Progenitor cells or myeloid cells. Medical search. Frequent questions

Open-Label Phase II Prospective, Randomized, Controlled Study of Romyelocel-L Myeloid Progenitor Cells to Reduce Infection During Induction Chemotherapy for Acute Myeloid Leukemia. (uchicago.edu)
Azacitidine fails to eradicate leukemic stem/progenitor cell populations in patients with acute myeloid leukemia and myelodysplasia. (ox.ac.uk)
Epigenetic therapies demonstrate significant clinical activity in acute myeloid leukemia (AML) and myelodysplasia (MDS) and constitute an important new class of therapeutic agents. (ox.ac.uk)
Assessment of distribution of CD34 epitope classes in fresh and cryopreserved peripheral blood progenitor cells and acute myeloid leukemic blasts. (unimore.it)
CD34 epitope expression was also analyzed in thawed CD34+ blasts obtained from 14 patients with acute myeloid leukemia (AML), who were analyzed using a larger number (#17) of CD34 epitope class I, II, and III reactive MoAbs. (unimore.it)
Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. (nih.gov)
The groups study present evidence of myeloid neoplasms derived from patients with acute leukemias which encompass a spectrum of PDC differentiation which result in outcomes that do not fit into the categories of MPD MN or BPDCN. (news-medical.net)
Five of these patients presented with acute myeloid leukemia, one patient suffered from unclassified MN and the final patient presented with a mixed phenotype (MPAL). (news-medical.net)
The group concluded by stating that acute leukemia with PDC differentiation and typical cases of acute myeloid leukemia, NOS, or acute myelomonocytic leukemia are indistinguishable. (news-medical.net)
Abnormalities in this process lead to a group of diseases known as myeloid malignancies, which include acute myeloid leukaemia-in which the bone marrow produces abnormal white blood cells-and myelodysplastic syndromes, which are caused by too few mature blood cells being produced. (elifesciences.org)
The origin and evolution of mutations in acute myeloid leukemia. (smw.ch)
Clonal architecture of secondary acute myeloid leukemia. (smw.ch)
Published data from our group indicate that HIF-1α regulation in airway epithelial cells (AEC) drives the acute inflammatory response following LC and GA. Metabolomic profiling and metabolic flux of Type II AEC following LC revealed marked increases in glycolytic and TCA intermediates in vivo and in vitro that were HIF-1α dependent. (jci.org)
Herein we show the expression of polySia on hematopoietic progenitor cells, and demonstrate a role for this glycan in immune response using both acute inflammatory and tumor models. (ca.gov)
Several of the critical factors involved in these decisions are mutated in acute and chronic leukemias, and their mutations tip the equilibrium in the bone marrow towards accumulation of aberrant progenitor populations. (upenn.edu)
The most comprehensive analysis yet of the genome of childhood acute myeloid leukemia (AML) found only a few mistakes in the genetic blueprint, suggesting the cancer arises from just a handful of missteps, according to new findings from St. Jude Children's Research Hospital. (scienceblog.com)
The major goals of my research are to identify how acute myeloid leukemia (AML) cells suppress the innate immune system and use this knowledge to develop new immunotherapies. (lu.se)
To characterize the function and expression patterns of the identified cell surface proteins in acute myeloid leukemia cells from patients. (lu.se)
CEBPADM) in acute myeloid leukemia (AML) with a combination of hypermorphic N-terminal mutations (CEBPANT), promoting expression of the leukemia-associated p30 isoform, and amorphic C-terminal mutations. (bvsalud.org)

