Progenitor cell biology. Medical search. Frequent questions

Timely differentiation of progenitor cells is critical for development. (rupress.org)
In this study we asked whether global epigenetic mechanisms regulate timing of progenitor cell differentiation into myelin-forming oligodendrocytes in vivo. (rupress.org)
During the first 10 postnatal days, administration of valproic acid (VPA), the specific inhibitor for histone deacetylase activity, resulted in significant hypomyelination with delayed expression of late differentiation markers and retained expression of progenitor markers. (rupress.org)
The concept of timing of OL differentiation was originally proposed based on very elegant studies on cultured progenitors purified from the optic nerve ( Temple and Raff, 1986 ). (rupress.org)
This hypothesis implied that OL differentiation proceeded by default once the cells exited from the cell cycle. (rupress.org)
It is an important tool that will allow scientists to study cell renewal and differentiation in human cells, perhaps offering clues to how to regulate such development," added first author Stefano Da Sacco. (biologynews.net)
Neural Progenitor Cells Derived from XCL-1 DCXp-GFP are a neuronal lineage reporter cell line that can be used in neuronal differentiation and drug screening. (atcc.org)
Phenotype switching: plasticity and/or differentiation of stromal cells and their progenitors within the tumour microenvironment regulate tumour fate. (europa.eu)
Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
These cells enable us to study the biochemical changes occurring in the differentiation process of an erythroid progenitor cell induced by the hormone erythropoietin. (silverchair.com)
The developmental strategies used by progenitor cells to allow a safe journey from their induction place towards the site of terminal differentiation are still poorly understood. (elifesciences.org)
The remaining delaminated cells follow the movement of apically attached progenitors by a protrusion-dependent cell-cell contact mechanism, avoiding sequestration by the adjacent endoderm, ensuring their collective fate and allocation at the site of differentiation. (elifesciences.org)
These early progenitor cells often travel long distances from their induction site to the site of terminal differentiation, making them vulnerable to environmental cues and movement of neighbouring tissues. (elifesciences.org)
When faced with these environmental hazards, progenitors may deviate from their correct pathway or change their potential, leading to a reduction in the pool of progenitors available for later stages of differentiation. (elifesciences.org)
Despite the importance of the developmental pathways followed by these small groups of progenitor cells and their impact on the physiology of the organism, we still know little about the set of developmental strategies that progenitor cells deploy in vivo to overcome the challenges imposed by the environment as they travel to the site of terminal differentiation. (elifesciences.org)
Genetic studies have shown that formation of pancreatic endocrine cells in mice is dependent on the cell autonomous action of the bHLH transcription factor Neurogenin3 and that the extent and timing of endocrine differentiation is controlled by Notch signaling. (biomedcentral.com)
Cell autonomous inhibition of Notch signal reception results in significantly increased endocrine differentiation demonstrating that these early progenitors are prevented from differentiating by ongoing Notch signaling. (biomedcentral.com)
Lastly, our results demonstrate that expression of differentiation markers can be uncoupled from the process of delamination of differentiating cells from the epithelium. (biomedcentral.com)
Together these studies suggest that Notch signaling prevents endocrine differentiation through a mechanism known as lateral inhibition where the Notch ligand Dll1, expressed in differentiating cells, signals through Notch receptors on adjacent cells thereby keeping them undifferentiated or acquiring a secondary fate. (biomedcentral.com)
Their angiogenic activity is distinct from putative " endothelial progenitor cells " that become structural cells of the endothelium by differentiation into endothelial cells . (bvsalud.org)
Rebar N. Mohammed Hematopoietic stem cells (HSCs) are a rare population of cells that reside mainly in the bone marrow and are capable of generating and fulfilling the entire hematopoietic system upon differentiation. (karger.com)
IL-7 is necessary to specify CD8 lineage differentiation during CD4/CD8 cell fate choice in the thymus by inducing expression of the transcription factor Runx3. (biolegend.com)
Combined signaling through interleukin-7 receptors and Flt3 selectively promotes B-cell commitment and differentiation from uncommitted murine bone marrow progenitor cells. (biolegend.com)
Cells recovered from healthy individuals could provide a model for understanding how to stimulate and guide the normal processes of brain cell growth and differentiation," he said, "lending insight to how growth might be stimulated in people suffering from neurodegenerative diseases such as Alzheimer's or Parkinson's. (salk.edu)
The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
Consequently, investigation of molecular heterogeneity in the differentiation potential of HSPC is vital to identify novel regulators that affect generation of specific cell types, especially RBC. (lu.se)
erythroid cells after in vitro differentiation, despite having similar frequencies of phenotypic HSC initially. (lu.se)
The focus of my research is to understand cell fate specification in the developing brain and in human neural progenitor cells, using cell-based models of neuronal differentiation. (lu.se)
Our current focus is to learn how to direct and efficiently drive controlled differentiation of human stem cells into subtype-specific neurons. (lu.se)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

