• Diagnostic subsets of coexpressed genes reflected signaling activity, cross talk, and overlap of multiple mitogen-activated protein kinase (MAPK) pathways. (nih.gov)
  • Here, we deciphered the CRE1 regulatory range in the model cellulose and hemicellulose-degrading fungus Trichoderma reesei (anamorph of Hypocrea jecorina ) by profiling transcription in a wild-type and a delta- cre1 mutant strain on glucose at constant growth rates known to repress and de-repress CCR-affected genes. (biomedcentral.com)
  • Among CRE1 repressed transcripts, genes encoding unknown proteins and transport proteins were overrepresented. (biomedcentral.com)
  • The chlorophyll a/b-binding protein gene, otherwise known as the CAB gene, is one of the most thoroughly characterized clock-regulated genes in plants. (wikipedia.org)
  • The genes code for proteins that associate with chlorophyll and xanthophylls. (wikipedia.org)
  • Skeletal muscle development is orchestrated by the myogenic regulatory factor MyoD, whose activity is blocked in myoblasts by proteins preventing its nuclear translocation and/or binding to G/C-centered E-boxes in target genes. (wustl.edu)
  • Integrated analysis of differential gene expression, previously reported quantitative trait loci, and genome-wide association studies indicated that TRIB3 , SAA ( SAA1 , SAA3 , and M-SAA3.2 ), VEGFA, PTHLH , and RPL23A were the most promising candidate genes affecting milk protein and fat percentage. (biomedcentral.com)
  • RNA-seq identifies a novel set of TEAD4 target genes encoding muscle structural and regulatory proteins and those required for the unfolded protein response. (nih.gov)
  • Together these results show that TEAD factor activity is essential for normal C2C12 cell differentiation and define a novel and non-redundant role for TEAD4 in regulating expression of the unfolded protein response genes. (nih.gov)
  • A bZIP transcription repressor of mitochondrial function (CIN5) positively regulated Pdr1 while hundreds of genes encoding mitochondrial proteins were confirmed as negative regulators of Pdr1. (bvsalud.org)
  • The established cerebrolysin peptides were shown to be competitive inhibitors of the seven targeted proteins. (ima-press.net)
  • As a German botanist who focused on molecular plant physiology, Klaus Kloppstech discovered the potential regulation of circadian rhythm in peas by transcripts of chlorophyll a/b binding protein (LHCB/CAB). (wikipedia.org)
  • p class=\'abstract\'>Lin28/LIN-28 is a conserved RNA-binding protein that promotes proliferation and pluripotency and can be oncogenic in mammals. (stanford.edu)
  • Here, we investigate the structural basis for RNA recognition by ProQ/FinO proteins, through the crystal structure of the ProQ/FinO domain of the Legionella pneumophila DNA uptake regulator, RocC, bound to the transcriptional terminator of its primary partner, the sRNA RocR. (nature.com)
  • A general property of disordered proteins is their structural expansion that results in a high macromolecular flexibility. (lu.se)
  • Besides genetic data, gene expression profiles and metabolic pathway analysis offer new opportunities to elucidate the underlying mechanisms of complex traits in humans and farm animals. (biomedcentral.com)
  • These results provide a resource of 600 human proteins containing effectors and demonstrate a scalable strategy for assigning functions to protein domains. (stanford.edu)
  • those of inhibitors of seven targeted human proteins (the protein kinases MAPK1, VPRBP, and PKC, the gamma-secretase PS1, the kinases CDK1, SGK1 and mTOR). (ima-press.net)
  • A protein called CAB GATA factor 1 (CGT-1) binds to the GATA repeats and promotes CAB2 production. (wikipedia.org)
  • Inhibitors of anti-apoptotic BCL-2 family proteins in combination with chemotherapy and hypomethylating agents (HMAs) are promising therapeutic approaches in acute myeloid leukemia (AML) and high-risk myelodysplastic syndromes (MDS). (haematologica.org)
  • More recently, the application of RNA-seq technologies has enabled the elucidation of the biological partners and targets of several ProQ/FinO family proteins. (nature.com)
  • I'm using cell and molecular biology techniques, biochemistry as well as microscopy to characterise the plant SUN proteins. (brookes.ac.uk)
