• Ghasemi-Mobarakeh L, Prabhakaran MP, Tian L, Shamirzaei-Jeshvaghani E, Dehghani L, Ramakrishna S. Structural properties of scaffolds: Crucial parameters towards stem cells differentiation. (wjgnet.com)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • Self-renewal and differentiation are tightly controlled to maintain haematopoietic stem cell (HSC) homeostasis in the adult bone marrow 1,2 . (elsevierpure.com)
  • During fetal development, expansion of HSCs (self-renewal) and production of differentiated haematopoietic cells (differentiation) are both required to sustain the haematopoietic system for body growth 3,4 . (elsevierpure.com)
  • Experiments revealed that the fusion protein activates unusual signaling pathways during PSC differentiation leading to changes in cycling and survival of the red cells. (ca.gov)
  • Unlimited self renewal capacity and differentiation potential make human pluripotent stem cells (PSC) a promising source for the ex vivo manufacture of red blood cells (RBC) for safe transfusion. (ca.gov)
  • We present here evidence that ic-MPL dimerization induces erythropoietin (EPO)-independent erythroid differentiation from hESC by inducing the generation of erythroid progenitors and by promoting more efficient erythroid maturation with increased RBC enucleation as well as increased gamma:epsilon globin ratio and production of beta-globin protein. (ca.gov)
  • Here, I examined in lumpfish eyes, the expression of zonula occuldens-1 (ZO-1), chloride intracellular channel protein 2 (CLIC2), proliferating cell nuclear antigen (PCNA), cluster of differentiation 45 (CD45) and cluster of differentiation 10 (CD10), known markers of vascular and hematopoietic tissues in mammals. (mun.ca)
  • We inferred their differentiation trajectory and identified three highly proliferative oligopotent progenitor populations downstream of hematopoietic stem cells (HSCs)/multipotent progenitors (MPPs). (unibg.it)
  • UCB's advantages over other sources of stem cells such as adult bone marrow and embryonic stem cells include easy procurement, minimal risk to the donor, excellent proliferation and differentiation, immediate availability, and autologous use. (sajbl.org.za)
  • During the differentiation process from hematopoietic progenitor cells, chromosomes in the cell nucleus aggregate and the cell becomes a mature erythrocyte by subsequently losing this nucleus (enucleation). (sciencedaily.com)
  • According to rough calculations, the amount of culture medium needed to produce enough erythrocytes for a normal transfusion pack (containing one trillion units) is 1,000 -- 2,000 liters when using direct differentiation from undifferentiated iPS cells, whereas 50-100 liters is sufficient with the newly developed method. (sciencedaily.com)
  • Several investigations [ 5 - 7 ] have been carried out with isolated embryonic, fetal, and adult SCs in a well-defined culture microenvironment to define the sequential steps and intracellular pathways that are involved in their differentiation into the specific cell lineages. (biomedcentral.com)
  • The high proliferation and differentiation capacity of fetal MSCs and the role of fetal pancreatic-derived MSCs (FPMSCs) in islet generation make them good candidates for diabetes treatment. (biomedcentral.com)
  • A side-by-side comparison was made to evaluate the effect of each serum on proliferation rate, cell cycle, senescence, multi-lineage differentiation capacity, immunophenotype, and tumorigenesis of FPMSCs. (biomedcentral.com)
  • Flow cytometry analysis and three-lineage differentiation ability demonstrated that fibroblast-like cells obtained from primary culture had MSCs' characteristics. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) have received more attention due to their proliferation and differentiation capacity and low immunogenicity. (biomedcentral.com)
  • As development progresses, nephron progenitors switch from glycolysis to mitochondrial respiration for energy-mediated by an unknown mechanism-and undergo differentiation. (lww.com)
  • In addition to identifying VHL as a critical regulator of nephron progenitors' metabolic switching, the authors' findings demonstrate that this switch also plays a large role in the differentiation process, and suggest that VHL is required for normal kidney development. (lww.com)
  • A switch from glycolysis to mitochondrial respiration drives these cells toward differentiation, but the mechanisms that control this switch are poorly defined. (lww.com)
  • To explore VHL as a regulator defining nephron progenitor self-renewal versus differentiation, we bred Six2-TGC tg mice with VHL lox/lox mice to generate mice with a conditional deletion of VHL from Six2 + nephron progenitors. (lww.com)
  • Our findings identify a novel role for VHL in mediating nephron progenitor differentiation through metabolic regulation, and suggest that VHL is required for normal kidney development. (lww.com)
  • Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
  • We first optimized a serum-free, miniature erythroid differentiation system starting from primary human CD34 cells, the exact type of cells we would ultimately like to target in vivo ( Figure 1 ). (haematologica.org)
  • In the developing telencephalon, the endothelial expression of P-gp, and the subcellular localization of the transporter at the luminal endothelial cell (EC) plasma membrane are early hallmarks of blood-brain barrier (BBB) differentiation and suggest a functional BBB activity that may complement the placental barrier function and the expression of P-gp at the blood-placental interface. (current-pharmaceutical-design.com)
  • Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
  • In addition, cells were assessed for multi-differentiation potential. (bvsalud.org)
  • Similarly, the cells showed differentiation ability into all three lineages of cells tested. (bvsalud.org)
