Producing heterologous antibodies. Medical search. Frequent questions

Cats with blood group A, however, have only low anti-B antibody titers (Fig. 1). (laboklin.com)
Studies determining the antibody titers of anti-A in B queens have shown that even low titers can cause NI. (laboklin.com)
Hapten-heterologous carrier conjugates elicited antihapten titers similar in magnitude to those elicited by the homologous carrier conjugate. (caltech.edu)
Partial suppression was manifested in decreased frequency and delayed appearance of the response as well as decreased maximal antibody titers. (caltech.edu)
the homologous and heterologous serological titers of a specific serum can be integrated in a bidimensional system which characterizes the criteria of relationship and dominace. (liverpool.ac.uk)
Env trimer-binding antibodies (Abs) were readily induced after the second immunization, showing increasing titers and durability after each booster injection. (natap.org)
Following the third heterologous boost, cross-neutralizing Abs against tier-2 viruses of different clades started to appear in all animals, reaching higher and more stable titers after the last immunization. (natap.org)
In contrast, viremia was below the limit of detection in pups given birth to to PDK53-immunized dams (Number 1C), therefore indicating safety by maternal antibodies and correlating with the strong PRNT50 titers measured against strain 16681 in these 3-wko pups (Number 1B). (siamtech.net)
In both adult mice vaccinated with PDK53 and pups given birth to to PDK53-immunized dams, PRNT50 titers against the heterologous D2Y98P-PP1 strain (Number 2, A and B, and Supplemental Number 1, A and B) were. (siamtech.net)
Our results demonstrated that the Y-5A15 remarkably enhanced the Aβ epitope immunogenicity and elicited high antibody titers against Aβ in AD mice. (mdpi.com)
In the first phase test of the two vaccines, high titers of neutralizing antibodies can be induced, and strong CD4+ and CD8+ reactions are produced, accompanied by mild to moderate adverse reactions. (medicaltrend.org)

The yeast Pichia pastoris (also known as Komagataella pastoris ) has been used for over 30 years to produce thousands of valuable, heterologous proteins, such as insulin to treat diabetes and antibodies to prevent migraine headaches. (bepress.com)
Despite its success, there are some common, stubborn problems encountered by research scientists when they try to use the yeast to produce their recombinant proteins. (bepress.com)
RESULTS: We chose endogenous secretory enzymes and heterologous camelid small antibodies (nanobody) as model proteins. (bvsalud.org)
Monoclonal antibodies (mAbs) are highly specific antigen binding proteins that are used as biological reagents, therapeutics, and in rapid diagnostics. (gatech.edu)
Newly produced antibody, like the other plasma proteins, became readily labelled with S35 amino acids within a few hours. (caltech.edu)
Some success has been obtained also in the formation of antibodies from other serum proteins and by other denaturation-renaturation procedures. (caltech.edu)
In the past decade, yeast have been frequently employed to study the molecular mechanisms of human neurodegenerative diseases, generally by means of heterologous expression of genes encoding the relevant hallmark proteins. (microbialcell.com)
Bispecific antibodies are proteins capable of simultaneously binding two different epitops, on the same or on different antigenes (Figure 2 ). (ens-lyon.fr)
Proteins selected from antibody libraries displayed on phage particles are already in clinical use (Humira®) and many more are undergoing clinical trials for use in the treatment of e.g. cancer, infectious disease and autoimmune disease. (lth.se)
Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL and Curtiss R III (1997) Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. (lth.se)
Overproduction of Hsp70 family members members with periplasmic proteins Hsp70 co manufacturing has also been employed to benefi cial effect with heterologous proteins generated within the E. coli periplasm, apparently by growing the solubility with the protein precursor prior to membrane translocation. (screeninglibraries.com)
This study evaluated the minimum requirements for the formation of phi6-specific vesicles and the possibility to localize P9-tagged heterologous proteins into such structures in Escherichia coli . (biomedcentral.com)
A P9-GFP fusion protein was used to study the targeting of heterologous proteins into P9 vesicles. (biomedcentral.com)
The possibility to locate heterologous proteins into the P9-lipid vesicles facilitates the production of vesicular structures with novel properties. (biomedcentral.com)

