Proliferation and differentiation of mammalian neural stem cells artificial adult mammalian cells. Medical search. Frequent questions

In these regions, neural stem cells (NSCs) and neural progenitor cells (NPCs) have been reported to proliferate and produce postmitotic neurons. (jneurology.com)
In the last decade, cortical adult neurogenesis and its neural stem cells (NSCs), which are self-renewing and can generate neurons, astrocytes and oligodendrocytes, and neural progenitor cells (NPCs), which have a tendency to differentiate into certain types of neurons, have been found in the cerebral cortex of adult mammals 3 . (jneurology.com)
Adult p107-null mice had elevated numbers of proliferating progenitor cells in their lateral ventricles. (rupress.org)
In adult mouse hippocampus, Tbr2 protein and Tbr2-GFP (green fluorescent protein) transgene expression were specifically localized to intermediate-stage progenitor cells (IPCs), a type of transit amplifying cells. (jneurosci.org)
suggesting that progenitor cells in the embryonic and adult brain may share similar molecular phenotypes. (jneurosci.org)
Through cell survival-based cDNA expression screens in neural progenitor cells, we identify a genetic variant of AGS Atp5g1 that confers cell resilience to metabolic stress. (elifesciences.org)
Therefore, the therapeutic use of appropriate populations derived from stem and progenitor cells has been considered for cell-based therapies for neurodegenerative diseases, primarily Parkinson's disease, and brain injuries. (ehu.eus)
Adult stem cells can be used to accelerate bone or tendon healing , and they can induce cartilage progenitor cells to produce a better matrix and repair cartilage damage . (thefutureofthings.com)

Moreover, certain drugs, such as antidepressants, can accelerate production of neurons from cortical NPCs, and increased neurons from cortical NPCs have the inhibitory effect on neuronal cell death, which is induced by global ischemia 4 . (jneurology.com)
This work evaluated the effect of ex situ management on neurogenesis (cell proliferation in the dorsal and medial ventricular zones, neuronal integration in the dorsomedial and medial cortices), ovary cell proliferation, body size (mass and length) and self-righting ability. (frontiersin.org)
At the molecular level, we found that Cul3 controls neuronal migration by tightly regulating the amount of Plastin3 (Pls3), a previously unrecognized player of neural migration. (nature.com)
Remarkably, the same TF cascade was found to be linked to stages of neuronal lineage progression in adult SGZ. (jneurosci.org)
Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. (novusbio.com)
Upon an electrochemical switch, growth factor presentation was reversed, which initiated cellular differentiation along the neuronal lineages. (5dok.org)
Methods: We used organotypic cultures of human retina and preparations of dissociated cells to test the hypothesis that cell fusion between human MG and adult stem cells can induce neuronal regeneration in human systems. (regenerativemedicine.net)
Initially, two‐dimensional neural cultures presented an innovative landmark for investigating human neuronal and, more recently, glial biology, as well as for modeling brain neurodevelopmental and neurodegenerative diseases. (ehu.eus)
I use a multidisciplinary methodology that integrates advanced single cell RNAseq, imaging, multi-parameter flow cytometric isolation of cellular populations and transplantation assays to elucidate the cellular and genetic changes associated with neuronal degeneration process in young and old colonies. (stanford.edu)
Here, we characterized neuronal death in B. schlosseri, a marine colonial tunicate that shares substantial genomic homology with mammals and has a life history in which controlled neurodegeneration happens simultaneously in the brains of adult zooids during a cyclical phase named takeover. (stanford.edu)
Using an ultrastructural and transcriptomic approach, we described neuronal death forms in adult zooids before and during the takeover phase while comparing adult zooids in takeover with their buds where brains are refining their structure. (stanford.edu)

Moreover, pathological processes, such as neuroinflammation, stroke or epilepsy, are able to induce proliferation and differentiation of NSCs and NPCs. (jneurology.com)
Th9 cells can promote the secretion of CCL20 from epithelial cells and induce a powerful antitumor Compact disc8+ CTL impact by marketing CCL20/CCR6-reliant recruitment of dendritic cells into tumors. (tech-strategy.org)
CYB5A trapped our principal focused not only be induce of lack of knowledge concerning its sub cellular area and down regulation in nuclear membrane of HCC in contrast to fibrotic liver and HepG2 cell lines, but also mainly because of S nitrosylation of this protein. (kspinhibitors.com)
We hypothesize that ovarian cancer cells will induce human ovarian TVM expression. (nih.gov)