  • The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
  • Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
  • This process initially occurs in fetal liver cells and subsequently takes place in the bone marrow of children and adults. (medscape.com)
  • Two-color analysis of the expression of CD117 on mouse bone marrow cells. (bdbiosciences.com)
  • A single-cell suspension of BALB/c bone marrow was simultaneously stained with FITC-conjugated RA3-6B2 (anti-mouse CD45R/B220, Cat. (bdbiosciences.com)
  • 1999). First identified and isolated from the bone marrow (BM), MSCs can now be expanded from a variety of other tissues including adipose tissue (AT), umbilical cord blood (UCB), skin, tendon, muscle, and dental pulp (Im et al. (pdffox.com)
  • Haematopoietic stem cells are created during embryo development and they follow a dynamic choreography of anatomical localisation, migrating from the dorsal aorta where they emerge , to the fetal liver and then to the fetal bone marrow. (ox.ac.uk)
  • Hematopoiesis, a process in which stem and progenitor cells differentiate to form blood cellular components occurs primarily in the bone marrow, but during fetal development it can in rare cases occur in "extramedullary" structures such as the ocular choroid vasculature of the eye. (mun.ca)
  • Here we applied single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq) to over 8,000 human immunophenotypic blood cells from fetal liver and bone marrow. (unibg.it)
  • provide a detailed transcriptional and chromatin accessibility map of fetal liver and bone marrow hematopoietic stem cells (HSCs). (unibg.it)
  • In view of the fact that UCB has become a valuable, non-invasive source of stem cells for regenerative therapy, establishment of a public cord blood bank (CBB) in South Africa would vastly improve the availability of haematopoietic stem cells for research and therapeutic uses, and increase the tissue genetic diversity that currently impedes the South African bone marrow registry. (sajbl.org.za)
  • These progenitor cells have greater clonal expansion and proliferative capacity than normal bone marrow cells. (sajbl.org.za)
  • 2 , 3 Normally, the bone marrow serves as the source for replenishing the cellular components of peripheral blood including red and white blood cells and platelets. (sajbl.org.za)
  • Experimental and human studies with intravitreal bone marrow-derived stem cells have begun. (biomedcentral.com)
  • Recent work using primary human foetal liver and bone marrow cells, human embryonic stem cells and iPS cells shows that prior to acquisition of GATA1 mutations, trisomy 21 itself alters human foetal haematopoietic stem cell and progenitor cell biology causing multiple abnormalities in myelopoiesis and B-lymphopoiesis. (ox.ac.uk)
  • Red blood cells, most white blood cells, and platelets are produced in the bone marrow, the soft fatty tissue inside bone cavities. (health-innovations.org)
  • Within the bone marrow, all blood cells originate from a single type of stem cell. (health-innovations.org)
  • In contrast, data findings show that few oligopotent progenitor intermediates were present in the adult bone marrow, instead only two progenitor classes predominate, multipotent and unipotent, with erythroid-megakaryocytic lineages emerging from multipotent cells. (health-innovations.org)
  • Progenitor cell (stem cell) lines in the bone marrow produce new blood cells and stromal cells. (medscape.com)
  • Bone marrow consists of stem cells, which are large, "primitive," undifferentiated cells supported by fibrous tissue called stroma. (medscape.com)
  • There are 2 main types of stem cells and, therefore, the bone marrow consists of 2 types of cellular tissue. (medscape.com)
  • Both types of bone marrow are highly vascular, being enriched with numerous blood vessels and capillaries. (medscape.com)
  • Bone marrow first appears in the clavicle near the end of fetal life and becomes active about 3 weeks later. (medscape.com)
  • Bone marrow thus contains blood cells at varying stages of development. (medscape.com)
  • Illustration of the pelvis to show the site of bone marrow and blood cells derived from bone marrow. (medscape.com)
  • When the oxygen content of body tissues is low, if there is loss of blood or anemia, or if the number of red blood cells decreases, the kidneys produce and release erythropoietin, a hormone that stimulates the bone marrow to produce more red blood cells. (medscape.com)
  • Similarly, the bone marrow produces and releases more white blood cells in response to infections, and it produces and releases more platelets in response to bleeding. (medscape.com)
  • If a person experiences serious blood loss, yellow bone marrow can be activated and transformed into red bone marrow. (medscape.com)
  • As age progresses, more of the red bone marrow turns into yellow bone marrow and the production of new blood cells becomes more difficult. (medscape.com)
  • The bone marrow stroma contains mesenchymal stem cells. (medscape.com)
  • Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
  • Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)
  • Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)
  • Low-power view of hematoxylin-eosin-stained bone marrow showing hypocellularity, with increased adipose tissue and decreased hematopoietic cells in the marrow space. (medscape.com)
  • The clinical presentation of patients with aplastic anemia includes signs and symptoms related to the decrease in bone marrow production of hematopoietic cells. (medscape.com)
  • 15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
  • Stem cell-independent pathways during development thus offer a rational strategy for the rapid and simultaneous growth of tissues and stem cell pools. (elsevierpure.com)
