• EpiMOGRIFY-predicted factors are able to maintain astrocytes and cardiomyocytes in vitro in chemically defined media and promote the generation of astrocytes and cardiomyocytes from neural progenitors and embryonic stem cells, respectively. (technologynetworks.com)
  • While adult stem cells have yet failed to convincingly regenerate myocardial tissue, human embryonic stem cells (hESCs) have proven to be a potential and unlimited source for cardiomyocyte regeneration. (ca.gov)
  • The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
  • Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)
  • Laminin-221 (LN221) was identified as the most likely expressed cardiac laminin and the authors show that this protein promotes differentiation of pluripotent human embryonic stem cells (hESCs) toward cardiomyocyte lineage and downregulates pluripotency and teratoma-associated genes. (biolamina.com)
  • In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
  • Additionally, PSCs - both embryonic stem cells and induced pluripotent stem cells - provide insights into the mechanisms involved in the differentiation and specification of heart cells. (ucla.edu)
  • Many directed differentiation protocols and methods rely on mimicking animal embryonic development by providing cells with stage-specific stimuli, including growth factors and small molecules, to modulate cell signaling pathway activity4. (thetechnoant.info)
  • the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)
  • Furthermore, we conduct a cross-species prediction of cardiomyocyte subtypes from in vitro-derived human induced pluripotent stem cells and unexpectedly uncover a predominance of left ventricular (LV) identity that we confirmed by an LV-specific TBX5 lineage tracing system. (stanford.edu)
  • Here, we address the question of mitochondrial adaptation by examining the differentiation of human pluripotent stem cells to cardiac progenitors and further to differentiated mesodermal derivatives, including functional cardiomyocytes. (uib.no)
  • Thus, our work highlights similarities in mitochondrial remodeling during the transition from pluripotent to multipotent state in ectodermal and mesodermal lineages, while at the same time demonstrating cell-lineage-specific adaptations upon further differentiation. (uib.no)
  • By demonstrating the generation of cardiomyocytes from murine pluripotent stem cells, their concept shows promise as a regenerative therapy. (shinshu-u.ac.jp)
  • We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)
  • However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
  • METHODS AND RESULTS: Human cardiomyocytes derived from control-induced pluripotent stem cells (iPSC-CMs) were shown to express MPO, with MPO levels being increased in iPSC-CMs generated from two HCM patients harbouring sarcomeric mutations in the MYBPC3 and MYH7 genes. (bvsalud.org)
  • During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
  • Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
  • Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
  • 1. Development of a ML/AI pipeline for the prediction of subtypes and differentiation stage of developing mouse cardiac cells from mesoderm to birth and from in vitro differentiation of human induced pluripotent stem cells (hiPSCs). (stanford.edu)
  • Written by Rebecca Quelch and Stefan Przyborski from Durham University (UK), this poster describes the isolation of pluripotent stem cells, their maintenance in culture, differentiation, and the generation and potential uses of organoids. (tocris.com)
  • Abstract Background Cardiac differentiation of human-induced pluripotent stem (hiPS) cells consistently produces a mixed population of cardiomyocytes and non-cardiac cell types, even when using well-characterized protocols. (figshare.com)
  • Mature cardiomyocytes have passed the stage of self-renewal and may pose problems due to lack of proper integration. (ca.gov)
  • We have started pre-clinical studies of transplanting cardiovascular progenitors (at different stages of development) and mature cardiomyocytes in animal models. (ca.gov)
  • Furthermore, differentiation of endogenous progenitors to mature cardiomyocytes is rarely reported. (ox.ac.uk)
  • Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiation. (stanford.edu)
  • Here, we describe the development of a TBX5/MYL2 lineage tracing reporter system that allows for the identification of FHF- progenitors and their descendants including left ventricular cardiomyocytes. (stanford.edu)
  • Together, our work provides the scientific community with a powerful new genetic lineage tracing approach as well as a single cell transcriptomic atlas of hiPSCs undergoing cardiac differentiation. (stanford.edu)
  • Hence, upon differentiation of hESCs towards cardiac lineage, a distinct fluorescent color is expressed sequentially at each stage of cardiovascular development. (ca.gov)