A much more promising strategy is to use recombinant monoclonal antibodies (mAbs) against influenza and several are currently in clinical development ( 9 - 13 ). (frontiersin.org)
Monoclonal antibodies (mAbs) have great potential as biodefense agents, whether in passive immunization, detection or disinfection. (grantome.com)
In response to the need for a rapid and inexpensive system for the large scale production of mAbs, we have begun to develop eukaryotic algae as a production platform for the expression of human antibodies. (grantome.com)
Here, we use a method of mAb production in which we pair conjugate virus-like particle (VLP) vaccine with hybridoma technology to produce high-affinity mAbs against three classes of molecules 1) fentanyl derivatives, 2) SARS-CoV-2 peptides, and 3) α-amanitin and microcystin LR cyclic peptide toxins. (gatech.edu)
We successfully produced broad and derivative-selective mAbs against eight fentanyl derivatives. (gatech.edu)
Lastly, we produced high affinity mAbs for both α-amanitin and microcystin LR, two highly toxic cyclic peptides. (gatech.edu)
The early success of mAb production against the variety of targets presented in this thesis shows the viability and exceptional versatility of conjugate VLP vaccines as a means to producing mAbs. (gatech.edu)
Monoclonal antibodies (mAbs) have been very successful treatments for a wide range of diseases, particularly cancers and autoimmune disorders. (pharmasalmanac.com)
Long-term delivery of anti-HIV monoclonal antibodies (mAbs) using adeno-associated virus (AAV) vectors holds promise for the prevention and treatment of HIV infection. (natap.org)
Our results provide proof of concept for AAV-delivered mAbs to produce a "functional cure. (natap.org)
By single B-cell cloning and antibody amplification from a protected macaque, we derived a panel of mAbs that share genetic similarities with previously identified macaque and human bNAbs against the CD4-BS. (natap.org)
Neutralizing monoclonal antibodies (MAbs), particularly those having cross-clade neutralizing activity, play a critical role in immunoprotection against various influenza A virus (IAV) infections, particularly those caused by the highly pathogenic avian influenza H5N1 virus and any future unpredictable virus strains. (justia.com)
Neutralizing antibodies can provide a first line of defense against influenza pathogens and passive immunization with neutralizing MAbs can provide immediate effects to prevent the spread of influenza infection and mortality. (justia.com)
Disclosed herein are neutralizing monoclonal antibodies (MAbs) specific for the surface hemagglutinin (HA) protein of the influenza H5N1 strain. (justia.com)
Monoclonal antibodies (mAbs) have significantly advanced the treatment of a variety of human diseases, particularly the ability to treat cancer. (ens-lyon.fr)

This report aims to understand the persistence of immune response to the viral vectored, mRNA and protein-based COVID-19 vaccine platforms used in homologous and heterologous priming combinations, which will inform the choice of vaccine platform in future vaccine development. (jenner.ac.uk)
Schistosoma mansoni antigens in the early life alter homologous and heterologous immunity during postnatal infections. (fiocruz.br)

The vaccine is a self-assembling, germline-targeting HIV nanoparticle designed to prime a type of B cells that produce precursors to broadly neutralizing antibodies that target a protein on the surface of the virus. (scienceboard.net)
McElrath called the data a first step, adding that heterologous booster vaccines will still be needed to eventually produce VRC01-class broadly neutralizing antibodies. (scienceboard.net)
Despite intensive research over the past four decades, an HIV-1 vaccine capable of inducing broadly neutralizing antibodies (bNAbs) and protection from heterologous tier-2 strains is still wanting. (natap.org)

Collectively, the findings show that the vaccine acts on the immune system in a way that may improve human immune responses, either to heterologous boost immunogens after the prime vaccination, or to other human vaccine immunogens. (scienceboard.net)
We investigated safety, tolerability, and immunogenicity of the heterologous 2-dose Ebola vaccination regimen in healthy and HIV-infected adults with different intervals between Ebola vaccinations. (plos.org)
Antibody levels persisted to at least 1 year, and Ad26.ZEBOV booster vaccination demonstrated the presence of vaccination-induced immune memory. (plos.org)
Dengue viruses are members of the Flaviviridae and have sufficient antigenic similarity to yellow fever virus, Japanese encephalitis virus, and West Nile virus that previous infection or vaccination may raise cross-reactive serum antibodies. (cdc.gov)
Persistence of immune response in heterologous COVID vaccination schedules in the Com-COV2 study - a single-blind, randomised trial incorporating mRNA, viral-vector and protein-adjuvant vaccines. (jenner.ac.uk)
In a new study, she and her colleagues found that intranasal vaccination provides broad-based protection against heterologous respiratory viruses in mice, while so-called systemic immunization, which uses an injection to elicit body-wide protection, did not. (eurasiareview.com)
Furthermore, pups failed to seroconvert after PDK53 vaccination, owing to maternal antibody interference. (siamtech.net)
Although vaccination is an important strategy to prevent influenza infection, most of the current vaccines cannot provide immediate protection in the event of influenza pandemics and epidemics due to the length of time required for producing effective vaccines. (justia.com)
This study was performed to test the hypothesis that immunity to heterologous vaccination would improve when the parasites were removed. (uky.edu)
The effect of gastrointestinal parasites on heterologous vaccination has not been addressed in equids. (uky.edu)