In particular, the advent of human embryonic stem cells followed by reprograming technologies for generation of induced pluripotent stem cells have instigated studies into modeling human brain development and disease by providing a means to simulate a human tissue otherwise completely or largely inaccessible to researchers. (ehu.eus)
In rodents, and even in some preliminary trials in humans, human embryonic stem cells have been shown to bridge gaps in spinal cord injuries , allowing restoration of motor functions. (thefutureofthings.com)
A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)

precursor cells. (immune-source.com)
Recently, we developed a hematopoietic stem cell-based approach to tackle this issue, in which we can genetically alter nucleated hematopoietic precursor cells and differentiate them ex-vivo to mature erythrocytes that can be infected by P. falciparum. (stanford.edu)
Enriched monolayer precursor cell cultures from micro-dissected adult mouse dentate gyrus yield functional granule cell-like neurons. (mdc-berlin.de)

MATERIALS AND METHODS: IE-6 and Caco-2 cells were used to evaluate the effect of Lico A on apoptosis, proliferation, and migration of IECs. (bvsalud.org)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)
Apoptosis is a caspase regulated cell death present in all metazoans defined by a conserved set of morphological features. (biomedcentral.com)
A well-described function of apoptosis is the removal of excessive cells during development and homeostasis. (biomedcentral.com)
In contrast to the apoptotic function of cell elimination, this new role of apoptosis is not well understood but seems caspase-dependent. (biomedcentral.com)
To deepen our understanding of apoptotic functions, it is necessary to work on a biological model with a predictable apoptosis pattern affecting cell fate and/or behaviour. (biomedcentral.com)
Apoptosis is also implicated in homoeostasis by fine-tuning the balance between cell death and proliferation like in gonad size control in rodents [ 11 ]. (biomedcentral.com)
While the function of cell elimination by apoptosis is highly documented, apoptosis seems to have a broader morphogenetic function that goes beyond cells elimination. (biomedcentral.com)
Apoptotic-induced proliferation was reported in several organisms, such as in the cnidarian Hydra , where apoptosis promotes proliferation of adjacent cells through emission of Wnt3 signalling in a caspase-dependant manner during head regeneration [ 14 ]. (biomedcentral.com)
Evaluating the capacity of caspases to affect gene expression and influence fate and/or behaviour of cells adjacent to apoptosis is the first fundamental step to better characterise this new function in animals. (biomedcentral.com)
Western Blotting was used to determine the level of expression of CDC2 protein as measure to quantify down regulation of CDC2 expression along with use of flow cytometry to investigate effect of shRNAs on cell cycles and detection of apoptosis. (biomedcentral.com)
Retroviral vectors expressing short hairpin RNAs (shRNAs) against CDC2 caused efficient deletion of CDC2, cellular G2/M arrest concluding in apoptosis and inhibition of proliferation in human glioma cells U251 and SHG-44 cell lines ex vivo. (biomedcentral.com)

In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107 −/− brains that exhibited enhanced capacity for self-renewal. (rupress.org)
We discuss what is known about the distinct metabolic states captured in vitro by the 2-cell-like, naïve, blastocyst-like, formative, and primed states of pluripotency. (conditionmed.org)
2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Weighed against Tc1 cells, Tc9 cells possess a weaker cytolytic capability in vitro, however in OT-I/ B16-OVA and pmel-1/B16 melanoma versions, Tc9 cells possess a consistent and solid antitumor impact [39, 40]. (tech-strategy.org)
Using biologically relevant elastic substrates to study cell function in vitro has proven beneficial, as the in vivo microenvironment usually is much softer than rigid plastic dishes. (5dok.org)
Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)