  • an anti-nucleolin DNA aptamer, iSN04, can determine a cell's lineage during differentiation. (shinshu-u.ac.jp)
  • Ultimately, terminal cell differentiation commits to the cardiomyocyte lineage. (shinshu-u.ac.jp)
  • Pioneer transcription factors play key roles in programming the epigenome and facilitating recruitment of additional regulatory factors during successive cell lineage specification and differentiation steps. (ox.ac.uk)
  • Here we show that Isl1 acts as a pioneer factor driving cardiomyocyte lineage commitment by shaping the chromatin landscape of cardiac progenitor cells. (ox.ac.uk)
  • Mechanistically, we show that Isl1 binds to compacted chromatin and works in concert with the Brg1-Baf60c-based SWI/SNF complex to promote permissive cardiac lineage-specific alterations in the chromatin landscape not only of genes with critical functions in cardiac progenitor cells, but also of cardiomyocyte structural genes that are highly expressed when Isl1 itself is no longer present. (ox.ac.uk)
  • 2011 Cre/loxP lineage tracing demonstrated that approximately half of the single ventricular chamber and entire OFT is derived via late differentiation and accretion of SHF progenitors following heart tube formation Glucosamine sulfate (Zhou et al. (globaltechbiz.com)
  • 2005). Recent studies suggest that these heart fields contain both unipotent and multipotent mesodermal progenitors that give rise to the diverse lineage types within the heart (Kattman et al. (baxkyardgardener.com)
  • 2006). Cardiovascular progenitors sequentially express the cardiac transcription factors (and, in response to cues from the microenvironment, undergo lineage diversification and differentiation (Laugwitz et al. (baxkyardgardener.com)
  • VE-CADHERIN is expressed transiently in early ISL1 + cardiovascular progenitor cells and facilitates cardiac differentiation. (nih.gov)
  • The overall objective of this grant is to develop new technologies that can facilitate generation of cardiovascular progenitors that upon transplantation are capable to integrate into the host tissue and function as part of the normal myocardium with no adverse effect. (ca.gov)
  • Cardiovascular progenitors, on the other hand, could adapt to the microenvironment for optimal integration into the host tissue and reside there for the lifetime of the patient. (ca.gov)
  • Myocardial infarction is the most prevalent of cardiovascular diseases and pharmacological interventions do not lead to restoration of the lost cardiomyocytes. (ox.ac.uk)
  • Cardiovascular disease remains the number one global cause of death and presents as multiple phenotypes in which the interplay between cardiomyocytes and cardiac fibroblasts (CFs) has become increasingly highlighted. (bvsalud.org)
  • A highly reproducible, chemically defined, xeno-free laminin-based differentiation protocol to generate stem cell-derived cardiovascular progenitors (CVPs) was reported in 2019 (Yap, 2019). (biolamina.com)
  • Of particular interest to Dr. Ardehali is the intrinsic signaling that trigger cardiac regeneration early in life, molecular events that regulate developmental decisions instructing cardiac progenitor cells to adopt a specific cell fate, and delivery approaches of cardiovascular progenitors into an injured heart. (ucla.edu)
  • He and his team recently identified several novel surface markers that can highly enrich for early cardiovascular progenitors. (ucla.edu)
  • When delivered into functioning human hearts that have been transplanted into animal models, the cardiovascular progenitors integrated structurally and functionally into the host myocardium. (ucla.edu)
  • and, using novel in vitro and in vivo models to identify pure populations of cardiovascular progenitors capable of engraftment into the host myocardium. (ucla.edu)
  • Myocardial infarction (MI) is one of the clusters of numerous other cardiovascular diseases and occurs due to the blockage of the coronary artery delivering blood (ischemia) to the ventricle with consequent oxygen shortage to the contractile cells (cardiomyocytes) [ 1 ] . (encyclopedia.pub)
  • Cardiac progenitors Th surviving in the heart buy 349085-38-7 crescent are shaped through the first cardiovascular field (FHF) situated in the anterior lateral dish mesoderm (LPM). (baxkyardgardener.com)
  • As the embryo builds up, FHF progenitors fuse on the midline to create the primitive cardiovascular tube, which begins to beat and, as a consequence, blood begins to circulate (DeRuiter et al. (baxkyardgardener.com)
  • A metabolic switch from glucose to fatty acid oxidation occurs during cardiac development and cardiomyocyte maturation, however in vitro differentiation protocols do not consider the lack of fatty acids in cell culture media. (ox.ac.uk)
