• Herein, we demonstrate that GC conditioning during the differentiation of myeloid progenitors into macrophages (Mφs) results in their enhanced LPS responsiveness, demonstrated by an overexpression of the inflammatory cytokines TNF-α, IL-6, and IL-12. (aai.org)
  • Cord blood (CB) mononuclear cells (MNC) are being tested in clinical trials to treat hypoxic-ischemic (HI) brain injuries. (biorxiv.org)
  • Separation of chemotherapy plus G-CSF-mobilized peripheral blood mononuclear cells by counterflow centrifugal elutriation: in vitro characterization of two different CD34+ cell populations. (ntu.ac.uk)
  • To investigate the transcriptional diversity of BM-MSCs, we applied single-cell RNA sequencing (scRNA-seq) on freshly isolated CD271 + BM-derived mononuclear cells (BM-MNCs) from two human subjects. (ijbs.com)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • Enriched populations of CD34(+) cells were isolated by means of positive immunomagnetic selection with MACS beads. (dadamo.com)
  • Similarly, ATRA (10(-6) mol/L) inhibited eosinophil-basophil differentiation of cord blood CD34(+) cells in liquid culture, whereas neutrophil differentiation proceeded without impediment. (dadamo.com)
  • Most importantly, these effects of ATRA (10(-8) to 10(-6) mol/L) on CD34(+) cells were associated with a dose-dependent inhibition of IL-5Ralpha but no change in GM-CSF receptor expression, as detected with flow cytometry. (dadamo.com)
  • Retinoic acid modulates IL-5 receptor expression and selectively inhibits eosinophil-basophil differentiation of hemopoietic progenitor cells. (dadamo.com)
  • Currently, little is known concerning regulation of IL-5Ralpha expression in the context of commitment of hemopoietic progenitor cells to the eosinophil and basophil lineages. (dadamo.com)
  • Our results suggest that minor fluctuations in physiological levels of endogenous GCs can program endotoxin-responsive hemopoietic cells during their differentiation by regulating their sensitivity to stimulation. (aai.org)
  • rhG-CSF in healthy donors: mobilization of peripheral hemopoietic progenitors and effect on peripheral blood leukocytes. (ntu.ac.uk)
  • We screened a collection of 312 annotated antibodies and uncovered integrin-associated protein (IAP, Compact disc47) being a cell surface area marker ideal for immunomagnetic isolation of FOXA2+ hPSC-derived mesDA progenitor cells with flooring plate identity. (epf2013.org)
  • Materials and Methods Isolation tradition and illness of primary human being CD34+ cells and of U937 cells The human being hematopoietic cell collection U937 was cultured in RPMI-1640 (Existence Systems Carlsbad CA USA) comprising 10% fetal bovine serum (FBS) (Existence Technologies) inside a humidified incubator at 37°C and 5% CO2. (acancerjourney.info)
  • Morphological-based isolation separates CTCs according to size discrepancies, using isolation by size of epithelial tumor cells (ISET) or according to density, using density-gradient separation ( 4 ). (iiarjournals.org)
  • Immunological techniques, which are the most widely used methods, employ immunomagnetic isolation ( 4 ). (iiarjournals.org)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Furthermore, we demonstrated that these automatically isolated cells bear proliferation and differentiation capacities comparable to manually isolated cells in vitro. (biomedcentral.com)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
  • B) hESCs (H9) and hiPSCs (hFF-iPS) had been differentiated toward mesDA progenitor cells and screened for marker appearance on d11 and d16 of differentiation. (epf2013.org)
  • Human myelopoiesis is an exciting biological model for cellular differentiation since it represents a plastic process where multipotent stem cells gradually limit their differentiation potential, generating different precursor cells which finally evolve into distinct terminally differentiated cells. (biomedcentral.com)
  • In summary a number of reports have shown that manifestation of causes improved proliferation and delayed differentiation of murine hematopoietic stem and progenitor cells. (acancerjourney.info)
