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  • Dendritic Cells
  • Dendritic cells (DCs) serve a crucial function in the immune system as the major antigen presenting cells with the unique ability to activate naive T cells, 1 a capacity that has made them the major target of therapeutic manipulation in vaccination and cancer immunotherapy protocols. (bloodjournal.org)
  • endothelial
  • 1 - 5 Similarly, after cutaneous injury, a heterogeneous population of BMDCs are recruited to the site of injury and contribute directly to the repair process by differentiating into skin cell types, such as fibroblasts, keratinocytes, and endothelial cells. (bloodjournal.org)
  • HOXA7 and HOXB3 are up-regulated during the differentiation of human BMD mesenchymal stem cells into endothelial cells, while HOXA3 and HOXB13 are down-regulated during differentiation. (bloodjournal.org)
  • We have previously shown that sustained expression of HOXA3 in vivo can modulate BMD cell behavior in the wound microenvironment by changing the balance of endothelial progenitor and inflammatory cell recruitment/retention. (bloodjournal.org)
  • findings
  • These findings demonstrate that manipulation of adult stem/progenitor cells ex vivo could be used as a potential therapy in patients with impaired wound healing. (bloodjournal.org)
  • These findings fill a fundamental gap in the understanding of dynamic regulation of arginine methylation level in cells and also provide therapeutic potential of the JMJDB1 gene for associated disorders. (cityofhope.org)
  • These findings support an instructive but argue against a lineage-restrictive role of C/EBPα in multipotent hematopoietic and thymic progenitors. (bloodjournal.org)
  • These findings suggest that MM cells have a lower threshold for PI-induced UPR induction and ER stress-induced apoptosis because they constitutively express ER stress survival factors to function as secretory cells. (bloodjournal.org)
  • The findings suggest that diabetic patients could be treated with beta 2 blockers to reduce the number of inflammatory myeloid cells that cause plaque to rupture," said Dr. Dutta, the senior author of the study. (pitt.edu)
  • endogenous
  • Transplantation of Human Menstrual Blood Progenitor Cells Improves Hyperglycemia by Promoting Endogenous Progenitor Differentiation in Type 1 Diabetic Mice. (cyagen.com)
  • According to the results, we concluded that MBPCs stimulated β-cell regeneration through promoting differentiation of endogenous progenitor cells. (cyagen.com)
  • Using the minimal CRISPR interference (CRISPRi) system, this study showed that coexpression of the catalytically inactive Cas9 (dCas9) and guide RNAs targeting the endogenous roX locus in the Drosophila cells results in a robust and specific knockdown of roX1 and roX2 RNAs, thus eliminating the need for recruiting chromatin modifying proteins for effective gene silencing. (sdbonline.org)
  • activates
  • 2-6 Cyclin E binds to and activates cdk2 protein kinase, and in proliferating cells the assembly of catalytically active cyclin E-cdk2 complexes is directly related to the abundance of cyclin E protein. (bloodjournal.org)
  • Erythropoietin activates target erythroid colony-forming cells by binding and orienting two cell surface erythropoietin receptors. (kenyon.edu)
  • Each molecule of EPO activates cells by binding two identical cell-surface receptors. (kenyon.edu)
  • EPO activates target cells by binding and orienting two identical cell-surface receptors, EPObp1 and EPObp2, triggering an intracellular phosphorylation cascade. (kenyon.edu)
  • Mice
  • Histological and immunohistochemistry analyses indicated that T1DM mice with MBPC transplantation recovered islet structures and increased the β-cell number. (cyagen.com)
  • Consequently, these JMJD1B-deficient mice developed blood-cell disorders including an increase in white blood cells (lymphocytosis) and a decrease in red blood cells (mild anemia), and other phenotypes of myelodysplastic syndrome. (cityofhope.org)
  • In particular, the mice displayed a distinct blood cell lineage distribution, with an increase in the neutrophil cell population. (cityofhope.org)
  • Despite predicted roles for this factor during early development, GATA-1-deficient cells in chimeric mice and embryonic stem cell cultures mature to a late proerythroblast stage and express at least certain genes that normally are thought to be regulated by GATA-1 (including erythroid Kruppel-like factor [EKLF] and the erythropoietin [Epo] receptor). (sdbonline.org)
  • Studying patients and mice with diabetes, Dr. Dutta and his team found that when the fight-or-flight response is triggered, it also triggers the over-production of fighting white blood cells, or leukocytes. (pitt.edu)
  • Transplantation
  • Herein, we studied the therapeutic effect of MBPCs and the mechanism of β-cell regeneration after MBPC transplantation in the T1DM model. (cyagen.com)
  • We analyzed a series of genes associated with the embryonic mode of β-cell development by real-time polymerase chain reaction and the results showed that the levels of those gene expressions all increased after cell transplantation. (cyagen.com)
  • Expansion of human stem cells in ex vivo culture will likely have important applications in transplantation, stem cell marking, and gene therapy (2). (scielo.br)
  • Stem cell transplantation might be an option. (diseaseinfosearch.org)
  • monocytes
  • Differentiation of normal human monocytes to DCs led to a rapid increase of PU.1 to high levels that preceded phenotypic changes, but no MafB expression, whereas monocyte-derived macrophages expressed MafB and only moderate levels of PU.1. (bloodjournal.org)
