-finger proteins that regulate cellular transcription machinery [ 1 , 2 ]. (medsci.org)
In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
NcRNAs are a class of RNA molecules that do not encode proteins, and they play an important role in regulating cellular transcription and post-transcriptional processes [ 7 ]. (ijbs.com)
The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)

Hepatocellular carcinoma (HCC) has been regarded as the fifth most common cancer worldwide with a low prognosis. (hindawi.com)

The fact that and are up-regulated in HCC led us to hypothesize that normal and expressions are balanced by endogenous miR-31, which selectively regulates and mRNA translation in normal hepatic liver cells. (nos-nop.org)
Mechanistically, PTTG3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 (PARP2) by sponging miR-383, acting as a competing endogenous RNA (ceRNA). (biomedcentral.com)

MicroRNA-29b-2-5p inhibits cell proliferation by directly targeting Cbl-b in pancreatic ductal adenocarcinoma. (genosensor.co)

The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. (hindawi.com)
RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. (hindawi.com)
All the fresh tissues were snap-frozen in liquid nitrogen and stored at −80°C until RNA extraction. (hindawi.com)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
The miR-29a-3p level was decreased, and PTEN level was elevated in laryngocarcinoma tissues and the cancer cell lines. (hindawi.com)
In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated. (researchsquare.com)
Compared with adjacent tissues, the carcinoma tissues expressed less NFAT2, Egr2, FasL and more COX-2 and c-myc. (researchsquare.com)
The absence of NFAT2 and Egr2 in carcinoma tissues reminded us that NFAT2 may be a promising therapeutic target for hepatocellular carcinoma treatment. (researchsquare.com)
In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
A-to-I editing, which is catalyzed by enzymes of the adenosine deaminase acting on RNA (ADAR) family, is most prevalent in the central nervous system (CNS) but occurs in many tissues [ 1 - 3 ]. (biomedcentral.com)
Up-regulated miR-543 and downregulated Smad7 were observed in PA tissues. (cancerindex.org)
The RNAs play important roles in the pathological and physiological tissues including cancer. (peerj.com)
Herein, we aimed to demonstrate the expression of miR-146a and FLAP in human HCC tissues and liver cancer cell lines. (biomedcentral.com)
We demonstrated that miR-146a expression is overexpressed, while FLAP protein and mRNA are suppressed in hepatocellular carcinoma tissues and HepG2 cells compared to para-carcinoma tissues and HL-7702 cells. (biomedcentral.com)

This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. (nih.gov)
Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
However, another article reported that ALKBH5 promotes hepatocellular carcinogenesis in HBV-related HCC and catalyzes m6A demethylation of HBx mRNA to sustain its expression [ 23 ]. (biomedcentral.com)

The long non-coding RNA lnc-ZNF180-2 is a prognostic biomarker in patients with clear cell renal cell carcinoma. (ajcr.us)

Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells. (techscience.com)

Numbers shown below represent number of publications held in OncomiRDB database for Oncogenic and Tumor-Suppressive MicroRNAs. (cancerindex.org)
MiR-125b participates in the oncogenic role of lncRNA MALAT1 in the phenotypes including cell proliferative and invasive rates in laryngocarcinoma [ 6 ]. (hindawi.com)
Oncogenic miR-20b-5p was confirmed to promote the malignant behaviors of breast cancer cells and BCSCs. (researchsquare.com)

Therefore, the present study investigated miR‑497 expression in HCC samples and HCC‑derived cell lines using reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
Pseudogene, a subclass of long non-coding RNAs (lncRNAs), are considered as genomic loci that resemble real gene, but lost some functionality because they are lack of protein-coding ability because of disabling mutation, lack of transcription, or their inability to encode RNA [ 3 ]. (biomedcentral.com)
Further, only one study has reported that miR-637 can inhibit tumorigenesis through disruption of signal transducers and activation of transcription 3 signalings in hepatocellular carcinoma [9]. (scirp.org)

