Production enzymatic incorporation. Medical search. Frequent questions

Novel processing strategies for hydrolysis and fermentation of lignocellulosic biomass in a single reactor offer large potential cost savings for production of biocommodities and biofuels. (researchgate.net)
Collagen hydrolysate is prepared from collagen or gelatin by chemical or enzymatic hydrolysis under controlled conditions [ 7 ]. (springer.com)
The number of different molecular species in protein hydrolysates (observed with MS) can be high due to (1) the production of hydrolysates by chemical hydrolysis and/or enzymes with broad specificity, (2) the presence of partial modification sites, (3) the (original) presence of non-linear structure elements, and (4) the formation of multiple charge states and/or adduct types during analysis. (springer.com)
In addition, the utility cost of enzymatic hydrolysis for the bioethanol production is much lower as compared to other alternative methods due to an incorporation of fewer treatment steps & working efficiently at mild conditions. (decisiondatabases.com)
Enzymatic hydrolysis of membrane phospholipids by phospholipases produces these lipid second messengers, as well as soluble second messengers such as inositol 1,4,5-trisphosphate, and also other molecules such as choline (Cho) that are released to the extracellular medium. (jneurosci.org)
It is generally assumed that enzymatic hydrolysis of phospholipids leads to phospholipid degradation and membrane damage. (jneurosci.org)
To circumvent this problem, unconventional reactors with different impeller configurations are developed in order to favor mixing and, consequently, enzymatic hydrolysis. (fapesp.br)
In addition to reactor configuration and solids loading, another important factor in the efficiency of enzymatic hydrolysis is the selection of the appropriate enzyme cocktail for the type and pre-treatment of biomass. (fapesp.br)
Currently, the research group of the Fermentation Laboratory I of the Biotechnology Department of the Engineering School of Lorena, University of São Paulo (EEL/USP), is studying the enzymatic hydrolysis of the cellulosic and hemicellulosic fractions of rice straw conditioned to a mild alkaline deacetylation pretreatment that was developed by the group itself. (fapesp.br)
Within this context, the present work proposes to evaluate the effect of xylanase supplementation and the performance of the unconventional reactor with an anchor-type impeller in the enzymatic hydrolysis at high-solids loading of deacetylated rice straw. (fapesp.br)
The production process starts with pretreatment and enzymatic hydrolysis, which generates monomer sugars for fermentation into ethanol. (ualberta.ca)
This work aimed to study the bioethanol production from cardoon hemicellulosic hydrolysates, which originated from dilute sulfuric acid hydrolysis pretreatment. (ua.pt)

We aim to integrate the transformed enzymes within industrial biotechnological processes and to deliver sustainable alternatives for the production of high value chemicals and for the degradation of polymer waste. (nottingham.ac.uk)
With bioavailable NO stored as S-nitrosoglutathione (GSNO), its enzymatic degradation by S-nitrosoglutathione reductase (GSNOR) has a large impact on cellular SNO levels. (uwindsor.ca)
However, the mechanisms by which phospholipases switch from physiological second-messenger production to phospholipid degradation remain unclear. (jneurosci.org)
Enzymatic degradation of excess nucleotides allows the reaction to be performed in a single tube. (cdc.gov)

Lipid mediators, including PAF are produced from membrane phospholipids by enzymatic cascades. (rhea-db.org)

Compared with intact protein, the ingestion of protein hydrolysate accelerates protein digestion and absorption in the gut, increases amino acid availability after passage through the stomach, and tends to increase the incorporation rate of dietary amino acids into skeletal muscle protein [ 2 ]. (springer.com)
Additionally, it is proposed to evaluate the fermentative profile of the yeast Candida guilliermondii FTI 20037 using the obtained enzymatic hydrolysate. (fapesp.br)
Finally, to explore the use of feedstocks that would be more representative of the cellulosic ethanol industry, the enzymatic hydrolysate of wood pulp was fed into the newly established SCF system for ethanol production. (ualberta.ca)
Concluding, the production of bioethanol through detoxification of hemicellulosic cardoon hydrolysate presents a suitable alternative for the production of second-generation bioethanol, especially using the modified E. coli. (ua.pt)