  • In this review, we compare the in vivo development of cardiomyocytes to the in vitro differentiation of hPSC into CM with focus on electrophysiology, structure and contractility. (bvsalud.org)
  • Furthermore, it is unknown whether endogenous progenitors/stromal cells in the adult human heart could be chemically induced to generate functional atrial or ventricular heart muscle to treat adult heart diseases. (biomedcentral.com)
  • In contrast to recent findings in neuronal differentiation, we found that mitochondrial content decreases continuously during mesoderm differentiation, despite increased mitochondrial activity and higher levels of ATP-linked respiration. (uib.no)
  • Our results improve the understanding of how mitochondrial remodeling and the metabolism interact during mesoderm differentiation and show that it is erroneous to assume that increased OXPHOS activity during differentiation requires a simultaneous expansion of mitochondrial content. (uib.no)
  • is expressed in tissues that form the pharyngeal system - including pharyngeal surface ectoderm pharyngeal endoderm and pharyngeal mesoderm which contains SHF progenitors (Chapman et al. (globaltechbiz.com)
  • Second heart field (SHF) progenitors residing within the pharyngeal mesoderm (Diogo et al. (baxkyardgardener.com)
  • Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
  • Addition of a PPARα agonist during differentiation increased both glucose and fatty acid oxidation and expression of cardiac genes. (ox.ac.uk)
  • In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
  • We thus wondered how cells grow spatially during differentiation, so we labeled cells by expression of marker genes and found that cells expressing the same marker tended to occur in patches. (figshare.com)
  • For example, engineering hPSCs to increase or reduce expression of a specific gene would provide a useful way to decode the genes role in complex cell signaling networks, as well as its function in stem cell differentiation. (thetechnoant.info)
  • "Ours is the first report to a DNA aptamer that allows cardiomyocytes to develop from iPSCs," explains Dr. Takaya when asked about the significance of the work. (shinshu-u.ac.jp)
  • With the use of cardiac muscle-specific laminin-211, human iPSCs were effectively differentiated into cardiomyocytes using small molecules (Minami, 2012). (biolamina.com)
  • iPSCs were efficiently expanded and differentiated to cardiomyocytes on laminin-521 (Kuroda, 2015). (biolamina.com)
  • Analogous to bone marrow-derived progenitor cells, CPC exhibit receptor systems that are responsive to hematopoietic growth factors and similar systems may be expressed on cardiomyocytes. (baselstemcells.ch)
  • We can isolate and culture cardiomyocytes and cardiac fibroblasts (see Table 2). (gcure.nl)
  • These cells can be treated with different agents to stimulate cardiomyocyte hypertrophy in vitro or induce collagen synthesis in cardiac fibroblasts. (gcure.nl)
  • AIMS: Hypertrophic cardiomyopathy (HCM) is characterized by cardiomyocyte hypertrophy and disarray, and myocardial stiffness due to interstitial fibrosis, which result in impaired left ventricular filling and diastolic dysfunction. (bvsalud.org)
  • Finally, we found that MPO protein was expressed in healthy adult murine and human cardiomyocytes, and MPO levels were increased in diseased hearts with left ventricular hypertrophy. (bvsalud.org)
  • Laminin-521 supports increased proliferation during expansion and superior differentiation with myotube hypertrophy, larger myotubes and higher amounts of nuclei per myotube. (biolamina.com)
  • In this so-called "niche", a variety of cytokines and growth factors regulate survival as well as proliferation and differentiation capacities of CPC. (baselstemcells.ch)
  • In ongoing projects, the group is currently studying how reactive oxygen species and hematopoietic growth factors including Flt3 ligand and erythropoietin regulate the function and fate of CPC and cardiomyocytes. (baselstemcells.ch)
  • Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. (stemcell.com)
  • In order to accomplish clinically viable cell therapies, new technologies are needed to facilitate a deeper understanding of how transcription factors temporally regulate stem cell differentiation. (thetechnoant.info)
  • The predictive power of EpiMOGRIFY has been validated in two ways: cell maintenance and differentiation. (technologynetworks.com)
  • In both cell maintenance and differentiation, EpiMOGRIFY defined conditions performed as well or better in all cases when compared to existing undefined conditions, significantly increasing cell growth and survival, as well as resulting in a higher differentiation efficiency. (technologynetworks.com)
