Progenitor cell free system. Medical search. Frequent questions

However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
We first optimized a serum-free, miniature erythroid differentiation system starting from primary human CD34 cells, the exact type of cells we would ultimately like to target in vivo ( Figure 1 ). (haematologica.org)
Characterization and validation of the small scale erythroid differentiation system used for small molecule screen. (haematologica.org)
Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
MYB is an essential regulator of primitive human hematopoiesis in human pluripotent stem cell differentiation cultures. (nih.gov)
MYB is well recognized to be a key regulator of definitive hematopoiesis that plays an important role in the maintenance and multilineage differentiation of hematopoietic stem cells (HSCs). (nih.gov)
To explore the role of MYB in human hematopoietic development we have inactivated the gene by bi-allelic TALEN-supported gene targeting in several lines of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), and subjected these cells to hematopoietic differentiation in well-defined cell culture conditions. (nih.gov)
Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. (stemcell.com)
Previous studies have indicated that newly formed oligodendrocytes are dynamic cells whose production, survival, and differentiation depend upon axonal influences. (rupress.org)
Intestinal Neurogenin 3 directs differentiation of a bipotential secretory progenitor to endocrine cell rather than goblet cell fate. (revacure.nl)
While pluripotent embryonic stem cells have an almost unlimited differentiation potential to become any type of cell, multipotent adult stem cells have a much more limited ability to differentiate. (iastate.edu)
Aged hematopoietic stem cells (HSC) exhibit compromised reconstitution capacity and differentiation-bias towards myeloid lineage, however, the molecular mechanism behind it remains not fully understood. (haematologica.org)
This technique allowed the team to detect cell inefficiencies and develop a new understanding of the underlying cell biology during the differentiation of PSCs. (nextbigfuture.com)
We present a predictive bioprocess design strategy employing cell- and molecular-level analysis of rate-limiting steps in human pluripotent stem cell (hPSC) expansion and differentiation, and apply it to produce definitive endoderm (DE) progenitors using a scalable directed-differentiation technology. (nextbigfuture.com)
We have recently shown that myeloid leukaemias in mouse and human are often driven by rare leukaemia (cancer) stem cells which are at the progenitor stage of differentiation, but have activated the self-renewing cell division pathway normally used only by HSCs. (edu.hk)

Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). (sigmaaldrich.com)
In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)

Mesenchymal stem cells (MSCs) are multipotent stem/progenitor cells which can differentiate to several mesodermal derivatives and possess an ability to secrete factors involved in neovascularization and immunomodulation [ 1 , 2 ]. (hindawi.com)
With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)
At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
Introduction Mesenchymal stromal cells (MSCs) are adult, fibroblast-like multipotent cells characterized by the ability to differentiate into tissues of mesodermal origin, such as adipocytes, chondroblasts, and osteoblasts (Friedenstein et al. (pdffox.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
Stem cells rely on extracellular signals produced by the niche, which dictate their ability to self-renew, expand and differentiate. (karger.com)
This was accomplished by implanting rat adult hippocampal progenitor/stem cells (AHPCs) - which normally differentiate into neurons, astrocytes, and oligodendrocytes - into zebrafish embryos during the blastula stage, an environment with almost unlimited potential for stem cells. (iastate.edu)
Proteins are the machinery of the cell, responsible for executing the functions essential for cells to operate, survive, differentiate and divide. (lu.se)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)