Immunisation with BNT/NVX generated a qualitatively different antibody response to BNT/BNT, with the total IgG significantly lower than BNT/BNT during all follow-up time points, but similar levels of neutralising antibodies. (jenner.ac.uk)

Although this microorganism has high potential for protein production, it has not yet been widely used for heterologous recombinant protein production. (bvsalud.org)
To overcome this challenge, we first used an inducer-free biomass-degrading enzyme expression system, previously developed to produce cellulases and hemicellulases using glucose as the sole carbon source, for recombinant protein production using T. reesei. (bvsalud.org)
This system would be a novel platform for heterologous recombinant protein production in T. reesei. (bvsalud.org)
Successful completion of this study should provide the proof of concept for using algae as a rapid means to produce large quantities of recombinant human antibodies. (grantome.com)
In two independent antibody purification/depletion experiments with permutated order of VAR2CSA variants, IgG purified on the first VAR2CSA antigen displayed broad cross-reactivity to both recombinant and native VAR2CSA variants, and inhibited binding of all isolates to CSA. (elifesciences.org)

Unlike vaccines which elicit a system-wide immune response, IgA antibodies work locally on mucosal surfaces found in the nose, stomach, and lungs. (eurasiareview.com)
Nasal vaccines, but not the shot, also induced antibodies that protected the animals against a variety of flu strains, not just against the strain the vaccine was meant to protect against. (eurasiareview.com)
While both vaccine injections and nasal vaccines increased levels of antibodies in the blood of mice, only the nasal vaccine enabled IgA secretion into the lungs, where respiratory viruses need to lodge to infect the host, Iwasaki said. (eurasiareview.com)
In addition, we are investigating antibody responses to SARS-CoV-2 and related zoonotic coronaviruses (CoV), with the related goal of developing broadly-protective immunotherapies and vaccines against variants of concern and emerging CoV threats. (stanford.edu)
Furthermore, these vaccines are limited to one or just a few strains and don't produce highly potent neutralizing antibodies or cross-reactive immunity against divergent influenza viruses. (justia.com)
However, the first VAR2CSA subunit vaccines failed to induce broadly neutralizing antibody and it is known that naturally acquired antibodies target both variant-specific and conserved epitopes. (elifesciences.org)
In contrast, first-generation vaccines based on VAR2CSA fragments have only induced variant-specific antibodies, therefore offering limited protection against infection. (elifesciences.org)
Although the vaccines produced by Pfizer and Moderna have proven to have good efficacy and safety, their demand for cold chain storage has brought huge difficulties to guarantee. (medicaltrend.org)
1. Rabies vaccines induce an active immune response that includes the production of neutralizing antibodies. (cdc.gov)

There was no correlation between anti-TNP-precipitating antibody titer after sensitization and the ability to respond to challenge by hapten-heterologous carrier. (caltech.edu)
Quantitative antibody nitrogen data, obtained for the serum of each rabbit showed, in general, an inverse relationship between circulating antibody and radioactivity retained, i.e. the higher the circulating antibody titer, the lower the retention of radioactivity in liver tissue. (caltech.edu)
Many clinical studies on AD immunotherapies have failed due to low safety and efficacy, calling for a highly potent AD vaccine which induces sufficient antibody titer while avoiding side effects. (mdpi.com)
Spike-specific binding antibodies (bAbs) in all participants and neutralizing antibodies (nAbs) against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) wild-type, Delta, and Omicron variants in CPs were analyzed and presented as the geometric mean titer. (bvsalud.org)

After challenge with heterologous tier-2 SHIV (AD8) by 12 repeated low-dose rectal inoculations, significant protection was observed in a group of animals, with no difference between those immunized with mRNA vs. mRNA+protein. (natap.org)
Altogether, the data indicated that pups given birth to to PDK53-dams were safeguarded from homologous 16681 challenge but experienced ADE upon challenge with heterologous DENV2 strain D2Y98P-PP1. (siamtech.net)