  • We consequently set out to study the manifestation and function of in main human being cluster of differentiation 34 positive (CD34+) hematopoietic progenitor cells. (acancerjourney.info)
  • and 0.5 ng/ml tumor necrosis factor α(TNFα) (all cytokines from Peprotech) for dendritic (Langerhans cell LC) differentiation. (acancerjourney.info)
  • For megakaryocyte differentiation cells had been extended for 6 times in StemPro-34 mass media (Life Technology) filled with 1 ng/ml IL-3 1 ng/ml SCF and 50 ng/ml TPO (all from Miltenyi Biotec Bergisch Gladbach Germany) and differentiated in mass media supplemented GF 109203X with 50 ng/ml TPO. (acancerjourney.info)
  • Differentiation was permitted to move forward for 7-13 times based on lineage and was supervised by FACS for lineage-specific cell surface area markers (Compact disc15 for granulocytes Compact disc14 for monocytes. (acancerjourney.info)
  • Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
  • We successfully identified LEPR hi CD45 low BM-MSCs within the CD271 + BM-MNC population, and further codified the BM-MSCs into distinct subpopulations corresponding to the osteogenic, chondrogenic, and adipogenic differentiation trajectories, as well as terminal-stage quiescent cells. (ijbs.com)
  • METHODS: Progenitor cells were obtained from bone marrow aspirates and cord blood samples. (dadamo.com)
  • Without limitation, some embodiments comprise methods, systems, and/or compositions relating to microRNAs and/or cell based therapies and the use of the same in the research, diagnosis, or treatment of injury or disease. (justia.com)
  • In this study, we addressed feeder cell-free expansion methods using common γ-chain cytokines, especially IL-15 and IL-21. (frontiersin.org)
  • An emerging method to detect metastasis is the analysis of circulating tumor cells (CTCs), which has shown promise in filling the gaps left by other diagnostic methods. (iiarjournals.org)
  • Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
  • The CliniMACS Plus Instrument (Miltenyi Biotec) utilizes immunomagnetic techniques to isolate hematopoietic progenitor. (atlantis-press.com)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • In addition, the regenerative potential of purified stem cells was assessed 3 weeks after transplantation in immunodeficient mice which had been subjected to experimental myocardial infarction. (biomedcentral.com)
  • Nevertheless, in these scholarly studies, cells had been either cultivated for a protracted time taken between sorting (time 12) Iopanoic acid and transplantation (time 28/42) (Doi et?al. (epf2013.org)
  • Endogenous cytokine levels after immunoselected CD34+ peripheral blood progenitor cell transplantation. (ntu.ac.uk)
  • Besides surgery, radiotherapy and chemotherapy, immune activation by direct application of cytokines, antibodies or adoptive cell therapy are promising approaches. (frontiersin.org)
  • In vivo development and survival of NK cells require cytokines ( 6 - 8 ). (frontiersin.org)
  • In this context, cytokines have been shown to activate NK cells potently during ex vivo expansion ( 9 - 12 ). (frontiersin.org)
  • Partial interspecies cross-reactivity between murine cytokines/chemokines or MHC and co-stimulatory molecules with their human cognate receptors, might reduce the survival capacity of engrafted human cells, possibly affecting the decay of the latent reservoir. (biomedcentral.com)
  • To explore these mechanisms further, we exposed mouse brain organotypic slice cultures to oxygen and glucose deprivation (OGD) and then treated the brain slices with cells from CB or adult peripheral blood (PB). (biorxiv.org)
  • RhG-CSF-mobilized peripheral blood haemopoietic progenitors reside in G0/G1 phase of cell cycle independently of the expression of myeloid antigens. (ntu.ac.uk)
  • Cell markers are very valuable tools for examining the function of cells in normal conditions as well as during disease. (wikipedia.org)
  • Although immunohistochemistry is the staple methodology for identifying neuronal cell types, since it is relatively low in cost and a wide range of immunohistochemical markers are available to help distinguish the phenotype of cells in the brain, sometimes it is time-consuming to produce a good antibody. (wikipedia.org)