Estrogens are female sex hormones that have genotoxic and proliferation-enhancing effects in cells. (helsinki.fi)
For several decades, neovascularization is believed to be accomplished by proliferation of mature endothelial cells residing in the local vessels through the process of angiogenesis [ 6 ]. (hindawi.com)
However, inactivation of MYB severely abrogated proliferation of the primitive erythroid and mixed erythroid-macrophage-megakaryocyte progenitors. (nih.gov)
The present work introduces a computational implementation of some principles of a theory of organism development, namely that the default state of cells is proliferation and motility, and includes the principle of variation and organization by closure of constraints. (nature.com)
In the present model, the bioelectric context of cells and tissue is the field responsible for organization, as it regulates cell proliferation and the level of communication driving the system's evolution. (nature.com)
Starting from a depolarized (proliferative) cell, the organism grows to a certain size, limited by the increasingly polarized state after successive proliferation events. (nature.com)
A) The biological activity of Human Recombinant G-CSF, ACF was tested by its ability to promote the proliferation of mouse NFS-60 cells. (stemcell.com)
Cell proliferation was measured using a fluorometric assay method. (stemcell.com)
The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. (stemcell.com)
Altogether our FACS (fluorescence-activated cell sorter) analyses reveal that the neonatal subventricular zone is far more heterogeneous than previously suspected and our studies provide new insights into the signals and mechanisms that regulate their self-renewal and proliferation. (karger.com)
The most well characterized regulatory steps of adult hippocampal neurogenesis are the rate of progenitor cell proliferation and the survival rate of new DGCs. (jneurosci.org)
We found that NSPCs treated with VEGF-A showed lower rates of cell death and increased cell proliferation,' says senior author Tetsuya Taga. (news-medical.net)
Eph/ephrin signaling is a cell-to-cell communication pathway, which regulates cell migration and proliferation. (5dok.org)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)

Transposon-mediated rescue of MYB expression in MYB-null cells efficiently restored both the primitive hematopoietic progenitors and immature myeloid cells. (nih.gov)

Human CD34 + hematopoietic stem and progenitor cells purified from umbilical cord or adult peripheral blood were cultured in a two-phase liquid culture system in a serum-free medium for 21 days. (haematologica.org)
The modified lines including MYB+/+, MYB+/- and MYB-/- were differentiated into hematopoietic cells in our new established serum-free, cytokine-free culture system with minimal BMP4 exposure for mesoderm induction. (nih.gov)
Using fetal bovine serum (FBS) as a source of the erythropoietic activity and serum-free cultures of fetal mouse livers (FMLC assay) as a detection system, we found that FBS stimulated colony formation from late erythroid progenitor cells (CFU-E) in a dose-dependent fashion. (uni-luebeck.de)
Embryoid bodies were then plated on tissue-culture plastic dishes and cultured in serum-free, defined media (ITSFn). (nih.gov)

Finally, neurons developing within a highly active environment exhibited a delayed maturation when their intrinsic electrical activity was reduced by the cell-autonomous overexpression of Kir2.1, an inward-rectifying potassium channel. (jneurosci.org)
These findings support that Nurr1 exerts a protective effect on adult mDA neurons in a cell-autonomous manner Indeed, several intrinsic mechanisms associated with Nurr1-mediated cell survival have been identified. (justia.com)
Cell 137: 47-59) has identified an unexpected effect of Nurr1 which is expressed in glial cells in response to stimuli which trigger inflammation, and this Nurr1 suppresses the production of pro-inflammatory cytokines that cause the death of mDA neurons, but does not suggest a therapeutic effect through induction of Nurr1 expression. (justia.com)
In particular, glial cells in nerve tissues include astrocytes and microglial cells and are accessory cells that aid in promoting the functions and survival of neurons. (justia.com)
However, under a pathologic condition, the glial cells are instead activated to set up an environment in which the glial cells cause damage to neurons (M1 activation). (justia.com)
Meanwhile, it is known that glial cells with an M1 phenotype which set up a diseased environment can be converted into glial cells with an M2 phenotype which promote a therapeutic environment in which the survival and regeneration of neurons is set up. (justia.com)
Immunolabeling procedures to identify differentiated cell-types revealed the highest percentage of transplanted AHPCs were TuJ1-labeled (73.4%), which distinguishes immature neurons. (iastate.edu)
A team of scientists, led by Stuart Lipton, M.D., Ph.D., professor and director of the Neuroscience and Aging Research Center at Sanford-Burnham Medical Research Institute (Sanford-Burnham), recently discovered why cerebellar granule cell neurons in patients suffering from ataxia-telangiectasia (A-T) were unable to repair DNA damage and thus died. (sbpdiscovery.org)
Knowing that ATM-mediated activation of MEF2D promotes survival in cerebellar neurons in response to DNA damage may provide a therapeutic opportunity for A-T. For example, if we can confirm that defects in the ATM-MEF2D signal contribute to A-T, we can search for molecules that enhance MEF2D activity to 'revive' the DNA repair system. (sbpdiscovery.org)
In addition to the olfactory neurons, the epithelium is composed of supporting cells, Bowman glands and ducts unique to the olfactory epithelium, and basal cells that allow for the regeneration of the epithelium, including the olfactory sensory neurons. (medscape.com)
The idea to use transplants of dopa- ment of protocols that allow generation of fully functional mine-producing cells to substitute for the lost midbrain and safe midbrain dopamine neurons from stem cells. (lu.se)
VM), showed that the recovery of motor functions induced implanted either (1) as a solid piece in the lateral ven- by the grafted fetal dopamine neurons was well cor- tricle6 or a cortical cavity8 adjacent to the denervated related with the extent of graft-derived reinnervation caudate-putamen, or (2) as a crude cell suspension of the host caudate-putamen. (lu.se)