These results suggest that Tbr2+ IPCs play a major role in the regulation of adult hippocampal neurogenesis, and that a similar transcriptional program controls neurogenesis in adult SGZ as in embryonic cerebral cortex. (jneurosci.org)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
While regarded by many top scientists as the Holy Grail of medicine, others consider embryonic stem-cell research sacrilegious. (thefutureofthings.com)
In the future, embryonic stem cells may be able to restore sight to millions of people. (thefutureofthings.com)
As the embryonic cells divide and the daughter cells differentiate, they become increasingly specific. (thefutureofthings.com)
The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
The cells of the ICM are no longer omnipotent, because they no longer share the fate of the trophoblast, and they have committed themselves to an embryonic fate with the ability to become any cell in the body (but not the trophoblast). (thefutureofthings.com)
These adult stem cells are considered multipotent, having the ability to differentiate into different cell types, albeit with a more limited repertoire than embryonic stem cells. (thefutureofthings.com)
The rest of this article will deal with embryonic stem (ES) cells and the future they hold for modern medicine. (thefutureofthings.com)

It is widely accepted that new neurons are generated throughout life, which is called adult neurogenesis, in limited regions of the adult mammalian brain, such as the hippocampal dentate gyrus and subventricular zone. (jneurology.com)
After that, using the DNA synthesis marker tritiated thymidine, Altman rediscovered the addition of new neurons in the cerebral cortex of adult rats 6 . (jneurology.com)
However, in mice, in vivo cell fusion between MG and adult stem cells forms hybrids that can partially regenerate ablated neurons. (regenerativemedicine.net)
A particular field encouraged by the foundation is stem-cell research, with the great hope that it will result in the ability to get cells to differentiate into neurons and support cells to bridge the gap of a spinal cord injury. (thefutureofthings.com)
We found that neurons in the brain and cells of the vascular network both express multiple genes for voltage gated Na+ and Ca2+ ion channels homologous (based on sequence) to mammalian ion channel genes. (stanford.edu)

We explore the recently described metabolic surge event that occurs as pluripotency is lost and stem cells commit to differentiate. (conditionmed.org)
Prior research show that naive T cells Azelaic acid can differentiate into Th9 cells in the current presence of IL-4 and TGF- [21]. (tech-strategy.org)
Tc9 cells can differentiate from Azelaic acid Compact disc8+ T cells within Azelaic acid a Th9 cell-induced environment [37]. (tech-strategy.org)
Next, we showed that the resulting hybrids can differentiate and acquire a proto-neural electrophysiology profile when the Wnt/beta-catenin pathway is activated in the adult stem cells prior fusion. (regenerativemedicine.net)
Interpretation: We show fusion between human MG and adult stem cells, and demonstrate that the resulting hybrid cells can differentiate towards neural fate in human model systems. (regenerativemedicine.net)
At the top of the list comes the zygote-a fertilized egg, which of course has the ability to divide and differentiate into all cell types in the body and create a new organism. (thefutureofthings.com)
The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)

When caspase activity was impaired, genes known to be involved in metamorphosis were downregulated along with other implicated in cell migration and survival molecular pathways. (biomedcentral.com)
Unlike IGF1 receptor (IGF1R) and insulin receptor (IR), IGF2R does not have intrinsic tyrosine kinase activity but can recruit G proteins, and even though these G-protein-activated pathways are not well characterized in the brain, PKC and phospholipase C are involved in IGF2 actions in the brain [ 6 ] and other cell types [ 7 ], respectively. (scientificarchives.com)
Pleiotropic IL-9 can control the function of T cells, B cells, mast airway and cells epithelium cells by activating the STAT1, STAT3 and STAT5 signaling pathways, which get excited about the development of tumor illnesses, hypersensitive illnesses, inflammatory, and autoimmune illnesses [8]. (tech-strategy.org)
These compounds generally penetrate cell membranes, act on specific target proteins in cells, regulate intracellular signaling pathways, and cause some changes in cell phenotype. (chemscene.com)
However, central neural pathways in naive mammals that recognize sex of other individuals remain poorly characterized. (stanford.edu)
In the case of the latter, these advances mark a watershed because scientists can now focus on discrete neural pathways in an effort to understand how the brain encodes these fundamental social behaviors. (stanford.edu)
These results were confirmed by transcriptomic analyses that highlighted the specific genes involved in these cell death pathways. (stanford.edu)