The differentiation of Socs3-deficient progenitor cells is skewed toward macrophage production in response to G-CSF or IL-6, whereas wild-type progenitor cells produce mainly neutrophils. (nih.gov)
The common myeloid progenitor (CMP) and the common lymphoid progenitor (CLP) are the first branch of cell differentiation in hematopoiesis after the hemocytoblast (hematopoietic stem cell). (wikipedia.org)
These results suggest that UM171+SR1 together enhance proliferation of progenitor cells and suppress differentiation. (wikipedia.org)
Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. (nih.gov)
and positive regulation of cell differentiation. (mcw.edu)
A research team from the University Hospitals of Cleveland Medical Center in western Pennsylvania has discovered the existence of myeloid neoplasms (MNs) that can undergo plasmacytoid dendritic cell (PDC) differentiation. (news-medical.net)
Both categories of cells resulting from atypical PDC differentiation are thought to represent two ends of a spectrum of maturation. (news-medical.net)
Leukemic myoblast differentiation into PDCs occurs as a result of sharing a common hematopoietic precursor to dendritic cells, monocytes, and myeloid cells. (news-medical.net)
Beck's team further determined that the differentiation of PDC's may occur in the presence of monocytic or myeloid DC differentiation, or as a differentiation lineage possessing a predominant myoblast population. (news-medical.net)
Scientists at the University of Pennsylvania School of Veterinary Medicine have found a new wrinkle in the developmental biology dogma that cell differentiation occurs irreversibly as stem cells give rise to increasingly specialized types of offspring cells. (innovations-report.com)
Trajectory inference revealed that altered gene expression dynamics accompanied cell differentiation, which could explain age-associated changes in hematopoiesis. (cun.es)
Using previous findings in healthy cells as a reference, we analyzed scRNA-seq data obtained from patients with myelodysplastic syndrome (MDS) and detected specific alterations of the expression dynamics of genes involved in erythroid differentiation in all patients with MDS such as TRIB2. (cun.es)
In summary, we demonstrate that the combination of single cell technologies with computational analysis tools enable the study of a variety of cellular mechanisms involved in complex biological systems such as early hematopoiesis and can be used to dissect perturbed differentiation trajectories associated with perturbations such as aging and malignant transformation. (cun.es)
The ability to perform lineage tracing at the single-cell level is critical to reconstructing dynamic transitions during cell differentiation. (biorxiv.org)
Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. (duke.edu)
A variety of cytokines act synergistically with SCF to stimulate proliferation and differentiation of bone marrow progenitor cells. (southernbiotech.com)
For example, SCF plus IL-7 can stimulate the combined myeloid and B cell differentiation of uncommitted hematopoietic progenitor cells. (southernbiotech.com)
Hierarchical differentiation competence in response to retinoic acid ensures stem cell maintenance during mouse spermatogenesis. (southernbiotech.com)

CFU-S divides into two lineages: the lymphoid precursor (CFU-LSC) and the myeloid precursor (CFU-GEMM). (wikipedia.org)
In the adjacent image, CFU-GEMM is the scientific name for the "common myeloid progenitor" that is responsible for forming all the cells of the myeloid lineages. (wikipedia.org)
Pushing mature cells into other lineages may offer a new way to replace cells involved in blood diseases and neurodegenerative disorders such as Alzheimer s disease. (innovations-report.com)
In a murine competitive reconstitution model, Mybl2 knockdown by RNAi to 20-30% of normal levels in multipotent hematopoietic progenitors resulted in clonal dominance of these 'sub-haploinsufficient' cells, which was reflected in all blood cell lineages. (elifesciences.org)
Early hematopoiesis is a continuous process in which hematopoietic stem and progenitor cells (HSPCs) gradually differentiate toward specific lineages. (cun.es)
GATA-1 and PU.1 then initiate the presumed cascade of It governs the erythroid - myeloid lineages and demonstrates both transcriptional changes that culminate in the specification of the commitment as well as priming features [6,7]. (lu.se)

Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. (nih.gov)
Signaling through SCF/c-Kit has an important role in stimulating myeloid and erythroid production of primitive hematopoietic progenitor cells. (southernbiotech.com)

Single-cell analysis of mixed-lineage states leading to a binary cell fate choice. (uchicago.edu)