A sensitive, competitive indirect heterologous enzyme-linked immunosorbent assay (ELISA) was developed. (cdc.gov)
We screened an age-stratified collection of serum samples from 535 humans in Italy, using virus-like particles of genotypes GIV.1, circulating in humans, and GIV.2, identified in animals, in ELISA, in order to investigate the prevalence of GIV NoV-specific IgG antibodies. (cdc.gov)
After a primary infection with a heterologous flavivirus, subsequent antibody testing by ELISA may produce false positive results for a different flavivirus. (cdc.gov)
Many commercially available antibodies are suitable for traditional ELISA assays or western blotting but lack sensitivity and specificity in multiplex format. (bioradiations.com)
Antibody levels determined by ELISA showed that animals with low levels of parasites had a trend toward increased KLH specific total immunoglobulin, IgG(T) and IgA compared to heavily parasitised ponies. (uky.edu)

The presence of dinitrophenyl groups on the immunizing antigen could suppress, partially or completely, the antibody response to p-azophenyl arsonate when this hapten was located on the same molecule. (caltech.edu)
These findings appear irreconcilable with the possibility of direct clonal selection of antibody-producing cells by unprocessed antigen. (caltech.edu)
The purpose of the present investigation was to study further, such antibody which appeared to be bound with antigen and perhaps normal tissue constituents. (caltech.edu)
After varying lengths of time, when circulating antibody had declined, antigen was again injected. (caltech.edu)
The specific activity of antibody was measured in the antigen-antibody precipitates obtained, both in the period when the antibody had been allowed to decline and also after antigen had been reinjected to induce an anamnestic response. (caltech.edu)
The biological activity of soluble antigen-antibody complexes: II. (caltech.edu)
2), and ourselves (3) has established the fact that soluble antigen-antibody complexes formed in excess antigen can, (a) induce symptoms similar to anaphylaxis, (b) cause contraction of isolated smooth muscle from normal guinea pigs, and (c) increase the permeability of skin capillaries in a manner similar to that obtained in passive cutaneous anaphylaxis. (caltech.edu)
Passively administered homologous antibody did not produce a change in the retention of the primary injection of antigen nor did secondary injections of a heterologous native protein injected according to the same immunization schedule as the homologous azoprotein. (caltech.edu)
From these results it may be concluded that an intracellular antibody-forming activity influences the loss (or retention) of antigen deposited in liver tissue and that the mechanism is immunologically specific. (caltech.edu)
1) The general approach has been to attempt to produce an insoluble protein antigen which would combine specifically with antibody to give a complex that could be dissociated into soluble antibody and insoluble antigen, which could then be separated by centrifugation. (caltech.edu)
However, in every case the resulting product either had lost too much native antigenicity (i.e., adsorbed serum always contained antibody which reacted with native antigen), was too soluble, or had too much power of adsorption for non-specific protein. (caltech.edu)
We evaluated hypersensitivity reactions (HR), antibodies levels (IgG1/IgG2a) anti-soluble egg antigen and anti-soluble worm antigen preparation, and anti-OA, cytokine production, and CD4+FoxP3+T-cells by splenocytes. (fiocruz.br)
Currently, most of the antibodies for therapeutic usage are monospecific, with a defined specificity for a particular molecular part of an antigen, known as an epitope. (ens-lyon.fr)
Proteolytic enzyme produced by group A streptococci with special reference to its effect on type-specific M-antigen. (microbiologyresearch.org)

We also showed early signs of success targeting neutralizing and mutant SARS-CoV-2 peptides with conformational specificity using a heterologous prime-boost strategy. (gatech.edu)
Bispecific (bsAbs) and trispecific antibodies overcome this issue by providing two or three binding sites, respectively, increasing specificity and efficacy while reducing side effects. (pharmasalmanac.com)
The specificity of tissue antigens has previously been investigated by the usual immunologic procedures [1] and the effects of antibodies [2,3] upon tissue explants has long been known. (caltech.edu)
The growth and function of tissue explants have previously been used to study the specificity of tissue antibodies [4,5], but the antisera used were of low, uncertain potency, and only a few different tissues were examined in testing the toxic effects. (caltech.edu)
Since the development and validation of multiplex assays are very complex and time consuming, we've used our vast expertise to develop novel approaches to address multiplex cross-talk, optimize reagents to improve sensitivity, apply a clinical-like approach to improve reproducibility, and develop antibodies and antigens for greater specificity. (bioradiations.com)

CD4 T-cell help will be needed to develop the high-affinity neutralizing antibody responses that the researchers aim to induce to protect against HIV. (scienceboard.net)