  • DCs inducing levels of PU.1 inhibited MafB expression in monocytes, which appeared to be required for DC specification, since constitutive MafB expression inhibited DC differentiation. (bloodjournal.org)
  • 2 When exiting the blood stream, monocytes can give rise to inflammatory macrophages or to antigen presenting DCs. (bloodjournal.org)
  • gene
  • Single-cell gene expression analysis reveals CML stem cell heterogeneity and changes imposed by TKI therapy. (bloodjournal.org)
  • Here we have combined high-throughput immunophenotypic screens with large-scale single-cell gene expression analysis to define the heterogeneity within the LSC population in chronic phase chronic myeloid leukemia (CML) patients at diagnosis and following conventional tyrosine kinase inhibitor (TKI) treatment. (bloodjournal.org)
  • Arginine methylation of histone (H4R3me2s), an epigenetic modification, is carried out by the PRMT5 gene and has been implicated in various cellular processes including blood cancers . (cityofhope.org)
  • The research work, "JMJD1B Demethylates H4R3me2s and H3K9me2 to Facilitate Gene Expression for Development of Hematopoietic Stem and Progenitor Cells," carried out by C, Shafat Ali and Xiaotao Duan (co-first author) at the Beckman Research Institute of City of Hope, was published in the April 10, 2018 issue of the journal Cell Reports . (cityofhope.org)
  • This study discovered that active arginine demethylation process exists in eukaryotes and that JMJD1B demethylates both methylated arginine/lysine residue of histones for epigenetic programming of gene expression during blood-cell development and differentiation. (cityofhope.org)
  • Thus, understanding the dynamic regulation of arginine-methylation level in the cells is cirtical to developing additional therapeutic targets to improve cancer cell response to current agents that work by regulating arginine-methylation lavel and gene expression. (cityofhope.org)
  • Hypoxia causes an increase in erythropoietin gene transcription, enhancing red blood cell production. (kenyon.edu)
  • macrophages
  • To their surprise, they found that cells that expressed high levels of the transcription factor became exclusively macrophages, while cells expressing low levels of PU.1 developed into B cells. (uchospitals.edu)
  • Macrophages tightly control the production and clearance of red blood cells (RBC). (frontiersin.org)
  • In addition, we will discuss the interactions between these two cell types during transfer of immune complexes and pathogens from RBC to macrophages. (frontiersin.org)
  • immune
  • BMD Gr-1 + CD11b + cells function as immune suppressor cells and contribute significantly to tumor-induced neovascularization. (bloodjournal.org)
  • Gr-1 + CD11b + cells are a subset of BMD myeloid cells that are recruited to tumors in vivo and can function as immune suppressor cells with proangiogenic capacity in the tumor microenvironment. (bloodjournal.org)
  • MicroRNAs: new regulators of immune cell development and function. (springer.com)
  • Interestingly, the minor CD16 − pNK cell subset appears to contain the main cytokine-producing pNK cells and may be important in directing immune responses ( 4 ). (pnas.org)
  • In earlier studies, Singh and his colleagues established that PU.1 is required for the development of the white blood cells, which are important players in the immune system. (uchospitals.edu)
  • Singh and DeKoter are now studying how other transcription factors interact with PU.1 to control the development of immune cells. (uchospitals.edu)
  • Once we have a deeper understanding of the regulatory circuitry, it may be possible to exploit this knowledge to efficiently generate specific immune cells from progenitors for therapeutic purposes. (uchospitals.edu)
  • Hematopoietic dysregulation can result in an excess or a lack of certain blood cell types, leading to immune system disorders, cancer, various anemias, or coagulopathy. (qiagen.com)
  • Megakaryocytic
  • Megakaryocytic differentiation is accompanied by changes in cell morphology, notably an increase in size and the appearance of demarcation membranes, and by the sequential expression of a number of genes coding for specific cell surface markers, cytokines, and cytokine receptors. (bloodjournal.org)
  • tissues
  • How cell-intrinsic factors control the differentiation of BMD stem/progenitor cells recruited to peripheral tissues in response to injury is poorly understood, although Hox transcription factors have been shown to extensively modulate BMDCs. (bloodjournal.org)
  • Similar changes may occur upon NK cell entry into the decidua or other tissues expressing substantial TGFβ. (pnas.org)
  • Labeled cells from mouse splenocytes or other tissues are targeted for removal by Streptavidin RapidSpheres™ and separated without the use of columns using an EasySep™ magnet. (stemcell.com)
  • developmental
  • The idea that differing concentrations of a transcription factor can control the development of different cell types from progenitors has been a commonly held view among developmental biologists,' said Harinder Singh, associate professor of molecular genetics and cell biology, Howard Hughes Medical Investigator, and senior author of the paper. (uchospitals.edu)
  • Now, researchers led by Singh have shown that different concentrations of a single transcription factor called PU.1 can cause blood cell progenitors to go down different developmental pathways. (uchospitals.edu)
  • subset
  • Conversely, they express higher levels of CD94/NKG2A than the CD16 + pNK cell subset ( 4 ). (pnas.org)