The researchers have shown that certain mouse cells retain an ability to oscillate between very distinct blood cell types - B-cells and macrophages - long after what has been commonly regarded as the point of no return. (innovations-report.com)
We suspect that this phenomenon is not limited to B-cells and macrophages in mice. (innovations-report.com)
It s possible that by targeting this gene with drugs, we may be able to convert malignant B-cell lymphomas into much less harmful histiocytomas, tumors composed of relatively inactive macrophages. (innovations-report.com)
Previous research has demonstrated that when B-cells are transplanted from an animal into a petri dish, the cells sometimes change into macrophages. (innovations-report.com)
Moreover, the cells could continue oscillating between B-cells and macrophages indefinitely, as many times as they were relocated. (innovations-report.com)
B-cells arise from a lymphoid progenitor, while macrophages come from a myeloid progenitor. (innovations-report.com)
To identify cell surface proteins on leukemia cells that inhibit Natural killer cells ( Aim 1a ) or macrophages ( Aim 1b ) using CRISPR screens. (lu.se)

Research in the Paralkar Lab spans the spectrum from human patient sample studies and mouse models to cutting-edge molecular biology tools, high-throughput sequencing approaches, and novel computational algorithms, all with the goal of gaining insight into how the transcription of coding genes and noncoding ribosomal DNA genes is regulated in hematopoietic stem cells, myeloid progenitors, and in leukemia. (upenn.edu)

We conclude that downregulation of MYBL2 activity below levels predicted by classical haploinsufficiency underlies the clonal expansion of hematopoietic progenitors in a large fraction of human myeloid malignancies. (elifesciences.org)
This deletion is only ever seen in one of their two copies of the chromosome-suggesting that at least some of these genes are essential for survival-but the identity of the gene(s) that are associated with the increased risk of myeloid malignancies is unknown. (elifesciences.org)
used gene silencing techniques to reduce the expression of MYBL2 in mice and showed that this induced symptoms of myeloid malignancies in the animals. (elifesciences.org)
In addition to revealing a new tumor suppressor gene and its contribution to myeloid malignancies, the study by Heinrichs et al. (elifesciences.org)
Imetelstat is a novel, first-in-class telomerase inhibitor exclusively owned by Geron and being developed in hematologic myeloid malignancies. (globenewswire.com)
Geron is a clinical stage biopharmaceutical company focused on the development and potential commercialization of a first-in-class telomerase inhibitor, imetelstat, in hematologic myeloid malignancies. (globenewswire.com)
Furthermore, the identification of abnormal regulatory mechanisms associated with myeloid malignancies could be exploited for personalized therapeutic approaches in individual patients. (cun.es)

This contrasts with the mouse, where polySia and NCAM expression are restricted to multipotent hematopoietic progenitors and cells developing along a myeloid lineage. (ca.gov)

ADAR1 silencing in breast cancer cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. (cancerindex.org)
While apoptosis and DNA damage response to replicative stress is similar between the control and SAA-iPSC-derived-hematopoietic progenitors, the latter show impaired proliferation which was not restored by eltrombopag, a drug which has been shown to restore hematopoiesis in SAA patients. (duke.edu)

Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
The endothelial cells that form the lining of these blood vessels are also a major part of the bone marrow niche. (medicalnewstoday.com)

RESULTS: Under our experimental conditions it was found that class III and class II CD34 epitopes (differentially resistant to enzymatic cleavage with neuraminidase, chymopapain and glycoprotease) are better preserved than class I epitope Eth sensitive to degradation Eth after cell exposure to cryoprotectant DMSO and the freezing- thawing procedures. (unimore.it)
A significant increase in CD34 antigen expression levels (i.e. antibody binding capacity, ABC) by cryopreserved cells stained with CD34 epitope class III, and class II reactive MoAbs was also documented, while no changes after cryopreservation were noted using class I-reactive MoAbs. (unimore.it)
These findings need to considered when selecting CD34 MoAbs for enumeration and positive selection of stem/progenitor cells for research and clinical purposes. (unimore.it)
The cells are characterized by expressing the cell surface markers CD33, CD34 and HLA-DR. These surface markers are proteins on the surface that are unique to specific cells and certain maturation periods, allowing researchers to differentiate between two different cells as well as what stage the cell is found in its developmental progression. (wikipedia.org)
In both analysis types, all PDCs showed partial to moderate expression of the cell marker CD34 indicating premature PDCs. (news-medical.net)
We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)

