AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceNamed GroupsHealth Care
Hematopoietic Stem CellsStem CellsHematopoietic Stem Cell TransplantationCell DifferentiationAntigens, CD34HematopoiesisCell LineageBone Marrow CellsColony-Forming Units AssayHematopoietic Stem Cell MobilizationMyeloid Progenitor CellsStem Cell TransplantationCells, CulturedMice, Inbred C57BLCell ProliferationNeural Stem CellsAdult Stem CellsBone MarrowMultipotent Stem CellsFlow CytometryStem Cell NicheFetal BloodStem Cell FactorCell SeparationTransplantation, HomologousNeoplastic Stem CellsLymphoid Progenitor CellsGranulocyte Colony-Stimulating FactorErythroid Precursor CellsMice, TransgenicEndothelial CellsProto-Oncogene Proteins c-kitBone Marrow TransplantationFetal Stem CellsAntigens, CDCell DivisionCell CountMice, SCIDMesenchymal Stromal CellsHematopoietic SystemMice, KnockoutGene Expression Regulation, DevelopmentalChemokine CXCL12RegenerationCell MovementSignal TransductionTransplantation, AutologousGranulocytesNeurogenesisInterleukin-3Cell Culture TechniquesNestinMice, Inbred NODGraft vs Host DiseaseLymphopoiesisHomeodomain ProteinsPlant StemsTransplantation ConditioningMesonephrosTime FactorsPluripotent Stem CellsMegakaryocytesTransduction, GeneticRetroviridaeStromal CellsEmbryo, MammalianCell SurvivalBlood CellsMyelopoiesisGraft SurvivalLentivirusPhenotypeGreen Fluorescent ProteinsErythropoiesisThrombopoietinHematopoietic Cell Growth FactorsHematologic NeoplasmsReverse Transcriptase Polymerase Chain ReactionGranulocyte-Macrophage Progenitor CellsTranscription FactorsBasic Helix-Loop-Helix Transcription FactorsNeuronsHematopoiesis, ExtramedullaryGenetic VectorsOligodendrogliaBlood Cell CountTransplantation ChimeraBiological MarkersNeovascularization, PhysiologicLeukapheresisCoculture TechniquesCord Blood Stem Cell TransplantationCell CycleLeukemiaReceptors, CXCR4Antigens, LyWhole-Body IrradiationGene Expression RegulationEmbryonic Stem CellsAntigens, CD38
DifferentiationTransplantationHSCsHSPCCD34LineagesMyeloid progeMesenchymal StemGive rise to progenitor cellsBoneTissuesCord bloodPrecursorsHematopoiesisBiologyFetalMultipotent progenitorsFunctionalDifferentiateHeterogeneityCharacterizationVivoGeneVitroEmbryonicLymphocytesClinicalCaudal hematopoieticPluripotent stemAutologous2022PrecursorHumansEndothelial cellsGeneticImmuneFlow CytometryEarly lymphoidMiceBloodRegenerative MedicineGenomeSubpopulationAllogeneicMegakaryocyteSelf-renewalAcute myeloidFunctionallyClustersPopulations
Differentiation28
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
  • Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
  • erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
  • In hematopoietic tissues, a group of ECs are highly specialized and constitute the vascular HSPC niche to support HSPC maturation, expansion, and differentiation. (go.jp)
  • The team visualized endothelial differentiation step-by-step from the endoderm to the HSPC niche-constituting ECs via islet1+ endothelial progenitors by live imaging-based lineage tracing. (go.jp)
  • Progenitor cells, also known as precursor cells, are a type of cell that falls somewhere between stem cells and fully differentiated, specialized cells in terms of their differentiation potential. (thenoveldifference.com)
  • They have already committed to a particular differentiation pathway, meaning they are destined to become a specific type of cell, such as a red blood cell, white blood cell, or platelet in the case of hematopoietic progenitor cells. (thenoveldifference.com)
  • Negative regulatory factors inhibit the proliferation of haematopoietic stem cells thus protecting them from differentiation pressures. (st-andrews.ac.uk)
  • Features and Radiosensitivity of HSPCs Compact disc34+ HSPCs had been cultured in serum-free liquid moderate as referred to in the Components and Solutions to investigate the consequences of X-irradiation for the cell proliferation and differentiation. (hiv-proteases.com)
  • Self-renewal and differentiation are tightly controlled to maintain haematopoietic stem cell (HSC) homeostasis in the adult bone marrow 1,2 . (elsevierpure.com)
  • During fetal development, expansion of HSCs (self-renewal) and production of differentiated haematopoietic cells (differentiation) are both required to sustain the haematopoietic system for body growth 3,4 . (elsevierpure.com)
  • These cells evolve from the differentiation of neural progenitor cells (NPCs). (differencebetween.com)
  • They play a role in cell development and differentiation. (differencebetween.com)
  • Hematopoiesis is an important model for stem cell differentiation with great medical significance. (europa.eu)
  • During vertebrate embryogenesis , fetal hematopoietic stem and progenitor cells (HSPCs) exhibit expansion and differentiation properties in a supportive hematopoietic niche. (bvsalud.org)
  • Early erythroid progenitors are found at a quite low frequency relative to later stages of erythroid differentiation, such as the pro-erythroblast and the basophilic erythroblast stages which can be detected by flowcytometry directly ex-vivo. (wikipedia.org)
  • By day 2 from the time of plating, each CFU-E colony will contain between 8 (minimum) and 64 hemoglobinized cells most of which are in their end-stage of erythroid differentiation. (wikipedia.org)
  • Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
  • This includes the analysis of genes in germline stem cell proliferation and differentiation. (ncl.ac.uk)
  • Single-cell transcriptomics revealed that rostro-caudal organization was already established at 24 h of differentiation, and that the first markers of a neural-specific transcription program emerged in the rostral cells at 48 h. (nature.com)
  • Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
  • Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
  • and positive regulation of cell differentiation. (mcw.edu)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • These malignancies are further characterized by the maturity and differentiation of the individual cell types and are divided into acute leukemias such as acute myeloid leukemia and acute lymphoblastic leukemia and chronic leukemias such as chronic myeloid leukemia and chronic lymphocytic leukemia. (medscape.com)
  • Different characteristics of the nuclei and cytoplasm of the cell allow differentiation by instrumentation and microscopy. (medscape.com)
Transplantation18
  • HSCs specifically are defined by their ability for self-renewal and to reconstitute the entire hematopoietic system following transplantation. (stemcell.com)
  • It is used both by hematopoietic researchers and clinical labs to assess the potency of transplantation units of cord blood (CB) and hematopoietic cellular therapy products. (stemcell.com)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
  • At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
  • Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
  • Hematopoietic stem cells are widely used in medical treatments, particularly in bone marrow transplantation for individuals with hematological disorders like leukemia or aplastic anemia. (thenoveldifference.com)
  • Both cells have common applications in various cell-based therapies such as tissue regeneration and transplantation. (differencebetween.com)
  • Together, both IST and stem cell transplantation are reasonable treatment options for patients with AA. (springermedizin.at)
  • Transplantation of CD31 + Lin - c-kit - cells fails to protect lethally irradiated recipients, while CD31 + Lin - c-kit + Sca-1 - cells (CD31 + Sca-1 - ) provide radioprotection in the absence of long-term donor-derived hematopoiesis. (elsevierpure.com)
  • Although most clinicians would agree that hematopoietic progenitor cell transplantation after reinduction therapy is frontline therapy for these patients, there is no consensus as to what type of hematopoietic progenitor cell transplantation promises the best event-free and overall survival. (jnccn.org)
  • 1 - 3 However, those with relapsed and/or refractory (R/R) disease have had a historically dismal OS of ≤30% despite reinduction therapy and autologous hematopoietic progenitor cell transplantation (autoHCT) ( Figure 1 ). (jnccn.org)
  • For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
  • I have expertise in novel therapies for acute myeloid leukemia, stem cell mobilization and homing, as well as stem cell transplantation. (rochester.edu)
  • MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
  • Understanding how HSPCs are regulated is of central importance for several areas in clinical hematology, including stem cell transplantation and the treatment of leukemias. (lu.se)
  • The growing need for haematopoietic stem cell transplantation (HSCT) is reflected in the increasing number of transplants performed globally each year. (bvsalud.org)
  • 1 This includes human cells for transplantation such as haematopoietic stem cells from bone marrow, peripheral blood or cord blood. (who.int)
HSCs27
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
  • To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
  • Hypoxia lessened the cellular stress response in both progenitors and HSCs, but the mitigation was more apparent in the HSC population (Fig. C, grey plots), and decreased apoptosis was observed only within the HSC-enriched cluster 5 (Fig. D). These findings are consistent with several reports indicating that HSCs are more vulnerable to strong ER stress than downstream progenitors due to their lower protein folding capacity. (confex.com)
  • Second, compared to BM, extramedullary hematopoietic stem cells/multipotent progenitors (HSCs/MPPs) from the spleen, PB, and mobilized PB to have a shared transcriptional profile and a higher abundance of lineage-primed subsets. (physiciansweekly.com)
  • Hematopoietic stem cells (HSCs) reside in association with bone marrow (BM) sinusoidal vessels in vivo, but the function of BM endothelial cells (ECs) in regulating hematopoiesis is unclear. (duke.edu)
  • Hematopoietic stem cells (HSCs) are a type of multipotent stem cell found primarily in the bone marrow and, to a lesser extent, in peripheral blood. (thenoveldifference.com)
  • HSCs have the remarkable ability to differentiate into a wide range of specialized blood cells. (thenoveldifference.com)
  • Here we used in vivo genetic tracing in mice to analyse the formation of HSCs and progenitors from intra-arterial haematopoietic clusters, which contain HSC precursors and express the transcription factor hepatic leukaemia factor (HLF). (elsevierpure.com)
  • Through kinetic study, we observed the simultaneous formation of HSCs and defined progenitors-previously regarded as descendants of HSCs 5 -from the HLF + precursor population, followed by prompt formation of the hierarchical haematopoietic population structure in the fetal liver in an HSC-independent manner. (elsevierpure.com)
  • The transcription factor EVI1 is heterogeneously expressed within the precursor population, with EVI1 hi cells being predominantly localized to intra-embryonic arteries and preferentially giving rise to HSCs. (elsevierpure.com)
  • These data suggest that fetal HSCs minimally contribute to the generation of progenitors and functional blood cells before birth. (elsevierpure.com)