  • Chemical compounds that modulate atrial and ventricular cell fate could be used to improve subtype-specific differentiation of endogenous or exogenously delivered progenitor cells in order to promote cardiac regeneration. (biomedcentral.com)
  • Thus, small molecules capable of influencing the fate decisions and differentiation programs of multipotent progenitor cells could facilitate therapeutic regeneration of lost myocardium. (biomedcentral.com)
  • These results highlight the potential benefit of progenitor cells to generate other cell types that contribute to heart regeneration. (ca.gov)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)
  • Metabolic flux analyses to assess the differentiation of adult cardiac progenitors after fatty acid supplementation. (ox.ac.uk)
  • The aim of this study was to assess the effect of this metabolic switch on control and differentiated adult cardiac progenitors, by fatty acid supplementation. (ox.ac.uk)
  • Fetal and adult CFs influence neighboring cardiomyocytes in different ways. (bvsalud.org)
  • One of the first specialized cell types to be generated from hPSC was cardiomyocytes (CM), and differentiation protocols have evolved over the years and now allow for robust and large-scale production of hPSC-CM. Still, scientists are struggling to achieve the same, mainly ventricular, phenotype of the hPSC-CM in vitro as their adult counterpart in vivo. (bvsalud.org)
  • In vitro generated cardiomyocytes are generally described as fetal-like rather than adult. (bvsalud.org)
  • Laminin-211 has also been used for the culture of adult human cardiomyocytes (Kuroda, 2015). (biolamina.com)
  • Laminin-521 also has properties that improve the isolation and expansion of adult stem cells and progenitors. (biolamina.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • To recapitulate the phenotype of an adult cardiomyocyte, differentiating progenitors need to upregulate mitochondrial glucose and fatty acid oxidation. (ox.ac.uk)
  • We compared this data with existing murine and 3D cardiac organoid scRNA-seq data and confirmed the dominance of left ventricular cardiomyocytes (>90%) in our hiPSC-derived progeny. (stanford.edu)
  • Collagen IV promoted proliferation of CDCs whereas fibronectin primed cells for differentiation towards a cardiac phenotype. (ox.ac.uk)
  • instead, factors specifying the various cell types likely act during a window that begins shortly after the seeding of hiPS cells for differentiation. (figshare.com)
  • Written by Kirsty E. Clarke, Victoria B. Christie, Andy Whiting and Stefan A. Przyborski, this review provides an overview of the use of small molecules in the control of stem cell growth and differentiation. (tocris.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • 2011 Impairment of SHF-mediated cardiogenesis results in loss of ventricular cardiomyocytes that normally comprise the distal portion of the chamber and loss or diminution of Elastin2+ (Eln2+) smooth muscle precursor cells of the OFT. (globaltechbiz.com)
  • Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
  • Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
  • Treatment of various degenerative disorders using stem cell therapies requires directed differentiation of hPSCs into clinically applicable cell types. (thetechnoant.info)
  • Pancreatic cell difference necessitates software of stage-specific soluble inductive indicators for difference of hPSCs to defined endoderm, pancreatic progenitor, endocrine progenitor, and the differentiated cell condition7 terminally. (thetechnoant.info)
  • Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
  • The same researchers later published another article with Shinya Yamanaka as co-author, where they again used laminin-211 for cardiac differentiation of human iPSC (Hirata, 2014). (biolamina.com)
  • Dr. Joseph Wu´s lab also showed that laminin-521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes (Burridge, 2014). (biolamina.com)
  • Biolaminin 521 is an optimal matrix for chemically defined differentiation of human iPSC to cardiomyocytes. (biolamina.com)
  • results from a 25% decrease in Glucosamine sulfate cardiomyocyte numbers that occurs subseqent to heart tube stages. (globaltechbiz.com)
  • Additionally, we found that substantial cell death occurs during differentiation in a manner that suggested all cells were equally likely to survive or die, suggesting that there is no intrinsic selection bias for cells descended from particular hiPS cell progenitors. (figshare.com)
  • These findings highlight cardiomyocyte MPO as a novel therapeutic target for improving myocardial relaxation associated with HCM, a treatment strategy which can be readily investigated in the clinical setting, given that MPO inhibitors are already available for clinical testing. (bvsalud.org)
