• CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. (wikipedia.org)
  • Stem cells are not specialized and the process of their specialization is called differentiation. (benthamscience.com)
  • The colony-forming unit (CFU) assay is a clonal, in vitro culture assay that measures the growth and frequency of functionally viable HSPCs by assessing the proliferation and differentiation of individual progenitor cells, resulting in the formation of discrete colonies in a semi-solid methylcellulose medium (such as MethoCult™ ) when supplemented with appropriate cytokines. (stemcell.com)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • iPSC modeling of severe aplastic anemia reveals impaired differentiation and telomere shortening in blood progenitors. (duke.edu)
  • During development, hematopoietic stem cells (HSCs) undergo stepwise differentiation changing their phenotype, functional properties, and locations. (ox.ac.uk)
  • Dorsomorphin dihydrochloride also inhibits bone morphogenetic protein (BMP) type I receptors (ALK2, ALK3 and ALK6), promotes cardiomyogenesis in mouse embryonic stem cells (ESCs) in vitro and promotes neural differentiation of hPSCs as part of a chemical cocktail. (tocris.com)
  • SOX2 is expressed in multipotent neuronal stem cells, and may aid to identify cells that are capable of self-renewal and multipotent differentiation (1-3). (dbs-india.com)
  • EE selectively increased cell differentiation into MSN phenotype and promoted host-to-graft connectivity. (lu.se)
  • DNMT3A is a critical regulator of hematopoietic stem cell (HSC) differentiation and somatic DNMT3A mutations are frequent in hematologic malignancies and clonal hematopoiesis. (haematologica.org)
  • Here, I review the historical and current ideas of cell identification and identification transitions, and discuss how new tools and sights may instruct the near future knowledge of differentiation and vegetable regeneration. (cancercurehere.com)
  • These fresh views of cell differentiation and identity are undergoing rapid development and so are more likely to change. (cancercurehere.com)
  • Nevertheless, the idea of a rigid hierarchy of cell areas leading from an immature to a differentiated cell has been eliminated and changed by a far more liquid and flexible look at of cell identification transitions and differentiation. (cancercurehere.com)
  • This cells can go through differentiation to create both shoots and origins, and therefore it was recommended that callus cells are inside a pluripotent condition (Ikeuchi et al. (cancercurehere.com)
  • In addition, cluster of differentiation 24 (CD24)-negative NP responder clones formed spheroid structures in various culture systems, suggesting the preservation of a more immature phenotype compared to CD24-positive nonresponder clones. (biomedcentral.com)
  • Differential cell surface marker expression and divergent responses to differentiation conditions suggest that the NP subtypes may correspond to distinct maturation stages and represent distinct NP cell subpopulations. (biomedcentral.com)
  • The presence of 'stem cell-like' cells has previously been postulated based on sphere-forming capacity and differentiation in vitro 1 and evidence for the presence of label-retaining cells in the distal fallopian tube 2 . (nature.com)
  • Our data reveal a significant overlap between Notch-dependent genes in the fallopian epithelium and the defined 'stem cell signature' of the mouse intestine, suggesting the existence of a conserved pathway that regulates tissue renewal and directly controls cell fate specification and differentiation in the organoid by inhibiting cilliogenesis. (nature.com)
  • The intricate and dynamic balance between self-renewal, proliferation and differentiation of stem/progenitor cells of the salivary gland (SG) must be tightly regulated to ensure proper morphogenesis, homeostasis and regeneration. (grantome.com)
  • However, our current knowledge of the molecular mechanisms by which ?Np63 directs gene expression programs necessary for the commitment, maintenance and differentiation of the stem/progenitor cell population in the SG is lacking. (grantome.com)
  • Such systematic studies are much needed as they will identify for the first time, the functional role of p63 in SG development and in orchestrating stem/progenitor cell differentiation programs. (grantome.com)
  • During animal development, epithelial stem and progenitor cells of the salivary gland undergo a number of dynamic processes of maturation and differentiation, all of which require the coordinated action of functionally important transcription factors such as p63. (grantome.com)
  • Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
  • Optimal differentiation was verified by qPCR and immunofluorescent (IF) staining of cell-type specific markers. (cdc.gov)
  • Results: qPCR analysis of the differentiation time-course shows a steady increase in markers of terminally differentiated cells, such as club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1). (cdc.gov)
  • The basal cell (stem/progenitor cell) marker (KRT5) shows an initial increase in expression, but did not vary much during differentiation. (cdc.gov)
  • Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)
  • Abnormal proliferation, clonal expansion, aberrant differentiation, and diminished apoptosis (programmed cell death) lead to replacement of normal blood elements with malignant cells. (msdmanuals.com)
  • Britain s House of Lords final approval of therapeutic human cloning and embryonic stem cells research has intensified the battle for ascendancy between adult and embryonic stem cells. (i-sis.org.uk)
  • This latest anti-publicity on adult stem cells comes on the heels of a paper announcing success in embryonic stem (ES) cell transplant in a Parkinson rat model published in the house journal of the United States National Academy of Sciences [5]. (i-sis.org.uk)
  • In the current issue of the same journal [6], researchers compared the frequency and type of mutation induced in embryonic stem cells and embryonic somatic cells. (i-sis.org.uk)
  • They found that the spontaneous mutation frequency in ES cells is 100-fold lower than that in mouse embryonic fibroblasts (a somatic cell line), which is similar to adult cells in vivo . (i-sis.org.uk)
  • Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
  • The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
  • Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
  • Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
  • He predicted that cancers occur upon activation of dormant embryonic-like cancerous cells present in mature tissues but not spontaneously [ 8 ]. (biomedcentral.com)
  • There are three main stages of mammary gland development both in rodents and humans: embryonic, pubertal and adult. (biologists.com)
  • The hematopoietic system develops in waves: in all vertebrate species, early transitory embryonic waves are gradually replaced by a permanent adult hierarchy which persists throughout the animal's lifespan. (ox.ac.uk)
  • Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
  • SOX2, a persistent marker for multipotential neural stem cells derived from embryonic stem cells, the embryo or the adult. (dbs-india.com)