  • Depending on the goal of a particular study, one can use neural stem cells markers, neural progenitor cell markers, neuron markers or PNS neuronal markers. (wikipedia.org)
  • Immunomagnetically sorted IAP+ mesDA progenitors demonstrated increased appearance of ventral midbrain flooring dish markers, lacked appearance of pluripotency markers, and differentiated into older dopaminergic (DA) neurons (Lindvall and Kokaia, 2009). (epf2013.org)
  • 2007) as mesDA progenitor-associated cell surface area markers. (epf2013.org)
  • Surface area markers portrayed on 90% from the FOXA2+ mesDA progenitor cells are depicted within the Edwards-Venn diagram (Bardou et?al. (epf2013.org)
  • Twelve surface area markers had been concomitantly portrayed on d11 and d16 both in hESC and hiPSC-derived FOXA2+ cells. (epf2013.org)
  • As proven for IAP, surface area markers portrayed by mesDA progenitors had been identified in line with the co-staining with FOXA2. (epf2013.org)
  • Background: Mature circulating endothelial cells (CEC) are surrogate markers of endothelial damage. (researchgate.net)
  • Mature circulating endothelial cells (CEC) are surrogate markers of endothelial damage. (researchgate.net)
  • This method uses either epithelial cell-specific markers which are generally expressed in all tumor cells, or tumor markers expressed by specific types of cancer ( 13 ). (iiarjournals.org)
  • In particular, we quantified the HIV DNA in hu-mice treated for different length with cART and explored whether it correlated with the viral load before cART, the number of target cells and immune activation markers. (biomedcentral.com)
  • Bone marrow-derived mesenchymal stem cells (BM-MSCs) are multipotent stromal cells that have a critical role in the maintenance of skeletal tissues such as bone, cartilage, and the fat in bone marrow. (ijbs.com)
  • Our results showed that CP-CML CD34+ progenitors were characterized by significant lower phosphorylation of proteins involved in the regulation of growth and cell survival, such as tyrosine kinases of the Src family and members of STAT family, and by a significant higher phosphorylation of p53 (Ser15), compared to normal CD34+ cells from healthy donors. (oncotarget.com)
  • CD34+ cells were isolated from umbilical wire blood (CB) from consenting donors using immunomagnetic beads (StemCell Systems Grenoble France). (acancerjourney.info)
  • 2007), steady engraftment, and success of transplanted embryonic stem cell (ESC)-produced neural cells (Barral et?al. (epf2013.org)
  • Chronic Myeloid Leukemia (CML) is a stem cell disease sustained by a rare population of quiescent cells which are to some extent resistant to tyrosine kinase inhibitors (TKIs). (oncotarget.com)
  • For instance, the existence of tissue-specific gene clusters may be related to the efficient activation of gene expression in a particular cell lineage, by genetic and epigenetic mechanisms, or related to the repression of entire chromosomal regions containing genes expressed in a specific cell type, e.g. during the developmental switches leading to different cell lineages [ 25 ]. (biomedcentral.com)
  • These CD34+ cells were isolated using immunomagnetic beads (Miltenyi, #130-046-702) after gradient centrifugation (Axis-Shield PoC AS, Norway). (biomedcentral.com)
  • PDF) Kraan J, van den Broek P, Verhoef C, Grunhagen DJ, Taal W, Gratama JW, Sleijfer SEndothelial CD276 (B7-H3) expression is increased in human malignancies and distinguishes between normal and tumour-derived circulating endothelial cells. (researchgate.net)
  • Results: No difference in antigen expression between normal and malignant endothelial cells (ECs) was found for CD54, CD109, CD137, CD141, CD144 and CXCR7. (researchgate.net)
  • The aim of this study is to investigate the role of activated ovarian endothelial cells in early in-vitro follicular development. (biomedcentral.com)