However, it remains largely unknown what factors can regulate adult neurogenesis in the cerebral cortex. (jneurology.com)
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)
Currently, little is known about molecular mechanisms that regulate proliferation and differentiation in the SGZ. (jneurosci.org)
Cholesterol can regulate the creation of IL-9 by Tc9 cells adversely, impacting the antitumor immunity [42] thus. (tech-strategy.org)

They have been alternately referred to as "pluripotent" growth factors and as "promiscuous" growth factors due to their multiple actions on multiple cell types. (wikipedia.org)
In 2006, Kazutoshi Takahashi and Shinya Yamanaka reprogrammed mice fibroblast cells, which can produce only other fibroblast cells, to become pluripotent stem cells, which have the capacity to produce many different types of cells. (asu.edu)
They called the pluripotent stem cells that they produced induced pluripotent stem cells (iPSCs) because they had induced the adult cells, called differentiated cells, to become pluripotent stem cells through genetic manipulation. (asu.edu)
The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
On the cover: Human induced pluripotent stem cell-derived neuroepithelial-like stem cells stained for actin (green) and DNA (blue). (5dok.org)
These cells are considered pluripotent . (thefutureofthings.com)

Furthermore, we found that Pls3 cell-autonomously regulates cell migration by regulating actin cytoskeleton organization, and its levels are inversely proportional to neural migration speed. (nature.com)
This signaling pathway is a highly conserved cell-to-cell communication system that regulates cell fate in development and disease. (5dok.org)
Eph/ephrin signaling is a cell-to-cell communication pathway, which regulates cell migration and proliferation. (5dok.org)

Adult neurogenesis in these regions is influenced by various factors. (jneurology.com)
Recently, adult neurogenesis in the cerebral cortex is becoming clear gradually, and cortical NSCs and NPCs are identified in a few mammals. (jneurology.com)
This review focuses on the effects of regulating factors on cortical adult neurogenesis, such as brain damages, aging and certain drugs, and we discuss implications of cortical adult neurogenesis for brain diseases and damages. (jneurology.com)
There are many reports that adult neurogenesis occurs in the subventricular zone (SVZ) and hippocampal dentate gyrus (HDG) of mammalian brains since the 1960s 1,2 . (jneurology.com)
Adult neurogenesis in the SVZ and HDG has been reported to be regulated by some regulatory factors, such as stress, environmental enrichment, exercise, certain drugs, aging, and so on. (jneurology.com)
On the other hand, it remains controversial whether adult neurogenesis occurs in other regions of the central nervous system (CNS). (jneurology.com)
In this review, I summarize recent studies of cortical adult neurogenesis. (jneurology.com)
In particular, I focus on regulating factors of cortical adult neurogenesis. (jneurology.com)
Historically, the discovery of cortical adult neurogenesis is old, and identifying generation of new cells in the adult cerebral cortex was first reported in 1912 5 . (jneurology.com)
Neurogenesis in the adult hippocampus is a highly regulated process that originates from multipotent progenitors in the subgranular zone (SGZ). (jneurosci.org)
Adult hippocampal neurogenesis is a constitutive but highly regulated process that occurs in a neurogenic niche, the subgranular zone (SGZ) of the dentate gyrus (DG). (jneurosci.org)
The functional significance of adult neurogenesis is currently not well understood. (jneurosci.org)

Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. (sdbonline.org)
Previously, we have shown that the tail regression step during metamorphosis, characterized by a predictable polarized apoptotic wave, ensures elimination of most tail cells and controls primordial germ cells survival and migration. (biomedcentral.com)
We propose these results as a confirmation that apoptotic cells can control surrounding cells fate and as a reference database to explore novel apoptotic functions in animals, including those related to migration and differentiation. (biomedcentral.com)
Accumulating evidence in many metazoans suggest that apoptotic cells can emit caspase-dependent signals to their neighbours, modulating their fate (survival/death, differentiation or proliferation) or behaviour (migration) [ 12 , 13 ]. (biomedcentral.com)
In particular, we found that Cul3 controls the abundance of Plastin 3 (Pls3), a novel player of neural cell migration, whose amount is inversely proportional to neural cell migration speed. (nature.com)
Finally, we found that CRISPR-mediated activation of Cul3 transcription fully rescues neural cell migration defects. (nature.com)
Our results show that we can tune cell stiffness and migration by regulating Notch activity and matrix stiffness. (5dok.org)
Molecular motors lie at the heart of biological processes from DNA replication to cell migration. (nih.gov)