  • Cell replacement using human embryonic stem cells (hESCs) derivatives may offer new therapeutic opportunities to replace degenerated neurons and repair damaged circuits. (lu.se)
  • When cultivated on cell culture dishes coated with 6C3-rich CS/DS, corneal stromal cells (keratocytes) that had been isolated from embryonic chicken corneas formed circular colonies, which took several days to reach confluency. (cardiff.ac.uk)
  • Sandström J., Eggermann E., Charvet I., Roux A., Toni N ., Greggio C., Broyer A., Monnet-Tschudi F., Stoppini L. "Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing. (chuv.ch)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
  • Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)
  • To study how radiation affects neurogenesis, we combine phenotyping of subpopulations of hippocampal neural stem and progenitor cells with double- and triple S-phase labeling paradigms. (scite.ai)
  • Consequently, neural stem and progenitor cells have usually been studied retrospectively, based on their ability to form colonies in laboratory cell cultures. (elifesciences.org)
  • Using ECM receptors as our guide, we recreated a defined microenvironment in vitro that mimics olfactory basal lamina and, when supplemented with epidermal growth factor, transforming growth factor α, and leukemia inhibitory factor, allows us to preferentially expand multiple clonal adherent colony phenotypes from individual ICAM-1 + and ICAM-1+/β 1 integrin+-selected HBCs. (syr.edu)
  • HBCs thus possess a conserved adhesion receptor expression profile similar to non-neural stem cells, preferential self-replication in an in vitro environment mimicking their in vivo niche, and contain subpopulations of cells that can produce multiple differentiated neuronal and glial progeny from within and beyond the olfactory system in vitro. (syr.edu)
  • Lgr5 resides in Wnt receptor complexes and mediates signaling of the Wnt-agonistic R-spondins (31), explaining the unique dependence of Lgr5 stem cells on secreted R-spondins in vivo and in vitro. (hubrecht.eu)
  • Other uses of the CFU assay include studying the effects of stimulatory and inhibitory growth factors, screening novel compounds to predict potential toxicity to the hematopoietic system, and testing the effects of various in vitro manipulations (e.g. cell processing, cryopreservation, gene transduction, and transmission) on cellular products used in hematopoietic cell transplantation. (stemcell.com)
  • Differently from MAPCs our PDPCs were unable to differentiate into endothelial cells after their in vitro VEGF treatment. (univpm.it)
  • Troy+ cells have a 40-fold higher capacity than Troy- cells to form organoids, which is considered a stem cell property in vitro. (knaw.nl)
  • In vitro neurosphere assays revealed striking increases in the number of neurosphere forming cells from p107 −/− brains that exhibited enhanced capacity for self-renewal. (rupress.org)
  • We show that single epithelial stem cells in vitro can give rise to differentiated organoids containing ciliated and secretory cells. (nature.com)
  • Here we demonstrate the existence of adult stem cells in the human fallopian tube epithelium, which gives rise to a monolayer of differentiated epithelial cells in a complex 3D organoid in vitro . (nature.com)
  • At the crypt base, in the vicinity of basal lamina, stem cells are surrounded by mesenchymal cells that are of myofibroblast lineage. (biomedcentral.com)
  • In line with these studies, we observe a signi cant decrease in doublecortin, a marker for neuronal precursor cells/neurogenesis, in the subgranular zone in the dentate gyrus of the hippocampus, a major site of neurogenesis in the mouse brain, following irradiation, which remains reduced following monocyte in ltration ( Fig. (scite.ai)
  • Besides, opening up new avenues for promoting neuronal fitness and brain health , this research may also establish an important experimental paradigm for interrogating cell competition during neurodevelopmental processes and in neurodegenerative diseases. (phys.org)
  • The identification of endogenous cell competition among NPCs provides a fundamental basis for comprehending the developmental origin of neuronal vulnerability as well as improving brain health. (phys.org)
  • In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
  • Generation of neuronal progenitor cells in response to tumors in the human brain. (mbfbioscience.com)
  • Toni N ., Song H., Ming GL "Tangential migration of neuronal precursors of glutamatergic neurons in the adult mammalian brain", PNAS 2015 July 13. (chuv.ch)
  • POT1 mutation carriers had a range of benign and malignant neoplasms involving epithelial, mesenchymal, and neuronal tissues in addition to B- and T-cell lymphoma and myeloid cancers. (cdc.gov)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
  • Two reports appeared as advance online publications in the top British journal Nature , accompanied by a news report that begins, "The hyped ability of adult stem cells to sprout replacement tissue types is being called into question. (i-sis.org.uk)
  • The Organoid group, previously Clevers group, studies the molecular mechanisms of tissue development and cancer of various organs using organoids made from adult Lgr5 stem cells. (hubrecht.eu)
  • These cells represent the epithelial stem cells of the small intestine and colon (23), the hair follicle (24), the stomach (28) and many other tissue stem cell types. (hubrecht.eu)
  • Background Microglia, the primary resident myeloid cells of brain, play critical roles in immune defense by maintaining tissue homeostasis and responding to injury or disease. (scite.ai)
  • Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
  • Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
  • Studies have shown that cell-to-cell mitochondrial transfer plays an essential role in regulating cardiovascular system development and maintaining normal tissue homeostasis under physiological conditions. (frontiersin.org)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • Similarly, in multicellular organisms, competitive interactions also occur between cells, creating a cell selection mechanism that eliminates less fit cells within the local tissue environment. (phys.org)
  • Human Periosteum-Derived Stem Cells for Tissue Engineering Applications: The Role of VEGF. (univpm.it)
  • Correct selection of the MSCs source is crucial in order to obtain a more efficient treatment and, in this respect Periosteum-Derived Cells (PDPCs) may represent an interesting alternative to bone marrow MSCs for osteochondral tissue regeneration. (univpm.it)
  • Since the latter are able to differentiate into endothelial cells, we tested the possible PDPCs commitment toward an endothelial phenotype in view of bone tissue engineering approaches that takes into account not only bone formation but also vascularization. (univpm.it)
  • Under normal circumstances, turnover of epithelial cell lineages occurs constantly in every 2-7 days but this process become faster during tissue repair and wound healing [ 5 ]. (biomedcentral.com)
  • This article focuses on tissue origin and mechanisms underpinning the development of the adult hematopoietic system in light of analysis of different vertebrate models. (ox.ac.uk)