In this study, we used single cell RNA sequencing (scRNAseq) to characterize an enriched population of human hematopoietic stem and progenitor cells (HSPCs) obtained from young and elderly healthy individuals. (cun.es)

The group analyzed seven MN patients who presented with PDC proliferations comprising 5-26% of that blood cell or bone marrow. (news-medical.net)
Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
most of the neutrophils in the body are contained in the bone marrow, either as mitotically active (one third) or postmitotic mature cells (two thirds). (medscape.com)
Blood cells are produced within bone marrow by specialized stem cells and progenitor cells. (elifesciences.org)
When blood-forming hematopoietic stem cells that reside in the bone marrow age, the production of blood cells is altered, and the body's ability to regenerate lost cells is impaired. (medicalnewstoday.com)
A recent study in mice shows that aging is linked to the inflammation of cells in the bone marrow. (medicalnewstoday.com)
The bone marrow is the main site for hematopoiesis , the process of new blood cells forming via the hematopoietic stem cells (HSC) dividing and differentiating. (medicalnewstoday.com)
All these cells, along with blood vessels and the extracellular matrix, form the bone marrow niche . (medicalnewstoday.com)
The bone marrow niche includes cells belonging to the stroma, which includes mesenchymal stromal cells , adipocytes (fat cells), nerve cells, connective tissue cells, and cells involved in bone formation and remodeling. (medicalnewstoday.com)
Mesenchymal stromal cells can divide and differentiate into a number of cell types, including bone, cartilage, and adipocytes. (medicalnewstoday.com)
The study found that bone marrow niche cells that support the functioning of HSCs played a key role in blood aging. (medicalnewstoday.com)
In the present study, the researchers performed several analysis in the hindlimb, forelimb, and pelvic bones from young and old mice to examine the impact of aging on the interaction between the bone marrow niche and hematopoietic cells. (medicalnewstoday.com)
The present study found that aged mice showed a decrease in the population of mesenchymal stromal cells and osteoprogenitor cells that are involved in bone formation in the endosteum. (medicalnewstoday.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. (nih.gov)
Ma DD , 2007 , 'Expression of Neurofilament Proteins in Adult Bone Marrow Mesenchymal Stem Cells and Embryonic Stem Cells and Their Clinical Potential' , in Davenport LP (ed. (edu.au)
Peripheral blood contamination in human bone marrow aspirates was calculated from the ratios between the erythrocyte and nucleated cell counts in both the bone marrow aspirate and a simultaneously obtained peripheral blood sample. (karger.com)
C57BL/6 mouse bone marrow cells were stained with Rat Anti-Mouse CD117-BIOT (SB Cat. (southernbiotech.com)
4. Hodek M, Vávrová J, Šinkorová Z, Mokŕy J, Filip S. Hematopoietic recovery after transplantation CD117 + B220 - (LacZ + ) bone marrow cells in lethally irradiated mice. (southernbiotech.com)
Splenectomy influences homing of transplanted stem cells in bone marrow-ablated mice. (southernbiotech.com)
Normal hematopoiesis requires an intricate balance in the bone marrow between the ability of stem cells to maintain themselves for decades of life while producing billions of mature blood cells every day. (upenn.edu)

