OrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
PimentaPiper nigrumGelsChromatography, High Pressure LiquidChromatography, AffinityChromatography, GelChromatographyChromatography, GasChromatography, Ion ExchangeChromatography, Thin LayerChromatography, LiquidMolecular Sequence DataAmino Acid SequenceKineticsMolecular WeightChromatography, DEAE-CelluloseMass SpectrometryMolecular StructureElectrophoresis, Polyacrylamide GelBase SequenceEscherichia coliGas Chromatography-Mass SpectrometrySubstrate SpecificityElectrophoresis, Gel, Two-DimensionalMagnetic Resonance SpectroscopyStructure-Activity RelationshipHydrogen-Ion ConcentrationBiological ProductsChromatography, AgaroseStereoisomerismSpectrophotometry, UltravioletChemistryCloning, MolecularAmino AcidsChemical Phenomena
Silica gelHPLCSynthesisMass spectrometryReagentCompoundsStationary phasePharmacologicalCoumarinMoleculeSolventDecomposition productsAldehydesEsterDeterminationDetectionAntitumorHydrolysisThereofTherapeuticElectrophoresisHighFlameReactionSpotsBiological activitiesAmino acidDetectStabilityNativeActivityThin-layerJournalPreparationColumnsSyntheticContentEffectTreatmentNaturalDevelopmentStudyIdentificationComparison
Silica gel17
  • The van URK-Salkowski reagent - a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives" (PDF). (wikipedia.org)
  • IE solvent-resistant chiral column, the amylose-tris (3,5-dichlorophenylcarbamate) covalently bonded to the surface of the silica gel. (thelabstore.co.uk)
  • CHIRALPAK IE-3 / SFC, CHIRALPAK IE / SFC uses a stationary phase in which an amylose derivative (Amylose tris (3,5-dichlorophenylcarbamate)) is immobilized on a silica gel carrier. (thelabstore.co.uk)
  • As a modifier, you can use all solvents that can be used for chromatographic columns such as methanol, ethanol, 2-propanol, acetonitrile, THF, halogen solvents and other silica gel-based chromatography columns. (thelabstore.co.uk)
  • Precoated silica gel 60 F 254 plates (Merck) for thin layer chromatography (TLC) were used. (hindawi.com)
  • Column chromatography (CC) was carried out using silica gel (Si) 60 mesh of 35-60 and 60-120 (E. Merck, Darmstadt, Germany). (hindawi.com)
  • The isolation and purification involved a combination of methods including silica gel column chromatography, Sephadex LH-20, and semi-prep HPLC separations. (umn.edu)
  • 1.2 This test method involves drawing air through a cartridge containing silica gel coated with 2,4-dinitrophenylhydrazine (DNPH) reagent. (astm.org)
  • 1.5 This test method instructs the user on how to prepare sampling cartridges from commercially available chromatographic grade silica gel cartridges by the application of acidified DNPH to each cartridge. (astm.org)
  • This flow rate limitation is principally due to the high pressure drop (>8 kPa at 1.0 L/min) across the user prepared silica gel cartridges which have a particle size of 55 to 105 μ m. (astm.org)
  • 1.7 Alternatively, pre-coated DNPH silica gel cartridges are also commercially available and may be substituted provided they can be demonstrated to perform equivalently. (astm.org)
  • Some of these use silica gel of a larger particle size that results in a lower pressure drop across the cartridge. (astm.org)
  • Separation of valerenic acid and its derivatives was performed by TLC using a ternary mobile phase of hexane-ethyl acetate-glacial acetic acid (65:35:0.5 v/v) on silica gel HF 254+366 plates. (scialert.net)
  • The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID. (lookformedical.com)
  • The compound is purified by chromatography on silica gel or by distillation. (peptidesforresearch.com)
  • Silica gel plates 60 F 254 (Merck) were used. (medline.ru)
  • Data on recovery rates for charcoal (16291966), silica-gel (10193369), chromosorb-103, Tenax-GC, and Porapak-Q sorbents is included. (cdc.gov)
HPLC3
  • 1992). Determination is via gas chromatography (GC) with mass spectrometry (MS), electron capture (ECD), nitrogen-phosphorus (NPD), flame photometric (PPD), or flame ionization (FID) detectors, high performance liquid chromatography (HPLC) with spectrophotometric detection, or thin layer chromatography (TLC). (cdc.gov)
  • The DNPH derivatives are analyzed for parent aldehydes and ketones utilizing high performance liquid chromatography (HPLC). (astm.org)
  • The determination of formaldehyde and other carbonyl compounds, as DNPH derivatives, is similar to that of U.S. EPA Method TO-11A in that it utilizes HPLC with UV detection as the analytical finish. (astm.org)
Synthesis12
  • Furthermore, this method provides a direct approach for the synthesis of fluorinated drug derivatives, which has important application for drug discovery and development. (nature.com)