  • 2017, Z. around the emergence of patterns and tissue organization, and information regarding the events occurring at the level of individual cells is only now beginning to emerge. (cancercurehere.com)
  • Nevertheless, research in tissue tradition have shown that whenever induced by exterior hormone software, callus originates particularly from specific pericycle-like cells discovered throughout the vegetable (Atta et al. (cancercurehere.com)
  • Here we report the generation of clonal NP cell lines from nondegenerate human IVD tissue and present a detailed characterization of NP cellular subpopulations. (biomedcentral.com)
  • The initial stages of DDD correlate with reduced cellularity, aberrant cell function, loss of proteoglycans and concomitant tissue dehydration [ 6 ]. (biomedcentral.com)
  • Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
  • Prior experience in performing in situ hybridisation on Nematostella adult tissue is also essential. (scholarshipdb.net)
  • Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
  • These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair. (scirp.org)
  • First, we will use conditional knockout mouse models to examine the role of ?Np63 in SG morphogenesis and in adult tissue maintenance and repair (Aim1). (grantome.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • In individuals with this condition, pancreatic acinar cells do not develop in utero and are replaced by fatty tissue. (medscape.com)
  • The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)
  • Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
  • These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • Neither report cited a paper published last year in the journal Blood [4], where a group from the Stem Cell Institute, Department of Medicine, and Cancer Center, University of Minnesota Medical School, Minneapolis, reported the most comprehensive experiments proving that a single adult stem cell can differentiate into all cell types in culture. (i-sis.org.uk)
  • The cells could be made to differentiate into bone forming cells, cartilage forming cells, fat cells, skeletal muscle cells and endothelial cells. (i-sis.org.uk)
  • Smad4 deletion in stem, but not progenitor cells, as well as Noggin infusion lead to an increased number of Olig2-expressing progeny that migrate to the corpus callosum and differentiate into oligodendrocytes. (cipsm.de)
  • Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
  • Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)
  • The mesoderm gives rise to hematovascular progenitors which through cellular intermediates go on to differentiate into blood. (ox.ac.uk)
  • Human induced pluripotent stem (iPS) cells can differentiate into hepatocyte lineages, although the phenotype of the differentiated cells is immature compared to adult hepatocytes. (ca.gov)
  • Proteins are the machinery of the cell, responsible for executing the functions essential for cells to operate, survive, differentiate and divide. (lu.se)
  • Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • On the same day, the cells in the apical chamber were exposed to air (airlift) to create the ALI and allowed to differentiate for 28 days. (cdc.gov)
  • A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
  • We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
  • CD34+ is often used clinically to quantify the number of haemopoietic stem cells for use in haemopoietic stem cell transplantation. (wikipedia.org)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • Some in vivo transplantation studies have reported robust (35-50%) levels of transdifferentiation, which makes it unlikely that the results are due to cell fusion events. (i-sis.org.uk)
  • These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (32, 47, 53). (hubrecht.eu)
  • Transplantation experiments further verified the cell-autonomous nature of this phenotype. (cipsm.de)
  • We also discussed the advantage and challenges of mitochondrial transfer strategies, including cell-based mitochondrial transplantation, extracellular vesicle-based mitochondrial transplantation, and naked mitochondrial transplantation, for the treatment of cardiovascular disorders. (frontiersin.org)
  • Between February 1986 and March 1990, 56 patients with relapsed Hodgkin's disease treated with high-dose cyclophosphamide, carmustine, and etoposide (CBV) received an autologous peripheral stem cell transplantation (PSCT) rather than an autologous bone marrow transplantation (ABMT) because each patient had a marrow abnormality, either hypocellularity or tumor involvement. (shengsci.com)
  • Preemptive immunotherapy in childhood acute myeloid leukemia for patients showing evidence of mixed chimerism after allogeneic stem cell transplantation. (shengsci.com)
  • Previous studies have shown that children with acute myeloid leukemia (AML) who developed mixed chimerism (MC) were at high risk for relapse after allogeneic stem-cell transplantation (allo-SCT). (shengsci.com)
  • These findings may help to generate mature liver cells for drug toxicity studies and ultimately for liver transplantation. (ca.gov)
  • Results: Human striatal progenitors survived up to 6 months after transplantation and showed morphological and neurochemical features typical of human MSNs. (lu.se)
  • Treatment typically includes combination chemotherapy to achieve remission, intrathecal and systemic chemotherapy and/or corticosteroids for CNS prophylaxis, and sometimes cerebral irradiation for intracerebral leukemic infiltration, consolidation chemotherapy with or without stem cell transplantation, and maintenance chemotherapy for up to 3 years to avoid relapse. (msdmanuals.com)
  • The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • They may instead be fusing with existing cells, creating genetically mixed-up tissues with unknown health effects" [1]. (i-sis.org.uk)
  • In mammals, physiological Wnt signaling is intimately involved with the biology of adult stem cells and self-renewing tissues (18,19). (hubrecht.eu)
  • Mesenchymal stem cells (MSCs) are promising tools for studying the mechanisms of development and for the regeneration of injured tissues. (univpm.it)
  • It's possible that one cell types, just like the pericycle, are primed and may quickly acquire pluripotency currently, while cells from additional tissues have to go through a competence acquisition stage before their pluripotent potential turns into apparent. (cancercurehere.com)
  • These findings support the view that CS/DS promotes the retention of a stem cell phenotype in corneal cells, much as it has been proposed to do in other connective tissues. (cardiff.ac.uk)
  • Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
  • Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
  • MSCs are adult progenitor cells that function as the body's early responders, poised to take action to help repair damaged tissues, jumping from their niches in the bone, for example, into the blood, migrating to areas of inflammation, and orchestrating the body's reactions during wound healing. (newswise.com)
  • Long-term clonal culturing of organoids from Lgr5 stem cells. (hubrecht.eu)