Dr. Nguyen (pronounced as "Gwen") earned a bachelor degree in Biochemistry and Cell Biology at the University of California, San Diego, and a PhD in Integrated Biology and Disease at the University of Southern California, Los Angeles. (selectbiosciences.com)
Recent developments in cancer biology have identified the existence of a sub-poplulation of cells - cancer stem cells (CSC) that are resistant to most traditional therapies (e.g. chemotherapy and radiotherapy) and have the ability to repair their damaged DNA. (docksci.com)
My lab also studies the role of TERT in lung stem cell biology and repair of acute lung injury, which is a culprit gene mutation in IPF. (stanford.edu)
In 2002 he became Director of the Stanford Cancer/Stem Cell Institute, which was split into the Stanford Institute of Stem Cell Biology and Regenerative Medicine, and the Stanford Cancer Center in 2003. (edu.hk)
His research also encompasses the phylogeny and developmental biology of the cells that make up the blood-forming and immune systems. (edu.hk)
Increasing evidence emphasize that a substantial variance of protein expression is conveyed at the levels of protein synthesis and degradation, and that post-transcriptional control of gene expression plays a critical role in multiple aspects of cell biology, including coordination of cellular processes and rapid alteration of cellular phenotype. (lu.se)
Frontiers in cell and developmental biology. (lu.se)

A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
The cells were harvested at different time points for cell-sorting based on the expression of MYB-YFP and CD34 cell surface marker on day 6 and day 12. (nih.gov)

After her graduate studies, she joined Prof Karl Tryggvason's laboratory at Duke-NUS Medical School as a research fellow in 2013 where her main focus has been to culture endothelial cells for therapeutic purposes and developing different cell models for in vitro drug testing. (selectbiosciences.com)
Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. (stemcell.com)

Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)
In vitro models of patient desmoid tumor cells revealed a direct effect of Tazemetostat on Wnt pathway activity. (xenbase.org)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
Using biologically relevant elastic substrates to study cell function in vitro has proven beneficial, as the in vivo microenvironment usually is much softer than rigid plastic dishes. (5dok.org)
Although other irritants in the smoke may have contributed to the incident, there is supporting evidence that stable strontium can stimulate the release of histamine from mast cells in vitro (ATSDR 2001e). (cdc.gov)

Studies have shown that cell-to-cell mitochondrial transfer plays an essential role in regulating cardiovascular system development and maintaining normal tissue homeostasis under physiological conditions. (frontiersin.org)
Mitochondria not only serve as power plants in cells but also act as crucial regulators in many biological processes, including reactive oxygen species (ROS) signaling, redox balance, calcium homeostasis, protein quality control, and programmed cell death ( 3 , 4 ). (frontiersin.org)
Studies have shown that cells can eliminate defective mitochondria by delivering them to recipient cells (such as macrophages) to maintain homeostasis. (frontiersin.org)
The system reaches homeostasis, with a depolarized core (proliferative cells) surrounded by a rim of polarized cells (non-proliferative in this condition). (nature.com)
This theory would deal with the way an organism spontaneously grows from a single cell to a complete and complex organism, introducing organization at the different development stages and homeostasis for a mature organism. (nature.com)