A growing number of studies support the appreciation that LAB can also significantly, positively and negatively, contribute to the sensorial profile of wine through many different enzymatic pathways. (frontiersin.org)
This has led scientists to consider implementing biological routes in their production process by incorporating heterologous or improving inherent esterification pathways. (asu.edu)
Synthetic biology, the incorporation of engineering principles into biology, has given white biotechnology a substantial impetus by enabling the rational design or improvement of production pathways . (plos.org)
Abiotic stresses in plants turn on antioxidative defense machinery which utilizes either of two pathways, i.e . enzymatic or non-enzymatic antioxidants, in an attempt to reduce the stress (Mahmood et al. (biomedcentral.com)

Knowledge of alkaloid biosynthesis and the development of knowledge regarding genome and transcription for lupins are creating opportunities for the production of improved lupin plants. (wikipedia.org)
Pyrrolizidine alkaloids Biosynthesis: importance of putrescine and spermidine as substrates of enzymatic homospermidine formation. (wikipedia.org)
JH biosynthesis involves a series of enzymatic reactions that are conventionally organized into two modules. (nature.com)
Surprisingly, the enzyme catalyzed not only biosynthesis of PAF from lyso-PAF but also incorporation of arachidonoyl-CoA to produce PAF precursor membrane glycerophospholipids (lysophosphatidylcholine acyltransferase activity). (rhea-db.org)
We could show the incorporation of methoxine during biosynthesis by its addition to the culture medium. (biomedcentral.com)

Biofuel enzymes are dominantly used in the advanced biochemical processes for the production of biofuels. (decisiondatabases.com)
White biotechnology takes advantage of enzymatic processes to produce high-value chemicals from sustainable sources with less energy consumption as well as waste production. (plos.org)
In this review, we summarized the processes involved in ROS production in response to several types of abiotic stress in cotton plants. (biomedcentral.com)
Optimization of grape pomace extract (GPE) was performed through the optimization of xylooligosaccharides (XOS) production, using conventional (acid and alkaline extraction) or enzymatic processes. (ucp.pt)

Over the past few years, an increased demand for biofuels has led to the incorporation of enzymes in the production process, in order to enhance the quality of the product and to overcome the shortcomings related to the usage of traditional chemical catalysts as well. (decisiondatabases.com)
Biofuel enzymes have emerged as one of the vital feedstock for the production of biofuels and are projected to witness a substantial demand in the near future due to its various advantages such as non-toxicity, bio-degradability and being nature friendly. (decisiondatabases.com)
Advanced biofuels incorporation into the transportation sector, particularly cellulosic bioethanol, is crucial for attaining carbon neutrality by 2050, contributing to climate changes mitigation and wastes minimization. (ua.pt)

2003), this is the first report-to the best of our knowledge-regarding optimization of agriculture wastes incorporation into soil. (scialert.net)

This project aimed to valorize a major wine production by-product, grape pomace, through the production and characterization of a high valued extract for food industry, improvement of intestinal bioavailability through a gastrointestinal delivery system, and application in the development of a functional beverage as a proof-of-concept. (ucp.pt)

Methoxine incorporation in recombinant proteins is likely to occur when an excess of methanol is present during fermentation but can be avoided when the methanol feed rate protocol is carefully designed. (biomedcentral.com)

Using the appropriate water quality for each step will not only eliminate the risk of water contaminants interfering with nucleic acids and the many enzymatic reactions performed in this process, but will also help prevent instrument contamination. (sigmaaldrich.com)
Water may contain contaminants, albeit in minute amounts, which can directly interfere with DNA and/or with the enzymatic reactions performed during library preparation (e.g., end-repair, ligation, PCR). (sigmaaldrich.com)
A set of coupled enzymatic reactions, together with bioluminescence, detects incorporated nucleotides in the form of light pulses, which produces a profile of characteristic peaks in a pyrogram. (cdc.gov)

Expression of pQexpE increased cellulolysis by ϳ 30% in both the WT and ET strains (Fig. 5A) and boosted ethanol production by 70% relative to the ET strain ( P Ͻ 0.01), thereby restoring ethanol yields to WT levels (Fig. 5B). (researchgate.net)
CO 2 production increased disproportionately relative to H 2 production in WT and ET strains expressing pQexpE (Table 3). (researchgate.net)
The addition of sludge, as a rich source of nitrogen and labile carbon, enhanced the decomposition of straw by the above mentioned actinomycete strains and was suggested to accelerate its incorporation into soil. (scialert.net)
The initial objective of this study was to characterise different available strains of the producer organism, to determine if there was a difference in the production profiles of Trcs by the producer organism. (sun.ac.za)
parabrevis 5618, 362, 10068 and 8185 strains in terms of their production profiles, with the 5618 showing a marked improvement in Trc analogues rich in tryptophan. (sun.ac.za)