  • To investigate cell competition between NPCs, the researchers first developed a novel strategy for genetic mosaic induction and clonal tracking based on a dual-color labeling system. (phys.org)
  • Specifically, Axin2-mutant stem cells were eliminated and even engulfed, whereas p53-deficient stem cells underwent clonal expansion in the mosaic environment and became winner cells. (phys.org)
  • To determine whether cell competition naturally occurs in the developing brain , the researchers collected more than 1,000 mouse brains with clonal labeling of NPCs and studied their fate using short- and long-term lineage tracing. (phys.org)
  • Using single-cell qRT-PCR and clonal analyses, here we define four subpopulations of cardiac progenitor/stem cells in adult mouse myocardium all sharing stem cell antigen-1 (Sca1), based on side population (SP) phenotype, PECAM-1 (CD31) and platelet-derived growth factor receptor-α (PDGFRα) expression. (ox.ac.uk)
  • Clonal progeny of single Sca1(+) SP cells show cardiomyocyte, endothelial and smooth muscle lineage potential after cardiac grafting, augmenting cardiac function although durable engraftment is rare. (ox.ac.uk)
  • According to unitarian theory, proposed in 70s, all four cell lineages within the gastrointestinal epithelium are clonal populations derived from a slowly cycling clonogenic stem cells (SC) [ 6 ]. (biomedcentral.com)
  • In this study, we aimed to investigate whether phenotypically distinct clonal cell lines representing different subpopulations in the human NP could be generated using immortalization strategies. (biomedcentral.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • Three main epithelial cell lineages comprise a crypt: the columnar cells or colonocytes, the mucin-secreting cells or goblet cells, and the enteroendocrine cells. (biomedcentral.com)
  • Recent studies have provided new insights into the origin, specification and fate of mammary stem and progenitor cells and into how the differentiated lineages that comprise the functional mammary gland are determined. (biologists.com)
  • A surprising recent discovery has been that mammary epithelial cells commit to differentiated lineages using the same signalling pathways that regulate lineage determination in T helper cells. (biologists.com)
  • Similarly, destruction of neonatally abundant pluripotent stem cells would likely have a more pervasive outcome than destruction of The value of incorporating immunologic appeared more severe and/or persistent when single lineages or differentiated cells that pre- data for the toxicologic assessment of drugs, the exposure occurred perinatally when com- dominate in adults. (cdc.gov)
  • The most highly mitotic colony-forming HBCs demonstrate multipotency, spontaneously generating more ICAM-positive presumptive HBCs, a combination of olfactory neuroglial progenitors, and neurons of olfactory and potentially nonolfactory phenotypes. (syr.edu)
  • Recent evidence from our laboratory has determined that lifelong proliferation of newborn neurons in the adult hippocampus, a feature of adult hippocampal plasticity that has been implicated in some aspects of learning and memory, is modulated by early choline availability. (duke.edu)
  • Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
  • Their findings showed that this manipulation caused a significant enlargement of brain size and a remarkable rise in the number of neurons, indicating the survival of otherwise unfit stem cells during development. (phys.org)
  • Conclusions: The data support the long-term therapeutic potential of ESC-derived human striatal progenitor grafts for the replacement of degenerated striatal neurons in HD and suggest that EE can effectively accelerate the maturation and promote the integration of human striatal cells. (lu.se)
  • Secondly, we have developed an all-in-one vector-based strategy to convert adult human dermal fibroblasts directly from Huntington's disease (HD) patients and control individuals into induced neurons (iNs). (lu.se)
  • However, microglial activation and dysfunction has been implicated in a number of central nervous system (CNS) disorders, thus developing tools to manipulate and replace these myeloid cells in CNS is of therapeutic interest. (scite.ai)
  • Methods Using whole body irradiation, bone marrow transplant, and colony-stimulating factor 1 receptor inhibition, we achieve long-term and brain-wide (~ 80%) engraftment and colonization of peripheral bone marrow-derived myeloid cells (i.e. monocytes) in the brain parenchyma and evaluated the long-term effects of their colonization in the CNS. (scite.ai)
  • Although our results show that microglial replacement with peripheral derived myeloid cells is feasible and that irradiation-induced changes can be reversed by the replacement of microglia with monocytes in the hippocampus, we also observe that brain-wide engraftment of peripheral myeloid cells (relying on irradiation) can result in cognitive and synaptic deficits. (scite.ai)
  • Conclusions These findings provide insight into better understanding the role and complexity of myeloid cells in the brain, including their regulation of other CNS cells and functional outcomes. (scite.ai)
  • 2) the impaired ability of SAA-iPSC-derived hematopoietic progenitors to give rise to erythroid and myeloid cells. (duke.edu)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • Cardiac progenitor/stem cells in adult hearts represent an attractive therapeutic target for heart regeneration, though (inter)-relationships among reported cells remain obscure. (ox.ac.uk)
  • The regeneration is maintained by adult stem cells. (biomedcentral.com)
  • Our data show that Troy marks a renal stem/progenitor cell population in the developing kidney that in adult kidney contributes to homeostasis, predominantly of the collecting duct, and regeneration. (knaw.nl)
  • 1958). This means that that as the pericycle, using its putative specific properties, may be the primary contributor to cells culture-based regeneration, pluripotency could be wide-spread amongst vegetable cells. (cancercurehere.com)
  • Detailed understanding of the ontogenetic, cellular and molecular characteristics of functional intervertebral disc (IVD) cell populations is pivotal to the successful development of cell replacement therapies and IVD regeneration. (biomedcentral.com)
  • Successful development of cell replacement therapies and IVD regeneration is crucially dependent on an in-depth understanding of cellular and molecular characteristics of the functional IVD. (biomedcentral.com)
  • Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
  • Second, we will use p63 knockout mouse models and lineage tracing experiments to determine the contribution of p63+ stem and progenitor cells during SG regeneration and in response to irradiation induced damage. (grantome.com)
  • Furthermore, we will define p63 dependent SG cellular identities and the defined cellular and molecular signature that is associated with regeneration and in response to irradiation by performing single cell RNA-sequencing (Aim2). (grantome.com)
  • These studies will better elucidate the role of ?Np63 in SG organogenesis, and adult gland maintenance, and elucidate its contribution towards SG regeneration and in response to irradiation induced injury. (grantome.com)