  • Conversion of D-glucose to 4- C- hydroxymethyl-1,2- O -isopropylidene-α-D-ribofuranose, which is a key precursor for the synthesis of different types of bicyclic/spiro nucleosides, led to the formation of an inseparable 1:1 mixture of the desired product and 4- C- hydroxymethyl-1,2- O -isopropylidene-α-D-xylofuranose. (beilstein-journals.org)
  • The synthesis of the ribo - trihydroxy sugar derivative starting from diacetone-D-glucose led to the formation of an inseparable 1:1 mixture of the required compound and its C-3 epimer, i.e., 4- C- hydroxymethyl-1,2- O -isopropylidene-α-D-xylofuranose [10] . (beilstein-journals.org)
  • We herein report the synthesis of a derivative of the natural compound celastrol linked to the antimalarial drug primaquine through an amide obtained by the activation of the carboxylic acid with HOBt/EDC. (mdpi.com)
  • Isatin (1-H-indole-2,3- dione) and its derivatives possess diverse biological and pharmacological activities and are widely used as a starting material for the synthesis of a broad range of heterocyclic compound substrates for drug synthesis [ 1 ]. (ijpsonline.com)
  • The naturally occurring compounds show a range of biological activities, prompting the synthesis and study of related derivatives [15-17]. (1library.net)
  • Since our lab recently reported a synthesis of the natural product aristoquinoline [7] , we viewed the halichonic acids as ideal targets to extend the scope of our synthetic methodology. (beilstein-journals.org)
  • 100 references, on organotin(IV) complexes of the carboxylic acid derivatives are presented with special reference to their methods of synthesis, spectroscopic and structural studies and their biological activities. (degruyter.com)
  • The synthesis reaction is divided into the stages for the preparation of the 2-(hydroxymethyl)azanaphthalen-1-one 5 starting from the 2-bromobenzaldehyde 2-bromohydroquinone 3 and ethyl cyanoacetate 4 , the preparation of the ester derivative of the 1-azanaphthalene and the hydrolysis of the ester derivative to 2-(hydroxymethyl)azanaphthalen-1-one. (peptidesforresearch.com)
  • The 3,5-bis(trifluoromethyl)benzylidene derivative is used as an intermediate for the synthesis of the compound according to the invention. (peptidesforresearch.com)
  • The synthesis reaction of the compound in accordance with the invention is divided into the stage for the preparation of the 3,5-bis(trifluoromethyl)benzylidene derivative and the stage for the preparation of the 2-(azanaphthalen-1-yl)-methyl-sulfonate. (peptidesforresearch.com)
  • The modular synthesis is based on Suzuki cross-coupling of the aryl substituents as boronic acid precursors with 5,8-dibromo-2-( tert -butyl)-4,9-dimethoxy-2,3-dihydro-1H-phenalen-1-one, and the subsequent transformation of the product to the desired 2,5-diaryl 6-hydroxyphenalenone in a reduction/deprotection sequence. (thieme-connect.com)
Mass spectrometry1
Reagent2
  • Carbonyl compounds readily form stable derivatives with the DNPH reagent. (astm.org)
  • Fast Black K/1 N NaOH reagent gave red coloured spots with native zopiclone (Rf=0,34), violet-with alkaline hydrolysis product (Rf=0,63) and blue-violet spot (Rf=0,95) with 2-amino-5-chloropyridine. (medline.ru)
Compounds7
  • Seven compounds inhibited the growth of some cancer cell lines, whereas one of the 2-quinoline derivatives displayed favorable activity against all tested cancer cells with GI 50 values of 0.92-13 μM. (springer.com)
  • These findings prompted us to extend our search for biologically active compounds among nitrogen heterocyclic derivatives. (springer.com)
  • In order to obviate to said drawback, compounds more stable than hyperforin, such as the salts disclosed in WO 99/41220 and the hydroxy-functionalized derivatives (WO 99/64388) cited above, have recently been prepared. (justia.com)
  • The purity of the compounds was monitored by thin layer chromatography (TLC) and the structures of the products were confirmed by elemental and spectral analysis. (ijpsonline.com)
  • In our continued focus on 1-benzyl-3-(5-hydroxymethyl-2-furyl)indazole (YC-1) analogs, we synthesized a novel series of 3,9-substituted α-carboline derivatives and evaluated the new compounds for antiproliferactive effects. (1library.net)
  • A method of gel filtration chromatography using agarose, the non-ionic component of agar, for the separation of compounds with molecular weights up to several million. (lookformedical.com)
  • Optical rotation measurements confirmed that these synthetic compounds were in fact the enantiomers of the natural products, establishing both the relative and absolute configurations of the halichonic acids. (beilstein-journals.org)