Scientists in Canada have overcome a key research hurdle to developing regenerative treatments for diabetes and liver disease with a technique to produce medically useful amounts of endoderm cells from human pluripotent stem cells. (nextbigfuture.com)

Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)

Our data show that selective silencing of α-globin expression in erythroid cells is pharmacologically feasible, and IOX1 is a lead compound to developing new therapy to treat β-thalassemia through the novel pathway of downregulating α-globin expression. (haematologica.org)

One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
Adult neurogenesis is a complex process whereby neural progenitor cells adopt a neuronal fate, migrate, and mature over several weeks. (jneurosci.org)
In order to better understand how adult stem cells choose their fate, two main factors were investigated: intrinsic versus extrinsic influences. (iastate.edu)
Through this knowledge, adult stem cells and their cell regulation behaviors can be further investigated to potentially find similar drug options that may mimic the regulation of their expression. (iastate.edu)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)

In this review, we summarized the mechanism of mitochondrial transfer in the cardiovascular system and outlined the fate and functional role of donor mitochondria. (frontiersin.org)
This preliminary analysis reveals that these brain stem cells are likely influenced by intrinsic cell machinery - regulation of gene expression - than their environment when choosing their fate. (iastate.edu)
targeted cell Loss) and, with quantitative cell division tracking and fate monitoring, identify and overcome key suspension bioprocess bottlenecks. (nextbigfuture.com)
This signaling pathway is a highly conserved cell-to-cell communication system that regulates cell fate in development and disease. (5dok.org)
Importantly, the proteomic make-up of blood stem cells and progenitors can regulate their cell fate and susceptibility to initiation as well as progression of leukemia. (lu.se)

Here we define a 4-color flow cytometry panel using CD133, LeX, CD140a, NG2 to define a neural stem cell (NSC) as well as 4 classes of multipotential progenitors and 3 classes of bipotential progenitors, several of which have not been described previously. (karger.com)

Adapting process operating conditions to pivotal parameters (single cell survival and growth rate) in a cell-line-specific manner enabled adherent-equivalent expansion of hPSCs in feeder- and matrix-free defined-medium suspension culture. (nextbigfuture.com)
After 5-7 days selection in ITSFn, adherent cells were trypsinized, triturated to a single cell suspension, and re-plated on tissue culture dishes coated with 15 ug/ml poly-L-ornithine (Sigma) and 1 ug/ml human plasma fibronectin (Gibco). (nih.gov)

Allogeneic hematopoietic stem cell transplantation is curative but it requires a histocompatible donor and is associated with significant morbidity and mortality, so it is reserved for severe cases of PNH with aplastic anemia or transformation to leukemia. (medscape.com)
Globally, it is estimated that 120 000 corneal transplantations and 18 000 transplantations of allogeneic haematopoietic progenitor cells took place in the year 2000. (who.int)

The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)
That led him to years of research and development in the regenerative medicine field and his venture as president and CEO of Fort Myers-based EmCyte, a medical manufacturing company that produces devices used for extracting platelet-rich plasma (PRP) and stem cell biologics. (emcyte.com)
Scalable Amounts of Liver and Pancreas Precursor" New Stem Cell Method Developed Increasing the available stem cells by 35 times or more will open the path to more regenerative medicine treatments using stem cells. (nextbigfuture.com)

It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). (stemcell.com)

In response to this challenge, the International Society for Cellular Therapy formulated minimal criteria for defining MSCs in order to create a broader consensus for more uniform characterization of these cells (Dominici et al. (pdffox.com)
Although extensive bat surveys have been conducted, no infectious bat CoVs have been isolated from cell cultures, which hinders characterization of bat CoVs and evaluation of the risks posed by these viruses to public health. (cdc.gov)