[ 6 ] In 1983, Karmali and colleagues reported finding a toxin produced by specific strains of Escherichia coli in the stools of children with HUS. (medscape.com)
E coli strains produce two types of Shiga toxins. (medscape.com)
The capacity of certain E coli strains to produce Shiga toxins appears to have resulted from transduction of the responsible genes by bacteriophages. (medscape.com)
In earlier studies, such antibodies have shown the ability to neutralize 90% of HIV strains. (scienceboard.net)
Whole cultures and culture filtrates of albus strains and formolized fitrates of aureus strains were found to stimulate the production of anti-lipolytic antibody in rabbit serum. (microbiologyresearch.org)
These findings demonstrate that a single VAR2CSA ectodomain variant displays conserved epitopes that are targeted by neutralizing (or binding-inhibitory) antibodies shared by multiple parasite strains, including maternal isolates. (elifesciences.org)

Antibody responses were observed until Day 365. (plos.org)
The third dose produced increased immune responses comparable to or exceeding levels associated with protection in Phase 3 clinical trials. (novavax.com)
Heterologous ChAd-primed schedules produced and maintained the largest T-cell responses until D196. (jenner.ac.uk)
Anamnestic antihapten responses were obtained to trinitrophenyl (TNP) when rabbits sensitized to trinitrophenyl-hemocyanin (TNP-KLH) were challenged with TNP-heterologous protein conjugates. (caltech.edu)
Although anti-drug antibody (ADA) responses restricted mAb delivery, one monkey successfully maintained 50-150 μg/mL of 3BNC117 and 10-1074 for over 2 years. (natap.org)

The most commonly used systems for monoclonal antibody production rely on transformed Chinese Hamster Ovary cells (CHO) grown in suspension culture. (grantome.com)
In another embodiment, the neutralizing antibody is a monoclonal antibody such as a mouse antibody, a humanized antibody, a chimeric antibody, or a fragment thereof. (justia.com)

1 As of March 2022, 180 bispecific antibodies were in preclinical development, and more than 50 were being investigated in clinical trials. (pharmasalmanac.com)
Progress in antibody engineering has led to the generation of many different types of antibodies that differ in size and shape, including bispecific antibodies. (ens-lyon.fr)
Currently, two large classes of bispecific antibodies have been developed: immunoglobulin-like and small bispecific antibodies. (ens-lyon.fr)
This review summarizes the various methods used to generate bispecific antibodies and discusses the perspectives and the therapeutic benefits of bispecific antibodies. (ens-lyon.fr)
The need to improve their clinical efficacy has led to an increased interest in bispecific antibodies (bsAbs). (ens-lyon.fr)

The problem of obtaining from precipitation experiments evidence about the structure of antibodies and the nature of serological reactions is obviously greatly simplified by the replacement of protein antigens by simple substances of known structure. (caltech.edu)
or (f) living vectored cells expressing specific heterologous antigens. (who.int)

de Haard HJ, van Neer N, Reurs A, Hufton SE, Roovers RC, Henderikx P, de Bruïne AP, Arends JW and Hoogenboom HR (1999) A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies. (lth.se)

Provided are a B cell-derived iPS cell generated using a convenient technique, a technology for providing a human antibody at low cost using the iPS cell, an immunologically humanized mouse prepared using cells differentiated from the iPS cell, and the like. (justia.com)
2) Assemble a Fab and full-length human antibody in algal chloroplast. (grantome.com)

Passive transfer of serum antibodies from convalescent patients has been used in the past ( 7 , 8 ), however, this approach is of limited use in a global pandemic emergency. (frontiersin.org)
PRNT can often resolve cross-reactive serum antibodies in this situation and identify the infecting virus. (cdc.gov)

However, high-titered cross-reactive antibody levels produced from multiple previous flavivirus infections cannot be resolved by PRNT. (cdc.gov)

a heterologous booster is a different brand than the initial vaccine. (harvard.edu)
In the COV-BOOST trial, Nuvaxovid induced a significant antibody response when used as a heterologous third booster dose. (novavax.com)

Specifically we propose to: 1) Optimize heterologous protein expression in alga chloroplast. (grantome.com)
In this way, the genes encoding the protein product will be found inside the virus particle while the function encoded for by the gene (such as the binding properties of an antibody) will be displayed on the surface of the virus. (lth.se)
Most SARS-CoV-2 vaccine candidates produce an immune response to the spike protein on the surface of the virus. (medicaltrend.org)

5) Scale up for production and purification of algal expressed antibodies. (grantome.com)
For the past several years we have been interested in devising methods that would afford practical procedures for the isolation and purification of antibodies from immune serums and in particular the isolation of non-precipitating antibodies from animal serums and allergic antibodies (reagins) from human serums. (caltech.edu)
Antibodies prepared by positive affinity purification and fractionation by ion exchange chromatography are the best options. (bioradiations.com)
These affinity-purified antibodies, which lack unwanted anti-species cross-reactivity, are markedly superior to those obtained with standard purification methods. (bioradiations.com)