  • However CB transplants suffer from limited progenitor cell dose leading to delayed neutrophil engraftment and increased mortality (3 4 Recent studies in immunodeficient mice have confirmed the presence of human CB-derived long-term-repopulating hematopoietic stem cells (LT-HSCs) capable of regenerating the lifelong production of all mature blood cells (5). (gasyblog.com)
  • These LT-HSCs show a delayed engraftment pattern in opposition to short-term HSCs (ST-HSCs) that produce short-lived progenitors responsible for the production of mature blood cells and prompt neutrophil recovery (3 5 Hence there is great interest in the development of conditions for robustly expanding these progenitor cells while maintaining or expanding LT-HSCs. (gasyblog.com)
  • mobilized peripheral bloodstream (mPB) cells that are enriched in LT-HSCs (10) (fig. S1 B) and A. Seven hits had been determined after excluding the autofluorescent substances (Fig. 1A and fig. S1C) five which had been known [four (11 12 or previously unidentified (one UM125454 fig. S2) suppressors from the AhR pathway (Fig. 1B). (gasyblog.com)
  • cells (10) (Fig. 1D and fig. S3 B) and A. UM729 didn't broaden mouse HSCs (fig. S4). (gasyblog.com)
  • Hematopoietic stem cells (HSCs) are immature cells found in the peripheral blood and bone marrow. (differencebetween.com)
  • HSCs develop into different types of blood cells in lines called myeloid and lymphoid , which are involved in dendritic cells formation. (differencebetween.com)
  • Here, a combination of different cell surface markers is used to separate the rare HSCs. (differencebetween.com)
  • LVs efficiently transduce slowly dividing cells, including hematopoietic stem-progenitor cells (HSCs), resulting in stable gene transfer and expression. (johnshopkins.edu)
  • Using a single LV containing two constitutive promoters, we achieved strong and sustained expression of both transgenes in transduced engrafting CD34 + HSCs and their progeny, as well as in other human cell types. (johnshopkins.edu)
  • Hematopoietic stem cells (HSCs) maintain quiescence by activating specific metabolic pathways, including glycolysis. (elsevierpure.com)
  • Analysis of phenotypically defined hematopoietic stem cells (HSCs) from the yolk sac, fetal liver, and adult bone marrow demonstrates CD31 expression on these cells throughout development. (elsevierpure.com)
  • The group are currently focused on the pivotal role that HOXA cluster genes play in the demarcation and possible emergence of haematopoietic stem cells (HSCs) and their role in cancer. (nottingham.ac.uk)
  • However, eradication of the leukaemia repopulating cell or stem cell whilst retaining normal HSCs remains a challenge with current experimental therapies. (nottingham.ac.uk)
  • The rapid advancement in understanding the biology of hematopoietic stem cells (HSCs) and cancer stem cells (CSCs) in recent years has renewed interest in investigating the role of stem cells in benzene -induced malignancy and bone marrow depression. (cdc.gov)
HSPC11
  • Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
  • Rare hematopoietic stem and progenitor cell (HSPC) pools outside the bone marrow (BM) contribute to blood formation under stress and illness, but their functions are unknown. (physiciansweekly.com)
  • Researchers compared spleen, PB, and mobilized PB to BM utilizing single-cell RNA-sequencing and/or functional tests to characterize human extramedullary HSPC compartments thoroughly. (physiciansweekly.com)
  • Hematopoietic stem/progenitor cells (HSPC) are known to be functionally diverse in terms of their self-renewal potential and lineage output. (lu.se)
  • They found that islet1 -expressing cells are the progenitors of the venous ECs that constitute the majority of the HSPC niche (Figure 1A). (go.jp)
  • The present results clearly demonstrate that ECs originating from the endoderm via islet1 + endothelial progenitors play a specialized role in forming a functional vascular HSPC niche (Figure 1C). (go.jp)
  • Importantly, we identified an endothelial cell -specific G protein -coupled receptor 182, followed by in vivo and in vitro functional validation of its evolutionally conserved role in supporting HSPC expansion in zebrafish and mice . (bvsalud.org)
  • These findings enhance our understanding of the regulatory mechanism underlying hematopoietic niche for HSPC expansion in vivo and provide insights into improving protocols for HSPC expansion in vitro . (bvsalud.org)
  • In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
  • Here, we report a critical role for the p38MAPK family isoform p38α in initiating hematopoietic stem and progenitor cell (HSPC) proliferation during stress hematopoiesis in mice. (elsevierpure.com)
  • In a second line, we use natural genetic variation to find genes that regulate hematopoietic stem and progenitor cells (HSPC) in humans. (lu.se)
CD3412
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • Culture conditions of CD34(+) cells - either with or without mesenchymal stromal cells (MSCs) - had relatively little impact on DNAm, although proliferation is greatly increased by stromal support. (nih.gov)
  • We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)
  • CD34 + cells after six days in culture were stimulated with atorvastatin (AT), acetylsalicylic acid (ASA), sulforaphane (SR), resveratrol (RV), or metformin (Met) for 48 h. (hindawi.com)
  • EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
  • In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
  • This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
  • While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
  • The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