Minimal residual disease (MRD) refers to the small number of malignant cells that remain after therapy when the patient is in remission and shows no symptoms or overt signs of disease. (smw.ch)
The abstract reports results from early, nonclinical experiments on the potential effect of combining imetelstat and ruxolitinib on malignant myelofibrosis (MF) cells. (globenewswire.com)
As stated in the abstract, these findings suggest that an additive inhibitory activity against malignant myelofibrosis hematopoietic stem and progenitor cells can be achieved using a sequential treatment regimen of ruxolitinib followed by imetelstat. (globenewswire.com)
Early clinical data suggest imetelstat may have disease-modifying activity through the suppression of malignant progenitor cell clone proliferation, which allows potential recovery of normal hematopoiesis. (globenewswire.com)
Aging and myeloid malignant transformation are characterized by changes in the composition and regulation of HSPCs. (cun.es)
His research focus is on haematopoietic stem cell transplantation (HSCT) and translational research of normal and malignant stem cells. (edu.au)
The Paralkar Lab has developed customized genomes and computational pipelines to map datasets to rDNA, and we are interested in developing advanced tools to map and interpret the genetic and epigenetic profiles of rDNA in normal and malignant cells. (upenn.edu)

It matures into the megakaryocyte, erythrocyte, mast cell or myeloblast based on the presence of specific factors that encourage the cell to choose a lineage to follow. (wikipedia.org)
The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. (nih.gov)

For both of these groups, DNA was extracted for analysis from serum specimens and peripheral blood mononuclear cells (PBMCs). (cdc.gov)
Groups 3 and 4 comprised recipients of kidney and allogeneic BM/peripheral blood stem cell (PBSC) transplants, for which DNA was extracted from serum specimens collected at 6 and 12 months, respectively, after transplantation. (cdc.gov)

Mice with Socs3 deleted specifically in hematopoietic cells succumb to severe neutrophil and macrophage-driven inflammation by 1 year of age, and responses to G-CSF are grossly exacerbated. (nih.gov)
By 6 months post-transplantation, the reconstituted mice had developed a clonal myeloproliferative/myelodysplastic disorder originating from the cells with aberrantly reduced Mybl2 expression. (elifesciences.org)
Moreover, injection of modified cells from these animals into healthy mice also induced symptoms in the recipients. (elifesciences.org)
A recent study conducted in mice shows that blocking the receptor for the proinflammatory protein IL-1 using the FDA-approved rheumatoid arthritis drug Anakinra may attenuate the deficits in the functioning of hematopoietic stem cells due to aging. (medicalnewstoday.com)
In contrast, there was an increase in the number of mesenchymal stromal cells in the central marrow of aged mice. (medicalnewstoday.com)
T cells in cryptopatch aggregates share TCR γ variable region junctional sequences with γδ T cells in the small intestinal epithelium of mice. (southernbiotech.com)

PDCs are a class of specialized dendritic cells that play a role in immunomodulatory functions and antigen presentation. (news-medical.net)
Dendritic cells (DCs) are specialized antigen-presenting cells that have a notable role in the initiation and regulation of innate and adaptive immune responses. (mdpi.com)

However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)

It unlocks novel dimensions by uncovering elusive cell populations and tumor heterogeneities for early detection, unraveling mechanisms of treatment resistance, tailoring therapies to individuals, and illuminating metastasis and cachexia pathways, thereby opening fresh avenues for preventive strategies. (frontiersin.org)
Please refer to the Separation Flow Chart to identify the separated cell populations represented in this figure. (bdbiosciences.com)
Besides housing hematopoietic stem cells and their progeny, the endosteum and the central marrow also contain distinct cell populations that express molecules or secrete proteins, such as cytokines that regulate the formation, development, and function of HSCs and progenitor cells. (medicalnewstoday.com)

This study revealed that IL-1 plays an important role in the regulation of the production of stimulatory factors that influence the progenitor cells of hematopoiesis. (wikipedia.org)
The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
The Paralkar Lab has identified that key hematopoietic and leukemic transcription factors bind to rDNA and regulate rRNA transcription, and we are interested in understanding how the binding of cell-type-specific transcription factors regulates the activity of Polymerase I and the transcription of rRNA in normal hematopoiesis, and how this regulation is co-opted in leukemia to drive abundant ribosome biogenesis. (upenn.edu)