  • SCRIPTS: ~~~~~~~~ present.tcl -- the master script, which sets up sets up the VMD presentation styles and colors, loads lac repressor + water, calls the other scripts (which create the Tk menus and load the PDB data for the elastic loop molecular sets), and resets the presentation to its initial state. (uiuc.edu)
  • MRD is among the few methods that can provide molecular-level informa- tion about protein solvation in native as well as denatured states, and it is used here to simultaneously monitor the interactions of urea and water with the unfolding protein. (lu.se)
  • 1995), the molecular mechanism and the peptide group, suggesting that urea-peptide inter- whereby urea unfolds proteins has not been established. (lu.se)
  • In Weber's lab, Silva studied the plasticity of proteins and supramolecular structures and their physiological consequences. (biophysics.org)
  • We find a relationship between repressor function and evolutionary age for the KRAB domains, discover that Homeodomain repressor strength is collinear with Hox genetic organization, and identify activities for several domains of unknown function. (stanford.edu)
  • This high-resolution snapshot of genetic profiles of 1710 GC sequences, through a comparison with 2018 data (1479 GC sequences) within the sentinel system, highlights change in proportions and expansion of select GC strains and the associated genetic mechanisms of resistance. (cdc.gov)
  • Deep mutational scanning of the CRISPRi KRAB maps the co-repressor binding surface and identifies substitutions that improve stability/silencing. (stanford.edu)
  • Mutational profiling of MDS samples identified ASXL1 mutations as predictors of response. (haematologica.org)
  • Protein complexes, which are formed as a result of these interactions, consist of two or more components that associate along specific pathways - protein association pathways. (lu.se)
  • The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. (nature.com)
  • Protein interactions of the transcription factor Hoxa1. (nih.gov)
  • For this study, we are discussing new challenges in using chromatin immunoprecipitation (ChIP) assay to confirm protein - DNA interactions and identifying interacting proteins by mass spectrometry. (okstate.edu)
  • We recently reported a procedure for inferring gene-regulatory network models by integrating gene-expression profiles with high-throughput measurements of protein interactions [ 18 ]. (biomedcentral.com)
  • Many of these models contain transcriptional interactions for which the regulatory effects (inducer versus repressor) are ambiguous and cannot be determined from publicly available expression profiles. (biomedcentral.com)
  • The eponymous FinO protein was discovered as a regulator of F plasmid conjugation nearly 50 years ago, and acts to bind a single partner sRNA called FinP to stabilize FinP and facilitate its interactions with its antisense partner, the mRNA encoding the major F plasmid transcription factor, TraJ 5 . (nature.com)
  • 1 nsec) interactions of urea and water with the native and denatured protein. (lu.se)
  • Solvent denaturation is a result of altered protein-solvent vorably with water-peptide interactions. (lu.se)
  • If this is the case, interactions, but it is not clear whether denaturants like urea then solvent denaturation can be driven simply by the ex- act directly by binding to the protein surface or indirectly by posure of more binding sites in the denatured protein perturbing solvent-mediated hydrophobic interactions or by (Schellman 1987). (lu.se)
  • The complex role of IRE1 in the regulation of mammalian UPR cannot be fully explained by IRE1's one known mRNA target, X box‐binding protein‐1 ( XBP1 ), or through its RIDD activity. (biomedcentral.com)
  • Likewise, the ProQ/FinO domain-containing protein RocC of Legionella pneumophila interacts with only one trans -acting sRNA (RocR) to repress post-transcriptionally multiple mRNA targets 6 . (nature.com)
  • Unexpectedly, disruption of many 60S ribosomal proteins also activated Pdr1, thus mimicking the effects of the mRNA translation inhibitors. (bvsalud.org)
  • Expression of the TEA/ATTS DNA binding domain that acts a dominant negative repressor of TEAD factors in C2C12 myoblasts inhibits their differentiation, while selective shRNA knockdown of TEAD4 results in abnormal differentiation characterised by the formation of shortened myotubes. (nih.gov)