  • Control (dimethyl sulfoxide DMSO) fed-batch cultures contained mostly differentiated cells (Fig. 2A DMSO) and a reduced frequency of CD34+CD45RA? (gasyblog.com)
  • Here, we performed global microarray-based gene expression measurements on human CD34 + hematopoietic stem and progenitor cells that underwent editing. (biu.ac.il)
  • Colony Forming Cell (CFU) Assay CD34+ cells were selected from HPDSCs with a human CD34 positive selection kit and isolated using automated cell separator RoboSep (StemCell Technologies, Inc., Vancouver, Canada). (ebat2018.org)
Lineages8
  • Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)
  • HSPCs are multipotent cells that can self-renew and differentiate to give rise to all blood cell lineages. (go.jp)
  • Hematopoietic stem cells give rise to progenitor cells, which, in turn, differentiate into specific blood cell lineages. (thenoveldifference.com)
  • Hematopoietic stem/progenitor cells (HSPCs), which can be found in little numbers in hematopoietic tissues, can differentiate into most hematopoietic lineages and self-renew to keep up their undifferentiated phenotype. (hiv-proteases.com)
  • We will pursue the following aims: (i) Generate a refined model of hematopoiesis by comprehensive single-cell RNA-Seq profiling of hematopoietic progenitors, (ii) validate the predicted model by in vivo functional developmental assays and then (iii) test candidate transcription and chromatin factors uncovered by our model for their role in controlling progression towards various lineages using single-cell measurements combined with CRISPR screens. (europa.eu)
  • Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)
  • This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
Myeloid proge1
Mesenchymal Stem2
  • Among the remaining cells, several different cell types are recognized, including hematopoietic stem cells, mesenchymal stem cells (MSCs), megakaryocytic precursors, and endothelial progenitors. (ebat2018.org)
  • In preclinical and clinical studies, progenitor cell therapy (cord blood and mesenchymal stem cells) has shown promise in reversing the underlying pathology of SNHL, the loss of cochlear sensory hair cells. (intechopen.com)
Give rise to progenitor cells1
  • Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
Bone20
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
  • Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
  • The bone marrow serves as the primary residence for hematopoietic stem cells. (thenoveldifference.com)
  • For example, hematopoietic progenitor cells are found in the bone marrow, as this is where blood cell production occurs. (thenoveldifference.com)
  • In addition, ACE resistant AcSDKP analogue (AcSDPψKP) when incubated with bone marrow cells in the absence of captopril also reduced the proportion of GM-CFC in S-phase. (st-andrews.ac.uk)
  • Haematopoietic stem cells were induced into cell cycle by in vivo administration of either 2 Gy-γ-irradiation or the two cytotoxic drugs, cytosine arabinoside (Ara-C) (100 mg/kg i.p.) or 5 flourouracil (5 FU) (150 mg/kg i.v). Bone marrow cells were sampled and incubated in vitro for up to 24 hours. (st-andrews.ac.uk)
  • Incubation alone reduced the proportion of HPP-CFC-1 in S-phase in cytotoxic drug treated bone marrow cells. (st-andrews.ac.uk)
  • When cultures, which were incubated with captopril, were assayed for AcSDKP levels, captopril induced an increase in AcSDKP levels in both control normal bone marrow cells and cells derived from Ara-C treated mice. (st-andrews.ac.uk)
  • However, it did not affect AcSDKP levels in cultures derived from 5 FU and 2 Gy treated mice, AcSDKP together with captopril were shown to inhibit S-phase cell entry of HPP-CFC-1 when they were incubated with bone marrow cells derived from mice treated with either 2 Gy-γ-irradiation or cytotoxic drug insults. (st-andrews.ac.uk)
  • CFU-E colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue (bone marrow, spleen, or fetal liver), which may be reflective of the organism's demand for oxygen delivery to the tissues or a hematopoietic disorder. (wikipedia.org)
  • Further evaluation of the CD31 + lineage marker-negative fraction of adult bone marrow reveals functionally distinct cell subsets. (elsevierpure.com)
  • Although donor-derived leukocytes were not detected in CD31 + Sca-1 - recipients, donor-derived erythroid cells were transiently produced during the initial phases of bone marrow recovery. (elsevierpure.com)
  • As the affected cell lines continue to divide and to provide the marrow with dysplastic cells, bone marrow dysfunction becomes apparent. (medscape.com)
  • It is arise from hematopoietic stem and progenitor cells in the bone marrow. (medgadget.com)
  • The interplay between benzene and stem cells is complex involving the stem cell, progenitor, and HSC niche compartments of the bone marrow. (cdc.gov)
  • In this prospect, benzene metabolites formed through metabolism in the liver and bone marrow cause damage in hematopoietic cells via multiple mechanisms that, in addition to traditionally recognized chromosomal aberration and covalent binding, incorporate oxidative stress, alteration of gene expression, apoptosis, error-prone DNA repair, epigenetic regulation, and disruption of tumor surveillance. (cdc.gov)
  • Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
  • Decreased WBC count, leukopenia, is seen when supply is depleted by infection or treatment such as chemotherapy or radiation therapy, or when a hematopoietic stem cell abnormality does not allow normal growth/maturation within the bone marrow, such as myelodysplastic syndrome or leukemia. (medscape.com)