  • Importantly, our genetic and genomic studies will also uncover novel ?Np63-pathways dependent and independent biomarkers and drivers of the distinct cell states associated with regeneration and radiosensitivity. (grantome.com)
  • Guz Y, Nasir I, Teitelman G. Regeneration of pancreatic b-cells from intra-islet precursor cells in an experimental model of diabetes. (ejournals.ca)
  • There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)
  • During postnatal nephrogenesis, Troy+ cells are present in the cortex and papilla and display an immature tubular phenotype. (knaw.nl)
  • In work performed by Division of Translational Cancer Research it is shows that the hypoxic state pushes neuroblastoma and breast cancer towards an immature, stem cell-like phenotype. (lu.se)
  • This condition arises from immature T cells in more than 80% of cases and immature B cells in the remainder of cases. (medscape.com)
  • abstract = "Stem cells of adult regenerative organs share a common goal but few established conserved mechanisms. (syr.edu)
  • Neither the title of the paper, nor the abstract mentioned that in the experiment, five out of 25 rats receiving the transplant died with "teratoma-like tumors" in their brains, a well-known hazard of ES cells. (i-sis.org.uk)
  • HSCs make up a very small population of the hematopoietic system, however, these cells are invaluable as they have the potential to give rise to all mature blood and immune cell types and sustain life-long blood production. (stemcell.com)
  • Also making up a part of the hematopoietic system are short-term repopulating progenitor cells, which give rise to lineage-specific cell types. (stemcell.com)
  • Short telomere syndromes are the most prevalent premature aging disorders, with prominent phenotypes affecting the lung and hematopoietic system. (cdc.gov)
  • Profiling of a set of novel NP markers ( CD24 , CA12 , PAX1 , PTN , FOXF1 and KRT19 mRNA) in a representative set of subclones substantiated successful immortalization of multiple cellular subpopulations from primary isolates and confirmed their NP origin and/or phenotype. (biomedcentral.com)
  • however, relatively little is known about these NP cell subpopulations. (biomedcentral.com)
  • Here, we show that bone morphogenic protein (BMP)-mediated signaling is active in adult neural stem cells and is crucial to initiate the neurogenic lineage in the adult mouse subependymal zone. (cipsm.de)
  • Immortalized cells were clonally expanded and characterized based on collagen type I, collagen type II, α1 (COL2A1), and SRY-box 9 (SOX9) protein expression profiles, as well as on expression of a subset of established in vivo NP cell lineage markers. (biomedcentral.com)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • Hematopoietic stem cell lineage choices are decided by genetic networks that are turned ON/OFF in a switch-like manner. (lu.se)
  • This is generally a useful marker for cell dosing although there is some evidence that the CD34+ quantification may not be reliable in some circumstances. (wikipedia.org)
  • To measure neurogenesis, rats were given injections of the mitotic marker bromodeoxyurdine to label dividing cells in the hippocampus. (duke.edu)
  • Here, we evaluate Troy/TNFRSF19 as a segment-committed nephron progenitor cell marker. (knaw.nl)
  • Conversely, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and was shown to be an independent positive prognostic marker (11-14). (dbs-india.com)
  • Early passage NP monolayer cell cultures were initially characterized using a recently established NP marker set. (biomedcentral.com)
  • Investigators at the Department of Clinical Genetics have a strong track record in identifying novel cell surface markers on leukemia stem cells and were the first to demonstrate the potential therapeutic use of recombinant antibodies directed against the marker IL1RAP (interleukin-1 receptor accessory protein) on cancer stem cells in chronic myeloid leukemia (CML) and acute myeloid leukemia (AML). (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • In parallel, the expression of basal cell marker(KRT5) was partially modulated by nZnO exposure. (cdc.gov)
  • Examination of age-dependent effects of fetal ethanol exposure on behavior, hippocampal cell counts, and doublecortin immunoreactivity in rats. (mbfbioscience.com)
  • The broad interest of our group lies in understanding the molecular differences between fetal and adult hematopoiesis . (lu.se)
  • By using in-depth mass spectrometry-based quantitative proteomics to monitor protein levels in a comprehensive and quantitative manner, our ongoing research aims at defining the fetal- and adult-specific proteomic programs that regulate normal and malignant hematopoiesis. (lu.se)
  • This work also highlights the physiological role of cell competition in regulating mammalian organ size. (phys.org)
  • The adult mammalian intestinal epithelium is unique for its rapidly self renewing property. (biomedcentral.com)
  • Here we show a novel function for Retinoblastoma family member, p107 in controlling stem cell expansion in the mammalian brain. (rupress.org)
  • Identification of binding sites of EVI1 in mammalian cells. (rochester.edu)
  • Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
  • Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
  • The strategy used to identify new therapeutic markers on leukemia stem cells will also be explored in other tumor types studied within the MAD for Cancer Program. (lu.se)
  • Prenatal choline supplementation enhanced several properties of basal adult hippocampal neurogenesis (cell division and survival, neural stem/progenitor cell phenotype and proliferative capacity, trophic support), and this increase was associated with improvements in spatial working memory retention in a delayed-matching-to-place water maze task. (duke.edu)
  • The neurosphere-forming cells, named GEPCOT cells, were short-lived and highly proliferative in the brain. (elifesciences.org)
  • however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
  • Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
  • Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
  • As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)
  • To address these issues, the present set of experiments investigated how prenatal choline availability modulates specific properties of neurogenesis in the adult brain (in the intact brain and in response to injury), as well as hippocampal markers known to change in response to excitotoxin-induced seizures, and sought to relate changes in neurogenesis and in neuropathological markers following injury to changes in performance on spatial learning and memory tasks. (duke.edu)
  • Prenatal choline supplementation also attenuated other markers of hippocampal neuropathology shortly after seizures and promoted the long-term hippocampal recovery from seizures months after injury, including rescuing declines in adult hippocampal neurogenesis and in spatial memory performance in a standard water maze task. (duke.edu)
  • Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