Preliminary studies show that a vaccine made with leukemia cells may be able to reduce or eliminate the last remaining cancer cells in some chronic myeloid leukemia patients taking the drug Imatinib mesylate (Gleevec). (sciencedaily.com)

Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of quiescent cells which are to some extent resistant to tyrosine kinase inhibitors (TKIs). (oncotarget.com)
For this study, researchers analyzed leukemia cells from 111 St. Jude AML patients representing the seven most common subtypes of the disease. (scienceblog.com)
I will accomplish this by identifying and mechanistically characterize cell surface proteins that protect leukemia cells against the innate immune system. (lu.se)
I will also provide in vivo proof of concept for a therapeutic effect by inhibiting the identified cell surface proteins in mouse models of leukemia. (lu.se)
To demonstrate proof of concept for a therapeutic effect by inhibiting the identified cell surface proteins in patient-derived xenograft models of leukemia. (lu.se)

Single-cell sequencing is a high-throughput technique that enables detection of genomic, transcriptomic, and epigenomic information at the individual cell level, offering significant advantages in detecting cellular heterogeneity, precise cell classification, and identifying rare subpopulations. (frontiersin.org)

Cytokine-induced expression of transcriptional regulators including cebpb, Ets2, Bcl3, c-Myc, Jun, and Fosl2 are differentially regulated in Socs3-deficient cells. (nih.gov)
Based on their transcriptional profile, we identified changes in the proportions of progenitor compartments during aging, and differences in their functionality, as evidenced by gene set enrichment analysis. (cun.es)
Given that rRNA accounts for the bulk of the transcriptional output of the cell, the inability to map datasets to rDNA has historically been a major limitation, and has created a significant knowledge gap in our understanding of the most abundant RNA in the cell. (upenn.edu)

Strikingly, synergistic responses to costimulation with stem cell factor and IL-6 (but not G-CSF) are lost at higher concentrations in Socs3-deficient progenitor cells. (nih.gov)
The growth of CFU-GEMM is stimulated by the stem cell factor, or SCF. (wikipedia.org)
The stem cell will follow a specific lineage depending on the presence of certain growth factors and cytokines. (wikipedia.org)
The results of studies conducted by Carow, Hangoc, and Broxmeyer in 1993 reveal that the CFU-GEMM can be classified as a stem cell due to its high replating efficiency in the presence of certain growth factors and cytokines. (wikipedia.org)
02:32 what makes us Ella stem cell? (yale.edu)
Pre-emptive treatment with rituximab of molecular relapse after autologous stem cell transplantation in mantle cell lymphoma. (smw.ch)
Hematopoietic stem cell lineage choices are decided by genetic networks that are turned ON/OFF in a switch-like manner. (lu.se)
It levels for the hematopoietic stem cell system [1,2]. (lu.se)
Professor Ma heads the Blood, Stem Cell and Cancer Research Programme at St Vincent's Centre for Applied Medical Research, Sydney. (edu.au)
Stem Cell Research Advances , edn. (edu.au)
The CD117 (c-Kit) antigen is the cell-surface receptor for stem cell factor (SCF). (southernbiotech.com)

These findings support senescent cells as a promising druggable target to counteract the SASP and skeletal muscle wasting in the context of chemotherapy. (jci.org)

BCR-ABL oncogene activates multiple cross-talking signal transduction pathways (STP), such as RAS/MEK/ERK, PI3K/Akt, Wnt and STAT5, contributing to abnormal proliferation of clonal cells. (oncotarget.com)
We further applied mitochondrial variant lineage tracing to spatial transcriptomics and identified the clonal development of both erythro-myeloid progenitors and their niche cells. (biorxiv.org)
Our study highlights the power of mitochondrial variants as an endogenous marker for clonal tracking in modeling human development from stem cells. (biorxiv.org)