  • These are the malignant proliferations of abnormal clones of white blood cells within the bone marrow that are broadly categorized into lymphoid and myeloid neoplasms depending on the type of white cell proliferation. (medscape.com)
Tissues13
  • However, little is known about EC heterogeneity in hematopoietic tissues. (go.jp)
  • Progenitor cells are often located within specific tissues, close to where they will ultimately function. (thenoveldifference.com)
  • They offer the potential to replace damaged or dysfunctional cells in specific tissues or organs. (thenoveldifference.com)
  • Stem cell-independent pathways during development thus offer a rational strategy for the rapid and simultaneous growth of tissues and stem cell pools. (elsevierpure.com)
  • Progenitor cells are an intermediary step in the development of mature cells in tissues, organs, blood, and the central nervous system. (differencebetween.com)
  • Therefore, progenitor cells are necessary for repairs and to maintain tissues after an injury. (differencebetween.com)
  • They can create new tissues and even whole organs from a few stem cells. (differencebetween.com)
  • Fig. 3: Single-cell transcriptomics of dorsal and ventral MiSTR tissues. (nature.com)
  • Researchers are uncovering ever more details about the complex system of organs, tissues, and different cell types that make up the human immune system. (mdc-berlin.de)
  • HCT/Ps such as amnion/chorion hematopoietic stem/progenitor cells from cord blood and peripheral blood, and reproductive tissues such as semen and oocytes, have the potential for harboring Zika virus. (cdc.gov)
  • Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
Cord blood2
  • Evaluating Interaction of Cord Blood Hematopoietic Stem/Progenitor Cells with Functionally Integrated Three-Dimensional Microenvironments. (wakehealth.edu)
  • The small amount of hematopoietic stem and progenitor cells in cord blood units limits their widespread use in human transplant protocols. (gasyblog.com)
Precursors6
  • Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
  • Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
  • Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)
  • By genetically manipulating EVI1 expression, we were able to alter HSC and progenitor output from precursors in vivo. (elsevierpure.com)
  • Furthermore, unlike for the pro-erythroblast and later stages of erythroid development, no truly reliable and unique positive flow-cytometric markers exist, though it is possible to use negative exclusion markers to deplete a cell population of other precursors and differentiated cells by cell sorting, thus greatly enriching it for the CFU-E activity. (wikipedia.org)
  • When the FIP1L1-PDGFRA fusion gene mutation or point mutations in the PDGFRA gene occur in blood cell precursors, the growth of eosinophils (and occasionally other blood cells, such as neutrophils and mast cells) is poorly controlled, leading to PDGFRA -associated chronic eosinophilic leukemia. (medlineplus.gov)
Hematopoiesis7
  • The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
  • These data demonstrate that allogeneic EPC infusions can augment hematopoiesis and suggest a relationship between BM microvascular recovery and hematopoietic reconstitution in vivo. (duke.edu)
  • Hematopoiesis is the process of blood cell formation. (thenoveldifference.com)
  • The 40-fold increase in childhood megakaryocyte-erythroid and B-cell leukemia in Down syndrome implicates trisomy 21 (T21) in perturbing fetal hematopoiesis. (ox.ac.uk)
  • Here, we propose to uncover the hierarchy and regulatory mechanisms controlling hematopoiesis by combining comprehensive single-cell RNA-Seq analyses, modelling approaches, advanced functional assays, single-cell CRISPR screens, knockout models and epigenetic profiling. (europa.eu)
  • Together, our study is expected to generate a revised and high-resolution hematopoietic model and decipher the regulatory networks that control hematopoiesis. (europa.eu)
  • CD31 + c-kit + cells, but not CD31 - c-kit + cells, isolated from day-9.5 yolk sac give rise to multilineage hematopoiesis in vivo. (elsevierpure.com)
Biology4
  • CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)
  • Emerging single-cell sequencing technologies will undoubtedly enhance our understanding of the complex biology of pancreatic cancer and pave the way for new directions in its clinical diagnosis and treatment. (frontiersin.org)
  • The DKFZ is a multidisciplinary center covering cancer biology from basic molecular and cell biology to clinical research and care. (mdc-berlin.de)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
Fetal3
  • Perturbation of fetal liver hematopoietic stem and progenitor cell development by trisomy 21. (ox.ac.uk)
  • Here, we show that compared with primary disomic controls, primary T21 fetal liver (FL) hematopoietic stem cells (HSC) and megakaryocyte-erythroid progenitors are markedly increased, whereas granulocyte-macrophage progenitors are reduced. (ox.ac.uk)
  • To profile the developmental landscape of fetal HSPCs and their local niche, here, using single- cell RNA -sequencing, we deciphered a dynamic atlas covering 28,777 cells and 9 major cell types (23 clusters) of zebrafish caudal hematopoietic tissue (CHT). (bvsalud.org)
Multipotent progenitors1
  • Fig. 1 Identification of previously unknown compounds promoting human CD34+ cell growth AZD6244 (Selumetinib) Optimization of fed-batch culture period indicated that the highest growth of multipotent progenitors and long-term culture-initiating cells (LTC-ICs) was obtained on day 12 (fig. S3 C AZD6244 (Selumetinib) to E). Similarly the proportion of apoptotic cells was lower at that time when compared with day 16 (fig. S3F). (gasyblog.com)
Functional2