However, major advances in the study of neural progenitor cells have altered these perceptions, and rational approaches to the repair of the damaged nervous system using transplanted progenitor cells now seem feasible. (northwestern.edu)
This review will discuss the basic biology of neural progenitor cells, the mechanisms regulating the generation of neurons and glia from these cells, and the techniques that are available for preparing such cells for transplantation into the nervous system. (northwestern.edu)
To download a certificate of analysis for Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
The certificate of analysis for that lot of Neural Progenitor Cells Derived from XCL-1 DCXp-GFP ( ACS-5005 ) is not currently available online. (atcc.org)
During human forebrain development, neural progenitor cells (NPCs) in the ventricular zone (VZ) undergo asymmetric cell divisions to produce a self-renewed progenitor cell, maintaining the potential to go through additional rounds of cell divisions, and differentiating daughter cells, populating the developing cortex. (elifesciences.org)
During human brain development, neural progenitor cells (NPCs) undergo two modes of cell division. (elifesciences.org)
The research in our laboratory focuses on understanding the molecular mechanisms that regulate neural stem cells and neurodevelopment with the goal of applying this knowledge in the treatment of neurological disorders and injuries. (wisc.edu)
Neural stem cells in the postnatal brain have significant roles in both normal brain functions, such as learning and memory and the brain's response to injuries. (wisc.edu)
My laboratory is investigating the mechanisms governing the behaviors and functions of neural stem cells in both healthy conditions and in neurological diseases. (wisc.edu)
Using neural stem cells as model systems, we are investigating the molecular mechanisms that regulate neuronal development during postnatal period and their implications in human neurodevelopmental disorders such as Rett Syndrome, Autism, and Fragile X syndrome. (wisc.edu)
Such a cell would be a neural stem cell by a strict definition. (salk.edu)
[ 2 ] As neuroendocrine carcinomas were thought to be of neural crest origin, in 1978 Tang and Toker proposed that this carcinoma might be derived from Merkel cells. (medscape.com)
With the advent of improved immunohistochemical profiling, the tumor was reclassified as Merkel cell carcinoma because shared epithelial and neuroendocrine markers, along with ultrastructural features suggestive of neural crest origin, were observed in both the constituent tumor cell and the physiologic Merkel cell of the skin. (medscape.com)
Merkel cells were thought to be of neural crest origin because they are excitable cells that secrete neuropeptides and stain positively for neuroendocrine markers. (medscape.com)
Other suggested cells of origin include the neural crest‒derived cell of amine precursor uptake and decarboxylation (APUD) system, dermal fibroblasts, pre or pro B cells, residual epidermal stem cells, and epithelial, non-Merkel cell progenitors. (medscape.com)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)

Previous work in the embryonic rodent brain suggested that the preferential inheritance of the pre-existing (older) centrosome to the self-renewed progenitor cell is required to maintain stem cell properties, ensuring proper neurogenesis. (elifesciences.org)
Using a novel, recombination-induced tag exchange-based genetic tool to birthdate and track the segregation of centrosomes over multiple cell divisions in human embryonic stem cell-derived regionalised forebrain organoids, we show the preferential inheritance of the older mother centrosome towards self-renewed NPCs. (elifesciences.org)
Here, we uncovered a mechanism of progenitor cell allocation that stems from an incomplete process of epithelial delamination that allows progenitors to coordinate their movement with adjacent extra-embryonic tissues. (elifesciences.org)
Several embryonic tissues and organs originate from small sets of progenitor cells. (elifesciences.org)
Transcriptional regulation of epithelial and endothelial cell functions during lung embryonic development and lung carcinogenesis. (cincinnatichildrens.org)
Embryonic development occurs with precisely timed morphogenetic cell movements directed by complex gene regulation. (duke.edu)
Two cell lineages are observed on the embryonic-abembryonic axis of the blastocyst: the inner cell mass and the trophectoderm. (bioone.org)
In mouse embryos, cells seem prepatterned to become certain cell lineage because the first cleavage plane has been related with further embryonic-abembryonic axis at the blastocyst stage. (bioone.org)
Blastocysts were then classified according to the allocation of the labeled cells in the embryonic and/or abembryonic part of the blastocyst. (bioone.org)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)