We predict that ABCs could be playing a role throughout γHV68 infection due to their long-term persistence, activation of T cells, and continuous cytokine and antibody production. (nature.com)
If the antibodies later encounter the same infection, they help prevent illness by recognizing the microbe and preventing it from entering cells. (harvard.edu)
Analyses of antibody and cellular assays were performed on an intention-to-treat population without evidence of COVID-19 infection at baseline or for the trial duration. (jenner.ac.uk)
Over the course of the COVID-19 pandemic, he has made significant contributions to our understanding of antibody-spike interactions through in-depth structural analysis that detail the specificities and mechanisms of how monoclonal neutralizing antibodies bind spike to prevent infection. (stanford.edu)
The present application is drawn to neutralizing monoclonal antibodies for preventing and treating influenza virus infection and methods of treating influenza virus infection. (justia.com)
Also disclosed herein is a method of treating influenza virus infection in a subject in need thereof comprising administering a therapeutically effective amount of the neutralizing antibody specific for an epitope having at least 90% homology to amino acids +72-115 of the HA1 domain of H5N1 influenza virus hemagglutinin and thereby treating said influenza virus infection in said subject. (justia.com)
In a primary infection, no type-specific immunoglobulin G (IgG) antibodies to either HSV-1 or HSV-2 exist at the time of the outbreak. (medscape.com)
Antibody response occurs 3-4 weeks after the infection and is lifelong. (medscape.com)
A nonprimary first-episode infection is a first genital HSV outbreak in a woman who has heterologous HSV antibodies. (medscape.com)

We are particularly interested in the co-evolution of HIV-1 and broadly-neutralizing IgG antibodies (bNAbs), which may hold the key to the development of an effective HIV-1 vaccine. (stanford.edu)

We have previously described R1a-B6, an alpaca-derived single domain antibody (nanobody), that is capable of potent cross-subtype neutralization in vitro of H1N1, H5N1, H2N2, and H9N2 influenza viruses, through binding to a highly conserved epitope in the influenza hemagglutinin stem region. (frontiersin.org)

The results are discussed in terms of immunogenicity of sensitization, suppressive effects of persisting postsensitization antibody, and submolecular haptenic environment as factors possibly affecting the heterologous recall process. (caltech.edu)

Research in our lab is aimed at defining the structural correlates of broad and potent antibody-mediated neutralization of viruses. (stanford.edu)
The purpose of this assignment was to assess our selected article--Isolation of Potent SARS-CoV-2 Neutralizing Antibodies and Protection from Disease in a Small Animal Model--in terms of accuracy, availability, and reliability. (openwetware.org)
Isolation of potent SARS-CoV-2 neutralizing antibodies and protection from disease in a small animal model. (openwetware.org)

a substance displayed on the surface of a microbe that stimulates the body to produce an immune response. (harvard.edu)
While the protective role of IgA-producing cells had been well established in combatting intestinal pathogens, Iwasaki's lab wondered if triggering IgA response might also produce a localized immune response against respiratory viruses. (eurasiareview.com)

These results highlight the potential of this HIV-1 nanoparticle vaccine approach to induce the critical T-cell help needed for maturing antibodies toward the pathway of broadly neutralizing against HIV," McElrath said. (scienceboard.net)
Therefore, our work advocates for the development of dengue vaccine candidates that induce protecting CD8+ T cells despite the presence of enhancing, interfering maternal antibodies. (siamtech.net)

When challenged on the same day with NNP.F γ G, the two groups of mice undergoing a graft-versus-host reaction (GVHR) showed higher numbers of antibody-producing cells to both NNP and F γ G determinants. (karger.com)
The antibody-forming cells were of host origin, and the effect could be induced by T lymphocytes, not by B. When mice were challenged with NNP.F γ G at various intervals after the induction of GVHR, it was found that by 8 days the increased response was seen only in mice primed to hapten on the heterologous carrier (NNP.OV). (karger.com)
Increased antibody formation was not seen in primed CBA mice injected with (CBA × C57B1)F 1 spleen cells, or in normal (CBA × C57B1)F 1 mice injected with primed CBA cells. (karger.com)
When challenged, these barrier tissues produce B cells which in turn secrete immunoglobin A (IgA) antibodies. (eurasiareview.com)
Cells from these ponies also produced significantly lower levels of IL-4 compared to lightly parasitised ponies. (uky.edu)
Having been exposed to the parasite in prior pregnancies generates antibodies that target VAR2CSA, stopping the infected blood cells from latching onto placental CSA or tagging them for immune destruction. (elifesciences.org)