Hepatocellular carcinoma (HCC) is the most common primary cancer of the liver. (spandidos-publications.com)
We analyzed the relationship between differential expression of NEK2 and hepatocellular carcinoma (HCC) patient outcomes after liver transplants. (oncotarget.com)
These findings suggest miR-486-5p negatively regulates NEK2, which is a critical prognostic indicator of HCC patient survival after liver transplantation. (oncotarget.com)
The manifestation of miR-31 was normalized to U6 snRNA (*test) (D) The qRT-PCR analysis of miR-31 for hepatocellular carcinoma cell lines (n=7) and liver normal cell lines (n=2) (**test). (nos-nop.org)
Furthermore, we augmented cell-free RNA network analysis with single-cell RNA sequencing data which enables the contextualization of the identified network modules with cell-type specific transcriptomes from the liver. (frontiersin.org)
Instead we use an in vitro HBV-replicating liver cell model to investigate the biological role of microRNAs with a possible influence on the pathogenesis of CHB in children. (biomedcentral.com)

Thus, umbilical cord blood EPCs hold great therapeutic potential for cell therapy and vascular engineering. (biomedcentral.com)
Low molecular weight heparin (LMWH), the guideline based drug for prophylaxis and treatment of cancer-associated thrombosis, was recently shown to sensitize cisplatin resistant A2780cis human ovarian cancer cells for cisplatin cytotoxicity upon 24 h pretreatment with 50 μg × mL -1 of the LMWH tinzaparin in vitro , equivalent to a therapeutic dosage. (oncotarget.com)

In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. (nih.gov)
As demonstrated in Fig. ?Fig.2B,2B, knockdown augmented HDAC2 and CDK2 protein expressions in SNU-449 and SKHep-1 cells, whereas co-transfection of miR-31 mimics attenuated knockdown effect on the same cells. (nos-nop.org)
Liquid biopsy represents a promising alternative to invasive tissue biopsies in particular for cancer diagnosis as it enables the study of cell-free (cf) nucleic acids in the blood, which includes cfDNA and various cfRNAs (e.g., protein-coding, lncRNA, microRNA, etc. (frontiersin.org)
The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. (cancer-genetics.org)
NFAT2, as an important transcriptional promoter, regulates expression of TNF-α, myc proto-oncogene protein (c-myc), cyclooxygenase-2 (COX-2), Fas ligand (FasL) and also generates crosstalks with ERK/MAPK pathway and AKT/GSK3b signaling, which achieves its control of the cell fate [7-12]. (researchsquare.com)
Abnormal expression of 5-Lipoxygenase Activating Protein (FLAP) has been detected in many tumor cells. (biomedcentral.com)
Tonicity-responsive enhancer-binding protein (TonEBP), which is also known as nuclear factor of activated T cells 5 (NFAT5), was discovered 20 years ago as a transcriptional regulator of the cellular response to hypertonic (hyperosmotic salinity) stress in the renal medulla. (nature.com)
Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)

In addition, overexpression of miR‑497 was observed to inhibit colony formation and tumor growth in MHCC‑97H human HCC cells. (spandidos-publications.com)
The sensitivity and Ca 2+ mobilization of HepG2 cells was also inhibited by NFAT2 overexpression. (researchsquare.com)
The effect of miR-20b-5p on xenograft growth was investigated in vivo by transfection of a lentivirus-overexpression vector into T47D cells. (researchsquare.com)

Based on the findings, the inhibition of miR-543 was found to play a tumor suppressive role in PA through the down-regulation of Wnt/β-catenin pathway by negatively regulating Smad7. (cancerindex.org)

Nuclear factor of activated T cells 2 (NFAT2) has been reported to regulate the development and malignancy of few tumors. (researchsquare.com)
The current study firstly suggested that NFAT2 suppressed the aggression and malignancy of HepG2 cells through inducing the expression of Egr2. (researchsquare.com)

The binding sites of miR-128-3p and TGFBR2 were predicted by Targetscan online software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. (degruyter.com)
Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)

CCK-8 assay was performed to examine cell proliferation. (biomedcentral.com)
Using TCF/LEF luciferase promotor assay (Top/Flash) we show that resistant A2780cis cells possess a threefold higher Wnt signaling activity compared to A2780 cells. (oncotarget.com)

