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  • HSCs
  • Hematopoietic stem cells (HSCs) reside in specialized bone marrow (BM) niches regulated by the sympathetic nervous system (SNS). (nih.gov)
  • Using MO and MΦ conditional depletion models, we found that reductions in BM mononuclear phagocytes led to reduced BM CXCL12 levels, the selective down-regulation of HSC retention genes in Nestin(+) niche cells , and egress of HSCs/progenitors to the bloodstream. (nih.gov)
  • These results highlight two antagonistic, tightly balanced pathways that regulate maintenance of HSCs/progenitors in the niche during homeostasis, in which MΦ cross talk with the Nestin(+) niche cell promotes retention , and in contrast, SNS signals enhance egress. (nih.gov)
  • Pluripotent, Lin - /CD45 - /Sca-1 + very small embryonic-like stem cells (VSELs) in mouse bone marrow (BM) are resistant to total body radiation because of their quiescent nature, whereas Lin - /CD45 + /Sca-1 + hematopoietic stem cells (HSCs) get eliminated. (biomedcentral.com)
  • Nuclear OCT-4, SCA-1, and SSEA-1 coexpressing LIN - /CD45 - VSELs and slightly larger LIN - /CD45 + HSCs expressing cytoplasmic OCT-4 were identified and comprised 0.022 ± 0.002 % and 0.081 ± 0.004 % respectively of the total cells in BM. (biomedcentral.com)
  • VSELs are the most primitive pluripotent stem cells in BM that self-renew and give rise to HSCs under stress, and HSCs further divide rapidly and differentiate to maintain homeostasis. (biomedcentral.com)
  • The bone marrow, a highly vascularized fibrous tissue, is the physiological niche of hematopoietic stem cells (HSCs) in the adult. (hubrecht.eu)
  • There, HSCs can self-renew or differentiate into hematopoietic progenitor cells (HPCs) to produce daily all mature blood cells needed throughout life. (hubrecht.eu)
  • transplantation
  • For many decades, bone marrow (BM) transplantation was the only viable method for transplanting hematopoietic stem cells, although their presence had been demonstrated in peripheral blood. (hindawi.com)
  • and the number of contaminated T cells was considered too high for safe allogeneic transplantation [ 1 ]. (hindawi.com)
  • The successful transplantation of hematopoietic stem/progenitor cells (HSPCs) is based on their ability to home to the BM niche and on their engraftment capacity. (hindawi.com)
  • The use of mobilized peripheral blood is now the method of choice in autologous transplantation for various reasons, including an elevated production of immature cells, and, in comparison to the utilization of BM, the shorter time period required for a satisfactory repopulation, the more rapid engraftment, fewer technical difficulties, lower risk, and considerably less pain [ 6 ]. (hindawi.com)
  • Following lethal dose-irradiation and subsequent green fluorescent protein-transgenic bone marrow cell-transplantation (GFP-BMT) in mice, a BMP-2-containing collagen pellet was implanted into muscle. (osti.gov)
  • EPC transplantation significantly reduced brain edema and the number of dead cells in the hematoma boundary areas. (frontiersin.org)
  • mice
  • In the OIR retinas of BM-transplanted mice with BM-LS cells of EGFP transgenic mice, there was less incorporation of GFP + cells into vascular structures at P12, whereas there was a drastic recruitment into the "tufts " and for the intact vasculature at P17. (arvojournals.org)
  • C ) Role of selectins in FDCP-mix cell rolling in wild-type mice. (rupress.org)
  • Groups were compared with control using the Kruskal-Wallis test followed by Bonferoni correction of P . ( D ) Comparison of FDCP-mix cell rolling in wild-type and P/E-selectin double knockout mice. (rupress.org)
  • The mobilization of vasculogenic progenitors required the presence of functionally active osteoclasts, as demonstrated in PTPε-deficient mice with defective osteoclast function. (ahajournals.org)
  • autologous
  • D. Enea, S. Cecconi, S. Calcagno, A. Busilacchi, S. Manzotti, and A. Gigante, "One-step cartilage repair in the knee: collagen-covered microfracture and autologous bone marrow concentrate. (hindawi.com)
  • The safety of the administration of autologous bone marrow-derived EPC in patients with advanced cirrhosis will be evaluated by anamnesis, physical examination, hematological and biochemical variables and imaging examination. (clinicaltrials.gov)