  • In the last decade, diagnostic and prognostic evaluation has been facilitated by global gene expression profiling (GEP), provid- ing a new powerful means for the classification, prediction of survival, and response to treatment of lym- phomas. (lu.se)
  • The Cab gene, responsible for encoding the chlorophyll a/b-binding protein in Arabidopsis, plays a crucial role in the absorption of excitation energy necessary for photosynthetic mechanisms in plants. (wikipedia.org)
  • Nevertheless Hair mostly regulates the appearance of a big group course Ib or NrdR comprises an N-terminal nonclassical zinc-binding area and a distinctive C-terminal ATP-cone domains [1] which classifies NrdR inside the ATP-cone category of proteins. (immune-source.com)
  • We later identified PIAS (protein inhibitor of activated STAT)xalpha as a DJ-1-binding protein, and found that DJ-1 restored androgen receptor (AR) transcription activity that was repressed by PIASxalpha. (nih.gov)
  • To further characterize the function of DJ-1, we cloned cDNA encoding a novel DJ-1-binding protein, DJBP, by a yeast two-hybrid system. (nih.gov)
  • Recent evidence indicates that muscle gene expression is also regulated at the cis level by differential affinity for DNA between MyoD and other E-box binding proteins during myogenesis. (wustl.edu)
  • Here, we used cell-based and in vivo Drosophila, Xenopus laevis, and mouse models to show that ZEB1, a G/C-centered E-box binding transcriptional repressor, imposes a temporary stage-dependent inhibition of muscle gene expression and differentiation via CtBP-mediated transcriptional repression. (wustl.edu)
  • This hypermethylation results in recruitment of methyl CpG binding protein 2 (MeCP2) to hypermethylated CpG dinucleotides. (okstate.edu)
  • IRE1's unique feature is its UPR-related RNase activity that is responsible for the production of a potent transcription factor, X box‐binding protein‐1 (XBP1s). (biomedcentral.com)
  • The tox gene is regulated by a corynebacterial iron-binding repressor (DtxR). (medscape.com)
  • 17O and 2H magnetic relaxation dispersion (MRD) to study urea-induced denaturation of intestinal fatty acid-binding protein (I-FABP). (lu.se)
  • 10 nsec) to the protein even in 7.5 M urea, where the large internal binding cavity is disrupted and CD indicates a fully denatured protein. (lu.se)
  • In particular, inhibition of PKC and mTOR stimulated the increased autophagy (the utilization of waste and abnormal proteins), which contributes to an increase in the survival of cells and model organisms. (ima-press.net)
  • Alvocidib, a cyclin-dependent kinase 9 (CDK9) inhibitor and indirect transcriptional repressor of the anti-apoptotic factor MCL-1, has previously shown clinical activity in AML. (haematologica.org)
  • Cell surface expression of the ROMK (Kir 1.1) channel is regulated by the aldosterone-induced kinase, SGK-1, and protein kinase A. J Biol Chem. (ima-press.net)
  • Expression of ALK in malignant T cells is typically due to the t(2;5) translocation resulting in formation of the fusion gene which encodes a 80-kDa hybrid protein that contains portion of the nuclear protein nucleophosmin (NPM) joined to the entire cytoplasmic portion of the receptor tyrosine kinase ALK. (upenn.edu)
  • Right here we profile bacterial destiny under circumstances of overexpression and deletion of NrdR in appearance causes a substantial decrease in bacterial development and fitness also at normal temperature ranges and causes lethality at raised temperature ranges. (immune-source.com)
  • The role of SUN3 in the ER is reinforced by the identification of a protein interaction between SUN3 and the ER membrane-bound transcription factor maMYB. (brookes.ac.uk)
  • EBNA3C is a large nuclear transcription factor involved in modulating transcription activated by a cellular repressor RBP-Jkappa and other transcription factors. (pennmedicine.org)
  • At the sub-cellular scale, maps document the spatial organization of proteins, RNA, DNA, and metabolites with nanometer precision and temporal acuity on the order of seconds. (stanford.edu)
  • Endoplasmic reticulum (ER) homeostasis is dependent on the proper folding and maturation of secretory pathway proteins, on lipid biosynthesis, and on cellular calcium homeostasis. (biomedcentral.com)
  • [ 1 ] More recently, Van den Velde and colleagues have suggested that species of corynebacteria would be more correctly identified based on their cellular fatty acid profiles (ie, for the C14 to C20 fatty acids). (medscape.com)