Analogous to DNA methylation and histone modification, RNA modification, as another epigenetic layer, plays an important role in many diseases, especially in tumours. (biomedcentral.com)
As the most common form of RNA modification, m 6 A methylation has attracted increasing research interest in recent years. (biomedcentral.com)
Although the full scope of epigenetics has not yet been determined, it is generally defined as chemical modification that mainly includes DNA and RNA methylation, histone modification, noncoding RNA modification and chromatin rearrangement. (biomedcentral.com)
In addition to DNA and histone methylation, another level of epigenetic regulation, namely, RNA methylation, has become a hot topic in biosciences over the past decade. (biomedcentral.com)
Common RNA methylation sites include 5-methylcytosine (m 5 C), 7-methylguanosine (m 7 G), m 1 G, m 2 G, m 6 G, N 1 -methyladenosine (m 1 A) and m 6 A. m 5 C modification promotes splicing and translation [ 4 ]. (biomedcentral.com)
The function of N6-methyladenosine (m6A) RNA methylation depends on a variety of methyltransferases and demethylases. (biomedcentral.com)
Dynamic regulation of RNA methylation mainly depends on ''writers'' and ''erasers'', which function as dedicated RNA methyltransferases and demethylases, respectively [ 10 ]. (biomedcentral.com)

Transcriptome-wide research reveals that m 6 A modification may affect more than 7000 mRNAs in individual transcriptomes of mammalian cells. (biomedcentral.com)
In this study, we aimed at characterizing extracellular transcriptome gene networks of hepatocellular carcinoma patients compared to healthy controls. (frontiersin.org)
A-to-I editing is a mechanism that regulates and diversifies the transcriptome, but the full biological significance of ADARs is not understood. (biomedcentral.com)
Eukaryotic RNA transcripts can undergo a range of post-transcriptional modifications, which increase the diversity of the transcriptome without requiring increases in genome size. (biomedcentral.com)

Similar to PAK1, PAK4-signaling-dependent cellular functions also regulate both physiologic and disease processes such as cancer, as PAK4 is overexpressed and/or hyperstimulated in human cancer, at-large. (wikipedia.org)
PAK4 regulates cellular processes by its scaffolding activity and/or by phosphorylation of effector substrates, which in-turn, set-up a cascades of biochemical events cumulating into a cellular phenotypic response. (wikipedia.org)
Thereby, LMWH induced sensitization by transcriptional reprogramming of A2780cis cells via not yet elucidated mechanisms that depend on cellular proteoglycans. (oncotarget.com)

The five yr Anisole Methoxybenzene survival rate was significantly decreased in patient with low level of miR-31 manifestation in the tumor cells (Log-rank = 0.0015*) (C) The qRT-PCR analysis for 9 paired HCC cells. (nos-nop.org)
In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)

Thus, A-to-I editing in both translated and untranslated regions of RNA can be biologically significant. (biomedcentral.com)

In our study, we found that the expression level of miR-590-5p is higher in the human hepatocellular carcinoma cell line HepG2 than in the normal hepatocellular cell line L02. (molcells.org)
We also studied the microRNAs that affect NEK2 expression. (oncotarget.com)
Tumor xenograft data from Balb/c nude mice demonstrated that HCC cells with high NEK2 expression formed larger tumors than those with low NEK2 expression. (oncotarget.com)
The level of miR-128-3p was decreased, and TGFBR2 expression was increased in serum samples of sepsis patients and LPS-induced HK2 cells. (degruyter.com)
Our study thus demonstrates the feasibility of performing gene co-expression network analysis from cell-free RNA data and its utility in studying hepatocellular carcinoma. (frontiersin.org)
In this study, we aimed to explore the effect of NFAT2 expression on cell fate of HepG2 cell and its potential mechanisms. (researchsquare.com)
Hence, LMWH reduces the cisplatin-induced rise in Wnt activity and TCF-4 expression in A2780cis cells, but keeps sensitive A2780 cells unaffected. (oncotarget.com)
Altered expression levels of specific microRNAs are associated with hepatitis B virus infection and hepatocellular carcinoma. (biomedcentral.com)
Even small changes in microRNA expression may therefore have implications for gene regulation in various physiological and pathophysiological states [ 1 , 6 ]. (biomedcentral.com)
The present study was conducted to identify suitable RGs for microRNA expression studies in a human HCC-derived cell line (HepG2 tet-on), with and without HBV replication, after transfection of HBV expression vectors. (biomedcentral.com)
Knockdown of miR-146a also resulted in increased FLAP expression of cancer cells. (biomedcentral.com)
Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)

Role of Dusp6 Phosphatase as a Tumor Suppressor in Non-Small Cell Lung Cancer. (cancer-genetics.org)
Based on our identified miRNA-regulated network, we discovered three drugsBX-912, daunorubicin, and midostaurinthat can be repositioned to treat lung cancer, which was not known before. (cdc.gov)