  • Introduction Mesenchymal stromal cells (MSCs) are adult, fibroblast-like multipotent cells characterized by the ability to differentiate into tissues of mesodermal origin, such as adipocytes, chondroblasts, and osteoblasts (Friedenstein et al. (pdffox.com)
  • Previous studies have demonstrated that MSCs overexpressing Chemokine (C-X-C motif) receptor 4 (CXCR4) could increase the quantity of transplanted cells to local tissues. (hindawi.com)
  • It has been observed that mesenchymal stem cells (MSCs) could home and engraft within the injured tissues and differentiate into vascular cells and alveolar cells to regenerate the damage in the irradiated mouse lung [ 10 ]. (hindawi.com)
  • In these studies, cells are either removed and edited in tissue culture and then readministered to the patient (ex vivo), or genome editors are packaged within viral vectors or lipid nanoparticles and given intravenously to home to specific tissues (in vivo). (cdc.gov)
  • We report the presence in fetal tissues of 2 distinct CD19+ B-progenitors, an adult-type CD10+ve ProB-progenitor and a new CD10-ve PreProB-progenitor, and describe their molecular and functional characteristics. (ox.ac.uk)
  • Amy Radunz, a nutritionist from the department of animal sciences at the University of Wisconsin in River Falls, has partnered with molecular biologist Hasan Khatib, an associate professor at University of Wisconsin-Madison, to ask how differences in maternal diet during pregnancy induce gene expression and DNA methylation changes in fetal tissues in sheep. (genengnews.com)
  • On day 130 of pregnancy, nonsurvival surgery was performed on the ewes to enable the measure of uterine blood flow to monitor nutrient supply to the fetus and to collect tissue samples from four fetal tissues. (genengnews.com)
  • The study also looked at the impact of paternally expressed genes: IGF2, PEG1, PEG3, DLK1, and DIO3 for transcriptomic and epigenomic alterations of the imprinted genes in fetal tissues. (genengnews.com)
  • HCT/Ps such as amnion/chorion hematopoietic stem/progenitor cells from cord blood and peripheral blood, and reproductive tissues such as semen and oocytes, have the potential for harboring Zika virus. (cdc.gov)
  • Over the last years, dentistry has been exploring the potential application of stem cells from different origins in the regeneration of oral tissues that were lost or damaged by disease or trauma 1 . (bvsalud.org)
  • Blood production during foetal development involves separate waves of migration of rare stem cells among different organs, including aorta, liver and bones. (cam.ac.uk)
  • Through kinetic study, we observed the simultaneous formation of HSCs and defined progenitors-previously regarded as descendants of HSCs 5 -from the HLF + precursor population, followed by prompt formation of the hierarchical haematopoietic population structure in the fetal liver in an HSC-independent manner. (elsevierpure.com)
  • Results show that fetal liver contained large numbers of distinct oligopotent progenitors with intermingled myeloid, erythroid, and megakaryocytic fates. (health-innovations.org)
  • transferrin can transport iron from cells (intestinal, macrophages) to specific receptors on erythroblasts, placental cells, and liver cells. (msdmanuals.com)
  • It is still unclear how human foetal blood stem cells arise, expand and differentiate in the more than ten cell types that carry out vital body functions such as carrying oxygen, clotting and fighting infections. (cam.ac.uk)
  • Normal erythropoiesis begins with multipotent hematopoietic stem cells, which differentiate into erythroid progenitors, eventually to develop into the mature red blood cells. (medscape.com)
  • Proteins are the machinery of the cell, responsible for executing the functions essential for cells to operate, survive, differentiate and divide. (lu.se)
  • Hematopoietic stem cells (HSCs) were first identified in 1961, and their ability to migrate and differentiate into multiple cell types was documented [ 2 ]. (biomedcentral.com)
  • A progenitor cell is a biological cell that, like an SC, has a tendency to differentiate into a specific type of cell but is already more specific than an SC and is pushed to differentiate into its 'target' cell. (biomedcentral.com)
  • As needed, the stem cells differentiate to become a particular kind of cell-a white blood cell, red blood cell, or platelet. (medscape.com)
  • T lymphocytes originate via lymphoid stem cells that migrate to the thymus and differentiate under the influence of the thymic hormones thymopoietin and thymosin. (medscape.com)
  • proposed the "mononuclear phagocyte system" theory, by which tissue-resident macrophages were assumed to derive from blood-circulating monocytes and to differentiate within the host tissue ( 1 ). (frontiersin.org)
  • Erythropoiesis from human embryonic stem cells through erythropoietin-independent AKT signaling. (ca.gov)
  • The goal of the present study was to investigate the potential of ic-MPL dimerization to induce erythropoiesis from human embryonic stem cells (hESC) and to identify the signaling pathways activated by this strategy. (ca.gov)
  • Although less controversial than the use of embryonic stem cells obtained from fetal tissue, the practice of UCB biobanking presents several ethical and regulatory challenges surrounding its procurement and use, especially in developing countries like South Africa, where the majority of the population is vulnerable and prone to exploitation. (sajbl.org.za)
  • As a source of non-embryonic stem cells, UCB has found potential uses as a therapeutic modality in more than 80 clinical applications, both haematological and non-haematological. (sajbl.org.za)
  • Our study provides a useful framework for future investigation of human developmental hematopoiesis in the context of blood pathologies and regenerative medicine. (unibg.it)
  • The broad interest of our group lies in understanding the molecular differences between fetal and adult hematopoiesis . (lu.se)