  • term pre-GEPCOT cells (based on an acronym of the markers used to isolate the cells), were long-lived and quiescent, but they lacked the ability to form colonies in culture. (elifesciences.org)
  • Additional markers can be used to distinguish HSPC subsets within the CD34+ population and isolate HSPCs with different engraftment abilities and capacities to expand or generate mature blood cells in culture. (stemcell.com)
  • However, markers for most segment-committed progenitor cells remain to be identified. (knaw.nl)
  • Therefore, we hypothesized that CRC cancer stem cell markers (CRCSC) will identify a group of patients at high risk for progression. (jcancer.org)
  • In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. (scirp.org)
  • Using immunocytochemistry, we show that differentiated cells express S100 and P75 markers. (scirp.org)
  • A flow cytometric analysis of these keratocytes revealed changes in their expression levels of the indicative stem cell markers, Connexin 43 (Cx43), Paired Box 6 (PAX6), B-lymphoma Moloney murine leukemia virus insertion region-1 (Bmi-1), and C-X-C Chemokine Receptor 4 (CXCR4) suggestive of a less-differentiated phenotype compared with expression levels in cells not exposed to CS/DS. (cardiff.ac.uk)
  • Within the MAD for Cancer Program, a previously unexplored combination of strategies will be used to identify new cell surface markers on AML stem cells. (lu.se)
  • 2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)
  • Cells were harvested at 24 hours or 7 days post exposure, to investigate changes in (a) inflammation, (b) oxidative stress-related, and (c) cell-type specific markers using qPCR and/or IF staining. (cdc.gov)
  • Exposure to high dose of nZnO induced a significant decrease in expression of club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1) markers at mRNA levels. (cdc.gov)
  • In contrast, prenatal choline deficiency had little effect on basal adult hippocampal neurogenesis, and no effect on spatial memory performance. (duke.edu)
  • These pericryptal myofibroblasts produce the Wnt signalling ligands which bind to Frizzled receptors as well as morphogenetic protein (BMP) antagonists gremlin 1(GREM1), gremlin 2 (GREM2) on basal epithelial stem cells and also can modulate notch signaling. (biomedcentral.com)
  • The luminal epithelium forms the ducts and the secretory alveoli, whereas the basal epithelium consists essentially of myoepithelial cells. (biologists.com)
  • Sox2: a possible driver of the basal-like phenotype in sporadic breast cancer. (dbs-india.com)
  • Maternal choline supplementation differentially alters the basal forebrain cholinergic system of young-adult Ts65Dn and disomic mice. (mbfbioscience.com)
  • However, prior to commitment, it has been cells, it is evident that GATA-1 and PU.1 are able to specify observed that many genes are expressed at intermediate or basal erythroid and myeloid cell fates (see [16] and references therein). (lu.se)
  • The adult human airway epithelium is comprised of four major cell types, basal, club, goblet, and ciliated cells. (cdc.gov)
  • Methods: Normal human bronchial epithelial cells (NHBEpC) (at ≥ 90% viability) were seeded on 12-well porous 0.4 µm- cell culture inserts in basal cell culture medium. (cdc.gov)
  • One sentence summary Murine hematopoietic stem cells display transcriptional heterogeneity that is quantitatively altered with age and leads to the age-dependent myeloid bias evident after inflammatory challenge. (biorxiv.org)
  • Hence, we provide evidence that the immortalization strategy that we applied is capable of detecting cell heterogeneity in the NP. (biomedcentral.com)
  • Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)
  • While irradiation can effectively treat brain tumors, this therapy also causes cognitive impairments, some of which may stem from the disruption of hippocampal neurogenesis. (scite.ai)
  • Taken together, these findings demonstrate a robust neuroprotective effect of prenatal choline supplementation that may be driven by enhanced adult hippocampal plasticity and trophic support prior to injury, and shed light on the mechanisms underlying how prenatal choline availability alters adult hippocampal neurogenesis, which may contribute to changes in memory capacity and precision both throughout life and following neural assault. (duke.edu)
  • Gebara E., Bonaguidi M., Beckervordersandforth R., Sultan S., Udry F., Gijs P., Lie C., Ming G., Song H., Toni N . "Morpho-functional characterization of adult neural hippocampal stem cells reveals two morphotypes of radial glia-like cells. (chuv.ch)
  • Braun S., Moss J., Toni N. , Jessberger S. "Programming hippocampal neural stem/progenitor cells into oligodendrocytes enhances remyelination in the adult brain after injury" Cell Reports , 2015 Jun 23;11(11):1679-85. (chuv.ch)
  • But, although the mutation frequency of genes was much lower in ES cells, mutant ES cells accumulated with time in culture. (i-sis.org.uk)
  • Amongst the intestinal Wnt target genes (13), we found the Gpr49/Lgr5 gene to be unique in that it marks small cycling cells at crypt bottoms. (hubrecht.eu)
  • Two other Wnt target genes, RNF43 and ZNRF3, encode stem cell-specific E3 ligases that downregulate Wnt receptors in a negative feedback loop (35). (hubrecht.eu)
  • Here, we identify genes regulating the generation and maturation of liver cells from iPSCs. (ca.gov)
  • The group are currently focused on the pivotal role that HOXA cluster genes play in the demarcation and possible emergence of haematopoietic stem cells (HSCs) and their role in cancer. (nottingham.ac.uk)
  • Pathobiology: Cells and Genes;Cellular and Molecular Haematology. (nottingham.ac.uk)
  • Microarray analysis reveals that inhibition of Notch signalling causes downregulation of stem cell-associated genes in parallel with decreased proliferation and increased numbers of ciliated cells and that organoids also respond to oestradiol and progesterone treatment in a physiological manner. (nature.com)
  • Furthermore, Notch is responsible for the regulation of known stem cell factor genes. (nature.com)
  • [ 1 ] ETP-ALL frequently has mutations in RUNX1 and/or ETV6 in addition to genes that are more commonly associated with myeloid neoplasms and are otherwise rare in T-cell lymphoblastic leukemia/lymphoma (such as FLT3 , IDH1/2 , TET2 , and DNMT3A mutations). (medscape.com)
  • Previous work by Karnoub revealed that MSCs respond to breast tumors akin to the way they react to a wound or infection and that these cells participate in the formation of the breast tumor stroma, the supporting network of cells and their secretions that exist in the microenvironment of cancer cells. (newswise.com)
  • Cancer stem cells are thought to be the most virulent cells that lie within the core of most tumors, and are believed to be responsible for the resurgence of tumors following chemotherapy treatment. (newswise.com)
  • Accumulating data suggests that many human tumors are organized in cellular hierarchies initiated and maintained by a small population of self-renewing so called cancer stem cells. (lu.se)
  • Cells with high expression of HIF-2 α have properties rendering the tumors aggressive behavior potentially being the neuroblastoma tumor stem cells. (lu.se)