This simplified approach towards understanding the essence of the mechanisms, underlying the processes determining life and death of a cell has undoubtedly provided scientists with enormous amount of knowledge. (intechopen.com)
Although hypoxic or ischemia tolerance in AGS involves physiological adaptations, little is known about the critical cellular mechanisms underlying intrinsic AGS cell resilience to metabolic stress. (elifesciences.org)
While much is known about biochemical signaling, many of the mechanisms that drive cell outcome in response to biophysical influences remain to be uncovered. (5dok.org)
The cell cycle is a complex process with myriad genes involved and elaborate and complex signaling mechanisms to allow for this critical cellular process. (biomedcentral.com)
Similar mechanisms may control ordinary and will cer stem cell properties. (kspinhibitors.com)
In this review, we discuss recent advances in our understanding of the molecular and neural network mechanisms underlying these behaviors in mice. (stanford.edu)

In this ongoing work, we examined the impact of thioridazine on LCSCs world development, chemoresistance, cell cycle, and in vivo prohibitory function. (immune-source.com)
Retroviral vectors expressing short hairpin RNAs (shRNAs) targeted against CDC2 were designed and transducted into human glioma cell line ex vivo in order to downregulate the expression of CDC2. (biomedcentral.com)
Downregulation of CDC2 could potentialy inhibit human gliomas cells growth ex vivo and in vivo. (biomedcentral.com)
To reveal the roles of CDC2/Cyclin B1 in human malignant gliomas, CDC2 in glioma cell line, it was down-regulated by retrovirus vectors expressing short hairpin RNAs (shRNAs) ex vivo and in vivo. (biomedcentral.com)
One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
We therefore propose (3) to isolate ovarian tumor stem cells and grow them in vivo using the ESC ovarian cancer model. (nih.gov)

Despite this, IGF2 is the most abundantly expressed IGF in the central nervous system, where it is highly synthesized in the choroid plexus and meninges in adult rat brains [ 4 ]. (scientificarchives.com)

Nevertheless, it negatively affects the ontogenesis of male gonads and brain areas homologous to the mammalian hippocampus, as well as body size and fitness. (frontiersin.org)

The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)

Notably, the Tbr2+ IPCs formed cellular clusters, the average size of which (Tbr2+ cells per cluster) likewise more than doubled in runners. (jneurosci.org)
The idea that cellular survival and growth could be maintained outside the body was recognized as possible almost hundred years ago when the German zoologist Wilhelm Roux described a successful experiment where he cultured chick neural crest in warm saline for a few days [ 1 ]. (intechopen.com)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
This protein is found in a cellular compartment called the mitochondria, which is responsible for supplying energy to the rest of the cell and therefore plays an important role in metabolic processes. (elifesciences.org)
They therefore represent a more realistic cellular environment for modeling the cell biology and pathology of the nervous system. (ehu.eus)
Multiple research groups have developed artificial mitochondrial transfer/transplantation (AMT/T) methods that transfer healthy mitochondria into damaged cells and recover cellular function. (biomedcentral.com)

A paper recently published by eLife on forebrain cortical synaptic plasticity reports that retinoic acid (RA) alters synaptopodin-dependent metaplasticity in mouse dentate granule cells (Lenz et al. (preprints.org)

4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
Stem cell technologies have opened up new avenues in the study of human biology and disease. (ehu.eus)
My expertise is in the areas of regeneration, evolution, the nervous system and cell biology. (stanford.edu)
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)

Ghini F, Rubolino C, Climent M, Simeone I, Marzi MJ, Nicassio F. Endogenous transcripts control miRNA levels and activity in mammalian cells by target-directed miRNA degradation. (eurekaselect.com)
Ectopic expression in mouse cells and CRISPR/Cas9 base editing of endogenous AGS loci revealed causal roles of one AGS-specific amino acid substitution in mediating cytoprotection by AGS ATP5G1. (elifesciences.org)
2021). However, the results reported here and those reported by others have not shown that endogenous RA is normally required for synaptic plasticity (or any other nongenomic effect) as there are no reports of genetic loss-of-function studies that remove endogenous RA in adult brain. (preprints.org)
In addition, the entire vascular network undergoes slow rhythmic contractions that appear to arise from processes endogenous to vascular epithelial cells. (stanford.edu)