In current terminology, CFU-S refers to the pluripotent stem cells that can differentiate into all types of blood cells. (wikipedia.org)

In order to determine which elements of cellular responses to cytokines require Socs3, we have examined the differentiative and proliferative capacity of hematopoietic progenitor cells stimulated by G-CSF and IL-6. (nih.gov)
Cells were cultured for up to 5 days in IMDM medium supplemented with 10% FCS/2% PSG/β-me and IL-7 and SCF cytokines on S17 feeder cells in the presence of 1 ug/mL doxycycline, or alternatively, in alpha-MEM medium in the presence of cytokines and on Tst-4 stromal cells. (nih.gov)

02:47 and red blood cells in vitro. (yale.edu)
Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
Importantly, lactate incubation in vitro on Type II cells did not significantly increase the inflammatory byproduct IL-1β. (jci.org)
Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)

MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Stromal cell feeder layers (e.g. (stemcell.com)

Clusters of tumor cells separated by a hyaline and mucin-rich stroma (hematoxylin and eosin [H&E] stain, medium power). (medscape.com)
High-power magnification with CD-34 antigen immunohistochemical stain showing membrane positivity of the tumor cells. (medscape.com)
Regardless of the therapeutic approach, the heterogeneity of pancreatic cancer cells and the complexity of its tumor microenvironment contribute to the emergence of drug resistance. (frontiersin.org)
The modified cells are able to expand more robustly than normal cells, and this dominance induced by downregulation of the tumor suppressor increases the risk of malignancy. (elifesciences.org)
In the context of cancer, appropriately activated DCs can induce anti-tumor immunity by activating innate immune cells and tumor-specific lymphocytes that target cancer cells. (mdpi.com)
CD117 and Stro-1 identify osteosarcoma tumor-initiating cells associated with metastasis and drug resistance. (southernbiotech.com)

Emerging single-cell sequencing technologies will undoubtedly enhance our understanding of the complex biology of pancreatic cancer and pave the way for new directions in its clinical diagnosis and treatment. (frontiersin.org)
In a pilot study published in Clinical Cancer Research , the Johns Hopkins investigators used a vaccine made from CML cells irradiated to halt their cancerous potential and genetically altered to produce an immune system stimulator called GM-CSF. (sciencedaily.com)

Our results showed that CP-CML CD34+ progenitors were characterized by significant lower phosphorylation of proteins involved in the regulation of growth and cell survival, such as tyrosine kinases of the Src family and members of STAT family, and by a significant higher phosphorylation of p53 (Ser15), compared to normal CD34+ cells from healthy donors. (oncotarget.com)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
In another study in 2014, researchers were in search of molecules to stimulate the proliferation of long-term hematopoietic stem cells (LT-HSC). (wikipedia.org)
The treated cells also carry molecules, called antigens, specific to CML cells, which prime the immune system to recognize and kill circulating CML cells. (sciencedaily.com)

Krutzik, P.O. & Nolan, G.P. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. (nature.com)

CFU-GEMM is a colony forming unit that generates myeloid cells. (wikipedia.org)

A similar compound, sunitinib (Sutent), has been shown to be effective in patients with mutant GIST cells that are resistant to imatinib mesylate. (medscape.com)

We explored the use of naturally occurring somatic mutations in mitochondrial transcripts detected in single-cell RNA-seq as genetic lineage barcodes in HPSC. (biorxiv.org)
Current bioinformatic pipelines for high-throughput studies like whole genome sequencing, RNA-seq, ChIP-seq, and single cell RNA-seq are limited in their ability to map repetitive elements of the genome like ribosomal DNA. (upenn.edu)

Senescent cells exhibit chronic activation of the transcription factor nuclear factor (NF)-κB, a known mediator of the pro-inflammatory senescence-associated secretory phenotype (SASP) and skeletal muscle atrophy. (jci.org)
Although the immunological nature of SAA pathogenesis is widely accepted, there is an increasing recognition of the role of dysfunctional hematopoietic stem cells in the disease phenotype. (duke.edu)