  • [ 4 ] Adult cases are unusual, accounting for less than 1% of brain tumors. (medscape.com)
  • Cerebellar hemispheric tumors of older children and adults show similar imaging features, except that contrast enhancement is more variable than in young children. (medscape.com)
  • In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
  • Increasing evidence emphasize that a substantial variance of protein expression is conveyed at the levels of protein synthesis and degradation, and that post-transcriptional control of gene expression plays a critical role in multiple aspects of cell biology, including coordination of cellular processes and rapid alteration of cellular phenotype. (lu.se)
  • Based on these combined insights, we have established Lgr5/R-spondin-based culture systems that allow the outgrowth of single mouse or human Lgr5 stem cells into ever-expanding organoids. (hubrecht.eu)
  • The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
  • NOD-SCID-Gamma (NSG) mice) and measuring the presence of human blood cells in the blood or bone marrow (BM) after extended periods of engraftment, e.g., 20 weeks or longer. (stemcell.com)
  • In the present study we have isolated and characterized a MSCs population from the periosteum of human adult donors. (univpm.it)
  • Enhancement of human erythroid progenitor cell growth by media conditioned by a human t-lymphocyte line. (shengsci.com)
  • Recent studies have shown that soluble factors elaborated by human T lymphocytes enhance erythroid burst formation by human peripheral blood null cells. (shengsci.com)
  • Normal human colon consists of millions of crypts, each containing about 2000 cells. (biomedcentral.com)
  • Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
  • The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
  • Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
  • In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
  • Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
  • LIM homeobox 2 promotes interaction between human iPS-derived hepatic progenitors and iPS-derived hepatic stellate-like cells. (ca.gov)
  • In this study, we developed a model system for improving interactions between human iPS-derived hepatic progenitor cells (iPS-HPCs) and human iPS-derived hepatic stellate cell-like cells (iPS-HSCs). (ca.gov)
  • Hepatocytic maturation in iPS-HPCs was significantly increased in direct co-culture with iPS-HSCs overexpressing LHX2, but not in co-culture with a human hepatic stellate cell line (LX-2) overexpressing LHX2. (ca.gov)
  • Expression of sox2 and oct4 and their clinical significance in human non-small-cell lung cancer. (dbs-india.com)
  • NP cells were immortalized by simian virus 40 large T antigen (SV40LTag) and human telomerase reverse transcriptase expression. (biomedcentral.com)
  • Here we report on the establishment of long-term, stable 3D organoid cultures from human fallopian tubes, indicative of the presence of adult stem cells. (nature.com)
  • These results show that for the first time, human endometrial stem cells can be differentiated into Schwann cells in 2D and 3D culture. (scirp.org)
  • A Murine Model of Variant Late Infantile Ceroid Lipofuscinosis Recapitulates Behavioral and Pathological Phenotypes of Human Disease. (mbfbioscience.com)
  • Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)
  • The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
  • To date, four types of human dental stem cells have been isolated and characterized: (i) dental pulp stem cells (DPSCs) 12 (ii) stem cells from exfoliated deciduous teeth (SHED) 21 (iii) stem cells from apical papilla (SCAP) 31 (iv) periodontal ligament stem cells (PDLSCs) 28 . (bvsalud.org)
  • Overall, our findings highlight the importance of TEs as regulatory agents and their dynamic activity during development, adult life, and disease in the human brain. (lu.se)
  • To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)
  • Human Papillomavirus and Risk of Head and Neck Squamous Cell Carcinoma in Iran. (who.int)
  • A major focus of the lab is to identify and study stem and progenitor cell populations in the sea anemone Nematostella vectensis. (scholarshipdb.net)
  • They demonstrated that transferring functional mitochondria of bone marrow-derived stem cells to defective parenchymal cells increases the aerobic respiration capacity of recipient mitochondria ( 7 ). (frontiersin.org)
  • Engraftment, clinical, and molecular follow-up of patients with multiple myeloma who were reinfused with highly purified CD34+ cells to support single or tandem high-dose chemotherapy. (shengsci.com)
  • Eighty-two patients with advanced multiple myeloma (MM) were enrolled in 2 sequential clinical studies of 1 or 2 courses of myeloablative therapy with stem cell support. (shengsci.com)
  • Development of pre-clinical models using stem cell technologies. (nottingham.ac.uk)
  • Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • We have chosen to concentrate on the emerging therapeutics that broadly involves a wide range of cell types in clinical trials registered on the National Institutes of Health's clinical trials web site. (biomedcentral.com)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • PheWAS of LTL identified 67 distinct clinical phenotypes associated with both short and long LTL. (cdc.gov)
  • The 2016 World Health Organization (WHO) classification of lymphoid neoplasms incorporates genetic data, clinical features, cell morphology, and immunophenotype, all of which have important implications for disease prognosis and management. (msdmanuals.com)
  • Karnoub's lab investigates the roles that mesenchymal stem cells (MSCs) play in the development and metastasis of breast cancer. (newswise.com)
  • Despite expanding knowledge of the role of MSCs to breast malignancy, the underlying molecular responses of breast cancer cells to MSC influences has not been fully delineated. (newswise.com)
  • The induction by MSCs of one such miRNA, miR199a, facilitated the acquisition of malignant properties by the cancer cells, including cancer stem cell and metastatic traits. (newswise.com)
  • After we found that miRNA-199a instigated in the cancer cells by MSCs was indeed promoting these cancer stem cells phenotypes and was facilitating cancer metastasis, we probed the mechanistic details of miR-199a's actions, " explains Karnoub. (newswise.com)
  • We are one step closer to understanding how cells in the tumor microenvironment, such as MSCs, promote the malignancy of neighboring cancer cells," says Karnoub. (newswise.com)
  • This leaves one with the distinct impression that adult stem cells might not exist, and furthermore, they may be a health hazard. (i-sis.org.uk)
  • Prenatal choline supplementation also enhanced olfactory bulb neurogenesis without altering cell proliferation in the subventricular zone, while prenatal choline deficiency had no effect on either measure, showing for the first time that prenatal choline's effects on adult neurogenesis is similarly expressed in another distinct neurogenic region of the adult brain. (duke.edu)
  • During kidney development, progressively committed progenitor cells give rise to the distinct segments of the nephron, the functional unit of the kidney. (knaw.nl)