  • In lymphopoiesis, T21 FL lymphoid-primed multipotential progenitors and early lymphoid progenitor numbers are maintained, but there was a 10-fold reduction in committed PreproB-lymphoid progenitors and the functional B-cell potential of HSC and early lymphoid progenitor is severely impaired, in tandem with reduced early lymphoid gene expression. (ox.ac.uk)
  • Progenitor cell therapy may also allow functional reorganization of the auditory pathways including primary auditory cortex (Heschl's gyrus). (intechopen.com)
Differentiate11
  • C) We have identified that islet1 + endothelial progenitors originate from endoderm and finally differentiate into posterior venous ECs in a process dependent on Bmp-Smad signaling, requiring Npas4l-Etsrp function. (go.jp)
  • They differentiate into very specific cells. (thenoveldifference.com)
  • The key difference between hematopoietic stem cells and progenitor cells is that hematopoietic stem cells have the ability to differentiate into different types of cells while progenitor cells are more specific and they differentiate into target cells. (differencebetween.com)
  • Progenitor cells are descendants of stem cells that further differentiate into specialized cell types. (differencebetween.com)
  • Progenitor cells are the cells that originate from stem cells and further differentiate to create specialized cell types. (differencebetween.com)
  • Some cells differentiate into targeted cells, while other cells have the ability to differentiate into more than one type of cell. (differencebetween.com)
  • Hematopoietic stem cells differentiate into multipotent progenitor cells. (differencebetween.com)
  • These multipotent progenitor cells differentiate into either common myeloid progenitor (CMP) or common lymphoid progenitor cells (CLP). (differencebetween.com)
  • On the other hand, progenitor cells are more specific than hematopoietic stem cells and can differentiate into target cells. (differencebetween.com)
  • Cells at the CFU-E stage express some erythropoietin receptor (EpoR), and thus can be induced to terminally differentiate in vitro in 2-3 days in the presence of only erythropoietin (Epo) (together with the basic contents of culture media: FBS, BSA in IMDM). (wikipedia.org)
  • Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
Heterogeneity3
  • Heterogeneity within hematopoietic progenitor populations has considerably limited characterization and molecular understanding of lineage commitment in both health and disease. (europa.eu)
  • Single-cell sequencing is a high-throughput technique that enables detection of genomic, transcriptomic, and epigenomic information at the individual cell level, offering significant advantages in detecting cellular heterogeneity, precise cell classification, and identifying rare subpopulations. (frontiersin.org)
  • Regardless of the therapeutic approach, the heterogeneity of pancreatic cancer cells and the complexity of its tumor microenvironment contribute to the emergence of drug resistance. (frontiersin.org)
Characterization1
  • Recently we have developed an experimental and analytical method, termed massively parallel single-cell RNA-Seq (MARS-Seq), for unbiased classification of individual cells from their native context and successfully applied it for characterization of immune and hematopoietic progenitors. (europa.eu)
Vivo8
  • Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
  • Endothelial progenitors should be devoid of hematopoietic markers and able to form vessels both in vitro and in vivo . (hindawi.com)
  • Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
  • Scholars@Duke publication: Endothelial progenitor cell infusion induces hematopoietic stem cell reconstitution in vivo. (duke.edu)
  • Interestingly, captopril was unable to reduce the proportion of SA2 leukaemic cells in S-phase Captopril on its own at therapeutic doses reduced the proportion of HPP-CFC- 1 in S-phase in vivo regardless of the insult used to induce HPP-CFC-1 into cell cycle. (st-andrews.ac.uk)
  • We further demonstrate that Dlk1 has a negative impact on hematopoietic stem and progenitor cell activity in the aorta-gonad-mesonephros region in vivo, which is recapitulated in co-cultures of hematopoietic stem cells on stromal cells that express varying levels of Dlk1. (ox.ac.uk)
  • Mechanistically, p38α signaling increases expression of inosine-5′-monophosphate dehydrogenase 2 in HSPCs, leading to altered levels of amino acids and purine-related metabolites and changes in cell-cycle progression in vitro and in vivo. (elsevierpure.com)
  • The Cell Manipulations Laboratory's experienced scientists can help you develop and scale up your ex-vivo gene transfer and cell therapy clinical trials or simply transfer your existing at-scale technology to our clean facilities which will enable you to move your research to a safe and effective patient treatment plan. (cincinnatichildrens.org)
Gene15
  • To assess the relative impact of normoxia and hypoxia on the HSC compartment, we performed gene set enrichment analysis (GSEA) of cells within HSC cluster 5 from each culture condition. (confex.com)
  • Patients with Wiskott-Aldrich syndrome (WAS) lacking a human leukocyte antigen-matched donor may benefit from gene therapy through the provision of gene-corrected, autologous hematopoietic stem/progenitor cells. (ucl.ac.uk)
  • Primary outcomes of the long-term study were to establish clinical and biological safety, efficacy and tolerability by evaluating the incidence and type of serious adverse events and clinical status and biological parameters including lentiviral genomic integration sites in different cell subpopulations from 3 years to 15 years after gene therapy. (ucl.ac.uk)