  • After a constitutive activation of the Akt pathway was initiated, early follicles (50-150 um) were mechanically isolated from 8-day-old mice and co-cultured with these activated ovarian endothelial cells (AOEC) ( n = 32), gel ( n = 24) or within matrigel ( n = 27) in serum free media for 14 days. (biomedcentral.com)
  • Endothelial cells (ECs) may elaborate essential "angiocrine factors" involved in organ regeneration. (biomedcentral.com)
  • Based on review of current literature, this study examined the expression level of 35 genes that are known to be involved in endothelial progenitor cell migration and function in magnetically sorted Lin - /VEGF-R2 + endothelial progenitor cells obtained from the bone marrow of Akita mice in the early stages of diabetes (18 weeks) using RT-PCR and Western blotting. (biomedcentral.com)
  • We observed a significant increase in the number of Lin - /VEGF-R2 + endothelial progenitor cells within the bone marrow in diabetic mice compared with non-diabetic mice. (biomedcentral.com)
  • Low SDF-1 expression may contribute to the dysfunctional mobilization of bone marrow Lin - /VEGF-R2 + endothelial progenitor cells, which may contribute to microvascular injury in early diabetes. (biomedcentral.com)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • CD133 + stem cells were purified from patient-derived sternal bone marrow using the recently developed automatic CliniMACS Prodigy® BM-133 System (Prodigy). (biomedcentral.com)
  • MSCs with a mesodermic origin were first described by Alexander Friedenstein and colleagues in the 1960s and 1970s[ 1 ] as non-hematopoietic bone marrow (BM) cells that adhered to plastic and that could develop into colonies with a fibroblastic appearance. (biomedcentral.com)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • GFP- and by inference Evi1- positive cells were strongly enriched for cells with multilineage and long-term repopulation potential suggesting that was a highly specific and discriminative marker for murine HSCs. (acancerjourney.info)
  • The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
  • CD326 is expressed on the majority of epithelial cells, and is considered a pan-carcinoma antigen. (thermofisher.com)
  • Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
  • Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
  • They were expanded for three times in serum-free X-vivo 15 moderate (Lonza Basel Switzerland) filled with penicillin streptomycin glutamax (Sigma-Aldrich GF 109203X Seelze Germany) and 50 ng/ml of every fms-related tyrosine kinase 3 ligand (FLT3-L) stem cell aspect (SCF) and thrombopoietin (TPO) (Peprotech Hamburg Germany). (acancerjourney.info)
  • The success of allogeneic stem cell transplant is hampered by the development of acute and chronic graft-versus-host disease (GvHD) which has direct impact on treatment-related mortality and morbidity. (atlantis-press.com)
  • Dendritic cells acquire antigen from apoptotic cells and induce class I-restricted CTLs. (nwbio.com)
  • Hence, we established a two-phase expansion protocol with IL-15 to induce an early NK cell expansion, followed by short exposure to IL-21 that boosted the cytotoxic activity of NK cells against RMS cells. (frontiersin.org)
  • The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
  • Large-scale immunomagnetic selection of CD14+ monocytes to generate dendritic cells for cancer immunotherapy: a phase I study. (nwbio.com)
  • To determine if a specific subpopulation of CB-MNC are responsible for these protective activities, we depleted CB-MNC of various cell types and found that only removal of CB CD14 + monocytes abolished neuroprotection. (biorxiv.org)
  • These findings suggest that CD14 + monocytes are a critical cell-type when treating HI with CB-MNC. (biorxiv.org)
  • Right here, we demonstrate that integrin-associated protein (IAP) is really a cell surface area marker ideal for enrichment of hPSC-derived mesDA progenitor cells. (epf2013.org)
  • Producing cells according to Good Manufacturing Practices (GMPs) is a global challenge for the production of all cells for use in humans, specifically mesenchymal stem/stroma cells (MSCs), one of the most promising tools in cellular therapy and regenerative medicine. (biomedcentral.com)
