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  • genes
  • To select a Ref1-deficient TE671 clone, a starting pool of 10 6 cells was subjected to sequential infection at low MOI (0.01 to 0.001) with vesicular stomatitis virus G protein (VSV-G)-pseudotyped N-MLV vectors delivering drug resistance genes, followed by selection of transduced cells with the appropriate drug (Fig. 1 ). (asm.org)
  • To identify genetic factors linked to defective trans infection, Giovanna Rappocciolo, Ph.D., of the University of Pittsburgh and colleagues searched for patterns in gene expression, or the degree to which specific genes are turned on or off, in APCs from eight HIV nonprogressors and eight progressors enrolled in MACS. (nih.gov)
  • Compared to APCs from progressors, cells from nonprogressors expressed higher levels of several cholesterol-related genes associated with defective trans infection. (nih.gov)
  • IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. (cancerindex.org)
  • The genes to replace those that are defective in CMD are larger than most, so it has not been possible to apply the same methods successfully used for delivering other types of genes. (innovations-report.com)
  • Simply replacing the defective gene with a good laminin alpha-2 gene is not possible because its size makes it impossible for researchers to get it to squeeze inside viral vectors - disarmed viruses that are used to shuttle genes into cells. (innovations-report.com)
  • Vesicular Stoma
  • During several types of infections [such as lymphocytic choriomeningitis virus (LCMV), vesicular stomatitis virus (VSV), Listeria monocytogenes , and Toxoplasma gondii ], most of the effector CD8 T cells terminally differentiate and become short-lived, but a minority maintain memory potential and are capable of self-renewal ( 9 - 14 ). (pnas.org)
  • Adeno-assoc
  • Adeno-associated Virus- small, single stranded DNA that insert genetic material at a specific point on chromosome 19 From parvovirus family- causes no known disease and doesn't trigger patient immune response. (issuu.com)
  • But the team found a good stand-in in a similar protein called agrin that when miniaturized could be inserted inside an adeno-associated virus (AAV) vector. (innovations-report.com)
  • mutants
  • Cells infected with mutants defective in the helicase-primase or origin binding protein are unable to form stage III foci. (asm.org)
  • Furthermore, by analyzing viral DNA synthesis and the nucleus-associated viral DNA level, the results clearly showed that, although all three C-terminal mutants inhibited viral reverse transcription to different extents, the KK240,4AE mutant exhibited most profound effect on this step, whereas KK215,9AA significantly impaired viral DNA nuclear import. (biomedcentral.com)
  • Interestingly, only 3 of the 21 samples that were positive for mumps RNA were positive with both sets of primers, indicating that the persistence of mumps virus in the myocardium may be related to the selection of defective virus mutants. (ahajournals.org)
  • systemic
  • By using a miniature gene, similar in function to the one defective in CMD, and applying a newly developed method for "systemic" gene delivery, the Pitt researchers have shown that gene therapy for muscular dystrophy is both feasible and effective in a mouse model of especially profound disease. (innovations-report.com)
  • genetic
  • As a step towards understanding the genetic determinants of Ref1, we subjected Ref1-positive TE671 cells to three sequential rounds of selection with N-MLV reporter viruses. (asm.org)
  • Problems with a key group of enzymes called topoisomerases can have profound effects on the genetic machinery behind brain development and potentially lead to autism spectrum disorder (ASD), according to research announced today in the journal Nature. (blogspot.com)
  • gene
  • During early phase of the HIV-1 replication cycle, after virus entry into target cells, another pol gene product, reverse transcriptase (RT), copies viral genomic RNA into double-stranded cDNA which exists within a nucleoprotein preintegration complex (PIC). (biomedcentral.com)
  • proteins
  • Virus: Incomplete RNA virus, dependent on HBV envelope proteins. (natap.org)
  • ND10 proteins have been associated with the control of cellular growth, transcription, apoptosis, and the life cycles of several viruses ( 5 , 11 , 14 , 35 ). (asm.org)
  • viral particle
  • This hypothesis, tenaciously postulated by Dr. Rizzetto, was confirmed some years later by experimentally infecting chimpanzees and obtaining a new viral particle which was given the name of Hepatitis D Virus (HDV). (natap.org)
  • prevention
  • Increased knowledge of when and how HIV is eliminated from the body could have profound implications for the prevention of HIV transmission as well as treatment and vaccine development, according to NIH scientists. (nih.gov)
  • occurs
  • Restriction by Ref1 occurs at the level of reverse transcription, the major defect being a profound decrease in the production of full-length cDNA, while Fv1 acts after reverse transcription is complete ( 2 , 12 , 18 ). (asm.org)
  • Defective melanization of melanosomes occurs in oculocutaneous albinism associated with CHS. (medscape.com)
  • absence
  • We are studying HIV+ elite controllers, a select subset of patients who are able to control the virus in the absence of therapy. (yale.edu)
  • found
  • The inventors have found that the combination of hydroxyurea (HU), a nucleoside analog, and a protease inhibitor is capable of reducing the presence of the virus in both plasma and lymph nodes. (google.com.au)