  • We uncovered the functions of ABCE proteins Rli1/ABCE1 and Arb1 in PIC assembly and ribosome biogenesis, and identified the tRNA methyltransferase Gcd10/Gcd14, which contributed to the discovery of the TRAMP-mediated RNA surveillance pathway. (nih.gov)
  • A fundamental understanding of protein stability and the mechanism of denaturant action must ultimately rest on detailed knowledge about the structure, solvation, and energetics of the denatured state. (lu.se)
  • We show that addition of microRNA supports neuronal maturation both in terms of gene and protein expression, as well as promotes the development of active ion channels and the ability of iNs to generate current induced and spontaneous action potentials. (nih.gov)
  • We used massive, parallel, high-throughput, RNA-seq to generate the bovine transcriptome from the mammary glands of four lactating Holstein cows with extremely high and low phenotypic values of milk protein and fat percentage. (biomedcentral.com)
  • In my presentation, I will summarize recent QENS and NSE results on the dynamics of the intrinsically disordered myelin basic protein (MBP) and the chemically denatured bovine serum albumin (BSA) (1,2,3). (lu.se)
  • Corroborating these immediate results global proteome evaluation pursuing NrdR overexpression demonstrated a significant reduction in global proteins appearance. (immune-source.com)
  • Metastasis-associated protein 2 is a repressor of estrogen receptor alpha whose overexpression leads to estrogen-independent growth of human breast cancer cells. (jefferson.edu)
  • My career-long interest revolves around the understanding of biological recogni-tion processes, especially how proteins correctly fold and interact with nucleic acids and how proteins undergo misfolding, related to neurode-generative diseases and cancer," he explains. (biophysics.org)
  • Protein performs its biological functions by interacting with other proteins. (lu.se)
  • MeCP2 protein has been shown to interact with corepressors to suppress or turn off gene transcription, however, in TNBS-induced colitis recruitment of MeCP2 to GLS1 promoter results in increased gene expression indicating interaction with coactivators rather than corepressors. (okstate.edu)
  • Fuurther, we performed the ChIP assay followed by bisulfite conversion and methylation specific PCR to confirm protein-DNA interaction. (okstate.edu)
  • On top of the elastic rods, one shows fitted all-atom DNA structures, electric field profiles, forces of protein-DNA interaction, and the CAP protein bound within the short loops. (uiuc.edu)
  • Contributions to neutropenia from PFAAP5 (N4BP2L2), a novel protein mediating transcriptional repressor cooperation between Gfi1 and neutrophil elastase. (nih.gov)
  • Launch For all microorganisms well-timed and temporal legislation of gene appearance and its own translation to proteins level is essential for cell proliferation. (immune-source.com)
  • Neutron Spin-Echo spectroscopy (NSE) on the other hand, offers the highest energy resolution in the field of neutron spectroscopy and allows the study of slow collective motions in proteins up to several hundred nanoseconds and in the nanometer length-scale. (lu.se)
  • Two studies on the transcriptome of the mammary gland of Holstein cows using an oligonucleotide microarray have been presented, one of which compared the gene expression profile before (dry) and after (milk) parturition, using an Affymetrix cDNA array [ 22 ]. (biomedcentral.com)
  • Here, we report SPOTlight, a versatile high-throughput technique to isolate individual yeast or human cells with unique spatiotemporal profiles from heterogeneous populations. (stanford.edu)
  • Here, we report that C. elegans LIN-28 represses the expression of LIN-46, a downstream protein in the heterochronic pathway. (stanford.edu)
  • The unfolded protein response is a survival signaling pathway that is induced during various types of ER stress. (biomedcentral.com)
  • Cerebrolysin can have geroprotective effects, by inhibiting the seven targeted proteins, by activating endorphinergic neurotransmission, and can be indirectly involved in vascular tone normalization. (ima-press.net)
  • These molecules are involved in cell division, metastasis, apoptosis, cell cycle regulation and regulation of protein degradation. (pennmedicine.org)