Progenitor cells cells expressing pax7 pax7 transcription factor. Medical search. Frequent questions

The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)
CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)
When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
The latest advances in the field of spinal cord development and its regeneration following damage were discussed at a recent EMBO workshop 'Spinal cord development and regeneration' in Sitges, Spain (October, 2014), highlighting the use of direct visualization of cellular processes, genome-wide molecular techniques and the development of methods for directed stem cell differentiation and regeneration. (biologists.com)
Growth is regulated by gene expression variation at different developmental stages of biological processes such as cell differentiation, disease progression, or drug response. (bvsalud.org)
Afterwards, we have comparatively evaluated the hematopoietic and erythroid as well as neural differentiation potential of human cord blood and neural stem cell derived iPS cells (Hargus et al. (ruhr-uni-bochum.de)
Patient-derived induced pluripotent stem cells and their differentiation derivatives hold great promise as novel in vitro model systems to study human pathogenesis. (ruhr-uni-bochum.de)
The integrin CD49a marks highly cytotoxic epidermal-tissue-resident memory (TRM) cells, but their differentiation from circulating populations remains poorly defined. (scilifelab.se)
Together, our results indicate that combined RUNX2 and RUNX3 activity promotes the differentiation of cytotoxic CD8+CD103+CD49a+ TRM cells, providing immunosurveillance of infected and malignant cells. (scilifelab.se)
For example, these manuscripts establish that elevated Pax7 levels drive quiescence and low Pax7 levels correlate with differentiation. (sciety.org)
mal, T cell differentiation pro. (gsea-msigdb.org)
Keratinocyte proliferation, differentiation, and programmed cell death are complex and carefully choreographed processes. (3hbiomedical.com)

They then differentiate, migrate towards the surface of epidermis, and eventually withdraw from the cell cycle permanently. (3hbiomedical.com)

A lately developed and validated immunotagging technique makes use of three transcription issue antibodies to concurrently isolate enriched neuronal (NeuN+), astrocyte (paired field protein 6 (PAX6)+NeuN-), and oligodendrocyte progenitor ( OLIG2 +NeuN-) nuclei populations from non-diseased, recent (unfixed) snap-frozen postmortem human temporal neocortex tissue. (aetox.com)
Pax6 exerts regional control of cortical progenitor proliferation via direct repression of Cdk6 and hypophosphorylation of pRb. (ox.ac.uk)
The mechanisms by which early spatiotemporal expression patterns of transcription factors such as Pax6 regulate cortical progenitors in a region-specific manner are poorly understood. (ox.ac.uk)
Pax6 is expressed in a gradient across the developing cortex and is essential for normal corticogenesis. (ox.ac.uk)
We found that constitutive or conditional loss of Pax6 increases cortical progenitor proliferation by amounts that vary regionally with normal Pax6 levels. (ox.ac.uk)
We compared the gene expression profiles of equivalent Pax6-expressing progenitors isolated from Pax6⁺/⁺ and Pax6⁻/⁻ cortices and identified many negatively regulated cell-cycle genes, including Cyclins and Cdks. (ox.ac.uk)
Cyclin/Cdk repression inhibits retinoblastoma protein (pRb) phosphorylation, thereby limiting the transcription of genes that directly promote the mechanics of the cell cycle, and we found that Pax6 inhibits pRb phosphorylation and represses genes involved in DNA replication. (ox.ac.uk)
Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling. (uiowa.edu)

Fluorescence in situ hybridization (FISH) was carried out on FFPE sections with FOXO1/PAX3 and/or FOXO1/PAX7 probes. (aetox.com)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
PAX3 and PAX7 binding protein 1. (gsea-msigdb.org)

WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
In both processes, rapid proliferation gives rise to new tissue, cell fate has to be specified within that tissue, and distinct positional identities have to be established to generate a properly patterned structure. (frontiersin.org)
Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)

Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)
Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)
There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)
The identification of heterogenous distribution of factors across the myofiber is an important contribution for dissecting how muscle stem cell diversity in a tissue is achieved. (sciety.org)

The quiescent muscle stem cell (QSC) pool is heterogeneous and generally characterized by the presence and levels of intrinsic myogenic transcription factors. (sciety.org)
The control of MuSC state depends on the expression levels of myogenic transcription factors. (biomed.news)
Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)

These networks consist of transcription factors and their associations with genes they regulate. (bvsalud.org)

Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)

The protocol focuses on the genome-wide prediction of regulatory interactions between transcription factors and genes, leveraging publicly available gene expression data in conjunction with well-established benchmarks. (bvsalud.org)
Over the past decade, gene expression data has been widely used for reconstructing dGRN by computing correlations between the differentially expressed genes (DEGs). (bvsalud.org)
[ 4 ] The Hox genes, well described as the master regulators of development, encode a set of transcription factors that specify the identity of particular segments during embryogenesis. (medscape.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
human blood monocytes were isolated, activated and harvested at several timepoints In this study, we identified genes that were differentially expressed in human monocytes activated with eiter NOD2L and/or TLR2/1L. (gsea-msigdb.org)

Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
The meeting, organized by Elisa Martí (IBMB-CSIC, Barcelona, Spain), Jonas Muhr (Karolinska Institutet, Stockholm, Sweden) and Kate Storey (University of Dundee, UK), brought together international researchers interested in spinal cord development and those who are developing strategies to restore the function of the spinal cord in pathological situations using regeneration of the damaged tissue, including controlled cell replacement. (biologists.com)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)

Nevertheless, efforts to equally isolate astroglia from the non-neuronal (NeuN-) factor are missing. (aetox.com)

The sonic hedgehog transcription pathway has also been linked to the formation of specific kinds of cancerous tumors, including the embryonic cerebellar tumor and medulloblastoma, as well as the progression of prostate cancer tumours. (wikipedia.org)
Two extra OLIG2 optimistic circumstances confirmed inconclusive FISH outcomes, however have been optimistic for TFAP2B and ALK, what means that these tumours expressed fusion optimistic signature. (aetox.com)
In addition, mtDNA stress in TFAM-deficient mouse melanoma cells produces tumours that are more resistant to doxorubicin in vivo. (regenerativemedicine.net)

10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
Eph continues to be expressed in the larval CNS and imaginal discs, as well as pupal and adult stages as assayed by Northern blot analysis. (sdbonline.org)

Protein expression of OLIG2 , a novel marker in RMS, was investigated utilizing antibody EP112 (Cell Marque). (aetox.com)
We demonstrate enrichment of RUNT family transcription-factor-binding motifs in human epidermal CD8+CD103+CD49a+ TRM cells, paralleled by high RUNX2 and RUNX3 protein expression. (scilifelab.se)

For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

Long-term survival of dopamine neurons derived from parthenogenetic primate embryonic stem cells (cyno-1) after transplantation. (uiowa.edu)

Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)

The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)
Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)

Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
In vitro stimulation of circulating CD8+CD45RA-CD62L+ T cells with IL-15 and TGF-β induced CD49a expression and cytotoxic transcriptional profiles in a RUNX2- and RUNX3-dependent manner. (scilifelab.se)

We are further developing our iSTEM (Molecular and Developmental Stem Cell Biology) master program since its inception in 2010 by incorporating state-of-the-art topics of stem cell research in the teaching modules. (ruhr-uni-bochum.de)

Conditional expression of Otx2 in these morphant embryos cell-autonomously rescues the formation of the ZLI at its correct location. (silverchair.com)

Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)

Sequencing of paired skin and blood samples revealed clonal overlap between epidermal CD8+CD103+CD49a+ TRM cells and circulating memory CD8+CD45RA-CD62L+ T cells. (scilifelab.se)

Dendritic cells play central roles in innate and adaptive immunity that sense the presence of microbes/ foreign antigens and initiate innate immune response by capturing microbial proteins (antigens), process and presenting to T cells and to initiate foreign antigen specific adaptive immune response. (3hbiomedical.com)

Despite its cellular simplicity (five pairs of neurons), the MG exhibits conservation of transcription factor expression with the spinal cord of vertebrates. (biologists.com)

Somatic cells can be reprogrammed to a pluripotent embryonic stem cell-like state (induced pluripotent stem (iPS) cells) by combinations of the transcription factors OCT4, SOX2, NANOG, LIN28, KLF4 and cMYC (Takahashi et al. (ruhr-uni-bochum.de)
Genetic selection of sox1GFP-expressing neural precursors removes residual tumorigenic pluripotent stem cells and attenuates tumor formation after transplantation. (uiowa.edu)

Identification of embryonic stem cell-derived midbrain dopaminergic neurons for engraftment. (uiowa.edu)
To further define which neurons express Eph, antibodies were generated to the cytoplasmic portion of the Eph protein. (sdbonline.org)
Based upon the numbers and morphology of the staining axons, Eph is expressed by a large subset of interneurons and does not appear to be expressed by motor neurons (Scully, 1999). (sdbonline.org)

This method was proven to be helpful for the characterization of cell type-specific transcriptome alterations in main pathological epilepsy neocortex. (aetox.com)

To this end, this article briefly discusses numerous important processes in head and neck embryology, namely the implications of patterning in hindbrain development, the diverse roles of neural crest cells, migration of the neural crest cells into the branchial arches (particularly the hyoid arch), and the genetic control of these processes. (medscape.com)
The development of the hindbrain (rhombencephalon) and the subsequent delamination of the neural crest cells are interrelated processes that need to be understood to appreciate the development of the branchiomotor cranial nerves in general and the facial nerve in particular. (medscape.com)