It is estimated that bsAbs account for close to 20% of the clinical antibody pipeline for immuno-oncology, immune-mediated and autoimmune disorders, diabetes, asthma, and rheumatoid arthritis. (pharmasalmanac.com)

There is clearly an urgent need for additional approaches and antibodies present new opportunities for both therapeutic and prophylactic intervention. (frontiersin.org)
Antibodies are widely recognized for their therapeutic potential and subsequently have prompted a lot of interest into their development and application. (ens-lyon.fr)
The therapeutic use of monoclonal antibodies consists in activation of antibody-dependent cell-mediated cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), or engagement with cell surface receptors (to either activate or inhibit signaling) (Figure 1 ). (ens-lyon.fr)

Now, the Barnes laboratory investigates viral-host interactions and translates knowledge of the structural correlates of antibody-mediated neutralization of viruses into the rational development of highly protective antibodies. (stanford.edu)
However, unlike protective antibodies to other viruses, antibodies to HSV do not prevent local recurrence(s). (medscape.com)

We find that mice without ABCs display defects in anti-viral IgG2a/c antibodies and are more susceptible to reactivation of γHV68 following virus challenges that typically do not break latency. (nature.com)

A one hundred fold boost inside the yield of the scFv antibody fragment was observed on co producing DnaK DnaJ GrpE, even though export of human granulocyte colony stimulating aspect, granulocyte macrophage colony stimulating element and interleukin 13 have been enormously improved upon production of DnaK and DnaJ. (screeninglibraries.com)

Each antiserum prepared showed maximal neutralizing activity against the homologous fitrate, but all antisera studied neutralized heterologous filtrates to some extent. (microbiologyresearch.org)

These parasites have been shown to alter the outcome to heterologous immunisation in a number of host species. (uky.edu)

Our findings demonstrate that a single intramuscular injection in mice of AAV encoding R1a-B6 fused to Fc fragments of different isotypes equipped either, with or without antibody dependent cellular cytotoxicity (ADCC) activity, was able to drive sustained high-level expression (0.5-1.1 mg/mL) in sera with no evidence of reduction for up to 6 months. (frontiersin.org)

Hapten-heterologous carrier recall of antihapten was successful as early as 37 days and as late as 11 months after sensitization. (caltech.edu)

In ChAd-primed participants, ChAd/Mod had the highest anti-spike IgG from day 28 through to 6 months, although the heterologous vs homologous geometric mean ratio (GMR) dropped from 9.7 (95%CI: 8.2,11.5) at D28 to 6.2 (95%CI: 5.0, 7.7) at D196. (jenner.ac.uk)

In this study, we investigated in vitro the potential of Trichoderma harzianum to produce bioactive secondary metabolites that can be used as alternatives to synthetic compounds. (bvsalud.org)
Étude de l'activité antibiotique 'in vitro' de la Pénicilline sur les bactéries acido-resistantes cultivées en milieux glycérines lipidolytiques. (microbiologyresearch.org)

A more practical and cost-effective strategy would be to use antibody gene therapy which would provide long term sustainable protection through antibody production within the patient. (frontiersin.org)
This poses the important question, which of these posttranslational modifications are naturally occurring in the yeast model or can be reconstituted by heterologous gene expression. (microbialcell.com)

Antibodies with different specificities to fipronil and its metabolites were produced. (cdc.gov)

Before boosting, the median % inhibition of neutralizing antibodies (NAbs) against the wild-type (WT), Alpha, Beta, Delta and Omicron variants in the ChAdOx-1 and BNT162b2 groups was 52.8% vs. 53.4, 36.6% vs. 39.9, 5.2% vs. 13.7, 34.3% vs. 44.9, and 20.8% vs. 18.8%, respectively. (biomedcentral.com)

Cross-subtype neutralizing single domain antibodies against influenza present new opportunities for immunoprophylaxis and pandemic preparedness. (frontiersin.org)
The emerging data on mixed schedules using the novel vaccine platforms deployed in the COVID-19 pandemic, suggest that heterologous priming schedules might be considered as a viable option sooner in future pandemics. (jenner.ac.uk)