It focuses on how precision oncology has shaped advances in the impact of non-coding RNAs in epigenetics and cancer, immunotherapy and tumor biology, and the clinical significance of various therapies in a range of cancers. (biomedcentral.com)

RNA editing refers to post-transcriptional processes that alter the nucleotide sequence of an RNA transcript by insertion, deletion or nucleotide conversion. (biomedcentral.com)

Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)

DUSP6/MKP3 is a dual-specific phosphatase that regulates extracellular regulated kinase ERK1/2 and ERK5 activity, with an increasingly recognized role as tumor suppressor. (cancer-genetics.org)

Hepatocellular carcinoma (HCC) one of the most common digestive system tumors, threatens the tens of thousands of people with high morbidity and mortality world widely. (biomedcentral.com)

Recently, an increasing number of studies have revealed that microRNA-497 (miR-497) levels are decreased in tumors, and that it functions as a tumor suppressor in a number of types of human cancer, including colorectal, gastric, cervical and breast cancers, adrenocortical carcinoma and melanoma ( 12 - 20 ). (spandidos-publications.com)

m 6 A is the most common among various RNA modifications [ 9 ] and has critical roles in cancer pathogenesis. (biomedcentral.com)

The role of miR‑497 in regulating the proliferation of HCC‑derived cells was also investigated in vitro and in vivo. (spandidos-publications.com)

Firstly, the pcDNA3.1-NFAT2 plasmid was transfected into HepG2 cells to construct NFAT2 overexpressed HepG2 cells. (researchsquare.com)

Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-fluorouracil (5-FU). (techscience.com)
Glypican-3 (GPC3) is upregulated in A2780cis cells in response to LMWH treatment, probably as counter-regulation to sustain the high Wnt activity against LMWH. (oncotarget.com)

The samples were analyzed using the technology of RNA-seq and bioinformatics. (peerj.com)

Three-dimensional assessment of bystander effects of mesenchymal stem cells carrying a cytosine deaminase gene on glioma cells. (ajcr.us)

ALKBH5 is downregulated and has antitumor effects in HCC cells. (biomedcentral.com)

To date, many studies have focused on WGCNA analysis of bulk or single-cell RNA (scRNA) sequencing data from tissue samples. (frontiersin.org)

The exosomes (Exo) from human bone marrow mesenchymal stem cells (hBMSCs) after the transfection of miR mimics/inhibitor/si-PTEN were isolated and used to stimulate M4E and Hep2 cells. (hindawi.com)

Selective inhibition of EGFR downstream signaling reverses the irradiation-enhanced migration of HNSCC cells. (ajcr.us)
Inhibition of Let7c microRNA is neuroprotective in a rat intracerebral hemorrhage model. (genosensor.co)

In addition, functional studies have shown that TonEBP is involved in the pathogenesis of rheumatoid arthritis, atherosclerosis, diabetic nephropathy, acute kidney injury, hyperlipidaemia and insulin resistance, autoimmune diseases (including type 1 diabetes mellitus and multiple sclerosis), salt-sensitive hypertension and hepatocellular carcinoma. (nature.com)

Anatomy11

Organisms10

Diseases49

Chemicals and Drugs94

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes47

Disciplines and Occupations1

Information Science5

Health Care11

Apoptosis23

MiRNAs29

Metastasis9

Progression12

Migration and invasion9

Invasion16

Epithelial-mesenchyma2

Signaling pathway5

Biomarkers6

Genes18

Differentiation3

Gene expression4

Proteins7

Prognosis1

Endogenous2

Inhibits cell proliferation1

Tissues15

MRNA4

Clear cell renal cell carc1

Abstract1

Oncogenic3

Transcription3

Liver6

Therapeutic2

Protein8

Overexpression3

Negatively1

Malignancy2

Reporter assay2

Assay2

Methylation7

Transcriptome4

Cellular3

Survival2

Biologically significant1

Expression12

Lung cancer2

Oncology1

Post-transcriptional processes1

Invasiveness1

Extracellular1

Digestive system tumors1

Tumors1

Roles1

Vitro1

HepG21

Regulation2

Bioinformatics1

Glioma cells1

ALKBH51

Tissue samples1

Transfection1

Inhibition2

Pathogenesis1