Pancreatic progenitor cells are multipotent stem cells originating from the developing fore-gut endoderm which have the ability to differentiate into the lineage specific progenitors responsible for the developing pancreas. (wikipedia.org)
Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)
In postnatal life , this functional connection between the vasculature and hematopoiesis is maintained by a subset of hematopoietic progenitor cells endowed with the capacity to differentiate into potent proangiogenic cells . (bvsalud.org)
Mesenchymal stem cells (MSCs) are multipotent stem/progenitor cells which can differentiate to several mesodermal derivatives and possess an ability to secrete factors involved in neovascularization and immunomodulation [ 1 , 2 ]. (hindawi.com)
I find it remarkable that we all have pockets of cells in our brains that can grow and differentiate throughout our lives and even after death," said Fred Gage , a professor at The Salk Institute and senior author of the study, which appears in the current Nature. (salk.edu)
Importantly, the recovered cells had the ability to differentiate into different types of brain cells. (salk.edu)
In addition to isolating single cells in order to determine if true stem cells are present in adult brain tissue, Gage also plans to transplant recovered cells into animals to test if the cells can survive and differentiate in vivo. (salk.edu)
Watch the lecture where Malin Parmar tells about the long history of transplantation trials in Lund and how they have been able to differentiate stem cells into specific dopaminergic cells in vitro. (lu.se)
Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)

This variation in the development of islet cell subtypes is due to differential gene expression and induction pathways of progenitor cells. (wikipedia.org)
Progenitor cell therapy may also allow functional reorganization of the auditory pathways including primary auditory cortex (Heschl's gyrus). (intechopen.com)
How are signaling pathways coordinated together so that different organs can be produced from the common foregut progenitor? (cincinnatichildrens.org)
Our research aims to elucidate the molecular basis of lung development and disease with specific focus on identifying critical biologic functions of the Rb/p16, p53 and Dicer/miRNA pathways in pulmonary progenitor/stem cell growth in the context of organogenesis, repair after injury and disease pathogenesis to identify novel targets with diagnostic and therapeutic utility. (cincinnatichildrens.org)
In addition, IL-7 controls T-cell size and metabolism through the activation of PI3 kinase-dependent pathways and regulation of glucose metabolism. (biolegend.com)
Even as the nature of the exact cell in which Merkel cell carcinoma oncogenesis occurs is controversial, there is strong support for the notion that Merkel cell carcinoma results from of one of two distinct pathways. (medscape.com)

Pancreatic progenitor cells have been shown to arise from cells originating from the developing foregut during mammalian development. (wikipedia.org)
These genes regulates the expression of Mnx1 in the ventral bud, leading to the developmental specification of the pancreatic progenitor cells expressing Pdx1. (wikipedia.org)
The differential of pancreatic progenitors from hepatic progenitors is also notable, as Hhex1 (Hematopoietically expressed homeobox gene) is responsible for the origination of pancreatic progenitor cells. (wikipedia.org)

It is expressed on early B lymphoid lineage cells in bone marrow, on primitive myeloid progenitors within the BM CD34+ cell population. (biolegend.com)
Ligation of Flk-2 with Flt3 ligand regulates the growth of hematopoietic stem cells and promotes the survival of primitive hematopoietic progenitor cells with myeloid as well as B lymphoid potential. (biolegend.com)
POT1 mutation carriers had a range of benign and malignant neoplasms involving epithelial, mesenchymal, and neuronal tissues in addition to B- and T-cell lymphoma and myeloid cancers. (cdc.gov)
[ 1 ] ETP-ALL frequently has mutations in RUNX1 and/or ETV6 in addition to genes that are more commonly associated with myeloid neoplasms and are otherwise rare in T-cell lymphoblastic leukemia/lymphoma (such as FLT3 , IDH1/2 , TET2 , and DNMT3A mutations). (medscape.com)