Modulation of tight junction structure in blood-brain barrier endothelial cells. (worthington-biochem.com)
Usage: Mouse endothelial cells (MS1) and MS1 cells transduced to express full-length human TEM1 (MS1-TEM1) were cultured in 96-well plates to 30% confluence and then incubated for 96h in the presence of 10-fold serially diluted Streptavidin-ZAP, scFv78, or scFv78-ZAP starting from 40nM down to 0.04nM. (atsbio.com)
This study presents an in-silico model replicating a microfluidic assay where endothelial cells sprout into a biomimetic extracellular matrix, specifically, a large-pore, low-concentration fibrin-based porous hydrogel, influenced by chemotactic factors. (ibecbarcelona.eu)

Here we have investigated biophysical regulation of cell function. (5dok.org)
Abnormalities in cell cycle regulation are reported to be strongly associated with tumorigenesis and progression of tumors. (biomedcentral.com)
Collective regulation of cell motility using an accurate density-sensing system. (bge-lab.fr)

Effect confirmation in SH-SY5Y cells occurred after ATM depletion and osmotic stress better than nutrient / oxidative stress, not after ATM kinase inhibition or DNA stressor bleomycin. (preprints.org)

RACK1 was expressed transiently in the skeletal muscle of post-natal mice, being abundant in the early phase of muscle growth and almost disappearing in adult mature fibers. (sdbonline.org)
The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)
found that when this variant form of ATP5G1 was introduced into the cells of mice, their mitochondria was better at coping with stress conditions, such as low oxygen, low temperature and poisoning. (elifesciences.org)
CCR6 insufficiency might bring about lack of the antitumor aftereffect of Th9 cells in mice [16, 31]. (tech-strategy.org)

A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. (worthington-biochem.com)
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. (worthington-biochem.com)
Primary Cell Culture of Live Neurosurgically Resected Aged Adult Human Brain Cells and Single Cell Transcriptomics. (worthington-biochem.com)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
After that, tumor control cells had been reported in different types of malignancies, including lung tumor, gastric tumor, human brain cancers, liver organ cancers, and digestive tract cancers [7C11]. (immune-source.com)
One project in the lab focuses on the communication among cells of the neurovascular unit, a collection of cells that make up the blood-brain barrier (BBB), a unique characteristic of blood vessels in the brain. (dcu.ie)
In particular, due to the limited regenerative capacity of the adult nervous system, the therapeutic potential of stem cells has opened up new perspectives for development of treatment strategies for brain repair after injury or disease. (ehu.eus)
Validation experiments with stressors were performed in human neuroblastoma cells, where ATM localized only to cytoplasm, similar to brain. (preprints.org)
In the clinic, the intrinsic infil tration of single glioma cells into brain parenchyma ren ders these cancers resistant to your Inhibitors,Modulators,Libraries existing treatment of surgical removal in mixture with radiation, chemo and immuno therapies. (kspinhibitors.com)
Multiple Forms of Neural Cell Death in the Cyclical Brain Degeneration of A Colonial Chordate. (stanford.edu)
Moreover, the presence of tubulovesicular structures in the brain medulla alongside the over-expression of prion disease genes in late cycle suggested a cell-to-cell, prion-like propagation recalling the conformational disorders typical of some human neurodegenerative diseases. (stanford.edu)
Neural activity increase as the brain matures and declines thereafter. (stanford.edu)

Drawing upon a history of using tissue interactions to understand differentiation, Kollar and Baird designed their experiments to understand how differentiated structures become specified. (asu.edu)
International bi-monthly journal of cell signaling, tissue protection, and translational research. (conditionmed.org)
AQP4 is generally expressed in astrocytic endfeet where it facilitates detoxification of neural tissue through the glymphatic system. (dcu.ie)
I have recently shown that synovial tissue poly-T-cells can predict RA-patient stratification, correlate with disease progression in RA and PsA and importantly, are present at the joints of at risk-individuals prior to development of clinical-inflammation. (dcu.ie)
In addition to their ability to supply cells at the turnover rate of their respective tissues, they can be stimulated to repair injured tissue caused by liver damage, skin abrasions and blood loss. (thefutureofthings.com)
I use a marine colonial tunicate, Botryllus schlosseri, characterized by having robust regenerative capabilities and an assayable and frequent (weekly) CNS (Central nervous system) tissue regeneration and loss throughout adult life. (stanford.edu)