Cutting Edge: Lymphomyeloid-Primed Progenitor Cell Fates Are Controlled by the Transcription Factor Tal1. (uchicago.edu)
However, prior to commitment, it has been cells, it is evident that GATA-1 and PU.1 are able to specify observed that many genes are expressed at intermediate or basal erythroid and myeloid cell fates (see [16] and references therein). (lu.se)

Analysis of expression profile on proteins involved in the apoptotic machinery revealed that, in addition, CD34+ cells from CP-CML were characterized by a significant lower expression of catalase and higher expression of HSP27 and FADD. (oncotarget.com)

The tight regulation by Socs3 of signal transducer and activator of transcription 3 phosphorylation and gene transcription after cytokine receptor ligation significantly influences the fate of myeloid progenitor cells. (nih.gov)
The gene, which is called MYBL2 , encodes a transcription factor that helps to control the cell division cycle. (elifesciences.org)
Myeloid malignancy patients lacking one copy of this gene showed levels of MYBL2 expression that were less than 50% of those in healthy individuals. (elifesciences.org)

This is the first demonstration of polySia expression and regulation on myeloid cells, and the results in animal models suggest a role for polySia in immune regulation. (ca.gov)

Gleevec, one of the first targeted cancer therapies with wide success in CML patients, destroys most leukemic cells in the body, but in most patients, some cancerous cells remain and are measurable with sensitive molecular tests. (sciencedaily.com)
The study vaccine was given to 19 CML patients with measurable cancer cells, despite taking Gleevec for at least one year. (sciencedaily.com)
After a median of 72 months of follow-up, the number of remaining cancer cells declined in 13 patients, 12 of whom reached their lowest levels of residual cancer cells. (sciencedaily.com)
Often patients have low blood cell counts, fluid retention, significant nausea and other gastrointestinal problems," says B. Douglas Smith, M.D., associate professor of oncology at the Johns Hopkins Kimmel Cancer Center. (sciencedaily.com)
The number and function of proangiogenic cells are impaired in patients with diabetes or cardiovascular diseases. (hindawi.com)
B19 is the primary etiologic agent causing TAC in patients with chronic hemolytic anemias (e.g., sickle cell disease, hemoglobin SC disease, hereditary spherocytosis, alpha-thalassemia, and autoimmune hemolytic anemia) (22,23). (cdc.gov)

It has been suggested that eosinophils do not derive from the common myeloid progenitor in humans. (wikipedia.org)

Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). (bdbiosciences.com)

rRNA transcription rates vary dramatically between different normal cell types in the hematopoietic tree, and leukemic cells have characteristic prominent nucleoli, indicating robust ribosome synthesis. (upenn.edu)

Anatomy68

Organisms14

Diseases11

Chemicals and Drugs86

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes44

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science2

Acute19

Differentiation19

Lineages6

Primitive hematopoietic progenitor cells2

Mixed-lineage1

Macrophages7

Regulated in hematopoietic stem1

Malignancies7

Multipotent hematopoietic progenitors1

Apoptosis2

Endothelial cells4

CD3411

Human Hematopoietic Stem1

Bone22

Malignant7

Megakaryocyte2

Peripheral2

Mice6

DENDRITIC CELLS2

Monocytes1

Populations3

Hematopoiesis3

Chronic myeloid leukemia1

Leukemia5

Subpopulations1

Transcriptional3

Stem cell11

Chemotherapy1

Clonal3

Differentiate1

Cytokines2

Vitro6

Stromal3

Tumor6

Clinical2

Tyrosine1

Molecules3

Flow cytometry1

Colony form1

Imatinib1

Single-cell RNA-seq2

Phenotype2

Fates2

Proteins1

Gene3

Regulation1

Patients6

Derive1

Fraction1

Leukemic cells1