Stationary phase2
Pharmacological2
  • In addition, we tested the anticerebral ischemia-reperfusion injury activity of these puerarin derivatives in order to determine their permeability through the blood-brain barrier and pharmacological activity. (hindawi.com)
  • In the last two decades, the essential oil and valerenic acid derivatives have become more important, not only from a pharmacological point of view, but also for the quality control and standardization of valerian phytomedicines (Hendriks et al . (scialert.net)
Coumarin4
  • A series of 3-[(2 E )-3-substituted-prop-2-enoyl]-2 H -chro men-2-one derivatives (II a-h) were prepared by Claisen-Schmidt condensation of 3-acetyl coumarin with aromatic aldehydes. (ijpsr.com)
  • Their utility stimulated the development of new synthetic routes for the preparation of coumarin derivatives. (ijpsr.com)
  • There are some examples of coumarin natural products that exert antidiabetic effect [ 1 , 3 ]. (hindawi.com)
  • Osthole, a coumarin derivative, reported as antidiabetic agent, alleviates hyperglycemia in db / db mice [ 3 ]. (hindawi.com)
Molecule2
  • An ethyl acetate and acetyl amino group were linked to the phenolic hydroxyl at the 7/4′ position of the puerarin molecule to produce 7-mono- and 7,4′-disubstituted derivatives of puerarin. (hindawi.com)
  • For the derivatives 1 and 2 suitable for single-molecule junctions, the corresponding oxophenalenoxyl radicals 1R and 2R were obtained by oxidation and analysed by electron paramagnetic resonance spectroscopy. (thieme-connect.com)
Solvent2
  • The 2-(azanaphthalen-1-yl)-methyl-sulfonate is reacted in the stage with methanesulfonic acid in the presence of an inert solvent with elimination of methyl sulfite and formation of the thionylate 6, in which the compound in accordance with the invention 2 -(azanaphthalen-1-yl-sulfonyl)propane is obtained as a by-product. (peptidesforresearch.com)
  • Aliquots of blood, urine samples and zopiclone hydrolysis products were first analyzed by thin-layer chromatography in solvent system benzene-1,4-dioxane-conc. (medline.ru)
Decomposition products3
  • These include the essential oil and its sesquiterpenoids (valerenic acid derivatives), epoxy iridoid esters (valepotriates) and their decomposition products such as baldrinal and homobaldrinal, amino acids (arginine, GABA, glutamine, tyrosine) and alkaloids (Upton et al . (scialert.net)
  • TLC data and retention indices of zopiclone and its decomposition products are presented in this article. (medline.ru)
  • The main purpose of our study was to detect native zopiclone, its decomposition products and 2-amino-5-chloropyridine in blood and urine by means thin-layer chromatography and gas chromatography (FID and ECD). (medline.ru)
Aldehydes2
  • These introduced aldehydes and ketones can then be modified with fluorescent or biotinylated hydrazine, hydroxylamine or amine derivatives to label the polysaccharide or glycoprotein. (thermofisher.com)
  • Alkenes from unsaturated fatty acids and ceramides can also be converted to glycols by osmium tetroxide and then oxidized by periodate to aldehydes, and periodate will oxidize certain β-aminoethanol derivatives such as the hydroxylysine residues in collagen, as well as methionine (to its sulfoxide) and certain thiols (usually to disulfides). (thermofisher.com)
Ester4
  • Betulinic, ursolic and oleanolic acids isolated from the aerial parts of Eriope blanchetii (Lamiaceae) were subjected to different esterification reactions, yielding 12 C-3 position ester derivatives. (mdpi.com)
  • The stage of the preparation of 2-(hydroxymethyl)azanaphthalen-1-one 5 and the stage of the preparation of the ester derivative of the 1-azanaphthalene are in particular described in Journal of the Chemical Society, Perkin Transactions, I, 1968, pages 3-15. (peptidesforresearch.com)
  • The hydrolysis of the ester derivative of the 1-azanaphthalene into 2-(hydroxymethyl)azanaphthalen-1-one 5 in accordance with the invention is described in Applied Chem. (peptidesforresearch.com)
  • 9~ yl)-1-methyl-ethoxymethyl]-phosphonic acid diisopropoxycarbonyloxymethyl ester (tenofovir disoproxil fumarate, Viread®) and (2R, 5S, cis)-4-amino-5- fluoro-l-(2~ hydroxymethyl-1,3-oxathiolan-5-yl)-(1H)-pyrimidin-2-one (emtricitabine, EmtrivaTM, (-)-cis FTC) and their physiologically functional derivatives. (gc.ca)
Determination1
  • Additionally, for a more accurate determination of total valerenic acid derivatives (including valerenic acid, hydroxyvalerenic acid and acetoxyvalerenic acid) extinction coefficient of each compound in ethanol was used. (scialert.net)