  • CPC have the capacity to differentiate into all cardiac cell lineages including cardiomyocytes and they play an important role in the maintenance of the myocardial cell homeostasis over a lifespan. (baselstemcells.ch)
  • Endocardium differentiation through Sox17 expression in endocardium precursor cells regulates heart development in mice. (nih.gov)
  • 2006). For buy 349085-38-7 example, bipotent SHF progenitors generate endocardium or easy muscle cells as well as cardiomyocytes (Lescroart et al. (baxkyardgardener.com)
  • We conclude that oxidative metabolism and cell differentiation act in partnership with increases in one driving an increase in the other. (ox.ac.uk)
  • 15 d) during cardiac differentiation in a chemically defined medium. (biolamina.com)
  • This poster summarizes some key protocols demonstrating the use of small molecules across the stem cell workflow, from reprogramming, through self-renewal, storage and differentiation to verification. (tocris.com)
  • Our Stem Cells review gives an overview of the use of small molecules in the control of stem cell growth & differentiation and somatic cell reprogramming. (tocris.com)
  • This should provide the reader with an extensive overview of the current status of human stem cell-derived cardiomyocyte phenotype and function. (bvsalud.org)
  • Here we cultured and differentiated CDCs using protocols aimed to maintain stemness or to promote differentiation, including triggering fatty acid oxidation using an agonist of peroxisome proliferator-activated receptor alpha (PPARα). (ox.ac.uk)
  • Additionally, the reader will gain insight into the underlying signaling pathways and mechanisms responsible for cardiomyocyte development. (bvsalud.org)
  • Despite recent reports suggesting conserved expression of in zebrafish SHF progenitors (Hami et al. (globaltechbiz.com)
  • Here we characterized expression in relation to cardiac progenitors and differentiated cardiomyocytes in zebrafish and analyzed null embryos for molecular and morphological evidence of SHF perturbations. (globaltechbiz.com)
  • 16 , 17 In particular, transgenic zebrafish lines that express nfsB in β cells, cardiomyocytes, or other cell types have been established, and their exposure to the metronidazole leads to the targeted loss of these cells. (cdc.gov)
  • MicroRNAs (MiRNAs) are important regulators that can strongly affect cellular functions including proliferation, differentiation, and apoptosis through post-transcriptional negative regulation of target gene expression [ 1 ]. (biomedcentral.com)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Aptamers are single-stranded pieces of DNA that bind to target proteins and are able to modulate signaling cascades during cell differentiation when a stem cell commits to a specific functional role or phenotype. (shinshu-u.ac.jp)
  • Metabolic changes were characterised in undifferentiated CDCs and during differentiation towards a cardiac phenotype. (ox.ac.uk)
  • Pioneering function of Isl1 in the epigenetic control of cardiomyocyte cell fate. (ox.ac.uk)
  • Thus, the Isl1/Brg1-Baf60c complex plays a crucial role in orchestrating proper cardiogenesis and in establishing epigenetic memory of cardiomyocyte fate commitment. (ox.ac.uk)
  • During mammalian development, the left and right ventricles arise from early populations of cardiac progenitors known as the first and second heart fields, respectively. (stanford.edu)
  • Human ES and iPS-Derived Myogenic Progenitors Restore Dystrophin and Improve Contractility upon Transplantation in Dystrophic Mice. (uh.edu)
  • Together with Otmar Pfister, a clinical cardiologist and director of the division Heart Failure and Transplantation, they are studying the regulation and role of heart-resident cardiac progenitor cells (CPC) in this remodeling process. (baselstemcells.ch)
  • Differentiation was assessed using flow cytometry and qPCR and substrate metabolism was quantified using radiolabelled substrates. (ox.ac.uk)
  • Using an Isl1 hypomorphic mouse line which shows congenital heart defects, genome-wide profiling of Isl1 binding together with RNA- and ATAC-sequencing of cardiac progenitor cells and their derivatives, we uncover a regulatory network downstream of Isl1 that orchestrates cardiogenesis. (ox.ac.uk)
  • Given the considerable interest in using stem cells for modeling and treating disease, it is essential to understand what regulates self-renewal and differentiation. (uib.no)
  • We uncovered two mechanisms of nucleolin interference with iSN04 at play whereby early treatment inhibits cardiomyogenesis, while treatment at a later stage enhances the generation of cardiac progenitors. (shinshu-u.ac.jp)
  • The platform models the epigenetic state of the cell to predict the switches important for cell identity, cell maintenance, directed differentiation and cell conversion. (technologynetworks.com)