With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
In a new study published in Inflammation and Regeneration , researchers from Tokyo Medical and Dental University (TMDU) investigated the effects of hypoxic (low oxygen) conditions on the neural stem cell niche during development. (news-medical.net)
Self renewal is the property that distinguishes stem cells and progenitors, and in the blood-forming system explains why haematopoietic stem cells (HSCs), not progenitors, are the only cells capable of providing rapid and sustained regeneration of the blood-forming system after ablation by cancer chemo- and radiotherapies. (edu.hk)
Victoria Ptasinski defended her Ph.D. thesis 'Alveolar Progenitor Cells in Lung Damage and Regeneration in Pulmonary Fibrosis' on Monday, November 27, 2023. (lu.se)
There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)

Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)
We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
Cells sense and respond to the mechanics of their substrate - be it the extracellular matrix, neighboring cells or artificial matrix in cell culture. (5dok.org)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)

It is essential to have sensitive and reproducible methods of either quantifying or isolating these stem cells and progenitors to understand their intrinsic properties and how extrinsic signals regulate their development. (karger.com)
His lab also studies the molecular signals that regulate lung stem cell activity, and how the signals can be manipulated to restore their activity. (stanford.edu)
We focus our attention on lung stem cells and the molecular signals that regulate their activity to repair and regenerate lung tissue after injury. (stanford.edu)

Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. (hindawi.com)
We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. (hindawi.com)
Previous studies demonstrated that MSCs could ameliorate the pathology associated with ischemic heart disease, ischemic stroke, and peripheral vascular disease by releasing several critical factors that increase cell survival, provide an appropriate microenvironment for repairing damaged tissue, and induce neovascularization [ 3 - 5 ]. (hindawi.com)
However, recent studies showed that MSCs derived from distinct sources might produce distinct combination of factors that exert different effects on target cells [ 15 , 16 ]. (hindawi.com)
In addition to their stem/progenitor properties, mesenchymal stromal cells (MSCs) possess broad immunoregulatory properties that are being investigated for potential clinical application in treating immune-based disorders. (pdffox.com)
An informed view of the scope of this clinical potential will require a clear understanding of the dynamic interplay between MSCs and the innate and adaptive immune systems. (pdffox.com)
2007). In addition to their stem/progenitor properties, MSCs have also been shown to possess broad immunoregulatory abilities and are capable of influencing both adaptive and innate immune responses. (pdffox.com)
This ability of MSCs to adopt a different phenotype in response to sensing an inflammatory environment is not captured in assays that are commonly used to characterize these cells, but it is crucial for understanding their therapeutic potential in immune-mediated disorders. (pdffox.com)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Max Perutz was able to unravel the structure of hemoglobin, the red blood cell protein that carries oxygen. (revacure.nl)
In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
The researchers used genetically engineered mice to show that myoctye-enhancer factor 2D (MEF2D), a transcription factor that turns on specific genes involved in cell survival, is activated after binding to a protein known as A-T mutated (ATM). (sbpdiscovery.org)
Most type II PNH cells (total lack of GPI-linked protein) are due to a frame shift mutation occurring in the early hematopoietic progenitor cells, resulting in same mutation in all blood cell lines. (medscape.com)
The RET gene provides instructions for producing a protein that is involved in signaling within cells. (medlineplus.gov)
This protein appears to be essential for the normal development of several kinds of nerve cells, including nerves in the intestine. (medlineplus.gov)
Mutations in the RET gene that cause Hirschsprung disease result in a nonfunctional version of the RET protein that cannot transmit signals within cells. (medlineplus.gov)
The EDNRB gene provides instructions for making a protein called endothelin receptor type B. When this protein interacts with other proteins called endothelins, it transmits information from outside the cell to inside the cell, signaling for many important cellular processes. (medlineplus.gov)
DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)

Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)

We found that MYB was not required for initial hematopoietic commitment of nascent mesoderm and emergence of primitive, yolk sac-type human hematopoietic progenitors. (nih.gov)

Xeno-free media that do not rely upon high levels of bFGF have been developed that contain only humanised or synthetic components and are engineered to support the undifferentiated growth and expansion of human ES cells. (ddw-online.com)
Use of human feeders derived under xeno-free conditions can maintain the xeno-free nature of the culture system and allow for the use of low levels of bFGF. (ddw-online.com)

For HIV patients in particular, these would be T cells and blood-forming stem cells called hematopoietic stem and progenitor cells (HSPCs), which give rise to the body's blood and our entire blood-based immune system. (iflscience.com)
You can take blood-forming stem cells out of a patient, edit them, and transplant them back," Rossi explains in a news release . (iflscience.com)

It also makes it possible for a person's own immune cells to attack HIV -- without being susceptible to it. (iflscience.com)

1 The accumulation of free excess α-globin chains in red blood cells and their precursors, as a result of the decreased production of β-globin, is believed to be the main pathophysiological mechanism leading to hemolytic anemia and ineffective erythropoiesis in β-thalassemia. (haematologica.org)

In addition, MYB-negative hESC/iPSC lines demonstrated major defects in myeloid cell development and completely failed to generate mature granulocytes. (nih.gov)
The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. (stemcell.com)

Due to its location on the X chromosome, and due to X inactivation in female somatic cells, only one mutation is required in either males or females to abolish the expression of GPI-linked proteins. (medscape.com)

The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)
1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
Therefore, the present inventors have found that various neurologic disorders based on inflammation may be treated since transcription factors Nurr1 and Foxa2 interact with each other to convert glial cells from an M1 phenotype to an M2 phenotype which sets up a therapeutic environment, when the transcription factors are overexpressed in the glial cells. (justia.com)
One of the possible reasons for the failure of the present therapeutic regimens is the existence of a relatively rare cell population known as cancer stem cells (CSCs) that are resistant to or not targeted by present therapeutic modalities. (docksci.com)
Our data, thus, highlight the novel therapeutic potential of PCG-EPCs as a combined 'growth and delivery system' to achieve an accelerated fibrosis-free healing of dermal lesions, including diabetic wounds. (sciencecentral.in)
In recent years his work has included studying the potential of CD47 as a cancer therapeutic and identifying cancer stem cells from a variety of blood and solid cancers. (edu.hk)
The new reverse genetics system described here will facilitate future therapeutic studies for these two life-threatening arenaviruses. (cdc.gov)

Prof Weissman is an expert in the fields of hematopoiesis, leukemia, and hematopoietic stem cells (HSC), and most recently, the clonal events leading from HSC to leukemia stem cells. (edu.hk)
Future work will include detailed proteomic profiling of ontogeny-specific leukemic features, investigation of the mechanistic details of hematopoietic stem cell plasticity, as well as specific types of post-translational modifications of particular importance in early hematopoiesis. (lu.se)

They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. (stemcell.com)
1 This includes human cells for transplantation such as haematopoietic stem cells from bone marrow, peripheral blood or cord blood. (who.int)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

Cancer-free prospectively purified HSCs regenerate the haematopoietic system of patients as rapidly as a marrow or mobilized blood transplant, but without the risk of re-seeding the body with cancer cells. (edu.hk)

While significant progress has been made towards more defined culture conditions, development of animal-free culture systems has lagged. (ddw-online.com)
James Miller introduced the living systems theory 4 , where each system must include a number of critical subsystems, dealing with their structure, interaction, behavior and development, that can be applied from simple cells to full organisms. (nature.com)
While other factors may also contribute to NSPC maintenance, these results shed new light on the composition of the neural stem cell niche during development, and may serve as a foundation for further studies of self-organization of the hypoxic niche. (news-medical.net)
2023) Organization of self-advantageous niche by neural stem/progenitor cells during development via autocrine VEGF-A under hypoxia. (news-medical.net)
We propose an opportunity to target the cancer cell/microenvironment interface instead of the Notch pathway itself in the development of cancer therapies. (5dok.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)