  • We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ) NICs as Glast mid EGFR high PlexinB2 high CD24 −/low O4/PSA-NCAM −/low Ter119/CD45 − (GEPCOT) cells. (elifesciences.org)
  • A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
  • When cells from the subventricular zone are cultured in a way that allows the cells to freely float around (rather than growing on a surface), a few percent form spherical colonies called neurospheres. (elifesciences.org)
  • have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)
  • The adult subventricular zone (SVZ) stem cell niche has proven vital for discovering neurodevelopmental mechanisms and holds great potential in medicine for neurodegenerative diseases. (ox.ac.uk)
  • We were the first to link Wnt signaling with adult stem cell biology, when we showed that TCF4 gene disruption leads to the abolition of crypts of the small intestine (8), and that TCF1 gene knockout severely disables the stem cell compartment of the thymus (2). (hubrecht.eu)
  • In a study published in Developmental Cell , a group led by Prof. Wu Qingfeng from the Institute of Genetics and Developmental Biology (IGDB) of the Chinese Academy of Sciences demonstrated the presence of cell competition between neural progenitor cells (NPCs) during neurodevelopment. (phys.org)
  • Our cell lines yield novel insights into NP biology and provide promising new tools for studies of IVD development, cell function and disease. (biomedcentral.com)
  • Frontiers in cell and developmental biology. (lu.se)
  • CD34 is expressed in roughly 20% of murine haematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • Xue-Lian Sun et al, Stem cell competition driven by the Axin2-p53 axis controls brain size during murine development, Developmental Cell (2023). (phys.org)
  • PDGFRα demarcates the cardiogenic clonogenic Sca1+ stem/progenitor cell in adult murine myocardium. (ox.ac.uk)
  • Histologic subtypes of medulloblastoma include: (1) desmoplastic/nodular type, (2) medulloblastoma with extensive nodularity, and (3) large-cell/anaplastic medulloblastoma. (medscape.com)
  • [ 1 ] Several subtypes of medulloblastoma have been recognized including: (1) desmoplastic/nodular type, (2) medulloblastoma with extensive nodularity, (3) large-cell variant, and (4) anaplastic medulloblastoma. (medscape.com)
  • Although several subtypes of T-cell lymphoblastic leukemia/lymphoma exist, early T-cell precursor lymphoblastic leukemia (ETP-ALL) is the only subtype recognized as an entity in the revised 2016 WHO tumor classification. (medscape.com)
  • Non-ETP subtypes of T-cell lymphoblastic leukemia/lymphoma, in contrast, are associated with activating NOTCH1 mutations in over half of all patients and an additional 10% to 15% of cases have FBXW7 mutations, which also result in increased NOTCH signaling. (medscape.com)
  • Long-term hematopoietic stem cells (LT-HSCs) maintain hematopoietic output throughout an animal's lifespan. (biorxiv.org)
  • Here, we show that young and aged LT-HSCs respond differently to inflammatory stress, such that aged LT-HSCs produce a cell-intrinsic, myeloid-biased expression program. (biorxiv.org)
  • Using single-cell RNA-seq, we identify a myeloid-biased subset within the LT-HSC population (mLT-HSCs) that is much more common amongst aged LT-HSCs and is uniquely primed to respond to acute inflammatory challenge. (biorxiv.org)
  • Aged LT-HSCs demonstrate a cell-intrinsic myeloid bias during inflammatory challenge. (biorxiv.org)
  • The phenotype of iPS-HSCs, including gene and protein expression profiles and vitamin A storage, resembled that of hepatic stellate cells. (ca.gov)
  • Next, we generated iPS cell lines overexpressing LIM homeobox 2 (LHX2), which suppresses myofibroblastic changes in HSCs in mice. (ca.gov)
  • In conclusion, this study provides an evidence that LHX2 upregulation in iPS-HSCs promotes hepatocytic maturation of iPS-HPCs, and indicates that genetically modified iPS-HSCs will be of value for research into cell-cell interactions. (ca.gov)
  • However, eradication of the leukaemia repopulating cell or stem cell whilst retaining normal HSCs remains a challenge with current experimental therapies. (nottingham.ac.uk)
  • In pathological conditions, damaged cells transfer dysfunctional mitochondria toward recipient cells to ask for help and take up exogenous functional mitochondria to alleviate injury. (frontiersin.org)
  • However, generating mature and functional cell types from iPSCs is still challenging. (ca.gov)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
  • Targeted deletion of the Hoxa cluster affects B lymphopoiesis through depletion of early lymphoid progenitors. (nottingham.ac.uk)
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • The SBDS gene may not be required for neutrophil maturation but may act to maintain survival of granulocyte precursor cells. (medscape.com)
  • Lymphoblastic leukemias/lymphomas are neoplasms of precursor T cells and B cells or lymphoblasts. (medscape.com)
  • however, because lymphoblastic lymphoma and acute lymphoblastic leukemia (ALL) are known to represent the same disease entity, the World Health Organization (WHO) classification has unified these entities as precursor B-cell and T-cell lymphoblastic leukemia/lymphoma. (medscape.com)
  • Since endometrial stromal cells are easy to isolate, and expand rapidly from patients without leading to major ethical and technical problems. (scirp.org)
  • In APC-deficient colon carcinoma cells, we demonstrated that ß-catenin accumulates and is constitutively complexed with the TCF family member TCF4, providing a molecular explanation for the initiation of colon cancer (5). (hubrecht.eu)
  • Wu and his team systematically investigated the potential regulators, spatial properties, molecular features, mechanism and physiological role of cell competition between NPCs. (phys.org)
  • To gain deeper insight, the researchers generated phenotype-, genotype- and transcriptotype-dependent datasets through single-cell and bulk RNA sequencing, developed a loser signature scoring system and identified the molecular characteristic of loser cells. (phys.org)
  • This work elucidates the drivers, properties, molecular features and physiological function of cell competition in the context of neural stem/progenitor cells during early neurodevelopment. (phys.org)
  • Complex cell movements, molecular signaling, and interplay of transcription factors underlie these processes. (ox.ac.uk)
  • Immunohistochemical molecular phenotypes of gastric cancer based on SOX2 and CDX2 predict patient outcome. (dbs-india.com)
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
  • Injection of CD34+ hematopoietic stem cells has been clinically applied to treat various diseases including spinal cord injury, liver cirrhosis and peripheral vascular disease. (wikipedia.org)
  • Nowadays, more and more studies have revealed that cells in the cardiovascular system (such as cardiomyocytes, vascular smooth muscle cells, endothelial cells, et al. (frontiersin.org)
  • They can regulate stem cell functions by modulating effects of Wnt signalling on ephrin B1 (EFNB1) and its receptors EPHB2, EPHB3 in the stem cells [ 4 ]. (biomedcentral.com)