OPC architecture, proliferation and oligodendro-/myelino-genesis are finely regulated by the interplay of cell-intrinsic and extrinsic factors. (nature.com)
Our results unveil a novel player in the complex regulation of OPC biology, on the one hand showing that JNK1 ablation cell-autonomously determines alterations of OPC proliferation and branching architecture and, on the other hand, suggesting that JNK1 signaling in OLs participates in myelination in vivo. (nature.com)
Both a cell-intrinsic program and environmental factors participate in the regulation of OPC architecture, proliferation and oligodendro-/myelino-genesis. (nature.com)
Our results also demonstrate that Notch can repress Ngn3 and NeuroD protein function and stimulate progenitor proliferation. (biomedcentral.com)
Formation of new myonuclei required for efficient muscle hypertrophy relies on prior activation and proliferation of muscle stem cells (MuSCs). (mdc-berlin.de)
Here we demonstrate that interstitial mesenchymal progenitors respond to mechanical load and stimulate MuSC proliferation in a surgical mouse model of increased muscle load. (mdc-berlin.de)
Mechanistically, transcriptional activation of Yes-associated protein 1 (Yap1)/transcriptional coactivator with PDZ-binding motif (Taz) in mesenchymal progenitors results in local production of thrombospondin-1 (Thbs1), which, in turn, drives MuSC proliferation through CD47 signaling. (mdc-berlin.de)
For several decades, neovascularization is believed to be accomplished by proliferation of mature endothelial cells residing in the local vessels through the process of angiogenesis [ 6 ]. (hindawi.com)
In this fashion, IL-7 induces cell activation, survival, and proliferation of T lymphocytes. (biolegend.com)
Moreover, in adults, Merkel cells undergo slow turnover and are replaced by cells originating from epidermal stem cells, not through the proliferation of differentiated Merkel cells. (medscape.com)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)

Biology and flow cytometry of proangiogenic hematopoietic progenitors cells. (bvsalud.org)
These proangiogenic hematopoietic progenitors comprise a specific subset of bone marrow (BM)-derived cells that homes to sites of neovascularization and possess potent paracrine angiogenic activity. (bvsalud.org)
There is emerging evidence that this subpopulation of hematopoietic progenitors plays a critical role in vascular health and disease . (bvsalud.org)

During this process, progenitors retain long-lasting apical contacts that enable the epithelial layer to pull a subset of progenitors on their way to the vegetal pole. (elifesciences.org)

During Central Nervous System ontogenesis, myelinating oligodendrocytes (OLs) arise from highly ramified and proliferative precursors called oligodendrocyte progenitor cells (OPCs). (nature.com)
Myelinating OLs originate from parenchymal precursors expressing the neuron-glia antigen 2 (NG2) chondroitin sulphate proteoglycan and the platelet derived growth factor receptor alpha (PDGFRα), commonly referred to as oligodendrocyte progenitor cells (OPCs) 4 . (nature.com)

Genetic lineage tracing experiments have been performed by various research groups to show that the cell clusters originating from the developing foregut express a transcription factor called PDX1 (Pancreatic and duodenal homeobox 1). (wikipedia.org)
In addition to defining the genetic profile of human NP, this system will facilitate studies of human kidney development, providing a novel tool for renal regeneration and bioengineering purposes," says principal investigator Laura Perin, PhD, co-director of CHLA's GOFARR Laboratory for Organ Regenerative Research and Cell Therapeutics in Urology. (biologynews.net)
Approximately half of congenital SNHL is hereditary and is the result of genetic mutations causing improper development of cochlear hair cells. (intechopen.com)
We will present a summary of the effect of hearing loss on auditory development, existing preclinical and clinical data on progenitor cell therapy, and its potential role in the (re)habilitation of non-genetic SNHL. (intechopen.com)
Thus, the data described here use a novel genetic approach to birthdate centrosomes in human cells and identify asymmetric inheritance of centrosomes as a mechanism to maintain self-renewal properties and to ensure proper neurogenesis in human NPCs. (elifesciences.org)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)

Their results, published online in the journal Stem Cell Translational Medicine , will help scientists understand how these progenitor cells become renal cells in the developing fetus, and possibly offer a future way to foster renal regeneration after chronic kidney failure or acute injury. (biologynews.net)
The loss of a sufficient number of nephrons at any time after this period, leads to irreversible kidney failure, as no further cell repair or regeneration can occur. (biologynews.net)
These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
Following postdoctoral fellowships at the Center for Genomic Regulation (Barcelona, Spain) and the University of Colorado-Boulder, Dr. Doles moved to Mayo Clinic in 2016, where he leads a basic science research lab focused on stem cells, tissue regeneration, and multi-organ wasting. (jove.com)
Arur S , Briscoe J, Helariutta Y, Lecuit T and Roberstson L. Imaging development, stem cells and regeneration. (mdanderson.org)
In the adult, stem cells exist in many tissues throughout life and may play critical roles in physiological functions and tissue regeneration. (wisc.edu)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Here, you will find a collection of insightful interviews with PhD graduates from various fields within stem cell biology and regenerative medicine, each sharing their personal experiences and perspectives on their academic journey. (lu.se)
My group works with translational stem cell biology. (lu.se)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