Cell Viability Assay The NCI-H1299 cells and 95-Deb cells were seeded into 96-well dishes at 5000 cells/well. (immune-source.com)
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. (novusbio.com)

In the latest years, people discovered that there is certainly a little subpopulation of cells in tumors, which play a essential function in the resistance of cancer to radiotherapy and chemotherapy. (immune-source.com)
cells could initiate tumors in rodents. (immune-source.com)
Current research show that Th9 cells enjoy an essential antitumor role generally in most solid tumors [29]. (tech-strategy.org)
T cell homing to tumors detected by 3D-coordinated positron emission tomography and magnetic resonance imaging. (mdc-berlin.de)

To establish protocols for the safe and efficient differentiation of healthy cells for therapies, we must develop a better understanding of the dynamic continuum of metabolic states that span pluripotency and differentiation, and how to influence them. (conditionmed.org)

Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)
Collectively, this study provides the first evidence that transient levels of the evolutionarily conserved factor RACK1 are critical for adult SC activation and proper skeletal muscle regeneration, favoring the efficient progression of SC from a committed to a fully differentiated state. (sdbonline.org)

Recent studies have shown an unexpected signalling property of apoptotic cells, affecting cell fate and/or behaviour of neighbouring cells. (biomedcentral.com)

through targeting lung cancer stem cells. (immune-source.com)
Sphere Formation The lung cancer stem cells were accumulated by sphere formation. (immune-source.com)

Human FGF18 is involved in cell development and morphogenesis in various tissues including cartilage. (wikipedia.org)
The ability of our body to regenerate some of its tissues is largely owed to the reserves of adult stem cells. (thefutureofthings.com)

Azelaic acid Moreover, IL-1 also promotes the differentiation of Th9 cells as well as the secretion of IL-21 and IL-9. (tech-strategy.org)
Time-lapse videography of living cells captures spectacular intracellular mitochondrial movement, and this movement promotes mitochondrial connections to form a dynamic mitochondrial network [ 3 ]. (biomedcentral.com)

Thereafter, using a combination of autoradiography and electron microscopy, Kaplan demonstrated that new cells containing tritiated thymidine are stellate cells that have an axonal hillock, initial segment, and synapses on their dendrites and cell bodies in the cerebral cortex of adult rats 7 . (jneurology.com)

Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)

AQP4 operates in complex with the potassium channel Kir4.1, and their cell surface expression is mediated through the dystrophin-associated protein complex (DAPC) and the extracellular matrix. (dcu.ie)
Cells sense and respond to the mechanics of their substrate - be it the extracellular matrix, neighboring cells or artificial matrix in cell culture. (5dok.org)
Distinct functions of macrophage-derived and cancer cell-derived cathepsin Z combine to promote tumor malignancy via interactions with the extracellular matrix. (uni-freiburg.de)

J Cell Biol (2004) 166 (6): 853-863. (rupress.org)

IL-9 make a difference the success of tumor cells [15] straight, or indirectly take part in tumor immunity by activating mast cells and recruiting dendritic cells (DCs) into tumor sites [16, 17]. (tech-strategy.org)

These findings provide the first evidence of a protein variant that is responsible for protecting cells during the metabolic stress conditions caused by hibernation. (elifesciences.org)
Findings: We first found that cell fusion occurs between MG and adult stem cells, in organotypic cultures of human retina as well as in cell cultures. (regenerativemedicine.net)
Based on these findings, the researchers proposed the bold idea of directly transplanting entire mitochondria, rather than drugs or whole cells, into lesions to explore the rescue effects of exogenous mitochondria. (biomedcentral.com)

Examining the genetic code of these resilient cells revealed that Arctic ground squirrels may have a variant form of a protein called ATP5G1. (elifesciences.org)
Additional study is needed to find out whether or not NO manufacturing induces post translational modifications of protein and modulates protein function in HCC cell. (kspinhibitors.com)