  • lymphoid
  • This combination of constitutively expressed adhesion molecules may thus constitute a BM-specific recruitment pathway for progenitor cells analogous to the vascular addressins that direct selective lymphocyte homing to lymphoid organs. (rupress.org)
  • Niches
  • F. M. Watt and B. L. M. Hogan, "Out of Eden: Stem Cells and Their Niches," Science, 284, 1427-1430 (2000). (google.com)
  • Under steady-state conditions, a small amount of hematopoietic stem cells constantly leave the BM and penetrate tissues, returning to the BM or peripheral niches via the blood or lymphatic system [ 2 ]. (hindawi.com)
  • Our engineered primary 3D bone marrow model provides an easy and reliable model to further investigate the interactions between HSPCs and their surrounding niches (i.e. endosteal and perivascular niches). (hubrecht.eu)
  • Overall, the 3D model will be useful to study the role of bone marrow niches in normal hematopoiesis as well as in the case of bone marrow related diseases. (hubrecht.eu)
  • whereas
  • DM increased PGC-1α mRNA in WT BM-KSL cells whereas this induction was blocked in KO cells. (ahajournals.org)
  • Eight of 20 group A patients (40%) achieved the primary endpoint of mature bone regeneration, whereas all 20 group B patients (100%) achieved the primary endpoint. (quintpub.com)
  • Whereas osteoblasts regulate hematopoiesis, 14,15 osteoclasts, as well as the release of the cytokine RANKL, which increases osteoclastogenesis, were associated with mobilization of hematopoietic progenitor cells. (ahajournals.org)
  • Whereas KL and FL in combination stimulated the clonal growth of only 3% of CD34+ CD38- cells, 40% of CD34+ CD38- cells were recruited by KL+FL+Tpo, demonstrating that Tpo promotes the growth of a high fraction of CD34+ CD38- progenitor cells. (ox.ac.uk)
  • CD38
  • Finally, we identified inhibitors of Tpo-induced progenitor cell growth, in that TGF-beta as well as TNF-alpha almost completely abrogated the growth of CD34+ CD38- progenitor cells in response to Tpo alone as well as KL+FL+Tpo. (ox.ac.uk)
  • hematopoiesis
  • No. 5,811,301, for example, the disclosure of which is hereby incorporated by reference, describes the process of hematopoiesis, the development of the various cells that comprise blood. (google.com)
  • 5-FU treatment resulted in depletion of cells with a 7-fold reduction by D4 and normal hematopoiesis was re-established by D10. (biomedcentral.com)
  • Till date, however, the role of VSELs in hematopoiesis in vivo either in steady-state conditions or in response to bone marrow injury has not been studied extensively. (biomedcentral.com)
  • vascular
  • Moreover, the definitive EPC colony cells intravitreally injected into OIR significantly abrogated pathologic versus primitive vascular growth. (arvojournals.org)
  • Selectin-independent rolling was mediated by α4 integrins, which interacted with endothelial vascular cell adhesion molecule (VCAM)-1. (rupress.org)
  • 2-4 The vasculogenic capacity of bone marrow-derived cells acting as vascular modulators may in part rely on the secretion of angiogenic growth factors without being necessarily integrated into vessels. (ahajournals.org)
  • modulates
  • As a secondary aim, diabetic patients will be divided in those with and without diabetic autonomic neuropathy (DAN) to understand if DAN modulates bone marrow responsiveness to G-CSF. (clinicaltrials.gov)
  • tissues
  • The prior art describes the development, from stem cell to differentiated cells, of various tissues throughout the body. (google.com)
  • Parallel contribution of the endothelial selectins and VCAM-1 is not known to direct blood cell trafficking to other noninflamed tissues. (rupress.org)
  • Patients
  • In the present study, we aim at investigating bone marrow responsiveness to pharmacological mobilization of CPC in diabetic patients as compared to non-diabetic subjects. (clinicaltrials.gov)
  • Patients with active infectious or neoplastic disease, a history of cytotoxic or radiation therapy, primary or secondary metabolic bone disease, or bone marrow dysfunction were excluded. (hindawi.com)
  • and (d) the effects of this mobilization on BM-derived stem/progenitor cells in clinical trials of patients with different diseases. (hindawi.com)