  • The biological activity of automatically isolated CD133 + cells was evaluated and compared to manually isolated CD133 + cells via functional assays as well as immunofluorescence microscopy. (biomedcentral.com)
  • The aim of this study was to identify early diabetes-related changes in the expression of genes that have been reported to be closely involved in endothelial progenitor cell migration and function. (biomedcentral.com)
  • Two genes, SDF-1 and SELE, were significantly differentially expressed in diabetic Lin - /VEGF-R2 + endothelial progenitor cells and six other genes, CAV1, eNOS, CLDN5, NANOG, OCLN and BDNF, showed very low levels of expression in diabetic Lin - /VEGF-R2 + progenitor cells. (biomedcentral.com)
  • Gene expression data from 24 experiments for 8 different cell types of the human myelopoietic lineage were used to generate an integrated myelopoiesis dataset of 9,425 genes, each reliably associated to a unique genomic position and chromosomal coordinate. (biomedcentral.com)
  • Colonies derived from different types of progenitor cells are classified and counted based on morphological and phenotypic criteria. (stemcell.com)
  • Furthermore GFP-positive progenitor cells yielded higher numbers of highly proliferative colonies in GF 109203X single-cell tradition and more colonies in semisolid press than their GFP-negative counterparts (10). (acancerjourney.info)
  • Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
  • The regeneration potential of these cells is mainly based on their direct contribution to neovascularization and on their secretion of various paracrine factors activating pro-angiogenic mechanisms [ 13 - 16 ]. (biomedcentral.com)
  • From this perspective, the aim of this study was to analyze the expression and activation profile of STP involved in the mechanisms of cell proliferation/quiescence and survival of the progenitor CD34+ cells from chronic phase (CP) CML. (oncotarget.com)
  • However human cells have more sophisticated mechanisms than mouse cells to protect them from your action of oncogenes. (acancerjourney.info)
  • Determining which cell types in CB-MNC enhance brain tissue repair, and the mechanisms by which they do so, will optimize decisions on dosing, route of administration, treatment frequency, and other critical clinical and regulatory parameters. (biorxiv.org)
  • Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
  • The term latent reservoir is primarily reserved for latently infected cells which retain the capacity to produce infectious virus particles [ 20 ] and requires assays measuring virus particle production or transcribed HIV mRNA following activation of latently HIV-infected cells [ 19 ]. (biomedcentral.com)
  • Because of their multi/pluripotency and immunosuppressive properties, mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair. (biomedcentral.com)
  • Stromal cells that surround follicles in the secondary stage or later become organized into thecal layers, in which the innermost part contains blood vessels. (biomedcentral.com)
  • A neuronal lineage marker is an endogenous tag that is expressed in different cells along neurogenesis and differentiated cells such as neurons. (wikipedia.org)
  • 2002). Newborn mice were sublethally irradiated (1Gy) within 5 days of birth and 2e10 5 human cord blood-derived CD34+ cells were injected intra-hepatically. (biomedcentral.com)
  • A neuronal lineage marker can be either DNA, mRNA or RNA expressed in a cell of interest. (wikipedia.org)
  • He also described very precisely the purkinje cells, the chick cerebellum and the neuronal circuit of the rodent hippocampus. (wikipedia.org)
  • We also used positively selected subpopulations of CB-MNC and PB-MNC in this assay and demonstrated that purified CB-CD14 + cells, but not CB-PB CD14 + cells, efficiently protected neuronal cells from death and reduced glial activation following OGD. (biorxiv.org)
  • Mononuclear cell (MNC) prepared from human umbilical cord blood (CB) are candidate therapeutics for treating hypoxic-ischemic (HI) brain injuries. (biorxiv.org)