Antibodies specific for both genotypes were detected, ranging from a prevalence of 6.6% to 44.8% for GIV.1 and from 6.8% to 15.1% for GIV.2 among different age groups. (cdc.gov)
Virus-specific IgM antibodies demonstrated in CSF. (cdc.gov)
Dengue-specific IgM antibodies present in serum with a P/N ratio ≥2. (cdc.gov)
Biochemical fractionation with phosphor-specific antibodies revealed the presence of phosphorylated Homer3 in the cytosolic fraction in contrast to high levels of nonphosphorylated Homer3 in PSD. (jneurosci.org)
Serum samples were then taken at various intervals and the specific activity of antibody was measured as soon as antibody reappeared in the circulation. (caltech.edu)
A comparison of antibody-specific activity provided evidence that there was a release of antibody which had been made at the time of S35 feeding and stored in some stabilized form in tissues. (caltech.edu)
Environmental factors that can detect specific IgE antibodies against most low-molecular- affect the initiation of occupational asthma include the intrinsic weight agents has resulted in a search for alternative or characteristics of causative agents as well as the influence of the complementary physiopathologic mechanisms leading to airway level and route of exposure at the workplace. (cdc.gov)
Disclosed herein are neutralizing antibodies with cross-neutralizing activity and cross-protective effects against divergent stains of influenza virus, which are specific for an epitope having at least 90% homology to amino acids +72-115 of the HA1 domain of H5N1 influenza virus hemagglutinin. (justia.com)
In one embodiment disclosed herein, a neutralizing antibody specific for an epitope having at least 90% homology to amino acids +72-115 of the HA1 domain of H5N1 influenza virus hemagglutinin is provided. (justia.com)
Also disclosed herein is a pharmaceutical formulation for neutralizing influenza virus comprising an antibody specific for an epitope having at least 90% homology to amino acids +72-115 of the HA1 domain of H5N1 influenza virus hemagglutinin. (justia.com)

BSIM group exhibited the lowest levels of anti-parasite antibodies. (fiocruz.br)
Individuals exposed to the parasite naturally generate antibodies that block a wide array of variants from attaching to CSA. (elifesciences.org)

4) Carry out biochemical and cell based analysis of algal expressed antibodies in comparison with their mammalian expressed counterparts. (grantome.com)

Moreover, their increasing application as host organisms for synthetic biology demonstrates the ease by which heterologous genes, such as the ones encoding enzmyes involved in posttranslational modifications, can be expressed and investigated in yeast. (microbialcell.com)

Analysis of antibodies against GIV.2 suggests zoonotic transmission of animal NoVs, likely attributable to interaction between humans and domestic pets. (cdc.gov)

Pups given birth to to PDK53-immunized dams also developed symptoms and displayed a median medical score of 4 (Number 1D), therefore suggesting failure of maternal antibodies to protect Rabbit polyclonal to PLEKHA9 against D2Y98P-PP1. (siamtech.net)

We were quite impressed that this vaccine candidate produced such a vigorous T-cell response in almost all trial participants who received the vaccine. (scienceboard.net)
antibody response of tropical range cattle to foot-and-mouth disease virus. (liverpool.ac.uk)
This antibody response requires approximately 7-10 days to develop and usually persists for greater than or equal to 2 years. (cdc.gov)

relationship between the chemical structure and the biological activity of antibodies against the foot-and-mouth disease virus]. (liverpool.ac.uk)

At present this potential is limited by the cost and time constraints in producing large quantities of these molecules. (grantome.com)
Applications of bispecific IgG-like antibodies and their target molecules. (ens-lyon.fr)

While investigating the pathogenesis of experimental nephritis produced by rabbit anti-rat-kidney antibody, it occurred to us that the effects of antikidney antibody on kidney tissue might readily be visualized in tissue cultures. (caltech.edu)
No evidence of naturally occurring anti-lipolytic antibody was found in any of the rabbit sera used. (microbiologyresearch.org)

Anatomy6

Organisms2

Chemicals and Drugs36

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Phenomena and Processes11

Disciplines and Occupations1

Information Science3

Health Care2

Titers11

Proteins14

MAbs15

Homologous and heterologous2

Broadly neutralizi3

Vaccination10

Levels of neutralisi1

Recombinant5

Vaccines9

Titer4

Challenge with heterologous2

ELISA5

Antigen14

Specificity5

Neutralizing antibody responses1

Strains6

Responses5

Monoclonal antibody2

Bispecific antibodies5

Antigens2

High-affinity1

Human antibody2

Serum antibodies2

Cross-reactive1

Booster2

Protein3

Purification4

Infection9

BNAbs1

Nanobody1

Immunogenicity1

Potent3

Immune response2

Induce2

Cells6

BsAbs1

Therapeutic3

Viruses2

IgG2a1

Fragment1

Antisera1

Immunisation1

Cellular1

Hapten1

Geometric mean1

Vitro2

Gene2

Specificities1

Inhibition1

Pandemic2

Specific10

Parasite2

Biochemical1

Genes1

Zoonotic1

Polyclonal1

Response3

Biological activity1

Molecules2

Rabbit2