In June 2011, Genencor has launched a leading enzyme product to accelerate the cellulosic biofuel production globally. (decisiondatabases.com)
In Oct 2012, Novozymes A/S, a Danish biotech company has launched the enzyme "Avantec", to enhance the biofuel production from the same amount of corn. (decisiondatabases.com)
In Oct 2015, Novozymes has introduced a new enzyme for biofuel production, aimed at better sales growth and market share in the U.S. market. (decisiondatabases.com)
As the similar biochemical catalyzing system with biofuel production, composting was an economic and social adopted method, by which the organic solid wastes could be decomposed and transformed into stable and humus-like substances. (researchsquare.com)
Specifically, this change will force biofuel producers to adopt novel technologies that promote more cost-efficient biofuel production, or that result in co-production of high-value products, transforming ethanol plants into multiple-product biorefineries. (futureenergysystems.ca)

This sequencing technology monitors the incorporation of nucleotides by detecting the protons being released. (sigmaaldrich.com)
A sequencing primer is hybridized to the single-stranded DNA, and incorporation of added nucleotides is detected as light peaks by an enzymatic cascade. (cdc.gov)

As an example of one of the ongoing studies in the lab, the Bressler lab is examining the enzymatic digestion of hardwood kraft pulp to generate a sugar stream for fermentation platforms, as well as a solid residue that is more efficiently converted to cellulose nanocrystals (CNCs), a lightweight and extremely strong nanomaterial that currently sells for $1000 per kilogram. (futureenergysystems.ca)
In addition, the Bressler lab, in collaboration with Prof. Dominic Sauvageau, is examining the incorporation of a self-cycling fermentation strategy for cellulosic ethanol production. (futureenergysystems.ca)
Based on these preliminary results, the Bressler and Sauvageau labs have developed a 5-L self-cycling fermentation system that will be used to optimize ethanol production from a wide variety of feedstocks. (futureenergysystems.ca)
As a result, stable patterns for glucose consumption and ethanol production were observed for SCF after cycle 1, but using only about 1/3 of the fermentation time of batch fermentation. (ualberta.ca)
Taken together, this work demonstrated that an advanced cycling fermentation strategy can significantly improve ethanol productivity, which will contribute to a reduction of production cost for the cellulosic ethanol industry, thus helping to overcome energy and environmental challenges. (ualberta.ca)

It was found that there was a ~7.5X decrease in ethyl acetate production in the cell media versus synthetic samples with the same concentration of reactants. (asu.edu)
Such a large decrease indicates that enzymatic promiscuity or inhibition currently prevent the cell samples from reaching the same conversion as synthetic studies. (asu.edu)
Comprehensive chirality Synthetic methods VI: enzymatic and semi-enzymatic. (nottingham.ac.uk)
With the incorporation of the gas flow meter, an SCF system using synthetic medium was successfully operated for ethanol production in a 5-L fermenter. (ualberta.ca)

therefore, plants have evolved a complex array of enzymatic and non-enzymatic detoxification mechanisms to combat oxidative damage due to ROS. (biomedcentral.com)

With a two second HDX reaction, the residues Gly321, Lys323, and Lys188 displayed a decrease in deuterium uptake of 1.4%, 1.4%, and 0.4%, after the incorporation of GSNO. (uwindsor.ca)

Recent data have provided bench-scale proof-of-concept that a cycling strategy could be applied to cellulosic ethanol production, resulting in increased productivity (amount of ethanol produced per year). (futureenergysystems.ca)
Despite the extensive technological developments achieved over the years for cellulosic ethanol production, the current industry is still faced with economic challenges, hindering its rapid expansion. (ualberta.ca)

This supports the hypothesis that glucose aids to regenerate active copper in the catalytic cycle of NO production. (uwindsor.ca)
From insulin production in genetically modified bacteria to the synthesis of artemisinic acid - a malaria drug precursor - in engineered yeast, complex and sought-after chemicals can be produced essentially from glucose as a starting material. (plos.org)