  • An ideal marker is specific to a given cell type in normal conditions and/or during injury. (wikipedia.org)
  • 2010). No organized marker identification research have already been reported for individual mesDA cells. (epf2013.org)
  • Results Id of IAP being a Cell Surface area Marker for mesDA Progenitor Cells To recognize a Prp2 surface area marker ideal for cell sorting, a stream was performed by us cytometry-based surface area marker display screen on hPSC-derived mesDA progenitor cells, generated in line with the Iopanoic acid process produced by Kirkeby et?al. (epf2013.org)
  • Open up in another window Body?1 Id of IAP being a Cell Surface area Marker Expressed in FOXA2+ mesDA Progenitor Cells (A) mesDA had been differentiated based on the process of Kirkeby et?al. (epf2013.org)
  • Cells were harvested for the flow-cytometry-based surface area marker verification on d16 and d11. (epf2013.org)
  • D) Schematic illustration from the gating technique useful for the cell surface area marker screening. (epf2013.org)
  • Very recently using a (marker gene knocked into the locus Kataoka reported the regulatory regions were active almost specifically in hematopoietic stem and progenitor cells (10). (acancerjourney.info)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Relationship between hematogenous tumor cell dissemination and cellular immunity in DCIS patients. (nature.com)
  • Babatz J, Röllig C, Löbel B, Folprecht G, Haack M, Günther H, Köhne CH, Ehninger G, Schmitz M, Bornhäuser M. Induction of cellular immune responses against carcinoembryonic antigen in patients with metastatic tumors after vaccination with altered peptide ligand-loaded dendritic cells. (nwbio.com)
  • Cell separation technologies play a vital role in the graft engineering of hematopoietic cellular fractions, particularly with the rapid expansion of the field of cellular therapeutics. (atlantis-press.com)
  • Cell-generated EV spike-in models were isolated and purified by size-exclusion chromatography, stained with cellular dyes and characterized by nano flow cytometry. (biomedcentral.com)
  • Vaccination with tumor lysate-pulsed dendritic cells elicits antigen-specific, cytotoxic T-cells in patients with malignant glioma. (nwbio.com)
  • In the last years, adoptive transfer of natural killer (NK) cells came into the focus of translational medicine, because of their high cytotoxic potential against transformed malignant cells. (frontiersin.org)
  • With their ability to detect and directly destroy virally infected or malignant cells, natural killer (NK) cells form an important part of the first line defense of the immune system. (frontiersin.org)
  • Cells referred to as MSCs originating from various tissues are now used in clinical trials. (biomedcentral.com)
  • These elaborate signals promoted by ECs have been termed "angiocrine factors" and have been shown to be crucial for the maintenance of organ-specific tissues and tumor cells [ 12 ]. (biomedcentral.com)
  • Therefore, we propose that damage to and subsequent release of mtDNA elicits a protective signalling response that enhances nDNA repair in cells and tissues, suggesting that mtDNA is a genotoxic stress sentinel. (regenerativemedicine.net)
  • Diabetes is known to impair the number and function of endothelial progenitor cells in the circulation, causing structural and functional alterations in the micro- and macro-vasculature. (biomedcentral.com)
  • A main challenge of NK cell therapy is that it requires a high amount of functional NK cells. (frontiersin.org)
  • The discovery of various proteins specific to certain cells led to the production of cell-type-specific antibodies that have been used to identify cells. (wikipedia.org)
  • Magnetic cell sorting continues to be reported to permit quicker and gentler managing of cells (Bosio et?al. (epf2013.org)
  • 2014). Significantly, magnetic cell sorting can be employed in large-scale scientific techniques under sterile circumstances (Despres et?al. (epf2013.org)
  • Mouse ovarian ECs were isolated using magnetic cell sorting or by FACS and cultured in serum free media. (biomedcentral.com)