  • By using in-depth mass spectrometry-based quantitative proteomics to monitor protein levels in a comprehensive and quantitative manner, our ongoing research aims at defining the fetal- and adult-specific proteomic programs that regulate normal and malignant hematopoiesis. (lu.se)
  • Future work will include detailed proteomic profiling of ontogeny-specific leukemic features, investigation of the mechanistic details of hematopoietic stem cell plasticity, as well as specific types of post-translational modifications of particular importance in early hematopoiesis. (lu.se)
  • In my research, I utilise a variety of technologies, including single-cell RNA sequencing, whole genome sequencing and cell-culture assays, to characterise foetal blood progenitors and to understand how stem cells colonise the different organs during development. (cam.ac.uk)
  • This ability of MSCs to adopt a different phenotype in response to sensing an inflammatory environment is not captured in assays that are commonly used to characterize these cells, but it is crucial for understanding their therapeutic potential in immune-mediated disorders. (pdffox.com)
  • In brief, my research team uses cutting edge imaging, clonal tracking (cellular barcoding), genomics technologies, and functional assays (in vivo and in vitro) to tackle long-standing questions about the biology of blood production. (edu.au)
  • Single-cell transcriptomics and functional assays place fetal PreProB-progenitors upstream of ProB-progenitors, identifying them as the first B-lymphoid-restricted progenitor in human fetal life. (ox.ac.uk)
  • Culture conditions of CD34(+) cells - either with or without mesenchymal stromal cells (MSCs) - had relatively little impact on DNAm, although proliferation is greatly increased by stromal support. (nih.gov)
  • In addition to their stem/progenitor properties, mesenchymal stromal cells (MSCs) possess broad immunoregulatory properties that are being investigated for potential clinical application in treating immune-based disorders. (pdffox.com)
  • In response to this challenge, the International Society for Cellular Therapy formulated minimal criteria for defining MSCs in order to create a broader consensus for more uniform characterization of these cells (Dominici et al. (pdffox.com)
  • 2007). In addition to their stem/progenitor properties, MSCs have also been shown to possess broad immunoregulatory abilities and are capable of influencing both adaptive and innate immune responses. (pdffox.com)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • Less quantity of transplanted mesenchymal stem cells (MSCs) influences the therapeutic effects on radiation-induced lung injury (RILI). (hindawi.com)
  • Mesenchymal stem cells (MSCs) have been suggested as an appropriate source for diabetes cell-based therapies. (biomedcentral.com)
  • This study is the first to compare the neuroprotective efficacy ofUCB cells versus MSCs for inflammation-induced preterm braininjury. (brain-injury-hope.com)
  • Both UCB cells and MSCs have protective benefits for thepreterm brain, but their effects on white matter are different.MSCs were strongly anti-inflammatory, dampening multipleindices of brain inflammation at the cellular and gene level. (brain-injury-hope.com)
  • These differential effects of UCB and MSCs are likely dueto specific actions of an isolated cell population (MSCs) versus astem/progenitor cell mix (UCB). (brain-injury-hope.com)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • All stem cells are undifferentiated cells that exhibit unlimited self-renewal and can generate multiple cell lineages or more restricted progenitor populations that can contribute to tissue homeostasis by replenishing the cells or to tissue regeneration after injury. (biomedcentral.com)
  • Four years ago, when they isolated the pure stem cell, the researchers realized they had also uncovered populations of stem-cell like 'daughter' cells that they presumed were other types of stem cells. (health-innovations.org)
  • Results show that in redefining the architecture of blood development, the lineage potential of nearly 3,000 single cells were mapped from 33 different cell populations of stem and progenitor cells obtained from human blood samples taken at various life stages and ages. (health-innovations.org)
  • Areas of research focus are beta cell replacement therapies for the treatment of diabetes, the identification of lung progenitor cell populations involved in lung repair, and in the diagnosis of pancreatic cancer. (ohsu.edu)
  • and 3) facilitate the purification of cell culture expanded cell populations for cell-based therapies. (ohsu.edu)
  • The tremendous ability of pluripotent stem cells (PSC) to generate diverse cell types has led to the possibility that these cells may one day be used as an inexhaustible supply of cells and tissue for therapy. (ca.gov)
  • They go on to add that there are also promising implications for advancing the global quest in regenerative medicine to manufacture mature cell types such as platelets or red blood cells by engineering cells, a process known as inducing pluripotent stem cells. (health-innovations.org)
  • by combining the ability to optimize induced pluripotent stem cells with these newly identified progenitors that give rise only to platelets and red blood cell, they will be able to develop better methods to generate these mature cells. (health-innovations.org)
  • To deliver on that promise, our laboratory is collaborating with the laboratories of Markus Grompe, M.D., OHSU, and Gordon Keller, Ph.D., University Health Network, Toronto, Canada, in the development and characterization of monoclonal antibodies directed against cell surface molecules expressed by pancreatic cell subsets as well as pancreatic progenitor cells derived from pluripotent stem cells. (ohsu.edu)