Detection1
  • Fast Black K /1 N NaOH and Dragendorff/Jodplatinate reagents can be used for zopiclone and its alkaline hydrolysis products detection. (medline.ru)
Antitumor2
  • The new 3,9-substituted α-carboline derivatives exhibited excellent anti-proliferative activities, and compound 11 can be used as a promising pro-apoptotic agent for future development of new antitumor agents. (1library.net)
  • A novel series of 2-amino and 2′-aminocombretastatin derivatives were synthesized and evaluated for antitumor activity. (lookchem.com)
Hydrolysis5
  • The purpose of our study was to identify zopiclone and its alkaline hydrolysis products by TLC, GC-FID and GC-ECD in blood and urine samples. (medline.ru)
  • 2-amino-5-chloropyridine, the main product of alkaline hydrolysis of zopiclone, should be a target substance during toxicological analysis of cases involving zopiclone. (medline.ru)
  • After cooling hydrolysis product was extracted by 25,0 ml of dichlo-romethane-acetone mixture (4:1). (medline.ru)
  • Zopiclone and its hydrolysis products were identified also by means GC. (medline.ru)
  • Zopiclone and its hydrolysis products were identified by means GC-ECD. (medline.ru)
Thereof2
  • The present invention relates to hyperforin and adhyperforin derivatives and the use thereof in the pharmaceutical and/or nutritional field, in particular in the treatment of depression and Alzheimer's disease. (justia.com)
  • The invention relates to 1-piperazine- and 1-homopiperazine-carboxylate derivatives, and to the preparation and therapeutic application thereof. (allindianpatents.com)
Therapeutic1
  • The present invention is also concerned with pharmaceutical compositions and formulations of said combinations of tenofovir disoproxil fumarate and emtricitabine, and their physiologically functional derivatives, as well as therapeutic methods of use of those compositions and formulations. (gc.ca)
Electrophoresis3
  • Thin-layer chromatography of dinitrophenyl derivatives prepared from Pronase-digested microsomal protein and sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis of solubilized microsomes indicated that the radiolabeled products were covalently bound to amino acid residues of microsomal protein. (aspetjournals.org)
  • Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (lookformedical.com)
  • Native gel electrophoresis revealed a rapidly-modulated reciprocal relationship between the burden of unfolded proteins and BiP oligomers and slower equilibration between oligomers and inactive, covalently-modified BiP. (elifesciences.org)
High2
  • For the SFC columns CHIRALPAK IE - 3 / SFC, CHIRALPAK IE / SFC have the strength required for SFC specified in the High Pressure Gas Safety Act, and we will attach documents to prove this to the product. (thelabstore.co.uk)
  • It has now been found that hyperforin and adhyperforin derivatives obtainable by reduction of all double bonds of the isoprene chains and/or by reduction of the keto groups at the 1- and 10-positions to hydroxy groups not only have high stability, but also possess antidepressant, anxiolytic and anti-neurodegenerative activities surprisingly higher than hyperforin and adhyperforin. (justia.com)
Flame1
  • The technique involved collection on a solid sorbent or in a midget impinger containing water, desorption with organic solvents and water, derivatization with 1,3-cyclohexadiene or morpholine, and analysis with gas chromatography using a flame ionization detector. (cdc.gov)
Reaction1
  • This reaction tolerates various substituted arenes, affording para -difluoromethylation products in moderate to good yields. (nature.com)
Spots3
  • Aryanveda Anti Pigmentation Treatment Gel Pen is enriched with actives which prevent liver spots, freckles, sun spot, age spot, and hyperpigmentation due to sun exposure, hormonal disorders or irritation. (ecplaza.net)
  • Zopiclone spots (Rf=0,34) could be easily identified as a white spots under UV light (366 nm, Desaga), its degradation product-as a violet spots (Rf=0,63) and 2-amino-5-chloropyridine-as blue-violet spots (Rf=0,95). (medline.ru)
  • Because it promotes the lightening of pigment spots, tyrosinase inhibition is one of the mechanisms of depigmenting cosmetic products. (bvsalud.org)
Biological activities1
  • Although these natural products did not exhibit antimicrobial activity or cytotoxicity against HeLa cells, their biological activities in other assays have not yet been investigated. (beilstein-journals.org)
Amino acid1