We propose an opportunity to target the cancer cell/microenvironment interface instead of the Notch pathway itself in the development of cancer therapies. (5dok.org)

Our results identify a naturally occurring variant of ATP5G1 from a mammalian hibernator that critically contributes to intrinsic cytoprotection against metabolic stress. (elifesciences.org)
In the field of stem cells, cumulative evidence has shown that mitochondrial transfer contributes to stem cell-triggered repair of damaged cells [ 9 ]. (biomedcentral.com)

Takahashi and Yamanaka also experimented with human cell cultures in 2007. (asu.edu)
Dynamic quantitative analysis of adherent cell cultures by means of lens-free video microscopy. (bge-lab.fr)
Imaging of dense cell cultures by multiwavelength lens-free video microscopy. (bge-lab.fr)
Lensfree diffractive tomography for the imaging of 3D cell cultures. (bge-lab.fr)

It was found that GSOs reversed GLU-induced reductions in cell viability and the LDH release rate, and rescued ROS accumulation. (sdbonline.org)
The cells were treated with different dosage of thioridazine and cell viability was assessed 2 days later or cells were treated with 10? (immune-source.com)
BC-Ppy scaffolds improved cell viability andattachment, promoting a desirable cardiomyoblast morphology. (ibecbarcelona.eu)

MeCP2 interacts with HP1 and modulates its heterochromatin association during myogenic differentiation. (mdc-berlin.de)

Identifying these changes can help us understand how diseases such as epilepsy affect these cells and may represent viable therapeutic targets. (dcu.ie)

In addition to intracellular mitochondrial movement, intercellular mitochondrial transfer between mammalian cells has recently been discovered. (biomedcentral.com)
Intercellular mitochondrial transfer can be regarded as an extension of intracellular mitochondrial movement or intercellular communication, which undoubtedly increases the mtDNA content of the recipient cells and restores the respiration and survival of the recipient cells. (biomedcentral.com)

Our results suggest that cell fusion-mediated therapy is a potential regenerative approach for treating human retinal dystrophies. (regenerativemedicine.net)
Since their discovery, stem cells have ushered in a new era in regenerative medicine. (ehu.eus)

100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined. (novusbio.com)

Finally, apoptotic cells modulate death or survival balance, as was observed in the Drosophila imaginal disc, by producing the Tie ligand Pvf1, that induces death resistance of neighbouring cells [ 17 ]. (biomedcentral.com)

These populations of cells have control cell properties. (immune-source.com)

We performed differential transcriptomic analysis between control metamorphosing larvae and larvae treated with the pan-caspase inhibitor Z-VAD-fmk in order to explore the transcriptional control of apoptotic cells on neighbouring cells that survive and migrate. (biomedcentral.com)

they are involved in a wide variety of processes, most notably as crucial elements for normal development in animal cells. (wikipedia.org)
These EVs can mediate signalling to cells throughout the body, affecting major cell processes. (dcu.ie)

Anatomy59

Organisms19

Diseases6

Chemicals and Drugs69

Analytical, Diagnostic and Therapeutic Techniques and Equipment40

Phenomena and Processes63

Disciplines and Occupations3

Information Science3

Progenitor8

Neuronal11

Induce4

Human embryonic stem3

Precursor3

Apoptosis12

Vitro9

Embryonic9

Neurons5

Differentiate7

Pathways7

Regulate4

Pluripotent6

Regulates3

Neurogenesis12

Migration8

Mechanisms6

Vivo6

Brains1

Homologous1

Germ1

Cellular6

Dentate1

Biology4

Endogenous4

Endothelial Cells3

Regulation3

Depletion1

Mice4

Brain13

Tissue6

Assay2

Tumors4

Pluripotency1

Regeneration2

Fate1

Cancer Stem2

Tissues2

Promotes2

Cerebral1

Cardiac1

Extracellular matrix3

Biol1

Tumor cells1

Findings3

Protein2

Pathway1

Contributes2

Cultures4

Viability3

Modulates1

Epilepsy1

Intracellular2

Regenerative2

Sequence1

Survival1

Populations1

Transcriptional1

Processes2