  • These findings identify emperipolesis as a new cell-in-cell interaction that enables neutrophils and potentially other cells passing through the megakaryocyte cytoplasm to modulate the production and membrane content of platelets. (elifesciences.org)
  • These stem cells divide to eventually give rise to red blood cells, platelets, and most white blood cells in the red marrow. (medscape.com)
  • White blood cells last anywhere from a few hours to a few days, platelets for about 10 days, and red blood cells for about 120 days. (medscape.com)
  • In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
  • The high frequency of a unique neonatal preleukaemic syndrome, transient abnormal myelopoiesis (TAM), and subsequent acute myeloid leukaemia in early childhood in patients with trisomy 21 (Down syndrome) points to a specific role for trisomy 21 in transforming foetal haematopoietic cells. (ox.ac.uk)
  • To achieve this the lab developed a cell-sorting scheme to resolve myeloid, erythroid, and megakaryocytic fates from single CD34+ cells and then mapped the progenitor hierarchy across human development. (health-innovations.org)
  • The common stem cell produces 2 other stem cells, the myeloid stem cell and the lymphoid stem cell. (medscape.com)
  • This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
  • However, prior to commitment, it has been cells, it is evident that GATA-1 and PU.1 are able to specify observed that many genes are expressed at intermediate or basal erythroid and myeloid cell fates (see [16] and references therein). (lu.se)
  • The expansion of HSPCs was evaluated through the total number of UCB mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34(+) phenotypic analysis with flow cytometry. (ox.ac.uk)
  • In 2003 he became Director of the OHSU Hematopoietic Cell Processing Laboratory, and in 2004 he became a member of the Oregon Stem Cell Center, where he is Director of the Center's monoclonal antibody and flow cytometry core facilities and conducts independent research. (ohsu.edu)
  • The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
  • Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
  • D) Representative flow cytometry plots of cells stained with fluorescein isothiocyanate (FITC)-conjugated anti-CD71 and phycoerythrin (PE)-conjugated anti-CD235a antibodies, demonstrating sequential expression of CD71 followed by CD235a and subsequent loss of CD71. (haematologica.org)
  • Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)
  • Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
  • Hematopoietic stem and progenitor cells (HPCs) can be maintained in vitro, but the vast majority of their progeny loses stemness during culture. (nih.gov)
  • By transducing two genes (c-MYC and BCL-XL) into iPS cells and ES cells, a research team has succeeded in producing erythrocyte progenitor cells with almost unlimited ability to replicate in vitro, which they then differentiated successfully into mature erythrocytes. (sciencedaily.com)
  • As with their fetal counterparts, adult BM PreProB-progenitors give rise only to B-lineage cells in vitro and express the expected B-lineage gene expression program. (ox.ac.uk)
  • Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
  • The protein hemoglobin, found in red blood cells, is responsible for oxygen delivery. (medscape.com)
  • In addition, the red cells produced are of an immature type, expressing mostly embryonic rather than adult hemoglobin. (ca.gov)
  • Although these erythrocytes consisted mostly of fetal-type hemoglobin, they were confirmed to have oxygen-carrying capacity and to have circulatory capacity following transfusion into mice. (sciencedaily.com)
  • Potent and uniform fetal hemoglobin induction via base editing. (cdc.gov)
  • Inducing fetal hemoglobin (HbF) in red blood cells can alleviate ß-thalassemia and sickle cell disease. (cdc.gov)
  • Overview of Decreased Erythropoiesis Anemia, a decrease in the number of red blood cells (RBCs), hemoglobin (Hb) content, or hematocrit (Hct), can result from decreased RBC production (erythropoiesis), increased RBC destruction. (msdmanuals.com)
  • Although the anemia is often normocytic, mild macrocytosis can also be observed in association with stress erythropoiesis and elevated fetal hemoglobin levels. (medscape.com)
  • Red cell development, or erythropoiesis, is a carefully ordered sequence of events. (medscape.com)
  • Current methods to induce erythropoiesis from PSC suffer from low yields of RBCs, most of which are immature and contain embryonic and fetal rather than adult hemoglobins. (ca.gov)
  • We have previously shown that homo-dimerization of the intracellular component of MPL (ic-MPL) induces erythropoiesis from human cord blood progenitors. (ca.gov)
  • 1 The accumulation of free excess α-globin chains in red blood cells and their precursors, as a result of the decreased production of β-globin, is believed to be the main pathophysiological mechanism leading to hemolytic anemia and ineffective erythropoiesis in β-thalassemia. (haematologica.org)
  • These studies reveal potentially new therapeutic targets for recovery from anemia and regeneration of blood cells for transplantation. (ca.gov)
  • Recent medical advances in the field of regenerative medicine and tissue transplantation have highlighted the importance of umbilical cord blood (UCB) as a valuable alternative source of haematopoietic stem cells, which are potentially life-saving in a vast array of clinical applications. (sajbl.org.za)
  • BACKGROUND: The Cord Blood Transplantation (COBLT) Study banking program was initiated in 1996. (emmes.com)
  • The study goals were to develop standard operating procedures for cord blood (CB) donor recruitment and banking and to build an ethnically diverse unrelated CB bank to support a transplantation protocol. (emmes.com)
  • Our limited understanding of the complex cellular composition, anatomy, and function of this tissue has slowed the establishment of effective tissue regenerative (repair) and/or cell replacement (transplantation) therapies. (ohsu.edu)
  • Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
  • Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
  • The specific medications administered depend on the choice of therapy and whether it is supportive care only, immunosuppressive therapy, or hematopoietic cell transplantation. (medscape.com)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • Placental trophoblasts are a major cell type of placenta and play important roles in many critical processes during pregnancy, including implantation, hormone production and regulation, immune protection of the fetus, and nutrient supply. (rochester.edu)
  • In the novel and exciting field of regenerative medicine, umbilical cord blood (UCB), also known as placental blood, is no longer considered biological waste. (sajbl.org.za)
  • Additionally, cell salvage has proven beneficial and should be considered for various indications in obstetrics including hematologic disturbances, as well as various forms of placental disorders. (karger.com)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
  • Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
  • Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
  • As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)
  • In early fetal ages, P-gp has also been immunolocalized on radial glia cells (RGCs), located in the proliferative ventricular zone (VZ) of the dorsal telencephalon and now considered to be neural progenitor cells (NPCs). (current-pharmaceutical-design.com)
  • Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)
  • In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
  • Two types of white blood cells, T and B cells (lymphocytes), are also produced in the lymph nodes and spleen, and T cells are produced and mature in the thymus gland. (health-innovations.org)
  • Cord blood was analyzed using a FACSort flow cytometer to determine proportions of T lymphocytes (CD3 + cells and their subsets, CD4 + and CD8 + ), B lymphocytes (CD19 + ) and natural killer (NK) cells. (biomedcentral.com)
  • Multivariable linear regression models were fitted to estimate associations between monthly PAH or PM 2.5 and cord blood lymphocytes, adjusting for year of birth and district of residence and, in further models, gestational season and number of prior live births. (biomedcentral.com)
  • The adjusted models show significant associations between PAHs or PM 2.5 during early gestation and increases in CD3 + and CD4 + lymphocytes percentages and decreases in CD19 + and NK cell percentages in cord blood. (biomedcentral.com)
  • PAHs and PM 2.5 in ambient air may influence fetal immune development via shifts in cord blood lymphocytes distributions. (biomedcentral.com)
  • These data suggest that fetal HSCs minimally contribute to the generation of progenitors and functional blood cells before birth. (elsevierpure.com)
  • Data from two pivotal trials suggest that a single infusion of the CRISPR-based gene therapy exagamglogene autotemcel (exa-cel) can provide a 'functional cure' for patients with transfusion-dependent beta-thalassemia or severe sickle cell disease. (cdc.gov)
  • Nephron progenitors, the cell population that give rise to the functional unit of the kidney, are metabolically active and self-renew under glycolytic conditions. (lww.com)
  • These may be attributed to the changes in the functional status of stem cells. (ijbs.com)
  • The expression of CD117 has been included in protocols to isolate hematopoietic progenitors from fetal and adult peripheral blood. (bdbiosciences.com)
  • genes in peripheral blood of at-risk, new-onset and long-term type 1 DM , and healthy controls. (indexindex.com)
  • Human CD34 + hematopoietic stem and progenitor cells purified from umbilical cord or adult peripheral blood were cultured in a two-phase liquid culture system in a serum-free medium for 21 days. (haematologica.org)
  • However, fetal PreProB-progenitors display a distinct, ontogeny-related gene expression pattern that is not seen in adult PreProB-progenitors, and they share transcriptomic signatures with CD10-ve B-progenitor infant acute lymphoblastic leukemia blast cells. (ox.ac.uk)
  • Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition. (ox.ac.uk)
  • The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated osteoblasts under hypoxia environment was investigated. (ox.ac.uk)
  • What is umbilical cord blood banking and why is it important? (sajbl.org.za)
  • 1 , 2 UCB is the blood in the cut umbilical cord and placenta after delivery. (sajbl.org.za)
  • In the present study, we conducted overexpressing CXCR4 human umbilical cord mesenchymal stem cell (HUMSC) therapy for RILI. (hindawi.com)
  • Foetal stem cells have a higher regenerative potential than their adult counterparts and studying them could provide important insights on how we can mimic these properties in the production of blood stem cells ex-vivo for regenerative medicine purposes. (cam.ac.uk)
  • Here we used in vivo genetic tracing in mice to analyse the formation of HSCs and progenitors from intra-arterial haematopoietic clusters, which contain HSC precursors and express the transcription factor hepatic leukaemia factor (HLF). (elsevierpure.com)
  • By genetically manipulating EVI1 expression, we were able to alter HSC and progenitor output from precursors in vivo. (elsevierpure.com)
  • Hopes for the creation of a more stable transfusion system have centered on the establishment of a technology for large-scale erythrocyte production ex vivo, using for instance iPS cells or ES cells. (sciencedaily.com)
  • Ex vivo therapies have focused on blood disorders, including at least 6 trials aiming to cure sickle cell disease (SCD) or transfusion-dependent ß-thalassemia (TDT). (cdc.gov)