Now, researchers in Japan have shed new insight into the dynamics of the neural stem cell niche, the home of stem cells in the brain. (news-medical.net)
The TMDU-led research team set out to investigate the effects of low oxygen conditions within the neural stem cell niche using a cell culture model of NSPCs isolated from the forebrains of embryonic mice. (news-medical.net)
We used conjugated polymers to develop a novel neural stem cell culture substrate with anchored growth factors to promote cell self-renewal. (5dok.org)

MatTek's NHCE cells are characterized by their cobblestone morphology and positive expression of cytokeratin 3/12, cytokeratin 15 and aldehyde dehydrogenase 3A1. (mattek.co.kr)

In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)

Carcinogenesis is introduced through a localized event (a spot of depolarized cells) or by random depolarization of cells in the tissue, which returns cells to their initial proliferative state. (nature.com)
This simplified model of embryogenesis, tissue organization and carcinogenesis, based on non-excitable cells' bioelectric properties, can be made more realistic with the introduction of other components, like biochemical fields and mechanical interactions, which are fundamental for a more faithful representation of reality. (nature.com)
Our experimental approaches involve mouse genetics, single cell genomics, organoid culture, lung slice culture, and we perform histological analysis of lung tissue using advanced fluorescence microscopy technologies. (stanford.edu)

Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
Although this approach eliminates the need for feeder layers, the system still contains undefined animal proteins, as well as possible contaminants. (ddw-online.com)
Clinical manifestations of PNH occur when a HSC clone carrying somatic PIGA mutations acquires a growth advantage and differentiates, generating mature blood cells that are deficient of GPI-anchored proteins. (medscape.com)
This classification was abandoned because of the observation that surface proteins were missing not only in the RBC membrane but also in all blood cells, including the platelet and white cells. (medscape.com)
The common denominator in the disease, a biochemical defect, appears to be a genetic mutation leading to the inability to synthesize the glycosyl-phosphatidylinositol (GPI) anchor that binds these proteins to cell membranes. (medscape.com)
The absence of these regulating proteins results in uncontrolled amplification of the complement system. (medscape.com)

We have therefore investigated the role of matrix stiffness in Notch signaling in breast cancer cells. (5dok.org)

Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
We attempted to isolate bat CoVs and virus RNA from cell cultures and from Leschenault rousette bats ( Rousettus leschenaulti ) orally infected with intestinal tissues and contents from a lesser dog-faced fruit bat ( Cynopterus brachyotis ). (cdc.gov)
All tissues originate from stem cells. (bvsalud.org)

Diabetes mellitus (DM)-induced endothelial progenitor cell (EPC) dysfunction causes impaired wound healing, which can be rescued by delivery of large numbers of 'normal' EPCs onto such wounds. (sciencecentral.in)

Anatomy19

Organisms3

Diseases1

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment6

Phenomena and Processes15

Disciplines and Occupations2

Information Science2

Differentiation17

Embryonic stem6

Multipotent5

Differentiate5

Proliferation15

Myeloid cells1

Serum-free4

Neurons12

Biology7

CD344

Endothelial Cells3

Vitro5

Homeostasis5

Pluripotent stem1

Fetal1

Erythroid1

Adult6

Fate5

Bipotential1

Adherent2

Allogeneic2

Regenerative medicine3

Colony formation1

Characterization2

REGENERATION5

Extracellular4

Regulate3

MSCs9

Protein9

Mesenchymal1

Mesoderm1

Xeno-free2

Blood-forming st2

Immune cells1

Precursors1

Granulocytes2

Inactivation1

Therapeutic7

Hematopoiesis2

Bone marrow4

Haematopoietic1

Development6

Neural stem3

Morphology1

Functional1

Tissue3

Proteins6

Cancer Cells1

Tissues3

Dysfunction1