The findings will be of interest to developmental neurobiologists, but also more broadly to cell and developmental biologists. (elifesciences.org)
My research areas include developmental biology, esophageal arresia, tracheoesophageal fistula and airway malacia. (cincinnatichildrens.org)
Present address: Department of Genetics and Developmental Biology, Institut Curie, 75248 PARIS Cedex 05, France. (biologists.com)

In adult mice, IL-7/IL-7R signaling up regulates expression of early B cell factor (EBF), and EBF in turn regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. (biolegend.com)

basal progenitors. (uni-muenchen.de)
[ 4 ] These specialized sensory cells are normally found in the basal epidermis of skin and parts of mucosal surfaces derived from the ectoderm. (medscape.com)
Within skin, Merkel cells have also been shown to cluster in the basal layers of the interfollicular epidermis in specialized epithelial structures called touch domes, where they are juxtaposed with epidermal keratinocytes. (medscape.com)
[ 6 ] As they are primarily postmitotic, Merkel cells putatively have low sensitivity to oncogenic stimuli, and they are found in the basal epidermis, whereas cutaneous neuroendocrine carcinomas arise in the dermis. (medscape.com)

In humans, about 500,000 to 1,000,000 nephrons are generated before week 34 to 36 of fetal gestation, a point at which NP cells are fully depleted and nephrogenesis ceases. (biologynews.net)
An adult source of stem cells would circumvent the need to derive such cells from fetal tissue, currently an issue of ethical debate. (salk.edu)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

OLs are the myelin-forming cells of the central nervous system and they derive from progenitor cells generated by multipotent precursors. (rupress.org)
[ 7 , 8 ] Sunshine et al offer that perhaps there are multiple cell-line precursors of Merkel cell carcinoma. (medscape.com)

Expression data from bone marrow CLP and B-cell progenitor subfractions. (refine.bio)
Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
Bone marrow-derived stem cells Hematopoietic stem cells Adipogenesis Fibrin gel culture, 3-dimensional cultures Original Paper Cells Tissues Organs 2008;187:186 198 DOI: 10.1159/000111804 Accepted after revision: August 29, 2007. (karger.com)
Mouse IL-7 was initially described as a pre B-cell grow factor expressed in bone marrow stromal cells. (biolegend.com)
The bone marrow microenvironment (BMME) is a complex network system primarily comprising stromal cells such as mesenchymal stem cells (MSCs), osteoprogenitor cells, vascular endothelial cells, monocytes, macrophages, and cytokines. (techscience.com)
The bone marrow stromal cell (BMSC) population, which makes up a large part of the hematopoietic microenvironment, encompasses a variety of adherent cell types. (techscience.com)
The rapid advancement in understanding the biology of hematopoietic stem cells (HSCs) and cancer stem cells (CSCs) in recent years has renewed interest in investigating the role of stem cells in benzene -induced malignancy and bone marrow depression. (cdc.gov)
The interplay between benzene and stem cells is complex involving the stem cell, progenitor, and HSC niche compartments of the bone marrow. (cdc.gov)
In this prospect, benzene metabolites formed through metabolism in the liver and bone marrow cause damage in hematopoietic cells via multiple mechanisms that, in addition to traditionally recognized chromosomal aberration and covalent binding, incorporate oxidative stress, alteration of gene expression, apoptosis, error-prone DNA repair, epigenetic regulation, and disruption of tumor surveillance. (cdc.gov)

Their findings indicate that postmortem tissue may be a potential source of multipotent stem cells, with a variety of uses and applications. (salk.edu)

Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)
Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. (scirp.org)
Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
Fehrer, C. and Lepperdinger, G. (2005) Mesenchymal Stem Cell Aging. (scirp.org)

Progenitors of the zebrafish laterality organ originate from the superficial epithelial enveloping layer by an apical constriction process of cell delamination. (elifesciences.org)
[ 5 , 6 ] Furthermore, experiments have demonstrated that epidermal progenitors in the touch domes are capable of producing Merkel cell lines and that epithelial progenitor populations in adults have the capacity to give rise to both neuroendocrine and squamous lineages. (medscape.com)
On the other hand, clear cell acanthoma is a rare, benign epithelial cutaneous tumor. (medscape.com)
Clear cell acanthoma usually stains positively for epithelial membrane antigen and negatively for carcinoembryonic antigen. (medscape.com)

Most clusters could be computationally assigned to a defined hematopoietic subpopulation, including progenitor cells (clusters 0 to 4) and a single transcriptionally defined HSC population (cluster 5). (confex.com)