  • AKT activation upregulates the GATA-1 and FOXO3 transcriptional pathways with resulting inhibition of apoptosis, modulation of cell cycle and enhanced maturation of erythroid cells. (ca.gov)
  • It was also found that the expression of the BCL-XL gene was higher during the maturation process of erythrocyte progenitor cells than during their replication process. (sciencedaily.com)
  • By embryonic day 15.5, kidneys of nephron progenitor cell-specific VHL knockout mice begin to exhibit reduced maturation of nephron progenitors. (lww.com)
  • Although fetal BM PreProB-progenitors and ProB-progenitors both give rise solely to B-lineage cells, they are transcriptionally distinct. (ox.ac.uk)
  • The focus was on the effects of fetal bovine serum (FBS) replacement with pooled human serum (HS). (biomedcentral.com)
  • The cellular fraction was processed and frozen with L-proline (1.5M) + trehalose (0.2M) or with DMSO + fetal bovine serum (FBS) as control. (bvsalud.org)
  • Evaluating Interaction of Cord Blood Hematopoietic Stem/Progenitor Cells with Functionally Integrated Three-Dimensional Microenvironments. (wakehealth.edu)
  • This study investigates the association between cord blood lymphocyte proportions and maternal exposure to air pollution during each gestational month. (biomedcentral.com)
  • Given that lymphocyte production, including T and B cell development, starts early in gestation [ 7 ] and that critical stages in development of the immune system may also reflect temporal variation in susceptibility to immunotoxicants, this study investigated the association between maternal exposure to air pollution during each month of gestation and cord blood lymphocyte proportions. (biomedcentral.com)
  • 1) « behavioural deficit was significantly improved with multiple doses of cord blood, but not with a single dose. (brain-injury-hope.com)
  • We also showed that the injury caused significant loss of brain tissue and cell death, which was only improved in the study arm that administered multiple doses of cord blood cells. (brain-injury-hope.com)
  • At the end of 7 days of culture, the UCB-MNCs, CFU-Cs and CD34(+) cells had achieved 18.7 +/- 1.6, 11.6 +/- 0.9 and 23.4 +/- 2-fold expansions, respectively, in the test groups. (ox.ac.uk)
  • Our research has shown that aldehydes from maternal alcohol consumption or from metabolism can damage the DNA of the stem cells. (ox.ac.uk)
  • The aim of this project is to better understand how maternal metabolism and DNA damage repair and the DNA damage response shape early haematopoietic stem cell instruction, survival and dysfunction. (ox.ac.uk)
  • The cells actively release extracellular vesicles (EVs), nano-sized (.05-1 μm) membrane-bound vesicles, into the maternal and the fetal circulation. (rochester.edu)
  • The working hypothesis going into the experiment was that the primary impact on the fetal growth in previous studies was the result of the amount of glucose in the maternal diet. (genengnews.com)
  • Pregnancy, delivery, and the puerperium are associated with increased rates of iron deficiency and anemia, which correlates with worse maternal and fetal outcomes and places pregnant women at increased risk of obstetric hemorrhage. (karger.com)
  • MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
  • One type of stem cell is involved in producing blood cells and the other is involved in producing stromal cells, which are responsible for the supporting stroma. (medscape.com)
  • The transcription factor EVI1 is heterogeneously expressed within the precursor population, with EVI1 hi cells being predominantly localized to intra-embryonic arteries and preferentially giving rise to HSCs. (elsevierpure.com)
  • Using fate tracking, we also demonstrated that fetal HSCs are slowly used to produce short-term HSCs at late gestation. (elsevierpure.com)
  • 2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
  • The regulation of mammalian cell volume is crucial for maintaining key cellular processes. (bvsalud.org)
  • Increasing evidence emphasize that a substantial variance of protein expression is conveyed at the levels of protein synthesis and degradation, and that post-transcriptional control of gene expression plays a critical role in multiple aspects of cell biology, including coordination of cellular processes and rapid alteration of cellular phenotype. (lu.se)
  • In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
  • These data identify PreProB-progenitors as the earliest B-lymphoid-restricted progenitor in human fetal life and suggest that this fetal-restricted committed B-progenitor might provide a permissive cellular context for prenatal B-progenitor leukemia initiation. (ox.ac.uk)
  • Single-cell omics provide insight into cellular heterogeneity and function. (nature.com)
  • Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
  • The molecular basis by which trisomy 21 exerts these effects is likely to be extremely complex, to be tissue-specific and lineage-specific and to be dependent on ontogeny-related characteristics of the foetal microenvironment. (ox.ac.uk)
  • Our laboratory, in collaboration with the laboratory of Jeffrey Whitsett, M.D., at the Cincinnati Children's Hospital, is generating and characterizing a panel of monoclonal antibodies targeting cell-surface markers on different subsets of pulmonary cells involved in the process of pulmonary tissue regeneration. (ohsu.edu)
  • They are often studied independently from other tissue-resident macrophages, probably because they are unique in some aspects, most notably in their isolation from the rest of the body through the blood brain barrier (BBB). (frontiersin.org)
  • Materials and method: The pulp tissue, obtained from wisdom teeth, was placed in a 6-well plate containing proper culture medium, and stored at 37 °C and 5% CO2 for cell proliferation and plastic adherence. (bvsalud.org)