  • An interim analysis shows that the study meets the primary outcome criteria tested given that the gene-corrected cells engrafted stably, and no serious treatment-associated adverse events occurred. (ucl.ac.uk)
  • Our recent expression analysis of the aorta-gonad-mesonephros region showed that the Delta-like homologue 1 (Dlk1) gene is up-regulated in the region of the aorta-gonad-mesonephros where hematopoietic stem cells are preferentially located. (ox.ac.uk)
  • In more than six bicistronic LVs we constructed containing a gene of interest followed by an IRES and the GFP reporter gene, GFP fluorescence was undetectable in transduced cells. (johnshopkins.edu)
  • Thus, such dual-promoter LVs can coexpress multiple transgenes efficiently in a single target cell and will enable many gene transfer applications. (johnshopkins.edu)
  • Here, we performed global microarray-based gene expression measurements on human CD34+ hematopoietic stem and progenitor cells that underwent editing. (biu.ac.il)
  • Researchers and clinicians can contact us for more information about how we can help you with your cell therapy and gene transfer needs. (cincinnatichildrens.org)
  • Combining gene signatures with bioinformatics databases (connectivity mapping) and drug repurposing to target leukaemic whilst sparing normal blood stem cells. (nottingham.ac.uk)
  • Genetic rearrangements and point mutations affecting the PDGFRA gene are somatic mutations, which are mutations acquired during a person's lifetime that are present only in certain cells. (medlineplus.gov)
  • The PDGFRA gene provides instructions for making a receptor protein that is found in the cell membrane of certain cell types. (medlineplus.gov)
  • Wiskott-Aldrich syndrome is caused by mutations in the gene that encodes the Wiskott-Aldrich syndrome protein (WASP), a cytoplasmic protein necessary for normal B- and T-cell signaling. (msdmanuals.com)
  • Gene and cell therapies present novel alternatives to disease management, offering the promise of a single treatment and a lifelong cure. (bvsalud.org)
  • Although challenges remain, investment in the field has started to bear fruit, with a number of gene and cell therapeutics reaching the market in the past decade. (bvsalud.org)
Vitro6
  • Hematopoietic stem and progenitor cells (HPCs) can be maintained in vitro, but the vast majority of their progeny loses stemness during culture. (nih.gov)
  • Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
  • 02:47 and red blood cells in vitro. (yale.edu)
  • Progenitor cell function may be measured post-modification by in vitro CFU assays. (stemcell.com)
  • In addition, captopril alone in vitro reduced the proportion of HPP-CFC-1 in S-phase induced into cell cycle by cytotoxic drugs. (st-andrews.ac.uk)
  • Latest studies demonstrated that aryl hydrocarbon receptor (AhR) antagonists and a notch ligand agonist promote the in vitro enlargement of individual CB cells with repopulating activity long lasting up to 16 weeks in immunodeficient mice (7 8 We created an computerized and continuous moderate delivery program that creates an equivalent enlargement of CB cells with equivalent repopulation properties (9). (gasyblog.com)
Embryonic12
Lymphocytes5
  • In both cell sources, greater than 80% of the cells are lymphocytes, monocytes, or granulocytes. (ebat2018.org)
  • A malignancy in the lymphoid lineage that includes white blood cells such as T lymphocytes and B lymphocytes. (medgadget.com)
  • One of the research areas at the MDC is immunology and inflammation, which covers tumor immunology, DNA repair in B lymphocytes, the role of tumor stroma-immune cell interaction in cancers, the role of innate immune cells in gliomas and neurodegenerative diseases, and the development of immunotherapy strategies. (mdc-berlin.de)
  • CLL is considered the most common form of leukemia in adults and is a type of cancer that starts in white blood cells, known as lymphocytes. (lu.se)
  • The white blood cells (leukocytes) are further divided into phagocytes or myeloid (neutrophils, eosinophils, basophils, monocytes) and immunocytes or lymphoid (lymphocytes). (medscape.com)
Clinical5
  • These cell types are used in cell culture studies to analyze cellular responses in different clinical and physiological settings. (differencebetween.com)
  • No obvious correlations between clinical parameters, including age, karyotype, existence of paroxysmal nocturnal hemoglobinuria clones, pretreatment blood counts, progenitor cell counts, and the response to immunosuppressive therapy (IST), were found. (springermedizin.at)
  • Development of pre-clinical models using stem cell technologies. (nottingham.ac.uk)
  • We will present a summary of the effect of hearing loss on auditory development, existing preclinical and clinical data on progenitor cell therapy, and its potential role in the (re)habilitation of non-genetic SNHL. (intechopen.com)
  • Examples are CRISPR/Cas9-mediated mutagenesis, genetic mouse models, cell-fate-mapping, organoids and tumor explants, as well as GMP production of pharmaceuticals and many years of experience in conducting clinical studies. (mdc-berlin.de)
Caudal hematopoietic2
  • When these islet1 -lineage ECs were specifically ablated, most of the HSPCs were lost from the caudal hematopoietic tissue (CHT), a transient vascular niche for HSPCs in zebrafish (Figure 1B). (go.jp)
  • islet1 + -derived ECs (green) are mainly located in the caudal hematopoietic tissue (CHT), a transient vascular niche for HSPCs. (go.jp)
Pluripotent stem2
  • The approach, named microfluidic-controlled stem cell regionalization (MiSTR), exposes pluripotent stem cells to signaling gradients that mimic developmental patterning. (nature.com)