  • Immature dendritic cells phagocytose apoptotic cells via alphavbeta5 and CD36, and cross-present antigens to cytotoxic T lymphocytes. (nwbio.com)
  • Phase I/II study of vaccination with electrofused allogeneic dendritic cells/autologous tumor-derived cells in patients with stage IV renal cell carcinoma. (nwbio.com)
  • Dendritic cell immunotherapy for patients with metastatic renal cell carcinoma: University of Tokyo experience. (nwbio.com)
  • Banchereau J, Ueno H, Dhodapkar M, Connolly J, Finholt JP, Klechevsky E, Blanck JP, Johnston DA, Palucka AK, Fay J. Immune and clinical outcomes in patients with stage IV melanoma vaccinated with peptide-pulsed dendritic cells derived from CD34+ progenitors and activated with type I interferon. (nwbio.com)
  • Maturation and trafficking of monocyte-derived dendritic cells in monkeys: implications for dendritic cell-based vaccines. (nwbio.com)
  • Vaccination of prostatectomized prostate cancer patients in biochemical relapse, with autologous dendritic cells pulsed with recombinant human PSA. (nwbio.com)
  • Bleumer I, Tiemessen DM, Oosterwijk-Wakka JC, Völler MC, De Weijer K, Mulders PF, Oosterwijk E. Preliminary analysis of patients with progressive renal cell carcinoma vaccinated with CA9-peptide-pulsed mature dendritic cells. (nwbio.com)
  • White blood cell, eosinophil, CD4- and CD8-positive lymphocyte counts, and serum interleukin-5 (IL-5) and IgE levels were determined before, 2 and 4 weeks after switching to vitamin E-bonded dialyzers. (dadamo.com)
  • As a result, T cell depletion through positive selection of CD34+ cells has emerged as a promising. (atlantis-press.com)
  • The primary purpose of this study is to determine whether breast cancer tumors respond (as measured by pathologic complete response: the absence of microscopic evidence of invasive tumor cells in the breast) to combined chemotherapy of AC(doxorubicin and cyclophosphamide) followed by paclitaxel plus trastuzumab or lapatinib or both given before surgery to patients with HER2-positive breast cancer. (stanford.edu)
  • After 6 passages, flow cytometry showed 93% of cells grown in serum-free culture were VE-cadherin positive, CD-31 positive and CD 45 negative, matching the known EC profile. (biomedcentral.com)
  • Thus, these methodologies are extremely useful to obtain large batches of fairly pure EV samples from cell cultures and enable unbiased characterization of EV subpopulations. (biomedcentral.com)
  • CD133 + stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. (biomedcentral.com)
  • On average, 0.88 × 10 6 viable CD133 + cells with a mean log 10 depletion of 3.23 ± 0.19 of non-target cells were isolated. (biomedcentral.com)
  • According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
  • The cells were first named colony-forming unit-fibroblasts (CFU-Fs). (biomedcentral.com)
  • In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)
  • In sum, we report that CD34+ cells from CP-CML are characterized by a proteomic and phospho-proteomic profile that promotes quiescence through the inhibition of proliferation and the promotion of survival. (oncotarget.com)
  • Dormant cancer cells: programmed quiescence, senescence, or both? (nature.com)
  • Because all cells constantly generate and recycle EVs, they provide accurate timed snapshots of individual pathophysiological status. (biomedcentral.com)
  • Using this technique, it is possible to locate gene expression to specific cell types in specific regions and observe how changes in this distribution occur throughout the development and correlate with the behavioral manipulations. (wikipedia.org)
  • While BM-MSCs have high cell-to-cell heterogeneity in gene expression, the cell subtypes that contribute to this heterogeneity in vivo in humans have not been characterized. (ijbs.com)
  • This differential signaling activation network may be addressed by novel targeted therapies aimed at eradicating CML stem cells. (oncotarget.com)
  • CD4 + T cells contribute to the remodeling of the microenvironment required for sustained tumor regression upon oncogene inactivation. (nature.com)