  • These findings, for the first time, document activation of the stem cell niche within the bone marrow following AMI, which may have important implications for the optimal timing of cell therapy using BMC in patients with AMI. (ahajournals.org)
  • Using a double-blind, placebo controlled multicenter trial design, we randomized 204 patients with successfully reperfused acute myocardial infarction to receive intracoronary infusion of bone marrow - derived progenitor cells (BMC, n = 101) or placebo medium (n= 103) into the reperfused infarct artery. (ahajournals.org)
  • Intracoronary infusion of bone marrow-derived progenitor cells is associated with enhanced efficacy in patients with the most severe depression of left ventricular function as well as when performed at ≥ 5 days after AMI reperfusion therapy. (ahajournals.org)
  • In contrast to previous studies, we demonstrate herein that systemic SDF-1 levels do not seem to be linked with increased mobilization of stem cells in patients with pancreatic cancer. (edu.pl)
  • Cells of the monocyte-macrophage lineage are a major target of human immunodeficiency virus type 1 (HIV-1), and play a crucial role in the dissemination of the virus and the development of disease symptomatology, AIDS, HIV-1-associated dementia (HAD), and perhaps other HIV-1-associated CNS disorders observed in patients treated with highly active antiretroviral therapy (HAART) for prolonged periods of time [1, (medcraveonline.com)
  • clinical
  • Cell-free collagen type I matrix for repair of cartilage defects-clinical and magnetic resonance imaging results," Knee Surgery, Sports Traumatology, Arthroscopy , vol. 20, no. 10, pp. 1915-1922, 2012. (hindawi.com)
  • This method enables us to obtain large numbers of BMPCs for both research and clinical use from a relatively small sample of bone marrow aspirate. (hindawi.com)
  • So far, there are no clinical data demonstrating alterations in the bone marrow (BM) niche after AMI, which may have a functional impact on BM-resident progenitor cells. (ahajournals.org)
  • Thus, these data show that Dkk-1 is a mobilizer of vasculogenic progenitors but not of inflammatory cells, which could be of great clinical importance to enhance regenerative cell therapy. (ahajournals.org)
  • All reagents and devices used to collect and separate the cells are certified clinical grade. (stemexpress.com)
  • Circulation
  • Recent studies have suggested the existence of osteoblastic cells in the circulation, but the origin and role of these cells in vivo are not clear. (osti.gov)
  • tissue
  • Further methods of the present invention are directed to utilizing the neural progenitor cells cultured in this fashion in the treatment of various neuropathological conditions, and in targeting delivery of cells transfected with a particular gene to diseased or damaged tissue. (google.com)
  • It is the progenitor cells that proliferate into the various lymphocytes, neutrophils, macrophages, and other cells that comprise blood tissue of the body. (google.com)
  • A niche is a subgroup of tissue cells and extracellular substrates that can indefinitely harbor one or more stem cells and control their self-renewal and progeny in vivo [ 3 ]. (hindawi.com)
  • Group A received an in situ tissue-engineered graft containing 54 38 CD34+ cells/mL along with 54 38 CD44+, CD90+, and CD105+ cells/mL together with rhBMP-2 in an absorbable collagen sponge (1 mg/cm of defect) and crushed cancellous allogeneic bone. (quintpub.com)
  • infusion
  • The REPAIR-AMI trial has demonstrated that intracoronary infusion of bone marrow-derived progenitor cells (BMC) is associated with a larger recovery of left ventricular contractile function after acute myocardial infarction (AMI) compared to placebo. (ahajournals.org)
  • collagen
  • A collagen-hydroxyapatite scaffold allows for binding and co-delivery of recombinant bone morphogenetic proteins and bisphosphonates," Acta Biomaterialia , vol. 10, no. 5, pp. 2250-2258, 2014. (hindawi.com)
  • systemic
  • It is therefore important to note that a bone fracture, like any other musculoskeletal injury, should be considered a systemic event, resulting in a systemic response [ 25 ]. (hindawi.com)