One critical challenge is retaining high enzyme production in the presence of elevated product titers. (researchgate.net)
This technique has been widely used in applied sciences such as optimizing enzyme production and assessment of drug interactions (Kalil et al . (scialert.net)

Oxidative stress occurs when crop plants are exposed to extreme abiotic conditions that lead to the excessive production and accumulation of reactive oxygen species (ROS). (biomedcentral.com)
Plants have evolved defense mechanisms for the production of antioxidants to detoxify the ROS and to protect the plant against oxidative damage. (biomedcentral.com)
When ROS production is greater than a plant's ability to scavenge excess ROS, there is rapid leakage of excess ROS (a state known as oxidative stress) into other parts of the plant. (biomedcentral.com)
Increased oxidative stress due to high production of reactive oxygen species (ROS) is proposed to be involved in pathogenesis of SS 5 . (bvsalud.org)

Further, we focus on recent advancements in the production of resistant cotton varieties by conventional and transgenic incorporation of defense genes against several abiotic stresses. (biomedcentral.com)
Despite this significant production capacity, the benchtop scale of Touchlight's proprietary dbDNA™ ("doggybone DNA") manufacturing technology enables a total facility footprint of just 7,500 sq ft, a fraction of the space required by conventional bioreactor-based plasmid DNA manufacture. (asgct.org)

To augment ethanol production by the ET strain, an alternative ethanol production pathway comprised of pyruvate decarboxylase (Pdc) and alcohol dehydrogenase (AdhB) from Zymomonas mobilis (Fig. 4A) was transferred into C. phytofermentans on the replicating pQexpE plasmid (Fig. 4B). (researchgate.net)
Despite the integration of an SCF operation strategy into many microbial cultivation systems, it has not yet been successfully applied to ethanol production. (ualberta.ca)
To apply a real SCF strategy into ethanol production, the process needs to be automatically monitored and driven by a feedback control parameter. (ualberta.ca)

In particular, we have developed methods for the stereoselective incorporation of the 1,2,4-dithiazolidine-3,5-dione (Dts imide) moiety into molecules as a masked isocyanate equivalent. (exeter.ac.uk)

We cater for the chemical and biotech industries, as well the agri-food and health sectors, with a view to: validating an enzymatic assay method, improving the physico-chemical and rheological properties of an industrial product, and creating a functioning extraction process. (capacites.fr)
Although these adjuncts present lower production cost adjuncts materials can affect the physical-chemical and sensory properties of the beer [ 8 8 Bogdan P, Kordialik-Bogacka E. Alternatives do malt in brewing. (scielo.br)

Our results showed that, for production of ethyl acetate through this reactive extraction approach, Novozyme435 exhibited significant esterification with chloroform, with almost 85% conversion efficiency. (asu.edu)
By optimizing the reactive extraction process for ester production, biorefineries could become more competitive and economically feasible for numerous applications. (asu.edu)

Touchlight's revolutionary enzymatic production platform enables unprecedented speed, scale, and the ability to target genes with a size and complexity that is impossible with current technologies. (asgct.org)
Knockout of relevant genes from the known DMSP demethylation pathway to MeSH showed in both species a residual production of [ 2 H 3 ]MeSH, suggesting that a second demethylation pathway is active. (beilstein-journals.org)

In case of accidental soil incorporation in ensiled material, a long aerobic phase or slow acidification, the microbial communities in the silage will be dominated by Clostridia, yeasts, molds and accidentally incorporated pathogenic microorganisms such as Listeria sp. (benisonmedia.com)

This thesis consists of two components: chapter one explores the production of NO generating particles, that use chitosan as a matrix for a copper catalyst. (uwindsor.ca)
These particles were successfully tested in their release of authentic NO: the maximum rate of production was 1.40 nmol/min/g. (uwindsor.ca)
Sensory analysis allowed to conclude that the incorporation of particles did not promoted significant differences in most of evaluated attributes. (ucp.pt)

In addition to strain-determined differences in production profiles, differences in production as a result of altered nutrient conditions, in the form of nitrogen, sulphate and carbon sources, added on top of a base media, were also investigated. (sun.ac.za)
Urea appeared to have the most marked effect on Trc production, particularly for the 5618 strain. (sun.ac.za)
Following this, small scale cultures were done under selected conditions to test for their incorporation using the 5618 strain. (sun.ac.za)
Methoxine is likely synthesized by the enzymatic action of O -acetyl homoserine sulfhydrylase and we confirmed that Nanobodies produced in the corresponding knock-out strain contained no methoxine variants. (biomedcentral.com)