  • This amino acid natural product features a rigid 3-azabicyclo[3.3.1]nonane ring system containing four stereogenic centers within the piperidine ring. (beilstein-journals.org)
Detect1
  • For example, some of the hydrazine derivatives described in this section have been used to detect periodate-oxidized glycoproteins in gels. (thermofisher.com)
Stability1
  • Stability of serum obtained with tubes containing separating gel: 48 hours at 2-8°C (note the data provided by the tube manufacturer). (cdc.gov)
Native1
  • To contribute to the development of antibacterial products from propolis produced by native Brazilian bees, twenty-nine samples of propolis collected from hives in the state of Minas Gerais, Brazil, were screened for in vitro activity against Aeromonas hydrophila, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. (scielo.br)
Activity4
  • The mouse model of cerebral artery ischemia/reperfusion injury was established to test the anticerebral ischemia-reperfusion injury activity of the puerarin derivatives. (hindawi.com)
  • Studies have shown that puerarin reduced cerebral edema in rats with cerebral ischemia-reperfusion injury, removed lipid peroxidation products, enhanced antioxidant capacity, improved antioxidant activity of the brain tissue, and reduced the degree of focal cerebral ischemic injury [ 2 - 4 ]. (hindawi.com)
  • Marine sponges produce a large number of structurally diverse natural products, including many that exhibit biological activity [1-3] . (beilstein-journals.org)
  • Phosphorodiamidate morpholino oligonucleotides are widely used to interrogate gene function in whole organisms, and light-activatable derivatives can reveal spatial and temporal differences in gene activity. (cdc.gov)
Thin-layer1
Journal2
Preparation1
  • The stage of the preparation of the 3,5-bis(trifluoromethyl)benzylidene derivative is described in Acta Chemica Scandinavia, 1973, 27, pages 929-934. (peptidesforresearch.com)
Columns1
Synthetic1
  • The optimized synthetic route includes a new purification method for isolating (−)-7-amino-7,8-dihydrobisabolene in enantiomerically pure form via recrystallization of its benzamide derivative. (beilstein-journals.org)
Content1
  • It is the product of a collaborative effort led by SHEA, the Infectious Diseases Society of America (IDSA), the Association for Professionals in Infection Control and Epidemiology (APIC), the American Hospital Association (AHA), and The Joint Commission, with major contributions from representatives of a number of organizations and societies with content expertise. (cdc.gov)
Effect1
  • The effect of the psoralen derivatives from radix Angelicae dahuricae on retinoid X receptor- α enzymes transcriptional regulation was reported [ 4 ] and this in turn plays key roles in various biological processes including diabetes and obesity. (hindawi.com)
Treatment2
  • The results reported here provide insight into the application and development of puerarin derivatives for the treatment of vascular dementia. (hindawi.com)
  • Anti Pigmentation Treatment Gel Pen Stop. (ecplaza.net)
Natural3
  • Furanocoumarins are well-known natural products that occur in most evolved genera of the Apiaceae family. (hindawi.com)
  • Natural Product Communications , 11 (1), 5-6. (umn.edu)
  • proposed that these natural products could be derived from a common biosynthetic pathway starting from farnesyl pyrophosphate and glycine [5] . (beilstein-journals.org)
Development1
Study3
  • The structure of both the monoacetylated epimers, i.e., 5- O -acetyl-4- C -hydroxymethyl-1,2- O -isopropylidene-α-D-ribo- and xylofuranose obtained by enzymatic acetylation, has been confirmed by an X-ray study on their corresponding 4- C - p -toluenesulfonyloxymethyl derivatives. (beilstein-journals.org)
  • The present study aims to characterize some Iranian wild species of Valerianaceae with respect to their contents of valerenic acid and its derivatives. (scialert.net)
  • STUDY DESIGN: Forty human sound permanent molars which were extracted for periodontal reasons were used in the experimental protocol as under: Group-A (20 teeth):-A 5X4 mm window of the enamel surface was etched with 37% H3PO4 gel for 15 seconds, washed with distilled water and air dried. (who.int)
Identification1
  • This is the first report for the identification and quantification of valerenic acid derivatives in all organs of Valeriana sisymbriifolia , Valeriana alliariifolia and Centranthus longiflorus in comparison with those of commercial Valeriana officinalis . (scialert.net)
Comparison1
  • Structure elucidation was carried-out by means of spectroscopic analysis and comparison with previously isolated sesquiterpene derivatives from the plant. (umn.edu)