OsteosarcomaPrognosisBone NeoplasmsOsteosarcoma, JuxtacorticalFemoral NeoplasmsCell Line, TumorTumor Markers, BiologicalSurvival RateGene Expression Regulation, NeoplasticSurvival AnalysisRetrospective StudiesLung NeoplasmsLeg BonesTreatment OutcomeOsteoblastomaNeoplasm MetastasisTumor Cells, CulturedFollow-Up StudiesImmunohistochemistryHumerusNeoplasm Recurrence, LocalNeoplasm StagingKaplan-Meier EstimateSarcoma, EwingDoxorubicinAntineoplastic AgentsDisease-Free SurvivalMethotrexateChondrosarcomaDog DiseasesCell ProliferationOsteoblastsNeoplasm InvasivenessCombined Modality TherapyApoptosisTime FactorsAntineoplastic Combined Chemotherapy ProtocolsCisplatinRNA, MessengerIfosfamideAmputationMice, NudeNeoadjuvant TherapyReverse Transcriptase Polymerase Chain ReactionChemotherapy, AdjuvantDisease ProgressionGene Expression ProfilingFatal Outcome