  • Given this, the ALINE MANICA2 cryopreservation of adipose tissue-derived mesenchymal stem cells (ADMSCs) could be a JULIANA CRISTINA SCHMIDT2 way to collect them in one surgical procedure and after performing fractional fat grafting. (bvsalud.org)
  • Dinorah Friedmann-Morvinski, a post-doctoral fellow in Inder Verma's lab at the Salk Institute, has shown how tumors originated from mature neurons dedifferentiate into glioma stem-like progenitor cells. (genengnews.com)
  • Instead, tumor perivascular astrocytes may dedifferentiate and resume a progenitor-like P-gp activity, becoming MDR cells and contribute, together with perivascular P-gpexpressing glioma stem-like cells (GSCs), to the MDR profile of GBM vessels. (current-pharmaceutical-design.com)
  • In our recent study, we examined the impact of the three-dimensional co-culture of FPMSCs on human embryonic stem cell-derived pancreatic progenitors' development. (biomedcentral.com)
  • One recently developed reagent recognizes a molecule associated with human pancreatic cancer cells as well as precancerous lesions of the pancreas. (ohsu.edu)
  • EVs shuttle cargoes of bioactive molecules, such as proteins, lipids, and nucleic acids, from trophoblasts to the recipient cells, modifying gene expression and biology in the cells. (rochester.edu)
  • This project focuses on the compelling need to generate cell-surface marker specific reagents useful for identification, study, and isolation of pulmonary stem/progenitor cells. (ohsu.edu)
  • Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. (nature.com)
  • Conclusion: Our results suggest that the explant method - or cell proliferation method - is suitable for the isolation and culture of stem cells from dental pulp of permanent teeth. (bvsalud.org)
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
  • To study the potential role in this metabolic shift of von Hippel-Lindau (VHL), a protein component of a ubiquitin ligase complex, the authors generated nephron progenitor cell-specific VHL knockout mice. (lww.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • Mechanism of early susceptibility is not fully understood, but may be explained, in part, by environmental impact on neural stem cells (NSCs). (rochester.edu)
  • Growing evidence indicates that EVs reach fetal neural cells after crossing the blood-brain barrier (BBB). (rochester.edu)
  • I hypothesize that genes or microRNAs, which are identified as functionally important in neural stem cell function, will have genetic polymorphisms that associate with metal-impaired neurodevelopment in susceptible children. (rochester.edu)
  • One common denominator of these conditions is progressive loss of the neural cells of the eye - photoreceptors, interneurons, and retinal ganglion cells, or RGCs - and essential supporting cells such as the retinal pigment epithelium (RPE). (biomedcentral.com)
  • RG-like NPCs have been found in many regions of the developing brain and have been suggested to give rise to neural stem cells (NSCs) of adult subventricular (SVZ) neurogenic niches. (current-pharmaceutical-design.com)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • Discovery of a CD10-negative B-progenitor in human fetal life identifies unique ontogeny-related developmental programs. (ox.ac.uk)
  • Blood Cancer Discovery Sep 9;2(6):586-599, 2021. (stjude.org)
  • Human PSC can produce red cells in culture but the process is currently inefficient producing too few cells for practical use. (ca.gov)
  • As the c-MYC gene and BCL-XL gene are present within erythrocyte progenitor cells, methods that make use of them actually mimic the body's own system and can therefore be thought of as safer with a view to future clinical application than the previously developed systems for large-scale erythrocyte production using oncogene-derived from Human papillomavirus that are not normally expressed in the human body. (sciencedaily.com)
  • Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
  • The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
  • Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
  • In human blood formation, everything begins with the stem cell , which is the executive decision-maker quickly driving the process that replenishes blood at a daily rate that exceeds 300 billion cells. (health-innovations.org)
  • Now, a study from researchers at the University of Toronto have discovered a completely new view of how human blood is made, upending the conventional doctrine from the 1960s. (health-innovations.org)
  • Earlier studies from the group isolated a human blood stem cell in its purest form, as a single stem cell capable of regenerating the entire blood system. (health-innovations.org)
  • Data findings show that the blood system is two-tiered and changes between early human development and adulthood. (health-innovations.org)
  • The lab state that their discovery means the global medical community will be able to understand a wide variety of human blood disorders and diseases far better, from anemia, where there are not enough blood cells, to leukemia, where there are too many blood cells. (health-innovations.org)
  • Here, we provide a comprehensive analysis of the human fetal B-cell developmental hierarchy. (ox.ac.uk)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • In 2011, I started my own laboratory at WEHI with a consistently innovative research program focused on the biology of blood production and function. (edu.au)
  • Frontiers in cell and developmental biology. (lu.se)
  • Splicing factor deficits render hematopoietic stem and progenitor cells sensitive to STAT3 inhibition. (stjude.org)
  • However, when the team burrowed further to study these 'daughters', they discovered they were actually already mature blood lineages. (health-innovations.org)
  • In other words, lineages that had broken off almost immediately from the stem cell compartment and had not developed downstream through the slow, gradual 'textbook' process. (health-innovations.org)