In principle, the medium containing the precursors will be treated with the enzyme in presence of organic solvent, where the precursors will be distributed in both the phases, aqueous and organic, based on their polarity, and the enzymatic esterification will happen at the interface. (asu.edu)
Enzymatic activity may be affected by traces of organic molecules (humic acids), and ions or metals (magnesium, iron, etc.) that are potentially present in water. (sigmaaldrich.com)
In this area, we have developed a methodology for the incorporation of protected isocyanates into organic molecules and this chemistry is being applied to the synthesis of a diverse set of biologically important, nitrogen-containing heterocycles. (exeter.ac.uk)

In contrast, NMDA strongly reduced the incorporation of [ 3 H]choline and [ 3 H]ethanolamine into their respective phospholipids. (jneurosci.org)

Enzyme cocktails can alter the lignin and hemicellulose content in wood cell walls, improving the bleaching process during pulp production and offsetting the need for toxic chemicals. (ncsu.edu)
Commercial β-glucanase application in malt allowed accelerating the malting process without affecting the quality of the malt for beer production. (scielo.br)
Low yield and lack of homogeneity will impact the economics of the production process and hence, the costs for the therapeutic overall. (biomedcentral.com)
Enzymatic process extracted up to 88.7 ± 0.12% of total. (ucp.pt)

This new approach has been shown to preserve mobility and enzymatic activity of transmembrane proteins in the resulting nSLB. (lu.se)

In recent times, whole organisms ranging from bacteria over yeasts up to plants are used for production by enzymatic catalysis. (plos.org)

Using a redox lipidomics approach, we have identified oxidatively-truncated PE species (trPEox) in enzymatic and non-enzymatic model systems. (cdc.gov)

If successful, this could allow storage of renewable electricity through electrochemical or enzymatic fixation of carbon dioxide and subsequent storage as carbon-based energy storage molecules including hydrocarbons and non-volatile polymers at high efficiency. (biomedcentral.com)

Aim: To evaluate the effect of astaxanthin, fish oil and association of them on enzymatic antioxidant system and functional parameters of salivary glands. (bvsalud.org)

At first step of ensilingisthe enzymatic activity of intact plant cells whenresidual respiration occurs. (benisonmedia.com)

This was initially done by growth rate analysis of the producer organisms under different conditions, and then followed up by small scale culture to determine the effect on production of Trcs. (sun.ac.za)

In the dipteran Drosophila melanogaster , TGFbeta signaling stimulates JH production by up-regulating the transcription of the JH acid methyltransferase (JHAMT ) gene through the transcription factor Mothers against Dpp (Mad) 14 . (nature.com)
This arises due to the non-ribosomal, enzymatic, synthesis of these peptides in which certain domains within this multi-domain enzyme system have the ability to incorporate more than one kind of aromatic amino acid. (sun.ac.za)

In recent years the methylotrophic yeast Pichia pastoris has received wide acceptance for the production of biopharmaceuticals [ 3 ]. (biomedcentral.com)

For that reason, red wine is richer in resveratrol than white wine, because during the production of red wine, parts of the grape where resveratrol is concentrated are macerated. (hindawi.com)

The alternative production strategy has drawn more attention due to the increasing consciousness about the social sustainability, which lead to the development of renewable fuels. (researchsquare.com)

Anatomy21

Organisms9

Diseases1

Chemicals and Drugs150

Analytical, Diagnostic and Therapeutic Techniques and Equipment24

Phenomena and Processes49

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science3

Health Care1

Hydrolysis12

Degradation4

Membrane phospholipids1

Hydrolysate4

Pathways4

Biosynthesis5

Processes4

Biofuels3

Optimization1

Characterization1

Recombinant proteins1

Reactions3

Strains5

Biofuel production5

Nucleotides2

Fermentation5

Synthetic4

Detoxification1

Deuterium1

Cellulosic ethanol production2

Glucose2

Enzyme production2

Oxidative4

Conventional2

Ethanol3

Methods1

Chemical2

Extraction2

Genes2

Soil1

Particles3

Strain4

Organic3

Strongly1

Process4

Preserve1

Bacteria1

Species1

Carbon1

Functional1

Activity1

Conditions1

Acid2

Yeast1

Grape1

Alternative1