This is a cheap, rapid, and highly efficient method of obtaining large quantities of viable CFU-E. The sequential formation of two-, four-, and eight-cell colonies from CFU-E cultured in vitro was studied. (silverchair.com)
02:47 and red blood cells in vitro. (yale.edu)
To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
Described here are some of the general requirements of in vitro test methods for skin sensitization, and progress that has been made in developing suitable approaches with particular emphasis on the utility of dendritic cell culture systems. (cdc.gov)

Finally, by using in-utero electroporation to delete RhoA in individual cells, we found out that the misplacements of neurons in the heterotopic cortex was not due to an inability of RhoA-neg. (uni-muenchen.de)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)

Mnx1 expression has been shown to be important for the development of dorsal Pdx1, hence acting as a necessary transcription factor for the specification of foregut endoderm into Pdx1 expressing pancreatic progenitors. (wikipedia.org)
It is important to note here that the developing dorsal and ventral buds are characterized as endoderm, and it isn't until the expression of Pdx1 (specification of endoderm to a stem cell multipotent state) that the endoderm to pancreatic progenitor transition occurs. (wikipedia.org)
In particular, we study the foregut progenitor cells in the ventral endoderm, which are the early building blocks of the liver, lungs and pancreas. (cincinnatichildrens.org)

This idea is based on previous experiences with the deletion of Cdc42 in the developing neocortex, which leads to a loss of apical progenitors. (uni-muenchen.de)
apical progenitors and Tbr2-pos. (uni-muenchen.de)
Investigating the cellular architecture of the developing cortex, we observed a loss of the radial orientation of radial glial cells, likely due to the disruption of the apical band of adherens junctions, which is the first effect observed after loss of the protein, and the consequent formation of rosette like structures in the brain parenchyma. (uni-muenchen.de)

These cells have been shown to have 28 genes regulating the cell cycle to be upregulated, showing that they are proliferative cells having the ability to replace and give rise to multiple cell populations in the pancreas. (wikipedia.org)
The pancreas is an organ containing both exocrine and endocrine cell populations. (biomedcentral.com)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

However, sustained engraftment of the expanded cells was not observed after transplantation, suggesting ineffective expansion of hematopoietic stem cells with long-term repopulating activity (LTR-HSCs). (confex.com)
At optimal coating densities of Delta1, the frequency of LTR-HSCs measured by limiting dilution analysis 16 weeks after transplantation into NSG mice was 4.9- and 4.2-fold higher in hypoxic cultures (1 in 1,586 CD34+ cells) compared with uncultured cells (1 in 7,706) and the normoxia group (1 in 5,090), respectively. (confex.com)
Conversely, we observed no difference in expression of the homing CXCR4 receptor between cells cultured under normoxic and hypoxic conditions, indicating that hypoxia increased the absolute numbers of LTR-HSCs but not their homing potential after transplantation. (confex.com)
To corroborate these findings molecularly, we performed transcriptomic analyses and found significant upregulation of a distinct HSC gene expression signature in cells cultured with Delta1 in hypoxia (Fig. A). Collectively, these data show that hypoxia supports a superior ex vivo expansion of human HSCs with LTR activity compared with normoxia at optimized densities of Delta1. (confex.com)
To clarify how hypoxia improved Notch-mediated expansion of LTR-HSCs, we performed scRNA-seq of CD34+ cells treated with Delta1 under normoxic or hypoxic conditions. (confex.com)
Hypoxia lessened the cellular stress response in both progenitors and HSCs, but the mitigation was more apparent in the HSC population (Fig. C, grey plots), and decreased apoptosis was observed only within the HSC-enriched cluster 5 (Fig. D). These findings are consistent with several reports indicating that HSCs are more vulnerable to strong ER stress than downstream progenitors due to their lower protein folding capacity. (confex.com)

Notch signaling has been shown to regulate the number of exocrine and endocrine cells in the pancreas, but not without the presence of Pdx1. (wikipedia.org)
Regulate the growth of hematopoietic stem cells and promote the survival of primitive hematopoietic progenitor cells. (biolegend.com)
They can regulate the neurotransmitters and the external chemical environment around nerve cells to influence how often nerve cells send impulses and thus regulate how active groups of nerve cells may be. (msdmanuals.com)
Numerous factors localize at telomeres to regulate their length, structure and function, to avert replicative senescence or genome instability and cell death. (cdc.gov)

Neurodegeneration and neurodegenerative diseases (including the shared mechanisms of nerve cell death that contribute to many diseases), Vascular Cognitive Impairment and Dementia (VCID), NINDS tissue/cell resources, basic invertebrate neuromuscular junction (NMJ). (nih.gov)
Molecular mechanisms of childhood liver cancer with a focus on hepatic stem/progenitor cells. (cincinnatichildrens.org)

Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)

This transcription factor has been shown to give rise to the multipotent stem cell lineages contributing to the endocrine, exocrine and ductal cells of the pancreas. (wikipedia.org)
During embryo development, naïve cell lineages undergo concurrent processes of fate specification and morphogenesis as critical steps towards the generation of differentiated tissues and organs. (elifesciences.org)
Hematopoietic stem cells (HSC) give rise to all types of blood lineages, including red blood cells (RBC). (lu.se)

2022. Cell Rep. 41:111677. (biolegend.com)
2022. Cell Res. (biolegend.com)

hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
Cells Tissues Organs (2021) 209 (4-6): 209-214. (karger.com)
Cells Tissues Organs (2019) 207 (1): 15-20. (karger.com)
Cells Tissues Organs (2008) 188 (1-2): 139-149. (karger.com)
Cells Tissues Organs (2008) 187 (3): 186-198. (karger.com)
Cells Tissues Organs (2005) 180 (4): 195-203. (karger.com)
Therapeutic potentials of mesenchymal stem cells (MSCs) depend largely on their ability to secrete cytokines or factors that modulate immune response, enhance cell survival, and induce neovascularization in the target tissues. (hindawi.com)
Basement membrane (BM) is a sheet-like extracellular matrix that underlies most tissues and acts as a barrier to invading cells. (duke.edu)
Mechanochemical Principles of Spatial and Temporal Patterns in Cells and Tissues. (mpi-cbg.de)
Patterns are ubiquitous in living systems and underlie the dynamic organization of cells, tissues, and embryos. (mpi-cbg.de)
During development, stem cells and resulting progenitor cells are responsible for generating all the tissues and cells of an organism. (wisc.edu)
The cells derived from these tissues constitute a model system for studying the brain cell biology of these disorders, according to Gage. (salk.edu)

Proangiogenic hematopoietic progenitor cell research requires multidisciplinary expertise in flow cytometry , hematology , and vascular biology . (bvsalud.org)
Previous studies demonstrated that MSCs could ameliorate the pathology associated with ischemic heart disease, ischemic stroke, and peripheral vascular disease by releasing several critical factors that increase cell survival, provide an appropriate microenvironment for repairing damaged tissue, and induce neovascularization [ 3 - 5 ]. (hindawi.com)
It is well established that endothelial cells (EC) play a crucial role in vascular physiology. (rug.nl)

refine.bio: a resource of uniformly processed publicly available gene expression datasets. (refine.bio)
The Rho family of small GTPases is known for its many cell biological functions such as regulation of the cytoskeleton, gene expression, cell migration, adhesion, cell polarity and the cell cycle. (uni-muenchen.de)

To determine whether Notch in fact does act in Ngn3-expressing cells in vivo will require further studies relying on conditional mutagenesis. (biomedcentral.com)
Notch activation in human CD34+ hematopoietic stem/progenitor cells (HSPCs) by treatment with Delta1 ligand has enabled clinically relevant ex vivo expansion of short-term HSPCs. (confex.com)
Minogue AL, Arur S*. In Situ Hybridization for Detecting Mature MicroRNAs In Vivo at Single-Cell Resolution. (mdanderson.org)
IL-7 also controls T cell-dendritic cell interactions that are essential for both T-cell homeostasis and activation in vivo . (biolegend.com)

A main focus of my lab research is to understand the epigenetic basis of cell fate specification and neurodevelopment. (wisc.edu)

These cells initially amplify and colonize the entire CNS and maintain the proliferating ability even during adult life 5 . (nature.com)
Human adult mobilized CD34+ cells were cultured for 21 days under normoxia (21% O 2 ) or hypoxia (2% O 2 ) in vessels coated with optimized concentrations of Delta1. (confex.com)
Previous attempts to recover progenitor cells from adult tissue had failed, but the Gage laboratory applied growth factors to extracted tissue that appear to have made a crucial difference. (salk.edu)
Testing in whole animals is the only way to know if adult tissue can be a source of stem or progenitor cells for transplant purposes to treat neurodegenerative disease," said Gage. (salk.edu)
Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)

The success of this journey is critical when the number of progenitors in a tissue or organ is limited. (elifesciences.org)
We used the CliniMACS Plus system located in our Cell and Tissue. (karger.com)
The easy accessibility of hematopoietic cells, which are not tightly embedded in tissue, has supported. (karger.com)
We studied the secretome profile of gestational tissue-derived MSCs and their effects on functions of endothelial progenitor cells (EPCs), another angiogenic cell type that plays an important role during the neovascularization. (hindawi.com)
Tissue from older individuals yielded fewer proliferating cells. (salk.edu)
This study employed a pool of cells from extracted tissue," said Gage. (salk.edu)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