  • Using our recently established haploid human pluripotent stem cells (hPSCs), we generated a genome-wide loss-of-function library targeting 18,166 protein-coding genes to define the essential genes in hPSCs. (nature.com)
Autologous1
  • Cell Replacement Therapy for Parkinson's Disease - Evaluating the potential of autologous grafting. (lu.se)
20222
  • Cell Reports Cell Rep Dec 13;41(11):111825, 2022. (stjude.org)
  • Nat Cell Biol Oct;24(10):1475-1486, 2022. (stjude.org)
Precursor1
Humans2
  • In the central nervous system of humans, there are three types of fully differentiated cells known as neurons, astrocytes, and oligodendrocytes. (differencebetween.com)
  • The Nilsson lab focuses on how inborn genetic variation influences blood cell formation and blood cancer risk in humans. (lu.se)
Endothelial cells6
  • Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
  • Neither AT alone in control media nor conditioned media nor AT-stimulated cells affected numbers of endothelial cells in the plug or plug's vascularization. (hindawi.com)
  • The process of neovascularization can be influenced not only by the residual endothelial cells but also by the circulating cells. (hindawi.com)
  • Researchers identify the origin of endothelial cells that constitute the vascular niche for hematopoietic stem and progenitor cells in zebrafish. (go.jp)
  • Endothelial cells (ECs) line blood vessels and can serve as specialized vascular niches for hematopoietic stem and progenitor cells (HSPCs), a special environment where HSPCs reside and self-renew. (go.jp)
  • Platelet endothelial cell adhesion molecule-1 (PECAM-1) (CD31) is an adhesion molecule expressed on endothelial cells and subsets of leukocytes. (elsevierpure.com)
Genetic2
  • Approximately half of congenital SNHL is hereditary and is the result of genetic mutations causing improper development of cochlear hair cells. (intechopen.com)
  • Olariu V, Manesso E, commitment and Peterson C. 2017 A deterministic method for estimating free energy genetic network reprogramming paths landscapes with applications to cell commitment and reprogramming paths. (lu.se)
Immune4
  • These remarkable cells play a crucial role in the production and maintenance of the body's blood and immune cell supply. (thenoveldifference.com)
  • The many distinct sorts of mature blood cells, like red blood cells for carrying oxygen, white blood cells for immune protection and platelets for wound clotting. (medgadget.com)
  • How is the tissue specificity of immune cells generated and how is it affected by environmental influences, plasticity and the tissue microenvironment? (mdc-berlin.de)
  • For example, you have a cancer cell, and you want antibodies that can kill the cancer cell or activate a certain immune cell or so on, and then you develop the antibodies based on this. (lu.se)
Flow Cytometry1
  • Results HPDSCs Are Rich in Both Hematopoietic and Nonhematopoietic Stem Cells The overall cell types as determined by flow cytometry analysis are comparable between HPDSCs and UCB. (ebat2018.org)
Early lymphoid1
Mice3
  • BALB/c mice were treated with total body irradiation (TBI) and then infused with C57Bl6-derived endothelial progenitor cells (EPCs) to augment endogenous BM EC activity. (duke.edu)
  • To analyze its function, we studied Dlk1 expression in wild-type and hematopoietic stem cell-deficient embryos and determined hematopoietic stem and progenitor cell activity in Dlk1 knockout and overexpressing mice. (ox.ac.uk)
  • No significant alteration of hematopoietic homeostasis and HSC function is observed in young Pus10-/- mice, while aged Pus10-/- mice exhibit mild alteration of hematopoietic homeostasis and HSC function. (haematologica.org)
Blood25
Regenerative Medicine1
Genome2
  • 2018) describe the global transcriptional response to individual components of Cas9/AAV6-mediated genome editing in hematopoietic stem and progenitor cells. (biu.ac.il)
  • studies on cell cycle regulation and genome instability. (ncl.ac.uk)
Subpopulation2
  • Most clusters could be computationally assigned to a defined hematopoietic subpopulation, including progenitor cells (clusters 0 to 4) and a single transcriptionally defined HSC population (cluster 5). (confex.com)
  • Specifically, a subpopulation of cells with a phenotype of CD34+/CD45? (ebat2018.org)
Allogeneic1
  • Globally, it is estimated that 120 000 corneal transplantations and 18 000 transplantations of allogeneic haematopoietic progenitor cells took place in the year 2000. (who.int)
Megakaryocyte1
  • Commensurately, HSC and megakaryocyte-erythroid progenitors show higher clonogenicity, with increased megakaryocyte, megakaryocyte-erythroid, and replatable blast colonies. (ox.ac.uk)
Self-renewal1
Acute myeloid1
  • Inhibition of HOX/PBX dimer formation leads to necroptosis in acute myeloid leukemia cells ONCOTARGET. (nottingham.ac.uk)
Functionally1
  • 03:06 cell functionally in FINA? (yale.edu)
Clusters1
  • We identified 6 distinct clusters (clusters 0 to 5) in dimension-reduction (UMAP) analysis, with a comparable distribution of cells per cluster between normoxic and hypoxic cultures. (confex.com)
Populations2
  • Cell division tracking further showed that UM171 does AZD6244 (Selumetinib) not impact the division rate of phenotypically primitive populations (fig. S7). (gasyblog.com)
  • It unlocks novel dimensions by uncovering elusive cell populations and tumor heterogeneities for early detection, unraveling mechanisms of treatment resistance, tailoring therapies to individuals, and illuminating metastasis and cachexia pathways, thereby opening fresh avenues for preventive strategies. (frontiersin.org)