Evaluation of NKX2-2 expression in round cell sarcomas and other tumors with EWSR1 rearrangement: imperfect specificity for Ewing sarcoma. (nih.gov)
Gene regulatory network is the architecture of transcription factors (TFs) and their gene targets, which help in controlling their expression as required by a phenotype during various environmental perturbations. (bvsalud.org)
We were able to demonstrate, that mouse and human neural stem cells which express SOX2, KLF4 and cMYC, can be reprogrammed to iPS cells by retroviral expression of OCT4 (Kim, Zaehres et al. (ruhr-uni-bochum.de)
Human skin-resident CD8+ T cells require RUNX2 and RUNX3 for induction of cytotoxicity and expression of the integrin CD49a. (scilifelab.se)
One significant issue with the manuscript is the "discovery" of an SC continuum related to the relative levels of Pax7 expression. (sciety.org)
In the original manuscript we acknowledged that SCs exist on a continuum-a gradual transition from one state to another, based on scRNA-seq studies and the present data (Dll4, Pax7 and Ddx6 expression). (sciety.org)
As a transcription factor whose expression is increased by DNA damage, p53 blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
EphA1 mis-expression did not cause neurodegeneration, shorten lifespan or affect memory but flies mis-expressing the wild-type or mutant receptor were hyper-aroused, had reduced sleep , a stronger circadian rhythm and increased clock neuron activity and excitability. (sdbonline.org)

Cell stem cell 28.2 (2021 Feb 4): 343-355.e5. (uiowa.edu)
The premise behind this manuscript is important and timely for muscle biologists and for stem cell biologists. (sciety.org)

Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)
In cultured primary fibroblasts and cancer cells, the chemotherapeutic drug doxorubicin causes mtDNA damage and release, which leads to cGAS STING dependent ISG activation. (regenerativemedicine.net)

Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
Our data shows that a post-translational mechanism controls spatial heterogeneity of Notch ligands in a multinucleated niche cell to maintain a continuum of diverse states within the SC pool during tissue homeostasis. (sciety.org)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Apart from their protective functions, keratinocytes express adhesion molecules and cytokines, further suggesting an implication in skin innate immunity, tissue homeostasis, wound healing, cancer development, and skin-based gene-therapy. (3hbiomedical.com)

In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)
Another stimulus that activates satellite cells is stretching of the muscle. (arvojournals.org)
7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)

Usefulness of NKX2.2 Immunohistochemistry for Distinguishing Ewing Sarcoma from Other Sinonasal Small Round Blue Cell Tumors. (nih.gov)

In melanoma patients, high RUNX2, but not RUNX3, transcription correlated with a cytotoxic CD8+CD103+CD49a+ TRM cell signature and improved patient survival. (scilifelab.se)

Rapidly expanding technological innovations have led to a resurgence of interest in defining the factors that enable regenerative healing, and in coupling these factors to human therapeutic interventions. (frontiersin.org)

Human Peripheral Blood Plasmacytoid Dendritic Cells (Human PDCs) are isolated from human PBMCs of a single donor. (3hbiomedical.com)

Specifically, SHH attracts retinal ganglion cell (RGC) axons at low concentrations and repels them at higher concentrations. (wikipedia.org)

Our results indicate that Pax6's modulation of cortical progenitor cell cycles is regional and direct. (ox.ac.uk)

We are currently focusing to model neural (Frontotemporal Dementia) and skeletal muscle (Duchenne, LGMD) diseases with human iPS cells and CRISPR/Cas9 genome editing. (ruhr-uni-bochum.de)
Muscle samples were collected and myogenic cells were isolated and cultivated. (nature.com)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

At the cellular level, several processes are highly controlled, including the establishment and maintenance of apico-basal polarity, the control of the cell cycle and cell cycle exit, and the acquisition of cell subtype identities. (biologists.com)

Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)

For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)

These changes can be inherited and are, therefore, found in every cell, but more often, they are somatically acquired and restricted to tumor cells. (medscape.com)

Sonic hedgehog contributes to cell growth, cell specification and formation, structuring and organization of the body plan. (wikipedia.org)

The encoded protein is likely to be a nuclear transcription factor. (nih.gov)

Anatomy39

Organisms9

Diseases2

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes64

Disciplines and Occupations1

Information Science3

Differentiation13

Withdraw from the cell cycle1

PAX68

PAX33

Proliferation8

Myofiber6

Myogenic transcription factors3

Regulate1

Skeletal muscle regeneration1

Genes7

Regeneration6

Neuronal1

Tumours3

Adult3

Protein expression2

Located beneath the basal lamina1

Embryonic stem1

Myonuclei1

Progression2

Vitro2

Biology1

Conditional1

Quiescent muscle1

Epidermal1

Proteins1

Spinal cord1

Pluripotent2

Neurons3

Characterization1

Neural crest2

Expression8

Stem Cell2

Activation2

Tissue5

Satellite cells4

Immunohistochemistry1

Melanoma1

Regenerative1

Donor1

Axons1

Results indicate1

Muscle3

Processes1

Regulatory1

Cycle3

Tumor1

Contributes1

Nuclear1