It was reported that the receptor tyrosine kinase Flt3 is required for dendritic cell development. (biolegend.com)

They give rise to both the endocrine and exocrine cells. (wikipedia.org)
Lineage tracing studies have revealed that both endocrine and exocrine cells are derived from Pdx1 -expressing progenitors [ 1 - 3 ]. (biomedcentral.com)

Reyes-Castro R, Chen SY, Seemann J, Kundu ST, Gibbons D, Arur S . Phosphorylated nuclear DICER1 promotes open chromatin state and lineage plasticity of AT2 tumor cells in lung adenocarcinomas. (mdanderson.org)
2021). The antiandrogen enzalutamide downregulates TMPRSS2 and reduces cellular entry of SARS-CoV-2 in human lung cells . (essex.ac.uk)

Although several subtypes of T-cell lymphoblastic leukemia/lymphoma exist, early T-cell precursor lymphoblastic leukemia (ETP-ALL) is the only subtype recognized as an entity in the revised 2016 WHO tumor classification. (medscape.com)
Non-ETP subtypes of T-cell lymphoblastic leukemia/lymphoma, in contrast, are associated with activating NOTCH1 mutations in over half of all patients and an additional 10% to 15% of cases have FBXW7 mutations, which also result in increased NOTCH signaling. (medscape.com)

2020. Cell. (biolegend.com)

Molecular biology of cancer, tumor microenvironment and metastasis. (cincinnatichildrens.org)

Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)

Dr. Jason Doles (pronouns He/Him/His) is an Associate Professor in the Department of Biochemistry and Molecular Biology at Mayo Clinic. (jove.com)
Methods in Molecular Biology , 549 , 217 - 230. (bris.ac.uk)
Hematopoiesis, molecular biology, and molecular oncology including mouse modeling of hematopoiesis, myelopoiesis and leukemia. (cincinnatichildrens.org)

The development of beta cells precedes that of the development of other endocrine cells in the islets. (wikipedia.org)
In a first-of-its-kind look at human kidney development, researchers at The Saban Research Institute of Children's Hospital Los Angeles have isolated human nephron progenitor (NP) cells. (biologynews.net)
With sensorineural hearing loss (SNHL), the damaged hair cells of the organ of Corti within the cochlea interfere with typical hearing and, as a result, cause impaired language development. (intechopen.com)
Several studies have demonstrated that Notch signaling is involved in the development of endocrine cells in the pancreas. (biomedcentral.com)
During development, hematopoiesis and neovascularization are closely linked to each other via a common bipotent stem cell called the hemangioblast that gives rise to both hematopoietic cells and endothelial cells . (bvsalud.org)
IL-7 is essential for normal murine B cell development, and plays a key role in regulating the homeostasis and function of the T-cells. (biolegend.com)

2021. Stem Cell Reports. (biolegend.com)
2021. Cell Metab. (biolegend.com)

Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)

Some of the impulses stimulate the next nerve cell, whereas others inhibit it. (msdmanuals.com)

Finally, we provide protocols for analyzing these cells through flow cytometry and immunocytochemistry. (lu.se)

Isolation and characterization of the erythroid progenitor cell: CFU-E. (silverchair.com)

Anatomy35

Organisms7

Diseases2

Chemicals and Drugs30

Analytical, Diagnostic and Therapeutic Techniques and Equipment27

Phenomena and Processes31

Disciplines and Occupations9

Technology, Industry, Agriculture2

Humanities1

Information Science2

Health Care1

Differentiation35

Neural17

Embryonic12

Differentiate9

Pathways6

Pancreatic progenitor cells3

Myeloid4

Proliferation12

Hematopoietic progenitors3

Subset1

Oligodendrocyte progenitor2

Genetic6

Regeneration8

Stem cell biology3

Developmental3

Lymphoid1

Basal4

Fetal3

Precursors2

Bone11

Multipotent stem1

Mesenchymal Stem4

Epithelial4

Subpopulation1

Vitro5

Neurons3

Endoderm3

Apical3

Populations4

HSCs6

Regulate4

Mechanisms2

Phenotypes1

Lineages3

20222

Tissues12

Vascular3

Gene expression2

Vivo4

Specification1

Adult5

Tissue8

Kinase1

Endocrine and exocrine cells2

Lung2

Subtypes2

20201

Microenvironment1

Transition1

Molecular3

Development6

20212

Umbilical1

Stimulate1

Flow cytometry1

Characterization1